GLG1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GLG1
Entrez Gene ID	2734
Full Name	golgi glycoprotein 1
Synonyms	CFR-1,ESL-1,MG-160,MG160
General protein information	Preferred Names golgi glycoprotein 1 Names Golgi apparatus protein 1 E-selectin ligand 1 cysteine-rich fibroblast growth factor receptor golgi sialoglycoprotein MG-160
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q23.1
Disorder MIM:	600753

mRNA and Protein(s)

mRNA	Protein	Name
NM_001145666.1	NP_001139138.1	Golgi apparatus protein 1 isoform 2 precursor
NM_001145667.2	NP_001139139.1	Golgi apparatus protein 1 isoform 3 precursor
NM_012201.5	NP_036333.2	Golgi apparatus protein 1 isoform 1 precursor
NM_001145666.2	NP_001139138.1	Golgi apparatus protein 1 isoform 2 precursor
NM_001145667.1	NP_001139139.1	Golgi apparatus protein 1 isoform 3 precursor
NM_012201.6	NP_036333.2	Golgi apparatus protein 1 isoform 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	GLG1	NP_036333.2
Canis lupus familiaris (dog)	GLG1	XP_536786.2
Bos taurus (cattle)	GLG1	NP_001231069.1
Rattus norvegicus (Norway rat)	Glg1	NP_058907.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001320	XP_321825.4
Pan troglodytes (chimpanzee)	GLG1	XP_511104.3
Mus musculus (house mouse)	Glg1	NP_033175.1
Danio rerio (zebrafish)	glg1a	XP_005163270.1
Gallus gallus (chicken)	GLG1	NP_990827.1
	GLG1	XP_002802614.1
Caenorhabditis elegans (roundworm)	CELE_F14E5.2	NP_001022088.1
Xenopus tropicalis (tropical clawed frog)	glg1	XP_004914112.1
Drosophila melanogaster (fruit fly)	Glg1	NP_001262163.1

Gene Ontology
Bibliography

Related articles in PubMed

Polysialylation and lipopolysaccharide-induced shedding of E-selectin ligand-1 and neuropilin-2 by microglia and THP-1 macrophages.
Werneburg S, Buettner FF, Erben L, Mathews M, Neumann H, M?hlenhoff M, Hildebrandt H
Glia64(8)1314-30(2016 Aug)

E-selectin ligand-1 (ESL-1) is a novel adiponectin binding protein on cell adhesion.
Yamamoto H, Kuroda N, Uekita H, Kochi I, Matsumoto A, Niinaga R, Funahashi T, Shimomura I, Kihara S
Biochemical and biophysical research communications470(2)425-430(2016 Feb)

E-selectin ligand-1 controls circulating prostate cancer cell rolling/adhesion and metastasis.
Yasmin-Karim S, King MR, Messing EM, Lee YF
Oncotarget5(23)12097-110(2014 Dec)

Meta-analysis identifies four new loci associated with testicular germ cell tumor.
Chung CC, Kanetsky PA, Wang Z, Hildebrandt MA, Koster R, Skotheim RI, Kratz CP, Turnbull C, Cortessis VK, Bakken AC, Bishop DT, Cook MB, Erickson RL, Foss? SD, Jacobs KB, Korde LA, Kraggerud SM, Lothe RA, Loud JT, Rahman N, Skinner EC, Thomas DC, Wu X, Yeager M, Schumacher FR, Greene MH, Schwartz SM, McGlynn KA, Chanock SJ, Nathanson KL
Nature genetics45(6)680-5(2013 Jun)

Leukocyte ligands for endothelial selectins: specialized glycoconjugates that mediate rolling and signaling under flow.
Zarbock A, Ley K, McEver RP, Hidalgo A
Blood118(26)6743-51(2011 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study identifies NRP2 and ESL-1 as targets for polysialylation in murine microglia and human THP-1 macrophages and reveals a striking convergence in the regulation of the two polysialylated acceptors in the course of inflammatory activation. A pool of polysialylated NRP2 and ESL-1 is assembled after injury or in culture and its shedding is an early response to lipopolysaccharide-induced activation of microglia.
Title: Polysialylation and lipopolysaccharide-induced shedding of E-selectin ligand-1 and neuropilin-2 by microglia and THP-1 macrophages.

Our results highlight the fact that interaction between APN and ESL-1 could provide a fundamental mechanism underlying the anti-atherogenic properties of APN.
Title: E-selectin ligand-1 (ESL-1) is a novel adiponectin binding protein on cell adhesion.

circulating prostate cancer cells' rolling capacity contributes to metastasis, and that is in part controlled by ESL-1.
Title: E-selectin ligand-1 controls circulating prostate cancer cell rolling/adhesion and metastasis.

Studies indicate that PSGL-1, CD44, and ESL-1 on mature leukocytes are physiologic glycoprotein ligands for endothelial P-selectin and E-selectin.
Title: Leukocyte ligands for endothelial selectins: specialized glycoconjugates that mediate rolling and signaling under flow.

expression of Cfr in the Golgi apparatus and on the plasma membrane is finely tuned through two distinct mechanisms for exhibiting different functions.
Title: Retention in the Golgi apparatus and expression on the cell surface of Cfr/Esl-1/Glg-1/MG-160 are regulated by two distinct mechanisms.

The following GLG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GLG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu10928	NM_012201.6 Latest version!	Homo sapiens golgi glycoprotein 1 (GLG1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu11607	NM_001145667.2 Latest version!	Homo sapiens golgi glycoprotein 1 (GLG1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$832.30 $1189.00
OHu11725	NM_001145666.2 Latest version!	Homo sapiens golgi glycoprotein 1 (GLG1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$839.30 $1199.00
View Old Accession Versions >>
OHu11725	NM_001145666.1	Homo sapiens golgi glycoprotein 1 (GLG1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$839.30 $1199.00
OHu11607	NM_001145667.1	Homo sapiens golgi glycoprotein 1 (GLG1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$832.30 $1189.00
OHu10928	NM_012201.5	Homo sapiens golgi glycoprotein 1 (GLG1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GLG1 ( NM_012201.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_036333.2 Homo sapiens golgi glycoprotein 1 (GLG1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu10928
Clone ID Related Accession (Same CDS sequence)	NM_012201.5 , NM_012201.6
Accession Version	NM_012201.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Homo sapiens(human)
Product	Golgi apparatus protein 1 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA493962.1, BC060822.1, AK304156.1, AC009053.7 and BX648396.1. On Mar 6, 2009 this sequence version replaced NM_012201.4. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC060822.1, SRR1660803.15929.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGT GTGGACGTGT ACGGAGGATG TTCCGCTTGT CGGCGGCGCT GCATCTGCTG 
CTGCTATTCG CGGCCGGGGC CGAGAAACTC CCCGGCCAGG GCGTCCACAG CCAGGGCCAG 
GGTCCCGGGG CCAACTTTGT GTCCTTCGTA GGGCAGGCCG GAGGCGGCGG CCCGGCGGGT 
CAGCAGCTGC CCCAGCTGCC TCAGTCATCG CAGCTTCAGC AGCAACAGCA GCAGCAGCAA 
CAGCAACAGC AGCCTCAGCC GCCGCAGCCG CCTTTCCCGG CGGGTGGGCC TCCGGCCCGG 
CGGGGAGGAG CGGGGGCTGG TGGGGGCTGG AAGCTGGCGG AGGAAGAGTC CTGCAGGGAG 
GACGTGACCC GCGTGTGCCC TAAGCACACC TGGAGCAACA ACCTGGCGGT GCTCGAGTGC 
CTGCAGGATG TGAGGGAGCC TGAAAATGAA ATTTCTTCAG ACTGCAATCA TTTGTTGTGG 
AATTATAAGC TGAACCTAAC TACAGATCCC AAATTTGAAT CTGTGGCCAG AGAGGTTTGC 
AAATCTACTA TAACAGAGAT TAAAGAATGT GCTGATGAAC CGGTTGGAAA AGGTTACATG 
GTTTCCTGCT TGGTGGATCA CCGAGGCAAC ATCACTGAGT ATCAGTGTCA CCAGTACATT 
ACCAAGATGA CGGCCATCAT TTTTAGTGAT TACCGTTTAA TCTGTGGCTT CATGGATGAC 
TGCAAAAATG ACATCAACAT TCTGAAATGT GGCAGTATTC GGCTTGGAGA AAAGGATGCA 
CATTCACAAG GTGAGGTGGT ATCATGCTTG GAGAAAGGCC TGGTGAAAGA AGCAGAAGAA 
AGAGAACCCA AGATTCAAGT TTCTGAACTC TGCAAGAAAG CCATTCTCCG GGTGGCTGAG 
CTGTCATCGG ATGACTTTCA CTTAGACCGG CATTTATATT TTGCTTGCCG AGATGATCGG 
GAGCGTTTTT GTGAAAATAC ACAAGCTGGT GAGGGCAGAG TGTATAAGTG CCTCTTTAAC 
CATAAATTTG AAGAATCCAT GAGTGAAAAG TGTCGAGAAG CACTTACAAC CCGCCAAAAG 
CTGATTGCCC AGGATTATAA AGTCAGTTAT TCATTGGCCA AATCCTGTAA AAGTGACTTG 
AAGAAATACC GGTGCAATGT GGAAAACCTT CCGCGATCGC GTGAAGCCAG GCTCTCCTAC 
TTGTTAATGT GCCTGGAGTC AGCTGTACAC AGAGGGCGAC AAGTCAGCAG TGAGTGCCAG 
GGGGAGATGC TGGATTACCG ACGCATGTTG ATGGAAGACT TTTCTCTGAG CCCTGAGATC 
ATCCTAAGCT GTCGGGGGGA GATTGAACAC CATTGTTCCG GATTACATCG AAAAGGGCGG 
ACCCTACACT GTCTGATGAA AGTAGTTCGA GGGGAGAAGG GGAACCTTGG AATGAACTGC 
CAGCAGGCGC TTCAAACACT GATTCAGGAG ACTGACCCTG GTGCAGATTA CCGCATTGAT 
CGAGCTTTGA ATGAAGCTTG TGAATCTGTA ATCCAGACAG CCTGCAAACA TATAAGATCT 
GGAGACCCAA TGATCTTGTC GTGCCTGATG GAACATTTAT ACACAGAGAA GATGGTAGAA 
GACTGTGAAC ACCGTCTCTT AGAGCTGCAG TATTTCATCT CCCGGGATTG GAAGCTGGAC 
CCTGTCCTGT ACCGCAAGTG CCAGGGAGAC GCTTCTCGTC TTTGCCACAC CCACGGTTGG 
AATGAGACCA GTGAATTTAT GCCTCAGGGA GCTGTGTTCT CTTGTTTATA CAGACACGCC 
TACCGCACTG AGGAACAGGG AAGGAGGCTC TCACGGGAGT GCCGAGCTGA AGTCCAAAGG 
ATCCTACACC AGCGTGCCAT GGATGTCAAG CTGGATCCTG CCCTCCAGGA TAAGTGCCTG 
ATTGATCTGG GAAAATGGTG CAGTGAGAAA ACAGAGACTG GACAGGAGCT GGAGTGCCTT 
CAGGACCATC TGGATGACTT GGTGGTGGAG TGTAGAGATA TAGTTGGCAA CCTCACTGAG 
TTAGAATCAG AGGATATTCA AATAGAAGCC TTGCTGATGA GAGCCTGTGA GCCCATAATT 
CAGAACTTCT GCCACGATGT GGCAGATAAC CAGATAGACT CTGGGGACCT GATGGAGTGT 
CTGATACAGA ACAAACACCA GAAGGACATG AACGAGAAGT GTGCCATCGG AGTTACCCAC 
TTCCAGCTGG TGCAGATGAA GGATTTTCGG TTTTCTTACA AGTTTAAAAT GGCCTGCAAG 
GAGGACGTGT TGAAGCTTTG CCCAAACATA AAAAAGAAGG TGGACGTGGT GATCTGCCTG 
AGCACGACCG TGCGCAATGA CACTCTGCAG GAAGCCAAGG AGCACAGGGT GTCCCTGAAG 
TGCCGCAGGC AGCTCCGTGT GGAGGAGCTG GAGATGACGG AGGACATCCG CTTGGAGCCA 
GATCTATACG AAGCCTGCAA GAGTGACATC AAAAACTTCT GTTCCGCTGT GCAATATGGC 
AACGCTCAGA TTATCGAATG TCTGAAAGAA AACAAGAAGC AGCTAAGCAC CCGCTGCCAC 
CAAAAAGTAT TTAAGCTGCA GGAGACAGAG ATGATGGACC CAGAGCTAGA CTACACCCTC 
ATGAGGGTCT GCAAGCAGAT GATAAAGAGG TTCTGTCCGG AAGCAGATTC TAAAACCATG 
TTGCAGTGCT TGAAGCAAAA TAAAAACAGT GAATTGATGG ATCCCAAATG CAAACAGATG 
ATAACCAAGC GCCAGATCAC CCAGAACACA GATTACCGCT TAAACCCCAT GTTAAGAAAA 
GCCTGTAAAG CTGACATTCC TAAATTCTGT CACGGTATCC TGACTAAGGC CAAGGATGAT 
TCAGAATTAG AAGGACAAGT CATCTCTTGC CTGAAGCTGA GATATGCTGA CCAGCGCCTG 
TCTTCAGACT GTGAAGACCA GATCCGAATC ATTATCCAGG AGTCCGCCCT GGACTACCGC 
CTGGATCCTC AGCTCCAGCT GCACTGCTCA GACGAGATCT CCAGTCTATG TGCTGAAGAA 
GCAGCAGCCC AAGAGCAGAC AGGTCAGGTG GAGGAGTGCC TCAAGGTCAA CCTGCTCAAG 
ATCAAAACAG AATTGTGTAA AAAGGAAGTG CTAAACATGC TGAAGGAAAG CAAAGCAGAC 
ATCTTTGTTG ACCCGGTACT TCATACTGCT TGTGCCCTGG ACATTAAACA CCACTGCGCA 
GCCATCACCC CTGGCCGCGG GCGTCAAATG TCCTGTCTCA TGGAAGCACT GGAGGATAAG 
CGGGTGAGGT TACAGCCCGA GTGCAAAAAG CGCCTCAATG ACCGGATTGA GATGTGGAGT 
TACGCAGCAA AGGTGGCCCC AGCAGATGGC TTCTCTGATC TTGCCATGCA AGTAATGACG 
TCTCCATCTA AGAACTACAT TCTCTCTGTG ATCAGTGGGA GCATCTGTAT ATTGTTCCTG 
ATTGGCCTGA TGTGTGGACG GATCACCAAG CGAGTGACAC GAGAGCTCAA GGACAGGCTA 
CAATACAGGT CAGAGACAAT GGCTTATAAA GGTTTAGTGT GGTCTCAGGA TGTGACAGGC 
AGTCCAGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036333.2
CDS	51..3662
Translation	MAACGRVRRMFRLSAALHLLLLFAAGAEKLPGQGVHSQGQGPGANFVSFVGQAGGGGPAGQQLPQLPQSSQLQQQQQQQQQQQQPQPPQPPFPAGGPPARRGGAGAGGGWKLAEEESCREDVTRVCPKHTWSNNLAVLECLQDVREPENEISSDCNHLLWNYKLNLTTDPKFESVAREVCKSTITEIKECADEPVGKGYMVSCLVDHRGNITEYQCHQYITKMTAIIFSDYRLICGFMDDCKNDINILKCGSIRLGEKDAHSQGEVVSCLEKGLVKEAEEREPKIQVSELCKKAILRVAELSSDDFHLDRHLYFACRDDRERFCENTQAGEGRVYKCLFNHKFEESMSEKCREALTTRQKLIAQDYKVSYSLAKSCKSDLKKYRCNVENLPRSREARLSYLLMCLESAVHRGRQVSSECQGEMLDYRRMLMEDFSLSPEIILSCRGEIEHHCSGLHRKGRTLHCLMKVVRGEKGNLGMNCQQALQTLIQETDPGADYRIDRALNEACESVIQTACKHIRSGDPMILSCLMEHLYTEKMVEDCEHRLLELQYFISRDWKLDPVLYRKCQGDASRLCHTHGWNETSEFMPQGAVFSCLYRHAYRTEEQGRRLSRECRAEVQRILHQRAMDVKLDPALQDKCLIDLGKWCSEKTETGQELECLQDHLDDLVVECRDIVGNLTELESEDIQIEALLMRACEPIIQNFCHDVADNQIDSGDLMECLIQNKHQKDMNEKCAIGVTHFQLVQMKDFRFSYKFKMACKEDVLKLCPNIKKKVDVVICLSTTVRNDTLQEAKEHRVSLKCRRQLRVEELEMTEDIRLEPDLYEACKSDIKNFCSAVQYGNAQIIECLKENKKQLSTRCHQKVFKLQETEMMDPELDYTLMRVCKQMIKRFCPEADSKTMLQCLKQNKNSELMDPKCKQMITKRQITQNTDYRLNPMLRKACKADIPKFCHGILTKAKDDSELEGQVISCLKLRYADQRLSSDCEDQIRIIIQESALDYRLDPQLQLHCSDEISSLCAEEAAAQEQTGQVEECLKVNLLKIKTELCKKEVLNMLKESKADIFVDPVLHTACALDIKHHCAAITPGRGRQMSCLMEALEDKRVRLQPECKKRLNDRIEMWSYAAKVAPADGFSDLAMQVMTSPSKNYILSVISGSICILFLIGLMCGRITKRVTRELKDRLQYRSETMAYKGLVWSQDVTGSPA

Target ORF information:

RefSeq Version	NM_012201.5
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi glycoprotein 1 (GLG1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012201.5

ORF Insert Sequence:

ATGGCGGCGT GTGGACGTGT ACGGAGGATG TTCCGCTTGT CGGCGGCGCT GCATCTGCTG 
CTGCTATTCG CGGCCGGGGC CGAGAAACTC CCCGGCCAGG GCGTCCACAG CCAGGGCCAG 
GGTCCCGGGG CCAACTTTGT GTCCTTCGTA GGGCAGGCCG GAGGCGGCGG CCCGGCGGGT 
CAGCAGCTGC CCCAGCTGCC TCAGTCATCG CAGCTTCAGC AGCAACAGCA GCAGCAGCAA 
CAGCAACAGC AGCCTCAGCC GCCGCAGCCG CCTTTCCCGG CGGGTGGGCC TCCGGCCCGG 
CGGGGAGGAG CGGGGGCTGG TGGGGGCTGG AAGCTGGCGG AGGAAGAGTC CTGCAGGGAG 
GACGTGACCC GCGTGTGCCC TAAGCACACC TGGAGCAACA ACCTGGCGGT GCTCGAGTGC 
CTGCAGGATG TGAGGGAGCC TGAAAATGAA ATTTCTTCAG ACTGCAATCA TTTGTTGTGG 
AATTATAAGC TGAACCTAAC TACAGATCCC AAATTTGAAT CTGTGGCCAG AGAGGTTTGC 
AAATCTACTA TAACAGAGAT TAAAGAATGT GCTGATGAAC CGGTTGGAAA AGGTTACATG 
GTTTCCTGCT TGGTGGATCA CCGAGGCAAC ATCACTGAGT ATCAGTGTCA CCAGTACATT 
ACCAAGATGA CGGCCATCAT TTTTAGTGAT TACCGTTTAA TCTGTGGCTT CATGGATGAC 
TGCAAAAATG ACATCAACAT TCTGAAATGT GGCAGTATTC GGCTTGGAGA AAAGGATGCA 
CATTCACAAG GTGAGGTGGT ATCATGCTTG GAGAAAGGCC TGGTGAAAGA AGCAGAAGAA 
AGAGAACCCA AGATTCAAGT TTCTGAACTC TGCAAGAAAG CCATTCTCCG GGTGGCTGAG 
CTGTCATCGG ATGACTTTCA CTTAGACCGG CATTTATATT TTGCTTGCCG AGATGATCGG 
GAGCGTTTTT GTGAAAATAC ACAAGCTGGT GAGGGCAGAG TGTATAAGTG CCTCTTTAAC 
CATAAATTTG AAGAATCCAT GAGTGAAAAG TGTCGAGAAG CACTTACAAC CCGCCAAAAG 
CTGATTGCCC AGGATTATAA AGTCAGTTAT TCATTGGCCA AATCCTGTAA AAGTGACTTG 
AAGAAATACC GGTGCAATGT GGAAAACCTT CCGCGATCGC GTGAAGCCAG GCTCTCCTAC 
TTGTTAATGT GCCTGGAGTC AGCTGTACAC AGAGGGCGAC AAGTCAGCAG TGAGTGCCAG 
GGGGAGATGC TGGATTACCG ACGCATGTTG ATGGAAGACT TTTCTCTGAG CCCTGAGATC 
ATCCTAAGCT GTCGGGGGGA GATTGAACAC CATTGTTCCG GATTACATCG AAAAGGGCGG 
ACCCTACACT GTCTGATGAA AGTAGTTCGA GGGGAGAAGG GGAACCTTGG AATGAACTGC 
CAGCAGGCGC TTCAAACACT GATTCAGGAG ACTGACCCTG GTGCAGATTA CCGCATTGAT 
CGAGCTTTGA ATGAAGCTTG TGAATCTGTA ATCCAGACAG CCTGCAAACA TATAAGATCT 
GGAGACCCAA TGATCTTGTC GTGCCTGATG GAACATTTAT ACACAGAGAA GATGGTAGAA 
GACTGTGAAC ACCGTCTCTT AGAGCTGCAG TATTTCATCT CCCGGGATTG GAAGCTGGAC 
CCTGTCCTGT ACCGCAAGTG CCAGGGAGAC GCTTCTCGTC TTTGCCACAC CCACGGTTGG 
AATGAGACCA GTGAATTTAT GCCTCAGGGA GCTGTGTTCT CTTGTTTATA CAGACACGCC 
TACCGCACTG AGGAACAGGG AAGGAGGCTC TCACGGGAGT GCCGAGCTGA AGTCCAAAGG 
ATCCTACACC AGCGTGCCAT GGATGTCAAG CTGGATCCTG CCCTCCAGGA TAAGTGCCTG 
ATTGATCTGG GAAAATGGTG CAGTGAGAAA ACAGAGACTG GACAGGAGCT GGAGTGCCTT 
CAGGACCATC TGGATGACTT GGTGGTGGAG TGTAGAGATA TAGTTGGCAA CCTCACTGAG 
TTAGAATCAG AGGATATTCA AATAGAAGCC TTGCTGATGA GAGCCTGTGA GCCCATAATT 
CAGAACTTCT GCCACGATGT GGCAGATAAC CAGATAGACT CTGGGGACCT GATGGAGTGT 
CTGATACAGA ACAAACACCA GAAGGACATG AACGAGAAGT GTGCCATCGG AGTTACCCAC 
TTCCAGCTGG TGCAGATGAA GGATTTTCGG TTTTCTTACA AGTTTAAAAT GGCCTGCAAG 
GAGGACGTGT TGAAGCTTTG CCCAAACATA AAAAAGAAGG TGGACGTGGT GATCTGCCTG 
AGCACGACCG TGCGCAATGA CACTCTGCAG GAAGCCAAGG AGCACAGGGT GTCCCTGAAG 
TGCCGCAGGC AGCTCCGTGT GGAGGAGCTG GAGATGACGG AGGACATCCG CTTGGAGCCA 
GATCTATACG AAGCCTGCAA GAGTGACATC AAAAACTTCT GTTCCGCTGT GCAATATGGC 
AACGCTCAGA TTATCGAATG TCTGAAAGAA AACAAGAAGC AGCTAAGCAC CCGCTGCCAC 
CAAAAAGTAT TTAAGCTGCA GGAGACAGAG ATGATGGACC CAGAGCTAGA CTACACCCTC 
ATGAGGGTCT GCAAGCAGAT GATAAAGAGG TTCTGTCCGG AAGCAGATTC TAAAACCATG 
TTGCAGTGCT TGAAGCAAAA TAAAAACAGT GAATTGATGG ATCCCAAATG CAAACAGATG 
ATAACCAAGC GCCAGATCAC CCAGAACACA GATTACCGCT TAAACCCCAT GTTAAGAAAA 
GCCTGTAAAG CTGACATTCC TAAATTCTGT CACGGTATCC TGACTAAGGC CAAGGATGAT 
TCAGAATTAG AAGGACAAGT CATCTCTTGC CTGAAGCTGA GATATGCTGA CCAGCGCCTG 
TCTTCAGACT GTGAAGACCA GATCCGAATC ATTATCCAGG AGTCCGCCCT GGACTACCGC 
CTGGATCCTC AGCTCCAGCT GCACTGCTCA GACGAGATCT CCAGTCTATG TGCTGAAGAA 
GCAGCAGCCC AAGAGCAGAC AGGTCAGGTG GAGGAGTGCC TCAAGGTCAA CCTGCTCAAG 
ATCAAAACAG AATTGTGTAA AAAGGAAGTG CTAAACATGC TGAAGGAAAG CAAAGCAGAC 
ATCTTTGTTG ACCCGGTACT TCATACTGCT TGTGCCCTGG ACATTAAACA CCACTGCGCA 
GCCATCACCC CTGGCCGCGG GCGTCAAATG TCCTGTCTCA TGGAAGCACT GGAGGATAAG 
CGGGTGAGGT TACAGCCCGA GTGCAAAAAG CGCCTCAATG ACCGGATTGA GATGTGGAGT 
TACGCAGCAA AGGTGGCCCC AGCAGATGGC TTCTCTGATC TTGCCATGCA AGTAATGACG 
TCTCCATCTA AGAACTACAT TCTCTCTGTG ATCAGTGGGA GCATCTGTAT ATTGTTCCTG 
ATTGGCCTGA TGTGTGGACG GATCACCAAG CGAGTGACAC GAGAGCTCAA GGACAGGCTA 
CAATACAGGT CAGAGACAAT GGCTTATAAA GGTTTAGTGT GGTCTCAGGA TGTGACAGGC 
AGTCCAGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLG1 ( NM_012201.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_036333.2 Homo sapiens golgi glycoprotein 1 (GLG1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu10928
Clone ID Related Accession (Same CDS sequence)	NM_012201.5 , NM_012201.6
Accession Version	NM_012201.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-28
Organism	Homo sapiens(human)
Product	Golgi apparatus protein 1 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC060822.1, AK304156.1, AC009053.7 and BX648396.1. On Jun 2, 2019 this sequence version replaced NM_012201.5. Transcript Variant: This variant (1) encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC060822.1, SRR1660803.15929.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGT GTGGACGTGT ACGGAGGATG TTCCGCTTGT CGGCGGCGCT GCATCTGCTG 
CTGCTATTCG CGGCCGGGGC CGAGAAACTC CCCGGCCAGG GCGTCCACAG CCAGGGCCAG 
GGTCCCGGGG CCAACTTTGT GTCCTTCGTA GGGCAGGCCG GAGGCGGCGG CCCGGCGGGT 
CAGCAGCTGC CCCAGCTGCC TCAGTCATCG CAGCTTCAGC AGCAACAGCA GCAGCAGCAA 
CAGCAACAGC AGCCTCAGCC GCCGCAGCCG CCTTTCCCGG CGGGTGGGCC TCCGGCCCGG 
CGGGGAGGAG CGGGGGCTGG TGGGGGCTGG AAGCTGGCGG AGGAAGAGTC CTGCAGGGAG 
GACGTGACCC GCGTGTGCCC TAAGCACACC TGGAGCAACA ACCTGGCGGT GCTCGAGTGC 
CTGCAGGATG TGAGGGAGCC TGAAAATGAA ATTTCTTCAG ACTGCAATCA TTTGTTGTGG 
AATTATAAGC TGAACCTAAC TACAGATCCC AAATTTGAAT CTGTGGCCAG AGAGGTTTGC 
AAATCTACTA TAACAGAGAT TAAAGAATGT GCTGATGAAC CGGTTGGAAA AGGTTACATG 
GTTTCCTGCT TGGTGGATCA CCGAGGCAAC ATCACTGAGT ATCAGTGTCA CCAGTACATT 
ACCAAGATGA CGGCCATCAT TTTTAGTGAT TACCGTTTAA TCTGTGGCTT CATGGATGAC 
TGCAAAAATG ACATCAACAT TCTGAAATGT GGCAGTATTC GGCTTGGAGA AAAGGATGCA 
CATTCACAAG GTGAGGTGGT ATCATGCTTG GAGAAAGGCC TGGTGAAAGA AGCAGAAGAA 
AGAGAACCCA AGATTCAAGT TTCTGAACTC TGCAAGAAAG CCATTCTCCG GGTGGCTGAG 
CTGTCATCGG ATGACTTTCA CTTAGACCGG CATTTATATT TTGCTTGCCG AGATGATCGG 
GAGCGTTTTT GTGAAAATAC ACAAGCTGGT GAGGGCAGAG TGTATAAGTG CCTCTTTAAC 
CATAAATTTG AAGAATCCAT GAGTGAAAAG TGTCGAGAAG CACTTACAAC CCGCCAAAAG 
CTGATTGCCC AGGATTATAA AGTCAGTTAT TCATTGGCCA AATCCTGTAA AAGTGACTTG 
AAGAAATACC GGTGCAATGT GGAAAACCTT CCGCGATCGC GTGAAGCCAG GCTCTCCTAC 
TTGTTAATGT GCCTGGAGTC AGCTGTACAC AGAGGGCGAC AAGTCAGCAG TGAGTGCCAG 
GGGGAGATGC TGGATTACCG ACGCATGTTG ATGGAAGACT TTTCTCTGAG CCCTGAGATC 
ATCCTAAGCT GTCGGGGGGA GATTGAACAC CATTGTTCCG GATTACATCG AAAAGGGCGG 
ACCCTACACT GTCTGATGAA AGTAGTTCGA GGGGAGAAGG GGAACCTTGG AATGAACTGC 
CAGCAGGCGC TTCAAACACT GATTCAGGAG ACTGACCCTG GTGCAGATTA CCGCATTGAT 
CGAGCTTTGA ATGAAGCTTG TGAATCTGTA ATCCAGACAG CCTGCAAACA TATAAGATCT 
GGAGACCCAA TGATCTTGTC GTGCCTGATG GAACATTTAT ACACAGAGAA GATGGTAGAA 
GACTGTGAAC ACCGTCTCTT AGAGCTGCAG TATTTCATCT CCCGGGATTG GAAGCTGGAC 
CCTGTCCTGT ACCGCAAGTG CCAGGGAGAC GCTTCTCGTC TTTGCCACAC CCACGGTTGG 
AATGAGACCA GTGAATTTAT GCCTCAGGGA GCTGTGTTCT CTTGTTTATA CAGACACGCC 
TACCGCACTG AGGAACAGGG AAGGAGGCTC TCACGGGAGT GCCGAGCTGA AGTCCAAAGG 
ATCCTACACC AGCGTGCCAT GGATGTCAAG CTGGATCCTG CCCTCCAGGA TAAGTGCCTG 
ATTGATCTGG GAAAATGGTG CAGTGAGAAA ACAGAGACTG GACAGGAGCT GGAGTGCCTT 
CAGGACCATC TGGATGACTT GGTGGTGGAG TGTAGAGATA TAGTTGGCAA CCTCACTGAG 
TTAGAATCAG AGGATATTCA AATAGAAGCC TTGCTGATGA GAGCCTGTGA GCCCATAATT 
CAGAACTTCT GCCACGATGT GGCAGATAAC CAGATAGACT CTGGGGACCT GATGGAGTGT 
CTGATACAGA ACAAACACCA GAAGGACATG AACGAGAAGT GTGCCATCGG AGTTACCCAC 
TTCCAGCTGG TGCAGATGAA GGATTTTCGG TTTTCTTACA AGTTTAAAAT GGCCTGCAAG 
GAGGACGTGT TGAAGCTTTG CCCAAACATA AAAAAGAAGG TGGACGTGGT GATCTGCCTG 
AGCACGACCG TGCGCAATGA CACTCTGCAG GAAGCCAAGG AGCACAGGGT GTCCCTGAAG 
TGCCGCAGGC AGCTCCGTGT GGAGGAGCTG GAGATGACGG AGGACATCCG CTTGGAGCCA 
GATCTATACG AAGCCTGCAA GAGTGACATC AAAAACTTCT GTTCCGCTGT GCAATATGGC 
AACGCTCAGA TTATCGAATG TCTGAAAGAA AACAAGAAGC AGCTAAGCAC CCGCTGCCAC 
CAAAAAGTAT TTAAGCTGCA GGAGACAGAG ATGATGGACC CAGAGCTAGA CTACACCCTC 
ATGAGGGTCT GCAAGCAGAT GATAAAGAGG TTCTGTCCGG AAGCAGATTC TAAAACCATG 
TTGCAGTGCT TGAAGCAAAA TAAAAACAGT GAATTGATGG ATCCCAAATG CAAACAGATG 
ATAACCAAGC GCCAGATCAC CCAGAACACA GATTACCGCT TAAACCCCAT GTTAAGAAAA 
GCCTGTAAAG CTGACATTCC TAAATTCTGT CACGGTATCC TGACTAAGGC CAAGGATGAT 
TCAGAATTAG AAGGACAAGT CATCTCTTGC CTGAAGCTGA GATATGCTGA CCAGCGCCTG 
TCTTCAGACT GTGAAGACCA GATCCGAATC ATTATCCAGG AGTCCGCCCT GGACTACCGC 
CTGGATCCTC AGCTCCAGCT GCACTGCTCA GACGAGATCT CCAGTCTATG TGCTGAAGAA 
GCAGCAGCCC AAGAGCAGAC AGGTCAGGTG GAGGAGTGCC TCAAGGTCAA CCTGCTCAAG 
ATCAAAACAG AATTGTGTAA AAAGGAAGTG CTAAACATGC TGAAGGAAAG CAAAGCAGAC 
ATCTTTGTTG ACCCGGTACT TCATACTGCT TGTGCCCTGG ACATTAAACA CCACTGCGCA 
GCCATCACCC CTGGCCGCGG GCGTCAAATG TCCTGTCTCA TGGAAGCACT GGAGGATAAG 
CGGGTGAGGT TACAGCCCGA GTGCAAAAAG CGCCTCAATG ACCGGATTGA GATGTGGAGT 
TACGCAGCAA AGGTGGCCCC AGCAGATGGC TTCTCTGATC TTGCCATGCA AGTAATGACG 
TCTCCATCTA AGAACTACAT TCTCTCTGTG ATCAGTGGGA GCATCTGTAT ATTGTTCCTG 
ATTGGCCTGA TGTGTGGACG GATCACCAAG CGAGTGACAC GAGAGCTCAA GGACAGGCTA 
CAATACAGGT CAGAGACAAT GGCTTATAAA GGTTTAGTGT GGTCTCAGGA TGTGACAGGC 
AGTCCAGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_036333.2
CDS	21..3632
Translation	MAACGRVRRMFRLSAALHLLLLFAAGAEKLPGQGVHSQGQGPGANFVSFVGQAGGGGPAGQQLPQLPQSSQLQQQQQQQQQQQQPQPPQPPFPAGGPPARRGGAGAGGGWKLAEEESCREDVTRVCPKHTWSNNLAVLECLQDVREPENEISSDCNHLLWNYKLNLTTDPKFESVAREVCKSTITEIKECADEPVGKGYMVSCLVDHRGNITEYQCHQYITKMTAIIFSDYRLICGFMDDCKNDINILKCGSIRLGEKDAHSQGEVVSCLEKGLVKEAEEREPKIQVSELCKKAILRVAELSSDDFHLDRHLYFACRDDRERFCENTQAGEGRVYKCLFNHKFEESMSEKCREALTTRQKLIAQDYKVSYSLAKSCKSDLKKYRCNVENLPRSREARLSYLLMCLESAVHRGRQVSSECQGEMLDYRRMLMEDFSLSPEIILSCRGEIEHHCSGLHRKGRTLHCLMKVVRGEKGNLGMNCQQALQTLIQETDPGADYRIDRALNEACESVIQTACKHIRSGDPMILSCLMEHLYTEKMVEDCEHRLLELQYFISRDWKLDPVLYRKCQGDASRLCHTHGWNETSEFMPQGAVFSCLYRHAYRTEEQGRRLSRECRAEVQRILHQRAMDVKLDPALQDKCLIDLGKWCSEKTETGQELECLQDHLDDLVVECRDIVGNLTELESEDIQIEALLMRACEPIIQNFCHDVADNQIDSGDLMECLIQNKHQKDMNEKCAIGVTHFQLVQMKDFRFSYKFKMACKEDVLKLCPNIKKKVDVVICLSTTVRNDTLQEAKEHRVSLKCRRQLRVEELEMTEDIRLEPDLYEACKSDIKNFCSAVQYGNAQIIECLKENKKQLSTRCHQKVFKLQETEMMDPELDYTLMRVCKQMIKRFCPEADSKTMLQCLKQNKNSELMDPKCKQMITKRQITQNTDYRLNPMLRKACKADIPKFCHGILTKAKDDSELEGQVISCLKLRYADQRLSSDCEDQIRIIIQESALDYRLDPQLQLHCSDEISSLCAEEAAAQEQTGQVEECLKVNLLKIKTELCKKEVLNMLKESKADIFVDPVLHTACALDIKHHCAAITPGRGRQMSCLMEALEDKRVRLQPECKKRLNDRIEMWSYAAKVAPADGFSDLAMQVMTSPSKNYILSVISGSICILFLIGLMCGRITKRVTRELKDRLQYRSETMAYKGLVWSQDVTGSPA

Target ORF information:

RefSeq Version	NM_012201.6
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi glycoprotein 1 (GLG1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012201.6

ORF Insert Sequence:

ATGGCGGCGT GTGGACGTGT ACGGAGGATG TTCCGCTTGT CGGCGGCGCT GCATCTGCTG 
CTGCTATTCG CGGCCGGGGC CGAGAAACTC CCCGGCCAGG GCGTCCACAG CCAGGGCCAG 
GGTCCCGGGG CCAACTTTGT GTCCTTCGTA GGGCAGGCCG GAGGCGGCGG CCCGGCGGGT 
CAGCAGCTGC CCCAGCTGCC TCAGTCATCG CAGCTTCAGC AGCAACAGCA GCAGCAGCAA 
CAGCAACAGC AGCCTCAGCC GCCGCAGCCG CCTTTCCCGG CGGGTGGGCC TCCGGCCCGG 
CGGGGAGGAG CGGGGGCTGG TGGGGGCTGG AAGCTGGCGG AGGAAGAGTC CTGCAGGGAG 
GACGTGACCC GCGTGTGCCC TAAGCACACC TGGAGCAACA ACCTGGCGGT GCTCGAGTGC 
CTGCAGGATG TGAGGGAGCC TGAAAATGAA ATTTCTTCAG ACTGCAATCA TTTGTTGTGG 
AATTATAAGC TGAACCTAAC TACAGATCCC AAATTTGAAT CTGTGGCCAG AGAGGTTTGC 
AAATCTACTA TAACAGAGAT TAAAGAATGT GCTGATGAAC CGGTTGGAAA AGGTTACATG 
GTTTCCTGCT TGGTGGATCA CCGAGGCAAC ATCACTGAGT ATCAGTGTCA CCAGTACATT 
ACCAAGATGA CGGCCATCAT TTTTAGTGAT TACCGTTTAA TCTGTGGCTT CATGGATGAC 
TGCAAAAATG ACATCAACAT TCTGAAATGT GGCAGTATTC GGCTTGGAGA AAAGGATGCA 
CATTCACAAG GTGAGGTGGT ATCATGCTTG GAGAAAGGCC TGGTGAAAGA AGCAGAAGAA 
AGAGAACCCA AGATTCAAGT TTCTGAACTC TGCAAGAAAG CCATTCTCCG GGTGGCTGAG 
CTGTCATCGG ATGACTTTCA CTTAGACCGG CATTTATATT TTGCTTGCCG AGATGATCGG 
GAGCGTTTTT GTGAAAATAC ACAAGCTGGT GAGGGCAGAG TGTATAAGTG CCTCTTTAAC 
CATAAATTTG AAGAATCCAT GAGTGAAAAG TGTCGAGAAG CACTTACAAC CCGCCAAAAG 
CTGATTGCCC AGGATTATAA AGTCAGTTAT TCATTGGCCA AATCCTGTAA AAGTGACTTG 
AAGAAATACC GGTGCAATGT GGAAAACCTT CCGCGATCGC GTGAAGCCAG GCTCTCCTAC 
TTGTTAATGT GCCTGGAGTC AGCTGTACAC AGAGGGCGAC AAGTCAGCAG TGAGTGCCAG 
GGGGAGATGC TGGATTACCG ACGCATGTTG ATGGAAGACT TTTCTCTGAG CCCTGAGATC 
ATCCTAAGCT GTCGGGGGGA GATTGAACAC CATTGTTCCG GATTACATCG AAAAGGGCGG 
ACCCTACACT GTCTGATGAA AGTAGTTCGA GGGGAGAAGG GGAACCTTGG AATGAACTGC 
CAGCAGGCGC TTCAAACACT GATTCAGGAG ACTGACCCTG GTGCAGATTA CCGCATTGAT 
CGAGCTTTGA ATGAAGCTTG TGAATCTGTA ATCCAGACAG CCTGCAAACA TATAAGATCT 
GGAGACCCAA TGATCTTGTC GTGCCTGATG GAACATTTAT ACACAGAGAA GATGGTAGAA 
GACTGTGAAC ACCGTCTCTT AGAGCTGCAG TATTTCATCT CCCGGGATTG GAAGCTGGAC 
CCTGTCCTGT ACCGCAAGTG CCAGGGAGAC GCTTCTCGTC TTTGCCACAC CCACGGTTGG 
AATGAGACCA GTGAATTTAT GCCTCAGGGA GCTGTGTTCT CTTGTTTATA CAGACACGCC 
TACCGCACTG AGGAACAGGG AAGGAGGCTC TCACGGGAGT GCCGAGCTGA AGTCCAAAGG 
ATCCTACACC AGCGTGCCAT GGATGTCAAG CTGGATCCTG CCCTCCAGGA TAAGTGCCTG 
ATTGATCTGG GAAAATGGTG CAGTGAGAAA ACAGAGACTG GACAGGAGCT GGAGTGCCTT 
CAGGACCATC TGGATGACTT GGTGGTGGAG TGTAGAGATA TAGTTGGCAA CCTCACTGAG 
TTAGAATCAG AGGATATTCA AATAGAAGCC TTGCTGATGA GAGCCTGTGA GCCCATAATT 
CAGAACTTCT GCCACGATGT GGCAGATAAC CAGATAGACT CTGGGGACCT GATGGAGTGT 
CTGATACAGA ACAAACACCA GAAGGACATG AACGAGAAGT GTGCCATCGG AGTTACCCAC 
TTCCAGCTGG TGCAGATGAA GGATTTTCGG TTTTCTTACA AGTTTAAAAT GGCCTGCAAG 
GAGGACGTGT TGAAGCTTTG CCCAAACATA AAAAAGAAGG TGGACGTGGT GATCTGCCTG 
AGCACGACCG TGCGCAATGA CACTCTGCAG GAAGCCAAGG AGCACAGGGT GTCCCTGAAG 
TGCCGCAGGC AGCTCCGTGT GGAGGAGCTG GAGATGACGG AGGACATCCG CTTGGAGCCA 
GATCTATACG AAGCCTGCAA GAGTGACATC AAAAACTTCT GTTCCGCTGT GCAATATGGC 
AACGCTCAGA TTATCGAATG TCTGAAAGAA AACAAGAAGC AGCTAAGCAC CCGCTGCCAC 
CAAAAAGTAT TTAAGCTGCA GGAGACAGAG ATGATGGACC CAGAGCTAGA CTACACCCTC 
ATGAGGGTCT GCAAGCAGAT GATAAAGAGG TTCTGTCCGG AAGCAGATTC TAAAACCATG 
TTGCAGTGCT TGAAGCAAAA TAAAAACAGT GAATTGATGG ATCCCAAATG CAAACAGATG 
ATAACCAAGC GCCAGATCAC CCAGAACACA GATTACCGCT TAAACCCCAT GTTAAGAAAA 
GCCTGTAAAG CTGACATTCC TAAATTCTGT CACGGTATCC TGACTAAGGC CAAGGATGAT 
TCAGAATTAG AAGGACAAGT CATCTCTTGC CTGAAGCTGA GATATGCTGA CCAGCGCCTG 
TCTTCAGACT GTGAAGACCA GATCCGAATC ATTATCCAGG AGTCCGCCCT GGACTACCGC 
CTGGATCCTC AGCTCCAGCT GCACTGCTCA GACGAGATCT CCAGTCTATG TGCTGAAGAA 
GCAGCAGCCC AAGAGCAGAC AGGTCAGGTG GAGGAGTGCC TCAAGGTCAA CCTGCTCAAG 
ATCAAAACAG AATTGTGTAA AAAGGAAGTG CTAAACATGC TGAAGGAAAG CAAAGCAGAC 
ATCTTTGTTG ACCCGGTACT TCATACTGCT TGTGCCCTGG ACATTAAACA CCACTGCGCA 
GCCATCACCC CTGGCCGCGG GCGTCAAATG TCCTGTCTCA TGGAAGCACT GGAGGATAAG 
CGGGTGAGGT TACAGCCCGA GTGCAAAAAG CGCCTCAATG ACCGGATTGA GATGTGGAGT 
TACGCAGCAA AGGTGGCCCC AGCAGATGGC TTCTCTGATC TTGCCATGCA AGTAATGACG 
TCTCCATCTA AGAACTACAT TCTCTCTGTG ATCAGTGGGA GCATCTGTAT ATTGTTCCTG 
ATTGGCCTGA TGTGTGGACG GATCACCAAG CGAGTGACAC GAGAGCTCAA GGACAGGCTA 
CAATACAGGT CAGAGACAAT GGCTTATAAA GGTTTAGTGT GGTCTCAGGA TGTGACAGGC 
AGTCCAGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLG1 ( NM_001145667.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001139139.1 Homo sapiens golgi glycoprotein 1 (GLG1), transcript variant 3, mRNA.

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CloneID	OHu11607
Clone ID Related Accession (Same CDS sequence)	NM_001145667.2 , NM_001145667.1
Accession Version	NM_001145667.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	Golgi apparatus protein 1 isoform 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC060822.1, U64791.1, AC009053.7 and BX648396.1. On Jun 2, 2019 this sequence version replaced NM_001145667.1. Transcript Variant: This variant (3) has an additional segment in the 3' region, as compared to variant 1. The resulting isoform (3) is shorter at the C-terminus, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.25761.1, SRR1803611.240918.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END##

ATGGCGGCGT GTGGACGTGT ACGGAGGATG TTCCGCTTGT CGGCGGCGCT GCATCTGCTG 
CTGCTATTCG CGGCCGGGGC CGAGAAACTC CCCGGCCAGG GCGTCCACAG CCAGGGCCAG 
GGTCCCGGGG CCAACTTTGT GTCCTTCGTA GGGCAGGCCG GAGGCGGCGG CCCGGCGGGT 
CAGCAGCTGC CCCAGCTGCC TCAGTCATCG CAGCTTCAGC AGCAACAGCA GCAGCAGCAA 
CAGCAACAGC AGCCTCAGCC GCCGCAGCCG CCTTTCCCGG CGGGTGGGCC TCCGGCCCGG 
CGGGGAGGAG CGGGGGCTGG TGGGGGCTGG AAGCTGGCGG AGGAAGAGTC CTGCAGGGAG 
GACGTGACCC GCGTGTGCCC TAAGCACACC TGGAGCAACA ACCTGGCGGT GCTCGAGTGC 
CTGCAGGATG TGAGGGAGCC TGAAAATGAA ATTTCTTCAG ACTGCAATCA TTTGTTGTGG 
AATTATAAGC TGAACCTAAC TACAGATCCC AAATTTGAAT CTGTGGCCAG AGAGGTTTGC 
AAATCTACTA TAACAGAGAT TAAAGAATGT GCTGATGAAC CGGTTGGAAA AGGTTACATG 
GTTTCCTGCT TGGTGGATCA CCGAGGCAAC ATCACTGAGT ATCAGTGTCA CCAGTACATT 
ACCAAGATGA CGGCCATCAT TTTTAGTGAT TACCGTTTAA TCTGTGGCTT CATGGATGAC 
TGCAAAAATG ACATCAACAT TCTGAAATGT GGCAGTATTC GGCTTGGAGA AAAGGATGCA 
CATTCACAAG GTGAGGTGGT ATCATGCTTG GAGAAAGGCC TGGTGAAAGA AGCAGAAGAA 
AGAGAACCCA AGATTCAAGT TTCTGAACTC TGCAAGAAAG CCATTCTCCG GGTGGCTGAG 
CTGTCATCGG ATGACTTTCA CTTAGACCGG CATTTATATT TTGCTTGCCG AGATGATCGG 
GAGCGTTTTT GTGAAAATAC ACAAGCTGGT GAGGGCAGAG TGTATAAGTG CCTCTTTAAC 
CATAAATTTG AAGAATCCAT GAGTGAAAAG TGTCGAGAAG CACTTACAAC CCGCCAAAAG 
CTGATTGCCC AGGATTATAA AGTCAGTTAT TCATTGGCCA AATCCTGTAA AAGTGACTTG 
AAGAAATACC GGTGCAATGT GGAAAACCTT CCGCGATCGC GTGAAGCCAG GCTCTCCTAC 
TTGTTAATGT GCCTGGAGTC AGCTGTACAC AGAGGGCGAC AAGTCAGCAG TGAGTGCCAG 
GGGGAGATGC TGGATTACCG ACGCATGTTG ATGGAAGACT TTTCTCTGAG CCCTGAGATC 
ATCCTAAGCT GTCGGGGGGA GATTGAACAC CATTGTTCCG GATTACATCG AAAAGGGCGG 
ACCCTACACT GTCTGATGAA AGTAGTTCGA GGGGAGAAGG GGAACCTTGG AATGAACTGC 
CAGCAGGCGC TTCAAACACT GATTCAGGAG ACTGACCCTG GTGCAGATTA CCGCATTGAT 
CGAGCTTTGA ATGAAGCTTG TGAATCTGTA ATCCAGACAG CCTGCAAACA TATAAGATCT 
GGAGACCCAA TGATCTTGTC GTGCCTGATG GAACATTTAT ACACAGAGAA GATGGTAGAA 
GACTGTGAAC ACCGTCTCTT AGAGCTGCAG TATTTCATCT CCCGGGATTG GAAGCTGGAC 
CCTGTCCTGT ACCGCAAGTG CCAGGGAGAC GCTTCTCGTC TTTGCCACAC CCACGGTTGG 
AATGAGACCA GTGAATTTAT GCCTCAGGGA GCTGTGTTCT CTTGTTTATA CAGACACGCC 
TACCGCACTG AGGAACAGGG AAGGAGGCTC TCACGGGAGT GCCGAGCTGA AGTCCAAAGG 
ATCCTACACC AGCGTGCCAT GGATGTCAAG CTGGATCCTG CCCTCCAGGA TAAGTGCCTG 
ATTGATCTGG GAAAATGGTG CAGTGAGAAA ACAGAGACTG GACAGGAGCT GGAGTGCCTT 
CAGGACCATC TGGATGACTT GGTGGTGGAG TGTAGAGATA TAGTTGGCAA CCTCACTGAG 
TTAGAATCAG AGGATATTCA AATAGAAGCC TTGCTGATGA GAGCCTGTGA GCCCATAATT 
CAGAACTTCT GCCACGATGT GGCAGATAAC CAGATAGACT CTGGGGACCT GATGGAGTGT 
CTGATACAGA ACAAACACCA GAAGGACATG AACGAGAAGT GTGCCATCGG AGTTACCCAC 
TTCCAGCTGG TGCAGATGAA GGATTTTCGG TTTTCTTACA AGTTTAAAAT GGCCTGCAAG 
GAGGACGTGT TGAAGCTTTG CCCAAACATA AAAAAGAAGG TGGACGTGGT GATCTGCCTG 
AGCACGACCG TGCGCAATGA CACTCTGCAG GAAGCCAAGG AGCACAGGGT GTCCCTGAAG 
TGCCGCAGGC AGCTCCGTGT GGAGGAGCTG GAGATGACGG AGGACATCCG CTTGGAGCCA 
GATCTATACG AAGCCTGCAA GAGTGACATC AAAAACTTCT GTTCCGCTGT GCAATATGGC 
AACGCTCAGA TTATCGAATG TCTGAAAGAA AACAAGAAGC AGCTAAGCAC CCGCTGCCAC 
CAAAAAGTAT TTAAGCTGCA GGAGACAGAG ATGATGGACC CAGAGCTAGA CTACACCCTC 
ATGAGGGTCT GCAAGCAGAT GATAAAGAGG TTCTGTCCGG AAGCAGATTC TAAAACCATG 
TTGCAGTGCT TGAAGCAAAA TAAAAACAGT GAATTGATGG ATCCCAAATG CAAACAGATG 
ATAACCAAGC GCCAGATCAC CCAGAACACA GATTACCGCT TAAACCCCAT GTTAAGAAAA 
GCCTGTAAAG CTGACATTCC TAAATTCTGT CACGGTATCC TGACTAAGGC CAAGGATGAT 
TCAGAATTAG AAGGACAAGT CATCTCTTGC CTGAAGCTGA GATATGCTGA CCAGCGCCTG 
TCTTCAGACT GTGAAGACCA GATCCGAATC ATTATCCAGG AGTCCGCCCT GGACTACCGC 
CTGGATCCTC AGCTCCAGCT GCACTGCTCA GACGAGATCT CCAGTCTATG TGCTGAAGAA 
GCAGCAGCCC AAGAGCAGAC AGGTCAGGTG GAGGAGTGCC TCAAGGTCAA CCTGCTCAAG 
ATCAAAACAG AATTGTGTAA AAAGGAAGTG CTAAACATGC TGAAGGAAAG CAAAGCAGAC 
ATCTTTGTTG ACCCGGTACT TCATACTGCT TGTGCCCTGG ACATTAAACA CCACTGCGCA 
GCCATCACCC CTGGCCGCGG GCGTCAAATG TCCTGTCTCA TGGAAGCACT GGAGGATAAG 
CGGGTGAGGT TACAGCCCGA GTGCAAAAAG CGCCTCAATG ACCGGATTGA GATGTGGAGT 
TACGCAGCAA AGGTGGCCCC AGCAGATGGC TTCTCTGATC TTGCCATGCA AGTAATGACG 
TCTCCATCTA AGAACTACAT TCTCTCTGTG ATCAGTGGGA GCATCTGTAT ATTGTTCCTG 
ATTGGCCTGA TGTGTGGACG GATCACCAAG CGAGTGACAC GAGAGCTCAA GGACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139139.1
CDS	21..3560
Translation	MAACGRVRRMFRLSAALHLLLLFAAGAEKLPGQGVHSQGQGPGANFVSFVGQAGGGGPAGQQLPQLPQSSQLQQQQQQQQQQQQPQPPQPPFPAGGPPARRGGAGAGGGWKLAEEESCREDVTRVCPKHTWSNNLAVLECLQDVREPENEISSDCNHLLWNYKLNLTTDPKFESVAREVCKSTITEIKECADEPVGKGYMVSCLVDHRGNITEYQCHQYITKMTAIIFSDYRLICGFMDDCKNDINILKCGSIRLGEKDAHSQGEVVSCLEKGLVKEAEEREPKIQVSELCKKAILRVAELSSDDFHLDRHLYFACRDDRERFCENTQAGEGRVYKCLFNHKFEESMSEKCREALTTRQKLIAQDYKVSYSLAKSCKSDLKKYRCNVENLPRSREARLSYLLMCLESAVHRGRQVSSECQGEMLDYRRMLMEDFSLSPEIILSCRGEIEHHCSGLHRKGRTLHCLMKVVRGEKGNLGMNCQQALQTLIQETDPGADYRIDRALNEACESVIQTACKHIRSGDPMILSCLMEHLYTEKMVEDCEHRLLELQYFISRDWKLDPVLYRKCQGDASRLCHTHGWNETSEFMPQGAVFSCLYRHAYRTEEQGRRLSRECRAEVQRILHQRAMDVKLDPALQDKCLIDLGKWCSEKTETGQELECLQDHLDDLVVECRDIVGNLTELESEDIQIEALLMRACEPIIQNFCHDVADNQIDSGDLMECLIQNKHQKDMNEKCAIGVTHFQLVQMKDFRFSYKFKMACKEDVLKLCPNIKKKVDVVICLSTTVRNDTLQEAKEHRVSLKCRRQLRVEELEMTEDIRLEPDLYEACKSDIKNFCSAVQYGNAQIIECLKENKKQLSTRCHQKVFKLQETEMMDPELDYTLMRVCKQMIKRFCPEADSKTMLQCLKQNKNSELMDPKCKQMITKRQITQNTDYRLNPMLRKACKADIPKFCHGILTKAKDDSELEGQVISCLKLRYADQRLSSDCEDQIRIIIQESALDYRLDPQLQLHCSDEISSLCAEEAAAQEQTGQVEECLKVNLLKIKTELCKKEVLNMLKESKADIFVDPVLHTACALDIKHHCAAITPGRGRQMSCLMEALEDKRVRLQPECKKRLNDRIEMWSYAAKVAPADGFSDLAMQVMTSPSKNYILSVISGSICILFLIGLMCGRITKRVTRELKDR

Target ORF information:

RefSeq Version	NM_001145667.2
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi glycoprotein 1 (GLG1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145667.2

ORF Insert Sequence:

ATGGCGGCGT GTGGACGTGT ACGGAGGATG TTCCGCTTGT CGGCGGCGCT GCATCTGCTG 
CTGCTATTCG CGGCCGGGGC CGAGAAACTC CCCGGCCAGG GCGTCCACAG CCAGGGCCAG 
GGTCCCGGGG CCAACTTTGT GTCCTTCGTA GGGCAGGCCG GAGGCGGCGG CCCGGCGGGT 
CAGCAGCTGC CCCAGCTGCC TCAGTCATCG CAGCTTCAGC AGCAACAGCA GCAGCAGCAA 
CAGCAACAGC AGCCTCAGCC GCCGCAGCCG CCTTTCCCGG CGGGTGGGCC TCCGGCCCGG 
CGGGGAGGAG CGGGGGCTGG TGGGGGCTGG AAGCTGGCGG AGGAAGAGTC CTGCAGGGAG 
GACGTGACCC GCGTGTGCCC TAAGCACACC TGGAGCAACA ACCTGGCGGT GCTCGAGTGC 
CTGCAGGATG TGAGGGAGCC TGAAAATGAA ATTTCTTCAG ACTGCAATCA TTTGTTGTGG 
AATTATAAGC TGAACCTAAC TACAGATCCC AAATTTGAAT CTGTGGCCAG AGAGGTTTGC 
AAATCTACTA TAACAGAGAT TAAAGAATGT GCTGATGAAC CGGTTGGAAA AGGTTACATG 
GTTTCCTGCT TGGTGGATCA CCGAGGCAAC ATCACTGAGT ATCAGTGTCA CCAGTACATT 
ACCAAGATGA CGGCCATCAT TTTTAGTGAT TACCGTTTAA TCTGTGGCTT CATGGATGAC 
TGCAAAAATG ACATCAACAT TCTGAAATGT GGCAGTATTC GGCTTGGAGA AAAGGATGCA 
CATTCACAAG GTGAGGTGGT ATCATGCTTG GAGAAAGGCC TGGTGAAAGA AGCAGAAGAA 
AGAGAACCCA AGATTCAAGT TTCTGAACTC TGCAAGAAAG CCATTCTCCG GGTGGCTGAG 
CTGTCATCGG ATGACTTTCA CTTAGACCGG CATTTATATT TTGCTTGCCG AGATGATCGG 
GAGCGTTTTT GTGAAAATAC ACAAGCTGGT GAGGGCAGAG TGTATAAGTG CCTCTTTAAC 
CATAAATTTG AAGAATCCAT GAGTGAAAAG TGTCGAGAAG CACTTACAAC CCGCCAAAAG 
CTGATTGCCC AGGATTATAA AGTCAGTTAT TCATTGGCCA AATCCTGTAA AAGTGACTTG 
AAGAAATACC GGTGCAATGT GGAAAACCTT CCGCGATCGC GTGAAGCCAG GCTCTCCTAC 
TTGTTAATGT GCCTGGAGTC AGCTGTACAC AGAGGGCGAC AAGTCAGCAG TGAGTGCCAG 
GGGGAGATGC TGGATTACCG ACGCATGTTG ATGGAAGACT TTTCTCTGAG CCCTGAGATC 
ATCCTAAGCT GTCGGGGGGA GATTGAACAC CATTGTTCCG GATTACATCG AAAAGGGCGG 
ACCCTACACT GTCTGATGAA AGTAGTTCGA GGGGAGAAGG GGAACCTTGG AATGAACTGC 
CAGCAGGCGC TTCAAACACT GATTCAGGAG ACTGACCCTG GTGCAGATTA CCGCATTGAT 
CGAGCTTTGA ATGAAGCTTG TGAATCTGTA ATCCAGACAG CCTGCAAACA TATAAGATCT 
GGAGACCCAA TGATCTTGTC GTGCCTGATG GAACATTTAT ACACAGAGAA GATGGTAGAA 
GACTGTGAAC ACCGTCTCTT AGAGCTGCAG TATTTCATCT CCCGGGATTG GAAGCTGGAC 
CCTGTCCTGT ACCGCAAGTG CCAGGGAGAC GCTTCTCGTC TTTGCCACAC CCACGGTTGG 
AATGAGACCA GTGAATTTAT GCCTCAGGGA GCTGTGTTCT CTTGTTTATA CAGACACGCC 
TACCGCACTG AGGAACAGGG AAGGAGGCTC TCACGGGAGT GCCGAGCTGA AGTCCAAAGG 
ATCCTACACC AGCGTGCCAT GGATGTCAAG CTGGATCCTG CCCTCCAGGA TAAGTGCCTG 
ATTGATCTGG GAAAATGGTG CAGTGAGAAA ACAGAGACTG GACAGGAGCT GGAGTGCCTT 
CAGGACCATC TGGATGACTT GGTGGTGGAG TGTAGAGATA TAGTTGGCAA CCTCACTGAG 
TTAGAATCAG AGGATATTCA AATAGAAGCC TTGCTGATGA GAGCCTGTGA GCCCATAATT 
CAGAACTTCT GCCACGATGT GGCAGATAAC CAGATAGACT CTGGGGACCT GATGGAGTGT 
CTGATACAGA ACAAACACCA GAAGGACATG AACGAGAAGT GTGCCATCGG AGTTACCCAC 
TTCCAGCTGG TGCAGATGAA GGATTTTCGG TTTTCTTACA AGTTTAAAAT GGCCTGCAAG 
GAGGACGTGT TGAAGCTTTG CCCAAACATA AAAAAGAAGG TGGACGTGGT GATCTGCCTG 
AGCACGACCG TGCGCAATGA CACTCTGCAG GAAGCCAAGG AGCACAGGGT GTCCCTGAAG 
TGCCGCAGGC AGCTCCGTGT GGAGGAGCTG GAGATGACGG AGGACATCCG CTTGGAGCCA 
GATCTATACG AAGCCTGCAA GAGTGACATC AAAAACTTCT GTTCCGCTGT GCAATATGGC 
AACGCTCAGA TTATCGAATG TCTGAAAGAA AACAAGAAGC AGCTAAGCAC CCGCTGCCAC 
CAAAAAGTAT TTAAGCTGCA GGAGACAGAG ATGATGGACC CAGAGCTAGA CTACACCCTC 
ATGAGGGTCT GCAAGCAGAT GATAAAGAGG TTCTGTCCGG AAGCAGATTC TAAAACCATG 
TTGCAGTGCT TGAAGCAAAA TAAAAACAGT GAATTGATGG ATCCCAAATG CAAACAGATG 
ATAACCAAGC GCCAGATCAC CCAGAACACA GATTACCGCT TAAACCCCAT GTTAAGAAAA 
GCCTGTAAAG CTGACATTCC TAAATTCTGT CACGGTATCC TGACTAAGGC CAAGGATGAT 
TCAGAATTAG AAGGACAAGT CATCTCTTGC CTGAAGCTGA GATATGCTGA CCAGCGCCTG 
TCTTCAGACT GTGAAGACCA GATCCGAATC ATTATCCAGG AGTCCGCCCT GGACTACCGC 
CTGGATCCTC AGCTCCAGCT GCACTGCTCA GACGAGATCT CCAGTCTATG TGCTGAAGAA 
GCAGCAGCCC AAGAGCAGAC AGGTCAGGTG GAGGAGTGCC TCAAGGTCAA CCTGCTCAAG 
ATCAAAACAG AATTGTGTAA AAAGGAAGTG CTAAACATGC TGAAGGAAAG CAAAGCAGAC 
ATCTTTGTTG ACCCGGTACT TCATACTGCT TGTGCCCTGG ACATTAAACA CCACTGCGCA 
GCCATCACCC CTGGCCGCGG GCGTCAAATG TCCTGTCTCA TGGAAGCACT GGAGGATAAG 
CGGGTGAGGT TACAGCCCGA GTGCAAAAAG CGCCTCAATG ACCGGATTGA GATGTGGAGT 
TACGCAGCAA AGGTGGCCCC AGCAGATGGC TTCTCTGATC TTGCCATGCA AGTAATGACG 
TCTCCATCTA AGAACTACAT TCTCTCTGTG ATCAGTGGGA GCATCTGTAT ATTGTTCCTG 
ATTGGCCTGA TGTGTGGACG GATCACCAAG CGAGTGACAC GAGAGCTCAA GGACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLG1 ( NM_001145667.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001139139.1 Homo sapiens golgi glycoprotein 1 (GLG1), transcript variant 3, mRNA.

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CloneID	OHu11607
Clone ID Related Accession (Same CDS sequence)	NM_001145667.2 , NM_001145667.1
Accession Version	NM_001145667.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Homo sapiens(human)
Product	Golgi apparatus protein 1 isoform 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA493962.1, BC060822.1, U64791.1, AC009053.7 and BX648396.1. Transcript Variant: This variant (3) has an additional segment in the 3' region, as compared to variant 1. The resulting isoform (3) is shorter at the C-terminus, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.185916.1, SRR1660807.33738.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCGT GTGGACGTGT ACGGAGGATG TTCCGCTTGT CGGCGGCGCT GCATCTGCTG 
CTGCTATTCG CGGCCGGGGC CGAGAAACTC CCCGGCCAGG GCGTCCACAG CCAGGGCCAG 
GGTCCCGGGG CCAACTTTGT GTCCTTCGTA GGGCAGGCCG GAGGCGGCGG CCCGGCGGGT 
CAGCAGCTGC CCCAGCTGCC TCAGTCATCG CAGCTTCAGC AGCAACAGCA GCAGCAGCAA 
CAGCAACAGC AGCCTCAGCC GCCGCAGCCG CCTTTCCCGG CGGGTGGGCC TCCGGCCCGG 
CGGGGAGGAG CGGGGGCTGG TGGGGGCTGG AAGCTGGCGG AGGAAGAGTC CTGCAGGGAG 
GACGTGACCC GCGTGTGCCC TAAGCACACC TGGAGCAACA ACCTGGCGGT GCTCGAGTGC 
CTGCAGGATG TGAGGGAGCC TGAAAATGAA ATTTCTTCAG ACTGCAATCA TTTGTTGTGG 
AATTATAAGC TGAACCTAAC TACAGATCCC AAATTTGAAT CTGTGGCCAG AGAGGTTTGC 
AAATCTACTA TAACAGAGAT TAAAGAATGT GCTGATGAAC CGGTTGGAAA AGGTTACATG 
GTTTCCTGCT TGGTGGATCA CCGAGGCAAC ATCACTGAGT ATCAGTGTCA CCAGTACATT 
ACCAAGATGA CGGCCATCAT TTTTAGTGAT TACCGTTTAA TCTGTGGCTT CATGGATGAC 
TGCAAAAATG ACATCAACAT TCTGAAATGT GGCAGTATTC GGCTTGGAGA AAAGGATGCA 
CATTCACAAG GTGAGGTGGT ATCATGCTTG GAGAAAGGCC TGGTGAAAGA AGCAGAAGAA 
AGAGAACCCA AGATTCAAGT TTCTGAACTC TGCAAGAAAG CCATTCTCCG GGTGGCTGAG 
CTGTCATCGG ATGACTTTCA CTTAGACCGG CATTTATATT TTGCTTGCCG AGATGATCGG 
GAGCGTTTTT GTGAAAATAC ACAAGCTGGT GAGGGCAGAG TGTATAAGTG CCTCTTTAAC 
CATAAATTTG AAGAATCCAT GAGTGAAAAG TGTCGAGAAG CACTTACAAC CCGCCAAAAG 
CTGATTGCCC AGGATTATAA AGTCAGTTAT TCATTGGCCA AATCCTGTAA AAGTGACTTG 
AAGAAATACC GGTGCAATGT GGAAAACCTT CCGCGATCGC GTGAAGCCAG GCTCTCCTAC 
TTGTTAATGT GCCTGGAGTC AGCTGTACAC AGAGGGCGAC AAGTCAGCAG TGAGTGCCAG 
GGGGAGATGC TGGATTACCG ACGCATGTTG ATGGAAGACT TTTCTCTGAG CCCTGAGATC 
ATCCTAAGCT GTCGGGGGGA GATTGAACAC CATTGTTCCG GATTACATCG AAAAGGGCGG 
ACCCTACACT GTCTGATGAA AGTAGTTCGA GGGGAGAAGG GGAACCTTGG AATGAACTGC 
CAGCAGGCGC TTCAAACACT GATTCAGGAG ACTGACCCTG GTGCAGATTA CCGCATTGAT 
CGAGCTTTGA ATGAAGCTTG TGAATCTGTA ATCCAGACAG CCTGCAAACA TATAAGATCT 
GGAGACCCAA TGATCTTGTC GTGCCTGATG GAACATTTAT ACACAGAGAA GATGGTAGAA 
GACTGTGAAC ACCGTCTCTT AGAGCTGCAG TATTTCATCT CCCGGGATTG GAAGCTGGAC 
CCTGTCCTGT ACCGCAAGTG CCAGGGAGAC GCTTCTCGTC TTTGCCACAC CCACGGTTGG 
AATGAGACCA GTGAATTTAT GCCTCAGGGA GCTGTGTTCT CTTGTTTATA CAGACACGCC 
TACCGCACTG AGGAACAGGG AAGGAGGCTC TCACGGGAGT GCCGAGCTGA AGTCCAAAGG 
ATCCTACACC AGCGTGCCAT GGATGTCAAG CTGGATCCTG CCCTCCAGGA TAAGTGCCTG 
ATTGATCTGG GAAAATGGTG CAGTGAGAAA ACAGAGACTG GACAGGAGCT GGAGTGCCTT 
CAGGACCATC TGGATGACTT GGTGGTGGAG TGTAGAGATA TAGTTGGCAA CCTCACTGAG 
TTAGAATCAG AGGATATTCA AATAGAAGCC TTGCTGATGA GAGCCTGTGA GCCCATAATT 
CAGAACTTCT GCCACGATGT GGCAGATAAC CAGATAGACT CTGGGGACCT GATGGAGTGT 
CTGATACAGA ACAAACACCA GAAGGACATG AACGAGAAGT GTGCCATCGG AGTTACCCAC 
TTCCAGCTGG TGCAGATGAA GGATTTTCGG TTTTCTTACA AGTTTAAAAT GGCCTGCAAG 
GAGGACGTGT TGAAGCTTTG CCCAAACATA AAAAAGAAGG TGGACGTGGT GATCTGCCTG 
AGCACGACCG TGCGCAATGA CACTCTGCAG GAAGCCAAGG AGCACAGGGT GTCCCTGAAG 
TGCCGCAGGC AGCTCCGTGT GGAGGAGCTG GAGATGACGG AGGACATCCG CTTGGAGCCA 
GATCTATACG AAGCCTGCAA GAGTGACATC AAAAACTTCT GTTCCGCTGT GCAATATGGC 
AACGCTCAGA TTATCGAATG TCTGAAAGAA AACAAGAAGC AGCTAAGCAC CCGCTGCCAC 
CAAAAAGTAT TTAAGCTGCA GGAGACAGAG ATGATGGACC CAGAGCTAGA CTACACCCTC 
ATGAGGGTCT GCAAGCAGAT GATAAAGAGG TTCTGTCCGG AAGCAGATTC TAAAACCATG 
TTGCAGTGCT TGAAGCAAAA TAAAAACAGT GAATTGATGG ATCCCAAATG CAAACAGATG 
ATAACCAAGC GCCAGATCAC CCAGAACACA GATTACCGCT TAAACCCCAT GTTAAGAAAA 
GCCTGTAAAG CTGACATTCC TAAATTCTGT CACGGTATCC TGACTAAGGC CAAGGATGAT 
TCAGAATTAG AAGGACAAGT CATCTCTTGC CTGAAGCTGA GATATGCTGA CCAGCGCCTG 
TCTTCAGACT GTGAAGACCA GATCCGAATC ATTATCCAGG AGTCCGCCCT GGACTACCGC 
CTGGATCCTC AGCTCCAGCT GCACTGCTCA GACGAGATCT CCAGTCTATG TGCTGAAGAA 
GCAGCAGCCC AAGAGCAGAC AGGTCAGGTG GAGGAGTGCC TCAAGGTCAA CCTGCTCAAG 
ATCAAAACAG AATTGTGTAA AAAGGAAGTG CTAAACATGC TGAAGGAAAG CAAAGCAGAC 
ATCTTTGTTG ACCCGGTACT TCATACTGCT TGTGCCCTGG ACATTAAACA CCACTGCGCA 
GCCATCACCC CTGGCCGCGG GCGTCAAATG TCCTGTCTCA TGGAAGCACT GGAGGATAAG 
CGGGTGAGGT TACAGCCCGA GTGCAAAAAG CGCCTCAATG ACCGGATTGA GATGTGGAGT 
TACGCAGCAA AGGTGGCCCC AGCAGATGGC TTCTCTGATC TTGCCATGCA AGTAATGACG 
TCTCCATCTA AGAACTACAT TCTCTCTGTG ATCAGTGGGA GCATCTGTAT ATTGTTCCTG 
ATTGGCCTGA TGTGTGGACG GATCACCAAG CGAGTGACAC GAGAGCTCAA GGACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139139.1
CDS	51..3590
Translation	MAACGRVRRMFRLSAALHLLLLFAAGAEKLPGQGVHSQGQGPGANFVSFVGQAGGGGPAGQQLPQLPQSSQLQQQQQQQQQQQQPQPPQPPFPAGGPPARRGGAGAGGGWKLAEEESCREDVTRVCPKHTWSNNLAVLECLQDVREPENEISSDCNHLLWNYKLNLTTDPKFESVAREVCKSTITEIKECADEPVGKGYMVSCLVDHRGNITEYQCHQYITKMTAIIFSDYRLICGFMDDCKNDINILKCGSIRLGEKDAHSQGEVVSCLEKGLVKEAEEREPKIQVSELCKKAILRVAELSSDDFHLDRHLYFACRDDRERFCENTQAGEGRVYKCLFNHKFEESMSEKCREALTTRQKLIAQDYKVSYSLAKSCKSDLKKYRCNVENLPRSREARLSYLLMCLESAVHRGRQVSSECQGEMLDYRRMLMEDFSLSPEIILSCRGEIEHHCSGLHRKGRTLHCLMKVVRGEKGNLGMNCQQALQTLIQETDPGADYRIDRALNEACESVIQTACKHIRSGDPMILSCLMEHLYTEKMVEDCEHRLLELQYFISRDWKLDPVLYRKCQGDASRLCHTHGWNETSEFMPQGAVFSCLYRHAYRTEEQGRRLSRECRAEVQRILHQRAMDVKLDPALQDKCLIDLGKWCSEKTETGQELECLQDHLDDLVVECRDIVGNLTELESEDIQIEALLMRACEPIIQNFCHDVADNQIDSGDLMECLIQNKHQKDMNEKCAIGVTHFQLVQMKDFRFSYKFKMACKEDVLKLCPNIKKKVDVVICLSTTVRNDTLQEAKEHRVSLKCRRQLRVEELEMTEDIRLEPDLYEACKSDIKNFCSAVQYGNAQIIECLKENKKQLSTRCHQKVFKLQETEMMDPELDYTLMRVCKQMIKRFCPEADSKTMLQCLKQNKNSELMDPKCKQMITKRQITQNTDYRLNPMLRKACKADIPKFCHGILTKAKDDSELEGQVISCLKLRYADQRLSSDCEDQIRIIIQESALDYRLDPQLQLHCSDEISSLCAEEAAAQEQTGQVEECLKVNLLKIKTELCKKEVLNMLKESKADIFVDPVLHTACALDIKHHCAAITPGRGRQMSCLMEALEDKRVRLQPECKKRLNDRIEMWSYAAKVAPADGFSDLAMQVMTSPSKNYILSVISGSICILFLIGLMCGRITKRVTRELKDR

Target ORF information:

RefSeq Version	NM_001145667.1
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi glycoprotein 1 (GLG1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145667.1

ORF Insert Sequence:

ATGGCGGCGT GTGGACGTGT ACGGAGGATG TTCCGCTTGT CGGCGGCGCT GCATCTGCTG 
CTGCTATTCG CGGCCGGGGC CGAGAAACTC CCCGGCCAGG GCGTCCACAG CCAGGGCCAG 
GGTCCCGGGG CCAACTTTGT GTCCTTCGTA GGGCAGGCCG GAGGCGGCGG CCCGGCGGGT 
CAGCAGCTGC CCCAGCTGCC TCAGTCATCG CAGCTTCAGC AGCAACAGCA GCAGCAGCAA 
CAGCAACAGC AGCCTCAGCC GCCGCAGCCG CCTTTCCCGG CGGGTGGGCC TCCGGCCCGG 
CGGGGAGGAG CGGGGGCTGG TGGGGGCTGG AAGCTGGCGG AGGAAGAGTC CTGCAGGGAG 
GACGTGACCC GCGTGTGCCC TAAGCACACC TGGAGCAACA ACCTGGCGGT GCTCGAGTGC 
CTGCAGGATG TGAGGGAGCC TGAAAATGAA ATTTCTTCAG ACTGCAATCA TTTGTTGTGG 
AATTATAAGC TGAACCTAAC TACAGATCCC AAATTTGAAT CTGTGGCCAG AGAGGTTTGC 
AAATCTACTA TAACAGAGAT TAAAGAATGT GCTGATGAAC CGGTTGGAAA AGGTTACATG 
GTTTCCTGCT TGGTGGATCA CCGAGGCAAC ATCACTGAGT ATCAGTGTCA CCAGTACATT 
ACCAAGATGA CGGCCATCAT TTTTAGTGAT TACCGTTTAA TCTGTGGCTT CATGGATGAC 
TGCAAAAATG ACATCAACAT TCTGAAATGT GGCAGTATTC GGCTTGGAGA AAAGGATGCA 
CATTCACAAG GTGAGGTGGT ATCATGCTTG GAGAAAGGCC TGGTGAAAGA AGCAGAAGAA 
AGAGAACCCA AGATTCAAGT TTCTGAACTC TGCAAGAAAG CCATTCTCCG GGTGGCTGAG 
CTGTCATCGG ATGACTTTCA CTTAGACCGG CATTTATATT TTGCTTGCCG AGATGATCGG 
GAGCGTTTTT GTGAAAATAC ACAAGCTGGT GAGGGCAGAG TGTATAAGTG CCTCTTTAAC 
CATAAATTTG AAGAATCCAT GAGTGAAAAG TGTCGAGAAG CACTTACAAC CCGCCAAAAG 
CTGATTGCCC AGGATTATAA AGTCAGTTAT TCATTGGCCA AATCCTGTAA AAGTGACTTG 
AAGAAATACC GGTGCAATGT GGAAAACCTT CCGCGATCGC GTGAAGCCAG GCTCTCCTAC 
TTGTTAATGT GCCTGGAGTC AGCTGTACAC AGAGGGCGAC AAGTCAGCAG TGAGTGCCAG 
GGGGAGATGC TGGATTACCG ACGCATGTTG ATGGAAGACT TTTCTCTGAG CCCTGAGATC 
ATCCTAAGCT GTCGGGGGGA GATTGAACAC CATTGTTCCG GATTACATCG AAAAGGGCGG 
ACCCTACACT GTCTGATGAA AGTAGTTCGA GGGGAGAAGG GGAACCTTGG AATGAACTGC 
CAGCAGGCGC TTCAAACACT GATTCAGGAG ACTGACCCTG GTGCAGATTA CCGCATTGAT 
CGAGCTTTGA ATGAAGCTTG TGAATCTGTA ATCCAGACAG CCTGCAAACA TATAAGATCT 
GGAGACCCAA TGATCTTGTC GTGCCTGATG GAACATTTAT ACACAGAGAA GATGGTAGAA 
GACTGTGAAC ACCGTCTCTT AGAGCTGCAG TATTTCATCT CCCGGGATTG GAAGCTGGAC 
CCTGTCCTGT ACCGCAAGTG CCAGGGAGAC GCTTCTCGTC TTTGCCACAC CCACGGTTGG 
AATGAGACCA GTGAATTTAT GCCTCAGGGA GCTGTGTTCT CTTGTTTATA CAGACACGCC 
TACCGCACTG AGGAACAGGG AAGGAGGCTC TCACGGGAGT GCCGAGCTGA AGTCCAAAGG 
ATCCTACACC AGCGTGCCAT GGATGTCAAG CTGGATCCTG CCCTCCAGGA TAAGTGCCTG 
ATTGATCTGG GAAAATGGTG CAGTGAGAAA ACAGAGACTG GACAGGAGCT GGAGTGCCTT 
CAGGACCATC TGGATGACTT GGTGGTGGAG TGTAGAGATA TAGTTGGCAA CCTCACTGAG 
TTAGAATCAG AGGATATTCA AATAGAAGCC TTGCTGATGA GAGCCTGTGA GCCCATAATT 
CAGAACTTCT GCCACGATGT GGCAGATAAC CAGATAGACT CTGGGGACCT GATGGAGTGT 
CTGATACAGA ACAAACACCA GAAGGACATG AACGAGAAGT GTGCCATCGG AGTTACCCAC 
TTCCAGCTGG TGCAGATGAA GGATTTTCGG TTTTCTTACA AGTTTAAAAT GGCCTGCAAG 
GAGGACGTGT TGAAGCTTTG CCCAAACATA AAAAAGAAGG TGGACGTGGT GATCTGCCTG 
AGCACGACCG TGCGCAATGA CACTCTGCAG GAAGCCAAGG AGCACAGGGT GTCCCTGAAG 
TGCCGCAGGC AGCTCCGTGT GGAGGAGCTG GAGATGACGG AGGACATCCG CTTGGAGCCA 
GATCTATACG AAGCCTGCAA GAGTGACATC AAAAACTTCT GTTCCGCTGT GCAATATGGC 
AACGCTCAGA TTATCGAATG TCTGAAAGAA AACAAGAAGC AGCTAAGCAC CCGCTGCCAC 
CAAAAAGTAT TTAAGCTGCA GGAGACAGAG ATGATGGACC CAGAGCTAGA CTACACCCTC 
ATGAGGGTCT GCAAGCAGAT GATAAAGAGG TTCTGTCCGG AAGCAGATTC TAAAACCATG 
TTGCAGTGCT TGAAGCAAAA TAAAAACAGT GAATTGATGG ATCCCAAATG CAAACAGATG 
ATAACCAAGC GCCAGATCAC CCAGAACACA GATTACCGCT TAAACCCCAT GTTAAGAAAA 
GCCTGTAAAG CTGACATTCC TAAATTCTGT CACGGTATCC TGACTAAGGC CAAGGATGAT 
TCAGAATTAG AAGGACAAGT CATCTCTTGC CTGAAGCTGA GATATGCTGA CCAGCGCCTG 
TCTTCAGACT GTGAAGACCA GATCCGAATC ATTATCCAGG AGTCCGCCCT GGACTACCGC 
CTGGATCCTC AGCTCCAGCT GCACTGCTCA GACGAGATCT CCAGTCTATG TGCTGAAGAA 
GCAGCAGCCC AAGAGCAGAC AGGTCAGGTG GAGGAGTGCC TCAAGGTCAA CCTGCTCAAG 
ATCAAAACAG AATTGTGTAA AAAGGAAGTG CTAAACATGC TGAAGGAAAG CAAAGCAGAC 
ATCTTTGTTG ACCCGGTACT TCATACTGCT TGTGCCCTGG ACATTAAACA CCACTGCGCA 
GCCATCACCC CTGGCCGCGG GCGTCAAATG TCCTGTCTCA TGGAAGCACT GGAGGATAAG 
CGGGTGAGGT TACAGCCCGA GTGCAAAAAG CGCCTCAATG ACCGGATTGA GATGTGGAGT 
TACGCAGCAA AGGTGGCCCC AGCAGATGGC TTCTCTGATC TTGCCATGCA AGTAATGACG 
TCTCCATCTA AGAACTACAT TCTCTCTGTG ATCAGTGGGA GCATCTGTAT ATTGTTCCTG 
ATTGGCCTGA TGTGTGGACG GATCACCAAG CGAGTGACAC GAGAGCTCAA GGACAGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLG1 ( NM_001145666.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001139138.1 Homo sapiens golgi glycoprotein 1 (GLG1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu11725
Clone ID Related Accession (Same CDS sequence)	NM_001145666.1 , NM_001145666.2
Accession Version	NM_001145666.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Homo sapiens(human)
Product	Golgi apparatus protein 1 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA493962.1, AK304156.1, BC060822.1, AC009053.7 and BX648396.1. Transcript Variant: This variant (2) lacks an in-frame coding exon, as compared to variant 1. The resulting isoform (2) lacks an internal segment, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304156.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCGT GTGGACGTGT ACGGAGGATG TTCCGCTTGT CGGCGGCGCT GCATCTGCTG 
CTGCTATTCG CGGCCGGGGC CGAGAAACTC CCCGGCCAGG GCGTCCACAG CCAGGGCCAG 
GGTCCCGGGG CCAACTTTGT GTCCTTCGTA GGGCAGGCCG GAGGCGGCGG CCCGGCGGGT 
CAGCAGCTGC CCCAGCTGCC TCAGTCATCG CAGCTTCAGC AGCAACAGCA GCAGCAGCAA 
CAGCAACAGC AGCCTCAGCC GCCGCAGCCG CCTTTCCCGG CGGGTGGGCC TCCGGCCCGG 
CGGGGAGGAG CGGGGGCTGG TGGGGGCTGG AAGCTGGCGG AGGAAGAGTC CTGCAGGGAG 
GACGTGACCC GCGTGTGCCC TAAGCACACC TGGAGCAACA ACCTGGCGGT GCTCGAGTGC 
CTGCAGGATG TGAGGGAGTT GTTGTGGAAT TATAAGCTGA ACCTAACTAC AGATCCCAAA 
TTTGAATCTG TGGCCAGAGA GGTTTGCAAA TCTACTATAA CAGAGATTAA AGAATGTGCT 
GATGAACCGG TTGGAAAAGG TTACATGGTT TCCTGCTTGG TGGATCACCG AGGCAACATC 
ACTGAGTATC AGTGTCACCA GTACATTACC AAGATGACGG CCATCATTTT TAGTGATTAC 
CGTTTAATCT GTGGCTTCAT GGATGACTGC AAAAATGACA TCAACATTCT GAAATGTGGC 
AGTATTCGGC TTGGAGAAAA GGATGCACAT TCACAAGGTG AGGTGGTATC ATGCTTGGAG 
AAAGGCCTGG TGAAAGAAGC AGAAGAAAGA GAACCCAAGA TTCAAGTTTC TGAACTCTGC 
AAGAAAGCCA TTCTCCGGGT GGCTGAGCTG TCATCGGATG ACTTTCACTT AGACCGGCAT 
TTATATTTTG CTTGCCGAGA TGATCGGGAG CGTTTTTGTG AAAATACACA AGCTGGTGAG 
GGCAGAGTGT ATAAGTGCCT CTTTAACCAT AAATTTGAAG AATCCATGAG TGAAAAGTGT 
CGAGAAGCAC TTACAACCCG CCAAAAGCTG ATTGCCCAGG ATTATAAAGT CAGTTATTCA 
TTGGCCAAAT CCTGTAAAAG TGACTTGAAG AAATACCGGT GCAATGTGGA AAACCTTCCG 
CGATCGCGTG AAGCCAGGCT CTCCTACTTG TTAATGTGCC TGGAGTCAGC TGTACACAGA 
GGGCGACAAG TCAGCAGTGA GTGCCAGGGG GAGATGCTGG ATTACCGACG CATGTTGATG 
GAAGACTTTT CTCTGAGCCC TGAGATCATC CTAAGCTGTC GGGGGGAGAT TGAACACCAT 
TGTTCCGGAT TACATCGAAA AGGGCGGACC CTACACTGTC TGATGAAAGT AGTTCGAGGG 
GAGAAGGGGA ACCTTGGAAT GAACTGCCAG CAGGCGCTTC AAACACTGAT TCAGGAGACT 
GACCCTGGTG CAGATTACCG CATTGATCGA GCTTTGAATG AAGCTTGTGA ATCTGTAATC 
CAGACAGCCT GCAAACATAT AAGATCTGGA GACCCAATGA TCTTGTCGTG CCTGATGGAA 
CATTTATACA CAGAGAAGAT GGTAGAAGAC TGTGAACACC GTCTCTTAGA GCTGCAGTAT 
TTCATCTCCC GGGATTGGAA GCTGGACCCT GTCCTGTACC GCAAGTGCCA GGGAGACGCT 
TCTCGTCTTT GCCACACCCA CGGTTGGAAT GAGACCAGTG AATTTATGCC TCAGGGAGCT 
GTGTTCTCTT GTTTATACAG ACACGCCTAC CGCACTGAGG AACAGGGAAG GAGGCTCTCA 
CGGGAGTGCC GAGCTGAAGT CCAAAGGATC CTACACCAGC GTGCCATGGA TGTCAAGCTG 
GATCCTGCCC TCCAGGATAA GTGCCTGATT GATCTGGGAA AATGGTGCAG TGAGAAAACA 
GAGACTGGAC AGGAGCTGGA GTGCCTTCAG GACCATCTGG ATGACTTGGT GGTGGAGTGT 
AGAGATATAG TTGGCAACCT CACTGAGTTA GAATCAGAGG ATATTCAAAT AGAAGCCTTG 
CTGATGAGAG CCTGTGAGCC CATAATTCAG AACTTCTGCC ACGATGTGGC AGATAACCAG 
ATAGACTCTG GGGACCTGAT GGAGTGTCTG ATACAGAACA AACACCAGAA GGACATGAAC 
GAGAAGTGTG CCATCGGAGT TACCCACTTC CAGCTGGTGC AGATGAAGGA TTTTCGGTTT 
TCTTACAAGT TTAAAATGGC CTGCAAGGAG GACGTGTTGA AGCTTTGCCC AAACATAAAA 
AAGAAGGTGG ACGTGGTGAT CTGCCTGAGC ACGACCGTGC GCAATGACAC TCTGCAGGAA 
GCCAAGGAGC ACAGGGTGTC CCTGAAGTGC CGCAGGCAGC TCCGTGTGGA GGAGCTGGAG 
ATGACGGAGG ACATCCGCTT GGAGCCAGAT CTATACGAAG CCTGCAAGAG TGACATCAAA 
AACTTCTGTT CCGCTGTGCA ATATGGCAAC GCTCAGATTA TCGAATGTCT GAAAGAAAAC 
AAGAAGCAGC TAAGCACCCG CTGCCACCAA AAAGTATTTA AGCTGCAGGA GACAGAGATG 
ATGGACCCAG AGCTAGACTA CACCCTCATG AGGGTCTGCA AGCAGATGAT AAAGAGGTTC 
TGTCCGGAAG CAGATTCTAA AACCATGTTG CAGTGCTTGA AGCAAAATAA AAACAGTGAA 
TTGATGGATC CCAAATGCAA ACAGATGATA ACCAAGCGCC AGATCACCCA GAACACAGAT 
TACCGCTTAA ACCCCATGTT AAGAAAAGCC TGTAAAGCTG ACATTCCTAA ATTCTGTCAC 
GGTATCCTGA CTAAGGCCAA GGATGATTCA GAATTAGAAG GACAAGTCAT CTCTTGCCTG 
AAGCTGAGAT ATGCTGACCA GCGCCTGTCT TCAGACTGTG AAGACCAGAT CCGAATCATT 
ATCCAGGAGT CCGCCCTGGA CTACCGCCTG GATCCTCAGC TCCAGCTGCA CTGCTCAGAC 
GAGATCTCCA GTCTATGTGC TGAAGAAGCA GCAGCCCAAG AGCAGACAGG TCAGGTGGAG 
GAGTGCCTCA AGGTCAACCT GCTCAAGATC AAAACAGAAT TGTGTAAAAA GGAAGTGCTA 
AACATGCTGA AGGAAAGCAA AGCAGACATC TTTGTTGACC CGGTACTTCA TACTGCTTGT 
GCCCTGGACA TTAAACACCA CTGCGCAGCC ATCACCCCTG GCCGCGGGCG TCAAATGTCC 
TGTCTCATGG AAGCACTGGA GGATAAGCGG GTGAGGTTAC AGCCCGAGTG CAAAAAGCGC 
CTCAATGACC GGATTGAGAT GTGGAGTTAC GCAGCAAAGG TGGCCCCAGC AGATGGCTTC 
TCTGATCTTG CCATGCAAGT AATGACGTCT CCATCTAAGA ACTACATTCT CTCTGTGATC 
AGTGGGAGCA TCTGTATATT GTTCCTGATT GGCCTGATGT GTGGACGGAT CACCAAGCGA 
GTGACACGAG AGCTCAAGGA CAGGCTACAA TACAGGTCAG AGACAATGGC TTATAAAGGT 
TTAGTGTGGT CTCAGGATGT GACAGGCAGT CCAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139138.1
CDS	51..3629
Translation	MAACGRVRRMFRLSAALHLLLLFAAGAEKLPGQGVHSQGQGPGANFVSFVGQAGGGGPAGQQLPQLPQSSQLQQQQQQQQQQQQPQPPQPPFPAGGPPARRGGAGAGGGWKLAEEESCREDVTRVCPKHTWSNNLAVLECLQDVRELLWNYKLNLTTDPKFESVAREVCKSTITEIKECADEPVGKGYMVSCLVDHRGNITEYQCHQYITKMTAIIFSDYRLICGFMDDCKNDINILKCGSIRLGEKDAHSQGEVVSCLEKGLVKEAEEREPKIQVSELCKKAILRVAELSSDDFHLDRHLYFACRDDRERFCENTQAGEGRVYKCLFNHKFEESMSEKCREALTTRQKLIAQDYKVSYSLAKSCKSDLKKYRCNVENLPRSREARLSYLLMCLESAVHRGRQVSSECQGEMLDYRRMLMEDFSLSPEIILSCRGEIEHHCSGLHRKGRTLHCLMKVVRGEKGNLGMNCQQALQTLIQETDPGADYRIDRALNEACESVIQTACKHIRSGDPMILSCLMEHLYTEKMVEDCEHRLLELQYFISRDWKLDPVLYRKCQGDASRLCHTHGWNETSEFMPQGAVFSCLYRHAYRTEEQGRRLSRECRAEVQRILHQRAMDVKLDPALQDKCLIDLGKWCSEKTETGQELECLQDHLDDLVVECRDIVGNLTELESEDIQIEALLMRACEPIIQNFCHDVADNQIDSGDLMECLIQNKHQKDMNEKCAIGVTHFQLVQMKDFRFSYKFKMACKEDVLKLCPNIKKKVDVVICLSTTVRNDTLQEAKEHRVSLKCRRQLRVEELEMTEDIRLEPDLYEACKSDIKNFCSAVQYGNAQIIECLKENKKQLSTRCHQKVFKLQETEMMDPELDYTLMRVCKQMIKRFCPEADSKTMLQCLKQNKNSELMDPKCKQMITKRQITQNTDYRLNPMLRKACKADIPKFCHGILTKAKDDSELEGQVISCLKLRYADQRLSSDCEDQIRIIIQESALDYRLDPQLQLHCSDEISSLCAEEAAAQEQTGQVEECLKVNLLKIKTELCKKEVLNMLKESKADIFVDPVLHTACALDIKHHCAAITPGRGRQMSCLMEALEDKRVRLQPECKKRLNDRIEMWSYAAKVAPADGFSDLAMQVMTSPSKNYILSVISGSICILFLIGLMCGRITKRVTRELKDRLQYRSETMAYKGLVWSQDVTGSPA

Target ORF information:

RefSeq Version	NM_001145666.1
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi glycoprotein 1 (GLG1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145666.1

ORF Insert Sequence:

ATGGCGGCGT GTGGACGTGT ACGGAGGATG TTCCGCTTGT CGGCGGCGCT GCATCTGCTG 
CTGCTATTCG CGGCCGGGGC CGAGAAACTC CCCGGCCAGG GCGTCCACAG CCAGGGCCAG 
GGTCCCGGGG CCAACTTTGT GTCCTTCGTA GGGCAGGCCG GAGGCGGCGG CCCGGCGGGT 
CAGCAGCTGC CCCAGCTGCC TCAGTCATCG CAGCTTCAGC AGCAACAGCA GCAGCAGCAA 
CAGCAACAGC AGCCTCAGCC GCCGCAGCCG CCTTTCCCGG CGGGTGGGCC TCCGGCCCGG 
CGGGGAGGAG CGGGGGCTGG TGGGGGCTGG AAGCTGGCGG AGGAAGAGTC CTGCAGGGAG 
GACGTGACCC GCGTGTGCCC TAAGCACACC TGGAGCAACA ACCTGGCGGT GCTCGAGTGC 
CTGCAGGATG TGAGGGAGTT GTTGTGGAAT TATAAGCTGA ACCTAACTAC AGATCCCAAA 
TTTGAATCTG TGGCCAGAGA GGTTTGCAAA TCTACTATAA CAGAGATTAA AGAATGTGCT 
GATGAACCGG TTGGAAAAGG TTACATGGTT TCCTGCTTGG TGGATCACCG AGGCAACATC 
ACTGAGTATC AGTGTCACCA GTACATTACC AAGATGACGG CCATCATTTT TAGTGATTAC 
CGTTTAATCT GTGGCTTCAT GGATGACTGC AAAAATGACA TCAACATTCT GAAATGTGGC 
AGTATTCGGC TTGGAGAAAA GGATGCACAT TCACAAGGTG AGGTGGTATC ATGCTTGGAG 
AAAGGCCTGG TGAAAGAAGC AGAAGAAAGA GAACCCAAGA TTCAAGTTTC TGAACTCTGC 
AAGAAAGCCA TTCTCCGGGT GGCTGAGCTG TCATCGGATG ACTTTCACTT AGACCGGCAT 
TTATATTTTG CTTGCCGAGA TGATCGGGAG CGTTTTTGTG AAAATACACA AGCTGGTGAG 
GGCAGAGTGT ATAAGTGCCT CTTTAACCAT AAATTTGAAG AATCCATGAG TGAAAAGTGT 
CGAGAAGCAC TTACAACCCG CCAAAAGCTG ATTGCCCAGG ATTATAAAGT CAGTTATTCA 
TTGGCCAAAT CCTGTAAAAG TGACTTGAAG AAATACCGGT GCAATGTGGA AAACCTTCCG 
CGATCGCGTG AAGCCAGGCT CTCCTACTTG TTAATGTGCC TGGAGTCAGC TGTACACAGA 
GGGCGACAAG TCAGCAGTGA GTGCCAGGGG GAGATGCTGG ATTACCGACG CATGTTGATG 
GAAGACTTTT CTCTGAGCCC TGAGATCATC CTAAGCTGTC GGGGGGAGAT TGAACACCAT 
TGTTCCGGAT TACATCGAAA AGGGCGGACC CTACACTGTC TGATGAAAGT AGTTCGAGGG 
GAGAAGGGGA ACCTTGGAAT GAACTGCCAG CAGGCGCTTC AAACACTGAT TCAGGAGACT 
GACCCTGGTG CAGATTACCG CATTGATCGA GCTTTGAATG AAGCTTGTGA ATCTGTAATC 
CAGACAGCCT GCAAACATAT AAGATCTGGA GACCCAATGA TCTTGTCGTG CCTGATGGAA 
CATTTATACA CAGAGAAGAT GGTAGAAGAC TGTGAACACC GTCTCTTAGA GCTGCAGTAT 
TTCATCTCCC GGGATTGGAA GCTGGACCCT GTCCTGTACC GCAAGTGCCA GGGAGACGCT 
TCTCGTCTTT GCCACACCCA CGGTTGGAAT GAGACCAGTG AATTTATGCC TCAGGGAGCT 
GTGTTCTCTT GTTTATACAG ACACGCCTAC CGCACTGAGG AACAGGGAAG GAGGCTCTCA 
CGGGAGTGCC GAGCTGAAGT CCAAAGGATC CTACACCAGC GTGCCATGGA TGTCAAGCTG 
GATCCTGCCC TCCAGGATAA GTGCCTGATT GATCTGGGAA AATGGTGCAG TGAGAAAACA 
GAGACTGGAC AGGAGCTGGA GTGCCTTCAG GACCATCTGG ATGACTTGGT GGTGGAGTGT 
AGAGATATAG TTGGCAACCT CACTGAGTTA GAATCAGAGG ATATTCAAAT AGAAGCCTTG 
CTGATGAGAG CCTGTGAGCC CATAATTCAG AACTTCTGCC ACGATGTGGC AGATAACCAG 
ATAGACTCTG GGGACCTGAT GGAGTGTCTG ATACAGAACA AACACCAGAA GGACATGAAC 
GAGAAGTGTG CCATCGGAGT TACCCACTTC CAGCTGGTGC AGATGAAGGA TTTTCGGTTT 
TCTTACAAGT TTAAAATGGC CTGCAAGGAG GACGTGTTGA AGCTTTGCCC AAACATAAAA 
AAGAAGGTGG ACGTGGTGAT CTGCCTGAGC ACGACCGTGC GCAATGACAC TCTGCAGGAA 
GCCAAGGAGC ACAGGGTGTC CCTGAAGTGC CGCAGGCAGC TCCGTGTGGA GGAGCTGGAG 
ATGACGGAGG ACATCCGCTT GGAGCCAGAT CTATACGAAG CCTGCAAGAG TGACATCAAA 
AACTTCTGTT CCGCTGTGCA ATATGGCAAC GCTCAGATTA TCGAATGTCT GAAAGAAAAC 
AAGAAGCAGC TAAGCACCCG CTGCCACCAA AAAGTATTTA AGCTGCAGGA GACAGAGATG 
ATGGACCCAG AGCTAGACTA CACCCTCATG AGGGTCTGCA AGCAGATGAT AAAGAGGTTC 
TGTCCGGAAG CAGATTCTAA AACCATGTTG CAGTGCTTGA AGCAAAATAA AAACAGTGAA 
TTGATGGATC CCAAATGCAA ACAGATGATA ACCAAGCGCC AGATCACCCA GAACACAGAT 
TACCGCTTAA ACCCCATGTT AAGAAAAGCC TGTAAAGCTG ACATTCCTAA ATTCTGTCAC 
GGTATCCTGA CTAAGGCCAA GGATGATTCA GAATTAGAAG GACAAGTCAT CTCTTGCCTG 
AAGCTGAGAT ATGCTGACCA GCGCCTGTCT TCAGACTGTG AAGACCAGAT CCGAATCATT 
ATCCAGGAGT CCGCCCTGGA CTACCGCCTG GATCCTCAGC TCCAGCTGCA CTGCTCAGAC 
GAGATCTCCA GTCTATGTGC TGAAGAAGCA GCAGCCCAAG AGCAGACAGG TCAGGTGGAG 
GAGTGCCTCA AGGTCAACCT GCTCAAGATC AAAACAGAAT TGTGTAAAAA GGAAGTGCTA 
AACATGCTGA AGGAAAGCAA AGCAGACATC TTTGTTGACC CGGTACTTCA TACTGCTTGT 
GCCCTGGACA TTAAACACCA CTGCGCAGCC ATCACCCCTG GCCGCGGGCG TCAAATGTCC 
TGTCTCATGG AAGCACTGGA GGATAAGCGG GTGAGGTTAC AGCCCGAGTG CAAAAAGCGC 
CTCAATGACC GGATTGAGAT GTGGAGTTAC GCAGCAAAGG TGGCCCCAGC AGATGGCTTC 
TCTGATCTTG CCATGCAAGT AATGACGTCT CCATCTAAGA ACTACATTCT CTCTGTGATC 
AGTGGGAGCA TCTGTATATT GTTCCTGATT GGCCTGATGT GTGGACGGAT CACCAAGCGA 
GTGACACGAG AGCTCAAGGA CAGGCTACAA TACAGGTCAG AGACAATGGC TTATAAAGGT 
TTAGTGTGGT CTCAGGATGT GACAGGCAGT CCAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GLG1 ( NM_001145666.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001139138.1 Homo sapiens golgi glycoprotein 1 (GLG1), transcript variant 2, mRNA.

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CloneID	OHu11725
Clone ID Related Accession (Same CDS sequence)	NM_001145666.1 , NM_001145666.2
Accession Version	NM_001145666.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3579bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-28
Organism	Homo sapiens(human)
Product	Golgi apparatus protein 1 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA493962.1, AK304156.1, BC060822.1, AC009053.7 and BX648396.1. On Jun 2, 2019 this sequence version replaced NM_001145666.1. Transcript Variant: This variant (2) lacks an in-frame coding exon, as compared to variant 1. The resulting isoform (2) lacks an internal segment, as compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304156.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCGT GTGGACGTGT ACGGAGGATG TTCCGCTTGT CGGCGGCGCT GCATCTGCTG 
CTGCTATTCG CGGCCGGGGC CGAGAAACTC CCCGGCCAGG GCGTCCACAG CCAGGGCCAG 
GGTCCCGGGG CCAACTTTGT GTCCTTCGTA GGGCAGGCCG GAGGCGGCGG CCCGGCGGGT 
CAGCAGCTGC CCCAGCTGCC TCAGTCATCG CAGCTTCAGC AGCAACAGCA GCAGCAGCAA 
CAGCAACAGC AGCCTCAGCC GCCGCAGCCG CCTTTCCCGG CGGGTGGGCC TCCGGCCCGG 
CGGGGAGGAG CGGGGGCTGG TGGGGGCTGG AAGCTGGCGG AGGAAGAGTC CTGCAGGGAG 
GACGTGACCC GCGTGTGCCC TAAGCACACC TGGAGCAACA ACCTGGCGGT GCTCGAGTGC 
CTGCAGGATG TGAGGGAGTT GTTGTGGAAT TATAAGCTGA ACCTAACTAC AGATCCCAAA 
TTTGAATCTG TGGCCAGAGA GGTTTGCAAA TCTACTATAA CAGAGATTAA AGAATGTGCT 
GATGAACCGG TTGGAAAAGG TTACATGGTT TCCTGCTTGG TGGATCACCG AGGCAACATC 
ACTGAGTATC AGTGTCACCA GTACATTACC AAGATGACGG CCATCATTTT TAGTGATTAC 
CGTTTAATCT GTGGCTTCAT GGATGACTGC AAAAATGACA TCAACATTCT GAAATGTGGC 
AGTATTCGGC TTGGAGAAAA GGATGCACAT TCACAAGGTG AGGTGGTATC ATGCTTGGAG 
AAAGGCCTGG TGAAAGAAGC AGAAGAAAGA GAACCCAAGA TTCAAGTTTC TGAACTCTGC 
AAGAAAGCCA TTCTCCGGGT GGCTGAGCTG TCATCGGATG ACTTTCACTT AGACCGGCAT 
TTATATTTTG CTTGCCGAGA TGATCGGGAG CGTTTTTGTG AAAATACACA AGCTGGTGAG 
GGCAGAGTGT ATAAGTGCCT CTTTAACCAT AAATTTGAAG AATCCATGAG TGAAAAGTGT 
CGAGAAGCAC TTACAACCCG CCAAAAGCTG ATTGCCCAGG ATTATAAAGT CAGTTATTCA 
TTGGCCAAAT CCTGTAAAAG TGACTTGAAG AAATACCGGT GCAATGTGGA AAACCTTCCG 
CGATCGCGTG AAGCCAGGCT CTCCTACTTG TTAATGTGCC TGGAGTCAGC TGTACACAGA 
GGGCGACAAG TCAGCAGTGA GTGCCAGGGG GAGATGCTGG ATTACCGACG CATGTTGATG 
GAAGACTTTT CTCTGAGCCC TGAGATCATC CTAAGCTGTC GGGGGGAGAT TGAACACCAT 
TGTTCCGGAT TACATCGAAA AGGGCGGACC CTACACTGTC TGATGAAAGT AGTTCGAGGG 
GAGAAGGGGA ACCTTGGAAT GAACTGCCAG CAGGCGCTTC AAACACTGAT TCAGGAGACT 
GACCCTGGTG CAGATTACCG CATTGATCGA GCTTTGAATG AAGCTTGTGA ATCTGTAATC 
CAGACAGCCT GCAAACATAT AAGATCTGGA GACCCAATGA TCTTGTCGTG CCTGATGGAA 
CATTTATACA CAGAGAAGAT GGTAGAAGAC TGTGAACACC GTCTCTTAGA GCTGCAGTAT 
TTCATCTCCC GGGATTGGAA GCTGGACCCT GTCCTGTACC GCAAGTGCCA GGGAGACGCT 
TCTCGTCTTT GCCACACCCA CGGTTGGAAT GAGACCAGTG AATTTATGCC TCAGGGAGCT 
GTGTTCTCTT GTTTATACAG ACACGCCTAC CGCACTGAGG AACAGGGAAG GAGGCTCTCA 
CGGGAGTGCC GAGCTGAAGT CCAAAGGATC CTACACCAGC GTGCCATGGA TGTCAAGCTG 
GATCCTGCCC TCCAGGATAA GTGCCTGATT GATCTGGGAA AATGGTGCAG TGAGAAAACA 
GAGACTGGAC AGGAGCTGGA GTGCCTTCAG GACCATCTGG ATGACTTGGT GGTGGAGTGT 
AGAGATATAG TTGGCAACCT CACTGAGTTA GAATCAGAGG ATATTCAAAT AGAAGCCTTG 
CTGATGAGAG CCTGTGAGCC CATAATTCAG AACTTCTGCC ACGATGTGGC AGATAACCAG 
ATAGACTCTG GGGACCTGAT GGAGTGTCTG ATACAGAACA AACACCAGAA GGACATGAAC 
GAGAAGTGTG CCATCGGAGT TACCCACTTC CAGCTGGTGC AGATGAAGGA TTTTCGGTTT 
TCTTACAAGT TTAAAATGGC CTGCAAGGAG GACGTGTTGA AGCTTTGCCC AAACATAAAA 
AAGAAGGTGG ACGTGGTGAT CTGCCTGAGC ACGACCGTGC GCAATGACAC TCTGCAGGAA 
GCCAAGGAGC ACAGGGTGTC CCTGAAGTGC CGCAGGCAGC TCCGTGTGGA GGAGCTGGAG 
ATGACGGAGG ACATCCGCTT GGAGCCAGAT CTATACGAAG CCTGCAAGAG TGACATCAAA 
AACTTCTGTT CCGCTGTGCA ATATGGCAAC GCTCAGATTA TCGAATGTCT GAAAGAAAAC 
AAGAAGCAGC TAAGCACCCG CTGCCACCAA AAAGTATTTA AGCTGCAGGA GACAGAGATG 
ATGGACCCAG AGCTAGACTA CACCCTCATG AGGGTCTGCA AGCAGATGAT AAAGAGGTTC 
TGTCCGGAAG CAGATTCTAA AACCATGTTG CAGTGCTTGA AGCAAAATAA AAACAGTGAA 
TTGATGGATC CCAAATGCAA ACAGATGATA ACCAAGCGCC AGATCACCCA GAACACAGAT 
TACCGCTTAA ACCCCATGTT AAGAAAAGCC TGTAAAGCTG ACATTCCTAA ATTCTGTCAC 
GGTATCCTGA CTAAGGCCAA GGATGATTCA GAATTAGAAG GACAAGTCAT CTCTTGCCTG 
AAGCTGAGAT ATGCTGACCA GCGCCTGTCT TCAGACTGTG AAGACCAGAT CCGAATCATT 
ATCCAGGAGT CCGCCCTGGA CTACCGCCTG GATCCTCAGC TCCAGCTGCA CTGCTCAGAC 
GAGATCTCCA GTCTATGTGC TGAAGAAGCA GCAGCCCAAG AGCAGACAGG TCAGGTGGAG 
GAGTGCCTCA AGGTCAACCT GCTCAAGATC AAAACAGAAT TGTGTAAAAA GGAAGTGCTA 
AACATGCTGA AGGAAAGCAA AGCAGACATC TTTGTTGACC CGGTACTTCA TACTGCTTGT 
GCCCTGGACA TTAAACACCA CTGCGCAGCC ATCACCCCTG GCCGCGGGCG TCAAATGTCC 
TGTCTCATGG AAGCACTGGA GGATAAGCGG GTGAGGTTAC AGCCCGAGTG CAAAAAGCGC 
CTCAATGACC GGATTGAGAT GTGGAGTTAC GCAGCAAAGG TGGCCCCAGC AGATGGCTTC 
TCTGATCTTG CCATGCAAGT AATGACGTCT CCATCTAAGA ACTACATTCT CTCTGTGATC 
AGTGGGAGCA TCTGTATATT GTTCCTGATT GGCCTGATGT GTGGACGGAT CACCAAGCGA 
GTGACACGAG AGCTCAAGGA CAGGCTACAA TACAGGTCAG AGACAATGGC TTATAAAGGT 
TTAGTGTGGT CTCAGGATGT GACAGGCAGT CCAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139138.1
CDS	21..3599
Translation	MAACGRVRRMFRLSAALHLLLLFAAGAEKLPGQGVHSQGQGPGANFVSFVGQAGGGGPAGQQLPQLPQSSQLQQQQQQQQQQQQPQPPQPPFPAGGPPARRGGAGAGGGWKLAEEESCREDVTRVCPKHTWSNNLAVLECLQDVRELLWNYKLNLTTDPKFESVAREVCKSTITEIKECADEPVGKGYMVSCLVDHRGNITEYQCHQYITKMTAIIFSDYRLICGFMDDCKNDINILKCGSIRLGEKDAHSQGEVVSCLEKGLVKEAEEREPKIQVSELCKKAILRVAELSSDDFHLDRHLYFACRDDRERFCENTQAGEGRVYKCLFNHKFEESMSEKCREALTTRQKLIAQDYKVSYSLAKSCKSDLKKYRCNVENLPRSREARLSYLLMCLESAVHRGRQVSSECQGEMLDYRRMLMEDFSLSPEIILSCRGEIEHHCSGLHRKGRTLHCLMKVVRGEKGNLGMNCQQALQTLIQETDPGADYRIDRALNEACESVIQTACKHIRSGDPMILSCLMEHLYTEKMVEDCEHRLLELQYFISRDWKLDPVLYRKCQGDASRLCHTHGWNETSEFMPQGAVFSCLYRHAYRTEEQGRRLSRECRAEVQRILHQRAMDVKLDPALQDKCLIDLGKWCSEKTETGQELECLQDHLDDLVVECRDIVGNLTELESEDIQIEALLMRACEPIIQNFCHDVADNQIDSGDLMECLIQNKHQKDMNEKCAIGVTHFQLVQMKDFRFSYKFKMACKEDVLKLCPNIKKKVDVVICLSTTVRNDTLQEAKEHRVSLKCRRQLRVEELEMTEDIRLEPDLYEACKSDIKNFCSAVQYGNAQIIECLKENKKQLSTRCHQKVFKLQETEMMDPELDYTLMRVCKQMIKRFCPEADSKTMLQCLKQNKNSELMDPKCKQMITKRQITQNTDYRLNPMLRKACKADIPKFCHGILTKAKDDSELEGQVISCLKLRYADQRLSSDCEDQIRIIIQESALDYRLDPQLQLHCSDEISSLCAEEAAAQEQTGQVEECLKVNLLKIKTELCKKEVLNMLKESKADIFVDPVLHTACALDIKHHCAAITPGRGRQMSCLMEALEDKRVRLQPECKKRLNDRIEMWSYAAKVAPADGFSDLAMQVMTSPSKNYILSVISGSICILFLIGLMCGRITKRVTRELKDRLQYRSETMAYKGLVWSQDVTGSPA

Target ORF information:

RefSeq Version	NM_001145666.2
Organism	Homo sapiens(human)
Definition	Homo sapiens golgi glycoprotein 1 (GLG1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145666.2

ORF Insert Sequence:

ATGGCGGCGT GTGGACGTGT ACGGAGGATG TTCCGCTTGT CGGCGGCGCT GCATCTGCTG 
CTGCTATTCG CGGCCGGGGC CGAGAAACTC CCCGGCCAGG GCGTCCACAG CCAGGGCCAG 
GGTCCCGGGG CCAACTTTGT GTCCTTCGTA GGGCAGGCCG GAGGCGGCGG CCCGGCGGGT 
CAGCAGCTGC CCCAGCTGCC TCAGTCATCG CAGCTTCAGC AGCAACAGCA GCAGCAGCAA 
CAGCAACAGC AGCCTCAGCC GCCGCAGCCG CCTTTCCCGG CGGGTGGGCC TCCGGCCCGG 
CGGGGAGGAG CGGGGGCTGG TGGGGGCTGG AAGCTGGCGG AGGAAGAGTC CTGCAGGGAG 
GACGTGACCC GCGTGTGCCC TAAGCACACC TGGAGCAACA ACCTGGCGGT GCTCGAGTGC 
CTGCAGGATG TGAGGGAGTT GTTGTGGAAT TATAAGCTGA ACCTAACTAC AGATCCCAAA 
TTTGAATCTG TGGCCAGAGA GGTTTGCAAA TCTACTATAA CAGAGATTAA AGAATGTGCT 
GATGAACCGG TTGGAAAAGG TTACATGGTT TCCTGCTTGG TGGATCACCG AGGCAACATC 
ACTGAGTATC AGTGTCACCA GTACATTACC AAGATGACGG CCATCATTTT TAGTGATTAC 
CGTTTAATCT GTGGCTTCAT GGATGACTGC AAAAATGACA TCAACATTCT GAAATGTGGC 
AGTATTCGGC TTGGAGAAAA GGATGCACAT TCACAAGGTG AGGTGGTATC ATGCTTGGAG 
AAAGGCCTGG TGAAAGAAGC AGAAGAAAGA GAACCCAAGA TTCAAGTTTC TGAACTCTGC 
AAGAAAGCCA TTCTCCGGGT GGCTGAGCTG TCATCGGATG ACTTTCACTT AGACCGGCAT 
TTATATTTTG CTTGCCGAGA TGATCGGGAG CGTTTTTGTG AAAATACACA AGCTGGTGAG 
GGCAGAGTGT ATAAGTGCCT CTTTAACCAT AAATTTGAAG AATCCATGAG TGAAAAGTGT 
CGAGAAGCAC TTACAACCCG CCAAAAGCTG ATTGCCCAGG ATTATAAAGT CAGTTATTCA 
TTGGCCAAAT CCTGTAAAAG TGACTTGAAG AAATACCGGT GCAATGTGGA AAACCTTCCG 
CGATCGCGTG AAGCCAGGCT CTCCTACTTG TTAATGTGCC TGGAGTCAGC TGTACACAGA 
GGGCGACAAG TCAGCAGTGA GTGCCAGGGG GAGATGCTGG ATTACCGACG CATGTTGATG 
GAAGACTTTT CTCTGAGCCC TGAGATCATC CTAAGCTGTC GGGGGGAGAT TGAACACCAT 
TGTTCCGGAT TACATCGAAA AGGGCGGACC CTACACTGTC TGATGAAAGT AGTTCGAGGG 
GAGAAGGGGA ACCTTGGAAT GAACTGCCAG CAGGCGCTTC AAACACTGAT TCAGGAGACT 
GACCCTGGTG CAGATTACCG CATTGATCGA GCTTTGAATG AAGCTTGTGA ATCTGTAATC 
CAGACAGCCT GCAAACATAT AAGATCTGGA GACCCAATGA TCTTGTCGTG CCTGATGGAA 
CATTTATACA CAGAGAAGAT GGTAGAAGAC TGTGAACACC GTCTCTTAGA GCTGCAGTAT 
TTCATCTCCC GGGATTGGAA GCTGGACCCT GTCCTGTACC GCAAGTGCCA GGGAGACGCT 
TCTCGTCTTT GCCACACCCA CGGTTGGAAT GAGACCAGTG AATTTATGCC TCAGGGAGCT 
GTGTTCTCTT GTTTATACAG ACACGCCTAC CGCACTGAGG AACAGGGAAG GAGGCTCTCA 
CGGGAGTGCC GAGCTGAAGT CCAAAGGATC CTACACCAGC GTGCCATGGA TGTCAAGCTG 
GATCCTGCCC TCCAGGATAA GTGCCTGATT GATCTGGGAA AATGGTGCAG TGAGAAAACA 
GAGACTGGAC AGGAGCTGGA GTGCCTTCAG GACCATCTGG ATGACTTGGT GGTGGAGTGT 
AGAGATATAG TTGGCAACCT CACTGAGTTA GAATCAGAGG ATATTCAAAT AGAAGCCTTG 
CTGATGAGAG CCTGTGAGCC CATAATTCAG AACTTCTGCC ACGATGTGGC AGATAACCAG 
ATAGACTCTG GGGACCTGAT GGAGTGTCTG ATACAGAACA AACACCAGAA GGACATGAAC 
GAGAAGTGTG CCATCGGAGT TACCCACTTC CAGCTGGTGC AGATGAAGGA TTTTCGGTTT 
TCTTACAAGT TTAAAATGGC CTGCAAGGAG GACGTGTTGA AGCTTTGCCC AAACATAAAA 
AAGAAGGTGG ACGTGGTGAT CTGCCTGAGC ACGACCGTGC GCAATGACAC TCTGCAGGAA 
GCCAAGGAGC ACAGGGTGTC CCTGAAGTGC CGCAGGCAGC TCCGTGTGGA GGAGCTGGAG 
ATGACGGAGG ACATCCGCTT GGAGCCAGAT CTATACGAAG CCTGCAAGAG TGACATCAAA 
AACTTCTGTT CCGCTGTGCA ATATGGCAAC GCTCAGATTA TCGAATGTCT GAAAGAAAAC 
AAGAAGCAGC TAAGCACCCG CTGCCACCAA AAAGTATTTA AGCTGCAGGA GACAGAGATG 
ATGGACCCAG AGCTAGACTA CACCCTCATG AGGGTCTGCA AGCAGATGAT AAAGAGGTTC 
TGTCCGGAAG CAGATTCTAA AACCATGTTG CAGTGCTTGA AGCAAAATAA AAACAGTGAA 
TTGATGGATC CCAAATGCAA ACAGATGATA ACCAAGCGCC AGATCACCCA GAACACAGAT 
TACCGCTTAA ACCCCATGTT AAGAAAAGCC TGTAAAGCTG ACATTCCTAA ATTCTGTCAC 
GGTATCCTGA CTAAGGCCAA GGATGATTCA GAATTAGAAG GACAAGTCAT CTCTTGCCTG 
AAGCTGAGAT ATGCTGACCA GCGCCTGTCT TCAGACTGTG AAGACCAGAT CCGAATCATT 
ATCCAGGAGT CCGCCCTGGA CTACCGCCTG GATCCTCAGC TCCAGCTGCA CTGCTCAGAC 
GAGATCTCCA GTCTATGTGC TGAAGAAGCA GCAGCCCAAG AGCAGACAGG TCAGGTGGAG 
GAGTGCCTCA AGGTCAACCT GCTCAAGATC AAAACAGAAT TGTGTAAAAA GGAAGTGCTA 
AACATGCTGA AGGAAAGCAA AGCAGACATC TTTGTTGACC CGGTACTTCA TACTGCTTGT 
GCCCTGGACA TTAAACACCA CTGCGCAGCC ATCACCCCTG GCCGCGGGCG TCAAATGTCC 
TGTCTCATGG AAGCACTGGA GGATAAGCGG GTGAGGTTAC AGCCCGAGTG CAAAAAGCGC 
CTCAATGACC GGATTGAGAT GTGGAGTTAC GCAGCAAAGG TGGCCCCAGC AGATGGCTTC 
TCTGATCTTG CCATGCAAGT AATGACGTCT CCATCTAAGA ACTACATTCT CTCTGTGATC 
AGTGGGAGCA TCTGTATATT GTTCCTGATT GGCCTGATGT GTGGACGGAT CACCAAGCGA 
GTGACACGAG AGCTCAAGGA CAGGCTACAA TACAGGTCAG AGACAATGGC TTATAAAGGT 
TTAGTGTGGT CTCAGGATGT GACAGGCAGT CCAGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Polysialylation and lipopolysaccharide-induced shedding of E-selectin ligand-1 and neuropilin-2 by microglia and THP-1 macrophages.
Glia64(8)1314-30(2016 Aug)
Werneburg S,Buettner FF,Erben L,Mathews M,Neumann H,M?hlenhoff M,Hildebrandt H

E-selectin ligand-1 (ESL-1) is a novel adiponectin binding protein on cell adhesion.
Biochemical and biophysical research communications470(2)425-430(2016 Feb)
Yamamoto H,Kuroda N,Uekita H,Kochi I,Matsumoto A,Niinaga R,Funahashi T,Shimomura I,Kihara S

E-selectin ligand-1 controls circulating prostate cancer cell rolling/adhesion and metastasis.
Oncotarget5(23)12097-110(2014 Dec)
Yasmin-Karim S,King MR,Messing EM,Lee YF

Meta-analysis identifies four new loci associated with testicular germ cell tumor.
Nature genetics45(6)680-5(2013 Jun)
Chung CC,Kanetsky PA,Wang Z,Hildebrandt MA,Koster R,Skotheim RI,Kratz CP,Turnbull C,Cortessis VK,Bakken AC,Bishop DT,Cook MB,Erickson RL,Foss? SD,Jacobs KB,Korde LA,Kraggerud SM,Lothe RA,Loud JT,Rahman N,Skinner EC,Thomas DC,Wu X,Yeager M,Schumacher FR,Greene MH,Schwartz SM,McGlynn KA,Chanock SJ,Nathanson KL

Leukocyte ligands for endothelial selectins: specialized glycoconjugates that mediate rolling and signaling under flow.
Blood118(26)6743-51(2011 Dec)
Zarbock A,Ley K,McEver RP,Hidalgo A

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GLG1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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