Ptafr cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ptafr
Entrez Gene ID	19204
Full Name	platelet-activating factor receptor
Synonyms	PAFR
General protein information	Preferred Names platelet-activating factor receptor Names platelet-activating factor receptor PAF receptor
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4 D2.3\|4 65.56 cM
Summary	This gene encodes a member of the G-protein coupled receptor 1 family of proteins. Binding of the encoded protein to its phospholipid ligand, platelet-activating factor (PAF), may regulate the inflammatory response and the perception of pain. Homozygous knockout mice for this gene exhibit impaired anaphylactic response, resistance to bacterial infection, and reduced pain-related behavior. [provided by RefSeq, Aug 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001081211.2	NP_001074680.1	platelet-activating factor receptor

Pathways from BioSystems

Homologies

	PTAFR	XP_001112942.2
Canis lupus familiaris (dog)	PTAFR	XP_005617766.1
Rattus norvegicus (Norway rat)	Ptafr	NP_445773.1
Xenopus tropicalis (tropical clawed frog)	ptafr	NP_001008003.1
Homo sapiens (human)	PTAFR	NP_000943.1
Pan troglodytes (chimpanzee)	PTAFR	XP_001151906.1
Bos taurus (cattle)	PTAFR	NP_001035628.1
Mus musculus (house mouse)	Ptafr	NP_001074680.1
Danio rerio (zebrafish)	LOC100002627	XP_001342372.1
Gallus gallus (chicken)	PTAFR	XP_004947758.1

Gene Ontology
Bibliography

Related articles in PubMed

PAFR-deficiency alleviates myocardial ischemia/reperfusion injury in mice via suppressing inflammation, oxidative stress and apoptosis.
Wang EW, Han YY, Jia XS
Biochemical and biophysical research communications495(4)2475-2481(2018 Jan)

CD36 and Platelet-Activating Factor Receptor Promote House Dust Mite Allergy Development.
Patel PS, Kearney JF
Journal of immunology (Baltimore, Md. : 1950)199(3)1184-1195(2017 Aug)

Loss of PAFR prevents neuroinflammation and brain dysfunction after traumatic brain injury.
Yin XJ, Chen ZY, Zhu XN, Hu JJ
Scientific reports740614(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results show that CD36 and Platelet-Activating Factor Receptor are important mediators of house dust mites (HDM) allergy development and that inhibiting HDM engagement with phosphorylcholine receptors in the lung protects against allergic airway disease.
Title: CD36 and Platelet-Activating Factor Receptor Promote House Dust Mite Allergy Development.

PAFR null mutant mice showed a better functional recovery in grip and rotarod performances than wild-type mice.
Title: Attenuated Reactive Gliosis and Enhanced Functional Recovery Following Spinal Cord Injury in Null Mutant Mice of Platelet-Activating Factor Receptor.

In the absence of PAFR signalling, monocytes and macrophages acquire a pro-inflammatory phenotype, resulting in adipose tissue inflammation and metabolic dysfunction.
Title: PAFR in adipose tissue macrophages is associated with anti-inflammatory phenotype and metabolic homoeostasis.

Study demonstrated that PAFR has a compelling involvement in Brucella abortus uptake as a promoter of phagocytosis, which is associated with JAK2 signaling activation.
Title: Influence of platelet-activating factor receptor (PAFR) on Brucella abortus infection: implications for manipulating the phagocytic strategy of B. abortus.

Data (including data from studies in knockout mice) suggest that signal transduction via platelet-activating factor (PAF) and PAF receptor is involved in regulation of lipid metabolism/inflammation in liver of mice on high-refined carbohydrate diet.
Title: Platelet-activating factor modulates fat storage in the liver induced by a high-refined carbohydrate-containing diet.

The following Ptafr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ptafr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22094	NM_001081211.2 Latest version!	Mus musculus platelet-activating factor receptor (Ptafr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ptafr ( NM_001081211.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001074680.1 Mus musculus platelet-activating factor receptor (Ptafr), mRNA.

Price & Availability↑

CloneID	OMu22094
Clone ID Related Accession (Same CDS sequence)	NM_001081211.2
Accession Version	NM_001081211.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-14
Organism	Mus musculus(house mouse)
Product	platelet-activating factor receptor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY194666.1, AK032547.1, BY203963.1, CR931794.3 and AA960103.1. On Aug 19, 2015 this sequence version replaced NM_001081211.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK032547.1, BY203963.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCACA ATGGCTCCTT TCGTGTGGAT TCTGAGTTTC GATACACGCT CTTTCCGATT 
GTTTACAGTG TCATCTTTAT ACTGGGGGTG GTTGCCAACG GCTATGTGCT ATGGGTCTTT 
GCTAACTTGT ACCCTTCCAA GAAACTAAAT GAGATAAAGA TCTTTATGGT GAATCTCACT 
ATGGCTGACC TGCTCTTCCT GATCACCCTC CCACTGTGGA TTGTCTACTA CTACAACGAG 
GGCGACTGGA TTCTACCCAA CTTCCTGTGC AACGTGGCTG GCTGCCTCTT CTTCATCAAT 
ACCTACTGCA GTGTGGCCTT TTTGGGTGTC ATCACTTATA ACCGCTACCA GGCAGTAGCC 
TATCCCATCA AGACTGCACA GGCCACCACC CGCAAGCGTG GCATCTCTTT GTCCCTGATC 
ATTTGGGTAT CCATTGTGGC TACTGCATCC TATTTCCTGG CCACAGACTC CACCAACCTA 
GTGCCCAATA AGGATGGCTC AGGCAACATC ACCCGCTGCT TTGAGCATTA TGAGCCATAC 
AGTGTGCCCA TCCTTGTTGT TCATGTCTTC ATCGCCTTCT GCTTCTTCCT CGTCTTCTTC 
CTTATCTTCT ACTGCAACTT GGTCATCATC CACACGCTGC TCACGCAGCC CATGAGGCAG 
CAGCGCAAAG CGGGGGTGAA GCGGAGGGCG CTGTGGATGG TCTGCACGGT CTTGGCGGTA 
TTCATCATCT GTTTTGTGCC CCATCACGTG GTCCAGCTGC CCTGGACCCT AGCAGAGTTG 
GGCTACCAGA CCAACTTCCA TCAGGCTATT AATGATGCCC ATCAAATCAC CCTCTGCCTC 
CTCAGCACCA ACTGTGTCTT AGATCCCGTT ATCTATTGCT TTCTTACCAA GAAGTTCCGA 
AAGCACCTCA GTGAGAAGTT TTACAGCATG CGCAGTAGCC GGAAGTGCTC CAGAGCCACG 
AGTGACACGT GCACCGAGGT GATAGTGCCA GCCAACCAGA CTCCTATTGT GTCGCTGAAA 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074680.1
CDS	255..1280
Translation	MEHNGSFRVDSEFRYTLFPIVYSVIFILGVVANGYVLWVFANLYPSKKLNEIKIFMVNLTMADLLFLITLPLWIVYYYNEGDWILPNFLCNVAGCLFFINTYCSVAFLGVITYNRYQAVAYPIKTAQATTRKRGISLSLIIWVSIVATASYFLATDSTNLVPNKDGSGNITRCFEHYEPYSVPILVVHVFIAFCFFLVFFLIFYCNLVIIHTLLTQPMRQQRKAGVKRRALWMVCTVLAVFIICFVPHHVVQLPWTLAELGYQTNFHQAINDAHQITLCLLSTNCVLDPVIYCFLTKKFRKHLSEKFYSMRSSRKCSRATSDTCTEVIVPANQTPIVSLKN

Target ORF information:

RefSeq Version	NM_001081211.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus platelet-activating factor receptor (Ptafr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081211.2

ORF Insert Sequence:

ATGGAGCACA ATGGCTCCTT TCGTGTGGAT TCTGAGTTTC GATACACGCT CTTTCCGATT 
GTTTACAGTG TCATCTTTAT ACTGGGGGTG GTTGCCAACG GCTATGTGCT ATGGGTCTTT 
GCTAACTTGT ACCCTTCCAA GAAACTAAAT GAGATAAAGA TCTTTATGGT GAATCTCACT 
ATGGCTGACC TGCTCTTCCT GATCACCCTC CCACTGTGGA TTGTCTACTA CTACAACGAG 
GGCGACTGGA TTCTACCCAA CTTCCTGTGC AACGTGGCTG GCTGCCTCTT CTTCATCAAT 
ACCTACTGCA GTGTGGCCTT TTTGGGTGTC ATCACTTATA ACCGCTACCA GGCAGTAGCC 
TATCCCATCA AGACTGCACA GGCCACCACC CGCAAGCGTG GCATCTCTTT GTCCCTGATC 
ATTTGGGTAT CCATTGTGGC TACTGCATCC TATTTCCTGG CCACAGACTC CACCAACCTA 
GTGCCCAATA AGGATGGCTC AGGCAACATC ACCCGCTGCT TTGAGCATTA TGAGCCATAC 
AGTGTGCCCA TCCTTGTTGT TCATGTCTTC ATCGCCTTCT GCTTCTTCCT CGTCTTCTTC 
CTTATCTTCT ACTGCAACTT GGTCATCATC CACACGCTGC TCACGCAGCC CATGAGGCAG 
CAGCGCAAAG CGGGGGTGAA GCGGAGGGCG CTGTGGATGG TCTGCACGGT CTTGGCGGTA 
TTCATCATCT GTTTTGTGCC CCATCACGTG GTCCAGCTGC CCTGGACCCT AGCAGAGTTG 
GGCTACCAGA CCAACTTCCA TCAGGCTATT AATGATGCCC ATCAAATCAC CCTCTGCCTC 
CTCAGCACCA ACTGTGTCTT AGATCCCGTT ATCTATTGCT TTCTTACCAA GAAGTTCCGA 
AAGCACCTCA GTGAGAAGTT TTACAGCATG CGCAGTAGCC GGAAGTGCTC CAGAGCCACG 
AGTGACACGT GCACCGAGGT GATAGTGCCA GCCAACCAGA CTCCTATTGT GTCGCTGAAA 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PAFR-deficiency alleviates myocardial ischemia/reperfusion injury in mice via suppressing inflammation, oxidative stress and apoptosis.
Biochemical and biophysical research communications495(4)2475-2481(2018 Jan)
Wang EW,Han YY,Jia XS

CD36 and Platelet-Activating Factor Receptor Promote House Dust Mite Allergy Development.
Journal of immunology (Baltimore, Md. : 1950)199(3)1184-1195(2017 Aug)
Patel PS,Kearney JF

Loss of PAFR prevents neuroinflammation and brain dysfunction after traumatic brain injury.
Scientific reports740614(2017 Jan)
Yin XJ,Chen ZY,Zhu XN,Hu JJ

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Ptafr cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報