PTAFR cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PTAFR
Entrez Gene ID	5724
Full Name	platelet activating factor receptor
Synonyms	PAFR
General protein information	Preferred Names platelet activating factor receptor Names platelet-activating factor receptor PAF-R
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p35.3
Summary	This gene encodes a seven-transmembrane G-protein-coupled receptor for platelet-activating factor (PAF) that localizes to lipid rafts and/or caveolae in the cell membrane. PAF (1-0-alkyl-2-acetyl-sn-glycero-3-phosphorylcholine) is a phospholipid that plays a significant role in oncogenic transformation, tumor growth, angiogenesis, metastasis, and pro-inflammatory processes. Binding of PAF to the PAF-receptor (PAFR) stimulates numerous signal transduction pathways including phospholipase C, D, A2, mitogen-activated protein kinases (MAPKs), and the phosphatidylinositol-calcium second messenger system. Following PAFR activation, cells become rapidly desensitized and this refractory state is dependent on PAFR phosphorylation, internalization, and down-regulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011].
Disorder MIM:	173393

mRNA and Protein(s)

mRNA	Protein	Name
NM_001164723.2	NP_001158195.1	platelet-activating factor receptor
NM_001164723.3	NP_001158195.1	platelet-activating factor receptor
NM_001164722.2	NP_001158194.1	platelet-activating factor receptor
NM_001164722.3	NP_001158194.1	platelet-activating factor receptor
NM_001164721.1	NP_001158193.1	platelet-activating factor receptor
NM_000952.4	NP_000943.1	platelet-activating factor receptor
NM_000952.5	NP_000943.1	platelet-activating factor receptor

Pathways from BioSystems

Homologies

	PTAFR	XP_001112942.2
Canis lupus familiaris (dog)	PTAFR	XP_005617766.1
Rattus norvegicus (Norway rat)	Ptafr	NP_445773.1
Xenopus tropicalis (tropical clawed frog)	ptafr	NP_001008003.1
Homo sapiens (human)	PTAFR	NP_000943.1
Pan troglodytes (chimpanzee)	PTAFR	XP_001151906.1
Bos taurus (cattle)	PTAFR	NP_001035628.1
Mus musculus (house mouse)	Ptafr	NP_001074680.1
Danio rerio (zebrafish)	LOC100002627	XP_001342372.1
Gallus gallus (chicken)	PTAFR	XP_004947758.1

Gene Ontology
Bibliography

Related articles in PubMed

Association of platelet-activating factor receptor gene rs5938 (G/T) and rs313152 (T/C) polymorphisms with coronary heart disease and blood stasis syndrome in a Chinese Han population.
Zheng GH, Xiong SQ, Chen HY, Mei LJ, Wang T
Chinese journal of integrative medicine23(12)893-900(2017 Dec)

Weighted gene co-expression network analysis of gene modules for the prognosis of esophageal cancer.
Zhang C, Sun Q
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban37(3)319-325(2017 Jun)

Systematic protein-protein interaction mapping for clinically relevant human GPCRs.
Sokolina K, Kittanakom S, Snider J, Kotlyar M, Maurice P, Gand?a J, Benleulmi-Chaachoua A, Tadagaki K, Oishi A, Wong V, Malty RH, Deineko V, Aoki H, Amin S, Yao Z, Morat? X, Otasek D, Kobayashi H, Menendez J, Auerbach D, Angers S, Pr?ulj N, Bouvier M, Babu M, Ciruela F, Jockers R, Jurisica I, Stagljar I
Molecular systems biology13(3)918(2017 03)

Pneumococcal infection of respiratory cells exposed to welding fumes; Role of oxidative stress and HIF-1 alpha.
Grigg J, Miyashita L, Suri R
PloS one12(3)e0173569(2017)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we demonstrate that PAFR inhibition can selectively enhances the sensitivity of radiotherapy in prostate cancer cells with elevated PAFR expression after irradiation exposure
Title: PAFR selectively mediates radioresistance and irradiation-induced autophagy suppression in prostate cancer cells.

PAFR overexpression is associated with pneumococcal infection.
Title: Pneumococcal infection of respiratory cells exposed to welding fumes; Role of oxidative stress and HIF-1 alpha.

PAF stimulation dose-dependently promoted the invasion, migration and growth of prostate cancer cells in vitro, while knockdow our findings demonstrate that PAFR can activate ERK1/2 pathway, and subsequently increase MMP-3 expression and decrease E-cadherin expression
Title: Platelet-activating factor receptor activation promotes prostate cancer cell growth, invasion and metastasis via ERK1/2 pathway.

WEB-2086 represents an innovative class of candidate drugs for inhibiting PAFr-dependent lung infections caused by the main bacterial drivers of smoking-related COPD.
Title: An antagonist of the platelet-activating factor receptor inhibits adherence of both nontypeable Haemophilus influenzae and Streptococcus pneumoniae to cultured human bronchial epithelial cells exposed to cigarette smoke.

PAFR is overexpressed in NSCLC as well as in breast, colorectal, and gastric carcinomas. PAFR expression correlates with clinical stages, survival time, and distant metastasis. PAF/PAFR signaling also upregulated IL6 expression and STAT3 activation.
Title: Feed-Forward Reciprocal Activation of PAFR and STAT3 Regulates Epithelial-Mesenchymal Transition in Non-Small Cell Lung Cancer.

The following PTAFR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PTAFR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu15818	NM_001164722.3 Latest version!	Homo sapiens platelet activating factor receptor (PTAFR), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu15818	NM_001164721.1 Latest version!	Homo sapiens platelet activating factor receptor (PTAFR), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu15818	NM_001164723.3 Latest version!	Homo sapiens platelet activating factor receptor (PTAFR), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu15818	NM_000952.5 Latest version!	Homo sapiens platelet activating factor receptor (PTAFR), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu15818	NM_001164723.2	Homo sapiens platelet activating factor receptor (PTAFR), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu15818	NM_001164722.2	Homo sapiens platelet activating factor receptor (PTAFR), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu15818	NM_000952.4	Homo sapiens platelet activating factor receptor (PTAFR), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PTAFR ( NM_001164723.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001158195.1 Homo sapiens platelet activating factor receptor (PTAFR), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu15818
Clone ID Related Accession (Same CDS sequence)	NM_001164723.2 , NM_001164723.3 , NM_001164722.2 , NM_001164722.3 , NM_001164721.1 , NM_000952.4 , NM_000952.5
Accession Version	NM_001164723.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	platelet-activating factor receptor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL353354.36, CD013879.1 and AL137792.11. On Aug 23, 2011 this sequence version replaced NM_001164723.1. Transcript Variant: This variant (4) lacks the 5' exon, but has an additional exon in the 5' region, as compared to variant 1. Variants 1-4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CD013879.1, DA672102.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149004, SAMN01820691 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGCCAC ATGACTCCTC CCACATGGAC TCTGAGTTCC GATACACTCT CTTCCCGATT 
GTTTACAGCA TCATCTTTGT GCTCGGGGTC ATTGCTAATG GCTACGTGCT GTGGGTCTTT 
GCCCGCCTGT ACCCTTGCAA GAAATTCAAT GAGATAAAGA TCTTCATGGT GAACCTCACC 
ATGGCGGACA TGCTCTTCTT GATCACCCTG CCACTTTGGA TTGTCTACTA CCAAAACCAG 
GGCAACTGGA TACTCCCCAA ATTCCTGTGC AACGTGGCTG GCTGCCTTTT CTTCATCAAC 
ACCTACTGCT CTGTGGCCTT CCTGGGCGTC ATCACTTATA ACCGCTTCCA GGCAGTAACT 
CGGCCCATCA AGACTGCTCA GGCCAACACC CGCAAGCGTG GCATCTCTTT GTCCTTGGTC 
ATCTGGGTGG CCATTGTGGG AGCTGCATCC TACTTCCTCA TCCTGGACTC CACCAACACA 
GTGCCCGACA GTGCTGGCTC AGGCAACGTC ACTCGCTGCT TTGAGCATTA CGAGAAGGGC 
AGCGTGCCAG TCCTCATCAT CCACATCTTC ATCGTGTTCA GCTTCTTCCT GGTCTTCCTC 
ATCATCCTCT TCTGCAACCT GGTCATCATC CGTACCTTGC TCATGCAGCC GGTGCAGCAG 
CAGCGCAACG CTGAAGTCAA GCGCCGGGCG CTGTGGATGG TGTGCACGGT CTTGGCGGTG 
TTCATCATCT GCTTCGTGCC CCACCACGTG GTGCAGCTGC CCTGGACCCT TGCTGAGCTG 
GGCTTCCAGG ACAGCAAATT CCACCAGGCC ATTAATGATG CACATCAGGT CACCCTCTGC 
CTCCTTAGCA CCAACTGTGT CTTAGACCCT GTTATCTACT GTTTCCTCAC CAAGAAGTTC 
CGCAAGCACC TCACCGAAAA GTTCTACAGC ATGCGCAGTA GCCGGAAATG CTCCCGGGCC 
ACCACGGATA CGGTCACTGA AGTGGTTGTG CCATTCAACC AGATCCCTGG CAATTCCCTC 
AAAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158195.1
CDS	463..1491
Translation	MEPHDSSHMDSEFRYTLFPIVYSIIFVLGVIANGYVLWVFARLYPCKKFNEIKIFMVNLTMADMLFLITLPLWIVYYQNQGNWILPKFLCNVAGCLFFINTYCSVAFLGVITYNRFQAVTRPIKTAQANTRKRGISLSLVIWVAIVGAASYFLILDSTNTVPDSAGSGNVTRCFEHYEKGSVPVLIIHIFIVFSFFLVFLIILFCNLVIIRTLLMQPVQQQRNAEVKRRALWMVCTVLAVFIICFVPHHVVQLPWTLAELGFQDSKFHQAINDAHQVTLCLLSTNCVLDPVIYCFLTKKFRKHLTEKFYSMRSSRKCSRATTDTVTEVVVPFNQIPGNSLKN

Target ORF information:

RefSeq Version	NM_001164723.2
Organism	Homo sapiens(human)
Definition	Homo sapiens platelet activating factor receptor (PTAFR), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164723.2

ORF Insert Sequence:

ATGGAGCCAC ATGACTCCTC CCACATGGAC TCTGAGTTCC GATACACTCT CTTCCCGATT 
GTTTACAGCA TCATCTTTGT GCTCGGGGTC ATTGCTAATG GCTACGTGCT GTGGGTCTTT 
GCCCGCCTGT ACCCTTGCAA GAAATTCAAT GAGATAAAGA TCTTCATGGT GAACCTCACC 
ATGGCGGACA TGCTCTTCTT GATCACCCTG CCACTTTGGA TTGTCTACTA CCAAAACCAG 
GGCAACTGGA TACTCCCCAA ATTCCTGTGC AACGTGGCTG GCTGCCTTTT CTTCATCAAC 
ACCTACTGCT CTGTGGCCTT CCTGGGCGTC ATCACTTATA ACCGCTTCCA GGCAGTAACT 
CGGCCCATCA AGACTGCTCA GGCCAACACC CGCAAGCGTG GCATCTCTTT GTCCTTGGTC 
ATCTGGGTGG CCATTGTGGG AGCTGCATCC TACTTCCTCA TCCTGGACTC CACCAACACA 
GTGCCCGACA GTGCTGGCTC AGGCAACGTC ACTCGCTGCT TTGAGCATTA CGAGAAGGGC 
AGCGTGCCAG TCCTCATCAT CCACATCTTC ATCGTGTTCA GCTTCTTCCT GGTCTTCCTC 
ATCATCCTCT TCTGCAACCT GGTCATCATC CGTACCTTGC TCATGCAGCC GGTGCAGCAG 
CAGCGCAACG CTGAAGTCAA GCGCCGGGCG CTGTGGATGG TGTGCACGGT CTTGGCGGTG 
TTCATCATCT GCTTCGTGCC CCACCACGTG GTGCAGCTGC CCTGGACCCT TGCTGAGCTG 
GGCTTCCAGG ACAGCAAATT CCACCAGGCC ATTAATGATG CACATCAGGT CACCCTCTGC 
CTCCTTAGCA CCAACTGTGT CTTAGACCCT GTTATCTACT GTTTCCTCAC CAAGAAGTTC 
CGCAAGCACC TCACCGAAAA GTTCTACAGC ATGCGCAGTA GCCGGAAATG CTCCCGGGCC 
ACCACGGATA CGGTCACTGA AGTGGTTGTG CCATTCAACC AGATCCCTGG CAATTCCCTC 
AAAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTAFR ( NM_001164723.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001158195.1 Homo sapiens platelet activating factor receptor (PTAFR), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu15818
Clone ID Related Accession (Same CDS sequence)	NM_001164723.2 , NM_001164723.3 , NM_001164722.2 , NM_001164722.3 , NM_001164721.1 , NM_000952.4 , NM_000952.5
Accession Version	NM_001164723.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	platelet-activating factor receptor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL353354.36, CD013879.1 and AL137792.11. On May 31, 2019 this sequence version replaced NM_001164723.2. Transcript Variant: This variant (4) lacks the 5' exon, but has an additional exon in the 5' region, as compared to variant 1. Variants 1-4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CD013879.1, DA150761.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149004, SAMN01820691 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGCCAC ATGACTCCTC CCACATGGAC TCTGAGTTCC GATACACTCT CTTCCCGATT 
GTTTACAGCA TCATCTTTGT GCTCGGGGTC ATTGCTAATG GCTACGTGCT GTGGGTCTTT 
GCCCGCCTGT ACCCTTGCAA GAAATTCAAT GAGATAAAGA TCTTCATGGT GAACCTCACC 
ATGGCGGACA TGCTCTTCTT GATCACCCTG CCACTTTGGA TTGTCTACTA CCAAAACCAG 
GGCAACTGGA TACTCCCCAA ATTCCTGTGC AACGTGGCTG GCTGCCTTTT CTTCATCAAC 
ACCTACTGCT CTGTGGCCTT CCTGGGCGTC ATCACTTATA ACCGCTTCCA GGCAGTAACT 
CGGCCCATCA AGACTGCTCA GGCCAACACC CGCAAGCGTG GCATCTCTTT GTCCTTGGTC 
ATCTGGGTGG CCATTGTGGG AGCTGCATCC TACTTCCTCA TCCTGGACTC CACCAACACA 
GTGCCCGACA GTGCTGGCTC AGGCAACGTC ACTCGCTGCT TTGAGCATTA CGAGAAGGGC 
AGCGTGCCAG TCCTCATCAT CCACATCTTC ATCGTGTTCA GCTTCTTCCT GGTCTTCCTC 
ATCATCCTCT TCTGCAACCT GGTCATCATC CGTACCTTGC TCATGCAGCC GGTGCAGCAG 
CAGCGCAACG CTGAAGTCAA GCGCCGGGCG CTGTGGATGG TGTGCACGGT CTTGGCGGTG 
TTCATCATCT GCTTCGTGCC CCACCACGTG GTGCAGCTGC CCTGGACCCT TGCTGAGCTG 
GGCTTCCAGG ACAGCAAATT CCACCAGGCC ATTAATGATG CACATCAGGT CACCCTCTGC 
CTCCTTAGCA CCAACTGTGT CTTAGACCCT GTTATCTACT GTTTCCTCAC CAAGAAGTTC 
CGCAAGCACC TCACCGAAAA GTTCTACAGC ATGCGCAGTA GCCGGAAATG CTCCCGGGCC 
ACCACGGATA CGGTCACTGA AGTGGTTGTG CCATTCAACC AGATCCCTGG CAATTCCCTC 
AAAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158195.1
CDS	209..1237
Translation	MEPHDSSHMDSEFRYTLFPIVYSIIFVLGVIANGYVLWVFARLYPCKKFNEIKIFMVNLTMADMLFLITLPLWIVYYQNQGNWILPKFLCNVAGCLFFINTYCSVAFLGVITYNRFQAVTRPIKTAQANTRKRGISLSLVIWVAIVGAASYFLILDSTNTVPDSAGSGNVTRCFEHYEKGSVPVLIIHIFIVFSFFLVFLIILFCNLVIIRTLLMQPVQQQRNAEVKRRALWMVCTVLAVFIICFVPHHVVQLPWTLAELGFQDSKFHQAINDAHQVTLCLLSTNCVLDPVIYCFLTKKFRKHLTEKFYSMRSSRKCSRATTDTVTEVVVPFNQIPGNSLKN

Target ORF information:

RefSeq Version	NM_001164723.3
Organism	Homo sapiens(human)
Definition	Homo sapiens platelet activating factor receptor (PTAFR), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164723.3

ORF Insert Sequence:

ATGGAGCCAC ATGACTCCTC CCACATGGAC TCTGAGTTCC GATACACTCT CTTCCCGATT 
GTTTACAGCA TCATCTTTGT GCTCGGGGTC ATTGCTAATG GCTACGTGCT GTGGGTCTTT 
GCCCGCCTGT ACCCTTGCAA GAAATTCAAT GAGATAAAGA TCTTCATGGT GAACCTCACC 
ATGGCGGACA TGCTCTTCTT GATCACCCTG CCACTTTGGA TTGTCTACTA CCAAAACCAG 
GGCAACTGGA TACTCCCCAA ATTCCTGTGC AACGTGGCTG GCTGCCTTTT CTTCATCAAC 
ACCTACTGCT CTGTGGCCTT CCTGGGCGTC ATCACTTATA ACCGCTTCCA GGCAGTAACT 
CGGCCCATCA AGACTGCTCA GGCCAACACC CGCAAGCGTG GCATCTCTTT GTCCTTGGTC 
ATCTGGGTGG CCATTGTGGG AGCTGCATCC TACTTCCTCA TCCTGGACTC CACCAACACA 
GTGCCCGACA GTGCTGGCTC AGGCAACGTC ACTCGCTGCT TTGAGCATTA CGAGAAGGGC 
AGCGTGCCAG TCCTCATCAT CCACATCTTC ATCGTGTTCA GCTTCTTCCT GGTCTTCCTC 
ATCATCCTCT TCTGCAACCT GGTCATCATC CGTACCTTGC TCATGCAGCC GGTGCAGCAG 
CAGCGCAACG CTGAAGTCAA GCGCCGGGCG CTGTGGATGG TGTGCACGGT CTTGGCGGTG 
TTCATCATCT GCTTCGTGCC CCACCACGTG GTGCAGCTGC CCTGGACCCT TGCTGAGCTG 
GGCTTCCAGG ACAGCAAATT CCACCAGGCC ATTAATGATG CACATCAGGT CACCCTCTGC 
CTCCTTAGCA CCAACTGTGT CTTAGACCCT GTTATCTACT GTTTCCTCAC CAAGAAGTTC 
CGCAAGCACC TCACCGAAAA GTTCTACAGC ATGCGCAGTA GCCGGAAATG CTCCCGGGCC 
ACCACGGATA CGGTCACTGA AGTGGTTGTG CCATTCAACC AGATCCCTGG CAATTCCCTC 
AAAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTAFR ( NM_001164722.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001158194.1 Homo sapiens platelet activating factor receptor (PTAFR), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu15818
Clone ID Related Accession (Same CDS sequence)	NM_001164723.2 , NM_001164723.3 , NM_001164722.2 , NM_001164722.3 , NM_001164721.1 , NM_000952.4 , NM_000952.5
Accession Version	NM_001164722.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	platelet-activating factor receptor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292053.1, DA705644.1 and AL137792.11. On Aug 23, 2011 this sequence version replaced NM_001164722.1. Transcript Variant: This variant (2) lacks an internal 5' UTR exon, as compared to variant 1. Variants 1-4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: S52624.1, BP253190.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGCCAC ATGACTCCTC CCACATGGAC TCTGAGTTCC GATACACTCT CTTCCCGATT 
GTTTACAGCA TCATCTTTGT GCTCGGGGTC ATTGCTAATG GCTACGTGCT GTGGGTCTTT 
GCCCGCCTGT ACCCTTGCAA GAAATTCAAT GAGATAAAGA TCTTCATGGT GAACCTCACC 
ATGGCGGACA TGCTCTTCTT GATCACCCTG CCACTTTGGA TTGTCTACTA CCAAAACCAG 
GGCAACTGGA TACTCCCCAA ATTCCTGTGC AACGTGGCTG GCTGCCTTTT CTTCATCAAC 
ACCTACTGCT CTGTGGCCTT CCTGGGCGTC ATCACTTATA ACCGCTTCCA GGCAGTAACT 
CGGCCCATCA AGACTGCTCA GGCCAACACC CGCAAGCGTG GCATCTCTTT GTCCTTGGTC 
ATCTGGGTGG CCATTGTGGG AGCTGCATCC TACTTCCTCA TCCTGGACTC CACCAACACA 
GTGCCCGACA GTGCTGGCTC AGGCAACGTC ACTCGCTGCT TTGAGCATTA CGAGAAGGGC 
AGCGTGCCAG TCCTCATCAT CCACATCTTC ATCGTGTTCA GCTTCTTCCT GGTCTTCCTC 
ATCATCCTCT TCTGCAACCT GGTCATCATC CGTACCTTGC TCATGCAGCC GGTGCAGCAG 
CAGCGCAACG CTGAAGTCAA GCGCCGGGCG CTGTGGATGG TGTGCACGGT CTTGGCGGTG 
TTCATCATCT GCTTCGTGCC CCACCACGTG GTGCAGCTGC CCTGGACCCT TGCTGAGCTG 
GGCTTCCAGG ACAGCAAATT CCACCAGGCC ATTAATGATG CACATCAGGT CACCCTCTGC 
CTCCTTAGCA CCAACTGTGT CTTAGACCCT GTTATCTACT GTTTCCTCAC CAAGAAGTTC 
CGCAAGCACC TCACCGAAAA GTTCTACAGC ATGCGCAGTA GCCGGAAATG CTCCCGGGCC 
ACCACGGATA CGGTCACTGA AGTGGTTGTG CCATTCAACC AGATCCCTGG CAATTCCCTC 
AAAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158194.1
CDS	254..1282
Translation	MEPHDSSHMDSEFRYTLFPIVYSIIFVLGVIANGYVLWVFARLYPCKKFNEIKIFMVNLTMADMLFLITLPLWIVYYQNQGNWILPKFLCNVAGCLFFINTYCSVAFLGVITYNRFQAVTRPIKTAQANTRKRGISLSLVIWVAIVGAASYFLILDSTNTVPDSAGSGNVTRCFEHYEKGSVPVLIIHIFIVFSFFLVFLIILFCNLVIIRTLLMQPVQQQRNAEVKRRALWMVCTVLAVFIICFVPHHVVQLPWTLAELGFQDSKFHQAINDAHQVTLCLLSTNCVLDPVIYCFLTKKFRKHLTEKFYSMRSSRKCSRATTDTVTEVVVPFNQIPGNSLKN

Target ORF information:

RefSeq Version	NM_001164722.2
Organism	Homo sapiens(human)
Definition	Homo sapiens platelet activating factor receptor (PTAFR), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164722.2

ORF Insert Sequence:

ATGGAGCCAC ATGACTCCTC CCACATGGAC TCTGAGTTCC GATACACTCT CTTCCCGATT 
GTTTACAGCA TCATCTTTGT GCTCGGGGTC ATTGCTAATG GCTACGTGCT GTGGGTCTTT 
GCCCGCCTGT ACCCTTGCAA GAAATTCAAT GAGATAAAGA TCTTCATGGT GAACCTCACC 
ATGGCGGACA TGCTCTTCTT GATCACCCTG CCACTTTGGA TTGTCTACTA CCAAAACCAG 
GGCAACTGGA TACTCCCCAA ATTCCTGTGC AACGTGGCTG GCTGCCTTTT CTTCATCAAC 
ACCTACTGCT CTGTGGCCTT CCTGGGCGTC ATCACTTATA ACCGCTTCCA GGCAGTAACT 
CGGCCCATCA AGACTGCTCA GGCCAACACC CGCAAGCGTG GCATCTCTTT GTCCTTGGTC 
ATCTGGGTGG CCATTGTGGG AGCTGCATCC TACTTCCTCA TCCTGGACTC CACCAACACA 
GTGCCCGACA GTGCTGGCTC AGGCAACGTC ACTCGCTGCT TTGAGCATTA CGAGAAGGGC 
AGCGTGCCAG TCCTCATCAT CCACATCTTC ATCGTGTTCA GCTTCTTCCT GGTCTTCCTC 
ATCATCCTCT TCTGCAACCT GGTCATCATC CGTACCTTGC TCATGCAGCC GGTGCAGCAG 
CAGCGCAACG CTGAAGTCAA GCGCCGGGCG CTGTGGATGG TGTGCACGGT CTTGGCGGTG 
TTCATCATCT GCTTCGTGCC CCACCACGTG GTGCAGCTGC CCTGGACCCT TGCTGAGCTG 
GGCTTCCAGG ACAGCAAATT CCACCAGGCC ATTAATGATG CACATCAGGT CACCCTCTGC 
CTCCTTAGCA CCAACTGTGT CTTAGACCCT GTTATCTACT GTTTCCTCAC CAAGAAGTTC 
CGCAAGCACC TCACCGAAAA GTTCTACAGC ATGCGCAGTA GCCGGAAATG CTCCCGGGCC 
ACCACGGATA CGGTCACTGA AGTGGTTGTG CCATTCAACC AGATCCCTGG CAATTCCCTC 
AAAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTAFR ( NM_001164722.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001158194.1 Homo sapiens platelet activating factor receptor (PTAFR), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu15818
Clone ID Related Accession (Same CDS sequence)	NM_001164723.2 , NM_001164723.3 , NM_001164722.2 , NM_001164722.3 , NM_001164721.1 , NM_000952.4 , NM_000952.5
Accession Version	NM_001164722.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-02
Organism	Homo sapiens(human)
Product	platelet-activating factor receptor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA705644.1 and AL137792.11. On May 31, 2019 this sequence version replaced NM_001164722.2. Transcript Variant: This variant (2) lacks an internal 5' UTR exon, as compared to variant 1. Variants 1-4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: S52624.1, BP253379.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGCCAC ATGACTCCTC CCACATGGAC TCTGAGTTCC GATACACTCT CTTCCCGATT 
GTTTACAGCA TCATCTTTGT GCTCGGGGTC ATTGCTAATG GCTACGTGCT GTGGGTCTTT 
GCCCGCCTGT ACCCTTGCAA GAAATTCAAT GAGATAAAGA TCTTCATGGT GAACCTCACC 
ATGGCGGACA TGCTCTTCTT GATCACCCTG CCACTTTGGA TTGTCTACTA CCAAAACCAG 
GGCAACTGGA TACTCCCCAA ATTCCTGTGC AACGTGGCTG GCTGCCTTTT CTTCATCAAC 
ACCTACTGCT CTGTGGCCTT CCTGGGCGTC ATCACTTATA ACCGCTTCCA GGCAGTAACT 
CGGCCCATCA AGACTGCTCA GGCCAACACC CGCAAGCGTG GCATCTCTTT GTCCTTGGTC 
ATCTGGGTGG CCATTGTGGG AGCTGCATCC TACTTCCTCA TCCTGGACTC CACCAACACA 
GTGCCCGACA GTGCTGGCTC AGGCAACGTC ACTCGCTGCT TTGAGCATTA CGAGAAGGGC 
AGCGTGCCAG TCCTCATCAT CCACATCTTC ATCGTGTTCA GCTTCTTCCT GGTCTTCCTC 
ATCATCCTCT TCTGCAACCT GGTCATCATC CGTACCTTGC TCATGCAGCC GGTGCAGCAG 
CAGCGCAACG CTGAAGTCAA GCGCCGGGCG CTGTGGATGG TGTGCACGGT CTTGGCGGTG 
TTCATCATCT GCTTCGTGCC CCACCACGTG GTGCAGCTGC CCTGGACCCT TGCTGAGCTG 
GGCTTCCAGG ACAGCAAATT CCACCAGGCC ATTAATGATG CACATCAGGT CACCCTCTGC 
CTCCTTAGCA CCAACTGTGT CTTAGACCCT GTTATCTACT GTTTCCTCAC CAAGAAGTTC 
CGCAAGCACC TCACCGAAAA GTTCTACAGC ATGCGCAGTA GCCGGAAATG CTCCCGGGCC 
ACCACGGATA CGGTCACTGA AGTGGTTGTG CCATTCAACC AGATCCCTGG CAATTCCCTC 
AAAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158194.1
CDS	174..1202
Translation	MEPHDSSHMDSEFRYTLFPIVYSIIFVLGVIANGYVLWVFARLYPCKKFNEIKIFMVNLTMADMLFLITLPLWIVYYQNQGNWILPKFLCNVAGCLFFINTYCSVAFLGVITYNRFQAVTRPIKTAQANTRKRGISLSLVIWVAIVGAASYFLILDSTNTVPDSAGSGNVTRCFEHYEKGSVPVLIIHIFIVFSFFLVFLIILFCNLVIIRTLLMQPVQQQRNAEVKRRALWMVCTVLAVFIICFVPHHVVQLPWTLAELGFQDSKFHQAINDAHQVTLCLLSTNCVLDPVIYCFLTKKFRKHLTEKFYSMRSSRKCSRATTDTVTEVVVPFNQIPGNSLKN

Target ORF information:

RefSeq Version	NM_001164722.3
Organism	Homo sapiens(human)
Definition	Homo sapiens platelet activating factor receptor (PTAFR), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164722.3

ORF Insert Sequence:

ATGGAGCCAC ATGACTCCTC CCACATGGAC TCTGAGTTCC GATACACTCT CTTCCCGATT 
GTTTACAGCA TCATCTTTGT GCTCGGGGTC ATTGCTAATG GCTACGTGCT GTGGGTCTTT 
GCCCGCCTGT ACCCTTGCAA GAAATTCAAT GAGATAAAGA TCTTCATGGT GAACCTCACC 
ATGGCGGACA TGCTCTTCTT GATCACCCTG CCACTTTGGA TTGTCTACTA CCAAAACCAG 
GGCAACTGGA TACTCCCCAA ATTCCTGTGC AACGTGGCTG GCTGCCTTTT CTTCATCAAC 
ACCTACTGCT CTGTGGCCTT CCTGGGCGTC ATCACTTATA ACCGCTTCCA GGCAGTAACT 
CGGCCCATCA AGACTGCTCA GGCCAACACC CGCAAGCGTG GCATCTCTTT GTCCTTGGTC 
ATCTGGGTGG CCATTGTGGG AGCTGCATCC TACTTCCTCA TCCTGGACTC CACCAACACA 
GTGCCCGACA GTGCTGGCTC AGGCAACGTC ACTCGCTGCT TTGAGCATTA CGAGAAGGGC 
AGCGTGCCAG TCCTCATCAT CCACATCTTC ATCGTGTTCA GCTTCTTCCT GGTCTTCCTC 
ATCATCCTCT TCTGCAACCT GGTCATCATC CGTACCTTGC TCATGCAGCC GGTGCAGCAG 
CAGCGCAACG CTGAAGTCAA GCGCCGGGCG CTGTGGATGG TGTGCACGGT CTTGGCGGTG 
TTCATCATCT GCTTCGTGCC CCACCACGTG GTGCAGCTGC CCTGGACCCT TGCTGAGCTG 
GGCTTCCAGG ACAGCAAATT CCACCAGGCC ATTAATGATG CACATCAGGT CACCCTCTGC 
CTCCTTAGCA CCAACTGTGT CTTAGACCCT GTTATCTACT GTTTCCTCAC CAAGAAGTTC 
CGCAAGCACC TCACCGAAAA GTTCTACAGC ATGCGCAGTA GCCGGAAATG CTCCCGGGCC 
ACCACGGATA CGGTCACTGA AGTGGTTGTG CCATTCAACC AGATCCCTGG CAATTCCCTC 
AAAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTAFR ( NM_001164721.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001158193.1 Homo sapiens platelet activating factor receptor (PTAFR), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu15818
Clone ID Related Accession (Same CDS sequence)	NM_001164723.2 , NM_001164723.3 , NM_001164722.2 , NM_001164722.3 , NM_001164721.1 , NM_000952.4 , NM_000952.5
Accession Version	NM_001164721.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-23
Organism	Homo sapiens(human)
Product	platelet-activating factor receptor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292053.1, DA705644.1, BC063000.1 and AL137792.11. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) is composed of three exons. Variants 1-4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292053.1, BP255271.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2142670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGCCAC ATGACTCCTC CCACATGGAC TCTGAGTTCC GATACACTCT CTTCCCGATT 
GTTTACAGCA TCATCTTTGT GCTCGGGGTC ATTGCTAATG GCTACGTGCT GTGGGTCTTT 
GCCCGCCTGT ACCCTTGCAA GAAATTCAAT GAGATAAAGA TCTTCATGGT GAACCTCACC 
ATGGCGGACA TGCTCTTCTT GATCACCCTG CCACTTTGGA TTGTCTACTA CCAAAACCAG 
GGCAACTGGA TACTCCCCAA ATTCCTGTGC AACGTGGCTG GCTGCCTTTT CTTCATCAAC 
ACCTACTGCT CTGTGGCCTT CCTGGGCGTC ATCACTTATA ACCGCTTCCA GGCAGTAACT 
CGGCCCATCA AGACTGCTCA GGCCAACACC CGCAAGCGTG GCATCTCTTT GTCCTTGGTC 
ATCTGGGTGG CCATTGTGGG AGCTGCATCC TACTTCCTCA TCCTGGACTC CACCAACACA 
GTGCCCGACA GTGCTGGCTC AGGCAACGTC ACTCGCTGCT TTGAGCATTA CGAGAAGGGC 
AGCGTGCCAG TCCTCATCAT CCACATCTTC ATCGTGTTCA GCTTCTTCCT GGTCTTCCTC 
ATCATCCTCT TCTGCAACCT GGTCATCATC CGTACCTTGC TCATGCAGCC GGTGCAGCAG 
CAGCGCAACG CTGAAGTCAA GCGCCGGGCG CTGTGGATGG TGTGCACGGT CTTGGCGGTG 
TTCATCATCT GCTTCGTGCC CCACCACGTG GTGCAGCTGC CCTGGACCCT TGCTGAGCTG 
GGCTTCCAGG ACAGCAAATT CCACCAGGCC ATTAATGATG CACATCAGGT CACCCTCTGC 
CTCCTTAGCA CCAACTGTGT CTTAGACCCT GTTATCTACT GTTTCCTCAC CAAGAAGTTC 
CGCAAGCACC TCACCGAAAA GTTCTACAGC ATGCGCAGTA GCCGGAAATG CTCCCGGGCC 
ACCACGGATA CGGTCACTGA AGTGGTTGTG CCATTCAACC AGATCCCTGG CAATTCCCTC 
AAAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158193.1
CDS	336..1364
Translation	MEPHDSSHMDSEFRYTLFPIVYSIIFVLGVIANGYVLWVFARLYPCKKFNEIKIFMVNLTMADMLFLITLPLWIVYYQNQGNWILPKFLCNVAGCLFFINTYCSVAFLGVITYNRFQAVTRPIKTAQANTRKRGISLSLVIWVAIVGAASYFLILDSTNTVPDSAGSGNVTRCFEHYEKGSVPVLIIHIFIVFSFFLVFLIILFCNLVIIRTLLMQPVQQQRNAEVKRRALWMVCTVLAVFIICFVPHHVVQLPWTLAELGFQDSKFHQAINDAHQVTLCLLSTNCVLDPVIYCFLTKKFRKHLTEKFYSMRSSRKCSRATTDTVTEVVVPFNQIPGNSLKN

Target ORF information:

RefSeq Version	NM_001164721.1
Organism	Homo sapiens(human)
Definition	Homo sapiens platelet activating factor receptor (PTAFR), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164721.1

ORF Insert Sequence:

ATGGAGCCAC ATGACTCCTC CCACATGGAC TCTGAGTTCC GATACACTCT CTTCCCGATT 
GTTTACAGCA TCATCTTTGT GCTCGGGGTC ATTGCTAATG GCTACGTGCT GTGGGTCTTT 
GCCCGCCTGT ACCCTTGCAA GAAATTCAAT GAGATAAAGA TCTTCATGGT GAACCTCACC 
ATGGCGGACA TGCTCTTCTT GATCACCCTG CCACTTTGGA TTGTCTACTA CCAAAACCAG 
GGCAACTGGA TACTCCCCAA ATTCCTGTGC AACGTGGCTG GCTGCCTTTT CTTCATCAAC 
ACCTACTGCT CTGTGGCCTT CCTGGGCGTC ATCACTTATA ACCGCTTCCA GGCAGTAACT 
CGGCCCATCA AGACTGCTCA GGCCAACACC CGCAAGCGTG GCATCTCTTT GTCCTTGGTC 
ATCTGGGTGG CCATTGTGGG AGCTGCATCC TACTTCCTCA TCCTGGACTC CACCAACACA 
GTGCCCGACA GTGCTGGCTC AGGCAACGTC ACTCGCTGCT TTGAGCATTA CGAGAAGGGC 
AGCGTGCCAG TCCTCATCAT CCACATCTTC ATCGTGTTCA GCTTCTTCCT GGTCTTCCTC 
ATCATCCTCT TCTGCAACCT GGTCATCATC CGTACCTTGC TCATGCAGCC GGTGCAGCAG 
CAGCGCAACG CTGAAGTCAA GCGCCGGGCG CTGTGGATGG TGTGCACGGT CTTGGCGGTG 
TTCATCATCT GCTTCGTGCC CCACCACGTG GTGCAGCTGC CCTGGACCCT TGCTGAGCTG 
GGCTTCCAGG ACAGCAAATT CCACCAGGCC ATTAATGATG CACATCAGGT CACCCTCTGC 
CTCCTTAGCA CCAACTGTGT CTTAGACCCT GTTATCTACT GTTTCCTCAC CAAGAAGTTC 
CGCAAGCACC TCACCGAAAA GTTCTACAGC ATGCGCAGTA GCCGGAAATG CTCCCGGGCC 
ACCACGGATA CGGTCACTGA AGTGGTTGTG CCATTCAACC AGATCCCTGG CAATTCCCTC 
AAAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTAFR ( NM_000952.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000943.1 Homo sapiens platelet activating factor receptor (PTAFR), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu15818
Clone ID Related Accession (Same CDS sequence)	NM_001164723.2 , NM_001164723.3 , NM_001164722.2 , NM_001164722.3 , NM_001164721.1 , NM_000952.4 , NM_000952.5
Accession Version	NM_000952.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-24
Organism	Homo sapiens(human)
Product	platelet-activating factor receptor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA923972.1, BC063000.1 and AL137792.11. On Sep 12, 2009 this sequence version replaced NM_000952.3. Transcript Variant: This variant (3) lacks the 5' exon, but has an alternate 5' segment, as compared to variant 1. Variants 1-4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DA923972.1, SRR1803613.40901.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGCCAC ATGACTCCTC CCACATGGAC TCTGAGTTCC GATACACTCT CTTCCCGATT 
GTTTACAGCA TCATCTTTGT GCTCGGGGTC ATTGCTAATG GCTACGTGCT GTGGGTCTTT 
GCCCGCCTGT ACCCTTGCAA GAAATTCAAT GAGATAAAGA TCTTCATGGT GAACCTCACC 
ATGGCGGACA TGCTCTTCTT GATCACCCTG CCACTTTGGA TTGTCTACTA CCAAAACCAG 
GGCAACTGGA TACTCCCCAA ATTCCTGTGC AACGTGGCTG GCTGCCTTTT CTTCATCAAC 
ACCTACTGCT CTGTGGCCTT CCTGGGCGTC ATCACTTATA ACCGCTTCCA GGCAGTAACT 
CGGCCCATCA AGACTGCTCA GGCCAACACC CGCAAGCGTG GCATCTCTTT GTCCTTGGTC 
ATCTGGGTGG CCATTGTGGG AGCTGCATCC TACTTCCTCA TCCTGGACTC CACCAACACA 
GTGCCCGACA GTGCTGGCTC AGGCAACGTC ACTCGCTGCT TTGAGCATTA CGAGAAGGGC 
AGCGTGCCAG TCCTCATCAT CCACATCTTC ATCGTGTTCA GCTTCTTCCT GGTCTTCCTC 
ATCATCCTCT TCTGCAACCT GGTCATCATC CGTACCTTGC TCATGCAGCC GGTGCAGCAG 
CAGCGCAACG CTGAAGTCAA GCGCCGGGCG CTGTGGATGG TGTGCACGGT CTTGGCGGTG 
TTCATCATCT GCTTCGTGCC CCACCACGTG GTGCAGCTGC CCTGGACCCT TGCTGAGCTG 
GGCTTCCAGG ACAGCAAATT CCACCAGGCC ATTAATGATG CACATCAGGT CACCCTCTGC 
CTCCTTAGCA CCAACTGTGT CTTAGACCCT GTTATCTACT GTTTCCTCAC CAAGAAGTTC 
CGCAAGCACC TCACCGAAAA GTTCTACAGC ATGCGCAGTA GCCGGAAATG CTCCCGGGCC 
ACCACGGATA CGGTCACTGA AGTGGTTGTG CCATTCAACC AGATCCCTGG CAATTCCCTC 
AAAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000943.1
CDS	392..1420
Translation	MEPHDSSHMDSEFRYTLFPIVYSIIFVLGVIANGYVLWVFARLYPCKKFNEIKIFMVNLTMADMLFLITLPLWIVYYQNQGNWILPKFLCNVAGCLFFINTYCSVAFLGVITYNRFQAVTRPIKTAQANTRKRGISLSLVIWVAIVGAASYFLILDSTNTVPDSAGSGNVTRCFEHYEKGSVPVLIIHIFIVFSFFLVFLIILFCNLVIIRTLLMQPVQQQRNAEVKRRALWMVCTVLAVFIICFVPHHVVQLPWTLAELGFQDSKFHQAINDAHQVTLCLLSTNCVLDPVIYCFLTKKFRKHLTEKFYSMRSSRKCSRATTDTVTEVVVPFNQIPGNSLKN

Target ORF information:

RefSeq Version	NM_000952.4
Organism	Homo sapiens(human)
Definition	Homo sapiens platelet activating factor receptor (PTAFR), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000952.4

ORF Insert Sequence:

ATGGAGCCAC ATGACTCCTC CCACATGGAC TCTGAGTTCC GATACACTCT CTTCCCGATT 
GTTTACAGCA TCATCTTTGT GCTCGGGGTC ATTGCTAATG GCTACGTGCT GTGGGTCTTT 
GCCCGCCTGT ACCCTTGCAA GAAATTCAAT GAGATAAAGA TCTTCATGGT GAACCTCACC 
ATGGCGGACA TGCTCTTCTT GATCACCCTG CCACTTTGGA TTGTCTACTA CCAAAACCAG 
GGCAACTGGA TACTCCCCAA ATTCCTGTGC AACGTGGCTG GCTGCCTTTT CTTCATCAAC 
ACCTACTGCT CTGTGGCCTT CCTGGGCGTC ATCACTTATA ACCGCTTCCA GGCAGTAACT 
CGGCCCATCA AGACTGCTCA GGCCAACACC CGCAAGCGTG GCATCTCTTT GTCCTTGGTC 
ATCTGGGTGG CCATTGTGGG AGCTGCATCC TACTTCCTCA TCCTGGACTC CACCAACACA 
GTGCCCGACA GTGCTGGCTC AGGCAACGTC ACTCGCTGCT TTGAGCATTA CGAGAAGGGC 
AGCGTGCCAG TCCTCATCAT CCACATCTTC ATCGTGTTCA GCTTCTTCCT GGTCTTCCTC 
ATCATCCTCT TCTGCAACCT GGTCATCATC CGTACCTTGC TCATGCAGCC GGTGCAGCAG 
CAGCGCAACG CTGAAGTCAA GCGCCGGGCG CTGTGGATGG TGTGCACGGT CTTGGCGGTG 
TTCATCATCT GCTTCGTGCC CCACCACGTG GTGCAGCTGC CCTGGACCCT TGCTGAGCTG 
GGCTTCCAGG ACAGCAAATT CCACCAGGCC ATTAATGATG CACATCAGGT CACCCTCTGC 
CTCCTTAGCA CCAACTGTGT CTTAGACCCT GTTATCTACT GTTTCCTCAC CAAGAAGTTC 
CGCAAGCACC TCACCGAAAA GTTCTACAGC ATGCGCAGTA GCCGGAAATG CTCCCGGGCC 
ACCACGGATA CGGTCACTGA AGTGGTTGTG CCATTCAACC AGATCCCTGG CAATTCCCTC 
AAAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PTAFR ( NM_000952.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000943.1 Homo sapiens platelet activating factor receptor (PTAFR), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu15818
Clone ID Related Accession (Same CDS sequence)	NM_001164723.2 , NM_001164723.3 , NM_001164722.2 , NM_001164722.3 , NM_001164721.1 , NM_000952.4 , NM_000952.5
Accession Version	NM_000952.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	platelet-activating factor receptor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA923972.1, BC063000.1 and AL137792.11. On Nov 22, 2018 this sequence version replaced NM_000952.4. Transcript Variant: This variant (3) lacks the 5' exon, but has an alternate 5' segment, as compared to variant 1. Variants 1-4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK290240.1, SRR3476690.162927.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000373857.8/ ENSP00000362965.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGCCAC ATGACTCCTC CCACATGGAC TCTGAGTTCC GATACACTCT CTTCCCGATT 
GTTTACAGCA TCATCTTTGT GCTCGGGGTC ATTGCTAATG GCTACGTGCT GTGGGTCTTT 
GCCCGCCTGT ACCCTTGCAA GAAATTCAAT GAGATAAAGA TCTTCATGGT GAACCTCACC 
ATGGCGGACA TGCTCTTCTT GATCACCCTG CCACTTTGGA TTGTCTACTA CCAAAACCAG 
GGCAACTGGA TACTCCCCAA ATTCCTGTGC AACGTGGCTG GCTGCCTTTT CTTCATCAAC 
ACCTACTGCT CTGTGGCCTT CCTGGGCGTC ATCACTTATA ACCGCTTCCA GGCAGTAACT 
CGGCCCATCA AGACTGCTCA GGCCAACACC CGCAAGCGTG GCATCTCTTT GTCCTTGGTC 
ATCTGGGTGG CCATTGTGGG AGCTGCATCC TACTTCCTCA TCCTGGACTC CACCAACACA 
GTGCCCGACA GTGCTGGCTC AGGCAACGTC ACTCGCTGCT TTGAGCATTA CGAGAAGGGC 
AGCGTGCCAG TCCTCATCAT CCACATCTTC ATCGTGTTCA GCTTCTTCCT GGTCTTCCTC 
ATCATCCTCT TCTGCAACCT GGTCATCATC CGTACCTTGC TCATGCAGCC GGTGCAGCAG 
CAGCGCAACG CTGAAGTCAA GCGCCGGGCG CTGTGGATGG TGTGCACGGT CTTGGCGGTG 
TTCATCATCT GCTTCGTGCC CCACCACGTG GTGCAGCTGC CCTGGACCCT TGCTGAGCTG 
GGCTTCCAGG ACAGCAAATT CCACCAGGCC ATTAATGATG CACATCAGGT CACCCTCTGC 
CTCCTTAGCA CCAACTGTGT CTTAGACCCT GTTATCTACT GTTTCCTCAC CAAGAAGTTC 
CGCAAGCACC TCACCGAAAA GTTCTACAGC ATGCGCAGTA GCCGGAAATG CTCCCGGGCC 
ACCACGGATA CGGTCACTGA AGTGGTTGTG CCATTCAACC AGATCCCTGG CAATTCCCTC 
AAAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000943.1
CDS	138..1166
Translation	MEPHDSSHMDSEFRYTLFPIVYSIIFVLGVIANGYVLWVFARLYPCKKFNEIKIFMVNLTMADMLFLITLPLWIVYYQNQGNWILPKFLCNVAGCLFFINTYCSVAFLGVITYNRFQAVTRPIKTAQANTRKRGISLSLVIWVAIVGAASYFLILDSTNTVPDSAGSGNVTRCFEHYEKGSVPVLIIHIFIVFSFFLVFLIILFCNLVIIRTLLMQPVQQQRNAEVKRRALWMVCTVLAVFIICFVPHHVVQLPWTLAELGFQDSKFHQAINDAHQVTLCLLSTNCVLDPVIYCFLTKKFRKHLTEKFYSMRSSRKCSRATTDTVTEVVVPFNQIPGNSLKN

Target ORF information:

RefSeq Version	NM_000952.5
Organism	Homo sapiens(human)
Definition	Homo sapiens platelet activating factor receptor (PTAFR), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000952.5

ORF Insert Sequence:

ATGGAGCCAC ATGACTCCTC CCACATGGAC TCTGAGTTCC GATACACTCT CTTCCCGATT 
GTTTACAGCA TCATCTTTGT GCTCGGGGTC ATTGCTAATG GCTACGTGCT GTGGGTCTTT 
GCCCGCCTGT ACCCTTGCAA GAAATTCAAT GAGATAAAGA TCTTCATGGT GAACCTCACC 
ATGGCGGACA TGCTCTTCTT GATCACCCTG CCACTTTGGA TTGTCTACTA CCAAAACCAG 
GGCAACTGGA TACTCCCCAA ATTCCTGTGC AACGTGGCTG GCTGCCTTTT CTTCATCAAC 
ACCTACTGCT CTGTGGCCTT CCTGGGCGTC ATCACTTATA ACCGCTTCCA GGCAGTAACT 
CGGCCCATCA AGACTGCTCA GGCCAACACC CGCAAGCGTG GCATCTCTTT GTCCTTGGTC 
ATCTGGGTGG CCATTGTGGG AGCTGCATCC TACTTCCTCA TCCTGGACTC CACCAACACA 
GTGCCCGACA GTGCTGGCTC AGGCAACGTC ACTCGCTGCT TTGAGCATTA CGAGAAGGGC 
AGCGTGCCAG TCCTCATCAT CCACATCTTC ATCGTGTTCA GCTTCTTCCT GGTCTTCCTC 
ATCATCCTCT TCTGCAACCT GGTCATCATC CGTACCTTGC TCATGCAGCC GGTGCAGCAG 
CAGCGCAACG CTGAAGTCAA GCGCCGGGCG CTGTGGATGG TGTGCACGGT CTTGGCGGTG 
TTCATCATCT GCTTCGTGCC CCACCACGTG GTGCAGCTGC CCTGGACCCT TGCTGAGCTG 
GGCTTCCAGG ACAGCAAATT CCACCAGGCC ATTAATGATG CACATCAGGT CACCCTCTGC 
CTCCTTAGCA CCAACTGTGT CTTAGACCCT GTTATCTACT GTTTCCTCAC CAAGAAGTTC 
CGCAAGCACC TCACCGAAAA GTTCTACAGC ATGCGCAGTA GCCGGAAATG CTCCCGGGCC 
ACCACGGATA CGGTCACTGA AGTGGTTGTG CCATTCAACC AGATCCCTGG CAATTCCCTC 
AAAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Association of platelet-activating factor receptor gene rs5938 (G/T) and rs313152 (T/C) polymorphisms with coronary heart disease and blood stasis syndrome in a Chinese Han population.
Chinese journal of integrative medicine23(12)893-900(2017 Dec)
Zheng GH,Xiong SQ,Chen HY,Mei LJ,Wang T

Weighted gene co-expression network analysis of gene modules for the prognosis of esophageal cancer.
Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban37(3)319-325(2017 Jun)
Zhang C,Sun Q

Systematic protein-protein interaction mapping for clinically relevant human GPCRs.
Molecular systems biology13(3)918(2017 03)
Sokolina K,Kittanakom S,Snider J,Kotlyar M,Maurice P,Gand?a J,Benleulmi-Chaachoua A,Tadagaki K,Oishi A,Wong V,Malty RH,Deineko V,Aoki H,Amin S,Yao Z,Morat? X,Otasek D,Kobayashi H,Menendez J,Auerbach D,Angers S,Pr?ulj N,Bouvier M,Babu M,Ciruela F,Jockers R,Jurisica I,Stagljar I

Pneumococcal infection of respiratory cells exposed to welding fumes; Role of oxidative stress and HIF-1 alpha.
PloS one12(3)e0173569(2017)
Grigg J,Miyashita L,Suri R

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PTAFR cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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