Phka1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Phka1
Entrez Gene ID	18679
Full Name	phosphorylase kinase alpha 1
Synonyms	5330411D17,9830108K24Rik,Phka
General protein information	Preferred Names phosphorylase kinase alpha 1 Names phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform phosphorylase kinase alpha M subunit
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X D\|X 45.47 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006527895.3	XP_006527958.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2
XM_017318427.1	XP_017173916.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X3
XM_006527894.3	XP_006527957.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
XM_006527894.4	XP_006527957.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
XM_017318427.2	XP_017173916.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X3
NM_008832.2	NP_032858.2	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform 1
XM_006527895.4	XP_006527958.1	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2
NM_173021.3	NP_766609.2	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform 2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	phka1	XP_005166575.1
Pan troglodytes (chimpanzee)	PHKA1	XP_003317571.2
Mus musculus (house mouse)	Phka1	NP_032858.2
Gallus gallus (chicken)	PHKA1	XP_004940655.1
	PHKA1	XP_001094193.1
Xenopus tropicalis (tropical clawed frog)	phka1	NP_001121510.1
Homo sapiens (human)	PHKA1	NP_002628.2
Canis lupus familiaris (dog)	PHKA1	XP_005641551.1
Bos taurus (cattle)	PHKA1	XP_005228049.1
Rattus norvegicus (Norway rat)	Phka1	NP_072148.1
Danio rerio (zebrafish)	si:dkey-22f5.10	XP_690880.4

Gene Ontology
Bibliography

Related articles in PubMed

Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series.
Bowman CE, Zhao L, Hartung T, Wolfgang MJ
Molecular and cellular biology36(15)2089-104(2016 Aug)

A postnatal switch of CELF and MBNL proteins reprograms alternative splicing in the developing heart.
Kalsotra A, Xiao X, Ward AJ, Castle JC, Johnson JM, Burge CB, Cooper TA
Proceedings of the National Academy of Sciences of the United States of America105(51)20333-8(2008 Dec)

Cloning and localization of the murine Xpct gene: evidence for complex rearrangements during the evolution of the region around the Xist gene.
Debrand E, Heard E, Avner P
Genomics48(3)296-303(1998 Mar)

Phosphorylase kinase deficiency in mice.
Huijing F
FEBS letters10(5)328-332(1970 Oct)

The relation of phosphorylase to glycogenolysis in skeletal muscle and heart of mice.
LYON JB Jr, PORTER J
The Journal of biological chemistry2381-11(1963 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Phka1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Phka1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu83092	XM_017318427.2 Latest version!	Mus musculus phosphorylase kinase alpha 1 (Phka1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu56199	XM_006527894.4 Latest version!	Mus musculus phosphorylase kinase alpha 1 (Phka1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OMu56200	XM_006527895.4 Latest version!	Mus musculus phosphorylase kinase alpha 1 (Phka1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu23486	NM_008832.2 Latest version!	Mus musculus phosphorylase kinase alpha 1 (Phka1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$727.30 $1039.00
OMu23500	NM_173021.3 Latest version!	Mus musculus phosphorylase kinase alpha 1 (Phka1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$692.30 $989.00
View Old Accession Versions >>
OMu56199	XM_006527894.3	Mus musculus phosphorylase kinase alpha 1 (Phka1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu83092	XM_017318427.1	Mus musculus phosphorylase kinase alpha 1 (Phka1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu56200	XM_006527895.3	Mus musculus phosphorylase kinase alpha 1 (Phka1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Phka1 ( XM_017318427.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173916.1 Mus musculus phosphorylase kinase alpha 1 (Phka1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu83092
Clone ID Related Accession (Same CDS sequence)	XM_017318427.1 , XM_017318427.2
Accession Version	XM_017318427.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCC GCAGTAACTC GGGAGTCCGC CTGGACGGCT ATGCTCGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACA GGCTTGCTTC CAGCCAGCTA TGATCAAAAA 
GATGCCTGGG TCCGAGATAA TGTGTACAGT ATCCTGGCTG TGTGGGGTTT GGGCCTGGCA 
TATCGCAAAA ATGCTGACCG TGATGAGGAC AAGGCAAAGG CCTATGAACT GGAGCAGAGT 
GTAGTGAAGT TAATGAGGGG ACTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAG 
TCCTTCAAGT ACAGTCAGAG TACTAAAGAT AGCCTCCATG CCAAGTACAA CACCAAAACT 
TGTGCCACTG TGGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACTTCTGTG 
TACCTGCTCT TCCTAGCACA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAC 
GAAGTCAATT TTATACAGAA CCTCGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTTGGGATAT GGGAACGTGG CGATAAGACA AACCAAGGCA TCTCGGAATT GAATGCGAGT 
TCAGTTGGAA TGGCCAAGGC AGCCCTGGAA GCACTAGATG AATTAGACTT GTTTGGTGTG 
AAAGGTGGGC CACAATCAGT TATCCATGTC CTGGCTGATG AAGTCCAACA CTGCCAGTCT 
ATCCTGAATT CACTACTGCC CAGGGCTTCA ACATCCAAAG AAGTTGATGC CAGTCTGCTC 
TCAGTGGTCT CTTTCCCAGC CTTTGCTGTA GAGGACAGCC ATTTGGTGGA GCTCACCAAA 
CAGGAGATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGTTTTCT GCGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGC CTATACTATG AACCAGCTGA GCTGAAGCTA 
TTTGAAAACA TTGAGTGCGA ATGGCCATTG TTCTGGACAT ACTTTATCCT TGATGGGATC 
TTCAGTGGCA ACGTAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGATGC AGTCCTCATC 
AAGGGCAAAA ATGGAGTCCC TCTTCTTCCA GAGCTGTACA GTGTCCCTCC TGACAGGGTT 
GATGAAGAGT ATCAAAATCC CCACACTGTG GATCGAGTCC CTATGGGAAA ATTGCCTCAC 
ATGTGGGGTC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTGAA TCGTAGGTTT TCTACTGTGC CAAAGCCAGA TGTGGTGGTT 
CAAGTCTCCA TTCTGGCTGA AACAGAAGAA ATCAAGGCCA TTTTGAAGGA CAAAGGAATT 
GATGTGGAGA CCATTGCTGA AGTGTACCCC ATAAGAGTAC AGCCAGCTCG TATTCTCAGC 
CATATTTATT CTAGTCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG 
CTCATGGGTG TGCTTGGAAC ATCAAAACTT TATGACATTC GCAAAACTAT CTTTACTTTC 
ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACCA GATGATAGTA 
GAAATGCTCA GAACAGACCT TTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC 
ACGATCACTT TCCCTATCTC GCACACCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCTTGG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGGGC CAGGATCCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGCTGCA CACACTTAAG CTTCATGGAC 
CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTATGATG AAGACTATGA AGATGATTTG 
GACTCTGGCA ACTGGATGGA CAGCTATGAT TCAACAAGTA ATGCTCGCTG TGGTGATGAA 
GTTGCCCGTT ATTTAGACCG CCTTTTGGCA CACACTGTTC CCCATCCTAA ACTAGCTCCT 
ACCTCACGGA AGGGAGGGCT AGATCGGTTC CGAGCTGCTG TGCAAACAAC TTGCGACTTA 
ATGTCCTTGG TGGCCAAGGC CAAGGAGCTG CATATACAGA ATGTTCACAT GTATCTTCCT 
ACAAAGTTAT TTCAGCCTTC TCGTCCTTCA CTCAACTTAC TTGACTCCCC TGAGTCTCCA 
CAAGATAGCC AGGTTCCTTC CGTTCATGTA GAAGTGCATC TTCCTAGGGA CCAGTCTGGG 
GAAGTGGACT TCCAGTCATT GGTTTCACAG TTGAAGGAGA CCTCAAGCTT ACAGGAGCAA 
GCTGATATAC TCTACATGCT GTATTCAATG AAGGTGATAC AGGAAAAAGC CTCATGGGGA 
GCCACCCTCT TTCAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173916.1
CDS	363..2783
Translation	MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSLLPRASTSKEVDASLLSVVSFPAFAVEDSHLVELTKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGIFSGNVEQVQEYREALDAVLIKGKNGVPLLPELYSVPPDRVDEEYQNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKAILKDKGIDVETIAEVYPIRVQPARILSHIYSSLGCNSRMKLSGRPYRLMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNQMIVEMLRTDLSYLCSRWRMTGQPTITFPISHTMLDEDGTSLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDEDYEDDLDSGNWMDSYDSTSNARCGDEVARYLDRLLAHTVPHPKLAPTSRKGGLDRFRAAVQTTCDLMSLVAKAKELHIQNVHMYLPTKLFQPSRPSLNLLDSPESPQDSQVPSVHVEVHLPRDQSGEVDFQSLVSQLKETSSLQEQADILYMLYSMKVIQEKASWGATLFQQ

Target ORF information:

RefSeq Version	XM_017318427.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphorylase kinase alpha 1 (Phka1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318427.1

ORF Insert Sequence:

ATGAGGAGCC GCAGTAACTC GGGAGTCCGC CTGGACGGCT ATGCTCGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACA GGCTTGCTTC CAGCCAGCTA TGATCAAAAA 
GATGCCTGGG TCCGAGATAA TGTGTACAGT ATCCTGGCTG TGTGGGGTTT GGGCCTGGCA 
TATCGCAAAA ATGCTGACCG TGATGAGGAC AAGGCAAAGG CCTATGAACT GGAGCAGAGT 
GTAGTGAAGT TAATGAGGGG ACTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAG 
TCCTTCAAGT ACAGTCAGAG TACTAAAGAT AGCCTCCATG CCAAGTACAA CACCAAAACT 
TGTGCCACTG TGGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACTTCTGTG 
TACCTGCTCT TCCTAGCACA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAC 
GAAGTCAATT TTATACAGAA CCTCGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTTGGGATAT GGGAACGTGG CGATAAGACA AACCAAGGCA TCTCGGAATT GAATGCGAGT 
TCAGTTGGAA TGGCCAAGGC AGCCCTGGAA GCACTAGATG AATTAGACTT GTTTGGTGTG 
AAAGGTGGGC CACAATCAGT TATCCATGTC CTGGCTGATG AAGTCCAACA CTGCCAGTCT 
ATCCTGAATT CACTACTGCC CAGGGCTTCA ACATCCAAAG AAGTTGATGC CAGTCTGCTC 
TCAGTGGTCT CTTTCCCAGC CTTTGCTGTA GAGGACAGCC ATTTGGTGGA GCTCACCAAA 
CAGGAGATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGTTTTCT GCGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGC CTATACTATG AACCAGCTGA GCTGAAGCTA 
TTTGAAAACA TTGAGTGCGA ATGGCCATTG TTCTGGACAT ACTTTATCCT TGATGGGATC 
TTCAGTGGCA ACGTAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGATGC AGTCCTCATC 
AAGGGCAAAA ATGGAGTCCC TCTTCTTCCA GAGCTGTACA GTGTCCCTCC TGACAGGGTT 
GATGAAGAGT ATCAAAATCC CCACACTGTG GATCGAGTCC CTATGGGAAA ATTGCCTCAC 
ATGTGGGGTC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTGAA TCGTAGGTTT TCTACTGTGC CAAAGCCAGA TGTGGTGGTT 
CAAGTCTCCA TTCTGGCTGA AACAGAAGAA ATCAAGGCCA TTTTGAAGGA CAAAGGAATT 
GATGTGGAGA CCATTGCTGA AGTGTACCCC ATAAGAGTAC AGCCAGCTCG TATTCTCAGC 
CATATTTATT CTAGTCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG 
CTCATGGGTG TGCTTGGAAC ATCAAAACTT TATGACATTC GCAAAACTAT CTTTACTTTC 
ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACCA GATGATAGTA 
GAAATGCTCA GAACAGACCT TTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC 
ACGATCACTT TCCCTATCTC GCACACCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCTTGG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGGGC CAGGATCCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGCTGCA CACACTTAAG CTTCATGGAC 
CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTATGATG AAGACTATGA AGATGATTTG 
GACTCTGGCA ACTGGATGGA CAGCTATGAT TCAACAAGTA ATGCTCGCTG TGGTGATGAA 
GTTGCCCGTT ATTTAGACCG CCTTTTGGCA CACACTGTTC CCCATCCTAA ACTAGCTCCT 
ACCTCACGGA AGGGAGGGCT AGATCGGTTC CGAGCTGCTG TGCAAACAAC TTGCGACTTA 
ATGTCCTTGG TGGCCAAGGC CAAGGAGCTG CATATACAGA ATGTTCACAT GTATCTTCCT 
ACAAAGTTAT TTCAGCCTTC TCGTCCTTCA CTCAACTTAC TTGACTCCCC TGAGTCTCCA 
CAAGATAGCC AGGTTCCTTC CGTTCATGTA GAAGTGCATC TTCCTAGGGA CCAGTCTGGG 
GAAGTGGACT TCCAGTCATT GGTTTCACAG TTGAAGGAGA CCTCAAGCTT ACAGGAGCAA 
GCTGATATAC TCTACATGCT GTATTCAATG AAGGTGATAC AGGAAAAAGC CTCATGGGGA 
GCCACCCTCT TTCAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phka1 ( XM_017318427.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017173916.1 Mus musculus phosphorylase kinase alpha 1 (Phka1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu83092
Clone ID Related Accession (Same CDS sequence)	XM_017318427.1 , XM_017318427.2
Accession Version	XM_017318427.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017318427.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCC GCAGTAACTC GGGAGTCCGC CTGGACGGCT ATGCTCGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACA GGCTTGCTTC CAGCCAGCTA TGATCAAAAA 
GATGCCTGGG TCCGAGATAA TGTGTACAGT ATCCTGGCTG TGTGGGGTTT GGGCCTGGCA 
TATCGCAAAA ATGCTGACCG TGATGAGGAC AAGGCAAAGG CCTATGAACT GGAGCAGAGT 
GTAGTGAAGT TAATGAGGGG ACTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAG 
TCCTTCAAGT ACAGTCAGAG TACTAAAGAT AGCCTCCATG CCAAGTACAA CACCAAAACT 
TGTGCCACTG TGGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACTTCTGTG 
TACCTGCTCT TCCTAGCACA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAC 
GAAGTCAATT TTATACAGAA CCTCGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTTGGGATAT GGGAACGTGG CGATAAGACA AACCAAGGCA TCTCGGAATT GAATGCGAGT 
TCAGTTGGAA TGGCCAAGGC AGCCCTGGAA GCACTAGATG AATTAGACTT GTTTGGTGTG 
AAAGGTGGGC CACAATCAGT TATCCATGTC CTGGCTGATG AAGTCCAACA CTGCCAGTCT 
ATCCTGAATT CACTACTGCC CAGGGCTTCA ACATCCAAAG AAGTTGATGC CAGTCTGCTC 
TCAGTGGTCT CTTTCCCAGC CTTTGCTGTA GAGGACAGCC ATTTGGTGGA GCTCACCAAA 
CAGGAGATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGTTTTCT GCGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGC CTATACTATG AACCAGCTGA GCTGAAGCTA 
TTTGAAAACA TTGAGTGCGA ATGGCCATTG TTCTGGACAT ACTTTATCCT TGATGGGATC 
TTCAGTGGCA ACGTAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGATGC AGTCCTCATC 
AAGGGCAAAA ATGGAGTCCC TCTTCTTCCA GAGCTGTACA GTGTCCCTCC TGACAGGGTT 
GATGAAGAGT ATCAAAATCC CCACACTGTG GATCGAGTCC CTATGGGAAA ATTGCCTCAC 
ATGTGGGGTC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTGAA TCGTAGGTTT TCTACTGTGC CAAAGCCAGA TGTGGTGGTT 
CAAGTCTCCA TTCTGGCTGA AACAGAAGAA ATCAAGGCCA TTTTGAAGGA CAAAGGAATT 
GATGTGGAGA CCATTGCTGA AGTGTACCCC ATAAGAGTAC AGCCAGCTCG TATTCTCAGC 
CATATTTATT CTAGTCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG 
CTCATGGGTG TGCTTGGAAC ATCAAAACTT TATGACATTC GCAAAACTAT CTTTACTTTC 
ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACCA GATGATAGTA 
GAAATGCTCA GAACAGACCT TTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC 
ACGATCACTT TCCCTATCTC GCACACCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCTTGG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGGGC CAGGATCCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGCTGCA CACACTTAAG CTTCATGGAC 
CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTATGATG AAGACTATGA AGATGATTTG 
GACTCTGGCA ACTGGATGGA CAGCTATGAT TCAACAAGTA ATGCTCGCTG TGGTGATGAA 
GTTGCCCGTT ATTTAGACCG CCTTTTGGCA CACACTGTTC CCCATCCTAA ACTAGCTCCT 
ACCTCACGGA AGGGAGGGCT AGATCGGTTC CGAGCTGCTG TGCAAACAAC TTGCGACTTA 
ATGTCCTTGG TGGCCAAGGC CAAGGAGCTG CATATACAGA ATGTTCACAT GTATCTTCCT 
ACAAAGTTAT TTCAGCCTTC TCGTCCTTCA CTCAACTTAC TTGACTCCCC TGAGTCTCCA 
CAAGATAGCC AGGTTCCTTC CGTTCATGTA GAAGTGCATC TTCCTAGGGA CCAGTCTGGG 
GAAGTGGACT TCCAGTCATT GGTTTCACAG TTGAAGGAGA CCTCAAGCTT ACAGGAGCAA 
GCTGATATAC TCTACATGCT GTATTCAATG AAGGTGATAC AGGAAAAAGC CTCATGGGGA 
GCCACCCTCT TTCAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017173916.1
CDS	378..2798
Translation	MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSLLPRASTSKEVDASLLSVVSFPAFAVEDSHLVELTKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGIFSGNVEQVQEYREALDAVLIKGKNGVPLLPELYSVPPDRVDEEYQNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKAILKDKGIDVETIAEVYPIRVQPARILSHIYSSLGCNSRMKLSGRPYRLMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNQMIVEMLRTDLSYLCSRWRMTGQPTITFPISHTMLDEDGTSLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDEDYEDDLDSGNWMDSYDSTSNARCGDEVARYLDRLLAHTVPHPKLAPTSRKGGLDRFRAAVQTTCDLMSLVAKAKELHIQNVHMYLPTKLFQPSRPSLNLLDSPESPQDSQVPSVHVEVHLPRDQSGEVDFQSLVSQLKETSSLQEQADILYMLYSMKVIQEKASWGATLFQQ

Target ORF information:

RefSeq Version	XM_017318427.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphorylase kinase alpha 1 (Phka1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017318427.2

ORF Insert Sequence:

ATGAGGAGCC GCAGTAACTC GGGAGTCCGC CTGGACGGCT ATGCTCGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACA GGCTTGCTTC CAGCCAGCTA TGATCAAAAA 
GATGCCTGGG TCCGAGATAA TGTGTACAGT ATCCTGGCTG TGTGGGGTTT GGGCCTGGCA 
TATCGCAAAA ATGCTGACCG TGATGAGGAC AAGGCAAAGG CCTATGAACT GGAGCAGAGT 
GTAGTGAAGT TAATGAGGGG ACTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAG 
TCCTTCAAGT ACAGTCAGAG TACTAAAGAT AGCCTCCATG CCAAGTACAA CACCAAAACT 
TGTGCCACTG TGGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACTTCTGTG 
TACCTGCTCT TCCTAGCACA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAC 
GAAGTCAATT TTATACAGAA CCTCGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTTGGGATAT GGGAACGTGG CGATAAGACA AACCAAGGCA TCTCGGAATT GAATGCGAGT 
TCAGTTGGAA TGGCCAAGGC AGCCCTGGAA GCACTAGATG AATTAGACTT GTTTGGTGTG 
AAAGGTGGGC CACAATCAGT TATCCATGTC CTGGCTGATG AAGTCCAACA CTGCCAGTCT 
ATCCTGAATT CACTACTGCC CAGGGCTTCA ACATCCAAAG AAGTTGATGC CAGTCTGCTC 
TCAGTGGTCT CTTTCCCAGC CTTTGCTGTA GAGGACAGCC ATTTGGTGGA GCTCACCAAA 
CAGGAGATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGTTTTCT GCGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGC CTATACTATG AACCAGCTGA GCTGAAGCTA 
TTTGAAAACA TTGAGTGCGA ATGGCCATTG TTCTGGACAT ACTTTATCCT TGATGGGATC 
TTCAGTGGCA ACGTAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGATGC AGTCCTCATC 
AAGGGCAAAA ATGGAGTCCC TCTTCTTCCA GAGCTGTACA GTGTCCCTCC TGACAGGGTT 
GATGAAGAGT ATCAAAATCC CCACACTGTG GATCGAGTCC CTATGGGAAA ATTGCCTCAC 
ATGTGGGGTC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTGAA TCGTAGGTTT TCTACTGTGC CAAAGCCAGA TGTGGTGGTT 
CAAGTCTCCA TTCTGGCTGA AACAGAAGAA ATCAAGGCCA TTTTGAAGGA CAAAGGAATT 
GATGTGGAGA CCATTGCTGA AGTGTACCCC ATAAGAGTAC AGCCAGCTCG TATTCTCAGC 
CATATTTATT CTAGTCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG 
CTCATGGGTG TGCTTGGAAC ATCAAAACTT TATGACATTC GCAAAACTAT CTTTACTTTC 
ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACCA GATGATAGTA 
GAAATGCTCA GAACAGACCT TTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC 
ACGATCACTT TCCCTATCTC GCACACCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCTTGG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGGGC CAGGATCCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGCTGCA CACACTTAAG CTTCATGGAC 
CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTATGATG AAGACTATGA AGATGATTTG 
GACTCTGGCA ACTGGATGGA CAGCTATGAT TCAACAAGTA ATGCTCGCTG TGGTGATGAA 
GTTGCCCGTT ATTTAGACCG CCTTTTGGCA CACACTGTTC CCCATCCTAA ACTAGCTCCT 
ACCTCACGGA AGGGAGGGCT AGATCGGTTC CGAGCTGCTG TGCAAACAAC TTGCGACTTA 
ATGTCCTTGG TGGCCAAGGC CAAGGAGCTG CATATACAGA ATGTTCACAT GTATCTTCCT 
ACAAAGTTAT TTCAGCCTTC TCGTCCTTCA CTCAACTTAC TTGACTCCCC TGAGTCTCCA 
CAAGATAGCC AGGTTCCTTC CGTTCATGTA GAAGTGCATC TTCCTAGGGA CCAGTCTGGG 
GAAGTGGACT TCCAGTCATT GGTTTCACAG TTGAAGGAGA CCTCAAGCTT ACAGGAGCAA 
GCTGATATAC TCTACATGCT GTATTCAATG AAGGTGATAC AGGAAAAAGC CTCATGGGGA 
GCCACCCTCT TTCAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phka1 ( XM_006527894.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527957.1 Mus musculus phosphorylase kinase alpha 1 (Phka1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu56199
Clone ID Related Accession (Same CDS sequence)	XM_006527894.3 , XM_006527894.4
Accession Version	XM_006527894.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527894.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCC GCAGTAACTC GGGAGTCCGC CTGGACGGCT ATGCTCGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACA GGCTTGCTTC CAGCCAGCTA TGATCAAAAA 
GATGCCTGGG TCCGAGATAA TGTGTACAGT ATCCTGGCTG TGTGGGGTTT GGGCCTGGCA 
TATCGCAAAA ATGCTGACCG TGATGAGGAC AAGGCAAAGG CCTATGAACT GGAGCAGAGT 
GTAGTGAAGT TAATGAGGGG ACTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAG 
TCCTTCAAGT ACAGTCAGAG TACTAAAGAT AGCCTCCATG CCAAGTACAA CACCAAAACT 
TGTGCCACTG TGGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACTTCTGTG 
TACCTGCTCT TCCTAGCACA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAC 
GAAGTCAATT TTATACAGAA CCTCGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTTGGGATAT GGGAACGTGG CGATAAGACA AACCAAGGCA TCTCGGAATT GAATGCGAGT 
TCAGTTGGAA TGGCCAAGGC AGCCCTGGAA GCACTAGATG AATTAGACTT GTTTGGTGTG 
AAAGGTGGGC CACAATCAGT TATCCATGTC CTGGCTGATG AAGTCCAACA CTGCCAGTCT 
ATCCTGAATT CACTACTGCC CAGGGCTTCA ACATCCAAAG AAGTTGATGC CAGTCTGCTC 
TCAGTGGTCT CTTTCCCAGC CTTTGCTGTA GAGGACAGCC ATTTGGTGGA GCTCACCAAA 
CAGGAGATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGTTTTCT GCGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGC CTATACTATG AACCAGCTGA GCTGAAGCTA 
TTTGAAAACA TTGAGTGCGA ATGGCCATTG TTCTGGACAT ACTTTATCCT TGATGGGATC 
TTCAGTGGCA ACGTAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGATGC AGTCCTCATC 
AAGGGCAAAA ATGGAGTCCC TCTTCTTCCA GAGCTGTACA GTGTCCCTCC TGACAGGGTT 
GATGAAGAGT ATCAAAATCC CCACACTGTG GATCGAGTCC CTATGGGAAA ATTGCCTCAC 
ATGTGGGGTC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTGAA TCGTAGGTTT TCTACTGTGC CAAAGCCAGA TGTGGTGGTT 
CAAGTCTCCA TTCTGGCTGA AACAGAAGAA ATCAAGGCCA TTTTGAAGGA CAAAGGAATT 
GATGTGGAGA CCATTGCTGA AGTGTACCCC ATAAGAGTAC AGCCAGCTCG TATTCTCAGC 
CATATTTATT CTAGTCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG 
CTCATGGGTG TGCTTGGAAC ATCAAAACTT TATGACATTC GCAAAACTAT CTTTACTTTC 
ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACCA GATGATAGTA 
GAAATGCTCA GAACAGACCT TTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC 
ACGATCACTT TCCCTATCTC GCACACCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCTTGG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGGGC CAGGATCCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGCTGCA CACACTTAAG CTTCATGGAC 
CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTATGATG AAGACTATGA AGATGATTTG 
GACTCTGGCA ACTGGATGGA CAGCTATGAT TCAACAAGTA ATGCTCGCTG TGGTGATGAA 
GTTGCCCGTT ATTTAGACCG CCTTTTGGCA CACACTGTTC CCCATCCTAA ACTAGCTCCT 
ACCTCACGGA AGGGAGGGCT AGATCGGTTC CGAGCTGCTG TGCAAACAAC TTGCGACTTA 
ATGTCCTTGG TGGCCAAGGC CAAGGAGCTG CATATACAGA ATGTTCACAT GTATCTTCCT 
ACAAAGTTAT TTCAGCCTTC TCGTCCTTCA CTCAACTTAC TTGACTCCCC TGAGTCTCCA 
CAAGATAGCC AGGTTCCTTC CGTTCATGTA GAAGTGCATC TTCCTAGGGA CCAGTCTGGG 
GAAGTGGACT TCCAGTCATT GGTTTCACAG TTGAAGGAGA CCTCAAGCTT ACAGGAGCAA 
GCTGATATAC TCTACATGCT GTATTCAATG AAAGGACCTG ACTGGAACAC TGAATTGTAT 
GAAGAAGGGG GGGCTACTGT CAGAGAGCTT CTTAGTGAAC TGTATGTCAA AGTTGGTGAA 
ATTCGGCACT GGGGTCTGAT CCGATATATC TCTGGGATCT TACGGAAGAA AGTGGAGGCA 
CTTGATGAGG CCTGCACAGA CCTTCTGTCC TACCAGAAAC ACCTGACAGT AGGACTTCCT 
CCAGAACCTC GAGAGAAGAC CATCTCTGCG CCTCTACCGT ACGAGGCACT CACTAAGCTG 
ATAGATGAAG CCAGTGAAGG CGACATGAGC ATCTCAACCC TTACACAGGA AATAATGGTC 
TATCTTGCCA TGTATATGAG AACTCAGCCT GGCCTCTTTG CAGAAATGTT CAGACTTCGA 
ATCGGTTTGA TCATACAAGT TATGGCAACA GAACTAGCAC ACTCTCTTCG ATGTTCAGCT 
GAGGAAGCCA CAGAGGGCCT GATGAATCTC AGTCCTTCAG CCATGAAGAA CCTCCTGCAT 
CACATTCTCA GTGGCAAAGA GTTTGGAGTG GAACGAAGCG TTCGTCCCAC TGATTCAAAT 
GTGAGTCCTG CTATTTCCAT CCATGAGATT GGTGCTGTTG GAGCAACAAA AACTGAACGA 
ACTGGAATCA TGCAGTTAAA AAGTGAGATA AAGCAGACTA GTGGTGGTCA TCCCTCGGGT 
GTAGATTTGA TGTCGCCATC CTTTCTGTCC CCTGCAGCCT GTATTGCTGC AAGTAGTGGA 
TCCTTTCCTA CGGTGTGTGA TCATCAGACA TCTAAAGATA GTCGTCAAGG CCAGTGGCAA 
CGCAGGAGAA GGCTAGATGG AGCACTAAAT AGAGTACCAA TTGGATTTTA TCAAAAAGTA 
TGGAAAATTT TGCAGAAATG TCATGGGCTT TCTGTGGAAG GTTTTGTTCT TCCCTCTTCA 
ACCACTAGGG AGATGACCCC AGGTGAGATT AAATTCTCTG TCCATGTGGA GTCTGTCCTG 
AATCGTGTCC CTCAGCCAGA ATACCGCCAA CTTCTGGTTG AAGCCATCCT TGTTCTCACC 
ATGCTGGCAG ATATTGAAAT TCATAGCATT GGGAGCATCA TTGCTGTGGA GAAAATAGTT 
CATATTGCCA ACGACTTGTT CCTTCAAGAA CAGAAAACCC TCGGCGCAGA TGATACCATG 
TTGGCAAAGG ATCCTGCATC TGGCATCTGT ACTCTCCTGT ATGACAGTGC ACCCAGTGGC 
AGATTTGGCA CCATGACCTA CCTCTCCAAG GCAGCTGCCA CCTACGTGCA GGAGTTTCTG 
CCACACAGCC TCTGTGCCAT GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527957.1
CDS	378..4064
Translation	MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSLLPRASTSKEVDASLLSVVSFPAFAVEDSHLVELTKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGIFSGNVEQVQEYREALDAVLIKGKNGVPLLPELYSVPPDRVDEEYQNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKAILKDKGIDVETIAEVYPIRVQPARILSHIYSSLGCNSRMKLSGRPYRLMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNQMIVEMLRTDLSYLCSRWRMTGQPTITFPISHTMLDEDGTSLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDEDYEDDLDSGNWMDSYDSTSNARCGDEVARYLDRLLAHTVPHPKLAPTSRKGGLDRFRAAVQTTCDLMSLVAKAKELHIQNVHMYLPTKLFQPSRPSLNLLDSPESPQDSQVPSVHVEVHLPRDQSGEVDFQSLVSQLKETSSLQEQADILYMLYSMKGPDWNTELYEEGGATVRELLSELYVKVGEIRHWGLIRYISGILRKKVEALDEACTDLLSYQKHLTVGLPPEPREKTISAPLPYEALTKLIDEASEGDMSISTLTQEIMVYLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATEGLMNLSPSAMKNLLHHILSGKEFGVERSVRPTDSNVSPAISIHEIGAVGATKTERTGIMQLKSEIKQTSGGHPSGVDLMSPSFLSPAACIAASSGSFPTVCDHQTSKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKILQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMLADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDTMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSLCAMQ

Target ORF information:

RefSeq Version	XM_006527894.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphorylase kinase alpha 1 (Phka1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527894.4

ORF Insert Sequence:

ATGAGGAGCC GCAGTAACTC GGGAGTCCGC CTGGACGGCT ATGCTCGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACA GGCTTGCTTC CAGCCAGCTA TGATCAAAAA 
GATGCCTGGG TCCGAGATAA TGTGTACAGT ATCCTGGCTG TGTGGGGTTT GGGCCTGGCA 
TATCGCAAAA ATGCTGACCG TGATGAGGAC AAGGCAAAGG CCTATGAACT GGAGCAGAGT 
GTAGTGAAGT TAATGAGGGG ACTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAG 
TCCTTCAAGT ACAGTCAGAG TACTAAAGAT AGCCTCCATG CCAAGTACAA CACCAAAACT 
TGTGCCACTG TGGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACTTCTGTG 
TACCTGCTCT TCCTAGCACA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAC 
GAAGTCAATT TTATACAGAA CCTCGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTTGGGATAT GGGAACGTGG CGATAAGACA AACCAAGGCA TCTCGGAATT GAATGCGAGT 
TCAGTTGGAA TGGCCAAGGC AGCCCTGGAA GCACTAGATG AATTAGACTT GTTTGGTGTG 
AAAGGTGGGC CACAATCAGT TATCCATGTC CTGGCTGATG AAGTCCAACA CTGCCAGTCT 
ATCCTGAATT CACTACTGCC CAGGGCTTCA ACATCCAAAG AAGTTGATGC CAGTCTGCTC 
TCAGTGGTCT CTTTCCCAGC CTTTGCTGTA GAGGACAGCC ATTTGGTGGA GCTCACCAAA 
CAGGAGATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGTTTTCT GCGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGC CTATACTATG AACCAGCTGA GCTGAAGCTA 
TTTGAAAACA TTGAGTGCGA ATGGCCATTG TTCTGGACAT ACTTTATCCT TGATGGGATC 
TTCAGTGGCA ACGTAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGATGC AGTCCTCATC 
AAGGGCAAAA ATGGAGTCCC TCTTCTTCCA GAGCTGTACA GTGTCCCTCC TGACAGGGTT 
GATGAAGAGT ATCAAAATCC CCACACTGTG GATCGAGTCC CTATGGGAAA ATTGCCTCAC 
ATGTGGGGTC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTGAA TCGTAGGTTT TCTACTGTGC CAAAGCCAGA TGTGGTGGTT 
CAAGTCTCCA TTCTGGCTGA AACAGAAGAA ATCAAGGCCA TTTTGAAGGA CAAAGGAATT 
GATGTGGAGA CCATTGCTGA AGTGTACCCC ATAAGAGTAC AGCCAGCTCG TATTCTCAGC 
CATATTTATT CTAGTCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG 
CTCATGGGTG TGCTTGGAAC ATCAAAACTT TATGACATTC GCAAAACTAT CTTTACTTTC 
ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACCA GATGATAGTA 
GAAATGCTCA GAACAGACCT TTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC 
ACGATCACTT TCCCTATCTC GCACACCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCTTGG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGGGC CAGGATCCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGCTGCA CACACTTAAG CTTCATGGAC 
CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTATGATG AAGACTATGA AGATGATTTG 
GACTCTGGCA ACTGGATGGA CAGCTATGAT TCAACAAGTA ATGCTCGCTG TGGTGATGAA 
GTTGCCCGTT ATTTAGACCG CCTTTTGGCA CACACTGTTC CCCATCCTAA ACTAGCTCCT 
ACCTCACGGA AGGGAGGGCT AGATCGGTTC CGAGCTGCTG TGCAAACAAC TTGCGACTTA 
ATGTCCTTGG TGGCCAAGGC CAAGGAGCTG CATATACAGA ATGTTCACAT GTATCTTCCT 
ACAAAGTTAT TTCAGCCTTC TCGTCCTTCA CTCAACTTAC TTGACTCCCC TGAGTCTCCA 
CAAGATAGCC AGGTTCCTTC CGTTCATGTA GAAGTGCATC TTCCTAGGGA CCAGTCTGGG 
GAAGTGGACT TCCAGTCATT GGTTTCACAG TTGAAGGAGA CCTCAAGCTT ACAGGAGCAA 
GCTGATATAC TCTACATGCT GTATTCAATG AAAGGACCTG ACTGGAACAC TGAATTGTAT 
GAAGAAGGGG GGGCTACTGT CAGAGAGCTT CTTAGTGAAC TGTATGTCAA AGTTGGTGAA 
ATTCGGCACT GGGGTCTGAT CCGATATATC TCTGGGATCT TACGGAAGAA AGTGGAGGCA 
CTTGATGAGG CCTGCACAGA CCTTCTGTCC TACCAGAAAC ACCTGACAGT AGGACTTCCT 
CCAGAACCTC GAGAGAAGAC CATCTCTGCG CCTCTACCGT ACGAGGCACT CACTAAGCTG 
ATAGATGAAG CCAGTGAAGG CGACATGAGC ATCTCAACCC TTACACAGGA AATAATGGTC 
TATCTTGCCA TGTATATGAG AACTCAGCCT GGCCTCTTTG CAGAAATGTT CAGACTTCGA 
ATCGGTTTGA TCATACAAGT TATGGCAACA GAACTAGCAC ACTCTCTTCG ATGTTCAGCT 
GAGGAAGCCA CAGAGGGCCT GATGAATCTC AGTCCTTCAG CCATGAAGAA CCTCCTGCAT 
CACATTCTCA GTGGCAAAGA GTTTGGAGTG GAACGAAGCG TTCGTCCCAC TGATTCAAAT 
GTGAGTCCTG CTATTTCCAT CCATGAGATT GGTGCTGTTG GAGCAACAAA AACTGAACGA 
ACTGGAATCA TGCAGTTAAA AAGTGAGATA AAGCAGACTA GTGGTGGTCA TCCCTCGGGT 
GTAGATTTGA TGTCGCCATC CTTTCTGTCC CCTGCAGCCT GTATTGCTGC AAGTAGTGGA 
TCCTTTCCTA CGGTGTGTGA TCATCAGACA TCTAAAGATA GTCGTCAAGG CCAGTGGCAA 
CGCAGGAGAA GGCTAGATGG AGCACTAAAT AGAGTACCAA TTGGATTTTA TCAAAAAGTA 
TGGAAAATTT TGCAGAAATG TCATGGGCTT TCTGTGGAAG GTTTTGTTCT TCCCTCTTCA 
ACCACTAGGG AGATGACCCC AGGTGAGATT AAATTCTCTG TCCATGTGGA GTCTGTCCTG 
AATCGTGTCC CTCAGCCAGA ATACCGCCAA CTTCTGGTTG AAGCCATCCT TGTTCTCACC 
ATGCTGGCAG ATATTGAAAT TCATAGCATT GGGAGCATCA TTGCTGTGGA GAAAATAGTT 
CATATTGCCA ACGACTTGTT CCTTCAAGAA CAGAAAACCC TCGGCGCAGA TGATACCATG 
TTGGCAAAGG ATCCTGCATC TGGCATCTGT ACTCTCCTGT ATGACAGTGC ACCCAGTGGC 
AGATTTGGCA CCATGACCTA CCTCTCCAAG GCAGCTGCCA CCTACGTGCA GGAGTTTCTG 
CCACACAGCC TCTGTGCCAT GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phka1 ( XM_006527895.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527958.1 Mus musculus phosphorylase kinase alpha 1 (Phka1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu56200
Clone ID Related Accession (Same CDS sequence)	XM_006527895.3 , XM_006527895.4
Accession Version	XM_006527895.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006527895.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCC GCAGTAACTC GGGAGTCCGC CTGGACGGCT ATGCTCGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACA GGCTTGCTTC CAGCCAGCTA TGATCAAAAA 
GATGCCTGGG TCCGAGATAA TGTGTACAGT ATCCTGGCTG TGTGGGGTTT GGGCCTGGCA 
TATCGCAAAA ATGCTGACCG TGATGAGGAC AAGGCAAAGG CCTATGAACT GGAGCAGAGT 
GTAGTGAAGT TAATGAGGGG ACTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAG 
TCCTTCAAGT ACAGTCAGAG TACTAAAGAT AGCCTCCATG CCAAGTACAA CACCAAAACT 
TGTGCCACTG TGGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACTTCTGTG 
TACCTGCTCT TCCTAGCACA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAC 
GAAGTCAATT TTATACAGAA CCTCGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTTGGGATAT GGGAACGTGG CGATAAGACA AACCAAGGCA TCTCGGAATT GAATGCGAGT 
TCAGTTGGAA TGGCCAAGGC AGCCCTGGAA GCACTAGATG AATTAGACTT GTTTGGTGTG 
AAAGGTGGGC CACAATCAGT TATCCATGTC CTGGCTGATG AAGTCCAACA CTGCCAGTCT 
ATCCTGAATT CACTACTGCC CAGGGCTTCA ACATCCAAAG AAGTTGATGC CAGTCTGCTC 
TCAGTGGTCT CTTTCCCAGC CTTTGCTGTA GAGGACAGCC ATTTGGTGGA GCTCACCAAA 
CAGGAGATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGTTTTCT GCGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGC CTATACTATG AACCAGCTGA GCTGAAGCTA 
TTTGAAAACA TTGAGTGCGA ATGGCCATTG TTCTGGACAT ACTTTATCCT TGATGGGATC 
TTCAGTGGCA ACGTAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGATGC AGTCCTCATC 
AAGGGCAAAA ATGGAGTCCC TCTTCTTCCA GAGCTGTACA GTGTCCCTCC TGACAGGGTT 
GATGAAGAGT ATCAAAATCC CCACACTGTG GATCGAGTCC CTATGGGAAA ATTGCCTCAC 
ATGTGGGGTC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTGAA TCGTAGGTTT TCTACTGTGC CAAAGCCAGA TGTGGTGGTT 
CAAGTCTCCA TTCTGGCTGA AACAGAAGAA ATCAAGGCCA TTTTGAAGGA CAAAGGAATT 
GATGTGGAGA CCATTGCTGA AGTGTACCCC ATAAGAGTAC AGCCAGCTCG TATTCTCAGC 
CATATTTATT CTAGTCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG 
CTCATGGGTG TGCTTGGAAC ATCAAAACTT TATGACATTC GCAAAACTAT CTTTACTTTC 
ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACCA GATGATAGTA 
GAAATGCTCA GAACAGACCT TTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC 
ACGATCACTT TCCCTATCTC GCACACCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCTTGG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGGGC CAGGATCCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGCTGCA CACACTTAAG CTTCATGGAC 
CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTATGATG AAGACTATGA AGATGATTTG 
GACTCTGGCA ACTGGATGGA CAGCTATGAT TCAACAAGTA ATGCTCGCTG TGGTGATGAA 
GTTGCCCGTT ATTTAGACCG CCTTTTGGCA CACACTGTTC CCCATCCTAA ACTAGCTCCT 
ACCTCACGGA AGGGAGGGCT AGATCGGTTC CGAGCTGCTG TGCAAACAAC TTGCGACTTA 
ATGTCCTTGG TGGCCAAGGC CAAGGAGCTG CATATACAGA ATGTTCACAT GTATCTTCCT 
ACAAAGTTAT TTCAGCCTTC TCGTCCTTCA CTCAACTTAC TTGACTCCCC TGAGTCTCCA 
CAAGATAGCC AGGTTCCTTC CGTTCATGTA GAAGTGCATC TTCCTAGGGA CCAGTCTGGG 
GAAGTGGACT TCCAGTCATT GGTTTCACAG TTGAAGGAGA CCTCAAGCTT ACAGGAGCAA 
GCTGATATAC TCTACATGCT GTATTCAATG AAGCCTCTAC CGTACGAGGC ACTCACTAAG 
CTGATAGATG AAGCCAGTGA AGGCGACATG AGCATCTCAA CCCTTACACA GGAAATAATG 
GTCTATCTTG CCATGTATAT GAGAACTCAG CCTGGCCTCT TTGCAGAAAT GTTCAGACTT 
CGAATCGGTT TGATCATACA AGTTATGGCA ACAGAACTAG CACACTCTCT TCGATGTTCA 
GCTGAGGAAG CCACAGAGGG CCTGATGAAT CTCAGTCCTT CAGCCATGAA GAACCTCCTG 
CATCACATTC TCAGTGGCAA AGAGTTTGGA GTGGAACGAA GCGTTCGTCC CACTGATTCA 
AATGTGAGTC CTGCTATTTC CATCCATGAG ATTGGTGCTG TTGGAGCAAC AAAAACTGAA 
CGAACTGGAA TCATGCAGTT AAAAAGTGAG ATAAAGCAGG TGGAATTTCG TAGGCTGTCT 
GTCTCGATGG AGAGTCAGAC TAGTGGTGGT CATCCCTCGG GTGTAGATTT GATGTCGCCA 
TCCTTTCTGT CCCCTGCAGC CTGTATTGCT GCAAGTAGTG GATCCTTTCC TACGGTGTGT 
GATCATCAGA CATCTAAAGA TAGTCGTCAA GGCCAGTGGC AACGCAGGAG AAGGCTAGAT 
GGAGCACTAA ATAGAGTACC AATTGGATTT TATCAAAAAG TATGGAAAAT TTTGCAGAAA 
TGTCATGGGC TTTCTGTGGA AGGTTTTGTT CTTCCCTCTT CAACCACTAG GGAGATGACC 
CCAGGTGAGA TTAAATTCTC TGTCCATGTG GAGTCTGTCC TGAATCGTGT CCCTCAGCCA 
GAATACCGCC AACTTCTGGT TGAAGCCATC CTTGTTCTCA CCATGCTGGC AGATATTGAA 
ATTCATAGCA TTGGGAGCAT CATTGCTGTG GAGAAAATAG TTCATATTGC CAACGACTTG 
TTCCTTCAAG AACAGAAAAC CCTCGGCGCA GATGATACCA TGTTGGCAAA GGATCCTGCA 
TCTGGCATCT GTACTCTCCT GTATGACAGT GCACCCAGTG GCAGATTTGG CACCATGACC 
TACCTCTCCA AGGCAGCTGC CACCTACGTG CAGGAGTTTC TGCCACACAG CCTCTGTGCC 
ATGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527958.1
CDS	378..3866
Translation	MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSLLPRASTSKEVDASLLSVVSFPAFAVEDSHLVELTKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGIFSGNVEQVQEYREALDAVLIKGKNGVPLLPELYSVPPDRVDEEYQNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKAILKDKGIDVETIAEVYPIRVQPARILSHIYSSLGCNSRMKLSGRPYRLMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNQMIVEMLRTDLSYLCSRWRMTGQPTITFPISHTMLDEDGTSLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDEDYEDDLDSGNWMDSYDSTSNARCGDEVARYLDRLLAHTVPHPKLAPTSRKGGLDRFRAAVQTTCDLMSLVAKAKELHIQNVHMYLPTKLFQPSRPSLNLLDSPESPQDSQVPSVHVEVHLPRDQSGEVDFQSLVSQLKETSSLQEQADILYMLYSMKPLPYEALTKLIDEASEGDMSISTLTQEIMVYLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATEGLMNLSPSAMKNLLHHILSGKEFGVERSVRPTDSNVSPAISIHEIGAVGATKTERTGIMQLKSEIKQVEFRRLSVSMESQTSGGHPSGVDLMSPSFLSPAACIAASSGSFPTVCDHQTSKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKILQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMLADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDTMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSLCAMQ

Target ORF information:

RefSeq Version	XM_006527895.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphorylase kinase alpha 1 (Phka1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527895.4

ORF Insert Sequence:

ATGAGGAGCC GCAGTAACTC GGGAGTCCGC CTGGACGGCT ATGCTCGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACA GGCTTGCTTC CAGCCAGCTA TGATCAAAAA 
GATGCCTGGG TCCGAGATAA TGTGTACAGT ATCCTGGCTG TGTGGGGTTT GGGCCTGGCA 
TATCGCAAAA ATGCTGACCG TGATGAGGAC AAGGCAAAGG CCTATGAACT GGAGCAGAGT 
GTAGTGAAGT TAATGAGGGG ACTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAG 
TCCTTCAAGT ACAGTCAGAG TACTAAAGAT AGCCTCCATG CCAAGTACAA CACCAAAACT 
TGTGCCACTG TGGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACTTCTGTG 
TACCTGCTCT TCCTAGCACA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAC 
GAAGTCAATT TTATACAGAA CCTCGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTTGGGATAT GGGAACGTGG CGATAAGACA AACCAAGGCA TCTCGGAATT GAATGCGAGT 
TCAGTTGGAA TGGCCAAGGC AGCCCTGGAA GCACTAGATG AATTAGACTT GTTTGGTGTG 
AAAGGTGGGC CACAATCAGT TATCCATGTC CTGGCTGATG AAGTCCAACA CTGCCAGTCT 
ATCCTGAATT CACTACTGCC CAGGGCTTCA ACATCCAAAG AAGTTGATGC CAGTCTGCTC 
TCAGTGGTCT CTTTCCCAGC CTTTGCTGTA GAGGACAGCC ATTTGGTGGA GCTCACCAAA 
CAGGAGATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGTTTTCT GCGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGC CTATACTATG AACCAGCTGA GCTGAAGCTA 
TTTGAAAACA TTGAGTGCGA ATGGCCATTG TTCTGGACAT ACTTTATCCT TGATGGGATC 
TTCAGTGGCA ACGTAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGATGC AGTCCTCATC 
AAGGGCAAAA ATGGAGTCCC TCTTCTTCCA GAGCTGTACA GTGTCCCTCC TGACAGGGTT 
GATGAAGAGT ATCAAAATCC CCACACTGTG GATCGAGTCC CTATGGGAAA ATTGCCTCAC 
ATGTGGGGTC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTGAA TCGTAGGTTT TCTACTGTGC CAAAGCCAGA TGTGGTGGTT 
CAAGTCTCCA TTCTGGCTGA AACAGAAGAA ATCAAGGCCA TTTTGAAGGA CAAAGGAATT 
GATGTGGAGA CCATTGCTGA AGTGTACCCC ATAAGAGTAC AGCCAGCTCG TATTCTCAGC 
CATATTTATT CTAGTCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG 
CTCATGGGTG TGCTTGGAAC ATCAAAACTT TATGACATTC GCAAAACTAT CTTTACTTTC 
ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACCA GATGATAGTA 
GAAATGCTCA GAACAGACCT TTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC 
ACGATCACTT TCCCTATCTC GCACACCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCTTGG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGGGC CAGGATCCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGCTGCA CACACTTAAG CTTCATGGAC 
CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTATGATG AAGACTATGA AGATGATTTG 
GACTCTGGCA ACTGGATGGA CAGCTATGAT TCAACAAGTA ATGCTCGCTG TGGTGATGAA 
GTTGCCCGTT ATTTAGACCG CCTTTTGGCA CACACTGTTC CCCATCCTAA ACTAGCTCCT 
ACCTCACGGA AGGGAGGGCT AGATCGGTTC CGAGCTGCTG TGCAAACAAC TTGCGACTTA 
ATGTCCTTGG TGGCCAAGGC CAAGGAGCTG CATATACAGA ATGTTCACAT GTATCTTCCT 
ACAAAGTTAT TTCAGCCTTC TCGTCCTTCA CTCAACTTAC TTGACTCCCC TGAGTCTCCA 
CAAGATAGCC AGGTTCCTTC CGTTCATGTA GAAGTGCATC TTCCTAGGGA CCAGTCTGGG 
GAAGTGGACT TCCAGTCATT GGTTTCACAG TTGAAGGAGA CCTCAAGCTT ACAGGAGCAA 
GCTGATATAC TCTACATGCT GTATTCAATG AAGCCTCTAC CGTACGAGGC ACTCACTAAG 
CTGATAGATG AAGCCAGTGA AGGCGACATG AGCATCTCAA CCCTTACACA GGAAATAATG 
GTCTATCTTG CCATGTATAT GAGAACTCAG CCTGGCCTCT TTGCAGAAAT GTTCAGACTT 
CGAATCGGTT TGATCATACA AGTTATGGCA ACAGAACTAG CACACTCTCT TCGATGTTCA 
GCTGAGGAAG CCACAGAGGG CCTGATGAAT CTCAGTCCTT CAGCCATGAA GAACCTCCTG 
CATCACATTC TCAGTGGCAA AGAGTTTGGA GTGGAACGAA GCGTTCGTCC CACTGATTCA 
AATGTGAGTC CTGCTATTTC CATCCATGAG ATTGGTGCTG TTGGAGCAAC AAAAACTGAA 
CGAACTGGAA TCATGCAGTT AAAAAGTGAG ATAAAGCAGG TGGAATTTCG TAGGCTGTCT 
GTCTCGATGG AGAGTCAGAC TAGTGGTGGT CATCCCTCGG GTGTAGATTT GATGTCGCCA 
TCCTTTCTGT CCCCTGCAGC CTGTATTGCT GCAAGTAGTG GATCCTTTCC TACGGTGTGT 
GATCATCAGA CATCTAAAGA TAGTCGTCAA GGCCAGTGGC AACGCAGGAG AAGGCTAGAT 
GGAGCACTAA ATAGAGTACC AATTGGATTT TATCAAAAAG TATGGAAAAT TTTGCAGAAA 
TGTCATGGGC TTTCTGTGGA AGGTTTTGTT CTTCCCTCTT CAACCACTAG GGAGATGACC 
CCAGGTGAGA TTAAATTCTC TGTCCATGTG GAGTCTGTCC TGAATCGTGT CCCTCAGCCA 
GAATACCGCC AACTTCTGGT TGAAGCCATC CTTGTTCTCA CCATGCTGGC AGATATTGAA 
ATTCATAGCA TTGGGAGCAT CATTGCTGTG GAGAAAATAG TTCATATTGC CAACGACTTG 
TTCCTTCAAG AACAGAAAAC CCTCGGCGCA GATGATACCA TGTTGGCAAA GGATCCTGCA 
TCTGGCATCT GTACTCTCCT GTATGACAGT GCACCCAGTG GCAGATTTGG CACCATGACC 
TACCTCTCCA AGGCAGCTGC CACCTACGTG CAGGAGTTTC TGCCACACAG CCTCTGTGCC 
ATGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phka1 ( NM_008832.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032858.2 Mus musculus phosphorylase kinase alpha 1 (Phka1), transcript variant 1, mRNA.

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CloneID	OMu23486
Clone ID Related Accession (Same CDS sequence)	NM_008832.2
Accession Version	NM_008832.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-23
Organism	Mus musculus(house mouse)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL732405.8 and AL805925.6. On Jul 26, 2007 this sequence version replaced NM_008832.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X74616.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGAGGAGCC GCAGTAACTC GGGAGTCCGC CTGGACGGCT ATGCTCGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACA GGCTTGCTTC CAGCCAGCTA TGATCAAAAA 
GATGCCTGGG TCCGAGATAA TGTGTACAGT ATCCTGGCTG TGTGGGGTTT GGGCCTGGCA 
TATCGCAAAA ATGCTGACCG TGATGAGGAC AAGGCAAAGG CCTATGAACT GGAGCAGAGT 
GTAGTGAAGT TAATGAGGGG ACTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAG 
TCCTTCAAGT ACAGTCAGAG TACTAAAGAT AGCCTCCATG CCAAGTACAA CACCAAAACT 
TGTGCCACTG TGGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACTTCTGTG 
TACCTGCTCT TCCTAGCACA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAC 
GAAGTCAATT TTATACAGAA CCTCGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTTGGGATAT GGGAACGTGG CGATAAGACA AACCAAGGCA TCTCGGAATT GAATGCGAGT 
TCAGTTGGAA TGGCCAAGGC AGCCCTGGAA GCACTAGATG AATTAGACTT GTTTGGTGTG 
AAAGGTGGGC CACAATCAGT TATCCATGTC CTGGCTGATG AAGTCCAACA CTGCCAGTCT 
ATCCTGAATT CACTACTGCC CAGGGCTTCA ACATCCAAAG AAGTTGATGC CAGTCTGCTC 
TCAGTGGTCT CTTTCCCAGC CTTTGCTGTA GAGGACAGCC ATTTGGTGGA GCTCACCAAA 
CAGGAGATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGTTTTCT GCGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGC CTATACTATG AACCAGCTGA GCTGAAGCTA 
TTTGAAAACA TTGAGTGCGA ATGGCCATTG TTCTGGACAT ACTTTATCCT TGATGGGATC 
TTCAGTGGCA ACGTAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGATGC AGTCCTCATC 
AAGGGCAAAA ATGGAGTCCC TCTTCTTCCA GAGCTGTACA GTGTCCCTCC TGACAGGGTT 
GATGAAGAGT ATCAAAATCC CCACACTGTG GATCGAGTCC CTATGGGAAA ATTGCCTCAC 
ATGTGGGGTC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTGAA TCGTAGGTTT TCTACTGTGC CAAAGCCAGA TGTGGTGGTT 
CAAGTCTCCA TTCTGGCTGA AACAGAAGAA ATCAAGGCCA TTTTGAAGGA CAAAGGAATT 
GATGTGGAGA CCATTGCTGA AGTGTACCCC ATAAGAGTAC AGCCAGCTCG TATTCTCAGC 
CATATTTATT CTAGTCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG 
CTCATGGGTG TGCTTGGAAC ATCAAAACTT TATGACATTC GCAAAACTAT CTTTACTTTC 
ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACCA GATGATAGTA 
GAAATGCTCA GAACAGACCT TTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC 
ACGATCACTT TCCCTATCTC GCACACCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCTTGG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGGGC CAGGATCCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGCTGCA CACACTTAAG CTTCATGGAC 
CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTATGATG AAGACTATGA AGATGATTTG 
GACTCTGGCA ACTGGATGGA CAGCTATGAT TCAACAAGTA ATGCTCGCTG TGGTGATGAA 
GTTGCCCGTT ATTTAGACCG CCTTTTGGCA CACACTGTTC CCCATCCTAA ACTAGCTCCT 
ACCTCACGGA AGGGAGGGCT AGATCGGTTC CGAGCTGCTG TGCAAACAAC TTGCGACTTA 
ATGTCCTTGG TGGCCAAGGC CAAGGAGCTG CATATACAGA ATGTTCACAT GTATCTTCCT 
ACAAAGTTAT TTCAGCCTTC TCGTCCTTCA CTCAACTTAC TTGACTCCCC TGAGTCTCCA 
CAAGATAGCC AGGTTCCTTC CGTTCATGTA GAAGTGCATC TTCCTAGGGA CCAGTCTGGG 
GAAGTGGACT TCCAGTCATT GGTTTCACAG TTGAAGGAGA CCTCAAGCTT ACAGGAGCAA 
GCTGATATAC TCTACATGCT GTATTCAATG AAAGGACCTG ACTGGAACAC TGAATTGTAT 
GAAGAAGGGG GGGCTACTGT CAGAGAGCTT CTTAGTGAAC TGTATGTCAA AGTTGGTGAA 
ATTCGGCACT GGGGTCTGAT CCGATATATC TCTGGGATCT TACGGAAGAA AGTGGAGGCA 
CTTGATGAGG CCTGCACAGA CCTTCTGTCC TACCAGAAAC ACCTGACAGT AGGACTTCCT 
CCAGAACCTC GAGAGAAGAC CATCTCTGCG CCTCTACCGT ACGAGGCACT CACTAAGCTG 
ATAGATGAAG CCAGTGAAGG CGACATGAGC ATCTCAACCC TTACACAGGA AATAATGGTC 
TATCTTGCCA TGTATATGAG AACTCAGCCT GGCCTCTTTG CAGAAATGTT CAGACTTCGA 
ATCGGTTTGA TCATACAAGT TATGGCAACA GAACTAGCAC ACTCTCTTCG ATGTTCAGCT 
GAGGAAGCCA CAGAGGGCCT GATGAATCTC AGTCCTTCAG CCATGAAGAA CCTCCTGCAT 
CACATTCTCA GTGGCAAAGA GTTTGGAGTG GAACGAAGCG TTCGTCCCAC TGATTCAAAT 
GTGAGTCCTG CTATTTCCAT CCATGAGATT GGTGCTGTTG GAGCAACAAA AACTGAACGA 
ACTGGAATCA TGCAGTTAAA AAGTGAGATA AAGCAGGTGG AATTTCGTAG GCTGTCTGTC 
TCGATGGAGA GTCAGACTAG TGGTGGTCAT CCCTCGGGTG TAGATTTGAT GTCGCCATCC 
TTTCTGTCCC CTGCAGCCTG TATTGCTGCA AGTAGTGGAT CCTTTCCTAC GGTGTGTGAT 
CATCAGACAT CTAAAGATAG TCGTCAAGGC CAGTGGCAAC GCAGGAGAAG GCTAGATGGA 
GCACTAAATA GAGTACCAAT TGGATTTTAT CAAAAAGTAT GGAAAATTTT GCAGAAATGT 
CATGGGCTTT CTGTGGAAGG TTTTGTTCTT CCCTCTTCAA CCACTAGGGA GATGACCCCA 
GGTGAGATTA AATTCTCTGT CCATGTGGAG TCTGTCCTGA ATCGTGTCCC TCAGCCAGAA 
TACCGCCAAC TTCTGGTTGA AGCCATCCTT GTTCTCACCA TGCTGGCAGA TATTGAAATT 
CATAGCATTG GGAGCATCAT TGCTGTGGAG AAAATAGTTC ATATTGCCAA CGACTTGTTC 
CTTCAAGAAC AGAAAACCCT CGGCGCAGAT GATACCATGT TGGCAAAGGA TCCTGCATCT 
GGCATCTGTA CTCTCCTGTA TGACAGTGCA CCCAGTGGCA GATTTGGCAC CATGACCTAC 
CTCTCCAAGG CAGCTGCCAC CTACGTGCAG GAGTTTCTGC CACACAGCCT CTGTGCCATG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032858.2
CDS	323..4048
Translation	MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSLLPRASTSKEVDASLLSVVSFPAFAVEDSHLVELTKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGIFSGNVEQVQEYREALDAVLIKGKNGVPLLPELYSVPPDRVDEEYQNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKAILKDKGIDVETIAEVYPIRVQPARILSHIYSSLGCNSRMKLSGRPYRLMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNQMIVEMLRTDLSYLCSRWRMTGQPTITFPISHTMLDEDGTSLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDEDYEDDLDSGNWMDSYDSTSNARCGDEVARYLDRLLAHTVPHPKLAPTSRKGGLDRFRAAVQTTCDLMSLVAKAKELHIQNVHMYLPTKLFQPSRPSLNLLDSPESPQDSQVPSVHVEVHLPRDQSGEVDFQSLVSQLKETSSLQEQADILYMLYSMKGPDWNTELYEEGGATVRELLSELYVKVGEIRHWGLIRYISGILRKKVEALDEACTDLLSYQKHLTVGLPPEPREKTISAPLPYEALTKLIDEASEGDMSISTLTQEIMVYLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATEGLMNLSPSAMKNLLHHILSGKEFGVERSVRPTDSNVSPAISIHEIGAVGATKTERTGIMQLKSEIKQVEFRRLSVSMESQTSGGHPSGVDLMSPSFLSPAACIAASSGSFPTVCDHQTSKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKILQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMLADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDTMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSLCAMQ

Target ORF information:

RefSeq Version	NM_008832.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphorylase kinase alpha 1 (Phka1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008832.2

ORF Insert Sequence:

ATGAGGAGCC GCAGTAACTC GGGAGTCCGC CTGGACGGCT ATGCTCGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACA GGCTTGCTTC CAGCCAGCTA TGATCAAAAA 
GATGCCTGGG TCCGAGATAA TGTGTACAGT ATCCTGGCTG TGTGGGGTTT GGGCCTGGCA 
TATCGCAAAA ATGCTGACCG TGATGAGGAC AAGGCAAAGG CCTATGAACT GGAGCAGAGT 
GTAGTGAAGT TAATGAGGGG ACTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAG 
TCCTTCAAGT ACAGTCAGAG TACTAAAGAT AGCCTCCATG CCAAGTACAA CACCAAAACT 
TGTGCCACTG TGGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACTTCTGTG 
TACCTGCTCT TCCTAGCACA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAC 
GAAGTCAATT TTATACAGAA CCTCGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTTGGGATAT GGGAACGTGG CGATAAGACA AACCAAGGCA TCTCGGAATT GAATGCGAGT 
TCAGTTGGAA TGGCCAAGGC AGCCCTGGAA GCACTAGATG AATTAGACTT GTTTGGTGTG 
AAAGGTGGGC CACAATCAGT TATCCATGTC CTGGCTGATG AAGTCCAACA CTGCCAGTCT 
ATCCTGAATT CACTACTGCC CAGGGCTTCA ACATCCAAAG AAGTTGATGC CAGTCTGCTC 
TCAGTGGTCT CTTTCCCAGC CTTTGCTGTA GAGGACAGCC ATTTGGTGGA GCTCACCAAA 
CAGGAGATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGTTTTCT GCGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGC CTATACTATG AACCAGCTGA GCTGAAGCTA 
TTTGAAAACA TTGAGTGCGA ATGGCCATTG TTCTGGACAT ACTTTATCCT TGATGGGATC 
TTCAGTGGCA ACGTAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGATGC AGTCCTCATC 
AAGGGCAAAA ATGGAGTCCC TCTTCTTCCA GAGCTGTACA GTGTCCCTCC TGACAGGGTT 
GATGAAGAGT ATCAAAATCC CCACACTGTG GATCGAGTCC CTATGGGAAA ATTGCCTCAC 
ATGTGGGGTC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTGAA TCGTAGGTTT TCTACTGTGC CAAAGCCAGA TGTGGTGGTT 
CAAGTCTCCA TTCTGGCTGA AACAGAAGAA ATCAAGGCCA TTTTGAAGGA CAAAGGAATT 
GATGTGGAGA CCATTGCTGA AGTGTACCCC ATAAGAGTAC AGCCAGCTCG TATTCTCAGC 
CATATTTATT CTAGTCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG 
CTCATGGGTG TGCTTGGAAC ATCAAAACTT TATGACATTC GCAAAACTAT CTTTACTTTC 
ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACCA GATGATAGTA 
GAAATGCTCA GAACAGACCT TTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC 
ACGATCACTT TCCCTATCTC GCACACCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCTTGG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGGGC CAGGATCCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGCTGCA CACACTTAAG CTTCATGGAC 
CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTATGATG AAGACTATGA AGATGATTTG 
GACTCTGGCA ACTGGATGGA CAGCTATGAT TCAACAAGTA ATGCTCGCTG TGGTGATGAA 
GTTGCCCGTT ATTTAGACCG CCTTTTGGCA CACACTGTTC CCCATCCTAA ACTAGCTCCT 
ACCTCACGGA AGGGAGGGCT AGATCGGTTC CGAGCTGCTG TGCAAACAAC TTGCGACTTA 
ATGTCCTTGG TGGCCAAGGC CAAGGAGCTG CATATACAGA ATGTTCACAT GTATCTTCCT 
ACAAAGTTAT TTCAGCCTTC TCGTCCTTCA CTCAACTTAC TTGACTCCCC TGAGTCTCCA 
CAAGATAGCC AGGTTCCTTC CGTTCATGTA GAAGTGCATC TTCCTAGGGA CCAGTCTGGG 
GAAGTGGACT TCCAGTCATT GGTTTCACAG TTGAAGGAGA CCTCAAGCTT ACAGGAGCAA 
GCTGATATAC TCTACATGCT GTATTCAATG AAAGGACCTG ACTGGAACAC TGAATTGTAT 
GAAGAAGGGG GGGCTACTGT CAGAGAGCTT CTTAGTGAAC TGTATGTCAA AGTTGGTGAA 
ATTCGGCACT GGGGTCTGAT CCGATATATC TCTGGGATCT TACGGAAGAA AGTGGAGGCA 
CTTGATGAGG CCTGCACAGA CCTTCTGTCC TACCAGAAAC ACCTGACAGT AGGACTTCCT 
CCAGAACCTC GAGAGAAGAC CATCTCTGCG CCTCTACCGT ACGAGGCACT CACTAAGCTG 
ATAGATGAAG CCAGTGAAGG CGACATGAGC ATCTCAACCC TTACACAGGA AATAATGGTC 
TATCTTGCCA TGTATATGAG AACTCAGCCT GGCCTCTTTG CAGAAATGTT CAGACTTCGA 
ATCGGTTTGA TCATACAAGT TATGGCAACA GAACTAGCAC ACTCTCTTCG ATGTTCAGCT 
GAGGAAGCCA CAGAGGGCCT GATGAATCTC AGTCCTTCAG CCATGAAGAA CCTCCTGCAT 
CACATTCTCA GTGGCAAAGA GTTTGGAGTG GAACGAAGCG TTCGTCCCAC TGATTCAAAT 
GTGAGTCCTG CTATTTCCAT CCATGAGATT GGTGCTGTTG GAGCAACAAA AACTGAACGA 
ACTGGAATCA TGCAGTTAAA AAGTGAGATA AAGCAGGTGG AATTTCGTAG GCTGTCTGTC 
TCGATGGAGA GTCAGACTAG TGGTGGTCAT CCCTCGGGTG TAGATTTGAT GTCGCCATCC 
TTTCTGTCCC CTGCAGCCTG TATTGCTGCA AGTAGTGGAT CCTTTCCTAC GGTGTGTGAT 
CATCAGACAT CTAAAGATAG TCGTCAAGGC CAGTGGCAAC GCAGGAGAAG GCTAGATGGA 
GCACTAAATA GAGTACCAAT TGGATTTTAT CAAAAAGTAT GGAAAATTTT GCAGAAATGT 
CATGGGCTTT CTGTGGAAGG TTTTGTTCTT CCCTCTTCAA CCACTAGGGA GATGACCCCA 
GGTGAGATTA AATTCTCTGT CCATGTGGAG TCTGTCCTGA ATCGTGTCCC TCAGCCAGAA 
TACCGCCAAC TTCTGGTTGA AGCCATCCTT GTTCTCACCA TGCTGGCAGA TATTGAAATT 
CATAGCATTG GGAGCATCAT TGCTGTGGAG AAAATAGTTC ATATTGCCAA CGACTTGTTC 
CTTCAAGAAC AGAAAACCCT CGGCGCAGAT GATACCATGT TGGCAAAGGA TCCTGCATCT 
GGCATCTGTA CTCTCCTGTA TGACAGTGCA CCCAGTGGCA GATTTGGCAC CATGACCTAC 
CTCTCCAAGG CAGCTGCCAC CTACGTGCAG GAGTTTCTGC CACACAGCCT CTGTGCCATG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phka1 ( NM_173021.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_766609.2 Mus musculus phosphorylase kinase alpha 1 (Phka1), transcript variant 2, mRNA.

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CloneID	OMu23500
Clone ID Related Accession (Same CDS sequence)	NM_173021.3
Accession Version	NM_173021.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB662994.1, AK036440.1, AK137497.1, AK163548.1 and BY414985.1. On Jul 26, 2007 this sequence version replaced NM_173021.2. Transcript Variant: This variant (2) lacks an exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK137497.1, AK036440.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGAGGAGCC GCAGTAACTC GGGAGTCCGC CTGGACGGCT ATGCTCGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACA GGCTTGCTTC CAGCCAGCTA TGATCAAAAA 
GATGCCTGGG TCCGAGATAA TGTGTACAGT ATCCTGGCTG TGTGGGGTTT GGGCCTGGCA 
TATCGCAAAA ATGCTGACCG TGATGAGGAC AAGGCAAAGG CCTATGAACT GGAGCAGAGT 
GTAGTGAAGT TAATGAGGGG ACTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAG 
TCCTTCAAGT ACAGTCAGAG TACTAAAGAT AGCCTCCATG CCAAGTACAA CACCAAAACT 
TGTGCCACTG TGGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACTTCTGTG 
TACCTGCTCT TCCTAGCACA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAC 
GAAGTCAATT TTATACAGAA CCTCGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTTGGGATAT GGGAACGTGG CGATAAGACA AACCAAGGCA TCTCGGAATT GAATGCGAGT 
TCAGTTGGAA TGGCCAAGGC AGCCCTGGAA GCACTAGATG AATTAGACTT GTTTGGTGTG 
AAAGGTGGGC CACAATCAGT TATCCATGTC CTGGCTGATG AAGTCCAACA CTGCCAGTCT 
ATCCTGAATT CACTACTGCC CAGGGCTTCA ACATCCAAAG AAGTTGATGC CAGTCTGCTC 
TCAGTGGTCT CTTTCCCAGC CTTTGCTGTA GAGGACAGCC ATTTGGTGGA GCTCACCAAA 
CAGGAGATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGTTTTCT GCGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGC CTATACTATG AACCAGCTGA GCTGAAGCTA 
TTTGAAAACA TTGAGTGCGA ATGGCCATTG TTCTGGACAT ACTTTATCCT TGATGGGATC 
TTCAGTGGCA ACGTAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGATGC AGTCCTCATC 
AAGGGCAAAA ATGGAGTCCC TCTTCTTCCA GAGCTGTACA GTGTCCCTCC TGACAGGGTT 
GATGAAGAGT ATCAAAATCC CCACACTGTG GATCGAGTCC CTATGGGAAA ATTGCCTCAC 
ATGTGGGGTC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTGAA TCGTAGGTTT TCTACTGTGC CAAAGCCAGA TGTGGTGGTT 
CAAGTCTCCA TTCTGGCTGA AACAGAAGAA ATCAAGGCCA TTTTGAAGGA CAAAGGAATT 
GATGTGGAGA CCATTGCTGA AGTGTACCCC ATAAGAGTAC AGCCAGCTCG TATTCTCAGC 
CATATTTATT CTAGTCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG 
CTCATGGGTG TGCTTGGAAC ATCAAAACTT TATGACATTC GCAAAACTAT CTTTACTTTC 
ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACCA GATGATAGTA 
GAAATGCTCA GAACAGACCT TTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC 
ACGATCACTT TCCCTATCTC GCACACCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCTTGG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGGGC CAGGATCCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGCTGCA CACACTTAAG CTTCATGGAC 
CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTATGATG AAGACTATGA AGATGATTTG 
GACTCTGGCA ACTGGATGGA CAGCTATGAT TCAACAAGTA ATGATGTTCA CATGTATCTT 
CCTACAAAGT TATTTCAGCC TTCTCGTCCT TCACTCAACT TACTTGACTC CCCTGAGTCT 
CCACAAGATA GCCAGGTTCC TTCCGTTCAT GTAGAAGTGC ATCTTCCTAG GGACCAGTCT 
GGGGAAGTGG ACTTCCAGTC ATTGGTTTCA CAGTTGAAGG AGACCTCAAG CTTACAGGAG 
CAAGCTGATA TACTCTACAT GCTGTATTCA ATGAAAGGAC CTGACTGGAA CACTGAATTG 
TATGAAGAAG GGGGGGCTAC TGTCAGAGAG CTTCTTAGTG AACTGTATGT CAAAGTTGGT 
GAAATTCGGC ACTGGGGTCT GATCCGATAT ATCTCTGGGA TCTTACGGAA GAAAGTGGAG 
GCACTTGATG AGGCCTGCAC AGACCTTCTG TCCTACCAGA AACACCTGAC AGTAGGACTT 
CCTCCAGAAC CTCGAGAGAA GACCATCTCT GCGCCTCTAC CGTACGAGGC ACTCACTAAG 
CTGATAGATG AAGCCAGTGA AGGCGACATG AGCATCTCAA CCCTTACACA GGAAATAATG 
GTCTATCTTG CCATGTATAT GAGAACTCAG CCTGGCCTCT TTGCAGAAAT GTTCAGACTT 
CGAATCGGTT TGATCATACA AGTTATGGCA ACAGAACTAG CACACTCTCT TCGATGTTCA 
GCTGAGGAAG CCACAGAGGG CCTGATGAAT CTCAGTCCTT CAGCCATGAA GAACCTCCTG 
CATCACATTC TCAGTGGCAA AGAGTTTGGA GTGGAACGAA GCGTTCGTCC CACTGATTCA 
AATGTGAGTC CTGCTATTTC CATCCATGAG ATTGGTGCTG TTGGAGCAAC AAAAACTGAA 
CGAACTGGAA TCATGCAGTT AAAAAGTGAG ATAAAGCAGG TGGAATTTCG TAGGCTGTCT 
GTCTCGATGG AGAGTCAGAC TAGTGGTGGT CATCCCTCGG GTGTAGATTT GATGTCGCCA 
TCCTTTCTGT CCCCTGCAGC CTGTATTGCT GCAAGTAGTG GATCCTTTCC TACGGTGTGT 
GATCATCAGA CATCTAAAGA TAGTCGTCAA GGCCAGTGGC AACGCAGGAG AAGGCTAGAT 
GGAGCACTAA ATAGAGTACC AATTGGATTT TATCAAAAAG TATGGAAAAT TTTGCAGAAA 
TGTCATGGGC TTTCTGTGGA AGGTTTTGTT CTTCCCTCTT CAACCACTAG GGAGATGACC 
CCAGGTGAGA TTAAATTCTC TGTCCATGTG GAGTCTGTCC TGAATCGTGT CCCTCAGCCA 
GAATACCGCC AACTTCTGGT TGAAGCCATC CTTGTTCTCA CCATGCTGGC AGATATTGAA 
ATTCATAGCA TTGGGAGCAT CATTGCTGTG GAGAAAATAG TTCATATTGC CAACGACTTG 
TTCCTTCAAG AACAGAAAAC CCTCGGCGCA GATGATACCA TGTTGGCAAA GGATCCTGCA 
TCTGGCATCT GTACTCTCCT GTATGACAGT GCACCCAGTG GCAGATTTGG CACCATGACC 
TACCTCTCCA AGGCAGCTGC CACCTACGTG CAGGAGTTTC TGCCACACAG CCTCTGTGCC 
ATGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_766609.2
CDS	323..3871
Translation	MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSLLPRASTSKEVDASLLSVVSFPAFAVEDSHLVELTKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGIFSGNVEQVQEYREALDAVLIKGKNGVPLLPELYSVPPDRVDEEYQNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKAILKDKGIDVETIAEVYPIRVQPARILSHIYSSLGCNSRMKLSGRPYRLMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNQMIVEMLRTDLSYLCSRWRMTGQPTITFPISHTMLDEDGTSLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDEDYEDDLDSGNWMDSYDSTSNDVHMYLPTKLFQPSRPSLNLLDSPESPQDSQVPSVHVEVHLPRDQSGEVDFQSLVSQLKETSSLQEQADILYMLYSMKGPDWNTELYEEGGATVRELLSELYVKVGEIRHWGLIRYISGILRKKVEALDEACTDLLSYQKHLTVGLPPEPREKTISAPLPYEALTKLIDEASEGDMSISTLTQEIMVYLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATEGLMNLSPSAMKNLLHHILSGKEFGVERSVRPTDSNVSPAISIHEIGAVGATKTERTGIMQLKSEIKQVEFRRLSVSMESQTSGGHPSGVDLMSPSFLSPAACIAASSGSFPTVCDHQTSKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKILQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMLADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDTMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSLCAMQ

Target ORF information:

RefSeq Version	NM_173021.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphorylase kinase alpha 1 (Phka1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173021.3

ORF Insert Sequence:

ATGAGGAGCC GCAGTAACTC GGGAGTCCGC CTGGACGGCT ATGCTCGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACA GGCTTGCTTC CAGCCAGCTA TGATCAAAAA 
GATGCCTGGG TCCGAGATAA TGTGTACAGT ATCCTGGCTG TGTGGGGTTT GGGCCTGGCA 
TATCGCAAAA ATGCTGACCG TGATGAGGAC AAGGCAAAGG CCTATGAACT GGAGCAGAGT 
GTAGTGAAGT TAATGAGGGG ACTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAG 
TCCTTCAAGT ACAGTCAGAG TACTAAAGAT AGCCTCCATG CCAAGTACAA CACCAAAACT 
TGTGCCACTG TGGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACTTCTGTG 
TACCTGCTCT TCCTAGCACA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAC 
GAAGTCAATT TTATACAGAA CCTCGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTTGGGATAT GGGAACGTGG CGATAAGACA AACCAAGGCA TCTCGGAATT GAATGCGAGT 
TCAGTTGGAA TGGCCAAGGC AGCCCTGGAA GCACTAGATG AATTAGACTT GTTTGGTGTG 
AAAGGTGGGC CACAATCAGT TATCCATGTC CTGGCTGATG AAGTCCAACA CTGCCAGTCT 
ATCCTGAATT CACTACTGCC CAGGGCTTCA ACATCCAAAG AAGTTGATGC CAGTCTGCTC 
TCAGTGGTCT CTTTCCCAGC CTTTGCTGTA GAGGACAGCC ATTTGGTGGA GCTCACCAAA 
CAGGAGATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGTTTTCT GCGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGC CTATACTATG AACCAGCTGA GCTGAAGCTA 
TTTGAAAACA TTGAGTGCGA ATGGCCATTG TTCTGGACAT ACTTTATCCT TGATGGGATC 
TTCAGTGGCA ACGTAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGATGC AGTCCTCATC 
AAGGGCAAAA ATGGAGTCCC TCTTCTTCCA GAGCTGTACA GTGTCCCTCC TGACAGGGTT 
GATGAAGAGT ATCAAAATCC CCACACTGTG GATCGAGTCC CTATGGGAAA ATTGCCTCAC 
ATGTGGGGTC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTGAA TCGTAGGTTT TCTACTGTGC CAAAGCCAGA TGTGGTGGTT 
CAAGTCTCCA TTCTGGCTGA AACAGAAGAA ATCAAGGCCA TTTTGAAGGA CAAAGGAATT 
GATGTGGAGA CCATTGCTGA AGTGTACCCC ATAAGAGTAC AGCCAGCTCG TATTCTCAGC 
CATATTTATT CTAGTCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG 
CTCATGGGTG TGCTTGGAAC ATCAAAACTT TATGACATTC GCAAAACTAT CTTTACTTTC 
ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACCA GATGATAGTA 
GAAATGCTCA GAACAGACCT TTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC 
ACGATCACTT TCCCTATCTC GCACACCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCTTGG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGGGC CAGGATCCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGCTGCA CACACTTAAG CTTCATGGAC 
CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTATGATG AAGACTATGA AGATGATTTG 
GACTCTGGCA ACTGGATGGA CAGCTATGAT TCAACAAGTA ATGATGTTCA CATGTATCTT 
CCTACAAAGT TATTTCAGCC TTCTCGTCCT TCACTCAACT TACTTGACTC CCCTGAGTCT 
CCACAAGATA GCCAGGTTCC TTCCGTTCAT GTAGAAGTGC ATCTTCCTAG GGACCAGTCT 
GGGGAAGTGG ACTTCCAGTC ATTGGTTTCA CAGTTGAAGG AGACCTCAAG CTTACAGGAG 
CAAGCTGATA TACTCTACAT GCTGTATTCA ATGAAAGGAC CTGACTGGAA CACTGAATTG 
TATGAAGAAG GGGGGGCTAC TGTCAGAGAG CTTCTTAGTG AACTGTATGT CAAAGTTGGT 
GAAATTCGGC ACTGGGGTCT GATCCGATAT ATCTCTGGGA TCTTACGGAA GAAAGTGGAG 
GCACTTGATG AGGCCTGCAC AGACCTTCTG TCCTACCAGA AACACCTGAC AGTAGGACTT 
CCTCCAGAAC CTCGAGAGAA GACCATCTCT GCGCCTCTAC CGTACGAGGC ACTCACTAAG 
CTGATAGATG AAGCCAGTGA AGGCGACATG AGCATCTCAA CCCTTACACA GGAAATAATG 
GTCTATCTTG CCATGTATAT GAGAACTCAG CCTGGCCTCT TTGCAGAAAT GTTCAGACTT 
CGAATCGGTT TGATCATACA AGTTATGGCA ACAGAACTAG CACACTCTCT TCGATGTTCA 
GCTGAGGAAG CCACAGAGGG CCTGATGAAT CTCAGTCCTT CAGCCATGAA GAACCTCCTG 
CATCACATTC TCAGTGGCAA AGAGTTTGGA GTGGAACGAA GCGTTCGTCC CACTGATTCA 
AATGTGAGTC CTGCTATTTC CATCCATGAG ATTGGTGCTG TTGGAGCAAC AAAAACTGAA 
CGAACTGGAA TCATGCAGTT AAAAAGTGAG ATAAAGCAGG TGGAATTTCG TAGGCTGTCT 
GTCTCGATGG AGAGTCAGAC TAGTGGTGGT CATCCCTCGG GTGTAGATTT GATGTCGCCA 
TCCTTTCTGT CCCCTGCAGC CTGTATTGCT GCAAGTAGTG GATCCTTTCC TACGGTGTGT 
GATCATCAGA CATCTAAAGA TAGTCGTCAA GGCCAGTGGC AACGCAGGAG AAGGCTAGAT 
GGAGCACTAA ATAGAGTACC AATTGGATTT TATCAAAAAG TATGGAAAAT TTTGCAGAAA 
TGTCATGGGC TTTCTGTGGA AGGTTTTGTT CTTCCCTCTT CAACCACTAG GGAGATGACC 
CCAGGTGAGA TTAAATTCTC TGTCCATGTG GAGTCTGTCC TGAATCGTGT CCCTCAGCCA 
GAATACCGCC AACTTCTGGT TGAAGCCATC CTTGTTCTCA CCATGCTGGC AGATATTGAA 
ATTCATAGCA TTGGGAGCAT CATTGCTGTG GAGAAAATAG TTCATATTGC CAACGACTTG 
TTCCTTCAAG AACAGAAAAC CCTCGGCGCA GATGATACCA TGTTGGCAAA GGATCCTGCA 
TCTGGCATCT GTACTCTCCT GTATGACAGT GCACCCAGTG GCAGATTTGG CACCATGACC 
TACCTCTCCA AGGCAGCTGC CACCTACGTG CAGGAGTTTC TGCCACACAG CCTCTGTGCC 
ATGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phka1 ( XM_006527894.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527957.1 Mus musculus phosphorylase kinase alpha 1 (Phka1), transcript variant X1, mRNA.

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CloneID	OMu56199
Clone ID Related Accession (Same CDS sequence)	XM_006527894.3 , XM_006527894.4
Accession Version	XM_006527894.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527894.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCC GCAGTAACTC GGGAGTCCGC CTGGACGGCT ATGCTCGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACA GGCTTGCTTC CAGCCAGCTA TGATCAAAAA 
GATGCCTGGG TCCGAGATAA TGTGTACAGT ATCCTGGCTG TGTGGGGTTT GGGCCTGGCA 
TATCGCAAAA ATGCTGACCG TGATGAGGAC AAGGCAAAGG CCTATGAACT GGAGCAGAGT 
GTAGTGAAGT TAATGAGGGG ACTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAG 
TCCTTCAAGT ACAGTCAGAG TACTAAAGAT AGCCTCCATG CCAAGTACAA CACCAAAACT 
TGTGCCACTG TGGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACTTCTGTG 
TACCTGCTCT TCCTAGCACA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAC 
GAAGTCAATT TTATACAGAA CCTCGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTTGGGATAT GGGAACGTGG CGATAAGACA AACCAAGGCA TCTCGGAATT GAATGCGAGT 
TCAGTTGGAA TGGCCAAGGC AGCCCTGGAA GCACTAGATG AATTAGACTT GTTTGGTGTG 
AAAGGTGGGC CACAATCAGT TATCCATGTC CTGGCTGATG AAGTCCAACA CTGCCAGTCT 
ATCCTGAATT CACTACTGCC CAGGGCTTCA ACATCCAAAG AAGTTGATGC CAGTCTGCTC 
TCAGTGGTCT CTTTCCCAGC CTTTGCTGTA GAGGACAGCC ATTTGGTGGA GCTCACCAAA 
CAGGAGATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGTTTTCT GCGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGC CTATACTATG AACCAGCTGA GCTGAAGCTA 
TTTGAAAACA TTGAGTGCGA ATGGCCATTG TTCTGGACAT ACTTTATCCT TGATGGGATC 
TTCAGTGGCA ACGTAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGATGC AGTCCTCATC 
AAGGGCAAAA ATGGAGTCCC TCTTCTTCCA GAGCTGTACA GTGTCCCTCC TGACAGGGTT 
GATGAAGAGT ATCAAAATCC CCACACTGTG GATCGAGTCC CTATGGGAAA ATTGCCTCAC 
ATGTGGGGTC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTGAA TCGTAGGTTT TCTACTGTGC CAAAGCCAGA TGTGGTGGTT 
CAAGTCTCCA TTCTGGCTGA AACAGAAGAA ATCAAGGCCA TTTTGAAGGA CAAAGGAATT 
GATGTGGAGA CCATTGCTGA AGTGTACCCC ATAAGAGTAC AGCCAGCTCG TATTCTCAGC 
CATATTTATT CTAGTCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG 
CTCATGGGTG TGCTTGGAAC ATCAAAACTT TATGACATTC GCAAAACTAT CTTTACTTTC 
ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACCA GATGATAGTA 
GAAATGCTCA GAACAGACCT TTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC 
ACGATCACTT TCCCTATCTC GCACACCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCTTGG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGGGC CAGGATCCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGCTGCA CACACTTAAG CTTCATGGAC 
CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTATGATG AAGACTATGA AGATGATTTG 
GACTCTGGCA ACTGGATGGA CAGCTATGAT TCAACAAGTA ATGCTCGCTG TGGTGATGAA 
GTTGCCCGTT ATTTAGACCG CCTTTTGGCA CACACTGTTC CCCATCCTAA ACTAGCTCCT 
ACCTCACGGA AGGGAGGGCT AGATCGGTTC CGAGCTGCTG TGCAAACAAC TTGCGACTTA 
ATGTCCTTGG TGGCCAAGGC CAAGGAGCTG CATATACAGA ATGTTCACAT GTATCTTCCT 
ACAAAGTTAT TTCAGCCTTC TCGTCCTTCA CTCAACTTAC TTGACTCCCC TGAGTCTCCA 
CAAGATAGCC AGGTTCCTTC CGTTCATGTA GAAGTGCATC TTCCTAGGGA CCAGTCTGGG 
GAAGTGGACT TCCAGTCATT GGTTTCACAG TTGAAGGAGA CCTCAAGCTT ACAGGAGCAA 
GCTGATATAC TCTACATGCT GTATTCAATG AAAGGACCTG ACTGGAACAC TGAATTGTAT 
GAAGAAGGGG GGGCTACTGT CAGAGAGCTT CTTAGTGAAC TGTATGTCAA AGTTGGTGAA 
ATTCGGCACT GGGGTCTGAT CCGATATATC TCTGGGATCT TACGGAAGAA AGTGGAGGCA 
CTTGATGAGG CCTGCACAGA CCTTCTGTCC TACCAGAAAC ACCTGACAGT AGGACTTCCT 
CCAGAACCTC GAGAGAAGAC CATCTCTGCG CCTCTACCGT ACGAGGCACT CACTAAGCTG 
ATAGATGAAG CCAGTGAAGG CGACATGAGC ATCTCAACCC TTACACAGGA AATAATGGTC 
TATCTTGCCA TGTATATGAG AACTCAGCCT GGCCTCTTTG CAGAAATGTT CAGACTTCGA 
ATCGGTTTGA TCATACAAGT TATGGCAACA GAACTAGCAC ACTCTCTTCG ATGTTCAGCT 
GAGGAAGCCA CAGAGGGCCT GATGAATCTC AGTCCTTCAG CCATGAAGAA CCTCCTGCAT 
CACATTCTCA GTGGCAAAGA GTTTGGAGTG GAACGAAGCG TTCGTCCCAC TGATTCAAAT 
GTGAGTCCTG CTATTTCCAT CCATGAGATT GGTGCTGTTG GAGCAACAAA AACTGAACGA 
ACTGGAATCA TGCAGTTAAA AAGTGAGATA AAGCAGACTA GTGGTGGTCA TCCCTCGGGT 
GTAGATTTGA TGTCGCCATC CTTTCTGTCC CCTGCAGCCT GTATTGCTGC AAGTAGTGGA 
TCCTTTCCTA CGGTGTGTGA TCATCAGACA TCTAAAGATA GTCGTCAAGG CCAGTGGCAA 
CGCAGGAGAA GGCTAGATGG AGCACTAAAT AGAGTACCAA TTGGATTTTA TCAAAAAGTA 
TGGAAAATTT TGCAGAAATG TCATGGGCTT TCTGTGGAAG GTTTTGTTCT TCCCTCTTCA 
ACCACTAGGG AGATGACCCC AGGTGAGATT AAATTCTCTG TCCATGTGGA GTCTGTCCTG 
AATCGTGTCC CTCAGCCAGA ATACCGCCAA CTTCTGGTTG AAGCCATCCT TGTTCTCACC 
ATGCTGGCAG ATATTGAAAT TCATAGCATT GGGAGCATCA TTGCTGTGGA GAAAATAGTT 
CATATTGCCA ACGACTTGTT CCTTCAAGAA CAGAAAACCC TCGGCGCAGA TGATACCATG 
TTGGCAAAGG ATCCTGCATC TGGCATCTGT ACTCTCCTGT ATGACAGTGC ACCCAGTGGC 
AGATTTGGCA CCATGACCTA CCTCTCCAAG GCAGCTGCCA CCTACGTGCA GGAGTTTCTG 
CCACACAGCC TCTGTGCCAT GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527957.1
CDS	360..4046
Translation	MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSLLPRASTSKEVDASLLSVVSFPAFAVEDSHLVELTKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGIFSGNVEQVQEYREALDAVLIKGKNGVPLLPELYSVPPDRVDEEYQNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKAILKDKGIDVETIAEVYPIRVQPARILSHIYSSLGCNSRMKLSGRPYRLMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNQMIVEMLRTDLSYLCSRWRMTGQPTITFPISHTMLDEDGTSLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDEDYEDDLDSGNWMDSYDSTSNARCGDEVARYLDRLLAHTVPHPKLAPTSRKGGLDRFRAAVQTTCDLMSLVAKAKELHIQNVHMYLPTKLFQPSRPSLNLLDSPESPQDSQVPSVHVEVHLPRDQSGEVDFQSLVSQLKETSSLQEQADILYMLYSMKGPDWNTELYEEGGATVRELLSELYVKVGEIRHWGLIRYISGILRKKVEALDEACTDLLSYQKHLTVGLPPEPREKTISAPLPYEALTKLIDEASEGDMSISTLTQEIMVYLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATEGLMNLSPSAMKNLLHHILSGKEFGVERSVRPTDSNVSPAISIHEIGAVGATKTERTGIMQLKSEIKQTSGGHPSGVDLMSPSFLSPAACIAASSGSFPTVCDHQTSKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKILQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMLADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDTMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSLCAMQ

Target ORF information:

RefSeq Version	XM_006527894.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphorylase kinase alpha 1 (Phka1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527894.3

ORF Insert Sequence:

ATGAGGAGCC GCAGTAACTC GGGAGTCCGC CTGGACGGCT ATGCTCGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACA GGCTTGCTTC CAGCCAGCTA TGATCAAAAA 
GATGCCTGGG TCCGAGATAA TGTGTACAGT ATCCTGGCTG TGTGGGGTTT GGGCCTGGCA 
TATCGCAAAA ATGCTGACCG TGATGAGGAC AAGGCAAAGG CCTATGAACT GGAGCAGAGT 
GTAGTGAAGT TAATGAGGGG ACTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAG 
TCCTTCAAGT ACAGTCAGAG TACTAAAGAT AGCCTCCATG CCAAGTACAA CACCAAAACT 
TGTGCCACTG TGGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACTTCTGTG 
TACCTGCTCT TCCTAGCACA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAC 
GAAGTCAATT TTATACAGAA CCTCGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTTGGGATAT GGGAACGTGG CGATAAGACA AACCAAGGCA TCTCGGAATT GAATGCGAGT 
TCAGTTGGAA TGGCCAAGGC AGCCCTGGAA GCACTAGATG AATTAGACTT GTTTGGTGTG 
AAAGGTGGGC CACAATCAGT TATCCATGTC CTGGCTGATG AAGTCCAACA CTGCCAGTCT 
ATCCTGAATT CACTACTGCC CAGGGCTTCA ACATCCAAAG AAGTTGATGC CAGTCTGCTC 
TCAGTGGTCT CTTTCCCAGC CTTTGCTGTA GAGGACAGCC ATTTGGTGGA GCTCACCAAA 
CAGGAGATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGTTTTCT GCGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGC CTATACTATG AACCAGCTGA GCTGAAGCTA 
TTTGAAAACA TTGAGTGCGA ATGGCCATTG TTCTGGACAT ACTTTATCCT TGATGGGATC 
TTCAGTGGCA ACGTAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGATGC AGTCCTCATC 
AAGGGCAAAA ATGGAGTCCC TCTTCTTCCA GAGCTGTACA GTGTCCCTCC TGACAGGGTT 
GATGAAGAGT ATCAAAATCC CCACACTGTG GATCGAGTCC CTATGGGAAA ATTGCCTCAC 
ATGTGGGGTC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTGAA TCGTAGGTTT TCTACTGTGC CAAAGCCAGA TGTGGTGGTT 
CAAGTCTCCA TTCTGGCTGA AACAGAAGAA ATCAAGGCCA TTTTGAAGGA CAAAGGAATT 
GATGTGGAGA CCATTGCTGA AGTGTACCCC ATAAGAGTAC AGCCAGCTCG TATTCTCAGC 
CATATTTATT CTAGTCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG 
CTCATGGGTG TGCTTGGAAC ATCAAAACTT TATGACATTC GCAAAACTAT CTTTACTTTC 
ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACCA GATGATAGTA 
GAAATGCTCA GAACAGACCT TTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC 
ACGATCACTT TCCCTATCTC GCACACCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCTTGG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGGGC CAGGATCCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGCTGCA CACACTTAAG CTTCATGGAC 
CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTATGATG AAGACTATGA AGATGATTTG 
GACTCTGGCA ACTGGATGGA CAGCTATGAT TCAACAAGTA ATGCTCGCTG TGGTGATGAA 
GTTGCCCGTT ATTTAGACCG CCTTTTGGCA CACACTGTTC CCCATCCTAA ACTAGCTCCT 
ACCTCACGGA AGGGAGGGCT AGATCGGTTC CGAGCTGCTG TGCAAACAAC TTGCGACTTA 
ATGTCCTTGG TGGCCAAGGC CAAGGAGCTG CATATACAGA ATGTTCACAT GTATCTTCCT 
ACAAAGTTAT TTCAGCCTTC TCGTCCTTCA CTCAACTTAC TTGACTCCCC TGAGTCTCCA 
CAAGATAGCC AGGTTCCTTC CGTTCATGTA GAAGTGCATC TTCCTAGGGA CCAGTCTGGG 
GAAGTGGACT TCCAGTCATT GGTTTCACAG TTGAAGGAGA CCTCAAGCTT ACAGGAGCAA 
GCTGATATAC TCTACATGCT GTATTCAATG AAAGGACCTG ACTGGAACAC TGAATTGTAT 
GAAGAAGGGG GGGCTACTGT CAGAGAGCTT CTTAGTGAAC TGTATGTCAA AGTTGGTGAA 
ATTCGGCACT GGGGTCTGAT CCGATATATC TCTGGGATCT TACGGAAGAA AGTGGAGGCA 
CTTGATGAGG CCTGCACAGA CCTTCTGTCC TACCAGAAAC ACCTGACAGT AGGACTTCCT 
CCAGAACCTC GAGAGAAGAC CATCTCTGCG CCTCTACCGT ACGAGGCACT CACTAAGCTG 
ATAGATGAAG CCAGTGAAGG CGACATGAGC ATCTCAACCC TTACACAGGA AATAATGGTC 
TATCTTGCCA TGTATATGAG AACTCAGCCT GGCCTCTTTG CAGAAATGTT CAGACTTCGA 
ATCGGTTTGA TCATACAAGT TATGGCAACA GAACTAGCAC ACTCTCTTCG ATGTTCAGCT 
GAGGAAGCCA CAGAGGGCCT GATGAATCTC AGTCCTTCAG CCATGAAGAA CCTCCTGCAT 
CACATTCTCA GTGGCAAAGA GTTTGGAGTG GAACGAAGCG TTCGTCCCAC TGATTCAAAT 
GTGAGTCCTG CTATTTCCAT CCATGAGATT GGTGCTGTTG GAGCAACAAA AACTGAACGA 
ACTGGAATCA TGCAGTTAAA AAGTGAGATA AAGCAGACTA GTGGTGGTCA TCCCTCGGGT 
GTAGATTTGA TGTCGCCATC CTTTCTGTCC CCTGCAGCCT GTATTGCTGC AAGTAGTGGA 
TCCTTTCCTA CGGTGTGTGA TCATCAGACA TCTAAAGATA GTCGTCAAGG CCAGTGGCAA 
CGCAGGAGAA GGCTAGATGG AGCACTAAAT AGAGTACCAA TTGGATTTTA TCAAAAAGTA 
TGGAAAATTT TGCAGAAATG TCATGGGCTT TCTGTGGAAG GTTTTGTTCT TCCCTCTTCA 
ACCACTAGGG AGATGACCCC AGGTGAGATT AAATTCTCTG TCCATGTGGA GTCTGTCCTG 
AATCGTGTCC CTCAGCCAGA ATACCGCCAA CTTCTGGTTG AAGCCATCCT TGTTCTCACC 
ATGCTGGCAG ATATTGAAAT TCATAGCATT GGGAGCATCA TTGCTGTGGA GAAAATAGTT 
CATATTGCCA ACGACTTGTT CCTTCAAGAA CAGAAAACCC TCGGCGCAGA TGATACCATG 
TTGGCAAAGG ATCCTGCATC TGGCATCTGT ACTCTCCTGT ATGACAGTGC ACCCAGTGGC 
AGATTTGGCA CCATGACCTA CCTCTCCAAG GCAGCTGCCA CCTACGTGCA GGAGTTTCTG 
CCACACAGCC TCTGTGCCAT GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phka1 ( XM_006527895.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006527958.1 Mus musculus phosphorylase kinase alpha 1 (Phka1), transcript variant X2, mRNA.

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CloneID	OMu56200
Clone ID Related Accession (Same CDS sequence)	XM_006527895.3 , XM_006527895.4
Accession Version	XM_006527895.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000086.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006527895.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCC GCAGTAACTC GGGAGTCCGC CTGGACGGCT ATGCTCGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACA GGCTTGCTTC CAGCCAGCTA TGATCAAAAA 
GATGCCTGGG TCCGAGATAA TGTGTACAGT ATCCTGGCTG TGTGGGGTTT GGGCCTGGCA 
TATCGCAAAA ATGCTGACCG TGATGAGGAC AAGGCAAAGG CCTATGAACT GGAGCAGAGT 
GTAGTGAAGT TAATGAGGGG ACTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAG 
TCCTTCAAGT ACAGTCAGAG TACTAAAGAT AGCCTCCATG CCAAGTACAA CACCAAAACT 
TGTGCCACTG TGGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACTTCTGTG 
TACCTGCTCT TCCTAGCACA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAC 
GAAGTCAATT TTATACAGAA CCTCGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTTGGGATAT GGGAACGTGG CGATAAGACA AACCAAGGCA TCTCGGAATT GAATGCGAGT 
TCAGTTGGAA TGGCCAAGGC AGCCCTGGAA GCACTAGATG AATTAGACTT GTTTGGTGTG 
AAAGGTGGGC CACAATCAGT TATCCATGTC CTGGCTGATG AAGTCCAACA CTGCCAGTCT 
ATCCTGAATT CACTACTGCC CAGGGCTTCA ACATCCAAAG AAGTTGATGC CAGTCTGCTC 
TCAGTGGTCT CTTTCCCAGC CTTTGCTGTA GAGGACAGCC ATTTGGTGGA GCTCACCAAA 
CAGGAGATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGTTTTCT GCGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGC CTATACTATG AACCAGCTGA GCTGAAGCTA 
TTTGAAAACA TTGAGTGCGA ATGGCCATTG TTCTGGACAT ACTTTATCCT TGATGGGATC 
TTCAGTGGCA ACGTAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGATGC AGTCCTCATC 
AAGGGCAAAA ATGGAGTCCC TCTTCTTCCA GAGCTGTACA GTGTCCCTCC TGACAGGGTT 
GATGAAGAGT ATCAAAATCC CCACACTGTG GATCGAGTCC CTATGGGAAA ATTGCCTCAC 
ATGTGGGGTC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTGAA TCGTAGGTTT TCTACTGTGC CAAAGCCAGA TGTGGTGGTT 
CAAGTCTCCA TTCTGGCTGA AACAGAAGAA ATCAAGGCCA TTTTGAAGGA CAAAGGAATT 
GATGTGGAGA CCATTGCTGA AGTGTACCCC ATAAGAGTAC AGCCAGCTCG TATTCTCAGC 
CATATTTATT CTAGTCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG 
CTCATGGGTG TGCTTGGAAC ATCAAAACTT TATGACATTC GCAAAACTAT CTTTACTTTC 
ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACCA GATGATAGTA 
GAAATGCTCA GAACAGACCT TTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC 
ACGATCACTT TCCCTATCTC GCACACCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCTTGG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGGGC CAGGATCCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGCTGCA CACACTTAAG CTTCATGGAC 
CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTATGATG AAGACTATGA AGATGATTTG 
GACTCTGGCA ACTGGATGGA CAGCTATGAT TCAACAAGTA ATGCTCGCTG TGGTGATGAA 
GTTGCCCGTT ATTTAGACCG CCTTTTGGCA CACACTGTTC CCCATCCTAA ACTAGCTCCT 
ACCTCACGGA AGGGAGGGCT AGATCGGTTC CGAGCTGCTG TGCAAACAAC TTGCGACTTA 
ATGTCCTTGG TGGCCAAGGC CAAGGAGCTG CATATACAGA ATGTTCACAT GTATCTTCCT 
ACAAAGTTAT TTCAGCCTTC TCGTCCTTCA CTCAACTTAC TTGACTCCCC TGAGTCTCCA 
CAAGATAGCC AGGTTCCTTC CGTTCATGTA GAAGTGCATC TTCCTAGGGA CCAGTCTGGG 
GAAGTGGACT TCCAGTCATT GGTTTCACAG TTGAAGGAGA CCTCAAGCTT ACAGGAGCAA 
GCTGATATAC TCTACATGCT GTATTCAATG AAGCCTCTAC CGTACGAGGC ACTCACTAAG 
CTGATAGATG AAGCCAGTGA AGGCGACATG AGCATCTCAA CCCTTACACA GGAAATAATG 
GTCTATCTTG CCATGTATAT GAGAACTCAG CCTGGCCTCT TTGCAGAAAT GTTCAGACTT 
CGAATCGGTT TGATCATACA AGTTATGGCA ACAGAACTAG CACACTCTCT TCGATGTTCA 
GCTGAGGAAG CCACAGAGGG CCTGATGAAT CTCAGTCCTT CAGCCATGAA GAACCTCCTG 
CATCACATTC TCAGTGGCAA AGAGTTTGGA GTGGAACGAA GCGTTCGTCC CACTGATTCA 
AATGTGAGTC CTGCTATTTC CATCCATGAG ATTGGTGCTG TTGGAGCAAC AAAAACTGAA 
CGAACTGGAA TCATGCAGTT AAAAAGTGAG ATAAAGCAGG TGGAATTTCG TAGGCTGTCT 
GTCTCGATGG AGAGTCAGAC TAGTGGTGGT CATCCCTCGG GTGTAGATTT GATGTCGCCA 
TCCTTTCTGT CCCCTGCAGC CTGTATTGCT GCAAGTAGTG GATCCTTTCC TACGGTGTGT 
GATCATCAGA CATCTAAAGA TAGTCGTCAA GGCCAGTGGC AACGCAGGAG AAGGCTAGAT 
GGAGCACTAA ATAGAGTACC AATTGGATTT TATCAAAAAG TATGGAAAAT TTTGCAGAAA 
TGTCATGGGC TTTCTGTGGA AGGTTTTGTT CTTCCCTCTT CAACCACTAG GGAGATGACC 
CCAGGTGAGA TTAAATTCTC TGTCCATGTG GAGTCTGTCC TGAATCGTGT CCCTCAGCCA 
GAATACCGCC AACTTCTGGT TGAAGCCATC CTTGTTCTCA CCATGCTGGC AGATATTGAA 
ATTCATAGCA TTGGGAGCAT CATTGCTGTG GAGAAAATAG TTCATATTGC CAACGACTTG 
TTCCTTCAAG AACAGAAAAC CCTCGGCGCA GATGATACCA TGTTGGCAAA GGATCCTGCA 
TCTGGCATCT GTACTCTCCT GTATGACAGT GCACCCAGTG GCAGATTTGG CACCATGACC 
TACCTCTCCA AGGCAGCTGC CACCTACGTG CAGGAGTTTC TGCCACACAG CCTCTGTGCC 
ATGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006527958.1
CDS	360..3848
Translation	MRSRSNSGVRLDGYARLVHQTILCHQNPVTGLLPASYDQKDAWVRDNVYSILAVWGLGLAYRKNADRDEDKAKAYELEQSVVKLMRGLLHCMIRQVDKVESFKYSQSTKDSLHAKYNTKTCATVVGDDQWGHLQLDATSVYLLFLAQMTASGLHIIHSLDEVNFIQNLVFYIEAAYKTADFGIWERGDKTNQGISELNASSVGMAKAALEALDELDLFGVKGGPQSVIHVLADEVQHCQSILNSLLPRASTSKEVDASLLSVVSFPAFAVEDSHLVELTKQEIITKLQGRYGCCRFLRDGYKTPKEDPNRLYYEPAELKLFENIECEWPLFWTYFILDGIFSGNVEQVQEYREALDAVLIKGKNGVPLLPELYSVPPDRVDEEYQNPHTVDRVPMGKLPHMWGQSLYILGSLMAEGFLAPGEIDPLNRRFSTVPKPDVVVQVSILAETEEIKAILKDKGIDVETIAEVYPIRVQPARILSHIYSSLGCNSRMKLSGRPYRLMGVLGTSKLYDIRKTIFTFTPQFIDQQQFYLALDNQMIVEMLRTDLSYLCSRWRMTGQPTITFPISHTMLDEDGTSLNSSILAALRKMQDGYFGGARIQTGKLSEFLTTSCCTHLSFMDPGPEGKLYSEDYDEDYEDDLDSGNWMDSYDSTSNARCGDEVARYLDRLLAHTVPHPKLAPTSRKGGLDRFRAAVQTTCDLMSLVAKAKELHIQNVHMYLPTKLFQPSRPSLNLLDSPESPQDSQVPSVHVEVHLPRDQSGEVDFQSLVSQLKETSSLQEQADILYMLYSMKPLPYEALTKLIDEASEGDMSISTLTQEIMVYLAMYMRTQPGLFAEMFRLRIGLIIQVMATELAHSLRCSAEEATEGLMNLSPSAMKNLLHHILSGKEFGVERSVRPTDSNVSPAISIHEIGAVGATKTERTGIMQLKSEIKQVEFRRLSVSMESQTSGGHPSGVDLMSPSFLSPAACIAASSGSFPTVCDHQTSKDSRQGQWQRRRRLDGALNRVPIGFYQKVWKILQKCHGLSVEGFVLPSSTTREMTPGEIKFSVHVESVLNRVPQPEYRQLLVEAILVLTMLADIEIHSIGSIIAVEKIVHIANDLFLQEQKTLGADDTMLAKDPASGICTLLYDSAPSGRFGTMTYLSKAAATYVQEFLPHSLCAMQ

Target ORF information:

RefSeq Version	XM_006527895.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus phosphorylase kinase alpha 1 (Phka1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006527895.3

ORF Insert Sequence:

ATGAGGAGCC GCAGTAACTC GGGAGTCCGC CTGGACGGCT ATGCTCGGCT GGTGCATCAG 
ACCATCCTGT GCCATCAGAA TCCAGTGACA GGCTTGCTTC CAGCCAGCTA TGATCAAAAA 
GATGCCTGGG TCCGAGATAA TGTGTACAGT ATCCTGGCTG TGTGGGGTTT GGGCCTGGCA 
TATCGCAAAA ATGCTGACCG TGATGAGGAC AAGGCAAAGG CCTATGAACT GGAGCAGAGT 
GTAGTGAAGT TAATGAGGGG ACTGCTGCAC TGCATGATCA GACAGGTGGA TAAAGTAGAG 
TCCTTCAAGT ACAGTCAGAG TACTAAAGAT AGCCTCCATG CCAAGTACAA CACCAAAACT 
TGTGCCACTG TGGTGGGTGA TGACCAGTGG GGACACCTGC AGTTGGATGC TACTTCTGTG 
TACCTGCTCT TCCTAGCACA AATGACTGCC TCAGGACTCC ATATCATCCA CAGCCTAGAC 
GAAGTCAATT TTATACAGAA CCTCGTGTTT TACATTGAAG CTGCATATAA AACTGCTGAC 
TTTGGGATAT GGGAACGTGG CGATAAGACA AACCAAGGCA TCTCGGAATT GAATGCGAGT 
TCAGTTGGAA TGGCCAAGGC AGCCCTGGAA GCACTAGATG AATTAGACTT GTTTGGTGTG 
AAAGGTGGGC CACAATCAGT TATCCATGTC CTGGCTGATG AAGTCCAACA CTGCCAGTCT 
ATCCTGAATT CACTACTGCC CAGGGCTTCA ACATCCAAAG AAGTTGATGC CAGTCTGCTC 
TCAGTGGTCT CTTTCCCAGC CTTTGCTGTA GAGGACAGCC ATTTGGTGGA GCTCACCAAA 
CAGGAGATCA TCACCAAGCT TCAGGGTCGT TATGGTTGCT GTCGTTTTCT GCGAGATGGA 
TATAAAACTC CTAAAGAGGA TCCCAATCGC CTATACTATG AACCAGCTGA GCTGAAGCTA 
TTTGAAAACA TTGAGTGCGA ATGGCCATTG TTCTGGACAT ACTTTATCCT TGATGGGATC 
TTCAGTGGCA ACGTAGAACA GGTTCAAGAA TATAGAGAGG CTCTTGATGC AGTCCTCATC 
AAGGGCAAAA ATGGAGTCCC TCTTCTTCCA GAGCTGTACA GTGTCCCTCC TGACAGGGTT 
GATGAAGAGT ATCAAAATCC CCACACTGTG GATCGAGTCC CTATGGGAAA ATTGCCTCAC 
ATGTGGGGTC AGTCTCTATA CATTTTAGGA AGCTTGATGG CAGAGGGATT TTTAGCTCCT 
GGAGAAATTG ATCCCCTGAA TCGTAGGTTT TCTACTGTGC CAAAGCCAGA TGTGGTGGTT 
CAAGTCTCCA TTCTGGCTGA AACAGAAGAA ATCAAGGCCA TTTTGAAGGA CAAAGGAATT 
GATGTGGAGA CCATTGCTGA AGTGTACCCC ATAAGAGTAC AGCCAGCTCG TATTCTCAGC 
CATATTTATT CTAGTCTAGG ATGCAACAGT AGAATGAAAC TCAGTGGACG ACCCTACAGG 
CTCATGGGTG TGCTTGGAAC ATCAAAACTT TATGACATTC GCAAAACTAT CTTTACTTTC 
ACTCCACAGT TTATAGACCA GCAACAGTTC TACCTGGCTC TGGACAACCA GATGATAGTA 
GAAATGCTCA GAACAGACCT TTCCTACCTC TGTAGCCGCT GGAGGATGAC AGGCCAGCCC 
ACGATCACTT TCCCTATCTC GCACACCATG CTTGATGAAG ATGGAACCAG CTTGAATTCA 
AGTATCTTGG CAGCACTCCG AAAAATGCAG GATGGCTATT TTGGTGGGGC CAGGATCCAA 
ACAGGTAAAT TGTCAGAGTT TTTGACAACA TCTTGCTGCA CACACTTAAG CTTCATGGAC 
CCTGGACCTG AGGGTAAGCT GTACAGTGAA GATTATGATG AAGACTATGA AGATGATTTG 
GACTCTGGCA ACTGGATGGA CAGCTATGAT TCAACAAGTA ATGCTCGCTG TGGTGATGAA 
GTTGCCCGTT ATTTAGACCG CCTTTTGGCA CACACTGTTC CCCATCCTAA ACTAGCTCCT 
ACCTCACGGA AGGGAGGGCT AGATCGGTTC CGAGCTGCTG TGCAAACAAC TTGCGACTTA 
ATGTCCTTGG TGGCCAAGGC CAAGGAGCTG CATATACAGA ATGTTCACAT GTATCTTCCT 
ACAAAGTTAT TTCAGCCTTC TCGTCCTTCA CTCAACTTAC TTGACTCCCC TGAGTCTCCA 
CAAGATAGCC AGGTTCCTTC CGTTCATGTA GAAGTGCATC TTCCTAGGGA CCAGTCTGGG 
GAAGTGGACT TCCAGTCATT GGTTTCACAG TTGAAGGAGA CCTCAAGCTT ACAGGAGCAA 
GCTGATATAC TCTACATGCT GTATTCAATG AAGCCTCTAC CGTACGAGGC ACTCACTAAG 
CTGATAGATG AAGCCAGTGA AGGCGACATG AGCATCTCAA CCCTTACACA GGAAATAATG 
GTCTATCTTG CCATGTATAT GAGAACTCAG CCTGGCCTCT TTGCAGAAAT GTTCAGACTT 
CGAATCGGTT TGATCATACA AGTTATGGCA ACAGAACTAG CACACTCTCT TCGATGTTCA 
GCTGAGGAAG CCACAGAGGG CCTGATGAAT CTCAGTCCTT CAGCCATGAA GAACCTCCTG 
CATCACATTC TCAGTGGCAA AGAGTTTGGA GTGGAACGAA GCGTTCGTCC CACTGATTCA 
AATGTGAGTC CTGCTATTTC CATCCATGAG ATTGGTGCTG TTGGAGCAAC AAAAACTGAA 
CGAACTGGAA TCATGCAGTT AAAAAGTGAG ATAAAGCAGG TGGAATTTCG TAGGCTGTCT 
GTCTCGATGG AGAGTCAGAC TAGTGGTGGT CATCCCTCGG GTGTAGATTT GATGTCGCCA 
TCCTTTCTGT CCCCTGCAGC CTGTATTGCT GCAAGTAGTG GATCCTTTCC TACGGTGTGT 
GATCATCAGA CATCTAAAGA TAGTCGTCAA GGCCAGTGGC AACGCAGGAG AAGGCTAGAT 
GGAGCACTAA ATAGAGTACC AATTGGATTT TATCAAAAAG TATGGAAAAT TTTGCAGAAA 
TGTCATGGGC TTTCTGTGGA AGGTTTTGTT CTTCCCTCTT CAACCACTAG GGAGATGACC 
CCAGGTGAGA TTAAATTCTC TGTCCATGTG GAGTCTGTCC TGAATCGTGT CCCTCAGCCA 
GAATACCGCC AACTTCTGGT TGAAGCCATC CTTGTTCTCA CCATGCTGGC AGATATTGAA 
ATTCATAGCA TTGGGAGCAT CATTGCTGTG GAGAAAATAG TTCATATTGC CAACGACTTG 
TTCCTTCAAG AACAGAAAAC CCTCGGCGCA GATGATACCA TGTTGGCAAA GGATCCTGCA 
TCTGGCATCT GTACTCTCCT GTATGACAGT GCACCCAGTG GCAGATTTGG CACCATGACC 
TACCTCTCCA AGGCAGCTGC CACCTACGTG CAGGAGTTTC TGCCACACAG CCTCTGTGCC 
ATGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Requirement for the Mitochondrial Pyruvate Carrier in Mammalian Development Revealed by a Hypomorphic Allelic Series.
Molecular and cellular biology36(15)2089-104(2016 Aug)
Bowman CE,Zhao L,Hartung T,Wolfgang MJ

A postnatal switch of CELF and MBNL proteins reprograms alternative splicing in the developing heart.
Proceedings of the National Academy of Sciences of the United States of America105(51)20333-8(2008 Dec)
Kalsotra A,Xiao X,Ward AJ,Castle JC,Johnson JM,Burge CB,Cooper TA

Cloning and localization of the murine Xpct gene: evidence for complex rearrangements during the evolution of the region around the Xist gene.
Genomics48(3)296-303(1998 Mar)
Debrand E,Heard E,Avner P

Phosphorylase kinase deficiency in mice.
FEBS letters10(5)328-332(1970 Oct)
Huijing F

The relation of phosphorylase to glycogenolysis in skeletal muscle and heart of mice.
The Journal of biological chemistry2381-11(1963 Jan)
LYON JB Jr,PORTER J

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Phka1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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