Pgk2 cDNA ORF clone, Mus musculus(house mouse)

Sperm function, protein phosphorylation, and metabolism differ in mice lacking successive sperm-specific glycolytic enzymes.
Huang Z, Danshina PV, Mohr K, Qu W, Goodson SG, O'Connell TM, O'Brien DA
Biology of reproduction97(4)586-597(2017 Oct)

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Bulusu V, Prior N, Snaebjornsson MT, Kuehne A, Sonnen KF, Kress J, Stein F, Schultz C, Sauer U, Aulehla A
Developmental cell40(4)331-341.e4(2017 02)

Sequence-specific promoter elements regulate temporal-specific changes in chromatin required for testis-specific activation of the Pgk2 gene.
Yang Z, Yoshioka H, McCarrey JR
Reproduction (Cambridge, England)146(5)501-16(2013)

A systematic analysis of a deep mouse epididymal sperm proteome.
Chauvin T, Xie F, Liu T, Nicora CD, Yang F, Camp DG 2nd, Smith RD, Roberts KP
Biology of reproduction87(6)141(2012 Jun)

Data from studies in transgenic mice suggest that Pgk2 core promoter elements are required to direct demethylation of DNA and reconfiguration of nucleosomes in testis during sexual maturation and spermatogenesis.
Title: Sequence-specific promoter elements regulate temporal-specific changes in chromatin required for testis-specific activation of the Pgk2 gene.

not required for the completion of spermatogenesis, but essential for sperm motility and male fertility
Title: Phosphoglycerate kinase 2 (PGK2) is essential for sperm function and male fertility in mice.

t-KH-type splicing regulatory protein binds directly to a 93-nt sequence (designated the F1 region) of the 3'-UTR of the Pgk2 mRNA and destabilizes Pgk2 mRNA constructs in testis extracts.
Title: A cytoplasmic variant of the KH-type splicing regulatory protein serves as a decay-promoting factor for phosphoglycerate kinase 2 mRNA in murine male germ cells.

Pgk2 crystal structures have been solved; the apoenzyme is in the "open" form; that is, the N-terminal domain that can bind 3-phosphoglycerate and the C-terminal domain that binds ATP are too far apart for the substrates to interact.
Title: X-ray analysis of phosphoglycerate kinase 2, a sperm-specific isoform from Mus musculus.

Data suggest that binding of PBX4, plus its coactivator PREP1, is a rate-limiting step leading to the initiation of tissue-specific transcription of the Pgk2 gene.
Title: In vivo analysis of developmentally and evolutionarily dynamic protein-DNA interactions regulating transcription of the Pgk2 gene during mammalian spermatogenesis.