PGK2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PGK2
Entrez Gene ID	5232
Full Name	phosphoglycerate kinase 2
Synonyms	HEL-S-272,PGKB,PGKPS,dJ417L20.2
General protein information	Preferred Names phosphoglycerate kinase 2 Names phosphoglycerate kinase 2 epididymis secretory protein Li 272 phosphoglycerate kinase autosomal pseudogene phosphoglycerate kinase, testis specific testicular tissue protein Li 139
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p12.3
Summary	This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.[provided by RefSeq, May 2010].
Disorder MIM:	172270

mRNA and Protein(s)

mRNA	Protein	Name
NM_138733.5	NP_620061.2	phosphoglycerate kinase 2
NM_138733.4	NP_620061.2	phosphoglycerate kinase 2

Pathways from BioSystems

Homologies

Arabidopsis thaliana (thale cress)	PGK	NP_178073.1
Oryza sativa (rice)	Os02g0169300	NP_001046020.1
Homo sapiens (human)	PGK2	NP_620061.2
	PGK2	XP_001105356.1
Canis lupus familiaris (dog)	PGK2	XP_532167.1
Mus musculus (house mouse)	Pgk2	NP_112467.2
Pan troglodytes (chimpanzee)	PGK2	XP_518531.1
Bos taurus (cattle)	PGK2	NP_001116507.1
Rattus norvegicus (Norway rat)	Pgk2	NP_001012130.1
Oryza sativa (rice)	Os06g0668200	NP_001058317.1

Gene Ontology
Bibliography

Related articles in PubMed

Sequence-specific promoter elements regulate temporal-specific changes in chromatin required for testis-specific activation of the Pgk2 gene.
Yang Z, Yoshioka H, McCarrey JR
Reproduction (Cambridge, England)146(5)501-16(2013)

Image-based genome-wide siRNA screen identifies selective autophagy factors.
Orvedahl A, Sumpter R Jr, Xiao G, Ng A, Zou Z, Tang Y, Narimatsu M, Gilpin C, Sun Q, Roth M, Forst CV, Wrana JL, Zhang YE, Luby-Phelps K, Xavier RJ, Xie Y, Levine B
Nature480(7375)113-7(2011 Dec)

Proteomic characterization of the human sperm nucleus.
de Mateo S, Castillo J, Estanyol JM, Ballesc? JL, Oliva R
Proteomics11(13)2714-26(2011 Jul)

Frequent and recent retrotransposition of orthologous genes plays a role in the evolution of sperm glycolytic enzymes.
Vemuganti SA, de Villena FP, O'Brien DA
BMC genomics11285(2010 May)

Analysis of the cDNA and encoded protein of the human testis-specific PGK-2 gene.
McCarrey JR, Kumari M, Aivaliotis MJ, Wang Z, Zhang P, Marshall F, Vandeberg JL
Developmental genetics19(4)321-32(1996)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data from studies in transgenic mice suggest that Pgk2 core promoter elements are required to direct demethylation of DNA and reconfiguration of nucleosomes in testis during sexual maturation and spermatogenesis.
Title: Sequence-specific promoter elements regulate temporal-specific changes in chromatin required for testis-specific activation of the Pgk2 gene.

The following PGK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PGK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23738	NM_138733.5 Latest version!	Homo sapiens phosphoglycerate kinase 2 (PGK2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
View Old Accession Versions >>
OHu23738	NM_138733.4	Homo sapiens phosphoglycerate kinase 2 (PGK2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PGK2 ( NM_138733.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_620061.2 Homo sapiens phosphoglycerate kinase 2 (PGK2), mRNA.

Price & Availability↑

CloneID	OHu23738
Clone ID Related Accession (Same CDS sequence)	NM_138733.5 , NM_138733.4
Accession Version	NM_138733.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	phosphoglycerate kinase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC038843.1. On May 17, 2019 this sequence version replaced NM_138733.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: SRR5189667.135441.1, SRR5189667.139664.1 [ECO:0000345] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000304801.6/ ENSP00000305995.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTCTTT CTAAGAAGTT GACTTTAGAC AAACTGGATG TTAGAGGGAA GCGAGTCATC 
ATGAGAGTAG ACTTCAATGT TCCCATGAAG AAGAACCAGA TTACAAACAA CCAGAGGATC 
AAGGCTTCCA TCCCAAGCAT CAAGTACTGC CTGGACAATG GAGCCAAGGC AGTAGTTCTT 
ATGAGTCATC TAGGTCGGCC TGATGGTGTT CCCATGCCTG ACAAATATTC CTTAGCACCT 
GTTGCTGTTG AGCTCAAATC CTTGCTGGGC AAGGATGTTC TGTTCCTGAA GGACTGTGTA 
GGCGCAGAAG TGGAGAAAGC CTGTGCCAAC CCAGCTCCTG GTTCAGTCAT CCTGCTGGAG 
AACCTGCGCT TTCATGTGGA GGAAGAAGGG AAGGGCCAAG ATCCCTCTGG AAAGAAGATT 
AAAGCTGAGC CAGATAAAAT AGAAGCCTTC CGAGCATCAC TTTCCAAGCT AGGGGACGTC 
TATGTCAATG ATGCTTTTGG CACTGCACAC CGCGCTCATA GTTCCATGGT GGGAGTGAAT 
CTGCCCCATA AAGCATCCGG ATTCTTGATG AAGAAGGAAC TAGATTACTT TGCTAAAGCC 
TTGGAAAACC CAGTGAGACC CTTTCTGGCT ATACTTGGTG GAGCCAAAGT GGCAGACAAG 
ATCCAACTTA TCAAAAATAT GCTGGACAAA GTCAATGAGA TGATTATTGG TGGTGGAATG 
GCTTATACCT TCCTTAAGGT ACTCAACAAC ATGGAGATTG GTGCTTCCCT GTTTGATGAA 
GAGGGAGCCA AGATCGTTAA AGATATCATG GCCAAAGCAC AAAAGAATGG TGTAAGGATT 
ACTTTTCCTG TTGATTTTGT TACTGGGGAC AAGTTTGACG AGAACGCTCA GGTTGGAAAA 
GCCACTGTAG CATCTGGCAT ATCTCCTGGC TGGATGGGTT TGGACTGTGG TCCTGAGAGC 
AACAAGAATC ATGCTCAAGT TGTGGCTCAA GCAAGGCTAA TTGTTTGGAA TGGGCCGTTA 
GGAGTATTTG AATGGGATGC CTTTGCTAAG GGAACCAAAG CCCTCATGGA TGAAATTGTG 
AAAGCCACTT CCAAGGGCTG CATCACTGTT ATAGGGGGTG GAGACACTGC TACTTGCTGT 
GCCAAATGGA ACACTGAAGA TAAAGTCAGC CATGTCAGCA CTGGAGGCGG TGCCAGTCTA 
GAGCTTCTGG AAGGTAAAAT CCTTCCTGGA GTAGAGGCCC TCAGCAACAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620061.2
CDS	99..1352
Translation	MSLSKKLTLDKLDVRGKRVIMRVDFNVPMKKNQITNNQRIKASIPSIKYCLDNGAKAVVLMSHLGRPDGVPMPDKYSLAPVAVELKSLLGKDVLFLKDCVGAEVEKACANPAPGSVILLENLRFHVEEEGKGQDPSGKKIKAEPDKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPHKASGFLMKKELDYFAKALENPVRPFLAILGGAKVADKIQLIKNMLDKVNEMIIGGGMAYTFLKVLNNMEIGASLFDEEGAKIVKDIMAKAQKNGVRITFPVDFVTGDKFDENAQVGKATVASGISPGWMGLDCGPESNKNHAQVVAQARLIVWNGPLGVFEWDAFAKGTKALMDEIVKATSKGCITVIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKILPGVEALSNM

Target ORF information:

RefSeq Version	NM_138733.5
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoglycerate kinase 2 (PGK2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138733.5

ORF Insert Sequence:

ATGTCTCTTT CTAAGAAGTT GACTTTAGAC AAACTGGATG TTAGAGGGAA GCGAGTCATC 
ATGAGAGTAG ACTTCAATGT TCCCATGAAG AAGAACCAGA TTACAAACAA CCAGAGGATC 
AAGGCTTCCA TCCCAAGCAT CAAGTACTGC CTGGACAATG GAGCCAAGGC AGTAGTTCTT 
ATGAGTCATC TAGGTCGGCC TGATGGTGTT CCCATGCCTG ACAAATATTC CTTAGCACCT 
GTTGCTGTTG AGCTCAAATC CTTGCTGGGC AAGGATGTTC TGTTCCTGAA GGACTGTGTA 
GGCGCAGAAG TGGAGAAAGC CTGTGCCAAC CCAGCTCCTG GTTCAGTCAT CCTGCTGGAG 
AACCTGCGCT TTCATGTGGA GGAAGAAGGG AAGGGCCAAG ATCCCTCTGG AAAGAAGATT 
AAAGCTGAGC CAGATAAAAT AGAAGCCTTC CGAGCATCAC TTTCCAAGCT AGGGGACGTC 
TATGTCAATG ATGCTTTTGG CACTGCACAC CGCGCTCATA GTTCCATGGT GGGAGTGAAT 
CTGCCCCATA AAGCATCCGG ATTCTTGATG AAGAAGGAAC TAGATTACTT TGCTAAAGCC 
TTGGAAAACC CAGTGAGACC CTTTCTGGCT ATACTTGGTG GAGCCAAAGT GGCAGACAAG 
ATCCAACTTA TCAAAAATAT GCTGGACAAA GTCAATGAGA TGATTATTGG TGGTGGAATG 
GCTTATACCT TCCTTAAGGT ACTCAACAAC ATGGAGATTG GTGCTTCCCT GTTTGATGAA 
GAGGGAGCCA AGATCGTTAA AGATATCATG GCCAAAGCAC AAAAGAATGG TGTAAGGATT 
ACTTTTCCTG TTGATTTTGT TACTGGGGAC AAGTTTGACG AGAACGCTCA GGTTGGAAAA 
GCCACTGTAG CATCTGGCAT ATCTCCTGGC TGGATGGGTT TGGACTGTGG TCCTGAGAGC 
AACAAGAATC ATGCTCAAGT TGTGGCTCAA GCAAGGCTAA TTGTTTGGAA TGGGCCGTTA 
GGAGTATTTG AATGGGATGC CTTTGCTAAG GGAACCAAAG CCCTCATGGA TGAAATTGTG 
AAAGCCACTT CCAAGGGCTG CATCACTGTT ATAGGGGGTG GAGACACTGC TACTTGCTGT 
GCCAAATGGA ACACTGAAGA TAAAGTCAGC CATGTCAGCA CTGGAGGCGG TGCCAGTCTA 
GAGCTTCTGG AAGGTAAAAT CCTTCCTGGA GTAGAGGCCC TCAGCAACAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PGK2 ( NM_138733.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_620061.2 Homo sapiens phosphoglycerate kinase 2 (PGK2), mRNA.

Price & Availability↑

CloneID	OHu23738
Clone ID Related Accession (Same CDS sequence)	NM_138733.5 , NM_138733.4
Accession Version	NM_138733.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	phosphoglycerate kinase 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB043284.1 and BC038843.1. On May 26, 2010 this sequence version replaced NM_138733.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: complete on the 3' end.

ATGTCTCTTT CTAAGAAGTT GACTTTAGAC AAACTGGATG TTAGAGGGAA GCGAGTCATC 
ATGAGAGTAG ACTTCAATGT TCCCATGAAG AAGAACCAGA TTACAAACAA CCAGAGGATC 
AAGGCTTCCA TCCCAAGCAT CAAGTACTGC CTGGACAATG GAGCCAAGGC AGTAGTTCTT 
ATGAGTCATC TAGGTCGGCC TGATGGTGTT CCCATGCCTG ACAAATATTC CTTAGCACCT 
GTTGCTGTTG AGCTCAAATC CTTGCTGGGC AAGGATGTTC TGTTCCTGAA GGACTGTGTA 
GGCGCAGAAG TGGAGAAAGC CTGTGCCAAC CCAGCTCCTG GTTCAGTCAT CCTGCTGGAG 
AACCTGCGCT TTCATGTGGA GGAAGAAGGG AAGGGCCAAG ATCCCTCTGG AAAGAAGATT 
AAAGCTGAGC CAGATAAAAT AGAAGCCTTC CGAGCATCAC TTTCCAAGCT AGGGGACGTC 
TATGTCAATG ATGCTTTTGG CACTGCACAC CGCGCTCATA GTTCCATGGT GGGAGTGAAT 
CTGCCCCATA AAGCATCCGG ATTCTTGATG AAGAAGGAAC TAGATTACTT TGCTAAAGCC 
TTGGAAAACC CAGTGAGACC CTTTCTGGCT ATACTTGGTG GAGCCAAAGT GGCAGACAAG 
ATCCAACTTA TCAAAAATAT GCTGGACAAA GTCAATGAGA TGATTATTGG TGGTGGAATG 
GCTTATACCT TCCTTAAGGT ACTCAACAAC ATGGAGATTG GTGCTTCCCT GTTTGATGAA 
GAGGGAGCCA AGATCGTTAA AGATATCATG GCCAAAGCAC AAAAGAATGG TGTAAGGATT 
ACTTTTCCTG TTGATTTTGT TACTGGGGAC AAGTTTGACG AGAACGCTCA GGTTGGAAAA 
GCCACTGTAG CATCTGGCAT ATCTCCTGGC TGGATGGGTT TGGACTGTGG TCCTGAGAGC 
AACAAGAATC ATGCTCAAGT TGTGGCTCAA GCAAGGCTAA TTGTTTGGAA TGGGCCGTTA 
GGAGTATTTG AATGGGATGC CTTTGCTAAG GGAACCAAAG CCCTCATGGA TGAAATTGTG 
AAAGCCACTT CCAAGGGCTG CATCACTGTT ATAGGGGGTG GAGACACTGC TACTTGCTGT 
GCCAAATGGA ACACTGAAGA TAAAGTCAGC CATGTCAGCA CTGGAGGCGG TGCCAGTCTA 
GAGCTTCTGG AAGGTAAAAT CCTTCCTGGA GTAGAGGCCC TCAGCAACAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_620061.2
CDS	154..1407
Translation	MSLSKKLTLDKLDVRGKRVIMRVDFNVPMKKNQITNNQRIKASIPSIKYCLDNGAKAVVLMSHLGRPDGVPMPDKYSLAPVAVELKSLLGKDVLFLKDCVGAEVEKACANPAPGSVILLENLRFHVEEEGKGQDPSGKKIKAEPDKIEAFRASLSKLGDVYVNDAFGTAHRAHSSMVGVNLPHKASGFLMKKELDYFAKALENPVRPFLAILGGAKVADKIQLIKNMLDKVNEMIIGGGMAYTFLKVLNNMEIGASLFDEEGAKIVKDIMAKAQKNGVRITFPVDFVTGDKFDENAQVGKATVASGISPGWMGLDCGPESNKNHAQVVAQARLIVWNGPLGVFEWDAFAKGTKALMDEIVKATSKGCITVIGGGDTATCCAKWNTEDKVSHVSTGGGASLELLEGKILPGVEALSNM

Target ORF information:

RefSeq Version	NM_138733.4
Organism	Homo sapiens(human)
Definition	Homo sapiens phosphoglycerate kinase 2 (PGK2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138733.4

ORF Insert Sequence:

ATGTCTCTTT CTAAGAAGTT GACTTTAGAC AAACTGGATG TTAGAGGGAA GCGAGTCATC 
ATGAGAGTAG ACTTCAATGT TCCCATGAAG AAGAACCAGA TTACAAACAA CCAGAGGATC 
AAGGCTTCCA TCCCAAGCAT CAAGTACTGC CTGGACAATG GAGCCAAGGC AGTAGTTCTT 
ATGAGTCATC TAGGTCGGCC TGATGGTGTT CCCATGCCTG ACAAATATTC CTTAGCACCT 
GTTGCTGTTG AGCTCAAATC CTTGCTGGGC AAGGATGTTC TGTTCCTGAA GGACTGTGTA 
GGCGCAGAAG TGGAGAAAGC CTGTGCCAAC CCAGCTCCTG GTTCAGTCAT CCTGCTGGAG 
AACCTGCGCT TTCATGTGGA GGAAGAAGGG AAGGGCCAAG ATCCCTCTGG AAAGAAGATT 
AAAGCTGAGC CAGATAAAAT AGAAGCCTTC CGAGCATCAC TTTCCAAGCT AGGGGACGTC 
TATGTCAATG ATGCTTTTGG CACTGCACAC CGCGCTCATA GTTCCATGGT GGGAGTGAAT 
CTGCCCCATA AAGCATCCGG ATTCTTGATG AAGAAGGAAC TAGATTACTT TGCTAAAGCC 
TTGGAAAACC CAGTGAGACC CTTTCTGGCT ATACTTGGTG GAGCCAAAGT GGCAGACAAG 
ATCCAACTTA TCAAAAATAT GCTGGACAAA GTCAATGAGA TGATTATTGG TGGTGGAATG 
GCTTATACCT TCCTTAAGGT ACTCAACAAC ATGGAGATTG GTGCTTCCCT GTTTGATGAA 
GAGGGAGCCA AGATCGTTAA AGATATCATG GCCAAAGCAC AAAAGAATGG TGTAAGGATT 
ACTTTTCCTG TTGATTTTGT TACTGGGGAC AAGTTTGACG AGAACGCTCA GGTTGGAAAA 
GCCACTGTAG CATCTGGCAT ATCTCCTGGC TGGATGGGTT TGGACTGTGG TCCTGAGAGC 
AACAAGAATC ATGCTCAAGT TGTGGCTCAA GCAAGGCTAA TTGTTTGGAA TGGGCCGTTA 
GGAGTATTTG AATGGGATGC CTTTGCTAAG GGAACCAAAG CCCTCATGGA TGAAATTGTG 
AAAGCCACTT CCAAGGGCTG CATCACTGTT ATAGGGGGTG GAGACACTGC TACTTGCTGT 
GCCAAATGGA ACACTGAAGA TAAAGTCAGC CATGTCAGCA CTGGAGGCGG TGCCAGTCTA 
GAGCTTCTGG AAGGTAAAAT CCTTCCTGGA GTAGAGGCCC TCAGCAACAT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequence-specific promoter elements regulate temporal-specific changes in chromatin required for testis-specific activation of the Pgk2 gene.
Reproduction (Cambridge, England)146(5)501-16(2013)
Yang Z,Yoshioka H,McCarrey JR

Image-based genome-wide siRNA screen identifies selective autophagy factors.
Nature480(7375)113-7(2011 Dec)
Orvedahl A,Sumpter R Jr,Xiao G,Ng A,Zou Z,Tang Y,Narimatsu M,Gilpin C,Sun Q,Roth M,Forst CV,Wrana JL,Zhang YE,Luby-Phelps K,Xavier RJ,Xie Y,Levine B

Proteomic characterization of the human sperm nucleus.
Proteomics11(13)2714-26(2011 Jul)
de Mateo S,Castillo J,Estanyol JM,Ballesc? JL,Oliva R

Frequent and recent retrotransposition of orthologous genes plays a role in the evolution of sperm glycolytic enzymes.
BMC genomics11285(2010 May)
Vemuganti SA,de Villena FP,O'Brien DA

Analysis of the cDNA and encoded protein of the human testis-specific PGK-2 gene.
Developmental genetics19(4)321-32(1996)
McCarrey JR,Kumari M,Aivaliotis MJ,Wang Z,Zhang P,Marshall F,Vandeberg JL

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PGK2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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