Enpp1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Enpp1
Entrez Gene ID	18605
Full Name	ectonucleotide pyrophosphatase/phosphodiesterase 1
Synonyms	4833416E15Rik,AI428932,C76301,CD203c,E-NPP 1,E-NPP1,Ly-41,M6S1,NPP1,Npps,PC-1,Pca,Pca-1,Pdnp1,ttw,twy
General protein information	Preferred Names ectonucleotide pyrophosphatase/phosphodiesterase 1 Names ectonucleotide pyrophosphatase/phosphodiesterase family member 1 lymphocyte antigen 41 phosphodiesterase I/nucleotide pyrophosphatase 1 plasma-cell membrane glycoprotein PC-1 tiptoe walking
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10 A4\|10 12.26 cM
Summary	This gene encodes a member of the nucleoside pyrophosphatase/phosphodiesterase family of enzymes that catalyzes the hydrolysis of pyrophosphate and phosphodiester bonds in nucleotide triphosphates and oligonucleotides, respectively, to generate nucleoside 5'-monophosphates. The encoded protein is a type II transmembrane glycoprotein that negatively regulates bone mineralization. Mice harboring a nonsense mutation in this gene, termed tiptoe walking (ttw), exhibit ectopic ossification of the spinal ligaments. The encoded protein binds to the insulin receptor, inhibits downstream signaling events and induces insulin resistance and glucose tolerance. This gene is located adjacent to a paralog on chromosome 10. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006512599.2	XP_006512662.1	ectonucleotide pyrophosphatase/phosphodiesterase family member 1 isoform X2
NM_001308327.1	NP_001295256.1	ectonucleotide pyrophosphatase/phosphodiesterase family member 1 isoform 1
NM_001308329.1	NP_001295258.1	ectonucleotide pyrophosphatase/phosphodiesterase family member 1 isoform 3
XM_006512598.4	XP_006512661.1	ectonucleotide pyrophosphatase/phosphodiesterase family member 1 isoform X1
NM_008813.4	NP_032839.3	ectonucleotide pyrophosphatase/phosphodiesterase family member 1 isoform 2
XM_006512598.3	XP_006512661.1	ectonucleotide pyrophosphatase/phosphodiesterase family member 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ENPP1	NP_006199.2
	ENPP1	XP_001103359.1
Bos taurus (cattle)	ENPP1	NP_001193141.1
Mus musculus (house mouse)	Enpp1	NP_032839.3
Rattus norvegicus (Norway rat)	Enpp1	NP_445987.1
Pan troglodytes (chimpanzee)	ENPP1	XP_518743.3
Canis lupus familiaris (dog)	ENPP1	XP_003638833.1
Caenorhabditis elegans (roundworm)	CELE_C27A7.3	NP_001041087.1
Xenopus tropicalis (tropical clawed frog)	enpp1	XP_002936360.2
Caenorhabditis elegans (roundworm)	CELE_C27A7.1	NP_001041086.1
Gallus gallus (chicken)	ENPP1	XP_424539.4
Danio rerio (zebrafish)	enpp1	NP_001025339.1
Arabidopsis thaliana (thale cress)	AT4G29680	NP_194697.1
Oryza sativa (rice)	Os01g0196600	NP_001042295.1

Gene Ontology

The following Enpp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Enpp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu68419	NM_001308329.1 Latest version!	Mus musculus ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu43767	XM_006512599.2 Latest version!	Mus musculus ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu20988	NM_008813.4 Latest version!	Mus musculus ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$531.30 $759.00
OMu68418	NM_001308327.1 Latest version!	Mus musculus ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OMu43766	XM_006512598.4 Latest version!	Mus musculus ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OMu43766	XM_006512598.3	Mus musculus ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Enpp1 ( NM_001308329.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001295258.1 Mus musculus ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), transcript variant 3, mRNA.

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CloneID	OMu68419
Clone ID Related Accession (Same CDS sequence)	NM_001308329.1
Accession Version	NM_001308329.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-28
Organism	Mus musculus(house mouse)
Product	ectonucleotide pyrophosphatase/phosphodiesterase family member 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC158616.8, AK164619.1, AC099695.15 and AI428932.1. On May 5, 2015 this sequence version replaced XM_006512600.2. Transcript Variant: This variant (3) uses alternate splice sites in two exons in the coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (3) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC050011.1, AK164619.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAGAAG AACCAGATTC TTCAGGGCAT TCACATGGAC CAGTCAGCAG CGAGGTCATC 
AAGGCCTTGC AGAAGGTTGA CCGCCTGGTC GGCATGCTGA TGGACGGCCT GAAGGACCTG 
GGCTTGGATA AATGCCTGAA CCTCATCCTC ATTTCAGATC ACGGCATGGA ACAAGGCAGC 
TGTAAGAAGT ATGTGTACCT GAATAAGTAC TTGGGGGATG TGAACAATGT GAAAGTTGTG 
TATGGACCTG CTGCTCGGTT GAGACCCACT GATGTTCCAG AGACATACTA TTCATTTAAC 
TATGAAGCCC TTGCAAAAAA TCTTTCTTGC CGGGAGCCAA ACCAGCATTT CCGGCCTTAC 
CTGAAACCCT TCTTACCCAA GCGCTTACAC TTCGCTAAAA GTGACCGGAT TGAGCCCCTG 
ACCTTCTACC TGGACCCTCA GTGGCAACTT GCGTTGAATC CATCAGAGAG GAAATATTGT 
GGAAGTGGAT TTCATGGCTC TGACAACTTG TTTTCAAACA TGCAAGCTCT TTTTATTGGC 
TATGGACCTG CCTTCAAGCA TGGTGCTGAA GTTGACTCCT TTGAAAATAT TGAAGTCTAT 
AACTTAATGT GTGACTTACT GGGTTTGATC CCAGCTCCCA ATAATGGAAG TCATGGCAGC 
CTTAACCACC TTCTAAAGAA ACCCATTTAC AACCCAAGTC ATCCCAAAGA AGAGGGCTTC 
CTGTCTCAGT GTCCAATCAA ATCAACATCC AATGACCTCG GCTGCACATG TGACCCTTGG 
ATTGTGCCAA TCAAGGACTT TGAGAAACAG CTTAATCTGA CCACAGAAGA TGATGACATT 
TACCATATGA CTGTACCCTA CGGGAGGCCC CGGATTCTAC TGAAGCAGCA CCGCGTCTGT 
CTACTCCAGC AGCAACAGTT CTTGACCGGA TACAGCCTGG ACCTCCTCAT GCCCCTCTGG 
GCGTCTTACA CCTTCCTCAG TAATGACCAA TTCTCCAGAG ATGACTTTTC CAACTGTCTG 
TACCAGGATC TTCGGATTCC CCTTAGTCCT GTGCATAAAT GTTCCTATTA CAAAAGCAAT 
TCTAAGCTAA GTTATGGGTT CCTCACGCCA CCGAGACTAA ATAGAGTTTC AAATCACATA 
TACTCTGAAG CGCTGCTTAC ATCTAATATA GTGCCAATGT ATCAGAGTTT TCAAGTTATA 
TGGCACTACC TTCATGACAC CCTACTGCAA AGGTATGCCC ATGAAAGGAA CGGCATCAAT 
GTTGTCAGCG GTCCCGTGTT TGACTTTGAT TATGACGGAC GCTATGATTC CTTAGAGATC 
CTGAAACAAA ACAGCAGAGT CATCCGAAGC CAGGAAATTC TGATTCCGAC TCACTTCTTC 
ATTGTGCTCA CCAGCTGCAA GCAGCTGTCT GAGACTCCCT TGGAGTGCTC CGCCTTAGAG 
TCCTCAGCTT ACATACTGCC TCATAGGCCT GATAACATTG AGAGCTGTAC GCATGGGAAG 
CGGGAGTCTT CATGGGTCGA GGAGTTGCTG ACCTTGCACA GAGCTCGGGT CACAGACGTG 
GAACTCATCA CTGGCCTCAG CTTCTACCAG GACCGACAAG AGTCAGTTTC AGAACTGCTG 
AGGTTGAAAA CACATTTGCC AATCTTCAGC CAAGAAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001295258.1
CDS	1199..2860
Translation	MKEEPDSSGHSHGPVSSEVIKALQKVDRLVGMLMDGLKDLGLDKCLNLILISDHGMEQGSCKKYVYLNKYLGDVNNVKVVYGPAARLRPTDVPETYYSFNYEALAKNLSCREPNQHFRPYLKPFLPKRLHFAKSDRIEPLTFYLDPQWQLALNPSERKYCGSGFHGSDNLFSNMQALFIGYGPAFKHGAEVDSFENIEVYNLMCDLLGLIPAPNNGSHGSLNHLLKKPIYNPSHPKEEGFLSQCPIKSTSNDLGCTCDPWIVPIKDFEKQLNLTTEDDDIYHMTVPYGRPRILLKQHRVCLLQQQQFLTGYSLDLLMPLWASYTFLSNDQFSRDDFSNCLYQDLRIPLSPVHKCSYYKSNSKLSYGFLTPPRLNRVSNHIYSEALLTSNIVPMYQSFQVIWHYLHDTLLQRYAHERNGINVVSGPVFDFDYDGRYDSLEILKQNSRVIRSQEILIPTHFFIVLTSCKQLSETPLECSALESSAYILPHRPDNIESCTHGKRESSWVEELLTLHRARVTDVELITGLSFYQDRQESVSELLRLKTHLPIFSQED

Target ORF information:

RefSeq Version	NM_001308329.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308329.1

ORF Insert Sequence:

ATGAAAGAAG AACCAGATTC TTCAGGGCAT TCACATGGAC CAGTCAGCAG CGAGGTCATC 
AAGGCCTTGC AGAAGGTTGA CCGCCTGGTC GGCATGCTGA TGGACGGCCT GAAGGACCTG 
GGCTTGGATA AATGCCTGAA CCTCATCCTC ATTTCAGATC ACGGCATGGA ACAAGGCAGC 
TGTAAGAAGT ATGTGTACCT GAATAAGTAC TTGGGGGATG TGAACAATGT GAAAGTTGTG 
TATGGACCTG CTGCTCGGTT GAGACCCACT GATGTTCCAG AGACATACTA TTCATTTAAC 
TATGAAGCCC TTGCAAAAAA TCTTTCTTGC CGGGAGCCAA ACCAGCATTT CCGGCCTTAC 
CTGAAACCCT TCTTACCCAA GCGCTTACAC TTCGCTAAAA GTGACCGGAT TGAGCCCCTG 
ACCTTCTACC TGGACCCTCA GTGGCAACTT GCGTTGAATC CATCAGAGAG GAAATATTGT 
GGAAGTGGAT TTCATGGCTC TGACAACTTG TTTTCAAACA TGCAAGCTCT TTTTATTGGC 
TATGGACCTG CCTTCAAGCA TGGTGCTGAA GTTGACTCCT TTGAAAATAT TGAAGTCTAT 
AACTTAATGT GTGACTTACT GGGTTTGATC CCAGCTCCCA ATAATGGAAG TCATGGCAGC 
CTTAACCACC TTCTAAAGAA ACCCATTTAC AACCCAAGTC ATCCCAAAGA AGAGGGCTTC 
CTGTCTCAGT GTCCAATCAA ATCAACATCC AATGACCTCG GCTGCACATG TGACCCTTGG 
ATTGTGCCAA TCAAGGACTT TGAGAAACAG CTTAATCTGA CCACAGAAGA TGATGACATT 
TACCATATGA CTGTACCCTA CGGGAGGCCC CGGATTCTAC TGAAGCAGCA CCGCGTCTGT 
CTACTCCAGC AGCAACAGTT CTTGACCGGA TACAGCCTGG ACCTCCTCAT GCCCCTCTGG 
GCGTCTTACA CCTTCCTCAG TAATGACCAA TTCTCCAGAG ATGACTTTTC CAACTGTCTG 
TACCAGGATC TTCGGATTCC CCTTAGTCCT GTGCATAAAT GTTCCTATTA CAAAAGCAAT 
TCTAAGCTAA GTTATGGGTT CCTCACGCCA CCGAGACTAA ATAGAGTTTC AAATCACATA 
TACTCTGAAG CGCTGCTTAC ATCTAATATA GTGCCAATGT ATCAGAGTTT TCAAGTTATA 
TGGCACTACC TTCATGACAC CCTACTGCAA AGGTATGCCC ATGAAAGGAA CGGCATCAAT 
GTTGTCAGCG GTCCCGTGTT TGACTTTGAT TATGACGGAC GCTATGATTC CTTAGAGATC 
CTGAAACAAA ACAGCAGAGT CATCCGAAGC CAGGAAATTC TGATTCCGAC TCACTTCTTC 
ATTGTGCTCA CCAGCTGCAA GCAGCTGTCT GAGACTCCCT TGGAGTGCTC CGCCTTAGAG 
TCCTCAGCTT ACATACTGCC TCATAGGCCT GATAACATTG AGAGCTGTAC GCATGGGAAG 
CGGGAGTCTT CATGGGTCGA GGAGTTGCTG ACCTTGCACA GAGCTCGGGT CACAGACGTG 
GAACTCATCA CTGGCCTCAG CTTCTACCAG GACCGACAAG AGTCAGTTTC AGAACTGCTG 
AGGTTGAAAA CACATTTGCC AATCTTCAGC CAAGAAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Enpp1 ( XM_006512599.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512662.1 Mus musculus ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu43767
Clone ID Related Accession (Same CDS sequence)	XM_006512599.2
Accession Version	XM_006512599.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ectonucleotide pyrophosphatase/phosphodiesterase family member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006512599.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGAAG AACCAGATTC TTCAGGGCAT TCACATGGAC CAGTCAGCAG CGAGGTCATC 
AAGGCCTTGC AGAAGGTTGA CCGCCTGGTC GGCATGCTGA TGGACGGCCT GAAGGACCTG 
GGCTTGGATA AATGCCTGAA CCTCATCCTC ATTTCAGATC ACGGCATGGA ACAAGGCAGC 
TGTAAGAAGT ATGTGTACCT GAATAAGTAC TTGGGGGATG TGAACAATGT GAAAGTTGTG 
TATGGACCTG CTGCTCGGTT GAGACCCACT GATGTTCCAG AGACATACTA TTCATTTAAC 
TATGAAGCCC TTGCAAAAAA TCTTTCTTGC CGGGAGCCAA ACCAGCATTT CCGGCCTTAC 
CTGAAACCCT TCTTACCCAA GCGCTTACAC TTCGCTAAAA GTGACCGGAT TGAGCCCCTG 
ACCTTCTACC TGGACCCTCA GTGGCAACTT GCGTTGAATC CATCAGAGAG GAAATATTGT 
GGAAGTGGAT TTCATGGCTC TGACAACTTG TTTTCAAACA TGCAAGCTCT TTTTATTGGC 
TATGGACCTG CCTTCAAGCA TGGTGCTGAA GTTGACTCCT TTGAAAATAT TGAAGTCTAT 
AACTTAATGT GTGACTTACT GGGTTTGATC CCAGCTCCCA ATAATGGAAG TCATGGCAGC 
CTTAACCACC TTCTAAAGAA ACCCATTTAC AACCCAAGTC ATCCCAAAGA AGAGGGCTTC 
CTGTCTCAGT GTCCAATCAA ATCAACATCC AATGACCTCG GCTGCACATG TGACCCTTGG 
ATTGTGCCAA TCAAGGACTT TGAGAAACAG CTTAATCTGA CCACAGAAGA TGTAGATGAC 
ATTTACCATA TGACTGTACC CTACGGGAGG CCCCGGATTC TACTGAAGCA GCACCGCGTC 
TGTCTACTCC AGCAGCAACA GTTCTTGACC GGATACAGCC TGGACCTCCT CATGCCCCTC 
TGGGCGTCTT ACACCTTCCT CAGTAATGAC CAATTCTCCA GAGATGACTT TTCCAACTGT 
CTGTACCAGG ATCTTCGGAT TCCCCTTAGT CCTGTGCATA AATGTTCCTA TTACAAAAGC 
AATTCTAAGC TAAGTTATGG GTTCCTCACG CCACCGAGAC TAAATAGAGT TTCAAATCAC 
ATATACTCTG AAGCGCTGCT TACATCTAAT ATAGTGCCAA TGTATCAGAG TTTTCAAGTT 
ATATGGCACT ACCTTCATGA CACCCTACTG CAAAGGTATG CCCATGAAAG GAACGGCATC 
AATGTTGTCA GCGGTCCCGT GTTTGACTTT GATTATGACG GACGCTATGA TTCCTTAGAG 
ATCCTGAAAC AAAACAGCAG AGTCATCCGA AGCCAGGAAA TTCTGATTCC GACTCACTTC 
TTCATTGTGC TCACCAGCTG CAAGCAGCTG TCTGAGACTC CCTTGGAGTG CTCCGCCTTA 
GAGTCCTCAG CTTACATACT GCCTCATAGG CCTGATAACA TTGAGAGCTG TACGCATGGG 
AAGCGGGAGT CTTCATGGGT CGAGGAGTTG CTGACCTTGC ACAGAGCTCG GGTCACAGAC 
GTGGAACTCA TCACTGGCCT CAGCTTCTAC CAGGACCGAC AAGAGTCAGT TTCAGAACTG 
CTGAGGTTGA AAACACATTT GCCAATCTTC AGCCAAGAAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512662.1
CDS	60..1724
Translation	MKEEPDSSGHSHGPVSSEVIKALQKVDRLVGMLMDGLKDLGLDKCLNLILISDHGMEQGSCKKYVYLNKYLGDVNNVKVVYGPAARLRPTDVPETYYSFNYEALAKNLSCREPNQHFRPYLKPFLPKRLHFAKSDRIEPLTFYLDPQWQLALNPSERKYCGSGFHGSDNLFSNMQALFIGYGPAFKHGAEVDSFENIEVYNLMCDLLGLIPAPNNGSHGSLNHLLKKPIYNPSHPKEEGFLSQCPIKSTSNDLGCTCDPWIVPIKDFEKQLNLTTEDVDDIYHMTVPYGRPRILLKQHRVCLLQQQQFLTGYSLDLLMPLWASYTFLSNDQFSRDDFSNCLYQDLRIPLSPVHKCSYYKSNSKLSYGFLTPPRLNRVSNHIYSEALLTSNIVPMYQSFQVIWHYLHDTLLQRYAHERNGINVVSGPVFDFDYDGRYDSLEILKQNSRVIRSQEILIPTHFFIVLTSCKQLSETPLECSALESSAYILPHRPDNIESCTHGKRESSWVEELLTLHRARVTDVELITGLSFYQDRQESVSELLRLKTHLPIFSQED

Target ORF information:

RefSeq Version	XM_006512599.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512599.2

ORF Insert Sequence:

ATGAAAGAAG AACCAGATTC TTCAGGGCAT TCACATGGAC CAGTCAGCAG CGAGGTCATC 
AAGGCCTTGC AGAAGGTTGA CCGCCTGGTC GGCATGCTGA TGGACGGCCT GAAGGACCTG 
GGCTTGGATA AATGCCTGAA CCTCATCCTC ATTTCAGATC ACGGCATGGA ACAAGGCAGC 
TGTAAGAAGT ATGTGTACCT GAATAAGTAC TTGGGGGATG TGAACAATGT GAAAGTTGTG 
TATGGACCTG CTGCTCGGTT GAGACCCACT GATGTTCCAG AGACATACTA TTCATTTAAC 
TATGAAGCCC TTGCAAAAAA TCTTTCTTGC CGGGAGCCAA ACCAGCATTT CCGGCCTTAC 
CTGAAACCCT TCTTACCCAA GCGCTTACAC TTCGCTAAAA GTGACCGGAT TGAGCCCCTG 
ACCTTCTACC TGGACCCTCA GTGGCAACTT GCGTTGAATC CATCAGAGAG GAAATATTGT 
GGAAGTGGAT TTCATGGCTC TGACAACTTG TTTTCAAACA TGCAAGCTCT TTTTATTGGC 
TATGGACCTG CCTTCAAGCA TGGTGCTGAA GTTGACTCCT TTGAAAATAT TGAAGTCTAT 
AACTTAATGT GTGACTTACT GGGTTTGATC CCAGCTCCCA ATAATGGAAG TCATGGCAGC 
CTTAACCACC TTCTAAAGAA ACCCATTTAC AACCCAAGTC ATCCCAAAGA AGAGGGCTTC 
CTGTCTCAGT GTCCAATCAA ATCAACATCC AATGACCTCG GCTGCACATG TGACCCTTGG 
ATTGTGCCAA TCAAGGACTT TGAGAAACAG CTTAATCTGA CCACAGAAGA TGTAGATGAC 
ATTTACCATA TGACTGTACC CTACGGGAGG CCCCGGATTC TACTGAAGCA GCACCGCGTC 
TGTCTACTCC AGCAGCAACA GTTCTTGACC GGATACAGCC TGGACCTCCT CATGCCCCTC 
TGGGCGTCTT ACACCTTCCT CAGTAATGAC CAATTCTCCA GAGATGACTT TTCCAACTGT 
CTGTACCAGG ATCTTCGGAT TCCCCTTAGT CCTGTGCATA AATGTTCCTA TTACAAAAGC 
AATTCTAAGC TAAGTTATGG GTTCCTCACG CCACCGAGAC TAAATAGAGT TTCAAATCAC 
ATATACTCTG AAGCGCTGCT TACATCTAAT ATAGTGCCAA TGTATCAGAG TTTTCAAGTT 
ATATGGCACT ACCTTCATGA CACCCTACTG CAAAGGTATG CCCATGAAAG GAACGGCATC 
AATGTTGTCA GCGGTCCCGT GTTTGACTTT GATTATGACG GACGCTATGA TTCCTTAGAG 
ATCCTGAAAC AAAACAGCAG AGTCATCCGA AGCCAGGAAA TTCTGATTCC GACTCACTTC 
TTCATTGTGC TCACCAGCTG CAAGCAGCTG TCTGAGACTC CCTTGGAGTG CTCCGCCTTA 
GAGTCCTCAG CTTACATACT GCCTCATAGG CCTGATAACA TTGAGAGCTG TACGCATGGG 
AAGCGGGAGT CTTCATGGGT CGAGGAGTTG CTGACCTTGC ACAGAGCTCG GGTCACAGAC 
GTGGAACTCA TCACTGGCCT CAGCTTCTAC CAGGACCGAC AAGAGTCAGT TTCAGAACTG 
CTGAGGTTGA AAACACATTT GCCAATCTTC AGCCAAGAAG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Enpp1 ( NM_008813.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032839.3 Mus musculus ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu20988
Clone ID Related Accession (Same CDS sequence)	NM_008813.4
Accession Version	NM_008813.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-28
Organism	Mus musculus(house mouse)
Product	ectonucleotide pyrophosphatase/phosphodiesterase family member 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC158616.8, AK164619.1, AK029600.1, AC099695.15 and AI428932.1. On May 5, 2015 this sequence version replaced NM_008813.3. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660813.81042.1, SRR1660819.32966.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCGCG ACGGCGACCA GGCAGGGCAC GGGCCCCGGC ACGGGTCAGC GGGAAACGGT 
CGCGAGCTCG AGTCACCAGC CGCGGCTTCG CTGCTCGCGC CCATGGACCT AGGAGAGGAG 
CCGCTGGAGA AGGCGGAGCG TGCCCGCCCT GCCAAAGACC CCAACACCTA CAAAGTGCTG 
TCGCTGGTTT TGTCAGTATG TGTGCTAACA ACAATTCTTG GTTGTATATT TGGGTTGAAA 
CCAAGCTGCG CCAAAGAAGT AAAAAGTTGC AAAGGCCGCT GCTTTGAAAG GACGTTCAGC 
AACTGTCGCT GTGATGCTGC CTGTGTCAGC CTTGGGAACT GCTGTCTGGA TTTCCAGGAG 
ACCTGTGTGG AACCAACACA TATATGGACT TGCAACAAAT TCAGGTGCGG TGAGAAGAGG 
CTGTCCAGGT TCGTGTGCTC CTGTGCAGAC GACTGCAAAA CCCACAATGA CTGTTGCATC 
AACTACAGTT CTGTGTGCCA AGATAAGAAG AGTTGGGTAG AAGAAACATG TGAAAGCATC 
GATACCCCGG AGTGTCCAGC AGAGTTTGAA TCACCCCCTA CTCTCTTGTT TTCTTTGGAT 
GGATTCAGAG CTGAGTATTT GCACACCTGG GGTGGACTTC TTCCTGTCAT TAGCAAGCTG 
AAAAACTGTG GAACATACAC TAAAAACATG AGGCCTATGT ACCCTACCAA GACGTTTCCC 
AATCATTACA GCATCGTCAC AGGACTTTAT CCGGAATCCC ATGGCATAAT TGATAACAAG 
ATGTATGATC CCAAAATGAA TGCTTCTTTC TCGCTTAAAA GTAAAGAGAA ATTCAACCCT 
TTGTGGTACA AAGGACAGCC GATTTGGGTG ACCGCTAATC ATCAGGAGGT CAAGTCCGGC 
ACATACTTTT GGCCAGGATC AGATGTGGAG ATTGACGGGA TTCTACCAGA TATCTACAAA 
GTGTATAATG GGTCAGTACC ATTTGAAGAA AGGATTTTAG CTGTTCTTGA GTGGCTGCAG 
CTTCCTAGCC ATGAAAGACC ACACTTTTAC ACTCTGTATT TAGAAGAACC AGATTCTTCA 
GGGCATTCAC ATGGACCAGT CAGCAGCGAG GTCATCAAGG CCTTGCAGAA GGTTGACCGC 
CTGGTCGGCA TGCTGATGGA CGGCCTGAAG GACCTGGGCT TGGATAAATG CCTGAACCTC 
ATCCTCATTT CAGATCACGG CATGGAACAA GGCAGCTGTA AGAAGTATGT GTACCTGAAT 
AAGTACTTGG GGGATGTGAA CAATGTGAAA GTTGTGTATG GACCTGCTGC TCGGTTGAGA 
CCCACTGATG TTCCAGAGAC ATACTATTCA TTTAACTATG AAGCCCTTGC AAAAAATCTT 
TCTTGCCGGG AGCCAAACCA GCATTTCCGG CCTTACCTGA AACCCTTCTT ACCCAAGCGC 
TTACACTTCG CTAAAAGTGA CCGGATTGAG CCCCTGACCT TCTACCTGGA CCCTCAGTGG 
CAACTTGCGT TGAATCCATC AGAGAGGAAA TATTGTGGAA GTGGATTTCA TGGCTCTGAC 
AACTTGTTTT CAAACATGCA AGCTCTTTTT ATTGGCTATG GACCTGCCTT CAAGCATGGT 
GCTGAAGTTG ACTCCTTTGA AAATATTGAA GTCTATAACT TAATGTGTGA CTTACTGGGT 
TTGATCCCAG CTCCCAATAA TGGAAGTCAT GGCAGCCTTA ACCACCTTCT AAAGAAACCC 
ATTTACAACC CAAGTCATCC CAAAGAAGAG GGCTTCCTGT CTCAGTGTCC AATCAAATCA 
ACATCCAATG ACCTCGGCTG CACATGTGAC CCTTGGATTG TGCCAATCAA GGACTTTGAG 
AAACAGCTTA ATCTGACCAC AGAAGATGAT GACATTTACC ATATGACTGT ACCCTACGGG 
AGGCCCCGGA TTCTACTGAA GCAGCACCGC GTCTGTCTAC TCCAGCAGCA ACAGTTCTTG 
ACCGGATACA GCCTGGACCT CCTCATGCCC CTCTGGGCGT CTTACACCTT CCTCAGTAAT 
GACCAATTCT CCAGAGATGA CTTTTCCAAC TGTCTGTACC AGGATCTTCG GATTCCCCTT 
AGTCCTGTGC ATAAATGTTC CTATTACAAA AGCAATTCTA AGCTAAGTTA TGGGTTCCTC 
ACGCCACCGA GACTAAATAG AGTTTCAAAT CACATATACT CTGAAGCGCT GCTTACATCT 
AATATAGTGC CAATGTATCA GAGTTTTCAA GTTATATGGC ACTACCTTCA TGACACCCTA 
CTGCAAAGGT ATGCCCATGA AAGGAACGGC ATCAATGTTG TCAGCGGTCC CGTGTTTGAC 
TTTGATTATG ACGGACGCTA TGATTCCTTA GAGATCCTGA AACAAAACAG CAGAGTCATC 
CGAAGCCAGG AAATTCTGAT TCCGACTCAC TTCTTCATTG TGCTCACCAG CTGCAAGCAG 
CTGTCTGAGA CTCCCTTGGA GTGCTCCGCC TTAGAGTCCT CAGCTTACAT ACTGCCTCAT 
AGGCCTGATA ACATTGAGAG CTGTACGCAT GGGAAGCGGG AGTCTTCATG GGTCGAGGAG 
TTGCTGACCT TGCACAGAGC TCGGGTCACA GACGTGGAAC TCATCACTGG CCTCAGCTTC 
TACCAGGACC GACAAGAGTC AGTTTCAGAA CTGCTGAGGT TGAAAACACA TTTGCCAATC 
TTCAGCCAAG AAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032839.3
CDS	169..2886
Translation	MERDGDQAGHGPRHGSAGNGRELESPAAASLLAPMDLGEEPLEKAERARPAKDPNTYKVLSLVLSVCVLTTILGCIFGLKPSCAKEVKSCKGRCFERTFSNCRCDAACVSLGNCCLDFQETCVEPTHIWTCNKFRCGEKRLSRFVCSCADDCKTHNDCCINYSSVCQDKKSWVEETCESIDTPECPAEFESPPTLLFSLDGFRAEYLHTWGGLLPVISKLKNCGTYTKNMRPMYPTKTFPNHYSIVTGLYPESHGIIDNKMYDPKMNASFSLKSKEKFNPLWYKGQPIWVTANHQEVKSGTYFWPGSDVEIDGILPDIYKVYNGSVPFEERILAVLEWLQLPSHERPHFYTLYLEEPDSSGHSHGPVSSEVIKALQKVDRLVGMLMDGLKDLGLDKCLNLILISDHGMEQGSCKKYVYLNKYLGDVNNVKVVYGPAARLRPTDVPETYYSFNYEALAKNLSCREPNQHFRPYLKPFLPKRLHFAKSDRIEPLTFYLDPQWQLALNPSERKYCGSGFHGSDNLFSNMQALFIGYGPAFKHGAEVDSFENIEVYNLMCDLLGLIPAPNNGSHGSLNHLLKKPIYNPSHPKEEGFLSQCPIKSTSNDLGCTCDPWIVPIKDFEKQLNLTTEDDDIYHMTVPYGRPRILLKQHRVCLLQQQQFLTGYSLDLLMPLWASYTFLSNDQFSRDDFSNCLYQDLRIPLSPVHKCSYYKSNSKLSYGFLTPPRLNRVSNHIYSEALLTSNIVPMYQSFQVIWHYLHDTLLQRYAHERNGINVVSGPVFDFDYDGRYDSLEILKQNSRVIRSQEILIPTHFFIVLTSCKQLSETPLECSALESSAYILPHRPDNIESCTHGKRESSWVEELLTLHRARVTDVELITGLSFYQDRQESVSELLRLKTHLPIFSQED

Target ORF information:

RefSeq Version	NM_008813.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008813.4

ORF Insert Sequence:

ATGGAGCGCG ACGGCGACCA GGCAGGGCAC GGGCCCCGGC ACGGGTCAGC GGGAAACGGT 
CGCGAGCTCG AGTCACCAGC CGCGGCTTCG CTGCTCGCGC CCATGGACCT AGGAGAGGAG 
CCGCTGGAGA AGGCGGAGCG TGCCCGCCCT GCCAAAGACC CCAACACCTA CAAAGTGCTG 
TCGCTGGTTT TGTCAGTATG TGTGCTAACA ACAATTCTTG GTTGTATATT TGGGTTGAAA 
CCAAGCTGCG CCAAAGAAGT AAAAAGTTGC AAAGGCCGCT GCTTTGAAAG GACGTTCAGC 
AACTGTCGCT GTGATGCTGC CTGTGTCAGC CTTGGGAACT GCTGTCTGGA TTTCCAGGAG 
ACCTGTGTGG AACCAACACA TATATGGACT TGCAACAAAT TCAGGTGCGG TGAGAAGAGG 
CTGTCCAGGT TCGTGTGCTC CTGTGCAGAC GACTGCAAAA CCCACAATGA CTGTTGCATC 
AACTACAGTT CTGTGTGCCA AGATAAGAAG AGTTGGGTAG AAGAAACATG TGAAAGCATC 
GATACCCCGG AGTGTCCAGC AGAGTTTGAA TCACCCCCTA CTCTCTTGTT TTCTTTGGAT 
GGATTCAGAG CTGAGTATTT GCACACCTGG GGTGGACTTC TTCCTGTCAT TAGCAAGCTG 
AAAAACTGTG GAACATACAC TAAAAACATG AGGCCTATGT ACCCTACCAA GACGTTTCCC 
AATCATTACA GCATCGTCAC AGGACTTTAT CCGGAATCCC ATGGCATAAT TGATAACAAG 
ATGTATGATC CCAAAATGAA TGCTTCTTTC TCGCTTAAAA GTAAAGAGAA ATTCAACCCT 
TTGTGGTACA AAGGACAGCC GATTTGGGTG ACCGCTAATC ATCAGGAGGT CAAGTCCGGC 
ACATACTTTT GGCCAGGATC AGATGTGGAG ATTGACGGGA TTCTACCAGA TATCTACAAA 
GTGTATAATG GGTCAGTACC ATTTGAAGAA AGGATTTTAG CTGTTCTTGA GTGGCTGCAG 
CTTCCTAGCC ATGAAAGACC ACACTTTTAC ACTCTGTATT TAGAAGAACC AGATTCTTCA 
GGGCATTCAC ATGGACCAGT CAGCAGCGAG GTCATCAAGG CCTTGCAGAA GGTTGACCGC 
CTGGTCGGCA TGCTGATGGA CGGCCTGAAG GACCTGGGCT TGGATAAATG CCTGAACCTC 
ATCCTCATTT CAGATCACGG CATGGAACAA GGCAGCTGTA AGAAGTATGT GTACCTGAAT 
AAGTACTTGG GGGATGTGAA CAATGTGAAA GTTGTGTATG GACCTGCTGC TCGGTTGAGA 
CCCACTGATG TTCCAGAGAC ATACTATTCA TTTAACTATG AAGCCCTTGC AAAAAATCTT 
TCTTGCCGGG AGCCAAACCA GCATTTCCGG CCTTACCTGA AACCCTTCTT ACCCAAGCGC 
TTACACTTCG CTAAAAGTGA CCGGATTGAG CCCCTGACCT TCTACCTGGA CCCTCAGTGG 
CAACTTGCGT TGAATCCATC AGAGAGGAAA TATTGTGGAA GTGGATTTCA TGGCTCTGAC 
AACTTGTTTT CAAACATGCA AGCTCTTTTT ATTGGCTATG GACCTGCCTT CAAGCATGGT 
GCTGAAGTTG ACTCCTTTGA AAATATTGAA GTCTATAACT TAATGTGTGA CTTACTGGGT 
TTGATCCCAG CTCCCAATAA TGGAAGTCAT GGCAGCCTTA ACCACCTTCT AAAGAAACCC 
ATTTACAACC CAAGTCATCC CAAAGAAGAG GGCTTCCTGT CTCAGTGTCC AATCAAATCA 
ACATCCAATG ACCTCGGCTG CACATGTGAC CCTTGGATTG TGCCAATCAA GGACTTTGAG 
AAACAGCTTA ATCTGACCAC AGAAGATGAT GACATTTACC ATATGACTGT ACCCTACGGG 
AGGCCCCGGA TTCTACTGAA GCAGCACCGC GTCTGTCTAC TCCAGCAGCA ACAGTTCTTG 
ACCGGATACA GCCTGGACCT CCTCATGCCC CTCTGGGCGT CTTACACCTT CCTCAGTAAT 
GACCAATTCT CCAGAGATGA CTTTTCCAAC TGTCTGTACC AGGATCTTCG GATTCCCCTT 
AGTCCTGTGC ATAAATGTTC CTATTACAAA AGCAATTCTA AGCTAAGTTA TGGGTTCCTC 
ACGCCACCGA GACTAAATAG AGTTTCAAAT CACATATACT CTGAAGCGCT GCTTACATCT 
AATATAGTGC CAATGTATCA GAGTTTTCAA GTTATATGGC ACTACCTTCA TGACACCCTA 
CTGCAAAGGT ATGCCCATGA AAGGAACGGC ATCAATGTTG TCAGCGGTCC CGTGTTTGAC 
TTTGATTATG ACGGACGCTA TGATTCCTTA GAGATCCTGA AACAAAACAG CAGAGTCATC 
CGAAGCCAGG AAATTCTGAT TCCGACTCAC TTCTTCATTG TGCTCACCAG CTGCAAGCAG 
CTGTCTGAGA CTCCCTTGGA GTGCTCCGCC TTAGAGTCCT CAGCTTACAT ACTGCCTCAT 
AGGCCTGATA ACATTGAGAG CTGTACGCAT GGGAAGCGGG AGTCTTCATG GGTCGAGGAG 
TTGCTGACCT TGCACAGAGC TCGGGTCACA GACGTGGAAC TCATCACTGG CCTCAGCTTC 
TACCAGGACC GACAAGAGTC AGTTTCAGAA CTGCTGAGGT TGAAAACACA TTTGCCAATC 
TTCAGCCAAG AAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Enpp1 ( XM_006512598.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512661.1 Mus musculus ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), transcript variant X1, mRNA.

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CloneID	OMu43766
Clone ID Related Accession (Same CDS sequence)	XM_006512598.4 , XM_006512598.3
Accession Version	XM_006512598.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	ectonucleotide pyrophosphatase/phosphodiesterase family member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512598.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAC ACAAGAGGAG AGTAGATAAC TGTGCTCTCT GCAGATCTCC CCAACAGATC 
TCTCATTTGA AGGACATGAC AAAGGTTTTG TCAGTATGTG TGCTAACAAC AATTCTTGGT 
TGTATATTTG GGTTGAAACC AAGCTGCGCC AAAGAAGTAA AAAGTTGCAA AGGCCGCTGC 
TTTGAAAGGA CGTTCAGCAA CTGTCGCTGT GATGCTGCCT GTGTCAGCCT TGGGAACTGC 
TGTCTGGATT TCCAGGAGAC CTGTGTGGAA CCAACACATA TATGGACTTG CAACAAATTC 
AGGTGCGGTG AGAAGAGGCT GTCCAGGTTC GTGTGCTCCT GTGCAGACGA CTGCAAAACC 
CACAATGACT GTTGCATCAA CTACAGTTCT GTGTGCCAAG ATAAGAAGAG TTGGGTAGAA 
GAAACATGTG AAAGCATCGA TACCCCGGAG TGTCCAGCAG AGTTTGAATC ACCCCCTACT 
CTCTTGTTTT CTTTGGATGG ATTCAGAGCT GAGTATTTGC ACACCTGGGG TGGACTTCTT 
CCTGTCATTA GCAAGCTGAA AAACTGTGGA ACATACACTA AAAACATGAG GCCTATGTAC 
CCTACCAAGA CGTTTCCCAA TCATTACAGC ATCGTCACAG GACTTTATCC GGAATCCCAT 
GGCATAATTG ATAACAAGAT GTATGATCCC AAAATGAATG CTTCTTTCTC GCTTAAAAGT 
AAAGAGAAAT TCAACCCTTT GTGGTACAAA GGACAGCCGA TTTGGGTGAC CGCTAATCAT 
CAGGAGGTCA AGTCCGGCAC ATACTTTTGG CCAGGATCAG ATGTGGAGAT TGACGGGATT 
CTACCAGATA TCTACAAAGT GTATAATGGG TCAGTACCAT TTGAAGAAAG GATTTTAGCT 
GTTCTTGAGT GGCTGCAGCT TCCTAGCCAT GAAAGACCAC ACTTTTACAC TCTGTATTTA 
GAAGAACCAG ATTCTTCAGG GCATTCACAT GGACCAGTCA GCAGCGAGGT CATCAAGGCC 
TTGCAGAAGG TTGACCGCCT GGTCGGCATG CTGATGGACG GCCTGAAGGA CCTGGGCTTG 
GATAAATGCC TGAACCTCAT CCTCATTTCA GATCACGGCA TGGAACAAGG CAGCTGTAAG 
AAGTATGTGT ACCTGAATAA GTACTTGGGG GATGTGAACA ATGTGAAAGT TGTGTATGGA 
CCTGCTGCTC GGTTGAGACC CACTGATGTT CCAGAGACAT ACTATTCATT TAACTATGAA 
GCCCTTGCAA AAAATCTTTC TTGCCGGGAG CCAAACCAGC ATTTCCGGCC TTACCTGAAA 
CCCTTCTTAC CCAAGCGCTT ACACTTCGCT AAAAGTGACC GGATTGAGCC CCTGACCTTC 
TACCTGGACC CTCAGTGGCA ACTTGCGTTG AATCCATCAG AGAGGAAATA TTGTGGAAGT 
GGATTTCATG GCTCTGACAA CTTGTTTTCA AACATGCAAG CTCTTTTTAT TGGCTATGGA 
CCTGCCTTCA AGCATGGTGC TGAAGTTGAC TCCTTTGAAA ATATTGAAGT CTATAACTTA 
ATGTGTGACT TACTGGGTTT GATCCCAGCT CCCAATAATG GAAGTCATGG CAGCCTTAAC 
CACCTTCTAA AGAAACCCAT TTACAACCCA AGTCATCCCA AAGAAGAGGG CTTCCTGTCT 
CAGTGTCCAA TCAAATCAAC ATCCAATGAC CTCGGCTGCA CATGTGACCC TTGGATTGTG 
CCAATCAAGG ACTTTGAGAA ACAGCTTAAT CTGACCACAG AAGATGTAGA TGACATTTAC 
CATATGACTG TACCCTACGG GAGGCCCCGG ATTCTACTGA AGCAGCACCG CGTCTGTCTA 
CTCCAGCAGC AACAGTTCTT GACCGGATAC AGCCTGGACC TCCTCATGCC CCTCTGGGCG 
TCTTACACCT TCCTCAGTAA TGACCAATTC TCCAGAGATG ACTTTTCCAA CTGTCTGTAC 
CAGGATCTTC GGATTCCCCT TAGTCCTGTG CATAAATGTT CCTATTACAA AAGCAATTCT 
AAGCTAAGTT ATGGGTTCCT CACGCCACCG AGACTAAATA GAGTTTCAAA TCACATATAC 
TCTGAAGCGC TGCTTACATC TAATATAGTG CCAATGTATC AGAGTTTTCA AGTTATATGG 
CACTACCTTC ATGACACCCT ACTGCAAAGG TATGCCCATG AAAGGAACGG CATCAATGTT 
GTCAGCGGTC CCGTGTTTGA CTTTGATTAT GACGGACGCT ATGATTCCTT AGAGATCCTG 
AAACAAAACA GCAGAGTCAT CCGAAGCCAG GAAATTCTGA TTCCGACTCA CTTCTTCATT 
GTGCTCACCA GCTGCAAGCA GCTGTCTGAG ACTCCCTTGG AGTGCTCCGC CTTAGAGTCC 
TCAGCTTACA TACTGCCTCA TAGGCCTGAT AACATTGAGA GCTGTACGCA TGGGAAGCGG 
GAGTCTTCAT GGGTCGAGGA GTTGCTGACC TTGCACAGAG CTCGGGTCAC AGACGTGGAA 
CTCATCACTG GCCTCAGCTT CTACCAGGAC CGACAAGAGT CAGTTTCAGA ACTGCTGAGG 
TTGAAAACAC ATTTGCCAAT CTTCAGCCAA GAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512661.1
CDS	16..2634
Translation	MIEHKRRVDNCALCRSPQQISHLKDMTKVLSVCVLTTILGCIFGLKPSCAKEVKSCKGRCFERTFSNCRCDAACVSLGNCCLDFQETCVEPTHIWTCNKFRCGEKRLSRFVCSCADDCKTHNDCCINYSSVCQDKKSWVEETCESIDTPECPAEFESPPTLLFSLDGFRAEYLHTWGGLLPVISKLKNCGTYTKNMRPMYPTKTFPNHYSIVTGLYPESHGIIDNKMYDPKMNASFSLKSKEKFNPLWYKGQPIWVTANHQEVKSGTYFWPGSDVEIDGILPDIYKVYNGSVPFEERILAVLEWLQLPSHERPHFYTLYLEEPDSSGHSHGPVSSEVIKALQKVDRLVGMLMDGLKDLGLDKCLNLILISDHGMEQGSCKKYVYLNKYLGDVNNVKVVYGPAARLRPTDVPETYYSFNYEALAKNLSCREPNQHFRPYLKPFLPKRLHFAKSDRIEPLTFYLDPQWQLALNPSERKYCGSGFHGSDNLFSNMQALFIGYGPAFKHGAEVDSFENIEVYNLMCDLLGLIPAPNNGSHGSLNHLLKKPIYNPSHPKEEGFLSQCPIKSTSNDLGCTCDPWIVPIKDFEKQLNLTTEDVDDIYHMTVPYGRPRILLKQHRVCLLQQQQFLTGYSLDLLMPLWASYTFLSNDQFSRDDFSNCLYQDLRIPLSPVHKCSYYKSNSKLSYGFLTPPRLNRVSNHIYSEALLTSNIVPMYQSFQVIWHYLHDTLLQRYAHERNGINVVSGPVFDFDYDGRYDSLEILKQNSRVIRSQEILIPTHFFIVLTSCKQLSETPLECSALESSAYILPHRPDNIESCTHGKRESSWVEELLTLHRARVTDVELITGLSFYQDRQESVSELLRLKTHLPIFSQED

Target ORF information:

RefSeq Version	XM_006512598.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512598.3

ORF Insert Sequence:

ATGATTGAAC ACAAGAGGAG AGTAGATAAC TGTGCTCTCT GCAGATCTCC CCAACAGATC 
TCTCATTTGA AGGACATGAC AAAGGTTTTG TCAGTATGTG TGCTAACAAC AATTCTTGGT 
TGTATATTTG GGTTGAAACC AAGCTGCGCC AAAGAAGTAA AAAGTTGCAA AGGCCGCTGC 
TTTGAAAGGA CGTTCAGCAA CTGTCGCTGT GATGCTGCCT GTGTCAGCCT TGGGAACTGC 
TGTCTGGATT TCCAGGAGAC CTGTGTGGAA CCAACACATA TATGGACTTG CAACAAATTC 
AGGTGCGGTG AGAAGAGGCT GTCCAGGTTC GTGTGCTCCT GTGCAGACGA CTGCAAAACC 
CACAATGACT GTTGCATCAA CTACAGTTCT GTGTGCCAAG ATAAGAAGAG TTGGGTAGAA 
GAAACATGTG AAAGCATCGA TACCCCGGAG TGTCCAGCAG AGTTTGAATC ACCCCCTACT 
CTCTTGTTTT CTTTGGATGG ATTCAGAGCT GAGTATTTGC ACACCTGGGG TGGACTTCTT 
CCTGTCATTA GCAAGCTGAA AAACTGTGGA ACATACACTA AAAACATGAG GCCTATGTAC 
CCTACCAAGA CGTTTCCCAA TCATTACAGC ATCGTCACAG GACTTTATCC GGAATCCCAT 
GGCATAATTG ATAACAAGAT GTATGATCCC AAAATGAATG CTTCTTTCTC GCTTAAAAGT 
AAAGAGAAAT TCAACCCTTT GTGGTACAAA GGACAGCCGA TTTGGGTGAC CGCTAATCAT 
CAGGAGGTCA AGTCCGGCAC ATACTTTTGG CCAGGATCAG ATGTGGAGAT TGACGGGATT 
CTACCAGATA TCTACAAAGT GTATAATGGG TCAGTACCAT TTGAAGAAAG GATTTTAGCT 
GTTCTTGAGT GGCTGCAGCT TCCTAGCCAT GAAAGACCAC ACTTTTACAC TCTGTATTTA 
GAAGAACCAG ATTCTTCAGG GCATTCACAT GGACCAGTCA GCAGCGAGGT CATCAAGGCC 
TTGCAGAAGG TTGACCGCCT GGTCGGCATG CTGATGGACG GCCTGAAGGA CCTGGGCTTG 
GATAAATGCC TGAACCTCAT CCTCATTTCA GATCACGGCA TGGAACAAGG CAGCTGTAAG 
AAGTATGTGT ACCTGAATAA GTACTTGGGG GATGTGAACA ATGTGAAAGT TGTGTATGGA 
CCTGCTGCTC GGTTGAGACC CACTGATGTT CCAGAGACAT ACTATTCATT TAACTATGAA 
GCCCTTGCAA AAAATCTTTC TTGCCGGGAG CCAAACCAGC ATTTCCGGCC TTACCTGAAA 
CCCTTCTTAC CCAAGCGCTT ACACTTCGCT AAAAGTGACC GGATTGAGCC CCTGACCTTC 
TACCTGGACC CTCAGTGGCA ACTTGCGTTG AATCCATCAG AGAGGAAATA TTGTGGAAGT 
GGATTTCATG GCTCTGACAA CTTGTTTTCA AACATGCAAG CTCTTTTTAT TGGCTATGGA 
CCTGCCTTCA AGCATGGTGC TGAAGTTGAC TCCTTTGAAA ATATTGAAGT CTATAACTTA 
ATGTGTGACT TACTGGGTTT GATCCCAGCT CCCAATAATG GAAGTCATGG CAGCCTTAAC 
CACCTTCTAA AGAAACCCAT TTACAACCCA AGTCATCCCA AAGAAGAGGG CTTCCTGTCT 
CAGTGTCCAA TCAAATCAAC ATCCAATGAC CTCGGCTGCA CATGTGACCC TTGGATTGTG 
CCAATCAAGG ACTTTGAGAA ACAGCTTAAT CTGACCACAG AAGATGTAGA TGACATTTAC 
CATATGACTG TACCCTACGG GAGGCCCCGG ATTCTACTGA AGCAGCACCG CGTCTGTCTA 
CTCCAGCAGC AACAGTTCTT GACCGGATAC AGCCTGGACC TCCTCATGCC CCTCTGGGCG 
TCTTACACCT TCCTCAGTAA TGACCAATTC TCCAGAGATG ACTTTTCCAA CTGTCTGTAC 
CAGGATCTTC GGATTCCCCT TAGTCCTGTG CATAAATGTT CCTATTACAA AAGCAATTCT 
AAGCTAAGTT ATGGGTTCCT CACGCCACCG AGACTAAATA GAGTTTCAAA TCACATATAC 
TCTGAAGCGC TGCTTACATC TAATATAGTG CCAATGTATC AGAGTTTTCA AGTTATATGG 
CACTACCTTC ATGACACCCT ACTGCAAAGG TATGCCCATG AAAGGAACGG CATCAATGTT 
GTCAGCGGTC CCGTGTTTGA CTTTGATTAT GACGGACGCT ATGATTCCTT AGAGATCCTG 
AAACAAAACA GCAGAGTCAT CCGAAGCCAG GAAATTCTGA TTCCGACTCA CTTCTTCATT 
GTGCTCACCA GCTGCAAGCA GCTGTCTGAG ACTCCCTTGG AGTGCTCCGC CTTAGAGTCC 
TCAGCTTACA TACTGCCTCA TAGGCCTGAT AACATTGAGA GCTGTACGCA TGGGAAGCGG 
GAGTCTTCAT GGGTCGAGGA GTTGCTGACC TTGCACAGAG CTCGGGTCAC AGACGTGGAA 
CTCATCACTG GCCTCAGCTT CTACCAGGAC CGACAAGAGT CAGTTTCAGA ACTGCTGAGG 
TTGAAAACAC ATTTGCCAAT CTTCAGCCAA GAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Enpp1 ( NM_001308327.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001295256.1 Mus musculus ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), transcript variant 1, mRNA.

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CloneID	OMu68418
Clone ID Related Accession (Same CDS sequence)	NM_001308327.1
Accession Version	NM_001308327.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-28
Organism	Mus musculus(house mouse)
Product	ectonucleotide pyrophosphatase/phosphodiesterase family member 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC158616.8, AF339910.1, AC099695.15 and AI428932.1. On May 5, 2015 this sequence version replaced XM_006512597.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF339910.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCGCG ACGGCGACCA GGCAGGGCAC GGGCCCCGGC ACGGGTCAGC GGGAAACGGT 
CGCGAGCTCG AGTCACCAGC CGCGGCTTCG CTGCTCGCGC CCATGGACCT AGGAGAGGAG 
CCGCTGGAGA AGGCGGAGCG TGCCCGCCCT GCCAAAGACC CCAACACCTA CAAAGTGCTG 
TCGCTGGTTT TGTCAGTATG TGTGCTAACA ACAATTCTTG GTTGTATATT TGGGTTGAAA 
CCAAGCTGCG CCAAAGAAGT AAAAAGTTGC AAAGGCCGCT GCTTTGAAAG GACGTTCAGC 
AACTGTCGCT GTGATGCTGC CTGTGTCAGC CTTGGGAACT GCTGTCTGGA TTTCCAGGAG 
ACCTGTGTGG AACCAACACA TATATGGACT TGCAACAAAT TCAGGTGCGG TGAGAAGAGG 
CTGTCCAGGT TCGTGTGCTC CTGTGCAGAC GACTGCAAAA CCCACAATGA CTGTTGCATC 
AACTACAGTT CTGTGTGCCA AGATAAGAAG AGTTGGGTAG AAGAAACATG TGAAAGCATC 
GATACCCCGG AGTGTCCAGC AGAGTTTGAA TCACCCCCTA CTCTCTTGTT TTCTTTGGAT 
GGATTCAGAG CTGAGTATTT GCACACCTGG GGTGGACTTC TTCCTGTCAT TAGCAAGCTG 
AAAAACTGTG GAACATACAC TAAAAACATG AGGCCTATGT ACCCTACCAA GACGTTTCCC 
AATCATTACA GCATCGTCAC AGGACTTTAT CCGGAATCCC ATGGCATAAT TGATAACAAG 
ATGTATGATC CCAAAATGAA TGCTTCTTTC TCGCTTAAAA GTAAAGAGAA ATTCAACCCT 
TTGTGGTACA AAGGACAGCC GATTTGGGTG ACCGCTAATC ATCAGGAGGT CAAGTCCGGC 
ACATACTTTT GGCCAGGATC AGATGTGGAG ATTGACGGGA TTCTACCAGA TATCTACAAA 
GTGTATAATG GGTCAGTACC ATTTGAAGAA AGGATTTTAG CTGTTCTTGA GTGGCTGCAG 
CTTCCTAGCC ATGAAAGACC ACACTTTTAC ACTCTGTATT TAGAAGAACC AGATTCTTCA 
GGGCATTCAC ATGGACCAGT CAGCAGCGAG GTCATCAAGG CCTTGCAGAA GGTTGACCGC 
CTGGTCGGCA TGCTGATGGA CGGCCTGAAG GACCTGGGCT TGGATAAATG CCTGAACCTC 
ATCCTCATTT CAGATCACGG CATGGAACAA GGCAGCTGTA AGAAGTATGT GTACCTGAAT 
AAGTACTTGG GGGATGTGAA CAATGTGAAA GTTGTGTATG GACCTGCTGC TCGGTTGAGA 
CCCACTGATG TTCCAGAGAC ATACTATTCA TTTAACTATG AAGCCCTTGC AAAAAATCTT 
TCTTGCCGGG AGCCAAACCA GCATTTCCGG CCTTACCTGA AACCCTTCTT ACCCAAGCGC 
TTACACTTCG CTAAAAGTGA CCGGATTGAG CCCCTGACCT TCTACCTGGA CCCTCAGTGG 
CAACTTGCGT TGAATCCATC AGAGAGGAAA TATTGTGGAA GTGGATTTCA TGGCTCTGAC 
AACTTGTTTT CAAACATGCA AGCTCTTTTT ATTGGCTATG GACCTGCCTT CAAGCATGGT 
GCTGAAGTTG ACTCCTTTGA AAATATTGAA GTCTATAACT TAATGTGTGA CTTACTGGGT 
TTGATCCCAG CTCCCAATAA TGGAAGTCAT GGCAGCCTTA ACCACCTTCT AAAGAAACCC 
ATTTACAACC CAAGTCATCC CAAAGAAGAG GGCTTCCTGT CTCAGTGTCC AATCAAATCA 
ACATCCAATG ACCTCGGCTG CACATGTGAC CCTTGGATTG TGCCAATCAA GGACTTTGAG 
AAACAGCTTA ATCTGACCAC AGAAGATGTA GATGACATTT ACCATATGAC TGTACCCTAC 
GGGAGGCCCC GGATTCTACT GAAGCAGCAC CGCGTCTGTC TACTCCAGCA GCAACAGTTC 
TTGACCGGAT ACAGCCTGGA CCTCCTCATG CCCCTCTGGG CGTCTTACAC CTTCCTCAGT 
AATGACCAAT TCTCCAGAGA TGACTTTTCC AACTGTCTGT ACCAGGATCT TCGGATTCCC 
CTTAGTCCTG TGCATAAATG TTCCTATTAC AAAAGCAATT CTAAGCTAAG TTATGGGTTC 
CTCACGCCAC CGAGACTAAA TAGAGTTTCA AATCACATAT ACTCTGAAGC GCTGCTTACA 
TCTAATATAG TGCCAATGTA TCAGAGTTTT CAAGTTATAT GGCACTACCT TCATGACACC 
CTACTGCAAA GGTATGCCCA TGAAAGGAAC GGCATCAATG TTGTCAGCGG TCCCGTGTTT 
GACTTTGATT ATGACGGACG CTATGATTCC TTAGAGATCC TGAAACAAAA CAGCAGAGTC 
ATCCGAAGCC AGGAAATTCT GATTCCGACT CACTTCTTCA TTGTGCTCAC CAGCTGCAAG 
CAGCTGTCTG AGACTCCCTT GGAGTGCTCC GCCTTAGAGT CCTCAGCTTA CATACTGCCT 
CATAGGCCTG ATAACATTGA GAGCTGTACG CATGGGAAGC GGGAGTCTTC ATGGGTCGAG 
GAGTTGCTGA CCTTGCACAG AGCTCGGGTC ACAGACGTGG AACTCATCAC TGGCCTCAGC 
TTCTACCAGG ACCGACAAGA GTCAGTTTCA GAACTGCTGA GGTTGAAAAC ACATTTGCCA 
ATCTTCAGCC AAGAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001295256.1
CDS	169..2889
Translation	MERDGDQAGHGPRHGSAGNGRELESPAAASLLAPMDLGEEPLEKAERARPAKDPNTYKVLSLVLSVCVLTTILGCIFGLKPSCAKEVKSCKGRCFERTFSNCRCDAACVSLGNCCLDFQETCVEPTHIWTCNKFRCGEKRLSRFVCSCADDCKTHNDCCINYSSVCQDKKSWVEETCESIDTPECPAEFESPPTLLFSLDGFRAEYLHTWGGLLPVISKLKNCGTYTKNMRPMYPTKTFPNHYSIVTGLYPESHGIIDNKMYDPKMNASFSLKSKEKFNPLWYKGQPIWVTANHQEVKSGTYFWPGSDVEIDGILPDIYKVYNGSVPFEERILAVLEWLQLPSHERPHFYTLYLEEPDSSGHSHGPVSSEVIKALQKVDRLVGMLMDGLKDLGLDKCLNLILISDHGMEQGSCKKYVYLNKYLGDVNNVKVVYGPAARLRPTDVPETYYSFNYEALAKNLSCREPNQHFRPYLKPFLPKRLHFAKSDRIEPLTFYLDPQWQLALNPSERKYCGSGFHGSDNLFSNMQALFIGYGPAFKHGAEVDSFENIEVYNLMCDLLGLIPAPNNGSHGSLNHLLKKPIYNPSHPKEEGFLSQCPIKSTSNDLGCTCDPWIVPIKDFEKQLNLTTEDVDDIYHMTVPYGRPRILLKQHRVCLLQQQQFLTGYSLDLLMPLWASYTFLSNDQFSRDDFSNCLYQDLRIPLSPVHKCSYYKSNSKLSYGFLTPPRLNRVSNHIYSEALLTSNIVPMYQSFQVIWHYLHDTLLQRYAHERNGINVVSGPVFDFDYDGRYDSLEILKQNSRVIRSQEILIPTHFFIVLTSCKQLSETPLECSALESSAYILPHRPDNIESCTHGKRESSWVEELLTLHRARVTDVELITGLSFYQDRQESVSELLRLKTHLPIFSQED

Target ORF information:

RefSeq Version	NM_001308327.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308327.1

ORF Insert Sequence:

ATGGAGCGCG ACGGCGACCA GGCAGGGCAC GGGCCCCGGC ACGGGTCAGC GGGAAACGGT 
CGCGAGCTCG AGTCACCAGC CGCGGCTTCG CTGCTCGCGC CCATGGACCT AGGAGAGGAG 
CCGCTGGAGA AGGCGGAGCG TGCCCGCCCT GCCAAAGACC CCAACACCTA CAAAGTGCTG 
TCGCTGGTTT TGTCAGTATG TGTGCTAACA ACAATTCTTG GTTGTATATT TGGGTTGAAA 
CCAAGCTGCG CCAAAGAAGT AAAAAGTTGC AAAGGCCGCT GCTTTGAAAG GACGTTCAGC 
AACTGTCGCT GTGATGCTGC CTGTGTCAGC CTTGGGAACT GCTGTCTGGA TTTCCAGGAG 
ACCTGTGTGG AACCAACACA TATATGGACT TGCAACAAAT TCAGGTGCGG TGAGAAGAGG 
CTGTCCAGGT TCGTGTGCTC CTGTGCAGAC GACTGCAAAA CCCACAATGA CTGTTGCATC 
AACTACAGTT CTGTGTGCCA AGATAAGAAG AGTTGGGTAG AAGAAACATG TGAAAGCATC 
GATACCCCGG AGTGTCCAGC AGAGTTTGAA TCACCCCCTA CTCTCTTGTT TTCTTTGGAT 
GGATTCAGAG CTGAGTATTT GCACACCTGG GGTGGACTTC TTCCTGTCAT TAGCAAGCTG 
AAAAACTGTG GAACATACAC TAAAAACATG AGGCCTATGT ACCCTACCAA GACGTTTCCC 
AATCATTACA GCATCGTCAC AGGACTTTAT CCGGAATCCC ATGGCATAAT TGATAACAAG 
ATGTATGATC CCAAAATGAA TGCTTCTTTC TCGCTTAAAA GTAAAGAGAA ATTCAACCCT 
TTGTGGTACA AAGGACAGCC GATTTGGGTG ACCGCTAATC ATCAGGAGGT CAAGTCCGGC 
ACATACTTTT GGCCAGGATC AGATGTGGAG ATTGACGGGA TTCTACCAGA TATCTACAAA 
GTGTATAATG GGTCAGTACC ATTTGAAGAA AGGATTTTAG CTGTTCTTGA GTGGCTGCAG 
CTTCCTAGCC ATGAAAGACC ACACTTTTAC ACTCTGTATT TAGAAGAACC AGATTCTTCA 
GGGCATTCAC ATGGACCAGT CAGCAGCGAG GTCATCAAGG CCTTGCAGAA GGTTGACCGC 
CTGGTCGGCA TGCTGATGGA CGGCCTGAAG GACCTGGGCT TGGATAAATG CCTGAACCTC 
ATCCTCATTT CAGATCACGG CATGGAACAA GGCAGCTGTA AGAAGTATGT GTACCTGAAT 
AAGTACTTGG GGGATGTGAA CAATGTGAAA GTTGTGTATG GACCTGCTGC TCGGTTGAGA 
CCCACTGATG TTCCAGAGAC ATACTATTCA TTTAACTATG AAGCCCTTGC AAAAAATCTT 
TCTTGCCGGG AGCCAAACCA GCATTTCCGG CCTTACCTGA AACCCTTCTT ACCCAAGCGC 
TTACACTTCG CTAAAAGTGA CCGGATTGAG CCCCTGACCT TCTACCTGGA CCCTCAGTGG 
CAACTTGCGT TGAATCCATC AGAGAGGAAA TATTGTGGAA GTGGATTTCA TGGCTCTGAC 
AACTTGTTTT CAAACATGCA AGCTCTTTTT ATTGGCTATG GACCTGCCTT CAAGCATGGT 
GCTGAAGTTG ACTCCTTTGA AAATATTGAA GTCTATAACT TAATGTGTGA CTTACTGGGT 
TTGATCCCAG CTCCCAATAA TGGAAGTCAT GGCAGCCTTA ACCACCTTCT AAAGAAACCC 
ATTTACAACC CAAGTCATCC CAAAGAAGAG GGCTTCCTGT CTCAGTGTCC AATCAAATCA 
ACATCCAATG ACCTCGGCTG CACATGTGAC CCTTGGATTG TGCCAATCAA GGACTTTGAG 
AAACAGCTTA ATCTGACCAC AGAAGATGTA GATGACATTT ACCATATGAC TGTACCCTAC 
GGGAGGCCCC GGATTCTACT GAAGCAGCAC CGCGTCTGTC TACTCCAGCA GCAACAGTTC 
TTGACCGGAT ACAGCCTGGA CCTCCTCATG CCCCTCTGGG CGTCTTACAC CTTCCTCAGT 
AATGACCAAT TCTCCAGAGA TGACTTTTCC AACTGTCTGT ACCAGGATCT TCGGATTCCC 
CTTAGTCCTG TGCATAAATG TTCCTATTAC AAAAGCAATT CTAAGCTAAG TTATGGGTTC 
CTCACGCCAC CGAGACTAAA TAGAGTTTCA AATCACATAT ACTCTGAAGC GCTGCTTACA 
TCTAATATAG TGCCAATGTA TCAGAGTTTT CAAGTTATAT GGCACTACCT TCATGACACC 
CTACTGCAAA GGTATGCCCA TGAAAGGAAC GGCATCAATG TTGTCAGCGG TCCCGTGTTT 
GACTTTGATT ATGACGGACG CTATGATTCC TTAGAGATCC TGAAACAAAA CAGCAGAGTC 
ATCCGAAGCC AGGAAATTCT GATTCCGACT CACTTCTTCA TTGTGCTCAC CAGCTGCAAG 
CAGCTGTCTG AGACTCCCTT GGAGTGCTCC GCCTTAGAGT CCTCAGCTTA CATACTGCCT 
CATAGGCCTG ATAACATTGA GAGCTGTACG CATGGGAAGC GGGAGTCTTC ATGGGTCGAG 
GAGTTGCTGA CCTTGCACAG AGCTCGGGTC ACAGACGTGG AACTCATCAC TGGCCTCAGC 
TTCTACCAGG ACCGACAAGA GTCAGTTTCA GAACTGCTGA GGTTGAAAAC ACATTTGCCA 
ATCTTCAGCC AAGAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Enpp1 ( XM_006512598.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512661.1 Mus musculus ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), transcript variant X1, mRNA.

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CloneID	OMu43766
Clone ID Related Accession (Same CDS sequence)	XM_006512598.4 , XM_006512598.3
Accession Version	XM_006512598.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	ectonucleotide pyrophosphatase/phosphodiesterase family member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512598.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTGAAC ACAAGAGGAG AGTAGATAAC TGTGCTCTCT GCAGATCTCC CCAACAGATC 
TCTCATTTGA AGGACATGAC AAAGGTTTTG TCAGTATGTG TGCTAACAAC AATTCTTGGT 
TGTATATTTG GGTTGAAACC AAGCTGCGCC AAAGAAGTAA AAAGTTGCAA AGGCCGCTGC 
TTTGAAAGGA CGTTCAGCAA CTGTCGCTGT GATGCTGCCT GTGTCAGCCT TGGGAACTGC 
TGTCTGGATT TCCAGGAGAC CTGTGTGGAA CCAACACATA TATGGACTTG CAACAAATTC 
AGGTGCGGTG AGAAGAGGCT GTCCAGGTTC GTGTGCTCCT GTGCAGACGA CTGCAAAACC 
CACAATGACT GTTGCATCAA CTACAGTTCT GTGTGCCAAG ATAAGAAGAG TTGGGTAGAA 
GAAACATGTG AAAGCATCGA TACCCCGGAG TGTCCAGCAG AGTTTGAATC ACCCCCTACT 
CTCTTGTTTT CTTTGGATGG ATTCAGAGCT GAGTATTTGC ACACCTGGGG TGGACTTCTT 
CCTGTCATTA GCAAGCTGAA AAACTGTGGA ACATACACTA AAAACATGAG GCCTATGTAC 
CCTACCAAGA CGTTTCCCAA TCATTACAGC ATCGTCACAG GACTTTATCC GGAATCCCAT 
GGCATAATTG ATAACAAGAT GTATGATCCC AAAATGAATG CTTCTTTCTC GCTTAAAAGT 
AAAGAGAAAT TCAACCCTTT GTGGTACAAA GGACAGCCGA TTTGGGTGAC CGCTAATCAT 
CAGGAGGTCA AGTCCGGCAC ATACTTTTGG CCAGGATCAG ATGTGGAGAT TGACGGGATT 
CTACCAGATA TCTACAAAGT GTATAATGGG TCAGTACCAT TTGAAGAAAG GATTTTAGCT 
GTTCTTGAGT GGCTGCAGCT TCCTAGCCAT GAAAGACCAC ACTTTTACAC TCTGTATTTA 
GAAGAACCAG ATTCTTCAGG GCATTCACAT GGACCAGTCA GCAGCGAGGT CATCAAGGCC 
TTGCAGAAGG TTGACCGCCT GGTCGGCATG CTGATGGACG GCCTGAAGGA CCTGGGCTTG 
GATAAATGCC TGAACCTCAT CCTCATTTCA GATCACGGCA TGGAACAAGG CAGCTGTAAG 
AAGTATGTGT ACCTGAATAA GTACTTGGGG GATGTGAACA ATGTGAAAGT TGTGTATGGA 
CCTGCTGCTC GGTTGAGACC CACTGATGTT CCAGAGACAT ACTATTCATT TAACTATGAA 
GCCCTTGCAA AAAATCTTTC TTGCCGGGAG CCAAACCAGC ATTTCCGGCC TTACCTGAAA 
CCCTTCTTAC CCAAGCGCTT ACACTTCGCT AAAAGTGACC GGATTGAGCC CCTGACCTTC 
TACCTGGACC CTCAGTGGCA ACTTGCGTTG AATCCATCAG AGAGGAAATA TTGTGGAAGT 
GGATTTCATG GCTCTGACAA CTTGTTTTCA AACATGCAAG CTCTTTTTAT TGGCTATGGA 
CCTGCCTTCA AGCATGGTGC TGAAGTTGAC TCCTTTGAAA ATATTGAAGT CTATAACTTA 
ATGTGTGACT TACTGGGTTT GATCCCAGCT CCCAATAATG GAAGTCATGG CAGCCTTAAC 
CACCTTCTAA AGAAACCCAT TTACAACCCA AGTCATCCCA AAGAAGAGGG CTTCCTGTCT 
CAGTGTCCAA TCAAATCAAC ATCCAATGAC CTCGGCTGCA CATGTGACCC TTGGATTGTG 
CCAATCAAGG ACTTTGAGAA ACAGCTTAAT CTGACCACAG AAGATGTAGA TGACATTTAC 
CATATGACTG TACCCTACGG GAGGCCCCGG ATTCTACTGA AGCAGCACCG CGTCTGTCTA 
CTCCAGCAGC AACAGTTCTT GACCGGATAC AGCCTGGACC TCCTCATGCC CCTCTGGGCG 
TCTTACACCT TCCTCAGTAA TGACCAATTC TCCAGAGATG ACTTTTCCAA CTGTCTGTAC 
CAGGATCTTC GGATTCCCCT TAGTCCTGTG CATAAATGTT CCTATTACAA AAGCAATTCT 
AAGCTAAGTT ATGGGTTCCT CACGCCACCG AGACTAAATA GAGTTTCAAA TCACATATAC 
TCTGAAGCGC TGCTTACATC TAATATAGTG CCAATGTATC AGAGTTTTCA AGTTATATGG 
CACTACCTTC ATGACACCCT ACTGCAAAGG TATGCCCATG AAAGGAACGG CATCAATGTT 
GTCAGCGGTC CCGTGTTTGA CTTTGATTAT GACGGACGCT ATGATTCCTT AGAGATCCTG 
AAACAAAACA GCAGAGTCAT CCGAAGCCAG GAAATTCTGA TTCCGACTCA CTTCTTCATT 
GTGCTCACCA GCTGCAAGCA GCTGTCTGAG ACTCCCTTGG AGTGCTCCGC CTTAGAGTCC 
TCAGCTTACA TACTGCCTCA TAGGCCTGAT AACATTGAGA GCTGTACGCA TGGGAAGCGG 
GAGTCTTCAT GGGTCGAGGA GTTGCTGACC TTGCACAGAG CTCGGGTCAC AGACGTGGAA 
CTCATCACTG GCCTCAGCTT CTACCAGGAC CGACAAGAGT CAGTTTCAGA ACTGCTGAGG 
TTGAAAACAC ATTTGCCAAT CTTCAGCCAA GAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512661.1
CDS	15..2633
Translation	MIEHKRRVDNCALCRSPQQISHLKDMTKVLSVCVLTTILGCIFGLKPSCAKEVKSCKGRCFERTFSNCRCDAACVSLGNCCLDFQETCVEPTHIWTCNKFRCGEKRLSRFVCSCADDCKTHNDCCINYSSVCQDKKSWVEETCESIDTPECPAEFESPPTLLFSLDGFRAEYLHTWGGLLPVISKLKNCGTYTKNMRPMYPTKTFPNHYSIVTGLYPESHGIIDNKMYDPKMNASFSLKSKEKFNPLWYKGQPIWVTANHQEVKSGTYFWPGSDVEIDGILPDIYKVYNGSVPFEERILAVLEWLQLPSHERPHFYTLYLEEPDSSGHSHGPVSSEVIKALQKVDRLVGMLMDGLKDLGLDKCLNLILISDHGMEQGSCKKYVYLNKYLGDVNNVKVVYGPAARLRPTDVPETYYSFNYEALAKNLSCREPNQHFRPYLKPFLPKRLHFAKSDRIEPLTFYLDPQWQLALNPSERKYCGSGFHGSDNLFSNMQALFIGYGPAFKHGAEVDSFENIEVYNLMCDLLGLIPAPNNGSHGSLNHLLKKPIYNPSHPKEEGFLSQCPIKSTSNDLGCTCDPWIVPIKDFEKQLNLTTEDVDDIYHMTVPYGRPRILLKQHRVCLLQQQQFLTGYSLDLLMPLWASYTFLSNDQFSRDDFSNCLYQDLRIPLSPVHKCSYYKSNSKLSYGFLTPPRLNRVSNHIYSEALLTSNIVPMYQSFQVIWHYLHDTLLQRYAHERNGINVVSGPVFDFDYDGRYDSLEILKQNSRVIRSQEILIPTHFFIVLTSCKQLSETPLECSALESSAYILPHRPDNIESCTHGKRESSWVEELLTLHRARVTDVELITGLSFYQDRQESVSELLRLKTHLPIFSQED

Target ORF information:

RefSeq Version	XM_006512598.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512598.4

ORF Insert Sequence:

ATGATTGAAC ACAAGAGGAG AGTAGATAAC TGTGCTCTCT GCAGATCTCC CCAACAGATC 
TCTCATTTGA AGGACATGAC AAAGGTTTTG TCAGTATGTG TGCTAACAAC AATTCTTGGT 
TGTATATTTG GGTTGAAACC AAGCTGCGCC AAAGAAGTAA AAAGTTGCAA AGGCCGCTGC 
TTTGAAAGGA CGTTCAGCAA CTGTCGCTGT GATGCTGCCT GTGTCAGCCT TGGGAACTGC 
TGTCTGGATT TCCAGGAGAC CTGTGTGGAA CCAACACATA TATGGACTTG CAACAAATTC 
AGGTGCGGTG AGAAGAGGCT GTCCAGGTTC GTGTGCTCCT GTGCAGACGA CTGCAAAACC 
CACAATGACT GTTGCATCAA CTACAGTTCT GTGTGCCAAG ATAAGAAGAG TTGGGTAGAA 
GAAACATGTG AAAGCATCGA TACCCCGGAG TGTCCAGCAG AGTTTGAATC ACCCCCTACT 
CTCTTGTTTT CTTTGGATGG ATTCAGAGCT GAGTATTTGC ACACCTGGGG TGGACTTCTT 
CCTGTCATTA GCAAGCTGAA AAACTGTGGA ACATACACTA AAAACATGAG GCCTATGTAC 
CCTACCAAGA CGTTTCCCAA TCATTACAGC ATCGTCACAG GACTTTATCC GGAATCCCAT 
GGCATAATTG ATAACAAGAT GTATGATCCC AAAATGAATG CTTCTTTCTC GCTTAAAAGT 
AAAGAGAAAT TCAACCCTTT GTGGTACAAA GGACAGCCGA TTTGGGTGAC CGCTAATCAT 
CAGGAGGTCA AGTCCGGCAC ATACTTTTGG CCAGGATCAG ATGTGGAGAT TGACGGGATT 
CTACCAGATA TCTACAAAGT GTATAATGGG TCAGTACCAT TTGAAGAAAG GATTTTAGCT 
GTTCTTGAGT GGCTGCAGCT TCCTAGCCAT GAAAGACCAC ACTTTTACAC TCTGTATTTA 
GAAGAACCAG ATTCTTCAGG GCATTCACAT GGACCAGTCA GCAGCGAGGT CATCAAGGCC 
TTGCAGAAGG TTGACCGCCT GGTCGGCATG CTGATGGACG GCCTGAAGGA CCTGGGCTTG 
GATAAATGCC TGAACCTCAT CCTCATTTCA GATCACGGCA TGGAACAAGG CAGCTGTAAG 
AAGTATGTGT ACCTGAATAA GTACTTGGGG GATGTGAACA ATGTGAAAGT TGTGTATGGA 
CCTGCTGCTC GGTTGAGACC CACTGATGTT CCAGAGACAT ACTATTCATT TAACTATGAA 
GCCCTTGCAA AAAATCTTTC TTGCCGGGAG CCAAACCAGC ATTTCCGGCC TTACCTGAAA 
CCCTTCTTAC CCAAGCGCTT ACACTTCGCT AAAAGTGACC GGATTGAGCC CCTGACCTTC 
TACCTGGACC CTCAGTGGCA ACTTGCGTTG AATCCATCAG AGAGGAAATA TTGTGGAAGT 
GGATTTCATG GCTCTGACAA CTTGTTTTCA AACATGCAAG CTCTTTTTAT TGGCTATGGA 
CCTGCCTTCA AGCATGGTGC TGAAGTTGAC TCCTTTGAAA ATATTGAAGT CTATAACTTA 
ATGTGTGACT TACTGGGTTT GATCCCAGCT CCCAATAATG GAAGTCATGG CAGCCTTAAC 
CACCTTCTAA AGAAACCCAT TTACAACCCA AGTCATCCCA AAGAAGAGGG CTTCCTGTCT 
CAGTGTCCAA TCAAATCAAC ATCCAATGAC CTCGGCTGCA CATGTGACCC TTGGATTGTG 
CCAATCAAGG ACTTTGAGAA ACAGCTTAAT CTGACCACAG AAGATGTAGA TGACATTTAC 
CATATGACTG TACCCTACGG GAGGCCCCGG ATTCTACTGA AGCAGCACCG CGTCTGTCTA 
CTCCAGCAGC AACAGTTCTT GACCGGATAC AGCCTGGACC TCCTCATGCC CCTCTGGGCG 
TCTTACACCT TCCTCAGTAA TGACCAATTC TCCAGAGATG ACTTTTCCAA CTGTCTGTAC 
CAGGATCTTC GGATTCCCCT TAGTCCTGTG CATAAATGTT CCTATTACAA AAGCAATTCT 
AAGCTAAGTT ATGGGTTCCT CACGCCACCG AGACTAAATA GAGTTTCAAA TCACATATAC 
TCTGAAGCGC TGCTTACATC TAATATAGTG CCAATGTATC AGAGTTTTCA AGTTATATGG 
CACTACCTTC ATGACACCCT ACTGCAAAGG TATGCCCATG AAAGGAACGG CATCAATGTT 
GTCAGCGGTC CCGTGTTTGA CTTTGATTAT GACGGACGCT ATGATTCCTT AGAGATCCTG 
AAACAAAACA GCAGAGTCAT CCGAAGCCAG GAAATTCTGA TTCCGACTCA CTTCTTCATT 
GTGCTCACCA GCTGCAAGCA GCTGTCTGAG ACTCCCTTGG AGTGCTCCGC CTTAGAGTCC 
TCAGCTTACA TACTGCCTCA TAGGCCTGAT AACATTGAGA GCTGTACGCA TGGGAAGCGG 
GAGTCTTCAT GGGTCGAGGA GTTGCTGACC TTGCACAGAG CTCGGGTCAC AGACGTGGAA 
CTCATCACTG GCCTCAGCTT CTACCAGGAC CGACAAGAGT CAGTTTCAGA ACTGCTGAGG 
TTGAAAACAC ATTTGCCAAT CTTCAGCCAA GAAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Enpp1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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