Enpp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Enpp1
Entrez Gene ID	85496
Full Name	ectonucleotide pyrophosphatase/phosphodiesterase 1
Synonyms	Npps,Pc1
General protein information	Preferred Names ectonucleotide pyrophosphatase/phosphodiesterase 1 Names ectonucleotide pyrophosphatase/phosphodiesterase family member 1 E-NPP 1 phosphodiesterase I/nucleotide pyrophosphatase 1 plasma-cell membrane glycoprotein PC-1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1p12
Summary	mutation of the human homolog is detected in patients with idiopathic infantile arterial calcification [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006227692.3	XP_006227754.1	ectonucleotide pyrophosphatase/phosphodiesterase family member 1 isoform X1
NM_053535.1	NP_445987.1	ectonucleotide pyrophosphatase/phosphodiesterase family member 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ENPP1	NP_006199.2
	ENPP1	XP_001103359.1
Bos taurus (cattle)	ENPP1	NP_001193141.1
Mus musculus (house mouse)	Enpp1	NP_032839.3
Rattus norvegicus (Norway rat)	Enpp1	NP_445987.1
Pan troglodytes (chimpanzee)	ENPP1	XP_518743.3
Canis lupus familiaris (dog)	ENPP1	XP_003638833.1
Caenorhabditis elegans (roundworm)	CELE_C27A7.3	NP_001041087.1
Xenopus tropicalis (tropical clawed frog)	enpp1	XP_002936360.2
Caenorhabditis elegans (roundworm)	CELE_C27A7.1	NP_001041086.1
Gallus gallus (chicken)	ENPP1	XP_424539.4
Danio rerio (zebrafish)	enpp1	NP_001025339.1
Arabidopsis thaliana (thale cress)	AT4G29680	NP_194697.1
Oryza sativa (rice)	Os01g0196600	NP_001042295.1

Gene Ontology
Bibliography

Related articles in PubMed

ENPP1 Mutation Causes Recessive Cole Disease by Altering Melanogenesis.
Chourabi M, Liew MS, Lim S, H'mida-Ben Brahim D, Boussofara L, Dai L, Wong PM, Foo JN, Sriha B, Robinson KS, Denil S, Common JE, Mama? O, Ben Khalifa Y, Bollen M, Liu J, Denguezli M, Bonnard C, Saad A, Reversade B
The Journal of investigative dermatology(2017 Sep)

Effects of Different Variants in the ENPP1 Gene on the Functional Properties of Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 1.
Stella J, Buers I, van de Wetering K, H?hne W, Rutsch F, Nitschke Y
Human mutation37(11)1190-1201(2016 11)

Unilateral anterior crossbite induces aberrant mineral deposition in degenerative temporomandibular cartilage in rats.
Zhang M, Wang H, Zhang J, Zhang H, Yang H, Wan X, Jing L, Lu L, Liu X, Yu S, Chang W, Wang M
Osteoarthritis and cartilage24(5)921-31(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Increased NPP1 expression and activity might contribute to the decreased mineralisation observed when osteoblasts are exposed to acid conditions.
Title: Acidosis is a key regulator of osteoblast ecto-nucleotidase pyrophosphatase/phosphodiesterase 1 (NPP1) expression and activity.

Cyclic mechanical tension increases the expression of ENPP-1 in end-plate chondrocytes in a manner likely dependent on TGF-beta induction by the ERK1/2 signaling pathway.
Title: Expression of ectonucleotide pyrophosphatase-1 in end-plate chondrocytes with transforming growth factor beta 1 siRNA interference by cyclic mechanical tension.

The down-regulation of the ENPP-1 gene expression induced by intermittent cyclic mechanical tension is likely dependent on TGF-beta1 in end-plate chondrocytes of lumbar vertebrae.
Title: Intermittent cyclic mechanical tension-induced down-regulation of ectonucleotide pyrophosphatase phosphodiesterase 1 gene expression is mainly dependent on TGF-?1 in end-plate chondrocytes.

ecto-nucleotide pyrophosphatase/phosphodiesterase 1 and 3 are expressed on podocytes, but changes in expression of these enzymes are most likely not involved in etiology of insulin resistance in podocytes.
Title: Expression of membrane-bound NPP-type ecto-phosphodiesterases in rat podocytes cultured at normal and high glucose concentrations.

The effects of mechanical strain on the progressive ankylosis (ANK) gene and extracellular nucleotide phosphatase/phosphodiesterase (ENPP)1 mRNA expression and TGF-beta1 protein expression in rat endplate chondrocytes, were investigated.
Title: Effects of mechanical strain on ANK, ENPP1 and TGF-?1 expression in rat endplate chondrocytes in vitro.

The following Enpp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Enpp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13515	NM_053535.1 Latest version!	Rattus norvegicus ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$531.30 $759.00
ORa22042	XM_006227692.3 Latest version!	Rattus norvegicus ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Enpp1 ( NM_053535.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_445987.1 Rattus norvegicus ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), mRNA.

Price & Availability↑

CloneID	ORa13515
Clone ID Related Accession (Same CDS sequence)	NM_053535.1
Accession Version	NM_053535.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Rattus norvegicus(Norway rat)
Product	ectonucleotide pyrophosphatase/phosphodiesterase family member 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF340186.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF340185.1, AF340186.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMEA2689594 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCGCG ACGGCGAACA GGCAGGGCAA GGGCCCCGGC ATGGGCCAGC GGGAAACGGC 
CGCGAGCTGG AGTCTCCAGC CGCCGCTTCG CTGCTGGCGC CCATGGACCT AGGGGAGGAG 
CCGCTGGAGA AGGCGGAGCG TGCGCGCACC GCCAAAGACC CCAACACCTA CAAAGTGCTG 
TCGCTGGTTT TGTCAGTATG CGTGCTAACA ACTATTCTTG GTTGTATCTT TGGGTTGAAA 
CCAAGCTGTG CCAAAGAAGT GAAAAGTTGC AAAGGCCGCT GCTTTGAAAG GACGTTCAGC 
AATTGTCGCT GCGATGCTGC CTGTGTCAGC CTTGGGAACT GCTGTCTGGA TTTCCAAGAG 
ACCTGTGTGG AACCAACACA TATATGGACT TGCAACAAGT TCAGGTGCGG CGAGAAGAGG 
CTGTCCAGAT TTGTGTGCTC CTGTGCGGAC GACTGCAAAG CCCACAATGA CTGTTGCATC 
AACTACAGTT CAGTGTGCCA AGAAAAGAAG AGTTGGGTAG AAGAAGCCTG CGAAACCATC 
GATGCACCAC AGTGTCCAGC AGAGTTTGAA TCACCCCCTA CTCTCTTGTT TTCTTTGGAT 
GGATTCAGAG CTGAATATTT GCACACTTGG GGTGGACTTC TTCCTGTCAT TAGCAAGCTG 
AAAAACTGTG GAACGTACAC TAAAAACATG AGGCCTGTGT ACCCTACCAA GACGTTTCCC 
AATCATTACA GCATCGTCAC AGGACTTTAT CCAGAATCAC ATGGCATAAT TGACAACAAG 
ATGTATGATC CTAAAATGAA CGCTTCTTTC TCACTTAAAA GTAAAGAGAA ATTCAACCCT 
CTGTGGTACA AAGGACAGCC GATCTGGGTG ACCGCTAATC ATCAGGAGGT CAGGTCTGGC 
ACATACTTCT GGCCAGGATC AGACGTGGAA ATTGATGGGA TTCTGCCAGA TATCTACAAA 
GTGTATAATG GGTCAGTACC ATTTGAAGAA AGGATTTTAG CTGTTCTCGA GTGGCTACAG 
CTTCCTAGCT ATGAAAGACC ACACTTTTAC ACTCTGTATT TAGAAGAACC AGATTCTTCA 
GGGCATTCAC ACGGACCAGT AAGCAGTGAG GTCATCAAGG CCCTGCAGAA GGTTGACCAC 
ATAGTTGGCA TGCTGATGGA TGGCCTGAAG GACCTGGGCT TGGATAAATG CCTGAACCTC 
ATCCTCATTT CAGATCACGG CATGGAACAA GGCAGTTGTA AGAAGTACGT GTACCTGAAT 
AAGTACCTGG GGGATGTGAA CAATGTCAAG GTTGTGTATG GACCTGCTGC TCGATTGAGA 
CCCACCGAGG TTCCGGAGAC ATACTATTCG TTTAACTATG AAGCCCTTGC AAAAAATCTT 
TCTTGCCGGG AAACAAACCA GCACTTCCGG CCTTATCTGA AACACTTCTT ACCCAAGCGC 
TTACACTTTG CTAAAAATGA CAGGATTGAG CCACTGACCT TCTATCTGGA CCCTCAATGG 
CAACTTGCCT TGAATCCGTC AGAAAGGAAA TATTGTGGAA GTGGATTTCA TGGCTCTGAC 
AACCTGTTTT CAAACATGCA AGCTCTCTTC ATTGGCTATG GACCTGCCTT CAAGCACGGT 
GCTGAGGTTG ACTCCTTTGA AAATATCGAG GTCTATAACT TAATGTGTGA TTTATTGGGT 
TTGATCCCAG CTCCTAATAA TGAAAGTCAC GGCAGTCTCA ACCATCTTCT AAAGAAACCC 
ATTTATACCC CAAGTCATCC CAAAGAAGAG AGCTTCCTGT CCCAGTGTCC AATCAAATCA 
GTGTCCAGTG ACCTCGGCTG CACATGTGAC CCTTCGATTG TGCCGATCAT GGACTTCGAG 
AAGCAGTTCA ATCTGACCAC CGATGCTGTA GAGGATGTTT ACTCTATGAC TGTGCCCAAC 
GGAAGGCCCC GGAATCTGCA GAAGCAGCAC CGCGTCTGTC TGCTCCATCA GCAACAGTTT 
TTGACTGGGT ACAGCCTGGA CCTCCTCATG CCCCTGTGGA CGTCTTACAC CTTTCTCAGT 
AATGACCAGT TCTCCACAGA TGACTTTTCC AACTGTTTGT ACCAGGACCT TCGGATTCCC 
CTTAGTCCCA TGCATAAATG TTCCTATTAT AAAAGCACTT CTAAGCTCAG TTATGGGTTC 
CTTACACCGC CAAGACTAAA CAGAGTCTCA CGTCAAATAT ATTCTGAAGC CTTGCTTACT 
TCTAACATAG TGCCAATGTA CCAGAGTTTT CAAGTTATAT GGCAATACCT TCATGACACC 
GTACTGCGAA GGTATGCCCA AGAAAGGAAT GGCGTCAATG TTGTCAGCGG CCCCGTGTTC 
GACTTTGATT ATGATGGACG GTATGATTCC TCAGAGATCC TGAAACAAAA CACCAGAGTC 
ATCCGCAGTC AAGAAAATCT GATTCCCACT CACTTCTTCA TTGTGCTTAC CAGCTGCAAG 
CAGCTGTCTG AGAGCCCCTT AAAGTGTACA GCCTTAGAGT CTTCAGCCTT CCTCTTGCCT 
CACAGGCCTG ATAACATCGA GAGCTGTACA CATGGAAAGC AGGAGTCTGC ATGGGTTGAA 
GAGTTGCTGG CATTGCACAG AGCTCGGGTC ACAGACGTTG AGCTCATCAC GGGTCTCAGC 
TTCTACCAGG ACCGACAGGA GTCAGTTTCA GAACTGCTGA GGTTGAAAAC ACACTTACCA 
ATCTTCAGCC AAGAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_445987.1
CDS	1..2721
Translation	MERDGEQAGQGPRHGPAGNGRELESPAAASLLAPMDLGEEPLEKAERARTAKDPNTYKVLSLVLSVCVLTTILGCIFGLKPSCAKEVKSCKGRCFERTFSNCRCDAACVSLGNCCLDFQETCVEPTHIWTCNKFRCGEKRLSRFVCSCADDCKAHNDCCINYSSVCQEKKSWVEEACETIDAPQCPAEFESPPTLLFSLDGFRAEYLHTWGGLLPVISKLKNCGTYTKNMRPVYPTKTFPNHYSIVTGLYPESHGIIDNKMYDPKMNASFSLKSKEKFNPLWYKGQPIWVTANHQEVRSGTYFWPGSDVEIDGILPDIYKVYNGSVPFEERILAVLEWLQLPSYERPHFYTLYLEEPDSSGHSHGPVSSEVIKALQKVDHIVGMLMDGLKDLGLDKCLNLILISDHGMEQGSCKKYVYLNKYLGDVNNVKVVYGPAARLRPTEVPETYYSFNYEALAKNLSCRETNQHFRPYLKHFLPKRLHFAKNDRIEPLTFYLDPQWQLALNPSERKYCGSGFHGSDNLFSNMQALFIGYGPAFKHGAEVDSFENIEVYNLMCDLLGLIPAPNNESHGSLNHLLKKPIYTPSHPKEESFLSQCPIKSVSSDLGCTCDPSIVPIMDFEKQFNLTTDAVEDVYSMTVPNGRPRNLQKQHRVCLLHQQQFLTGYSLDLLMPLWTSYTFLSNDQFSTDDFSNCLYQDLRIPLSPMHKCSYYKSTSKLSYGFLTPPRLNRVSRQIYSEALLTSNIVPMYQSFQVIWQYLHDTVLRRYAQERNGVNVVSGPVFDFDYDGRYDSSEILKQNTRVIRSQENLIPTHFFIVLTSCKQLSESPLKCTALESSAFLLPHRPDNIESCTHGKQESAWVEELLALHRARVTDVELITGLSFYQDRQESVSELLRLKTHLPIFSQED

Target ORF information:

RefSeq Version	NM_053535.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053535.1

ORF Insert Sequence:

ATGGAGCGCG ACGGCGAACA GGCAGGGCAA GGGCCCCGGC ATGGGCCAGC GGGAAACGGC 
CGCGAGCTGG AGTCTCCAGC CGCCGCTTCG CTGCTGGCGC CCATGGACCT AGGGGAGGAG 
CCGCTGGAGA AGGCGGAGCG TGCGCGCACC GCCAAAGACC CCAACACCTA CAAAGTGCTG 
TCGCTGGTTT TGTCAGTATG CGTGCTAACA ACTATTCTTG GTTGTATCTT TGGGTTGAAA 
CCAAGCTGTG CCAAAGAAGT GAAAAGTTGC AAAGGCCGCT GCTTTGAAAG GACGTTCAGC 
AATTGTCGCT GCGATGCTGC CTGTGTCAGC CTTGGGAACT GCTGTCTGGA TTTCCAAGAG 
ACCTGTGTGG AACCAACACA TATATGGACT TGCAACAAGT TCAGGTGCGG CGAGAAGAGG 
CTGTCCAGAT TTGTGTGCTC CTGTGCGGAC GACTGCAAAG CCCACAATGA CTGTTGCATC 
AACTACAGTT CAGTGTGCCA AGAAAAGAAG AGTTGGGTAG AAGAAGCCTG CGAAACCATC 
GATGCACCAC AGTGTCCAGC AGAGTTTGAA TCACCCCCTA CTCTCTTGTT TTCTTTGGAT 
GGATTCAGAG CTGAATATTT GCACACTTGG GGTGGACTTC TTCCTGTCAT TAGCAAGCTG 
AAAAACTGTG GAACGTACAC TAAAAACATG AGGCCTGTGT ACCCTACCAA GACGTTTCCC 
AATCATTACA GCATCGTCAC AGGACTTTAT CCAGAATCAC ATGGCATAAT TGACAACAAG 
ATGTATGATC CTAAAATGAA CGCTTCTTTC TCACTTAAAA GTAAAGAGAA ATTCAACCCT 
CTGTGGTACA AAGGACAGCC GATCTGGGTG ACCGCTAATC ATCAGGAGGT CAGGTCTGGC 
ACATACTTCT GGCCAGGATC AGACGTGGAA ATTGATGGGA TTCTGCCAGA TATCTACAAA 
GTGTATAATG GGTCAGTACC ATTTGAAGAA AGGATTTTAG CTGTTCTCGA GTGGCTACAG 
CTTCCTAGCT ATGAAAGACC ACACTTTTAC ACTCTGTATT TAGAAGAACC AGATTCTTCA 
GGGCATTCAC ACGGACCAGT AAGCAGTGAG GTCATCAAGG CCCTGCAGAA GGTTGACCAC 
ATAGTTGGCA TGCTGATGGA TGGCCTGAAG GACCTGGGCT TGGATAAATG CCTGAACCTC 
ATCCTCATTT CAGATCACGG CATGGAACAA GGCAGTTGTA AGAAGTACGT GTACCTGAAT 
AAGTACCTGG GGGATGTGAA CAATGTCAAG GTTGTGTATG GACCTGCTGC TCGATTGAGA 
CCCACCGAGG TTCCGGAGAC ATACTATTCG TTTAACTATG AAGCCCTTGC AAAAAATCTT 
TCTTGCCGGG AAACAAACCA GCACTTCCGG CCTTATCTGA AACACTTCTT ACCCAAGCGC 
TTACACTTTG CTAAAAATGA CAGGATTGAG CCACTGACCT TCTATCTGGA CCCTCAATGG 
CAACTTGCCT TGAATCCGTC AGAAAGGAAA TATTGTGGAA GTGGATTTCA TGGCTCTGAC 
AACCTGTTTT CAAACATGCA AGCTCTCTTC ATTGGCTATG GACCTGCCTT CAAGCACGGT 
GCTGAGGTTG ACTCCTTTGA AAATATCGAG GTCTATAACT TAATGTGTGA TTTATTGGGT 
TTGATCCCAG CTCCTAATAA TGAAAGTCAC GGCAGTCTCA ACCATCTTCT AAAGAAACCC 
ATTTATACCC CAAGTCATCC CAAAGAAGAG AGCTTCCTGT CCCAGTGTCC AATCAAATCA 
GTGTCCAGTG ACCTCGGCTG CACATGTGAC CCTTCGATTG TGCCGATCAT GGACTTCGAG 
AAGCAGTTCA ATCTGACCAC CGATGCTGTA GAGGATGTTT ACTCTATGAC TGTGCCCAAC 
GGAAGGCCCC GGAATCTGCA GAAGCAGCAC CGCGTCTGTC TGCTCCATCA GCAACAGTTT 
TTGACTGGGT ACAGCCTGGA CCTCCTCATG CCCCTGTGGA CGTCTTACAC CTTTCTCAGT 
AATGACCAGT TCTCCACAGA TGACTTTTCC AACTGTTTGT ACCAGGACCT TCGGATTCCC 
CTTAGTCCCA TGCATAAATG TTCCTATTAT AAAAGCACTT CTAAGCTCAG TTATGGGTTC 
CTTACACCGC CAAGACTAAA CAGAGTCTCA CGTCAAATAT ATTCTGAAGC CTTGCTTACT 
TCTAACATAG TGCCAATGTA CCAGAGTTTT CAAGTTATAT GGCAATACCT TCATGACACC 
GTACTGCGAA GGTATGCCCA AGAAAGGAAT GGCGTCAATG TTGTCAGCGG CCCCGTGTTC 
GACTTTGATT ATGATGGACG GTATGATTCC TCAGAGATCC TGAAACAAAA CACCAGAGTC 
ATCCGCAGTC AAGAAAATCT GATTCCCACT CACTTCTTCA TTGTGCTTAC CAGCTGCAAG 
CAGCTGTCTG AGAGCCCCTT AAAGTGTACA GCCTTAGAGT CTTCAGCCTT CCTCTTGCCT 
CACAGGCCTG ATAACATCGA GAGCTGTACA CATGGAAAGC AGGAGTCTGC ATGGGTTGAA 
GAGTTGCTGG CATTGCACAG AGCTCGGGTC ACAGACGTTG AGCTCATCAC GGGTCTCAGC 
TTCTACCAGG ACCGACAGGA GTCAGTTTCA GAACTGCTGA GGTTGAAAAC ACACTTACCA 
ATCTTCAGCC AAGAAGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Enpp1 ( XM_006227692.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006227754.1 Rattus norvegicus ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa22042
Clone ID Related Accession (Same CDS sequence)	XM_006227692.3
Accession Version	XM_006227692.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ectonucleotide pyrophosphatase/phosphodiesterase family member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006227692.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGCG ACGGCGAACA GGCAGGGCAA GGGCCCCGGC ATGGGCCAGC GGGAAACGGC 
CGCGAGCTGG AGTCTCCAGC CGCCGCTTCG CTGCTGGCGC CCATGGACCT AGGGGAGGAG 
CCGCTGGAGA AGGCGGAGCG TGCGCGCACC GCCAAAGACC CCAACACCTA CAAAGTGCTG 
TCGCTGGTTT TGTCAGTATG CGTGCTAACA ACTATTCTTG GTTGTATCTT TGGGTTGAAA 
CCAAGCTGTG CCAAAGAAGT GAAAAGTTGC AAAGGCCGCT GCTTTGAAAG GACGTTCAGC 
AATTGTCGCT GCGATGCTGC CTGTGTCAGC CTTGGGAACT GCTGTCTGGA TTTCCAAGAG 
ACCTGTGTGG AACCAACACA TATATGGACT TGCAACAAGT TCAGGTGCGG CGAGAAGAGG 
CTGTCCAGAT TTGTGTGCTC CTGTGCGGAC GACTGCAAAG CCCACAATGA CTGTTGCATC 
AACTACAGTT CAGTGTGCCA AGAAAAGAAG AGTTGGGTAG AAGAAGCCTG CGAAACCATC 
GATGCACCAC AGTGTCCAGC AGAGTTTGAA TCACCCCCTA CTCTCTTGTT TTCTTTGGAT 
GGATTCAGAG CTGAATATTT GCACACTTGG GGTGGACTTC TTCCTGTCAT TAGCAAGCTG 
AAAAACTGTG GAACGTACAC TAAAAACATG AGGCCTGTGT ACCCTACCAA GACGTTTCCC 
AATCATTACA GCATCGTCAC AGGACTTTAT CCAGAATCAC ATGGCATAAT TGACAACAAG 
ATGTATGATC CTAAAATGAA CGCTTCTTTC TCACTTAAAA GTAAAGAGAA ATTCAACCCT 
CTGTGGTACA AAGGACAGCC GATCTGGGTG ACCGCTAATC ATCAGGAGGT CAGGTCTGGC 
ACATACTTCT GGCCAGGATC AGACGTGGAA ATTGATGGGA TTCTGCCAGA TATCTACAAA 
GTGTATAATG GGTCAGTACC ATTTGAAGAA AGGATTTTAG CTGTTCTCGA GTGGCTACAG 
CTTCCTAGCT ATGAAAGACC ACACTTTTAC ACTCTGTATT TAGAAGAACC AGATTCTTCA 
GGGCATTCAC ACGGACCAGT AAGCAGTGAG GTCATCAAGG CCCTGCAGAA GGTTGACCAC 
ATAGTTGGCA TGCTGATGGA TGGCCTGAAG GACCTGGGCT TGGATAAATG CCTGAACCTC 
ATTCTCATTT CAGATCACGG CATGGAACAA GGCAGTTGTA AGAAGTACGT GTACCTGAAT 
AAGTACCTGG GGGATGTGAA CAATGTCAAG GTTGTGTATG GACCTGCTGC TCGATTGAGA 
CCCACCGAGG TTCCGGAGAC ATACTATTCG TTTAACTATG AAGCCCTTGC AAAAAATCTT 
TCTTGCCGGG AAACAAACCA GCACTTCCGG CCTTATCTGA AACACTTCTT ACCCAAGCGC 
TTACACTTTG CTAAAAATGA CAGGATTGAG CCACTGACCT TCTATCTGGA CCCTCAATGG 
CAACTTGCCT TGAATCCGTC AGAAAGGAAA TATTGTGGAA GTGGATTTCA TGGCTCTGAC 
AACCTGTTTT CAAACATGCA AGCTCTCTTC ATTGGCTATG GACCTGCCTT CAAGCACGGT 
GCTGAGGTTG ACTCCTTTGA AAATATCGAG GTCTATAACT TAATGTGTGA TTTATTGGGT 
TTGATCCCAG CTCCTAATAA TGGAAGTCAC GGCAGTCTCA ACCATCTTCT AAAGAAACCC 
ATTTATACCC CAAGTCATCC CAAAGAAGAG AGCTTCCTGT CCCAGTGTCC AATCAAATCA 
GTGTCCAGTG ACCTCGGCTG CACATGTGAC CCCTCGATTG TGCCGATCAT GGACTTCGAG 
AAGCAGTTCA ATCTGACCAC GGATGCTGAG GATGTTTACT CTATGACTGT GCCCAACGGG 
AGGCCCCGGA ATCTGCAGAA GCAGCACCGC GTCTGTCTGC TCCATCAGCA ACAGTTTTTG 
ACTGGGTACA GCCTGGACCT CCTCATGCCC CTGTGGACGT CTTACACCTT TCTCAGTAAT 
GACCAGTTCT CCACAGATGA CTTTTCCAAC TGTTTGTACC AGGACCTTCG GATTCCCCTT 
AGTCCCATGC ATAAATGTTC CTATTATAAA AGCACTTCTA AGCTCAGTTA TGGGTTCCTT 
ACACCGCCAA GACTAAACAG AGTCTCACGT CAAATATATT CTGAAGCCTT GCTTACTTCT 
AACATAGTGC CAATGTACCA GAGTTTTCAA GTTATATGGC AATACCTTCA TGACACCGTA 
CTGCGAAGGT ATGCCCAAGA AAGGAATGGC GTCAATGTTG TCAGCGGCCC CGTGTTCGAC 
TTTGATTATG ATGGACGGTA TGATTCCTCA GAGATCCTGA AACAAAACAC CAGAGTCATC 
CGCAGTCAAG AAAATCTGAT TCCCACTCAC TTCTTCATTG TGCTTACCAG CTGCAAGCAG 
CTGTCTGAGA GCCCCTTAAA GTGTACAGCC TTAGAGTCTT CAGCCTTCCT CTTGCCTCAC 
AGGCCTGATA ACATCGAGAG CTGTACACAT GGAAAGCAGG AGTCTGCATG GGTTGAAGAG 
TTGCTGGCAT TGCACAGAGC TCGGGTCACA GACGTTGAGC TCATCACGGG TCTCAGCTTC 
TACCAGGACC GACAGGAGTC AGTTTCAGAA CTGCTGAGGT TGAAAACACA CTTACCAATC 
TTCAGCCAAG AAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006227754.1
CDS	36..2753
Translation	MERDGEQAGQGPRHGPAGNGRELESPAAASLLAPMDLGEEPLEKAERARTAKDPNTYKVLSLVLSVCVLTTILGCIFGLKPSCAKEVKSCKGRCFERTFSNCRCDAACVSLGNCCLDFQETCVEPTHIWTCNKFRCGEKRLSRFVCSCADDCKAHNDCCINYSSVCQEKKSWVEEACETIDAPQCPAEFESPPTLLFSLDGFRAEYLHTWGGLLPVISKLKNCGTYTKNMRPVYPTKTFPNHYSIVTGLYPESHGIIDNKMYDPKMNASFSLKSKEKFNPLWYKGQPIWVTANHQEVRSGTYFWPGSDVEIDGILPDIYKVYNGSVPFEERILAVLEWLQLPSYERPHFYTLYLEEPDSSGHSHGPVSSEVIKALQKVDHIVGMLMDGLKDLGLDKCLNLILISDHGMEQGSCKKYVYLNKYLGDVNNVKVVYGPAARLRPTEVPETYYSFNYEALAKNLSCRETNQHFRPYLKHFLPKRLHFAKNDRIEPLTFYLDPQWQLALNPSERKYCGSGFHGSDNLFSNMQALFIGYGPAFKHGAEVDSFENIEVYNLMCDLLGLIPAPNNGSHGSLNHLLKKPIYTPSHPKEESFLSQCPIKSVSSDLGCTCDPSIVPIMDFEKQFNLTTDAEDVYSMTVPNGRPRNLQKQHRVCLLHQQQFLTGYSLDLLMPLWTSYTFLSNDQFSTDDFSNCLYQDLRIPLSPMHKCSYYKSTSKLSYGFLTPPRLNRVSRQIYSEALLTSNIVPMYQSFQVIWQYLHDTVLRRYAQERNGVNVVSGPVFDFDYDGRYDSSEILKQNTRVIRSQENLIPTHFFIVLTSCKQLSESPLKCTALESSAFLLPHRPDNIESCTHGKQESAWVEELLALHRARVTDVELITGLSFYQDRQESVSELLRLKTHLPIFSQED

Target ORF information:

RefSeq Version	XM_006227692.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ectonucleotide pyrophosphatase/phosphodiesterase 1 (Enpp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006227692.3

ORF Insert Sequence:

ATGGAGCGCG ACGGCGAACA GGCAGGGCAA GGGCCCCGGC ATGGGCCAGC GGGAAACGGC 
CGCGAGCTGG AGTCTCCAGC CGCCGCTTCG CTGCTGGCGC CCATGGACCT AGGGGAGGAG 
CCGCTGGAGA AGGCGGAGCG TGCGCGCACC GCCAAAGACC CCAACACCTA CAAAGTGCTG 
TCGCTGGTTT TGTCAGTATG CGTGCTAACA ACTATTCTTG GTTGTATCTT TGGGTTGAAA 
CCAAGCTGTG CCAAAGAAGT GAAAAGTTGC AAAGGCCGCT GCTTTGAAAG GACGTTCAGC 
AATTGTCGCT GCGATGCTGC CTGTGTCAGC CTTGGGAACT GCTGTCTGGA TTTCCAAGAG 
ACCTGTGTGG AACCAACACA TATATGGACT TGCAACAAGT TCAGGTGCGG CGAGAAGAGG 
CTGTCCAGAT TTGTGTGCTC CTGTGCGGAC GACTGCAAAG CCCACAATGA CTGTTGCATC 
AACTACAGTT CAGTGTGCCA AGAAAAGAAG AGTTGGGTAG AAGAAGCCTG CGAAACCATC 
GATGCACCAC AGTGTCCAGC AGAGTTTGAA TCACCCCCTA CTCTCTTGTT TTCTTTGGAT 
GGATTCAGAG CTGAATATTT GCACACTTGG GGTGGACTTC TTCCTGTCAT TAGCAAGCTG 
AAAAACTGTG GAACGTACAC TAAAAACATG AGGCCTGTGT ACCCTACCAA GACGTTTCCC 
AATCATTACA GCATCGTCAC AGGACTTTAT CCAGAATCAC ATGGCATAAT TGACAACAAG 
ATGTATGATC CTAAAATGAA CGCTTCTTTC TCACTTAAAA GTAAAGAGAA ATTCAACCCT 
CTGTGGTACA AAGGACAGCC GATCTGGGTG ACCGCTAATC ATCAGGAGGT CAGGTCTGGC 
ACATACTTCT GGCCAGGATC AGACGTGGAA ATTGATGGGA TTCTGCCAGA TATCTACAAA 
GTGTATAATG GGTCAGTACC ATTTGAAGAA AGGATTTTAG CTGTTCTCGA GTGGCTACAG 
CTTCCTAGCT ATGAAAGACC ACACTTTTAC ACTCTGTATT TAGAAGAACC AGATTCTTCA 
GGGCATTCAC ACGGACCAGT AAGCAGTGAG GTCATCAAGG CCCTGCAGAA GGTTGACCAC 
ATAGTTGGCA TGCTGATGGA TGGCCTGAAG GACCTGGGCT TGGATAAATG CCTGAACCTC 
ATTCTCATTT CAGATCACGG CATGGAACAA GGCAGTTGTA AGAAGTACGT GTACCTGAAT 
AAGTACCTGG GGGATGTGAA CAATGTCAAG GTTGTGTATG GACCTGCTGC TCGATTGAGA 
CCCACCGAGG TTCCGGAGAC ATACTATTCG TTTAACTATG AAGCCCTTGC AAAAAATCTT 
TCTTGCCGGG AAACAAACCA GCACTTCCGG CCTTATCTGA AACACTTCTT ACCCAAGCGC 
TTACACTTTG CTAAAAATGA CAGGATTGAG CCACTGACCT TCTATCTGGA CCCTCAATGG 
CAACTTGCCT TGAATCCGTC AGAAAGGAAA TATTGTGGAA GTGGATTTCA TGGCTCTGAC 
AACCTGTTTT CAAACATGCA AGCTCTCTTC ATTGGCTATG GACCTGCCTT CAAGCACGGT 
GCTGAGGTTG ACTCCTTTGA AAATATCGAG GTCTATAACT TAATGTGTGA TTTATTGGGT 
TTGATCCCAG CTCCTAATAA TGGAAGTCAC GGCAGTCTCA ACCATCTTCT AAAGAAACCC 
ATTTATACCC CAAGTCATCC CAAAGAAGAG AGCTTCCTGT CCCAGTGTCC AATCAAATCA 
GTGTCCAGTG ACCTCGGCTG CACATGTGAC CCCTCGATTG TGCCGATCAT GGACTTCGAG 
AAGCAGTTCA ATCTGACCAC GGATGCTGAG GATGTTTACT CTATGACTGT GCCCAACGGG 
AGGCCCCGGA ATCTGCAGAA GCAGCACCGC GTCTGTCTGC TCCATCAGCA ACAGTTTTTG 
ACTGGGTACA GCCTGGACCT CCTCATGCCC CTGTGGACGT CTTACACCTT TCTCAGTAAT 
GACCAGTTCT CCACAGATGA CTTTTCCAAC TGTTTGTACC AGGACCTTCG GATTCCCCTT 
AGTCCCATGC ATAAATGTTC CTATTATAAA AGCACTTCTA AGCTCAGTTA TGGGTTCCTT 
ACACCGCCAA GACTAAACAG AGTCTCACGT CAAATATATT CTGAAGCCTT GCTTACTTCT 
AACATAGTGC CAATGTACCA GAGTTTTCAA GTTATATGGC AATACCTTCA TGACACCGTA 
CTGCGAAGGT ATGCCCAAGA AAGGAATGGC GTCAATGTTG TCAGCGGCCC CGTGTTCGAC 
TTTGATTATG ATGGACGGTA TGATTCCTCA GAGATCCTGA AACAAAACAC CAGAGTCATC 
CGCAGTCAAG AAAATCTGAT TCCCACTCAC TTCTTCATTG TGCTTACCAG CTGCAAGCAG 
CTGTCTGAGA GCCCCTTAAA GTGTACAGCC TTAGAGTCTT CAGCCTTCCT CTTGCCTCAC 
AGGCCTGATA ACATCGAGAG CTGTACACAT GGAAAGCAGG AGTCTGCATG GGTTGAAGAG 
TTGCTGGCAT TGCACAGAGC TCGGGTCACA GACGTTGAGC TCATCACGGG TCTCAGCTTC 
TACCAGGACC GACAGGAGTC AGTTTCAGAA CTGCTGAGGT TGAAAACACA CTTACCAATC 
TTCAGCCAAG AAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ENPP1 Mutation Causes Recessive Cole Disease by Altering Melanogenesis.
The Journal of investigative dermatology(2017 Sep)
Chourabi M,Liew MS,Lim S,H'mida-Ben Brahim D,Boussofara L,Dai L,Wong PM,Foo JN,Sriha B,Robinson KS,Denil S,Common JE,Mama? O,Ben Khalifa Y,Bollen M,Liu J,Denguezli M,Bonnard C,Saad A,Reversade B

Effects of Different Variants in the ENPP1 Gene on the Functional Properties of Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 1.
Human mutation37(11)1190-1201(2016 11)
Stella J,Buers I,van de Wetering K,H?hne W,Rutsch F,Nitschke Y

Unilateral anterior crossbite induces aberrant mineral deposition in degenerative temporomandibular cartilage in rats.
Osteoarthritis and cartilage24(5)921-31(2016 May)
Zhang M,Wang H,Zhang J,Zhang H,Yang H,Wan X,Jing L,Lu L,Liu X,Yu S,Chang W,Wang M

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Enpp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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