Pnoc cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pnoc
Entrez Gene ID	18155
Full Name	prepronociceptin
Synonyms	N/OFQ,Npnc1,OFQ/N
General protein information	Preferred Names prepronociceptin Names prepronociceptin neuropeptide nociceptin 1 orphanin FQ proorphanin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 14 Map Location: 14\|14 D1
Summary	This gene encodes the precursor for neuropeptides that have been implicated in a wide range of physiological roles such as transmission and sensitivity to pain, learning, memory, anxiety and depression, in the central nervous system. The encoded protein is a precursor that is proteolytically processed to generate multiple biologically active peptides including nociceptin and nocistatin which have opposite functions in pain transmission. Mice lacking the encoded protein display increased anxiety, elevated basal pain threshold and impaired adaptation to repeated stress. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2015].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006518684.2	XP_006518747.1	prepronociceptin isoform X1
NM_010932.2	NP_035062.1	prepronociceptin isoform 1 preproprotein
XM_006518684.3	XP_006518747.1	prepronociceptin isoform X1
XM_030247683.1	XP_030103543.1	prepronociceptin isoform X2
NM_001205075.1	NP_001192004.1	prepronociceptin isoform 2 preproprotein

Pathways from BioSystems

Homologies

Homo sapiens (human)	PNOC	NP_006219.1
Canis lupus familiaris (dog)	PNOC	XP_005635726.1
Bos taurus (cattle)	PNOC	NP_776575.1
Mus musculus (house mouse)	Pnoc	NP_001192004.1
Rattus norvegicus (Norway rat)	Pnoc	NP_037139.1
Pan troglodytes (chimpanzee)	PNOC	XP_519684.3
Xenopus tropicalis (tropical clawed frog)	pnoc	NP_001106534.1
	PNOC	XP_001110309.1
Danio rerio (zebrafish)	pnoca	XP_003200377.2

Gene Ontology
Bibliography

Related articles in PubMed

Intrathecal administration of low-dose nociceptin/orphanin FQ induces allodynia via c-Jun N-terminal kinase and monocyte chemoattractant protein-1.
Kawabata K, Nishimura I, Fujiwara T, Terauchi S, Minami T, Ito S, Okuda-Ashitaka E
The European journal of neuroscience43(11)1499-508(2016 06)

Nociceptin/orphanin FQ metabolism: role of aminopeptidase and endopeptidase 24.15.
Montiel JL, Cornille F, Roques BP, Noble F
Journal of neurochemistry68(1)354-61(1997 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study demonstrates that nociceptin/orphanin FQ (N/OFQ) activated JNK through the PLC-IP3 signaling pathway, which is coupled to Gi/o, in astrocytes of the spinal dorsal horn. The study also demonstrated that N/OFQ induced monocyte chemoattractant protein-1 release from astrocytes of the spinal cord via JNK activation.
Title: Intrathecal administration of low-dose nociceptin/orphanin FQ induces allodynia via c-Jun N-terminal kinase and monocyte chemoattractant protein-1.

the present results suggest that the endogenous OFQ/N/NOP receptor system may modulate the development of cocaine-induced locomotor sensitization.
Title: The role of NOP receptors in psychomotor stimulation and locomotor sensitization induced by cocaine and amphetamine in mice.

these findings may identify nociceptin as a novel paracrine mediator of the FSH effects in the regulation of spermatogenesis.
Title: Nociceptin is upregulated by FSH signaling in Sertoli cells in murine testes.

Data suggest that nociceptin/nociceptin receptor signaling plays a role in the disease pathogenesis of inflammatory bowel diseases.
Title: Nociceptin/orphanin FQ inhibition with SB612111 ameliorates dextran sodium sulfate-induced colitis.

Endogenous nociceptin/orphanin FQ contributes to reserpine-induced parkinsonism.
Title: Further evidence for an involvement of nociceptin/orphanin FQ in the pathophysiology of Parkinson's disease: a behavioral and neurochemical study in reserpinized mice.

The following Pnoc gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pnoc cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu22719	NM_001205075.1 Latest version!	Mus musculus prepronociceptin (Pnoc), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu22755	NM_010932.2 Latest version!	Mus musculus prepronociceptin (Pnoc), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu22719	XM_006518684.3 Latest version!	Mus musculus prepronociceptin (Pnoc), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu22755	XM_030247683.1 Latest version!	Mus musculus prepronociceptin (Pnoc), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu22719	XM_006518684.2	Mus musculus prepronociceptin (Pnoc), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pnoc ( NM_001205075.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001192004.1 Mus musculus prepronociceptin (Pnoc), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu22719
Clone ID Related Accession (Same CDS sequence)	XM_006518684.2 , XM_006518684.3 , NM_001205075.1
Accession Version	NM_001205075.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Mus musculus(house mouse)
Product	prepronociceptin isoform 2 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC158983.2 and AK045893.1. Transcript Variant: This variant (2) uses an alternate donor splice site at the penultimate exon compared to variant 1. This results in a frameshift, and a longer isoform (2, also known as N27K) with a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC144865.1, D50055.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849376 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAATCC TCTTTTGTGA CGTTCTGCTG CTCAGCCTGC TCTCCAGCGT GTTCAGCAGC 
TGTCCCAGGG ACTGCCTCAC CTGCCAGGAG AAGCTCCACC CAGCTCCAGA CAGCTTCAAC 
TTAAAGACGT GCATCCTCCA GTGTGAAGAG AAGGTCTTCC CCCGCCCTCT CTGGACTGTA 
TGCACCAAAG TCATGGCCAG TGGCTCCGGG CAGCTCAGCC CTGCTGACCC AGAGCTTGTG 
TCAGCTGCTC TTTACCAGCC AAAGGCCTCG GAGATGCAGC ACCTGAAGAG AATGCCGCGT 
GTCCGGAGCT TGGTGCAAGT GCGAGATGCA GAGCCTGGCG CAGATGCTGA GCCTGGCGCA 
GATGCTGAGC CTGGCGCAGA TGACGCTGAG GAGGTGGAGC AGAAGCAGCT GCAGAAAAGG 
TTTGGGGGCT TCACCGGGGC CCGGAAATCA GCCCGGAAGT TGGCCAACCA GAAGCGGTTC 
AGTGAGTTTA TGAGGCAGTA CCTGGTCCTG AGCATGCAGT CAAGTCAACG CCGGCGCACC 
CTGCACCAGA ATGGCATCCA GGTGATCCCC CGTACAGCAT GTGTCCACTC CAAGACCTGC 
AGGCCGGGAG TCAGGATTCC TCCCTCCCCG AGGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192004.1
CDS	360..998
Translation	MKILFCDVLLLSLLSSVFSSCPRDCLTCQEKLHPAPDSFNLKTCILQCEEKVFPRPLWTVCTKVMASGSGQLSPADPELVSAALYQPKASEMQHLKRMPRVRSLVQVRDAEPGADAEPGADAEPGADDAEEVEQKQLQKRFGGFTGARKSARKLANQKRFSEFMRQYLVLSMQSSQRRRTLHQNGIQVIPRTACVHSKTCRPGVRIPPSPRH

Target ORF information:

RefSeq Version	NM_001205075.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus prepronociceptin (Pnoc), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205075.1

ORF Insert Sequence:

ATGAAAATCC TCTTTTGTGA CGTTCTGCTG CTCAGCCTGC TCTCCAGCGT GTTCAGCAGC 
TGTCCCAGGG ACTGCCTCAC CTGCCAGGAG AAGCTCCACC CAGCTCCAGA CAGCTTCAAC 
TTAAAGACGT GCATCCTCCA GTGTGAAGAG AAGGTCTTCC CCCGCCCTCT CTGGACTGTA 
TGCACCAAAG TCATGGCCAG TGGCTCCGGG CAGCTCAGCC CTGCTGACCC AGAGCTTGTG 
TCAGCTGCTC TTTACCAGCC AAAGGCCTCG GAGATGCAGC ACCTGAAGAG AATGCCGCGT 
GTCCGGAGCT TGGTGCAAGT GCGAGATGCA GAGCCTGGCG CAGATGCTGA GCCTGGCGCA 
GATGCTGAGC CTGGCGCAGA TGACGCTGAG GAGGTGGAGC AGAAGCAGCT GCAGAAAAGG 
TTTGGGGGCT TCACCGGGGC CCGGAAATCA GCCCGGAAGT TGGCCAACCA GAAGCGGTTC 
AGTGAGTTTA TGAGGCAGTA CCTGGTCCTG AGCATGCAGT CAAGTCAACG CCGGCGCACC 
CTGCACCAGA ATGGCATCCA GGTGATCCCC CGTACAGCAT GTGTCCACTC CAAGACCTGC 
AGGCCGGGAG TCAGGATTCC TCCCTCCCCG AGGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnoc ( NM_010932.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_035062.1 Mus musculus prepronociceptin (Pnoc), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu22755
Clone ID Related Accession (Same CDS sequence)	NM_010932.2 , XM_030247683.1
Accession Version	NM_010932.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	prepronociceptin isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC158983.2 and AK045893.1. On Mar 22, 2011 this sequence version replaced NM_010932.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the shorter protein (isoform 1, also known as N23K). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK045893.1, BC119372.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAATCC TCTTTTGTGA CGTTCTGCTG CTCAGCCTGC TCTCCAGCGT GTTCAGCAGC 
TGTCCCAGGG ACTGCCTCAC CTGCCAGGAG AAGCTCCACC CAGCTCCAGA CAGCTTCAAC 
TTAAAGACGT GCATCCTCCA GTGTGAAGAG AAGGTCTTCC CCCGCCCTCT CTGGACTGTA 
TGCACCAAAG TCATGGCCAG TGGCTCCGGG CAGCTCAGCC CTGCTGACCC AGAGCTTGTG 
TCAGCTGCTC TTTACCAGCC AAAGGCCTCG GAGATGCAGC ACCTGAAGAG AATGCCGCGT 
GTCCGGAGCT TGGTGCAAGT GCGAGATGCA GAGCCTGGCG CAGATGCTGA GCCTGGCGCA 
GATGCTGAGC CTGGCGCAGA TGACGCTGAG GAGGTGGAGC AGAAGCAGCT GCAGAAAAGG 
TTTGGGGGCT TCACCGGGGC CCGGAAATCA GCCCGGAAGT TGGCCAACCA GAAGCGGTTC 
AGTGAGTTTA TGAGGCAGTA CCTGGTCCTG AGCATGCAGT CAAGTCAACG CCGGCGCACC 
CTGCACCAGA ATGGTAATGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_035062.1
CDS	360..923
Translation	MKILFCDVLLLSLLSSVFSSCPRDCLTCQEKLHPAPDSFNLKTCILQCEEKVFPRPLWTVCTKVMASGSGQLSPADPELVSAALYQPKASEMQHLKRMPRVRSLVQVRDAEPGADAEPGADAEPGADDAEEVEQKQLQKRFGGFTGARKSARKLANQKRFSEFMRQYLVLSMQSSQRRRTLHQNGNV

Target ORF information:

RefSeq Version	NM_010932.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus prepronociceptin (Pnoc), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010932.2

ORF Insert Sequence:

ATGAAAATCC TCTTTTGTGA CGTTCTGCTG CTCAGCCTGC TCTCCAGCGT GTTCAGCAGC 
TGTCCCAGGG ACTGCCTCAC CTGCCAGGAG AAGCTCCACC CAGCTCCAGA CAGCTTCAAC 
TTAAAGACGT GCATCCTCCA GTGTGAAGAG AAGGTCTTCC CCCGCCCTCT CTGGACTGTA 
TGCACCAAAG TCATGGCCAG TGGCTCCGGG CAGCTCAGCC CTGCTGACCC AGAGCTTGTG 
TCAGCTGCTC TTTACCAGCC AAAGGCCTCG GAGATGCAGC ACCTGAAGAG AATGCCGCGT 
GTCCGGAGCT TGGTGCAAGT GCGAGATGCA GAGCCTGGCG CAGATGCTGA GCCTGGCGCA 
GATGCTGAGC CTGGCGCAGA TGACGCTGAG GAGGTGGAGC AGAAGCAGCT GCAGAAAAGG 
TTTGGGGGCT TCACCGGGGC CCGGAAATCA GCCCGGAAGT TGGCCAACCA GAAGCGGTTC 
AGTGAGTTTA TGAGGCAGTA CCTGGTCCTG AGCATGCAGT CAAGTCAACG CCGGCGCACC 
CTGCACCAGA ATGGTAATGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnoc ( XM_006518684.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006518747.1 Mus musculus prepronociceptin (Pnoc), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu22719
Clone ID Related Accession (Same CDS sequence)	XM_006518684.2 , XM_006518684.3 , NM_001205075.1
Accession Version	XM_006518684.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	prepronociceptin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006518684.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATCC TCTTTTGTGA CGTTCTGCTG CTCAGCCTGC TCTCCAGCGT GTTCAGCAGC 
TGTCCCAGGG ACTGCCTCAC CTGCCAGGAG AAGCTCCACC CAGCTCCAGA CAGCTTCAAC 
TTAAAGACGT GCATCCTCCA GTGTGAAGAG AAGGTCTTCC CCCGCCCTCT CTGGACTGTA 
TGCACCAAAG TCATGGCCAG TGGCTCCGGG CAGCTCAGCC CTGCTGACCC AGAGCTTGTG 
TCAGCTGCTC TTTACCAGCC AAAGGCCTCG GAGATGCAGC ACCTGAAGAG AATGCCGCGT 
GTCCGGAGCT TGGTGCAAGT GCGAGATGCA GAGCCTGGCG CAGATGCTGA GCCTGGCGCA 
GATGCTGAGC CTGGCGCAGA TGACGCTGAG GAGGTGGAGC AGAAGCAGCT GCAGAAAAGG 
TTTGGGGGCT TCACCGGGGC CCGGAAATCA GCCCGGAAGT TGGCCAACCA GAAGCGGTTC 
AGTGAGTTTA TGAGGCAGTA CCTGGTCCTG AGCATGCAGT CAAGTCAACG CCGGCGCACC 
CTGCACCAGA ATGGCATCCA GGTGATCCCC CGTACAGCAT GTGTCCACTC CAAGACCTGC 
AGGCCGGGAG TCAGGATTCC TCCCTCCCCG AGGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006518747.1
CDS	211..849
Translation	MKILFCDVLLLSLLSSVFSSCPRDCLTCQEKLHPAPDSFNLKTCILQCEEKVFPRPLWTVCTKVMASGSGQLSPADPELVSAALYQPKASEMQHLKRMPRVRSLVQVRDAEPGADAEPGADAEPGADDAEEVEQKQLQKRFGGFTGARKSARKLANQKRFSEFMRQYLVLSMQSSQRRRTLHQNGIQVIPRTACVHSKTCRPGVRIPPSPRH

Target ORF information:

RefSeq Version	XM_006518684.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus prepronociceptin (Pnoc), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006518684.2

ORF Insert Sequence:

ATGAAAATCC TCTTTTGTGA CGTTCTGCTG CTCAGCCTGC TCTCCAGCGT GTTCAGCAGC 
TGTCCCAGGG ACTGCCTCAC CTGCCAGGAG AAGCTCCACC CAGCTCCAGA CAGCTTCAAC 
TTAAAGACGT GCATCCTCCA GTGTGAAGAG AAGGTCTTCC CCCGCCCTCT CTGGACTGTA 
TGCACCAAAG TCATGGCCAG TGGCTCCGGG CAGCTCAGCC CTGCTGACCC AGAGCTTGTG 
TCAGCTGCTC TTTACCAGCC AAAGGCCTCG GAGATGCAGC ACCTGAAGAG AATGCCGCGT 
GTCCGGAGCT TGGTGCAAGT GCGAGATGCA GAGCCTGGCG CAGATGCTGA GCCTGGCGCA 
GATGCTGAGC CTGGCGCAGA TGACGCTGAG GAGGTGGAGC AGAAGCAGCT GCAGAAAAGG 
TTTGGGGGCT TCACCGGGGC CCGGAAATCA GCCCGGAAGT TGGCCAACCA GAAGCGGTTC 
AGTGAGTTTA TGAGGCAGTA CCTGGTCCTG AGCATGCAGT CAAGTCAACG CCGGCGCACC 
CTGCACCAGA ATGGCATCCA GGTGATCCCC CGTACAGCAT GTGTCCACTC CAAGACCTGC 
AGGCCGGGAG TCAGGATTCC TCCCTCCCCG AGGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnoc ( XM_006518684.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006518747.1 Mus musculus prepronociceptin (Pnoc), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu22719
Clone ID Related Accession (Same CDS sequence)	XM_006518684.2 , XM_006518684.3 , NM_001205075.1
Accession Version	XM_006518684.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 639bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	prepronociceptin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006518684.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATCC TCTTTTGTGA CGTTCTGCTG CTCAGCCTGC TCTCCAGCGT GTTCAGCAGC 
TGTCCCAGGG ACTGCCTCAC CTGCCAGGAG AAGCTCCACC CAGCTCCAGA CAGCTTCAAC 
TTAAAGACGT GCATCCTCCA GTGTGAAGAG AAGGTCTTCC CCCGCCCTCT CTGGACTGTA 
TGCACCAAAG TCATGGCCAG TGGCTCCGGG CAGCTCAGCC CTGCTGACCC AGAGCTTGTG 
TCAGCTGCTC TTTACCAGCC AAAGGCCTCG GAGATGCAGC ACCTGAAGAG AATGCCGCGT 
GTCCGGAGCT TGGTGCAAGT GCGAGATGCA GAGCCTGGCG CAGATGCTGA GCCTGGCGCA 
GATGCTGAGC CTGGCGCAGA TGACGCTGAG GAGGTGGAGC AGAAGCAGCT GCAGAAAAGG 
TTTGGGGGCT TCACCGGGGC CCGGAAATCA GCCCGGAAGT TGGCCAACCA GAAGCGGTTC 
AGTGAGTTTA TGAGGCAGTA CCTGGTCCTG AGCATGCAGT CAAGTCAACG CCGGCGCACC 
CTGCACCAGA ATGGCATCCA GGTGATCCCC CGTACAGCAT GTGTCCACTC CAAGACCTGC 
AGGCCGGGAG TCAGGATTCC TCCCTCCCCG AGGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006518747.1
CDS	194..832
Translation	MKILFCDVLLLSLLSSVFSSCPRDCLTCQEKLHPAPDSFNLKTCILQCEEKVFPRPLWTVCTKVMASGSGQLSPADPELVSAALYQPKASEMQHLKRMPRVRSLVQVRDAEPGADAEPGADAEPGADDAEEVEQKQLQKRFGGFTGARKSARKLANQKRFSEFMRQYLVLSMQSSQRRRTLHQNGIQVIPRTACVHSKTCRPGVRIPPSPRH

Target ORF information:

RefSeq Version	XM_006518684.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus prepronociceptin (Pnoc), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006518684.3

ORF Insert Sequence:

ATGAAAATCC TCTTTTGTGA CGTTCTGCTG CTCAGCCTGC TCTCCAGCGT GTTCAGCAGC 
TGTCCCAGGG ACTGCCTCAC CTGCCAGGAG AAGCTCCACC CAGCTCCAGA CAGCTTCAAC 
TTAAAGACGT GCATCCTCCA GTGTGAAGAG AAGGTCTTCC CCCGCCCTCT CTGGACTGTA 
TGCACCAAAG TCATGGCCAG TGGCTCCGGG CAGCTCAGCC CTGCTGACCC AGAGCTTGTG 
TCAGCTGCTC TTTACCAGCC AAAGGCCTCG GAGATGCAGC ACCTGAAGAG AATGCCGCGT 
GTCCGGAGCT TGGTGCAAGT GCGAGATGCA GAGCCTGGCG CAGATGCTGA GCCTGGCGCA 
GATGCTGAGC CTGGCGCAGA TGACGCTGAG GAGGTGGAGC AGAAGCAGCT GCAGAAAAGG 
TTTGGGGGCT TCACCGGGGC CCGGAAATCA GCCCGGAAGT TGGCCAACCA GAAGCGGTTC 
AGTGAGTTTA TGAGGCAGTA CCTGGTCCTG AGCATGCAGT CAAGTCAACG CCGGCGCACC 
CTGCACCAGA ATGGCATCCA GGTGATCCCC CGTACAGCAT GTGTCCACTC CAAGACCTGC 
AGGCCGGGAG TCAGGATTCC TCCCTCCCCG AGGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pnoc ( XM_030247683.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030103543.1 Mus musculus prepronociceptin (Pnoc), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu22755
Clone ID Related Accession (Same CDS sequence)	NM_010932.2 , XM_030247683.1
Accession Version	XM_030247683.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	prepronociceptin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000080.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAATCC TCTTTTGTGA CGTTCTGCTG CTCAGCCTGC TCTCCAGCGT GTTCAGCAGC 
TGTCCCAGGG ACTGCCTCAC CTGCCAGGAG AAGCTCCACC CAGCTCCAGA CAGCTTCAAC 
TTAAAGACGT GCATCCTCCA GTGTGAAGAG AAGGTCTTCC CCCGCCCTCT CTGGACTGTA 
TGCACCAAAG TCATGGCCAG TGGCTCCGGG CAGCTCAGCC CTGCTGACCC AGAGCTTGTG 
TCAGCTGCTC TTTACCAGCC AAAGGCCTCG GAGATGCAGC ACCTGAAGAG AATGCCGCGT 
GTCCGGAGCT TGGTGCAAGT GCGAGATGCA GAGCCTGGCG CAGATGCTGA GCCTGGCGCA 
GATGCTGAGC CTGGCGCAGA TGACGCTGAG GAGGTGGAGC AGAAGCAGCT GCAGAAAAGG 
TTTGGGGGCT TCACCGGGGC CCGGAAATCA GCCCGGAAGT TGGCCAACCA GAAGCGGTTC 
AGTGAGTTTA TGAGGCAGTA CCTGGTCCTG AGCATGCAGT CAAGTCAACG CCGGCGCACC 
CTGCACCAGA ATGGTAATGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030103543.1
CDS	143..706
Translation	MKILFCDVLLLSLLSSVFSSCPRDCLTCQEKLHPAPDSFNLKTCILQCEEKVFPRPLWTVCTKVMASGSGQLSPADPELVSAALYQPKASEMQHLKRMPRVRSLVQVRDAEPGADAEPGADAEPGADDAEEVEQKQLQKRFGGFTGARKSARKLANQKRFSEFMRQYLVLSMQSSQRRRTLHQNGNV

Target ORF information:

RefSeq Version	XM_030247683.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus prepronociceptin (Pnoc), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030247683.1

ORF Insert Sequence:

ATGAAAATCC TCTTTTGTGA CGTTCTGCTG CTCAGCCTGC TCTCCAGCGT GTTCAGCAGC 
TGTCCCAGGG ACTGCCTCAC CTGCCAGGAG AAGCTCCACC CAGCTCCAGA CAGCTTCAAC 
TTAAAGACGT GCATCCTCCA GTGTGAAGAG AAGGTCTTCC CCCGCCCTCT CTGGACTGTA 
TGCACCAAAG TCATGGCCAG TGGCTCCGGG CAGCTCAGCC CTGCTGACCC AGAGCTTGTG 
TCAGCTGCTC TTTACCAGCC AAAGGCCTCG GAGATGCAGC ACCTGAAGAG AATGCCGCGT 
GTCCGGAGCT TGGTGCAAGT GCGAGATGCA GAGCCTGGCG CAGATGCTGA GCCTGGCGCA 
GATGCTGAGC CTGGCGCAGA TGACGCTGAG GAGGTGGAGC AGAAGCAGCT GCAGAAAAGG 
TTTGGGGGCT TCACCGGGGC CCGGAAATCA GCCCGGAAGT TGGCCAACCA GAAGCGGTTC 
AGTGAGTTTA TGAGGCAGTA CCTGGTCCTG AGCATGCAGT CAAGTCAACG CCGGCGCACC 
CTGCACCAGA ATGGTAATGT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Intrathecal administration of low-dose nociceptin/orphanin FQ induces allodynia via c-Jun N-terminal kinase and monocyte chemoattractant protein-1.
The European journal of neuroscience43(11)1499-508(2016 06)
Kawabata K,Nishimura I,Fujiwara T,Terauchi S,Minami T,Ito S,Okuda-Ashitaka E

Nociceptin/orphanin FQ metabolism: role of aminopeptidase and endopeptidase 24.15.
Journal of neurochemistry68(1)354-61(1997 Jan)
Montiel JL,Cornille F,Roques BP,Noble F

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Pnoc cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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