Mpz cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mpz
Entrez Gene ID	17528
Full Name	myelin protein zero
Synonyms	Mpp,P-zero,P0
General protein information	Preferred Names myelin protein zero Names myelin protein P0 myelin peripheral protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 H3\|1 79.05 cM
Summary	This gene is specifically expressed in Schwann cells of the peripheral nervous system and encodes a type I transmembrane glycoprotein that is a major structural protein of the peripheral myelin sheath. The encoded protein contains a large hydrophobic extracellular domain and a smaller basic intracellular domain, which are essential for the formation and stabilization of the multilamellar structure of the compact myelin. Mutations in the orthologous gene in human are associated with myelinating neuropathies. A recent study showed that two isoforms are produced from the same mRNA by use of alternative in-frame translation termination codons via a stop codon readthrough mechanism. Alternatively spliced transcript variants have also been found for this gene. [provided by RefSeq, Oct 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001315500.1	NP_001302429.1	myelin protein P0 isoform L-MPZ precursor
NM_001315499.1	NP_001302428.1	myelin protein P0 isoform MPZ precursor
NM_008623.5	NP_032649.2	myelin protein P0 isoform MPZ precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LOC100859605	XP_003643863.1
Danio rerio (zebrafish)	mpz	NP_919342.1
Canis lupus familiaris (dog)	MPZ	XP_545771.1
Pan troglodytes (chimpanzee)	MPZ	XP_001173975.1
Mus musculus (house mouse)	Mpz	NP_032649.2
Homo sapiens (human)	MPZ	NP_000521.2
	LOC719969	XP_001118030.1
Xenopus tropicalis (tropical clawed frog)	mpz	NP_001072741.1
Bos taurus (cattle)	MPZ	NP_001072975.1
Rattus norvegicus (Norway rat)	Mpz	NP_058723.1

Gene Ontology
Bibliography

Related articles in PubMed

Phylogenetically Conserved Sequences Around Myelin P0 Stop Codon are Essential for Translational Readthrough to Produce L-MPZ.
Yamaguchi Y, Baba H
Neurochemical research43(1)227-237(2018 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that both transgenic Wnt1-cre and P0-cre are similarly effective in deleting beta-catenin in the neural crest.
Title: ?-catenin is required in the neural crest and mesencephalon for pituitary gland organogenesis.

In the absence of P0, thus, PLP also contributes to myelination by Schwann cells and to the preservation of peripheral axons
Title: Proteolipid protein modulates preservation of peripheral axons and premature death when myelin protein zero is lacking.

interaction with neurofascins impaired by mutations D6Y, D32G, and H52Y responsible for late onset forms of the human disease
Title: HDAC1/2-Dependent P0 Expression Maintains Paranodal and Nodal Integrity Independently of Myelin Stability through Interactions with Neurofascins.

while PLP can co-exist with P0 in peripheral nervous system(PNS) myelin, PLP cannot replace P0 as the major structural protein of PNS myelin.
Title: Proteolipid protein cannot replace P0 protein as the major structural protein of peripheral nervous system myelin.

Mpz-induced murine experimental autoimmune neuritis is a CD4-positive T cell-mediated monophasic axonal inflammatory neuropathy.
Title: Costimulatory molecule CD40 is essential for myelin protein 0 peptide 106-125-induced experimental autoimmune neuritis in mice.

The following Mpz gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mpz cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02398	NM_008623.5 Latest version!	Mus musculus myelin protein zero (Mpz), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu02398	NM_001315499.1 Latest version!	Mus musculus myelin protein zero (Mpz), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu76886	NM_001315500.1 Latest version!	Mus musculus myelin protein zero (Mpz), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mpz ( NM_001315499.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001302428.1 Mus musculus myelin protein zero (Mpz), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu02398
Clone ID Related Accession (Same CDS sequence)	NM_001315499.1 , NM_008623.5
Accession Version	NM_001315499.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	myelin protein P0 isoform MPZ precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BB849278.1, BY339244.1, AC163497.14, AK157960.1 and AA920638.1. On Oct 7, 2015 this sequence version replaced XM_006496694.2. Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (MPZ, also known as P0) results from translation termination at the upstream UAG stop codon, while the longer isoform (L-MPZ) results from UAG stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the shorter isoform (MPZ). Variants 1 and 2 encode the same isoform. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC139139.1, AK157960.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCCCG GGGCTCCCTC CTCCAGCCCC AGCCCTATCC TGGCTGCCCT GCTCTTCTCT 
TCTTTGGTGC TCTCTCCAGC CCTGGCCATT GTGGTTTACA CGGACAGGGA AATCTATGGT 
GCCGTGGGCT CCCAGGTGAC CCTGCACTGC TCCTTCTGGT CCAGTGAATG GGTCTCAGAT 
GACATCTCTT TTACCTGGCG CTACCAGCCT GAAGGGGGCC GAGATGCCAT TTCGATCTTC 
CACTATGCCA AGGGACAACC TTACATCGAT GAGGTGGGGA CCTTCAAAGA GCGCATCCAG 
TGGGTAGGGG ACCCTCGCTG GAAGGATGGC TCCATTGTCA TACACAACCT AGACTACAGT 
GACAACGGCA CTTTCACATG TGATGTCAAA AACCCACCGG ACATAGTGGG CAAGACCTCT 
CAGGTCACGC TCTATGTCTT TGAAAAAGTG CCCACTAGGT ATGGGGTGGT GTTGGGAGCA 
GTGATCGGGG GCATCCTCGG GGTGGTGCTG TTGCTGCTGT TGCTCTTCTA CCTGATTCGG 
TACTGCTGGC TGCGCAGGCA GGCTGCCCTG CAGAGAAGGC TCAGTGCCAT GGAGAAGGGG 
AGATTTCACA AATCTTCGAA GGACTCCTCG AAGCGAGGGC GGCAGACGCC AGTGCTGTAT 
GCCATGCTGG ACCACAGCCG AAGCACCAAA GCTGCCAGTG AGAAGAAATC AAAAGGGCTG 
GGGGAGTCTC GCAAGGATAA GAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001302428.1
CDS	65..811
Translation	MAPGAPSSSPSPILAALLFSSLVLSPALAIVVYTDREIYGAVGSQVTLHCSFWSSEWVSDDISFTWRYQPEGGRDAISIFHYAKGQPYIDEVGTFKERIQWVGDPRWKDGSIVIHNLDYSDNGTFTCDVKNPPDIVGKTSQVTLYVFEKVPTRYGVVLGAVIGGILGVVLLLLLLFYLIRYCWLRRQAALQRRLSAMEKGRFHKSSKDSSKRGRQTPVLYAMLDHSRSTKAASEKKSKGLGESRKDKK

Target ORF information:

RefSeq Version	NM_001315499.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myelin protein zero (Mpz), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001315499.1

ORF Insert Sequence:

ATGGCTCCCG GGGCTCCCTC CTCCAGCCCC AGCCCTATCC TGGCTGCCCT GCTCTTCTCT 
TCTTTGGTGC TCTCTCCAGC CCTGGCCATT GTGGTTTACA CGGACAGGGA AATCTATGGT 
GCCGTGGGCT CCCAGGTGAC CCTGCACTGC TCCTTCTGGT CCAGTGAATG GGTCTCAGAT 
GACATCTCTT TTACCTGGCG CTACCAGCCT GAAGGGGGCC GAGATGCCAT TTCGATCTTC 
CACTATGCCA AGGGACAACC TTACATCGAT GAGGTGGGGA CCTTCAAAGA GCGCATCCAG 
TGGGTAGGGG ACCCTCGCTG GAAGGATGGC TCCATTGTCA TACACAACCT AGACTACAGT 
GACAACGGCA CTTTCACATG TGATGTCAAA AACCCACCGG ACATAGTGGG CAAGACCTCT 
CAGGTCACGC TCTATGTCTT TGAAAAAGTG CCCACTAGGT ATGGGGTGGT GTTGGGAGCA 
GTGATCGGGG GCATCCTCGG GGTGGTGCTG TTGCTGCTGT TGCTCTTCTA CCTGATTCGG 
TACTGCTGGC TGCGCAGGCA GGCTGCCCTG CAGAGAAGGC TCAGTGCCAT GGAGAAGGGG 
AGATTTCACA AATCTTCGAA GGACTCCTCG AAGCGAGGGC GGCAGACGCC AGTGCTGTAT 
GCCATGCTGG ACCACAGCCG AAGCACCAAA GCTGCCAGTG AGAAGAAATC AAAAGGGCTG 
GGGGAGTCTC GCAAGGATAA GAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mpz ( NM_008623.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032649.2 Mus musculus myelin protein zero (Mpz), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu02398
Clone ID Related Accession (Same CDS sequence)	NM_001315499.1 , NM_008623.5
Accession Version	NM_008623.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	myelin protein P0 isoform MPZ precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY201877.1, AC163497.14, BY339244.1, AK157960.1 and AA920638.1. On Oct 7, 2015 this sequence version replaced NM_008623.4. Transcript Variant: This variant (2) has additional 5' non-coding exons compared to variant 1. Variants 1 and 2 encode the same isoform (MPZ). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK089180.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN00849384 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCCCG GGGCTCCCTC CTCCAGCCCC AGCCCTATCC TGGCTGCCCT GCTCTTCTCT 
TCTTTGGTGC TCTCTCCAGC CCTGGCCATT GTGGTTTACA CGGACAGGGA AATCTATGGT 
GCCGTGGGCT CCCAGGTGAC CCTGCACTGC TCCTTCTGGT CCAGTGAATG GGTCTCAGAT 
GACATCTCTT TTACCTGGCG CTACCAGCCT GAAGGGGGCC GAGATGCCAT TTCGATCTTC 
CACTATGCCA AGGGACAACC TTACATCGAT GAGGTGGGGA CCTTCAAAGA GCGCATCCAG 
TGGGTAGGGG ACCCTCGCTG GAAGGATGGC TCCATTGTCA TACACAACCT AGACTACAGT 
GACAACGGCA CTTTCACATG TGATGTCAAA AACCCACCGG ACATAGTGGG CAAGACCTCT 
CAGGTCACGC TCTATGTCTT TGAAAAAGTG CCCACTAGGT ATGGGGTGGT GTTGGGAGCA 
GTGATCGGGG GCATCCTCGG GGTGGTGCTG TTGCTGCTGT TGCTCTTCTA CCTGATTCGG 
TACTGCTGGC TGCGCAGGCA GGCTGCCCTG CAGAGAAGGC TCAGTGCCAT GGAGAAGGGG 
AGATTTCACA AATCTTCGAA GGACTCCTCG AAGCGAGGGC GGCAGACGCC AGTGCTGTAT 
GCCATGCTGG ACCACAGCCG AAGCACCAAA GCTGCCAGTG AGAAGAAATC AAAAGGGCTG 
GGGGAGTCTC GCAAGGATAA GAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032649.2
CDS	167..913
Translation	MAPGAPSSSPSPILAALLFSSLVLSPALAIVVYTDREIYGAVGSQVTLHCSFWSSEWVSDDISFTWRYQPEGGRDAISIFHYAKGQPYIDEVGTFKERIQWVGDPRWKDGSIVIHNLDYSDNGTFTCDVKNPPDIVGKTSQVTLYVFEKVPTRYGVVLGAVIGGILGVVLLLLLLFYLIRYCWLRRQAALQRRLSAMEKGRFHKSSKDSSKRGRQTPVLYAMLDHSRSTKAASEKKSKGLGESRKDKK

Target ORF information:

RefSeq Version	NM_008623.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus myelin protein zero (Mpz), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008623.5

ORF Insert Sequence:

ATGGCTCCCG GGGCTCCCTC CTCCAGCCCC AGCCCTATCC TGGCTGCCCT GCTCTTCTCT 
TCTTTGGTGC TCTCTCCAGC CCTGGCCATT GTGGTTTACA CGGACAGGGA AATCTATGGT 
GCCGTGGGCT CCCAGGTGAC CCTGCACTGC TCCTTCTGGT CCAGTGAATG GGTCTCAGAT 
GACATCTCTT TTACCTGGCG CTACCAGCCT GAAGGGGGCC GAGATGCCAT TTCGATCTTC 
CACTATGCCA AGGGACAACC TTACATCGAT GAGGTGGGGA CCTTCAAAGA GCGCATCCAG 
TGGGTAGGGG ACCCTCGCTG GAAGGATGGC TCCATTGTCA TACACAACCT AGACTACAGT 
GACAACGGCA CTTTCACATG TGATGTCAAA AACCCACCGG ACATAGTGGG CAAGACCTCT 
CAGGTCACGC TCTATGTCTT TGAAAAAGTG CCCACTAGGT ATGGGGTGGT GTTGGGAGCA 
GTGATCGGGG GCATCCTCGG GGTGGTGCTG TTGCTGCTGT TGCTCTTCTA CCTGATTCGG 
TACTGCTGGC TGCGCAGGCA GGCTGCCCTG CAGAGAAGGC TCAGTGCCAT GGAGAAGGGG 
AGATTTCACA AATCTTCGAA GGACTCCTCG AAGCGAGGGC GGCAGACGCC AGTGCTGTAT 
GCCATGCTGG ACCACAGCCG AAGCACCAAA GCTGCCAGTG AGAAGAAATC AAAAGGGCTG 
GGGGAGTCTC GCAAGGATAA GAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mpz ( NM_001315500.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001302429.1 Mus musculus myelin protein zero (Mpz), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu76886
Clone ID Related Accession (Same CDS sequence)	NM_001315500.1
Accession Version	NM_001315500.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-26
Organism	Mus musculus(house mouse)
Product	myelin protein P0 isoform L-MPZ precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BB849278.1, BY339244.1, AC163497.14, AK157960.1 and AA920638.1. Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (MPZ, also known as P0) results from translation termination at the upstream UAG stop codon, while the longer isoform (L-MPZ) results from UAG stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the longer, C-terminally extended isoform (L-MPZ). As the UAG stop codon has been reported to specify several alternative amino acids (glutamine, tyrosine and leucine), its location in the longer isoform is denoted by an 'X'. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC139139.1, AK157960.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## stop codon readthrough :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTCCCG GGGCTCCCTC CTCCAGCCCC AGCCCTATCC TGGCTGCCCT GCTCTTCTCT 
TCTTTGGTGC TCTCTCCAGC CCTGGCCATT GTGGTTTACA CGGACAGGGA AATCTATGGT 
GCCGTGGGCT CCCAGGTGAC CCTGCACTGC TCCTTCTGGT CCAGTGAATG GGTCTCAGAT 
GACATCTCTT TTACCTGGCG CTACCAGCCT GAAGGGGGCC GAGATGCCAT TTCGATCTTC 
CACTATGCCA AGGGACAACC TTACATCGAT GAGGTGGGGA CCTTCAAAGA GCGCATCCAG 
TGGGTAGGGG ACCCTCGCTG GAAGGATGGC TCCATTGTCA TACACAACCT AGACTACAGT 
GACAACGGCA CTTTCACATG TGATGTCAAA AACCCACCGG ACATAGTGGG CAAGACCTCT 
CAGGTCACGC TCTATGTCTT TGAAAAAGTG CCCACTAGGT ATGGGGTGGT GTTGGGAGCA 
GTGATCGGGG GCATCCTCGG GGTGGTGCTG TTGCTGCTGT TGCTCTTCTA CCTGATTCGG 
TACTGCTGGC TGCGCAGGCA GGCTGCCCTG CAGAGAAGGC TCAGTGCCAT GGAGAAGGGG 
AGATTTCACA AATCTTCGAA GGACTCCTCG AAGCGAGGGC GGCAGACGCC AGTGCTGTAT 
GCCATGCTGG ACCACAGCCG AAGCACCAAA GCTGCCAGTG AGAAGAAATC AAAAGGGCTG 
GGGGAGTCTC GCAAGGATAA GAAATAGCGG TTAGCGGGCC GGGCGGGGGG TCGGGGGTCT 
GCGACGGAGT CCTCCAAAGG CTCTCAGGTG GTGGTCATCG AGATGGAGCT TCGCAAAGAT 
GAGCAGAGCT CGGAGCTCCG GCCTGCAGTC AAGTCCCCCA GTAGAACCAG CCTCAAGAAC 
GCCCTCAAGA ACATGATGGG CCTGGACTCG GACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001302429.1
CDS	65..1003
Translation	MAPGAPSSSPSPILAALLFSSLVLSPALAIVVYTDREIYGAVGSQVTLHCSFWSSEWVSDDISFTWRYQPEGGRDAISIFHYAKGQPYIDEVGTFKERIQWVGDPRWKDGSIVIHNLDYSDNGTFTCDVKNPPDIVGKTSQVTLYVFEKVPTRYGVVLGAVIGGILGVVLLLLLLFYLIRYCWLRRQAALQRRLSAMEKGRFHKSSKDSSKRGRQTPVLYAMLDHSRSTKAASEKKSKGLGESRKDKK*RLAGRAGGRGSATESSKGSQVVVIEMELRKDEQSSELRPAVKSPSRTSLKNALKNMMGLDSDK

Target ORF information:

RefSeq Version	NM_001315500.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus myelin protein zero (Mpz), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001315500.1

ORF Insert Sequence:

ATGGCTCCCG GGGCTCCCTC CTCCAGCCCC AGCCCTATCC TGGCTGCCCT GCTCTTCTCT 
TCTTTGGTGC TCTCTCCAGC CCTGGCCATT GTGGTTTACA CGGACAGGGA AATCTATGGT 
GCCGTGGGCT CCCAGGTGAC CCTGCACTGC TCCTTCTGGT CCAGTGAATG GGTCTCAGAT 
GACATCTCTT TTACCTGGCG CTACCAGCCT GAAGGGGGCC GAGATGCCAT TTCGATCTTC 
CACTATGCCA AGGGACAACC TTACATCGAT GAGGTGGGGA CCTTCAAAGA GCGCATCCAG 
TGGGTAGGGG ACCCTCGCTG GAAGGATGGC TCCATTGTCA TACACAACCT AGACTACAGT 
GACAACGGCA CTTTCACATG TGATGTCAAA AACCCACCGG ACATAGTGGG CAAGACCTCT 
CAGGTCACGC TCTATGTCTT TGAAAAAGTG CCCACTAGGT ATGGGGTGGT GTTGGGAGCA 
GTGATCGGGG GCATCCTCGG GGTGGTGCTG TTGCTGCTGT TGCTCTTCTA CCTGATTCGG 
TACTGCTGGC TGCGCAGGCA GGCTGCCCTG CAGAGAAGGC TCAGTGCCAT GGAGAAGGGG 
AGATTTCACA AATCTTCGAA GGACTCCTCG AAGCGAGGGC GGCAGACGCC AGTGCTGTAT 
GCCATGCTGG ACCACAGCCG AAGCACCAAA GCTGCCAGTG AGAAGAAATC AAAAGGGCTG 
GGGGAGTCTC GCAAGGATAA GAAATAGCGG TTAGCGGGCC GGGCGGGGGG TCGGGGGTCT 
GCGACGGAGT CCTCCAAAGG CTCTCAGGTG GTGGTCATCG AGATGGAGCT TCGCAAAGAT 
GAGCAGAGCT CGGAGCTCCG GCCTGCAGTC AAGTCCCCCA GTAGAACCAG CCTCAAGAAC 
GCCCTCAAGA ACATGATGGG CCTGGACTCG GACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phylogenetically Conserved Sequences Around Myelin P0 Stop Codon are Essential for Translational Readthrough to Produce L-MPZ.
Neurochemical research43(1)227-237(2018 Jan)
Yamaguchi Y,Baba H

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Mpz cDNA ORF clone, Mus musculus(house mouse)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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