Mpz cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mpz
Entrez Gene ID	24564
Full Name	myelin protein zero
Synonyms	MPP,P0
General protein information	Preferred Names myelin protein zero Names myelin protein P0 myelin peripheral protein myelin protein zero (Charcot-Marie-Tooth neuropathy 1B)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q24
Summary	This gene is specifically expressed in Schwann cells of the peripheral nervous system and encodes a type I transmembrane glycoprotein that is a major structural protein of the peripheral myelin sheath. The encoded protein contains a large hydrophobic extracellular domain and a smaller basic intracellular domain, which are essential for the formation and stabilization of the multilamellar structure of the compact myelin. Mutations in the orthologous gene in human are associated with myelinating neuropathies. A recent study showed that two isoforms are produced from the same mRNA by use of alternative in-frame translation termination codons via a stop codon readthrough mechanism. [provided by RefSeq, Oct 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001314068.1	NP_001300997.1	myelin protein P0 isoform L-MPZ precursor
NM_017027.2	NP_058723.2	myelin protein P0 isoform MPZ precursor

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	LOC100859605	XP_003643863.1
Danio rerio (zebrafish)	mpz	NP_919342.1
Canis lupus familiaris (dog)	MPZ	XP_545771.1
Pan troglodytes (chimpanzee)	MPZ	XP_001173975.1
Mus musculus (house mouse)	Mpz	NP_032649.2
Homo sapiens (human)	MPZ	NP_000521.2
	LOC719969	XP_001118030.1
Xenopus tropicalis (tropical clawed frog)	mpz	NP_001072741.1
Bos taurus (cattle)	MPZ	NP_001072975.1
Rattus norvegicus (Norway rat)	Mpz	NP_058723.1

Gene Ontology
Bibliography

Related articles in PubMed

Phylogenetically Conserved Sequences Around Myelin P0 Stop Codon are Essential for Translational Readthrough to Produce L-MPZ.
Yamaguchi Y, Baba H
Neurochemical research43(1)227-237(2018 Jan)

Electrical stimulation enhances peripheral nerve regeneration after crush injury in rats.
Zhang X, Xin N, Tong L, Tong XJ
Molecular medicine reports7(5)1523-7(2013 May)

L-MPZ, a novel isoform of myelin P0, is produced by stop codon readthrough.
Yamaguchi Y, Hayashi A, Campagnoni CW, Kimura A, Inuzuka T, Baba H
The Journal of biological chemistry287(21)17765-76(2012 May)

Misreading of termination codons in eukaryotes by natural nonsense suppressor tRNAs.
Beier H, Grimm M
Nucleic acids research29(23)4767-82(2001 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

steady-state levels of insulin receptor mRNA increase during development and after postnatal day 10, when the peak of myelin structural gene (P0) expression occurs, increasing further in parallel with the growth of the myelin sheath
Title: Developmental regulation of insulin receptor gene in sciatic nerves and role of insulin on glycoprotein P0 in the Schwann cells.

L-MPZ, a novel isoform of myelin P0, is produced by stop codon readthrough.
Title: L-MPZ, a novel isoform of myelin P0, is produced by stop codon readthrough.

a rare myelin protein zero (MPZ) variant alters enhancer activity in vitro and in vivo
Title: A rare myelin protein zero (MPZ) variant alters enhancer activity in vitro and in vivo.

These results provided interesting experimental evidence for understanding the mechanism of demyelinating neuropathies caused by MPZ gene malfunction.
Title: Mpz gene suppression by shRNA increases Schwann cell apoptosis in vitro.

The P0 in sciatic nerve of rats could be destroyed by 2,5-hexanedione and P0 expression level decreased with the time of 2,5-hexanedione administration.
Title: [Effect of 2,5-hexanedione on myelin protein zero in sciatic nerve and its antibody in serum of rats at different time points].

The following Mpz gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mpz cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa63218	NM_001314068.1 Latest version!	Rattus norvegicus myelin protein zero (Mpz), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ORa63259	NM_017027.2 Latest version!	Rattus norvegicus myelin protein zero (Mpz), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mpz ( NM_001314068.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001300997.1 Rattus norvegicus myelin protein zero (Mpz), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa63218
Clone ID Related Accession (Same CDS sequence)	NM_001314068.1
Accession Version	NM_001314068.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 939bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Rattus norvegicus(Norway rat)
Product	myelin protein P0 isoform L-MPZ precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB608173.1 and BC078859.1. Transcript Variant: This transcript (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (MPZ, also known as P0) results from translation termination at the upstream UAG stop codon, while the longer isoform (L-MPZ) results from UAG stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the longer, C-terminally extended isoform (L-MPZ). As the UAG stop codon has been reported to specify several alternative amino acids (glutamine, tyrosine and leucine), its location in the longer isoform is denoted by an 'X'. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC078859.1, K03242.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689600, SAMEA2689602 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal stop codon readthrough :: PMID: 22457349, 29081003 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTCCTG GGGCTCCCTC ATCCAGCCCC AGCCCTATCC TGGCTGCCCT GCTCTTCTCT 
TCTTTGGTGC TGTCCCCAAC CCTGGCCATT GTGGTTTACA CGGACAGGGA AGTCTATGGT 
GCTGTGGGCT CCCAGGTGAC CCTGCACTGC TCCTTCTGGT CCAGTGAATG GGTCTCAGAT 
GACATCTCTT TTACCTGGCG CTACCAGCCT GAAGGAGGCC GAGATGCCAT TTCAATCTTC 
CACTATGCCA AGGGTCAACC TTACATCGAT GAGGTGGGGA CCTTCAAGGA GCGCATCCAG 
TGGGTAGGGG ACCCTAGCTG GAAGGATGGC TCCATTGTCA TACACAACCT AGACTACAGT 
GACAACGGCA CTTTCACATG TGATGTCAAA AACCCACCGG ACATAGTGGG CAAGACGTCT 
CAGGTCACGC TCTATGTCTT TGAAAAAGTG CCCACTAGGT ATGGGGTGGT GTTGGGAGCC 
GTGATCGGTG GCATCCTCGG GGTGGTGCTG TTGCTGCTGT TGCTCTTCTA CCTGATCCGG 
TACTGCTGGC TGCGCAGGCA GGCTGCCCTG CAGAGGAGGC TCAGTGCCAT GGAGAAGGGG 
AAATTTCACA AGTCTTCTAA GGACTCCTCG AAGCGCGGGC GGCAGACGCC AGTGCTGTAT 
GCCATGCTGG ACCACAGCCG AAGCACCAAA GCTGCCAGTG AGAAGAAATC TAAAGGGCTG 
GGGGAGTCTC GCAAGGATAA GAAATAGCGG TTAGCGGGCC GGGCGGGGGG TCGGGGGTCT 
GCGATGGAGT CTTCCAAAGG CTCTCAGGTG GTGGTCATCG AGATGGAGCT TCGCAAAGAT 
GAGCAGAGCT CGGAGCTCCG GCCTGCAGTC AAATCCCCCA GTAGAACCAG CCTCAAGAAC 
GCCCTCAAGA ACATGATGGG CCTGGACTCG GACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300997.1
CDS	171..1109
Translation	MAPGAPSSSPSPILAALLFSSLVLSPTLAIVVYTDREVYGAVGSQVTLHCSFWSSEWVSDDISFTWRYQPEGGRDAISIFHYAKGQPYIDEVGTFKERIQWVGDPSWKDGSIVIHNLDYSDNGTFTCDVKNPPDIVGKTSQVTLYVFEKVPTRYGVVLGAVIGGILGVVLLLLLLFYLIRYCWLRRQAALQRRLSAMEKGKFHKSSKDSSKRGRQTPVLYAMLDHSRSTKAASEKKSKGLGESRKDKK*RLAGRAGGRGSAMESSKGSQVVVIEMELRKDEQSSELRPAVKSPSRTSLKNALKNMMGLDSDK

Target ORF information:

RefSeq Version	NM_001314068.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus myelin protein zero (Mpz), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001314068.1

ORF Insert Sequence:

ATGGCTCCTG GGGCTCCCTC ATCCAGCCCC AGCCCTATCC TGGCTGCCCT GCTCTTCTCT 
TCTTTGGTGC TGTCCCCAAC CCTGGCCATT GTGGTTTACA CGGACAGGGA AGTCTATGGT 
GCTGTGGGCT CCCAGGTGAC CCTGCACTGC TCCTTCTGGT CCAGTGAATG GGTCTCAGAT 
GACATCTCTT TTACCTGGCG CTACCAGCCT GAAGGAGGCC GAGATGCCAT TTCAATCTTC 
CACTATGCCA AGGGTCAACC TTACATCGAT GAGGTGGGGA CCTTCAAGGA GCGCATCCAG 
TGGGTAGGGG ACCCTAGCTG GAAGGATGGC TCCATTGTCA TACACAACCT AGACTACAGT 
GACAACGGCA CTTTCACATG TGATGTCAAA AACCCACCGG ACATAGTGGG CAAGACGTCT 
CAGGTCACGC TCTATGTCTT TGAAAAAGTG CCCACTAGGT ATGGGGTGGT GTTGGGAGCC 
GTGATCGGTG GCATCCTCGG GGTGGTGCTG TTGCTGCTGT TGCTCTTCTA CCTGATCCGG 
TACTGCTGGC TGCGCAGGCA GGCTGCCCTG CAGAGGAGGC TCAGTGCCAT GGAGAAGGGG 
AAATTTCACA AGTCTTCTAA GGACTCCTCG AAGCGCGGGC GGCAGACGCC AGTGCTGTAT 
GCCATGCTGG ACCACAGCCG AAGCACCAAA GCTGCCAGTG AGAAGAAATC TAAAGGGCTG 
GGGGAGTCTC GCAAGGATAA GAAATAGCGG TTAGCGGGCC GGGCGGGGGG TCGGGGGTCT 
GCGATGGAGT CTTCCAAAGG CTCTCAGGTG GTGGTCATCG AGATGGAGCT TCGCAAAGAT 
GAGCAGAGCT CGGAGCTCCG GCCTGCAGTC AAATCCCCCA GTAGAACCAG CCTCAAGAAC 
GCCCTCAAGA ACATGATGGG CCTGGACTCG GACAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mpz ( NM_017027.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058723.2 Rattus norvegicus myelin protein zero (Mpz), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ORa63259
Clone ID Related Accession (Same CDS sequence)	NM_017027.2
Accession Version	NM_017027.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Rattus norvegicus(Norway rat)
Product	myelin protein P0 isoform MPZ precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB608173.1 and BC078859.1. On Oct 5, 2015 this sequence version replaced NM_017027.1. Transcript Variant: This transcript (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (MPZ, also known as P0) results from translation termination at the upstream UAG stop codon, while the longer isoform (L-MPZ) results from UAG stop codon readthrough to the downstream UGA termination codon. This RefSeq represents the shorter isoform (MPZ). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC078859.1, K03242.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689600, SAMEA2689602 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTCCTG GGGCTCCCTC ATCCAGCCCC AGCCCTATCC TGGCTGCCCT GCTCTTCTCT 
TCTTTGGTGC TGTCCCCAAC CCTGGCCATT GTGGTTTACA CGGACAGGGA AGTCTATGGT 
GCTGTGGGCT CCCAGGTGAC CCTGCACTGC TCCTTCTGGT CCAGTGAATG GGTCTCAGAT 
GACATCTCTT TTACCTGGCG CTACCAGCCT GAAGGAGGCC GAGATGCCAT TTCAATCTTC 
CACTATGCCA AGGGTCAACC TTACATCGAT GAGGTGGGGA CCTTCAAGGA GCGCATCCAG 
TGGGTAGGGG ACCCTAGCTG GAAGGATGGC TCCATTGTCA TACACAACCT AGACTACAGT 
GACAACGGCA CTTTCACATG TGATGTCAAA AACCCACCGG ACATAGTGGG CAAGACGTCT 
CAGGTCACGC TCTATGTCTT TGAAAAAGTG CCCACTAGGT ATGGGGTGGT GTTGGGAGCC 
GTGATCGGTG GCATCCTCGG GGTGGTGCTG TTGCTGCTGT TGCTCTTCTA CCTGATCCGG 
TACTGCTGGC TGCGCAGGCA GGCTGCCCTG CAGAGGAGGC TCAGTGCCAT GGAGAAGGGG 
AAATTTCACA AGTCTTCTAA GGACTCCTCG AAGCGCGGGC GGCAGACGCC AGTGCTGTAT 
GCCATGCTGG ACCACAGCCG AAGCACCAAA GCTGCCAGTG AGAAGAAATC TAAAGGGCTG 
GGGGAGTCTC GCAAGGATAA GAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058723.2
CDS	171..917
Translation	MAPGAPSSSPSPILAALLFSSLVLSPTLAIVVYTDREVYGAVGSQVTLHCSFWSSEWVSDDISFTWRYQPEGGRDAISIFHYAKGQPYIDEVGTFKERIQWVGDPSWKDGSIVIHNLDYSDNGTFTCDVKNPPDIVGKTSQVTLYVFEKVPTRYGVVLGAVIGGILGVVLLLLLLFYLIRYCWLRRQAALQRRLSAMEKGKFHKSSKDSSKRGRQTPVLYAMLDHSRSTKAASEKKSKGLGESRKDKK

Target ORF information:

RefSeq Version	NM_017027.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus myelin protein zero (Mpz), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017027.2

ORF Insert Sequence:

ATGGCTCCTG GGGCTCCCTC ATCCAGCCCC AGCCCTATCC TGGCTGCCCT GCTCTTCTCT 
TCTTTGGTGC TGTCCCCAAC CCTGGCCATT GTGGTTTACA CGGACAGGGA AGTCTATGGT 
GCTGTGGGCT CCCAGGTGAC CCTGCACTGC TCCTTCTGGT CCAGTGAATG GGTCTCAGAT 
GACATCTCTT TTACCTGGCG CTACCAGCCT GAAGGAGGCC GAGATGCCAT TTCAATCTTC 
CACTATGCCA AGGGTCAACC TTACATCGAT GAGGTGGGGA CCTTCAAGGA GCGCATCCAG 
TGGGTAGGGG ACCCTAGCTG GAAGGATGGC TCCATTGTCA TACACAACCT AGACTACAGT 
GACAACGGCA CTTTCACATG TGATGTCAAA AACCCACCGG ACATAGTGGG CAAGACGTCT 
CAGGTCACGC TCTATGTCTT TGAAAAAGTG CCCACTAGGT ATGGGGTGGT GTTGGGAGCC 
GTGATCGGTG GCATCCTCGG GGTGGTGCTG TTGCTGCTGT TGCTCTTCTA CCTGATCCGG 
TACTGCTGGC TGCGCAGGCA GGCTGCCCTG CAGAGGAGGC TCAGTGCCAT GGAGAAGGGG 
AAATTTCACA AGTCTTCTAA GGACTCCTCG AAGCGCGGGC GGCAGACGCC AGTGCTGTAT 
GCCATGCTGG ACCACAGCCG AAGCACCAAA GCTGCCAGTG AGAAGAAATC TAAAGGGCTG 
GGGGAGTCTC GCAAGGATAA GAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phylogenetically Conserved Sequences Around Myelin P0 Stop Codon are Essential for Translational Readthrough to Produce L-MPZ.
Neurochemical research43(1)227-237(2018 Jan)
Yamaguchi Y,Baba H

Electrical stimulation enhances peripheral nerve regeneration after crush injury in rats.
Molecular medicine reports7(5)1523-7(2013 May)
Zhang X,Xin N,Tong L,Tong XJ

L-MPZ, a novel isoform of myelin P0, is produced by stop codon readthrough.
The Journal of biological chemistry287(21)17765-76(2012 May)
Yamaguchi Y,Hayashi A,Campagnoni CW,Kimura A,Inuzuka T,Baba H

Misreading of termination codons in eukaryotes by natural nonsense suppressor tRNAs.
Nucleic acids research29(23)4767-82(2001 Dec)
Beier H,Grimm M

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Mpz cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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