Ldhb cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ldhb
Entrez Gene ID	16832
Full Name	lactate dehydrogenase B
Synonyms	AI790582,H-Ldh,LDH-B,LDH-H,Ldh-2,Ldh2
General protein information	Preferred Names lactate dehydrogenase B Names L-lactate dehydrogenase B chain LDH heart subunit lactate dehydrogenase 2, B chain
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 6 Map Location: 6 G2\|6 74.17 cM
Summary	This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have also been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes 1 and 19. [provided by RefSeq, Feb 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001302765.1	NP_001289694.1	L-lactate dehydrogenase B chain isoform 2
NM_001316322.1	NP_001303251.1	L-lactate dehydrogenase B chain isoform Ldhbx
NM_008492.3	NP_032518.1	L-lactate dehydrogenase B chain isoform Ldhb

Pathways from BioSystems

Homologies

Homo sapiens (human)	LDHB	NP_002291.1
Bos taurus (cattle)	LDHB	NP_776525.1
Danio rerio (zebrafish)	ldhbb	NP_001002474.1
Danio rerio (zebrafish)	ldhba	NP_571322.1
Xenopus tropicalis (tropical clawed frog)	ldhb	NP_988882.1
Pan troglodytes (chimpanzee)	LDHB	NP_001092031.1
	LDHB	XP_001094453.2
Canis lupus familiaris (dog)	LDHB	NP_001239093.1
Rattus norvegicus (Norway rat)	Ldhb	NP_036727.1
Gallus gallus (chicken)	LDHB	NP_989508.1
Mus musculus (house mouse)	Ldhb	NP_032518.1

Gene Ontology
Bibliography

Related articles in PubMed

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Bulusu V, Prior N, Snaebjornsson MT, Kuehne A, Sonnen KF, Kress J, Stein F, Schultz C, Sauer U, Aulehla A
Developmental cell40(4)331-341.e4(2017 02)

Exercise Inducible Lactate Dehydrogenase B Regulates Mitochondrial Function in Skeletal Muscle.
Liang X, Liu L, Fu T, Zhou Q, Zhou D, Xiao L, Liu J, Kong Y, Xie H, Yi F, Lai L, Vega RB, Kelly DP, Smith SR, Gan Z
The Journal of biological chemistry291(49)25306-25318(2016 Dec)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Ribosomal readthrough at a short UGA stop codon context triggers dual localization of metabolic enzymes in Fungi and animals.
Stiebler AC, Freitag J, Schink KO, Stehlik T, Tillmann BA, Ast J, B?lker M
PLoS genetics10(10)e1004685(2014 Oct)

Peroxisomal lactate dehydrogenase is generated by translational readthrough in mammals.
Schueren F, Lingner T, George R, Hofhuis J, Dickel C, G?rtner J, Thoms S
eLife3e03640(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these results identified a previously unrecognized Ldhb-driven alteration in muscle mitochondrial function and suggested a mechanism for the adaptive metabolic response induced by exercise training.
Title: Exercise Inducible Lactate Dehydrogenase B Regulates Mitochondrial Function in Skeletal Muscle.

drs regulates glucose metabolism via LDH-B. Downregulating drs may contribute to the Warburg effect, which is closely associated with malignant progression of cancer cells.
Title: The drs tumor suppressor regulates glucose metabolism via lactate dehydrogenase-B.

Findings suggest that RANKL protein-induced lactate dehydrogenase (LDH) activation stimulates glycolytic and mitochondrial respiratory metabolism via transcription factor NFATc1 signaling.
Title: Accelerated Lactate Dehydrogenase Activity Potentiates Osteoclastogenesis via NFATc1 Signaling.

Exercise-induced changes in tumour LDH-B and MCT1 expression are modulated by oestrogen-related receptor alpha in breast cancer-bearing BALB/c mice
Title: Exercise-induced changes in tumour LDH-B and MCT1 expression are modulated by oestrogen-related receptor alpha in breast cancer-bearing BALB/c mice.

Data show that PI3K/Akt signalling pathway and lactate dehydrogenase (LDH) play a crucial role in increase of cardiac ATP-binding cassette transporter sub-family C member 9 (SUR2A) induced by in vivo exposure to 18% oxygen.
Title: Mild hypoxia in vivo regulates cardioprotective SUR2A: A role for Akt and LDH.

The following Ldhb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ldhb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu02053	NM_008492.3 Latest version!	Mus musculus lactate dehydrogenase B (Ldhb), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu68126	NM_001302765.1 Latest version!	Mus musculus lactate dehydrogenase B (Ldhb), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu83351	NM_001316322.1 Latest version!	Mus musculus lactate dehydrogenase B (Ldhb), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ldhb ( NM_008492.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032518.1 Mus musculus lactate dehydrogenase B (Ldhb), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu02053
Clone ID Related Accession (Same CDS sequence)	NM_008492.3
Accession Version	NM_008492.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	L-lactate dehydrogenase B chain isoform Ldhb
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK019391.1 and BC046755.1. On Nov 13, 2014 this sequence version replaced NM_008492.2. Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (Ldhb) results from translation termination at the upstream UGA stop codon, while the longer isoform (Ldhbx) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the shorter isoform (Ldhb), which is localized in the cytosol. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAACCC TTAAGGAGAA GCTCATTGCG TCCGTTGCAG ATGATGAGGC TGCCGTCCCG 
AACAACAAGA TCACTGTAGT GGGCGTTGGA CAAGTGGGTA TGGCATGTGC CATCAGCATT 
CTGGGAAAGT CTCTGGCTGA TGAACTTGCC CTGGTGGATG TGTTGGAAGA CAAGCTCAAA 
GGAGAGATGA TGGACCTGCA GCACGGGAGC TTGTTCCTCC AGACTCCGAA AATTGTGGCC 
GATAAAGATT ACTCTGTGAC AGCCAACTCT AAGATTGTGG TGGTGACGGC AGGAGTCCGC 
CAGCAGGAGG GGGAGAGTCG GCTCAACCTG GTGCAGAGAA ATGTCAACGT GTTCAAGTTC 
ATCATTCCTC AGATCGTCAA GTACAGCCCT GACTGCACCA TCATCGTGGT TTCCAACCCA 
GTGGATATTC TGACTTACGT CACCTGGAAA CTGAGCGGGC TACCTAAGCA CCGTGTGATT 
GGAAGCGGAT GCAATCTGGA TTCTGCTCGA TTCCGCTACC TCATGGCAGA GAAGCTTGGC 
ATTCATCCCA GCAGCTGCCA CGGATGGATC CTGGGCGAGC ATGGAGACTC CAGTGTGGCT 
GTGTGGAGCG GGGTGAATGT GGCAGGAGTC TCCCTCCAGG AACTGAATCC AGAAATGGGG 
ACAGACAATG ACAGTGAGAA CTGGAAGGAG GTGCATAAGA TGGTGGTGGA CAGTGCCTAT 
GAAGTCATCA AGCTCAAAGG CTACACCAAC TGGGCCATCG GCCTGAGCGT GGCTGACCTC 
ATCGAGTCCA TGCTGAAAAA CCTCTCCCGG ATTCACCCCG TGTCTACCAT GGTGAAGGGA 
ATGTACGGCA TTGAGAATGA AGTCTTCCTC AGTCTCCCGT GCATCCTCAA TGCTCGGGGG 
CTGACCAGCG TCATCAATCA GAAGCTGAAG GACGATGAGG TCGCTCAGCT CAGGAAGAGT 
GCGGACACCC TGTGGGACAT CCAGAAAGAC CTCAAAGACC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032518.1
CDS	112..1116
Translation	MATLKEKLIASVADDEAAVPNNKITVVGVGQVGMACAISILGKSLADELALVDVLEDKLKGEMMDLQHGSLFLQTPKIVADKDYSVTANSKIVVVTAGVRQQEGESRLNLVQRNVNVFKFIIPQIVKYSPDCTIIVVSNPVDILTYVTWKLSGLPKHRVIGSGCNLDSARFRYLMAEKLGIHPSSCHGWILGEHGDSSVAVWSGVNVAGVSLQELNPEMGTDNDSENWKEVHKMVVDSAYEVIKLKGYTNWAIGLSVADLIESMLKNLSRIHPVSTMVKGMYGIENEVFLSLPCILNARGLTSVINQKLKDDEVAQLRKSADTLWDIQKDLKDL

Target ORF information:

RefSeq Version	NM_008492.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus lactate dehydrogenase B (Ldhb), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_008492.3

ORF Insert Sequence:

ATGGCAACCC TTAAGGAGAA GCTCATTGCG TCCGTTGCAG ATGATGAGGC TGCCGTCCCG 
AACAACAAGA TCACTGTAGT GGGCGTTGGA CAAGTGGGTA TGGCATGTGC CATCAGCATT 
CTGGGAAAGT CTCTGGCTGA TGAACTTGCC CTGGTGGATG TGTTGGAAGA CAAGCTCAAA 
GGAGAGATGA TGGACCTGCA GCACGGGAGC TTGTTCCTCC AGACTCCGAA AATTGTGGCC 
GATAAAGATT ACTCTGTGAC AGCCAACTCT AAGATTGTGG TGGTGACGGC AGGAGTCCGC 
CAGCAGGAGG GGGAGAGTCG GCTCAACCTG GTGCAGAGAA ATGTCAACGT GTTCAAGTTC 
ATCATTCCTC AGATCGTCAA GTACAGCCCT GACTGCACCA TCATCGTGGT TTCCAACCCA 
GTGGATATTC TGACTTACGT CACCTGGAAA CTGAGCGGGC TACCTAAGCA CCGTGTGATT 
GGAAGCGGAT GCAATCTGGA TTCTGCTCGA TTCCGCTACC TCATGGCAGA GAAGCTTGGC 
ATTCATCCCA GCAGCTGCCA CGGATGGATC CTGGGCGAGC ATGGAGACTC CAGTGTGGCT 
GTGTGGAGCG GGGTGAATGT GGCAGGAGTC TCCCTCCAGG AACTGAATCC AGAAATGGGG 
ACAGACAATG ACAGTGAGAA CTGGAAGGAG GTGCATAAGA TGGTGGTGGA CAGTGCCTAT 
GAAGTCATCA AGCTCAAAGG CTACACCAAC TGGGCCATCG GCCTGAGCGT GGCTGACCTC 
ATCGAGTCCA TGCTGAAAAA CCTCTCCCGG ATTCACCCCG TGTCTACCAT GGTGAAGGGA 
ATGTACGGCA TTGAGAATGA AGTCTTCCTC AGTCTCCCGT GCATCCTCAA TGCTCGGGGG 
CTGACCAGCG TCATCAATCA GAAGCTGAAG GACGATGAGG TCGCTCAGCT CAGGAAGAGT 
GCGGACACCC TGTGGGACAT CCAGAAAGAC CTCAAAGACC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ldhb ( NM_001302765.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001289694.1 Mus musculus lactate dehydrogenase B (Ldhb), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu68126
Clone ID Related Accession (Same CDS sequence)	NM_001302765.1
Accession Version	NM_001302765.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	L-lactate dehydrogenase B chain isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK019391.1, CX208147.1 and BC046755.1. Transcript Variant: This variant (2) lacks an exon in the 3' coding region, which results in a frameshift and a different 3' UTR compared to variant 1. The encoded isoform (2) is shorter with a distinct C-terminus compared to isoform Ldhb. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAACCC TTAAGGAGAA GCTCATTGCG TCCGTTGCAG ATGATGAGGC TGCCGTCCCG 
AACAACAAGA TCACTGTAGT GGGCGTTGGA CAAGTGGGTA TGGCATGTGC CATCAGCATT 
CTGGGAAAGT CTCTGGCTGA TGAACTTGCC CTGGTGGATG TGTTGGAAGA CAAGCTCAAA 
GGAGAGATGA TGGACCTGCA GCACGGGAGC TTGTTCCTCC AGACTCCGAA AATTGTGGCC 
GATAAAGATT ACTCTGTGAC AGCCAACTCT AAGATTGTGG TGGTGACGGC AGGAGTCCGC 
CAGCAGGAGG GGGAGAGTCG GCTCAACCTG GTGCAGAGAA ATGTCAACGT GTTCAAGTTC 
ATCATTCCTC AGATCGTCAA GTACAGCCCT GACTGCACCA TCATCGTGGT TTCCAACCCA 
GTGGATATTC TGACTTACGT CACCTGGAAA CTGAGCGGGC TACCTAAGCA CCGTGTGATT 
GGAAGCGGAT GCAATCTGGA TTCTGCTCGA TTCCGCTACC TCATGGCAGA GAAGCTTGGC 
ATTCATCCCA GCAGCTGCCA CGGATGGATC CTGGGCGAGC ATGGAGACTC CAGTGTGGCT 
GTGTGGAGCG GGGTGAATGT GGCAGGAGTC TCCCTCCAGG AACTGAATCC AGAAATGGGG 
ACAGACAATG ACAGTGAGAA CTGGAAGGAG GTGCATAAGA TGGTGGTGGA CAGGGAATGT 
ACGGCATTGA GAATGAAGTC TTCCTCAGTC TCCCGTGCAT CCTCAATGCT CGGGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289694.1
CDS	112..891
Translation	MATLKEKLIASVADDEAAVPNNKITVVGVGQVGMACAISILGKSLADELALVDVLEDKLKGEMMDLQHGSLFLQTPKIVADKDYSVTANSKIVVVTAGVRQQEGESRLNLVQRNVNVFKFIIPQIVKYSPDCTIIVVSNPVDILTYVTWKLSGLPKHRVIGSGCNLDSARFRYLMAEKLGIHPSSCHGWILGEHGDSSVAVWSGVNVAGVSLQELNPEMGTDNDSENWKEVHKMVVDRECTALRMKSSSVSRASSMLGG

Target ORF information:

RefSeq Version	NM_001302765.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus lactate dehydrogenase B (Ldhb), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302765.1

ORF Insert Sequence:

ATGGCAACCC TTAAGGAGAA GCTCATTGCG TCCGTTGCAG ATGATGAGGC TGCCGTCCCG 
AACAACAAGA TCACTGTAGT GGGCGTTGGA CAAGTGGGTA TGGCATGTGC CATCAGCATT 
CTGGGAAAGT CTCTGGCTGA TGAACTTGCC CTGGTGGATG TGTTGGAAGA CAAGCTCAAA 
GGAGAGATGA TGGACCTGCA GCACGGGAGC TTGTTCCTCC AGACTCCGAA AATTGTGGCC 
GATAAAGATT ACTCTGTGAC AGCCAACTCT AAGATTGTGG TGGTGACGGC AGGAGTCCGC 
CAGCAGGAGG GGGAGAGTCG GCTCAACCTG GTGCAGAGAA ATGTCAACGT GTTCAAGTTC 
ATCATTCCTC AGATCGTCAA GTACAGCCCT GACTGCACCA TCATCGTGGT TTCCAACCCA 
GTGGATATTC TGACTTACGT CACCTGGAAA CTGAGCGGGC TACCTAAGCA CCGTGTGATT 
GGAAGCGGAT GCAATCTGGA TTCTGCTCGA TTCCGCTACC TCATGGCAGA GAAGCTTGGC 
ATTCATCCCA GCAGCTGCCA CGGATGGATC CTGGGCGAGC ATGGAGACTC CAGTGTGGCT 
GTGTGGAGCG GGGTGAATGT GGCAGGAGTC TCCCTCCAGG AACTGAATCC AGAAATGGGG 
ACAGACAATG ACAGTGAGAA CTGGAAGGAG GTGCATAAGA TGGTGGTGGA CAGGGAATGT 
ACGGCATTGA GAATGAAGTC TTCCTCAGTC TCCCGTGCAT CCTCAATGCT CGGGGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ldhb ( NM_001316322.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001303251.1 Mus musculus lactate dehydrogenase B (Ldhb), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu83351
Clone ID Related Accession (Same CDS sequence)	NM_001316322.1
Accession Version	NM_001316322.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Mus musculus(house mouse)
Product	L-lactate dehydrogenase B chain isoform Ldhbx
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK019391.1 and BC046755.1. Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (Ldhb) results from translation termination at the upstream UGA stop codon, while the longer isoform (Ldhbx) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the longer, C-terminally extended isoform (Ldhbx), which is localized in the peroxisomes. As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC046755.1, AK019391.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## stop codon readthrough :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAACCC TTAAGGAGAA GCTCATTGCG TCCGTTGCAG ATGATGAGGC TGCCGTCCCG 
AACAACAAGA TCACTGTAGT GGGCGTTGGA CAAGTGGGTA TGGCATGTGC CATCAGCATT 
CTGGGAAAGT CTCTGGCTGA TGAACTTGCC CTGGTGGATG TGTTGGAAGA CAAGCTCAAA 
GGAGAGATGA TGGACCTGCA GCACGGGAGC TTGTTCCTCC AGACTCCGAA AATTGTGGCC 
GATAAAGATT ACTCTGTGAC AGCCAACTCT AAGATTGTGG TGGTGACGGC AGGAGTCCGC 
CAGCAGGAGG GGGAGAGTCG GCTCAACCTG GTGCAGAGAA ATGTCAACGT GTTCAAGTTC 
ATCATTCCTC AGATCGTCAA GTACAGCCCT GACTGCACCA TCATCGTGGT TTCCAACCCA 
GTGGATATTC TGACTTACGT CACCTGGAAA CTGAGCGGGC TACCTAAGCA CCGTGTGATT 
GGAAGCGGAT GCAATCTGGA TTCTGCTCGA TTCCGCTACC TCATGGCAGA GAAGCTTGGC 
ATTCATCCCA GCAGCTGCCA CGGATGGATC CTGGGCGAGC ATGGAGACTC CAGTGTGGCT 
GTGTGGAGCG GGGTGAATGT GGCAGGAGTC TCCCTCCAGG AACTGAATCC AGAAATGGGG 
ACAGACAATG ACAGTGAGAA CTGGAAGGAG GTGCATAAGA TGGTGGTGGA CAGTGCCTAT 
GAAGTCATCA AGCTCAAAGG CTACACCAAC TGGGCCATCG GCCTGAGCGT GGCTGACCTC 
ATCGAGTCCA TGCTGAAAAA CCTCTCCCGG ATTCACCCCG TGTCTACCAT GGTGAAGGGA 
ATGTACGGCA TTGAGAATGA AGTCTTCCTC AGTCTCCCGT GCATCCTCAA TGCTCGGGGG 
CTGACCAGCG TCATCAATCA GAAGCTGAAG GACGATGAGG TCGCTCAGCT CAGGAAGAGT 
GCGGACACCC TGTGGGACAT CCAGAAAGAC CTCAAAGACC TGTGACTGCC AGTCTCTAGG 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303251.1
CDS	112..1137
Translation	MATLKEKLIASVADDEAAVPNNKITVVGVGQVGMACAISILGKSLADELALVDVLEDKLKGEMMDLQHGSLFLQTPKIVADKDYSVTANSKIVVVTAGVRQQEGESRLNLVQRNVNVFKFIIPQIVKYSPDCTIIVVSNPVDILTYVTWKLSGLPKHRVIGSGCNLDSARFRYLMAEKLGIHPSSCHGWILGEHGDSSVAVWSGVNVAGVSLQELNPEMGTDNDSENWKEVHKMVVDSAYEVIKLKGYTNWAIGLSVADLIESMLKNLSRIHPVSTMVKGMYGIENEVFLSLPCILNARGLTSVINQKLKDDEVAQLRKSADTLWDIQKDLKDL*LPVSRL

Target ORF information:

RefSeq Version	NM_001316322.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus lactate dehydrogenase B (Ldhb), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316322.1

ORF Insert Sequence:

ATGGCAACCC TTAAGGAGAA GCTCATTGCG TCCGTTGCAG ATGATGAGGC TGCCGTCCCG 
AACAACAAGA TCACTGTAGT GGGCGTTGGA CAAGTGGGTA TGGCATGTGC CATCAGCATT 
CTGGGAAAGT CTCTGGCTGA TGAACTTGCC CTGGTGGATG TGTTGGAAGA CAAGCTCAAA 
GGAGAGATGA TGGACCTGCA GCACGGGAGC TTGTTCCTCC AGACTCCGAA AATTGTGGCC 
GATAAAGATT ACTCTGTGAC AGCCAACTCT AAGATTGTGG TGGTGACGGC AGGAGTCCGC 
CAGCAGGAGG GGGAGAGTCG GCTCAACCTG GTGCAGAGAA ATGTCAACGT GTTCAAGTTC 
ATCATTCCTC AGATCGTCAA GTACAGCCCT GACTGCACCA TCATCGTGGT TTCCAACCCA 
GTGGATATTC TGACTTACGT CACCTGGAAA CTGAGCGGGC TACCTAAGCA CCGTGTGATT 
GGAAGCGGAT GCAATCTGGA TTCTGCTCGA TTCCGCTACC TCATGGCAGA GAAGCTTGGC 
ATTCATCCCA GCAGCTGCCA CGGATGGATC CTGGGCGAGC ATGGAGACTC CAGTGTGGCT 
GTGTGGAGCG GGGTGAATGT GGCAGGAGTC TCCCTCCAGG AACTGAATCC AGAAATGGGG 
ACAGACAATG ACAGTGAGAA CTGGAAGGAG GTGCATAAGA TGGTGGTGGA CAGTGCCTAT 
GAAGTCATCA AGCTCAAAGG CTACACCAAC TGGGCCATCG GCCTGAGCGT GGCTGACCTC 
ATCGAGTCCA TGCTGAAAAA CCTCTCCCGG ATTCACCCCG TGTCTACCAT GGTGAAGGGA 
ATGTACGGCA TTGAGAATGA AGTCTTCCTC AGTCTCCCGT GCATCCTCAA TGCTCGGGGG 
CTGACCAGCG TCATCAATCA GAAGCTGAAG GACGATGAGG TCGCTCAGCT CAGGAAGAGT 
GCGGACACCC TGTGGGACAT CCAGAAAGAC CTCAAAGACC TGTGACTGCC AGTCTCTAGG 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Spatiotemporal Analysis of a Glycolytic Activity Gradient Linked to Mouse Embryo Mesoderm Development.
Developmental cell40(4)331-341.e4(2017 02)
Bulusu V,Prior N,Snaebjornsson MT,Kuehne A,Sonnen KF,Kress J,Stein F,Schultz C,Sauer U,Aulehla A

Exercise Inducible Lactate Dehydrogenase B Regulates Mitochondrial Function in Skeletal Muscle.
The Journal of biological chemistry291(49)25306-25318(2016 Dec)
Liang X,Liu L,Fu T,Zhou Q,Zhou D,Xiao L,Liu J,Kong Y,Xie H,Yi F,Lai L,Vega RB,Kelly DP,Smith SR,Gan Z

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
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Ldhb cDNA ORF clone, Mus musculus(house mouse)

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