LDHB cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	LDHB
Entrez Gene ID	3945
Full Name	lactate dehydrogenase B
Synonyms	HEL-S-281,LDH-B,LDH-H,LDHBD,TRG-5
General protein information	Preferred Names lactate dehydrogenase B Names L-lactate dehydrogenase B chain LDH heart subunit epididymis secretory protein Li 281 lactate dehydrogenase H chain renal carcinoma antigen NY-REN-46 testicular secretory protein Li 25
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12p12.1
Summary	This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13. [provided by RefSeq, Feb 2016].
Disorder MIM:	150100
Disorder Html:	[Lactate dehydrogenase-B deficiency], 614128 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006719074.2	XP_006719137.1	L-lactate dehydrogenase B chain isoform X1
NM_001174097.1	NP_001167568.1	L-lactate dehydrogenase B chain
NM_001315537.1	NP_001302466.1	L-lactate dehydrogenase B chain isoform LDHBx
NM_002300.7	NP_002291.1	L-lactate dehydrogenase B chain isoform LDHB
NM_002300.6	NP_002291.1	L-lactate dehydrogenase B chain
NM_001174097.2	NP_001167568.1	L-lactate dehydrogenase B chain isoform LDHB

Pathways from BioSystems

Homologies

Homo sapiens (human)	LDHB	NP_002291.1
Bos taurus (cattle)	LDHB	NP_776525.1
Danio rerio (zebrafish)	ldhbb	NP_001002474.1
Danio rerio (zebrafish)	ldhba	NP_571322.1
Xenopus tropicalis (tropical clawed frog)	ldhb	NP_988882.1
Pan troglodytes (chimpanzee)	LDHB	NP_001092031.1
	LDHB	XP_001094453.2
Canis lupus familiaris (dog)	LDHB	NP_001239093.1
Rattus norvegicus (Norway rat)	Ldhb	NP_036727.1
Gallus gallus (chicken)	LDHB	NP_989508.1
Mus musculus (house mouse)	Ldhb	NP_032518.1

Gene Ontology
Bibliography

Related articles in PubMed

Ribosomal readthrough at a short UGA stop codon context triggers dual localization of metabolic enzymes in Fungi and animals.
Stiebler AC, Freitag J, Schink KO, Stehlik T, Tillmann BA, Ast J, B?lker M
PLoS genetics10(10)e1004685(2014 Oct)

Peroxisomal lactate dehydrogenase is generated by translational readthrough in mammals.
Schueren F, Lingner T, George R, Hofhuis J, Dickel C, G?rtner J, Thoms S
eLife3e03640(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this study shows that low base line lactate dehydrogenase is linked with a better tumor response, and that prolonged overall survival and progression-free survival are observed for patients under ipilimumab treatment with no increase in lactate dehydrogenase
Title: Use of LDH and autoimmune side effects to predict response to ipilimumab treatment.

our study examines the inclusion of salivary LDH as potential diagnostic parameter and therapeutic index in Oral squamous cell carcinoma
Title: Equating salivary lactate dehydrogenase (LDH) with LDH-5 expression in patients with oral squamous cell carcinoma: An insight into metabolic reprogramming of cancer cell as a predictor of aggressive phenotype.

addition of exogenous lactic acid to growth media was sufficient to induce cell death that could be inhibited by the expression of LDHB. the results suggest that lactate dehydrogenase is a general suppressor of programmed cell death in yeast.
Title: Inhibition of stress mediated cell death by human lactate dehydrogenase B in yeast.

The aim of this study was to demonstrate the effects of 6-week low-intensity training on changes in indicators of aerobic capacity and on HSPA1A, HSPB1, and LDHb expression in white blood cells in high level rowers.
Title: Effects of 6-week specific low-intensity training on selected aerobic capacity parameters and HSPA1A, HSPB1, and LDHb gene expression in high-level rowers.

Lactate dehydrogenase B is associated with the response to neoadjuvant chemotherapy in oral squamous cell carcinoma.
Title: Lactate dehydrogenase B is associated with the response to neoadjuvant chemotherapy in oral squamous cell carcinoma.

The following LDHB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LDHB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu08149	NM_002300.7 Latest version!	Homo sapiens lactate dehydrogenase B (LDHB), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$139.50-$195.30 $279.00
OHu08149	NM_001174097.2 Latest version!	Homo sapiens lactate dehydrogenase B (LDHB), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$139.50-$195.30 $279.00
OHu100963	NM_001315537.1 Latest version!	Homo sapiens lactate dehydrogenase B (LDHB), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu46104	XM_006719074.2 Latest version!	Homo sapiens lactate dehydrogenase B (LDHB), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu08149	NM_002300.6	Homo sapiens lactate dehydrogenase B (LDHB), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$139.50-$195.30 $279.00
OHu08149	NM_001174097.1	Homo sapiens lactate dehydrogenase B (LDHB), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$139.50-$195.30 $279.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LDHB ( NM_001174097.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001167568.1 Homo sapiens lactate dehydrogenase B (LDHB), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu08149
Clone ID Related Accession (Same CDS sequence)	NM_001174097.1 , NM_002300.7 , NM_002300.6 , NM_001174097.2
Accession Version	NM_001174097.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	L-lactate dehydrogenase B chain
Comment

ATGGCAACTC TTAAGGAAAA ACTCATTGCA CCAGTTGCGG AAGAAGAGGC AACAGTTCCA 
AACAATAAGA TCACTGTAGT GGGTGTTGGA CAAGTTGGTA TGGCGTGTGC TATCAGCATT 
CTGGGAAAGT CTCTGGCTGA TGAACTTGCT CTTGTGGATG TTTTGGAAGA TAAGCTTAAA 
GGAGAAATGA TGGATCTGCA GCATGGGAGC TTATTTCTTC AGACACCTAA AATTGTGGCA 
GATAAAGATT ATTCTGTGAC CGCCAATTCT AAGATTGTAG TGGTAACTGC AGGAGTCCGT 
CAGCAAGAAG GGGAGAGTCG GCTCAATCTG GTGCAGAGAA ATGTTAATGT CTTCAAATTC 
ATTATTCCTC AGATCGTCAA GTACAGTCCT GATTGCATCA TAATTGTGGT TTCCAACCCA 
GTGGACATTC TTACGTATGT TACCTGGAAA CTAAGTGGAT TACCCAAACA CCGCGTGATT 
GGAAGTGGAT GTAATCTGGA TTCTGCTAGA TTTCGCTACC TTATGGCTGA AAAACTTGGC 
ATTCATCCCA GCAGCTGCCA TGGATGGATT TTGGGGGAAC ATGGCGACTC AAGTGTGGCT 
GTGTGGAGTG GTGTGAATGT GGCAGGTGTT TCTCTCCAGG AATTGAATCC AGAAATGGGA 
ACTGACAATG ATAGTGAAAA TTGGAAGGAA GTGCATAAGA TGGTGGTTGA AAGTGCCTAT 
GAAGTCATCA AGCTAAAAGG ATATACCAAC TGGGCTATTG GATTAAGTGT GGCTGATCTT 
ATTGAATCCA TGTTGAAAAA TCTATCCAGG ATTCATCCCG TGTCAACAAT GGTAAAGGGG 
ATGTATGGCA TTGAGAATGA AGTCTTCCTG AGCCTTCCAT GTATCCTCAA TGCCCGGGGA 
TTAACCAGCG TTATCAACCA GAAGCTAAAG GATGATGAGG TTGCTCAGCT CAAGAAAAGT 
GCAGATACCC TGTGGGACAT CCAGAAGGAC CTAAAAGACC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001167568.1
Translation	MATLKEKLIAPVAEEEATVPNNKITVVGVGQVGMACAISILGKSLADELALVDVLEDKLKGEMMDLQHGSLFLQTPKIVADKDYSVTANSKIVVVTAGVRQQEGESRLNLVQRNVNVFKFIIPQIVKYSPDCIIIVVSNPVDILTYVTWKLSGLPKHRVIGSGCNLDSARFRYLMAEKLGIHPSSCHGWILGEHGDSSVAVWSGVNVAGVSLQELNPEMGTDNDSENWKEVHKMVVESAYEVIKLKGYTNWAIGLSVADLIESMLKNLSRIHPVSTMVKGMYGIENEVFLSLPCILNARGLTSVINQKLKDDEVAQLKKSADTLWDIQKDLKDL

Target ORF information:

RefSeq Version	NM_001174097.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens lactate dehydrogenase B (LDHB), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001174097.1

ORF Insert Sequence:

ATGGCAACTC TTAAGGAAAA ACTCATTGCA CCAGTTGCGG AAGAAGAGGC AACAGTTCCA 
AACAATAAGA TCACTGTAGT GGGTGTTGGA CAAGTTGGTA TGGCGTGTGC TATCAGCATT 
CTGGGAAAGT CTCTGGCTGA TGAACTTGCT CTTGTGGATG TTTTGGAAGA TAAGCTTAAA 
GGAGAAATGA TGGATCTGCA GCATGGGAGC TTATTTCTTC AGACACCTAA AATTGTGGCA 
GATAAAGATT ATTCTGTGAC CGCCAATTCT AAGATTGTAG TGGTAACTGC AGGAGTCCGT 
CAGCAAGAAG GGGAGAGTCG GCTCAATCTG GTGCAGAGAA ATGTTAATGT CTTCAAATTC 
ATTATTCCTC AGATCGTCAA GTACAGTCCT GATTGCATCA TAATTGTGGT TTCCAACCCA 
GTGGACATTC TTACGTATGT TACCTGGAAA CTAAGTGGAT TACCCAAACA CCGCGTGATT 
GGAAGTGGAT GTAATCTGGA TTCTGCTAGA TTTCGCTACC TTATGGCTGA AAAACTTGGC 
ATTCATCCCA GCAGCTGCCA TGGATGGATT TTGGGGGAAC ATGGCGACTC AAGTGTGGCT 
GTGTGGAGTG GTGTGAATGT GGCAGGTGTT TCTCTCCAGG AATTGAATCC AGAAATGGGA 
ACTGACAATG ATAGTGAAAA TTGGAAGGAA GTGCATAAGA TGGTGGTTGA AAGTGCCTAT 
GAAGTCATCA AGCTAAAAGG ATATACCAAC TGGGCTATTG GATTAAGTGT GGCTGATCTT 
ATTGAATCCA TGTTGAAAAA TCTATCCAGG ATTCATCCCG TGTCAACAAT GGTAAAGGGG 
ATGTATGGCA TTGAGAATGA AGTCTTCCTG AGCCTTCCAT GTATCCTCAA TGCCCGGGGA 
TTAACCAGCG TTATCAACCA GAAGCTAAAG GATGATGAGG TTGCTCAGCT CAAGAAAAGT 
GCAGATACCC TGTGGGACAT CCAGAAGGAC CTAAAAGACC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LDHB ( NM_002300.7 ) cDNA ORF clone, Homo sapiens(human) -> NP_002291.1 Homo sapiens lactate dehydrogenase B (LDHB), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu08149
Clone ID Related Accession (Same CDS sequence)	NM_001174097.1 , NM_002300.7 , NM_002300.6 , NM_001174097.2
Accession Version	NM_002300.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Homo sapiens(human)
Product	L-lactate dehydrogenase B chain isoform LDHB
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU540074.1 and BC002362.1. This sequence is a reference standard in the RefSeqGene project. On Oct 9, 2015 this sequence version replaced NM_002300.6. Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (LDHB) results from translation termination at the upstream UGA stop codon, while the longer isoform (LDHBx) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the shorter isoform (LDHB), which is localized in the cytosol. Variants 1 and 2 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM005565.1, SRR1163658.350139.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267764 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAACTC TTAAGGAAAA ACTCATTGCA CCAGTTGCGG AAGAAGAGGC AACAGTTCCA 
AACAATAAGA TCACTGTAGT GGGTGTTGGA CAAGTTGGTA TGGCGTGTGC TATCAGCATT 
CTGGGAAAGT CTCTGGCTGA TGAACTTGCT CTTGTGGATG TTTTGGAAGA TAAGCTTAAA 
GGAGAAATGA TGGATCTGCA GCATGGGAGC TTATTTCTTC AGACACCTAA AATTGTGGCA 
GATAAAGATT ATTCTGTGAC CGCCAATTCT AAGATTGTAG TGGTAACTGC AGGAGTCCGT 
CAGCAAGAAG GGGAGAGTCG GCTCAATCTG GTGCAGAGAA ATGTTAATGT CTTCAAATTC 
ATTATTCCTC AGATCGTCAA GTACAGTCCT GATTGCATCA TAATTGTGGT TTCCAACCCA 
GTGGACATTC TTACGTATGT TACCTGGAAA CTAAGTGGAT TACCCAAACA CCGCGTGATT 
GGAAGTGGAT GTAATCTGGA TTCTGCTAGA TTTCGCTACC TTATGGCTGA AAAACTTGGC 
ATTCATCCCA GCAGCTGCCA TGGATGGATT TTGGGGGAAC ATGGCGACTC AAGTGTGGCT 
GTGTGGAGTG GTGTGAATGT GGCAGGTGTT TCTCTCCAGG AATTGAATCC AGAAATGGGA 
ACTGACAATG ATAGTGAAAA TTGGAAGGAA GTGCATAAGA TGGTGGTTGA AAGTGCCTAT 
GAAGTCATCA AGCTAAAAGG ATATACCAAC TGGGCTATTG GATTAAGTGT GGCTGATCTT 
ATTGAATCCA TGTTGAAAAA TCTATCCAGG ATTCATCCCG TGTCAACAAT GGTAAAGGGG 
ATGTATGGCA TTGAGAATGA AGTCTTCCTG AGCCTTCCAT GTATCCTCAA TGCCCGGGGA 
TTAACCAGCG TTATCAACCA GAAGCTAAAG GATGATGAGG TTGCTCAGCT CAAGAAAAGT 
GCAGATACCC TGTGGGACAT CCAGAAGGAC CTAAAAGACC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002291.1
CDS	112..1116
Translation	MATLKEKLIAPVAEEEATVPNNKITVVGVGQVGMACAISILGKSLADELALVDVLEDKLKGEMMDLQHGSLFLQTPKIVADKDYSVTANSKIVVVTAGVRQQEGESRLNLVQRNVNVFKFIIPQIVKYSPDCIIIVVSNPVDILTYVTWKLSGLPKHRVIGSGCNLDSARFRYLMAEKLGIHPSSCHGWILGEHGDSSVAVWSGVNVAGVSLQELNPEMGTDNDSENWKEVHKMVVESAYEVIKLKGYTNWAIGLSVADLIESMLKNLSRIHPVSTMVKGMYGIENEVFLSLPCILNARGLTSVINQKLKDDEVAQLKKSADTLWDIQKDLKDL

Target ORF information:

RefSeq Version	NM_002300.7
Organism	Homo sapiens(human)
Definition	Homo sapiens lactate dehydrogenase B (LDHB), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002300.7

ORF Insert Sequence:

ATGGCAACTC TTAAGGAAAA ACTCATTGCA CCAGTTGCGG AAGAAGAGGC AACAGTTCCA 
AACAATAAGA TCACTGTAGT GGGTGTTGGA CAAGTTGGTA TGGCGTGTGC TATCAGCATT 
CTGGGAAAGT CTCTGGCTGA TGAACTTGCT CTTGTGGATG TTTTGGAAGA TAAGCTTAAA 
GGAGAAATGA TGGATCTGCA GCATGGGAGC TTATTTCTTC AGACACCTAA AATTGTGGCA 
GATAAAGATT ATTCTGTGAC CGCCAATTCT AAGATTGTAG TGGTAACTGC AGGAGTCCGT 
CAGCAAGAAG GGGAGAGTCG GCTCAATCTG GTGCAGAGAA ATGTTAATGT CTTCAAATTC 
ATTATTCCTC AGATCGTCAA GTACAGTCCT GATTGCATCA TAATTGTGGT TTCCAACCCA 
GTGGACATTC TTACGTATGT TACCTGGAAA CTAAGTGGAT TACCCAAACA CCGCGTGATT 
GGAAGTGGAT GTAATCTGGA TTCTGCTAGA TTTCGCTACC TTATGGCTGA AAAACTTGGC 
ATTCATCCCA GCAGCTGCCA TGGATGGATT TTGGGGGAAC ATGGCGACTC AAGTGTGGCT 
GTGTGGAGTG GTGTGAATGT GGCAGGTGTT TCTCTCCAGG AATTGAATCC AGAAATGGGA 
ACTGACAATG ATAGTGAAAA TTGGAAGGAA GTGCATAAGA TGGTGGTTGA AAGTGCCTAT 
GAAGTCATCA AGCTAAAAGG ATATACCAAC TGGGCTATTG GATTAAGTGT GGCTGATCTT 
ATTGAATCCA TGTTGAAAAA TCTATCCAGG ATTCATCCCG TGTCAACAAT GGTAAAGGGG 
ATGTATGGCA TTGAGAATGA AGTCTTCCTG AGCCTTCCAT GTATCCTCAA TGCCCGGGGA 
TTAACCAGCG TTATCAACCA GAAGCTAAAG GATGATGAGG TTGCTCAGCT CAAGAAAAGT 
GCAGATACCC TGTGGGACAT CCAGAAGGAC CTAAAAGACC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LDHB ( NM_002300.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_002291.1 Homo sapiens lactate dehydrogenase B (LDHB), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu08149
Clone ID Related Accession (Same CDS sequence)	NM_001174097.1 , NM_002300.7 , NM_002300.6 , NM_001174097.2
Accession Version	NM_002300.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	L-lactate dehydrogenase B chain
Comment

ATGGCAACTC TTAAGGAAAA ACTCATTGCA CCAGTTGCGG AAGAAGAGGC AACAGTTCCA 
AACAATAAGA TCACTGTAGT GGGTGTTGGA CAAGTTGGTA TGGCGTGTGC TATCAGCATT 
CTGGGAAAGT CTCTGGCTGA TGAACTTGCT CTTGTGGATG TTTTGGAAGA TAAGCTTAAA 
GGAGAAATGA TGGATCTGCA GCATGGGAGC TTATTTCTTC AGACACCTAA AATTGTGGCA 
GATAAAGATT ATTCTGTGAC CGCCAATTCT AAGATTGTAG TGGTAACTGC AGGAGTCCGT 
CAGCAAGAAG GGGAGAGTCG GCTCAATCTG GTGCAGAGAA ATGTTAATGT CTTCAAATTC 
ATTATTCCTC AGATCGTCAA GTACAGTCCT GATTGCATCA TAATTGTGGT TTCCAACCCA 
GTGGACATTC TTACGTATGT TACCTGGAAA CTAAGTGGAT TACCCAAACA CCGCGTGATT 
GGAAGTGGAT GTAATCTGGA TTCTGCTAGA TTTCGCTACC TTATGGCTGA AAAACTTGGC 
ATTCATCCCA GCAGCTGCCA TGGATGGATT TTGGGGGAAC ATGGCGACTC AAGTGTGGCT 
GTGTGGAGTG GTGTGAATGT GGCAGGTGTT TCTCTCCAGG AATTGAATCC AGAAATGGGA 
ACTGACAATG ATAGTGAAAA TTGGAAGGAA GTGCATAAGA TGGTGGTTGA AAGTGCCTAT 
GAAGTCATCA AGCTAAAAGG ATATACCAAC TGGGCTATTG GATTAAGTGT GGCTGATCTT 
ATTGAATCCA TGTTGAAAAA TCTATCCAGG ATTCATCCCG TGTCAACAAT GGTAAAGGGG 
ATGTATGGCA TTGAGAATGA AGTCTTCCTG AGCCTTCCAT GTATCCTCAA TGCCCGGGGA 
TTAACCAGCG TTATCAACCA GAAGCTAAAG GATGATGAGG TTGCTCAGCT CAAGAAAAGT 
GCAGATACCC TGTGGGACAT CCAGAAGGAC CTAAAAGACC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002291.1
Translation	MATLKEKLIAPVAEEEATVPNNKITVVGVGQVGMACAISILGKSLADELALVDVLEDKLKGEMMDLQHGSLFLQTPKIVADKDYSVTANSKIVVVTAGVRQQEGESRLNLVQRNVNVFKFIIPQIVKYSPDCIIIVVSNPVDILTYVTWKLSGLPKHRVIGSGCNLDSARFRYLMAEKLGIHPSSCHGWILGEHGDSSVAVWSGVNVAGVSLQELNPEMGTDNDSENWKEVHKMVVESAYEVIKLKGYTNWAIGLSVADLIESMLKNLSRIHPVSTMVKGMYGIENEVFLSLPCILNARGLTSVINQKLKDDEVAQLKKSADTLWDIQKDLKDL

Target ORF information:

RefSeq Version	NM_002300.6
Organism	Homo sapiens(Human)
Definition	Homo sapiens lactate dehydrogenase B (LDHB), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002300.6

ORF Insert Sequence:

ATGGCAACTC TTAAGGAAAA ACTCATTGCA CCAGTTGCGG AAGAAGAGGC AACAGTTCCA 
AACAATAAGA TCACTGTAGT GGGTGTTGGA CAAGTTGGTA TGGCGTGTGC TATCAGCATT 
CTGGGAAAGT CTCTGGCTGA TGAACTTGCT CTTGTGGATG TTTTGGAAGA TAAGCTTAAA 
GGAGAAATGA TGGATCTGCA GCATGGGAGC TTATTTCTTC AGACACCTAA AATTGTGGCA 
GATAAAGATT ATTCTGTGAC CGCCAATTCT AAGATTGTAG TGGTAACTGC AGGAGTCCGT 
CAGCAAGAAG GGGAGAGTCG GCTCAATCTG GTGCAGAGAA ATGTTAATGT CTTCAAATTC 
ATTATTCCTC AGATCGTCAA GTACAGTCCT GATTGCATCA TAATTGTGGT TTCCAACCCA 
GTGGACATTC TTACGTATGT TACCTGGAAA CTAAGTGGAT TACCCAAACA CCGCGTGATT 
GGAAGTGGAT GTAATCTGGA TTCTGCTAGA TTTCGCTACC TTATGGCTGA AAAACTTGGC 
ATTCATCCCA GCAGCTGCCA TGGATGGATT TTGGGGGAAC ATGGCGACTC AAGTGTGGCT 
GTGTGGAGTG GTGTGAATGT GGCAGGTGTT TCTCTCCAGG AATTGAATCC AGAAATGGGA 
ACTGACAATG ATAGTGAAAA TTGGAAGGAA GTGCATAAGA TGGTGGTTGA AAGTGCCTAT 
GAAGTCATCA AGCTAAAAGG ATATACCAAC TGGGCTATTG GATTAAGTGT GGCTGATCTT 
ATTGAATCCA TGTTGAAAAA TCTATCCAGG ATTCATCCCG TGTCAACAAT GGTAAAGGGG 
ATGTATGGCA TTGAGAATGA AGTCTTCCTG AGCCTTCCAT GTATCCTCAA TGCCCGGGGA 
TTAACCAGCG TTATCAACCA GAAGCTAAAG GATGATGAGG TTGCTCAGCT CAAGAAAAGT 
GCAGATACCC TGTGGGACAT CCAGAAGGAC CTAAAAGACC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LDHB ( NM_001174097.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001167568.1 Homo sapiens lactate dehydrogenase B (LDHB), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu08149
Clone ID Related Accession (Same CDS sequence)	NM_001174097.1 , NM_002300.7 , NM_002300.6 , NM_001174097.2
Accession Version	NM_001174097.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Homo sapiens(human)
Product	L-lactate dehydrogenase B chain isoform LDHB
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL516910.3, CX867875.1 and BC002362.1. On Oct 9, 2015 this sequence version replaced NM_001174097.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (LDHB). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.292875.1, SRR1803615.385955.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267752 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAACTC TTAAGGAAAA ACTCATTGCA CCAGTTGCGG AAGAAGAGGC AACAGTTCCA 
AACAATAAGA TCACTGTAGT GGGTGTTGGA CAAGTTGGTA TGGCGTGTGC TATCAGCATT 
CTGGGAAAGT CTCTGGCTGA TGAACTTGCT CTTGTGGATG TTTTGGAAGA TAAGCTTAAA 
GGAGAAATGA TGGATCTGCA GCATGGGAGC TTATTTCTTC AGACACCTAA AATTGTGGCA 
GATAAAGATT ATTCTGTGAC CGCCAATTCT AAGATTGTAG TGGTAACTGC AGGAGTCCGT 
CAGCAAGAAG GGGAGAGTCG GCTCAATCTG GTGCAGAGAA ATGTTAATGT CTTCAAATTC 
ATTATTCCTC AGATCGTCAA GTACAGTCCT GATTGCATCA TAATTGTGGT TTCCAACCCA 
GTGGACATTC TTACGTATGT TACCTGGAAA CTAAGTGGAT TACCCAAACA CCGCGTGATT 
GGAAGTGGAT GTAATCTGGA TTCTGCTAGA TTTCGCTACC TTATGGCTGA AAAACTTGGC 
ATTCATCCCA GCAGCTGCCA TGGATGGATT TTGGGGGAAC ATGGCGACTC AAGTGTGGCT 
GTGTGGAGTG GTGTGAATGT GGCAGGTGTT TCTCTCCAGG AATTGAATCC AGAAATGGGA 
ACTGACAATG ATAGTGAAAA TTGGAAGGAA GTGCATAAGA TGGTGGTTGA AAGTGCCTAT 
GAAGTCATCA AGCTAAAAGG ATATACCAAC TGGGCTATTG GATTAAGTGT GGCTGATCTT 
ATTGAATCCA TGTTGAAAAA TCTATCCAGG ATTCATCCCG TGTCAACAAT GGTAAAGGGG 
ATGTATGGCA TTGAGAATGA AGTCTTCCTG AGCCTTCCAT GTATCCTCAA TGCCCGGGGA 
TTAACCAGCG TTATCAACCA GAAGCTAAAG GATGATGAGG TTGCTCAGCT CAAGAAAAGT 
GCAGATACCC TGTGGGACAT CCAGAAGGAC CTAAAAGACC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001167568.1
CDS	334..1338
Translation	MATLKEKLIAPVAEEEATVPNNKITVVGVGQVGMACAISILGKSLADELALVDVLEDKLKGEMMDLQHGSLFLQTPKIVADKDYSVTANSKIVVVTAGVRQQEGESRLNLVQRNVNVFKFIIPQIVKYSPDCIIIVVSNPVDILTYVTWKLSGLPKHRVIGSGCNLDSARFRYLMAEKLGIHPSSCHGWILGEHGDSSVAVWSGVNVAGVSLQELNPEMGTDNDSENWKEVHKMVVESAYEVIKLKGYTNWAIGLSVADLIESMLKNLSRIHPVSTMVKGMYGIENEVFLSLPCILNARGLTSVINQKLKDDEVAQLKKSADTLWDIQKDLKDL

Target ORF information:

RefSeq Version	NM_001174097.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lactate dehydrogenase B (LDHB), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001174097.2

ORF Insert Sequence:

ATGGCAACTC TTAAGGAAAA ACTCATTGCA CCAGTTGCGG AAGAAGAGGC AACAGTTCCA 
AACAATAAGA TCACTGTAGT GGGTGTTGGA CAAGTTGGTA TGGCGTGTGC TATCAGCATT 
CTGGGAAAGT CTCTGGCTGA TGAACTTGCT CTTGTGGATG TTTTGGAAGA TAAGCTTAAA 
GGAGAAATGA TGGATCTGCA GCATGGGAGC TTATTTCTTC AGACACCTAA AATTGTGGCA 
GATAAAGATT ATTCTGTGAC CGCCAATTCT AAGATTGTAG TGGTAACTGC AGGAGTCCGT 
CAGCAAGAAG GGGAGAGTCG GCTCAATCTG GTGCAGAGAA ATGTTAATGT CTTCAAATTC 
ATTATTCCTC AGATCGTCAA GTACAGTCCT GATTGCATCA TAATTGTGGT TTCCAACCCA 
GTGGACATTC TTACGTATGT TACCTGGAAA CTAAGTGGAT TACCCAAACA CCGCGTGATT 
GGAAGTGGAT GTAATCTGGA TTCTGCTAGA TTTCGCTACC TTATGGCTGA AAAACTTGGC 
ATTCATCCCA GCAGCTGCCA TGGATGGATT TTGGGGGAAC ATGGCGACTC AAGTGTGGCT 
GTGTGGAGTG GTGTGAATGT GGCAGGTGTT TCTCTCCAGG AATTGAATCC AGAAATGGGA 
ACTGACAATG ATAGTGAAAA TTGGAAGGAA GTGCATAAGA TGGTGGTTGA AAGTGCCTAT 
GAAGTCATCA AGCTAAAAGG ATATACCAAC TGGGCTATTG GATTAAGTGT GGCTGATCTT 
ATTGAATCCA TGTTGAAAAA TCTATCCAGG ATTCATCCCG TGTCAACAAT GGTAAAGGGG 
ATGTATGGCA TTGAGAATGA AGTCTTCCTG AGCCTTCCAT GTATCCTCAA TGCCCGGGGA 
TTAACCAGCG TTATCAACCA GAAGCTAAAG GATGATGAGG TTGCTCAGCT CAAGAAAAGT 
GCAGATACCC TGTGGGACAT CCAGAAGGAC CTAAAAGACC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LDHB ( NM_001315537.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001302466.1 Homo sapiens lactate dehydrogenase B (LDHB), transcript variant 1, mRNA.

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CloneID	OHu100963
Clone ID Related Accession (Same CDS sequence)	NM_001315537.1
Accession Version	NM_001315537.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Homo sapiens(human)
Product	L-lactate dehydrogenase B chain isoform LDHBx
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU540074.1 and BC002362.1. Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (LDHB) results from translation termination at the upstream UGA stop codon, while the longer isoform (LDHBx) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the longer, C-terminally extended isoform (LDHBx), which is localized in the peroxisomes. As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM005565.1, SRR1163658.350139.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267764 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## stop codon readthrough :: PMID: 25247702, 25340584 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAACTC TTAAGGAAAA ACTCATTGCA CCAGTTGCGG AAGAAGAGGC AACAGTTCCA 
AACAATAAGA TCACTGTAGT GGGTGTTGGA CAAGTTGGTA TGGCGTGTGC TATCAGCATT 
CTGGGAAAGT CTCTGGCTGA TGAACTTGCT CTTGTGGATG TTTTGGAAGA TAAGCTTAAA 
GGAGAAATGA TGGATCTGCA GCATGGGAGC TTATTTCTTC AGACACCTAA AATTGTGGCA 
GATAAAGATT ATTCTGTGAC CGCCAATTCT AAGATTGTAG TGGTAACTGC AGGAGTCCGT 
CAGCAAGAAG GGGAGAGTCG GCTCAATCTG GTGCAGAGAA ATGTTAATGT CTTCAAATTC 
ATTATTCCTC AGATCGTCAA GTACAGTCCT GATTGCATCA TAATTGTGGT TTCCAACCCA 
GTGGACATTC TTACGTATGT TACCTGGAAA CTAAGTGGAT TACCCAAACA CCGCGTGATT 
GGAAGTGGAT GTAATCTGGA TTCTGCTAGA TTTCGCTACC TTATGGCTGA AAAACTTGGC 
ATTCATCCCA GCAGCTGCCA TGGATGGATT TTGGGGGAAC ATGGCGACTC AAGTGTGGCT 
GTGTGGAGTG GTGTGAATGT GGCAGGTGTT TCTCTCCAGG AATTGAATCC AGAAATGGGA 
ACTGACAATG ATAGTGAAAA TTGGAAGGAA GTGCATAAGA TGGTGGTTGA AAGTGCCTAT 
GAAGTCATCA AGCTAAAAGG ATATACCAAC TGGGCTATTG GATTAAGTGT GGCTGATCTT 
ATTGAATCCA TGTTGAAAAA TCTATCCAGG ATTCATCCCG TGTCAACAAT GGTAAAGGGG 
ATGTATGGCA TTGAGAATGA AGTCTTCCTG AGCCTTCCAT GTATCCTCAA TGCCCGGGGA 
TTAACCAGCG TTATCAACCA GAAGCTAAAG GATGATGAGG TTGCTCAGCT CAAGAAAAGT 
GCAGATACCC TGTGGGACAT CCAGAAGGAC CTAAAAGACC TGTGACTAGT GAGCTCTAGG 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001302466.1
CDS	112..1137
Translation	MATLKEKLIAPVAEEEATVPNNKITVVGVGQVGMACAISILGKSLADELALVDVLEDKLKGEMMDLQHGSLFLQTPKIVADKDYSVTANSKIVVVTAGVRQQEGESRLNLVQRNVNVFKFIIPQIVKYSPDCIIIVVSNPVDILTYVTWKLSGLPKHRVIGSGCNLDSARFRYLMAEKLGIHPSSCHGWILGEHGDSSVAVWSGVNVAGVSLQELNPEMGTDNDSENWKEVHKMVVESAYEVIKLKGYTNWAIGLSVADLIESMLKNLSRIHPVSTMVKGMYGIENEVFLSLPCILNARGLTSVINQKLKDDEVAQLKKSADTLWDIQKDLKDL*LVSSRL

Target ORF information:

RefSeq Version	NM_001315537.1
Organism	Homo sapiens(human)
Definition	Homo sapiens lactate dehydrogenase B (LDHB), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001315537.1

ORF Insert Sequence:

ATGGCAACTC TTAAGGAAAA ACTCATTGCA CCAGTTGCGG AAGAAGAGGC AACAGTTCCA 
AACAATAAGA TCACTGTAGT GGGTGTTGGA CAAGTTGGTA TGGCGTGTGC TATCAGCATT 
CTGGGAAAGT CTCTGGCTGA TGAACTTGCT CTTGTGGATG TTTTGGAAGA TAAGCTTAAA 
GGAGAAATGA TGGATCTGCA GCATGGGAGC TTATTTCTTC AGACACCTAA AATTGTGGCA 
GATAAAGATT ATTCTGTGAC CGCCAATTCT AAGATTGTAG TGGTAACTGC AGGAGTCCGT 
CAGCAAGAAG GGGAGAGTCG GCTCAATCTG GTGCAGAGAA ATGTTAATGT CTTCAAATTC 
ATTATTCCTC AGATCGTCAA GTACAGTCCT GATTGCATCA TAATTGTGGT TTCCAACCCA 
GTGGACATTC TTACGTATGT TACCTGGAAA CTAAGTGGAT TACCCAAACA CCGCGTGATT 
GGAAGTGGAT GTAATCTGGA TTCTGCTAGA TTTCGCTACC TTATGGCTGA AAAACTTGGC 
ATTCATCCCA GCAGCTGCCA TGGATGGATT TTGGGGGAAC ATGGCGACTC AAGTGTGGCT 
GTGTGGAGTG GTGTGAATGT GGCAGGTGTT TCTCTCCAGG AATTGAATCC AGAAATGGGA 
ACTGACAATG ATAGTGAAAA TTGGAAGGAA GTGCATAAGA TGGTGGTTGA AAGTGCCTAT 
GAAGTCATCA AGCTAAAAGG ATATACCAAC TGGGCTATTG GATTAAGTGT GGCTGATCTT 
ATTGAATCCA TGTTGAAAAA TCTATCCAGG ATTCATCCCG TGTCAACAAT GGTAAAGGGG 
ATGTATGGCA TTGAGAATGA AGTCTTCCTG AGCCTTCCAT GTATCCTCAA TGCCCGGGGA 
TTAACCAGCG TTATCAACCA GAAGCTAAAG GATGATGAGG TTGCTCAGCT CAAGAAAAGT 
GCAGATACCC TGTGGGACAT CCAGAAGGAC CTAAAAGACC TGTGACTAGT GAGCTCTAGG 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LDHB ( XM_006719074.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006719137.1 Homo sapiens lactate dehydrogenase B (LDHB), transcript variant X1, mRNA.

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CloneID	OHu46104
Clone ID Related Accession (Same CDS sequence)	XM_006719074.2
Accession Version	XM_006719074.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	L-lactate dehydrogenase B chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006719074.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAACTC TTAAGGAAAA ACTCATTGCA CCAGTTGCGG AAGAAGAGGC AACAGTTCCA 
AACAATAAGA TCACTGTAGT GGGTGTTGGA CAAGTTGGTA TGGCGTGTGC TATCAGCATT 
CTGGGAAAGT CTCTGGCTGA TGAACTTGCT CTTGTGGATG TTTTGGAAGA TAAGCTTAAA 
GGAGAAATGA TGGATCTGCA GCATGGGAGC TTATTTCTTC AGACACCTAA AATTGTGGCA 
GATAAAGATT ATTCTGTGAC TGCCAATTCT AAGATTGTAG TGGTAACTGC AGGAGTCCGT 
CAGCAAGAAG GGGAGAGTCG GCTCAATCTG GTGCAGAGAA ATGTTAATGT CTTCAAATTC 
ATTATTCCTC AGATCGTCAA GTACAGTCCT GATTGCATCA TAATTGTGGT TTCCAACCCA 
GTGGACATTC TTACGTATGT TACCTGGAAA CTAAGTGGAT TACCCAAACA CCGCGTGATT 
GGAAGTGGAT GTAATCTGGA TTCTGCTAGA TTTCGCTACC TTATGGCTGA AAAACTTGGC 
ATTCATCCCA GCAGCTGCCA TGGATGGATT TTGGGGGAAC ATGGCGACTC AAGTGTGGCT 
GTGTGGAGTG GTGTGAATGT GGCAGGTGTT TCTCTCCAGG AATTGAATCC AGAAATGGGA 
ACTGACAATG ATAGTGAAAA TTGGAAGGAA GTGCATAAGA TGGTGGTTGA AAGTGCCTAT 
GAAGTCATCA AGCTAAAAGG ATATACCAAC TGGGCTATTG GATTAAGTGT GGCTGATCTT 
ATTGAATCCA TGTTGAAAAA TCTATCCAGG ATTCATCCCG TGTCAACAAT GGTAAAGGTA 
AGACAAACTA CCACAAAATG TAATATGATT TCTCGCATTG TATTCTACAA AAAGATTCAT 
TTAAAGAGGC TCAGACCAGA TAAAATCATA GTTCAAGGTT ATTTCTGTCT CTCAAAGTTT 
TATGTAAACT ATTCTCATTT TCCAACAGAA GTCTTGGACA GCAAGGGCAG AAAACTTAGA 
GAATCTAAGA CATTCAATAG ATTGTTGTCA TTCTTTATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006719137.1
CDS	228..1289
Translation	MATLKEKLIAPVAEEEATVPNNKITVVGVGQVGMACAISILGKSLADELALVDVLEDKLKGEMMDLQHGSLFLQTPKIVADKDYSVTANSKIVVVTAGVRQQEGESRLNLVQRNVNVFKFIIPQIVKYSPDCIIIVVSNPVDILTYVTWKLSGLPKHRVIGSGCNLDSARFRYLMAEKLGIHPSSCHGWILGEHGDSSVAVWSGVNVAGVSLQELNPEMGTDNDSENWKEVHKMVVESAYEVIKLKGYTNWAIGLSVADLIESMLKNLSRIHPVSTMVKVRQTTTKCNMISRIVFYKKIHLKRLRPDKIIVQGYFCLSKFYVNYSHFPTEVLDSKGRKLRESKTFNRLLSFFI

Target ORF information:

RefSeq Version	XM_006719074.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lactate dehydrogenase B (LDHB), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006719074.2

ORF Insert Sequence:

ATGGCAACTC TTAAGGAAAA ACTCATTGCA CCAGTTGCGG AAGAAGAGGC AACAGTTCCA 
AACAATAAGA TCACTGTAGT GGGTGTTGGA CAAGTTGGTA TGGCGTGTGC TATCAGCATT 
CTGGGAAAGT CTCTGGCTGA TGAACTTGCT CTTGTGGATG TTTTGGAAGA TAAGCTTAAA 
GGAGAAATGA TGGATCTGCA GCATGGGAGC TTATTTCTTC AGACACCTAA AATTGTGGCA 
GATAAAGATT ATTCTGTGAC TGCCAATTCT AAGATTGTAG TGGTAACTGC AGGAGTCCGT 
CAGCAAGAAG GGGAGAGTCG GCTCAATCTG GTGCAGAGAA ATGTTAATGT CTTCAAATTC 
ATTATTCCTC AGATCGTCAA GTACAGTCCT GATTGCATCA TAATTGTGGT TTCCAACCCA 
GTGGACATTC TTACGTATGT TACCTGGAAA CTAAGTGGAT TACCCAAACA CCGCGTGATT 
GGAAGTGGAT GTAATCTGGA TTCTGCTAGA TTTCGCTACC TTATGGCTGA AAAACTTGGC 
ATTCATCCCA GCAGCTGCCA TGGATGGATT TTGGGGGAAC ATGGCGACTC AAGTGTGGCT 
GTGTGGAGTG GTGTGAATGT GGCAGGTGTT TCTCTCCAGG AATTGAATCC AGAAATGGGA 
ACTGACAATG ATAGTGAAAA TTGGAAGGAA GTGCATAAGA TGGTGGTTGA AAGTGCCTAT 
GAAGTCATCA AGCTAAAAGG ATATACCAAC TGGGCTATTG GATTAAGTGT GGCTGATCTT 
ATTGAATCCA TGTTGAAAAA TCTATCCAGG ATTCATCCCG TGTCAACAAT GGTAAAGGTA 
AGACAAACTA CCACAAAATG TAATATGATT TCTCGCATTG TATTCTACAA AAAGATTCAT 
TTAAAGAGGC TCAGACCAGA TAAAATCATA GTTCAAGGTT ATTTCTGTCT CTCAAAGTTT 
TATGTAAACT ATTCTCATTT TCCAACAGAA GTCTTGGACA GCAAGGGCAG AAAACTTAGA 
GAATCTAAGA CATTCAATAG ATTGTTGTCA TTCTTTATAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ribosomal readthrough at a short UGA stop codon context triggers dual localization of metabolic enzymes in Fungi and animals.
PLoS genetics10(10)e1004685(2014 Oct)
Stiebler AC,Freitag J,Schink KO,Stehlik T,Tillmann BA,Ast J,B?lker M

Peroxisomal lactate dehydrogenase is generated by translational readthrough in mammals.
eLife3e03640(2014 Sep)
Schueren F,Lingner T,George R,Hofhuis J,Dickel C,G?rtner J,Thoms S

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LDHB cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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