LDHB cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	LDHB
Entrez Gene ID	281275
Full Name	lactate dehydrogenase B
General protein information	Preferred Names lactate dehydrogenase B Names L-lactate dehydrogenase B chain LDH-B
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Summary	This gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Recent studies have shown that two protein isoforms are produced from the same mRNA by use of alternative in-frame translation termination codons via a stop codon readthrough mechanism, and that these C-terminally distinct isoforms have different subcellular localization. Pseudogenes have been identified on chromosomes 1 and 11. [provided by RefSeq, Oct 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_174100.2	NP_776525.2	L-lactate dehydrogenase B chain isoform LDHB
NM_001316338.1	NP_001303267.1	L-lactate dehydrogenase B chain isoform LDHBx

Pathways from BioSystems

Homologies

Homo sapiens (human)	LDHB	NP_002291.1
Bos taurus (cattle)	LDHB	NP_776525.1
Danio rerio (zebrafish)	ldhbb	NP_001002474.1
Danio rerio (zebrafish)	ldhba	NP_571322.1
Xenopus tropicalis (tropical clawed frog)	ldhb	NP_988882.1
Pan troglodytes (chimpanzee)	LDHB	NP_001092031.1
	LDHB	XP_001094453.2
Canis lupus familiaris (dog)	LDHB	NP_001239093.1
Rattus norvegicus (Norway rat)	Ldhb	NP_036727.1
Gallus gallus (chicken)	LDHB	NP_989508.1
Mus musculus (house mouse)	Ldhb	NP_032518.1

Gene Ontology
Bibliography

Related articles in PubMed

Ribosomal readthrough at a short UGA stop codon context triggers dual localization of metabolic enzymes in Fungi and animals.
Stiebler AC, Freitag J, Schink KO, Stehlik T, Tillmann BA, Ast J, B?lker M
PLoS genetics10(10)e1004685(2014 Oct)

Peroxisomal lactate dehydrogenase is generated by translational readthrough in mammals.
Schueren F, Lingner T, George R, Hofhuis J, Dickel C, G?rtner J, Thoms S
eLife3e03640(2014 Sep)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Characterization of gene expression profiles in early bovine pregnancy using a custom cDNA microarray.
Ishiwata H, Katsuma S, Kizaki K, Patel OV, Nakano H, Takahashi T, Imai K, Hirasawa A, Shiojima S, Ikawa H, Suzuki Y, Tsujimoto G, Izaike Y, Todoroki J, Hashizume K
Molecular reproduction and development65(1)9-18(2003 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LDHB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LDHB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa95420	NM_174100.2 Latest version!	Bos taurus lactate dehydrogenase B (LDHB), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OBa95421	NM_001316338.1 Latest version!	Bos taurus lactate dehydrogenase B (LDHB), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LDHB ( NM_174100.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776525.2 Bos taurus lactate dehydrogenase B (LDHB), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OBa95420
Clone ID Related Accession (Same CDS sequence)	NM_174100.2
Accession Version	NM_174100.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1005bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Bos taurus(cattle)
Product	L-lactate dehydrogenase B chain isoform LDHB
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC142006.1, DT720873.1 and EE338507.1. On Oct 14, 2015 this sequence version replaced NM_174100.1. Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (LDHB) results from translation termination at the upstream UGA stop codon, while the longer isoform (LDHBx) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the shorter isoform (LDHB), which is localized in the cytosol. ##Evidence-Data-START## Transcript exon combination :: BC142006.1, DV884659.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAACTC TTAAGGAAAA ATTGATTGCA CCAGTTGCAG AAGAAGAGAC AAGGATCCCA 
AACAATAAGA TCACTGTAGT GGGTGTTGGA CAAGTTGGTA TGGCATGTGC CATCAGCATT 
CTGGGAAAGT CTCTGACTGA CGAGCTTGCT CTTGTGGATG TTTTGGAAGA TAAACTCAAA 
GGAGAAATGA TGGACCTGCA GCACGGGAGC TTATTCCTTC AGACACCAAA AATTGTGGCA 
GACAAAGATT ACTCTGTCAC TGCCAATTCC AAGATTGTGG TGGTAACTGC AGGAGTTCGC 
CAGCAAGAAG GGGAGAGTCG CCTGAATTTG GTGCAAAGGA ACGTTAACGT CTTCAAGTTC 
ATCATTCCTC AGATCGTCAA GTACAGTCCT GACTGCATCA TCATTGTGGT TTCCAACCCA 
GTGGATATTC TCACATATGT TACCTGGAAA CTAAGTGGAT TACCCAAGCA CCGTGTGATT 
GGGAGTGGAT GTAACCTGGA TTCTGCTAGA TTTCGCTACC TTATGGCTGA AAAACTTGGC 
ATTCATCCCA GCAGCTGCCA CGGATGGATT TTGGGGGAAC ATGGCGACTC AAGCGTGGCT 
GTGTGGAGTG GAGTGAATGT GGCAGGCGTT TCTCTCCAGG AACTGAATCC AGAAATGGGA 
ACAGACAATG ATAGTGAAAA TTGGAAGGAA GTGCATAAGA TGGTGGTTGA GAGTGCCTAT 
GAAGTCATCA AGCTAAAAGG ATATACCAAC TGGGCTATTG GATTAAGTGT GGCTGATCTT 
ATTGAATCCA TGTTGAAAAA TCTATCGAGG ATTCACCCAG TGTCAACAAT GGTGAAGGGC 
ATGTATGGCA TTGAGAATGA AGTCTTCCTG AGCCTTCCGT GTATCCTGAA TGCTCGAGGG 
TTAACCAGTG TTATCAACCA GAAGCTGAAG GATGAAGAGG TTGCTCAACT CAAGAAAAGT 
GCAGACACCC TCTGGGGCAT CCAAAAGGAC CTGAAGGACC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776525.2
CDS	145..1149
Translation	MATLKEKLIAPVAEEETRIPNNKITVVGVGQVGMACAISILGKSLTDELALVDVLEDKLKGEMMDLQHGSLFLQTPKIVADKDYSVTANSKIVVVTAGVRQQEGESRLNLVQRNVNVFKFIIPQIVKYSPDCIIIVVSNPVDILTYVTWKLSGLPKHRVIGSGCNLDSARFRYLMAEKLGIHPSSCHGWILGEHGDSSVAVWSGVNVAGVSLQELNPEMGTDNDSENWKEVHKMVVESAYEVIKLKGYTNWAIGLSVADLIESMLKNLSRIHPVSTMVKGMYGIENEVFLSLPCILNARGLTSVINQKLKDEEVAQLKKSADTLWGIQKDLKDL

Target ORF information:

RefSeq Version	NM_174100.2
Organism	Bos taurus(cattle)
Definition	Bos taurus lactate dehydrogenase B (LDHB), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174100.2

ORF Insert Sequence:

ATGGCAACTC TTAAGGAAAA ATTGATTGCA CCAGTTGCAG AAGAAGAGAC AAGGATCCCA 
AACAATAAGA TCACTGTAGT GGGTGTTGGA CAAGTTGGTA TGGCATGTGC CATCAGCATT 
CTGGGAAAGT CTCTGACTGA CGAGCTTGCT CTTGTGGATG TTTTGGAAGA TAAACTCAAA 
GGAGAAATGA TGGACCTGCA GCACGGGAGC TTATTCCTTC AGACACCAAA AATTGTGGCA 
GACAAAGATT ACTCTGTCAC TGCCAATTCC AAGATTGTGG TGGTAACTGC AGGAGTTCGC 
CAGCAAGAAG GGGAGAGTCG CCTGAATTTG GTGCAAAGGA ACGTTAACGT CTTCAAGTTC 
ATCATTCCTC AGATCGTCAA GTACAGTCCT GACTGCATCA TCATTGTGGT TTCCAACCCA 
GTGGATATTC TCACATATGT TACCTGGAAA CTAAGTGGAT TACCCAAGCA CCGTGTGATT 
GGGAGTGGAT GTAACCTGGA TTCTGCTAGA TTTCGCTACC TTATGGCTGA AAAACTTGGC 
ATTCATCCCA GCAGCTGCCA CGGATGGATT TTGGGGGAAC ATGGCGACTC AAGCGTGGCT 
GTGTGGAGTG GAGTGAATGT GGCAGGCGTT TCTCTCCAGG AACTGAATCC AGAAATGGGA 
ACAGACAATG ATAGTGAAAA TTGGAAGGAA GTGCATAAGA TGGTGGTTGA GAGTGCCTAT 
GAAGTCATCA AGCTAAAAGG ATATACCAAC TGGGCTATTG GATTAAGTGT GGCTGATCTT 
ATTGAATCCA TGTTGAAAAA TCTATCGAGG ATTCACCCAG TGTCAACAAT GGTGAAGGGC 
ATGTATGGCA TTGAGAATGA AGTCTTCCTG AGCCTTCCGT GTATCCTGAA TGCTCGAGGG 
TTAACCAGTG TTATCAACCA GAAGCTGAAG GATGAAGAGG TTGCTCAACT CAAGAAAAGT 
GCAGACACCC TCTGGGGCAT CCAAAAGGAC CTGAAGGACC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LDHB ( NM_001316338.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001303267.1 Bos taurus lactate dehydrogenase B (LDHB), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OBa95421
Clone ID Related Accession (Same CDS sequence)	NM_001316338.1
Accession Version	NM_001316338.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Bos taurus(cattle)
Product	L-lactate dehydrogenase B chain isoform LDHBx
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC142006.1, DT720873.1 and EE338507.1. Transcript Variant: This variant (1) encodes two isoforms, which result from the use of alternative in-frame translation termination codons. The shorter isoform (LDHB) results from translation termination at the upstream UGA stop codon, while the longer isoform (LDHBx) results from UGA stop codon readthrough to the downstream UAG termination codon. This RefSeq represents the longer, C-terminally extended isoform (LDHBx), which is localized in the peroxisomes. As the UGA stop codon has been reported to specify several alternative amino acids (tryptophan, cysteine, arginine and serine), its location in the longer isoform is denoted by an 'X'. ##Evidence-Data-START## Transcript exon combination :: BC142006.1, DV884659.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## stop codon readthrough :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAACTC TTAAGGAAAA ATTGATTGCA CCAGTTGCAG AAGAAGAGAC AAGGATCCCA 
AACAATAAGA TCACTGTAGT GGGTGTTGGA CAAGTTGGTA TGGCATGTGC CATCAGCATT 
CTGGGAAAGT CTCTGACTGA CGAGCTTGCT CTTGTGGATG TTTTGGAAGA TAAACTCAAA 
GGAGAAATGA TGGACCTGCA GCACGGGAGC TTATTCCTTC AGACACCAAA AATTGTGGCA 
GACAAAGATT ACTCTGTCAC TGCCAATTCC AAGATTGTGG TGGTAACTGC AGGAGTTCGC 
CAGCAAGAAG GGGAGAGTCG CCTGAATTTG GTGCAAAGGA ACGTTAACGT CTTCAAGTTC 
ATCATTCCTC AGATCGTCAA GTACAGTCCT GACTGCATCA TCATTGTGGT TTCCAACCCA 
GTGGATATTC TCACATATGT TACCTGGAAA CTAAGTGGAT TACCCAAGCA CCGTGTGATT 
GGGAGTGGAT GTAACCTGGA TTCTGCTAGA TTTCGCTACC TTATGGCTGA AAAACTTGGC 
ATTCATCCCA GCAGCTGCCA CGGATGGATT TTGGGGGAAC ATGGCGACTC AAGCGTGGCT 
GTGTGGAGTG GAGTGAATGT GGCAGGCGTT TCTCTCCAGG AACTGAATCC AGAAATGGGA 
ACAGACAATG ATAGTGAAAA TTGGAAGGAA GTGCATAAGA TGGTGGTTGA GAGTGCCTAT 
GAAGTCATCA AGCTAAAAGG ATATACCAAC TGGGCTATTG GATTAAGTGT GGCTGATCTT 
ATTGAATCCA TGTTGAAAAA TCTATCGAGG ATTCACCCAG TGTCAACAAT GGTGAAGGGC 
ATGTATGGCA TTGAGAATGA AGTCTTCCTG AGCCTTCCGT GTATCCTGAA TGCTCGAGGG 
TTAACCAGTG TTATCAACCA GAAGCTGAAG GATGAAGAGG TTGCTCAACT CAAGAAAAGT 
GCAGACACCC TCTGGGGCAT CCAAAAGGAC CTGAAGGACC TGTGACTTCC GGCTGCAAGG 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303267.1
CDS	145..1170
Translation	MATLKEKLIAPVAEEETRIPNNKITVVGVGQVGMACAISILGKSLTDELALVDVLEDKLKGEMMDLQHGSLFLQTPKIVADKDYSVTANSKIVVVTAGVRQQEGESRLNLVQRNVNVFKFIIPQIVKYSPDCIIIVVSNPVDILTYVTWKLSGLPKHRVIGSGCNLDSARFRYLMAEKLGIHPSSCHGWILGEHGDSSVAVWSGVNVAGVSLQELNPEMGTDNDSENWKEVHKMVVESAYEVIKLKGYTNWAIGLSVADLIESMLKNLSRIHPVSTMVKGMYGIENEVFLSLPCILNARGLTSVINQKLKDEEVAQLKKSADTLWGIQKDLKDL*LPAARL

Target ORF information:

RefSeq Version	NM_001316338.1
Organism	Bos taurus(cattle)
Definition	Bos taurus lactate dehydrogenase B (LDHB), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316338.1

ORF Insert Sequence:

ATGGCAACTC TTAAGGAAAA ATTGATTGCA CCAGTTGCAG AAGAAGAGAC AAGGATCCCA 
AACAATAAGA TCACTGTAGT GGGTGTTGGA CAAGTTGGTA TGGCATGTGC CATCAGCATT 
CTGGGAAAGT CTCTGACTGA CGAGCTTGCT CTTGTGGATG TTTTGGAAGA TAAACTCAAA 
GGAGAAATGA TGGACCTGCA GCACGGGAGC TTATTCCTTC AGACACCAAA AATTGTGGCA 
GACAAAGATT ACTCTGTCAC TGCCAATTCC AAGATTGTGG TGGTAACTGC AGGAGTTCGC 
CAGCAAGAAG GGGAGAGTCG CCTGAATTTG GTGCAAAGGA ACGTTAACGT CTTCAAGTTC 
ATCATTCCTC AGATCGTCAA GTACAGTCCT GACTGCATCA TCATTGTGGT TTCCAACCCA 
GTGGATATTC TCACATATGT TACCTGGAAA CTAAGTGGAT TACCCAAGCA CCGTGTGATT 
GGGAGTGGAT GTAACCTGGA TTCTGCTAGA TTTCGCTACC TTATGGCTGA AAAACTTGGC 
ATTCATCCCA GCAGCTGCCA CGGATGGATT TTGGGGGAAC ATGGCGACTC AAGCGTGGCT 
GTGTGGAGTG GAGTGAATGT GGCAGGCGTT TCTCTCCAGG AACTGAATCC AGAAATGGGA 
ACAGACAATG ATAGTGAAAA TTGGAAGGAA GTGCATAAGA TGGTGGTTGA GAGTGCCTAT 
GAAGTCATCA AGCTAAAAGG ATATACCAAC TGGGCTATTG GATTAAGTGT GGCTGATCTT 
ATTGAATCCA TGTTGAAAAA TCTATCGAGG ATTCACCCAG TGTCAACAAT GGTGAAGGGC 
ATGTATGGCA TTGAGAATGA AGTCTTCCTG AGCCTTCCGT GTATCCTGAA TGCTCGAGGG 
TTAACCAGTG TTATCAACCA GAAGCTGAAG GATGAAGAGG TTGCTCAACT CAAGAAAAGT 
GCAGACACCC TCTGGGGCAT CCAAAAGGAC CTGAAGGACC TGTGACTTCC GGCTGCAAGG 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ribosomal readthrough at a short UGA stop codon context triggers dual localization of metabolic enzymes in Fungi and animals.
PLoS genetics10(10)e1004685(2014 Oct)
Stiebler AC,Freitag J,Schink KO,Stehlik T,Tillmann BA,Ast J,B?lker M

Peroxisomal lactate dehydrogenase is generated by translational readthrough in mammals.
eLife3e03640(2014 Sep)
Schueren F,Lingner T,George R,Hofhuis J,Dickel C,G?rtner J,Thoms S

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Characterization of gene expression profiles in early bovine pregnancy using a custom cDNA microarray.
Molecular reproduction and development65(1)9-18(2003 May)
Ishiwata H,Katsuma S,Kizaki K,Patel OV,Nakano H,Takahashi T,Imai K,Hirasawa A,Shiojima S,Ikawa H,Suzuki Y,Tsujimoto G,Izaike Y,Todoroki J,Hashizume K

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LDHB cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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