Itk cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Itk
Entrez Gene ID	16428
Full Name	IL2 inducible T cell kinase
Synonyms	Emt,Tcsk,Tsk
General protein information	Preferred Names IL2 inducible T cell kinase Names tyrosine-protein kinase ITK/TSK T-cell-specific kinase interleukin-2-inducible T cell kinase kinase TLK
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11 B1.1\|11 27.75 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001281965.1	NP_001268894.1	tyrosine-protein kinase ITK/TSK isoform 1
NM_010583.3	NP_034713.2	tyrosine-protein kinase ITK/TSK isoform 2
NM_001281966.1	NP_001268895.1	tyrosine-protein kinase ITK/TSK isoform 3
NM_001281968.1	NP_001268897.1	tyrosine-protein kinase ITK/TSK isoform 5
NM_001281967.1	NP_001268896.1	tyrosine-protein kinase ITK/TSK isoform 4

Pathways from BioSystems

Homologies

Homo sapiens (human)	ITK	NP_005537.3
Pan troglodytes (chimpanzee)	ITK	XP_001136073.1
Bos taurus (cattle)	ITK	NP_001098858.1
Canis lupus familiaris (dog)	ITK	XP_546277.3
Mus musculus (house mouse)	Itk	NP_001268894.1
	ITK	XP_001113468.1
Danio rerio (zebrafish)	itk	NP_571179.1
Rattus norvegicus (Norway rat)	Itk	NP_001102295.1
Gallus gallus (chicken)	ITK	XP_414568.3

Gene Ontology
Bibliography

Related articles in PubMed

ITK and RLK inhibitor PRN694 improves skin disease in two mouse models of psoriasis.
Fuhriman JM, Winge MCG, Haberstock-Debic H, Funk JO, Bradshaw JM, Marinkovich MP
The Journal of investigative dermatology(2017 Nov)

An Autoinhibitory Role for the Pleckstrin Homology Domain of Interleukin-2-Inducible Tyrosine Kinase and Its Interplay with Canonical Phospholipid Recognition.
Devkota S, Joseph RE, Boyken SE, Fulton DB, Andreotti AH
Biochemistry56(23)2938-2949(2017 06)

ITK signalling via the Ras/IRF4 pathway regulates the development and function of Tr1 cells.
Huang W, Solouki S, Koylass N, Zheng SG, August A
Nature communications815871(2017 Jun)

T-Bet independent development of IFN? secreting natural T helper 1 cell population in the absence of Itk.
Kannan AK, Mohinta S, Huang W, Huang L, Koylass N, Appleton JA, August A
Scientific reports745935(2017 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that the pleckstrin homology domain of Itk functions as a phospholipid bilayer recognition site that localizes Itk to membrane via phosphatidylinositol 3,4,5-trisphosphate (PIP3) binding; this specific binding inhibits the phospho-transfer reaction via an allosteric mechanism.
Title: An Autoinhibitory Role for the Pleckstrin Homology Domain of Interleukin-2-Inducible Tyrosine Kinase and Its Interplay with Canonical Phospholipid Recognition.

Our results provide new insight into the effect of ITK and suboptimal T-cell receptor signaling on CD8(+) T cell function, and how these may contribute to phenotypes associated with ITK deficiency
Title: Inducible T Cell Kinase Regulates the Acquisition of Cytolytic Capacity and Degranulation in CD8

ITK and BTK regulate thermal homeostasis during septic response through mast cell function in mice.
Title: Nonreceptor tyrosine kinases ITK and BTK negatively regulate mast cell proinflammatory responses to lipopolysaccharide.

pharmacological inhibition of ITK resulted in T cell hyperplasia and the increased production of TH2-type cytokines.
Title: Inhibition of the kinase ITK in a mouse model of asthma reduces cell death and fails to inhibit the inflammatory response.

Taken together, these data indicate that ITK plus RLK inhibition may have therapeutic potential in Th1-mediated inflammatory diseases.
Title: A Small Molecule Inhibitor of ITK and RLK Impairs Th1 Differentiation and Prevents Colitis Disease Progression.

The following Itk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Itk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu20336	NM_001281966.1 Latest version!	Mus musculus IL2 inducible T cell kinase (Itk), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu20399	NM_001281968.1 Latest version!	Mus musculus IL2 inducible T cell kinase (Itk), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu20400	NM_001281965.1 Latest version!	Mus musculus IL2 inducible T cell kinase (Itk), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu20483	NM_001281967.1 Latest version!	Mus musculus IL2 inducible T cell kinase (Itk), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu20589	NM_010583.3 Latest version!	Mus musculus IL2 inducible T cell kinase (Itk), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Itk ( NM_001281966.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001268895.1 Mus musculus IL2 inducible T cell kinase (Itk), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu20336
Clone ID Related Accession (Same CDS sequence)	NM_001281966.1
Accession Version	NM_001281966.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1869bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	tyrosine-protein kinase ITK/TSK isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB843988.1, D14042.1, AK153613.1 and BY589043.1. Transcript Variant: This variant (3) uses two alternate in-frame splice sites in the central coding region, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK153613.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACAACT TCATCCTCCT GGAAGAACAG CTGATCAAGA AGTCCCAACA GAAGAGAAGG 
ACTTCTCCCT CGAATTTTAA AGTTCGTTTC TTTGTCTTAA CGAAAGCCAG CCTGGCCTAC 
TTTGAGGACC GCCACGGGAA GAAGCGCACG TTGAAGGGCT CCATTGAACT CTCCAGAATC 
AAGTGTGTGG AGATTGTCAA GAGTGACATT AGCATCCCGT GCCACTATAA ATACCCTTTT 
CAGGTCGTGC ATGACAACTA TCTCCTGTAT GTGTTTGCTC CAGACTGTGA GAGTCGGCAG 
CGCTGGGTGC TGACCCTTAA AGAAGAAACG AGGAATAACA ACAGCCTGGT ATCCAAGTAT 
CACCCTAATT TCTGGATGGA TGGGCGGTGG AGGTGCTGCT CCCAGCTGGA GAAGCCTGCT 
GTAGGCTGTG CTCCCTACGA CCCATCCAAG AATGCTTCAA AGAAGCCTCT TCCTCCTACT 
CCTGAAGACA ACAGGGTAAG TGAGCGGTCA TTTCAGGAAC CTGAAGAAAC CCTGGTCATT 
GCCTTGTACG ACTACCAAAC CAACGACCCT CAGGAGCTCG CACTGCGGTG TGATGAAGAG 
TACTACCTGC TGGACAGCTC CGAGATCCAC TGGTGGAGGG TTCAAGACAA AAATGGGCAT 
GAAGGATATG CACCAAGCAG TTACCTGGTA GAAAAATCTC CAAATAACCT TGAAACCTAT 
GAGTGGTACA ATAAAAGCAT CAGCCGCGAC AAAGCTGAAA AACTTCTTTT GGACACAGGT 
AAAGAAGGAG CTTTCATGGT CCGAGATTCC AGGACACCCG GGACATACAC AGTCTCTGTT 
TTCACCAAGG CCATCATAAG TGAGAACCCC TGTATAAAAC ATTATCACAT CAAAGAAACA 
AATGACAGCC CCAAGCGCTA CTACGTGGCT GAGAAGTATG TGTTTGACTC CATCCCTCTC 
CTCATCCAGT ATCACCAGTA CAATGGAGGA GGTTTGGTCA CTCGACTCCG CTATCCAGTT 
TGCTCCTGGA GACAAAAAGC CCCTGTCACA GCAGGGCTAA GATATGGGAA GTGGGTGATC 
CAACCCTCAG AGCTAACGTT CGTGCAGGAG ATTGGCAGCG GGCAGTTTGG GCTGGTGCAT 
CTCGGCTACT GGCTCAACAA GGACAAGGTG GCCATCAAGA CCATTCAGGA AGGGGCGATG 
TCAGAAGAAG ACTTTATCGA GGAGGCGGAA GTCATGATGA AACTCTCTCA CCCCAAACTG 
GTGCAGCTCT ATGGGGTGTG CCTGGAGCAA GCCCCCATCT GCCTGGTGTT TGAGTTCATG 
GAGCACGGCT GCCTGTCGGA TTACCTTCGA AGTCAGCGGG GTCTCTTTGC TGCGGAGACC 
CTGCTGGGCA TGTGCCTGGA TGTGTGTGAG GGCATGGCCT ACCTGGAAAA AGCTTGTGTC 
ATCCACAGAG ACCTGGCAGC CAGAAACTGT TTGGTGGGAG AAAACCAGGT CATCAAGGTG 
TCCGACTTTG GGATGACAAG ATTTGTCCTT GATGATCAAT ATACCAGCTC CACGGGCACC 
AAATTCCCAG TGAAGTGGGC ATCCCCAGAG GTGTTCTCCT TTAGTCGCTA TAGCAGCAAG 
TCAGATGTGT GGTCGTTTGG TGTACTGATG TGGGAAGTCT TCAGTGAAGG CAAAATCCCA 
TACGAAAACC GAAGCAATTC CGAGGTCGTG GAAGATATCA GCACTGGCTT TCGGTTATAC 
AAGCCCCGCC TGGCCTCCTG TCATGTCTAC CAGATCATGA ATCATTGCTG GAAAGAGAAA 
CCAGAAGACC GGCCACCCTT CTCCCAGCTG TTAAGCCAGC TGGCTGAAAT CGCAGAAGCT 
GGGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268895.1
CDS	106..1974
Translation	MNNFILLEEQLIKKSQQKRRTSPSNFKVRFFVLTKASLAYFEDRHGKKRTLKGSIELSRIKCVEIVKSDISIPCHYKYPFQVVHDNYLLYVFAPDCESRQRWVLTLKEETRNNNSLVSKYHPNFWMDGRWRCCSQLEKPAVGCAPYDPSKNASKKPLPPTPEDNRVSERSFQEPEETLVIALYDYQTNDPQELALRCDEEYYLLDSSEIHWWRVQDKNGHEGYAPSSYLVEKSPNNLETYEWYNKSISRDKAEKLLLDTGKEGAFMVRDSRTPGTYTVSVFTKAIISENPCIKHYHIKETNDSPKRYYVAEKYVFDSIPLLIQYHQYNGGGLVTRLRYPVCSWRQKAPVTAGLRYGKWVIQPSELTFVQEIGSGQFGLVHLGYWLNKDKVAIKTIQEGAMSEEDFIEEAEVMMKLSHPKLVQLYGVCLEQAPICLVFEFMEHGCLSDYLRSQRGLFAAETLLGMCLDVCEGMAYLEKACVIHRDLAARNCLVGENQVIKVSDFGMTRFVLDDQYTSSTGTKFPVKWASPEVFSFSRYSSKSDVWSFGVLMWEVFSEGKIPYENRSNSEVVEDISTGFRLYKPRLASCHVYQIMNHCWKEKPEDRPPFSQLLSQLAEIAEAGL

Target ORF information:

RefSeq Version	NM_001281966.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus IL2 inducible T cell kinase (Itk), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001281966.1

ORF Insert Sequence:

ATGAACAACT TCATCCTCCT GGAAGAACAG CTGATCAAGA AGTCCCAACA GAAGAGAAGG 
ACTTCTCCCT CGAATTTTAA AGTTCGTTTC TTTGTCTTAA CGAAAGCCAG CCTGGCCTAC 
TTTGAGGACC GCCACGGGAA GAAGCGCACG TTGAAGGGCT CCATTGAACT CTCCAGAATC 
AAGTGTGTGG AGATTGTCAA GAGTGACATT AGCATCCCGT GCCACTATAA ATACCCTTTT 
CAGGTCGTGC ATGACAACTA TCTCCTGTAT GTGTTTGCTC CAGACTGTGA GAGTCGGCAG 
CGCTGGGTGC TGACCCTTAA AGAAGAAACG AGGAATAACA ACAGCCTGGT ATCCAAGTAT 
CACCCTAATT TCTGGATGGA TGGGCGGTGG AGGTGCTGCT CCCAGCTGGA GAAGCCTGCT 
GTAGGCTGTG CTCCCTACGA CCCATCCAAG AATGCTTCAA AGAAGCCTCT TCCTCCTACT 
CCTGAAGACA ACAGGGTAAG TGAGCGGTCA TTTCAGGAAC CTGAAGAAAC CCTGGTCATT 
GCCTTGTACG ACTACCAAAC CAACGACCCT CAGGAGCTCG CACTGCGGTG TGATGAAGAG 
TACTACCTGC TGGACAGCTC CGAGATCCAC TGGTGGAGGG TTCAAGACAA AAATGGGCAT 
GAAGGATATG CACCAAGCAG TTACCTGGTA GAAAAATCTC CAAATAACCT TGAAACCTAT 
GAGTGGTACA ATAAAAGCAT CAGCCGCGAC AAAGCTGAAA AACTTCTTTT GGACACAGGT 
AAAGAAGGAG CTTTCATGGT CCGAGATTCC AGGACACCCG GGACATACAC AGTCTCTGTT 
TTCACCAAGG CCATCATAAG TGAGAACCCC TGTATAAAAC ATTATCACAT CAAAGAAACA 
AATGACAGCC CCAAGCGCTA CTACGTGGCT GAGAAGTATG TGTTTGACTC CATCCCTCTC 
CTCATCCAGT ATCACCAGTA CAATGGAGGA GGTTTGGTCA CTCGACTCCG CTATCCAGTT 
TGCTCCTGGA GACAAAAAGC CCCTGTCACA GCAGGGCTAA GATATGGGAA GTGGGTGATC 
CAACCCTCAG AGCTAACGTT CGTGCAGGAG ATTGGCAGCG GGCAGTTTGG GCTGGTGCAT 
CTCGGCTACT GGCTCAACAA GGACAAGGTG GCCATCAAGA CCATTCAGGA AGGGGCGATG 
TCAGAAGAAG ACTTTATCGA GGAGGCGGAA GTCATGATGA AACTCTCTCA CCCCAAACTG 
GTGCAGCTCT ATGGGGTGTG CCTGGAGCAA GCCCCCATCT GCCTGGTGTT TGAGTTCATG 
GAGCACGGCT GCCTGTCGGA TTACCTTCGA AGTCAGCGGG GTCTCTTTGC TGCGGAGACC 
CTGCTGGGCA TGTGCCTGGA TGTGTGTGAG GGCATGGCCT ACCTGGAAAA AGCTTGTGTC 
ATCCACAGAG ACCTGGCAGC CAGAAACTGT TTGGTGGGAG AAAACCAGGT CATCAAGGTG 
TCCGACTTTG GGATGACAAG ATTTGTCCTT GATGATCAAT ATACCAGCTC CACGGGCACC 
AAATTCCCAG TGAAGTGGGC ATCCCCAGAG GTGTTCTCCT TTAGTCGCTA TAGCAGCAAG 
TCAGATGTGT GGTCGTTTGG TGTACTGATG TGGGAAGTCT TCAGTGAAGG CAAAATCCCA 
TACGAAAACC GAAGCAATTC CGAGGTCGTG GAAGATATCA GCACTGGCTT TCGGTTATAC 
AAGCCCCGCC TGGCCTCCTG TCATGTCTAC CAGATCATGA ATCATTGCTG GAAAGAGAAA 
CCAGAAGACC GGCCACCCTT CTCCCAGCTG TTAAGCCAGC TGGCTGAAAT CGCAGAAGCT 
GGGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itk ( NM_001281968.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001268897.1 Mus musculus IL2 inducible T cell kinase (Itk), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OMu20399
Clone ID Related Accession (Same CDS sequence)	NM_001281968.1
Accession Version	NM_001281968.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	tyrosine-protein kinase ITK/TSK isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB843988.1, D14042.1, AK153613.1 and BY589043.1. Transcript Variant: This variant (5) uses two alternate in-frame splice sites in the central coding region, compared to variant 1. The encoded isoform (5) is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC128375.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACAACT TCATCCTCCT GGAAGAACAG CTGATCAAGA AGTCCCAACA GAAGAGAAGG 
ACTTCTCCCT CGAATTTTAA AGTTCGTTTC TTTGTCTTAA CGAAAGCCAG CCTGGCCTAC 
TTTGAGGACC GCCACGGGAA GCGCACGTTG AAGGGCTCCA TTGAACTCTC CAGAATCAAG 
TGTGTGGAGA TTGTCAAGAG TGACATTAGC ATCCCGTGCC ACTATAAATA CCCTTTTCAG 
GTCGTGCATG ACAACTATCT CCTGTATGTG TTTGCTCCAG ACTGTGAGAG TCGGCAGCGC 
TGGGTGCTGA CCCTTAAAGA AGAAACGAGG AATAACAACA GCCTGGTATC CAAGTATCAC 
CCTAATTTCT GGATGGATGG GCGGTGGAGG TGCTGCTCCC AGCTGGAGAA GCCTGCTGTA 
GGCTGTGCTC CCTACGACCC ATCCAAGAAT GCTTCAAAGA AGCCTCTTCC TCCTACTCCT 
GAAGACAACA GGCGGTCATT TCAGGAACCT GAAGAAACCC TGGTCATTGC CTTGTACGAC 
TACCAAACCA ACGACCCTCA GGAGCTCGCA CTGCGGTGTG ATGAAGAGTA CTACCTGCTG 
GACAGCTCCG AGATCCACTG GTGGAGGGTT CAAGACAAAA ATGGGCATGA AGGATATGCA 
CCAAGCAGTT ACCTGGTAGA AAAATCTCCA AATAACCTTG AAACCTATGA GTGGTACAAT 
AAAAGCATCA GCCGCGACAA AGCTGAAAAA CTTCTTTTGG ACACAGGTAA AGAAGGAGCT 
TTCATGGTCC GAGATTCCAG GACACCCGGG ACATACACAG TCTCTGTTTT CACCAAGGCC 
ATCATAAGTG AGAACCCCTG TATAAAACAT TATCACATCA AAGAAACAAA TGACAGCCCC 
AAGCGCTACT ACGTGGCTGA GAAGTATGTG TTTGACTCCA TCCCTCTCCT CATCCAGTAT 
CACCAGTACA ATGGAGGAGG TTTGGTCACT CGACTCCGCT ATCCAGTTTG CTCCTGGAGA 
CAAAAAGCCC CTGTCACAGC AGGGCTAAGA TATGGGAAGT GGGTGATCCA ACCCTCAGAG 
CTAACGTTCG TGCAGGAGAT TGGCAGCGGG CAGTTTGGGC TGGTGCATCT CGGCTACTGG 
CTCAACAAGG ACAAGGTGGC CATCAAGACC ATTCAGGAAG GGGCGATGTC AGAAGAAGAC 
TTTATCGAGG AGGCGGAAGT CATGATGAAA CTCTCTCACC CCAAACTGGT GCAGCTCTAT 
GGGGTGTGCC TGGAGCAAGC CCCCATCTGC CTGGTGTTTG AGTTCATGGA GCACGGCTGC 
CTGTCGGATT ACCTTCGAAG TCAGCGGGGT CTCTTTGCTG CGGAGACCCT GCTGGGCATG 
TGCCTGGATG TGTGTGAGGG CATGGCCTAC CTGGAAAAAG CTTGTGTCAT CCACAGAGAC 
CTGGCAGCCA GAAACTGTTT GGTGGGAGAA AACCAGGTCA TCAAGGTGTC CGACTTTGGG 
ATGACAAGAT TTGTCCTTGA TGATCAATAT ACCAGCTCCA CGGGCACCAA ATTCCCAGTG 
AAGTGGGCAT CCCCAGAGGT GTTCTCCTTT AGTCGCTATA GCAGCAAGTC AGATGTGTGG 
TCGTTTGGTG TACTGATGTG GGAAGTCTTC AGTGAAGGCA AAATCCCATA CGAAAACCGA 
AGCAATTCCG AGGTCGTGGA AGATATCAGC ACTGGCTTTC GGTTATACAA GCCCCGCCTG 
GCCTCCTGTC ATGTCTACCA GATCATGAAT CATTGCTGGA AAGAGAAACC AGAAGACCGG 
CCACCCTTCT CCCAGCTGTT AAGCCAGCTG GCTGAAATCG CAGAAGCTGG GCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268897.1
CDS	106..1962
Translation	MNNFILLEEQLIKKSQQKRRTSPSNFKVRFFVLTKASLAYFEDRHGKRTLKGSIELSRIKCVEIVKSDISIPCHYKYPFQVVHDNYLLYVFAPDCESRQRWVLTLKEETRNNNSLVSKYHPNFWMDGRWRCCSQLEKPAVGCAPYDPSKNASKKPLPPTPEDNRRSFQEPEETLVIALYDYQTNDPQELALRCDEEYYLLDSSEIHWWRVQDKNGHEGYAPSSYLVEKSPNNLETYEWYNKSISRDKAEKLLLDTGKEGAFMVRDSRTPGTYTVSVFTKAIISENPCIKHYHIKETNDSPKRYYVAEKYVFDSIPLLIQYHQYNGGGLVTRLRYPVCSWRQKAPVTAGLRYGKWVIQPSELTFVQEIGSGQFGLVHLGYWLNKDKVAIKTIQEGAMSEEDFIEEAEVMMKLSHPKLVQLYGVCLEQAPICLVFEFMEHGCLSDYLRSQRGLFAAETLLGMCLDVCEGMAYLEKACVIHRDLAARNCLVGENQVIKVSDFGMTRFVLDDQYTSSTGTKFPVKWASPEVFSFSRYSSKSDVWSFGVLMWEVFSEGKIPYENRSNSEVVEDISTGFRLYKPRLASCHVYQIMNHCWKEKPEDRPPFSQLLSQLAEIAEAGL

Target ORF information:

RefSeq Version	NM_001281968.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus IL2 inducible T cell kinase (Itk), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001281968.1

ORF Insert Sequence:

ATGAACAACT TCATCCTCCT GGAAGAACAG CTGATCAAGA AGTCCCAACA GAAGAGAAGG 
ACTTCTCCCT CGAATTTTAA AGTTCGTTTC TTTGTCTTAA CGAAAGCCAG CCTGGCCTAC 
TTTGAGGACC GCCACGGGAA GCGCACGTTG AAGGGCTCCA TTGAACTCTC CAGAATCAAG 
TGTGTGGAGA TTGTCAAGAG TGACATTAGC ATCCCGTGCC ACTATAAATA CCCTTTTCAG 
GTCGTGCATG ACAACTATCT CCTGTATGTG TTTGCTCCAG ACTGTGAGAG TCGGCAGCGC 
TGGGTGCTGA CCCTTAAAGA AGAAACGAGG AATAACAACA GCCTGGTATC CAAGTATCAC 
CCTAATTTCT GGATGGATGG GCGGTGGAGG TGCTGCTCCC AGCTGGAGAA GCCTGCTGTA 
GGCTGTGCTC CCTACGACCC ATCCAAGAAT GCTTCAAAGA AGCCTCTTCC TCCTACTCCT 
GAAGACAACA GGCGGTCATT TCAGGAACCT GAAGAAACCC TGGTCATTGC CTTGTACGAC 
TACCAAACCA ACGACCCTCA GGAGCTCGCA CTGCGGTGTG ATGAAGAGTA CTACCTGCTG 
GACAGCTCCG AGATCCACTG GTGGAGGGTT CAAGACAAAA ATGGGCATGA AGGATATGCA 
CCAAGCAGTT ACCTGGTAGA AAAATCTCCA AATAACCTTG AAACCTATGA GTGGTACAAT 
AAAAGCATCA GCCGCGACAA AGCTGAAAAA CTTCTTTTGG ACACAGGTAA AGAAGGAGCT 
TTCATGGTCC GAGATTCCAG GACACCCGGG ACATACACAG TCTCTGTTTT CACCAAGGCC 
ATCATAAGTG AGAACCCCTG TATAAAACAT TATCACATCA AAGAAACAAA TGACAGCCCC 
AAGCGCTACT ACGTGGCTGA GAAGTATGTG TTTGACTCCA TCCCTCTCCT CATCCAGTAT 
CACCAGTACA ATGGAGGAGG TTTGGTCACT CGACTCCGCT ATCCAGTTTG CTCCTGGAGA 
CAAAAAGCCC CTGTCACAGC AGGGCTAAGA TATGGGAAGT GGGTGATCCA ACCCTCAGAG 
CTAACGTTCG TGCAGGAGAT TGGCAGCGGG CAGTTTGGGC TGGTGCATCT CGGCTACTGG 
CTCAACAAGG ACAAGGTGGC CATCAAGACC ATTCAGGAAG GGGCGATGTC AGAAGAAGAC 
TTTATCGAGG AGGCGGAAGT CATGATGAAA CTCTCTCACC CCAAACTGGT GCAGCTCTAT 
GGGGTGTGCC TGGAGCAAGC CCCCATCTGC CTGGTGTTTG AGTTCATGGA GCACGGCTGC 
CTGTCGGATT ACCTTCGAAG TCAGCGGGGT CTCTTTGCTG CGGAGACCCT GCTGGGCATG 
TGCCTGGATG TGTGTGAGGG CATGGCCTAC CTGGAAAAAG CTTGTGTCAT CCACAGAGAC 
CTGGCAGCCA GAAACTGTTT GGTGGGAGAA AACCAGGTCA TCAAGGTGTC CGACTTTGGG 
ATGACAAGAT TTGTCCTTGA TGATCAATAT ACCAGCTCCA CGGGCACCAA ATTCCCAGTG 
AAGTGGGCAT CCCCAGAGGT GTTCTCCTTT AGTCGCTATA GCAGCAAGTC AGATGTGTGG 
TCGTTTGGTG TACTGATGTG GGAAGTCTTC AGTGAAGGCA AAATCCCATA CGAAAACCGA 
AGCAATTCCG AGGTCGTGGA AGATATCAGC ACTGGCTTTC GGTTATACAA GCCCCGCCTG 
GCCTCCTGTC ATGTCTACCA GATCATGAAT CATTGCTGGA AAGAGAAACC AGAAGACCGG 
CCACCCTTCT CCCAGCTGTT AAGCCAGCTG GCTGAAATCG CAGAAGCTGG GCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itk ( NM_001281965.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001268894.1 Mus musculus IL2 inducible T cell kinase (Itk), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu20400
Clone ID Related Accession (Same CDS sequence)	NM_001281965.1
Accession Version	NM_001281965.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	tyrosine-protein kinase ITK/TSK isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB843988.1, D14042.1, AL662806.20, AK153613.1 and BY589043.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L00619.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACAACT TCATCCTCCT GGAAGAACAG CTGATCAAGA AGTCCCAACA GAAGAGAAGG 
ACTTCTCCCT CGAATTTTAA AGTTCGTTTC TTTGTCTTAA CGAAAGCCAG CCTGGCCTAC 
TTTGAGGACC GCCACGGGAA GAAGCGCACG TTGAAGGGCT CCATTGAACT CTCCAGAATC 
AAGTGTGTGG AGATTGTCAA GAGTGACATT AGCATCCCGT GCCACTATAA ATACCCTTTT 
CAGACTCTTG TGTACTTACA GGTCGTGCAT GACAACTATC TCCTGTATGT GTTTGCTCCA 
GACTGTGAGA GTCGGCAGCG CTGGGTGCTG ACCCTTAAAG AAGAAACGAG GAATAACAAC 
AGCCTGGTAT CCAAGTATCA CCCTAATTTC TGGATGGATG GGCGGTGGAG GTGCTGCTCC 
CAGCTGGAGA AGCCTGCTGT AGGCTGTGCT CCCTACGACC CATCCAAGAA TGCTTCAAAG 
AAGCCTCTTC CTCCTACTCC TGAAGACAAC AGGCGGTCAT TTCAGGAACC TGAAGAAACC 
CTGGTCATTG CCTTGTACGA CTACCAAACC AACGACCCTC AGGAGCTCGC ACTGCGGTGT 
GATGAAGAGT ACTACCTGCT GGACAGCTCC GAGATCCACT GGTGGAGGGT TCAAGACAAA 
AATGGGCATG AAGGATATGC ACCAAGCAGT TACCTGGTAG AAAAATCTCC AAATAACCTT 
GAAACCTATG AGTGGTACAA TAAAAGCATC AGCCGCGACA AAGCTGAAAA ACTTCTTTTG 
GACACAGGTA AAGAAGGAGC TTTCATGGTC CGAGATTCCA GGACACCCGG GACATACACA 
GTCTCTGTTT TCACCAAGGC CATCATAAGT GAGAACCCCT GTATAAAACA TTATCACATC 
AAAGAAACAA ATGACAGCCC CAAGCGCTAC TACGTGGCTG AGAAGTATGT GTTTGACTCC 
ATCCCTCTCC TCATCCAGTA TCACCAGTAC AATGGAGGAG GTTTGGTCAC TCGACTCCGC 
TATCCAGTTT GCTCCTGGAG ACAAAAAGCC CCTGTCACAG CAGGGCTAAG ATATGGGAAG 
TGGGTGATCC AACCCTCAGA GCTAACGTTC GTGCAGGAGA TTGGCAGCGG GCAGTTTGGG 
CTGGTGCATC TCGGCTACTG GCTCAACAAG GACAAGGTGG CCATCAAGAC CATTCAGGAA 
GGGGCGATGT CAGAAGAAGA CTTTATCGAG GAGGCGGAAG TCATGATGAA ACTCTCTCAC 
CCCAAACTGG TGCAGCTCTA TGGGGTGTGC CTGGAGCAAG CCCCCATCTG CCTGGTGTTT 
GAGTTCATGG AGCACGGCTG CCTGTCGGAT TACCTTCGAA GTCAGCGGGG TCTCTTTGCT 
GCGGAGACCC TGCTGGGCAT GTGCCTGGAT GTGTGTGAGG GCATGGCCTA CCTGGAAAAA 
GCTTGTGTCA TCCACAGAGA CCTGGCAGCC AGAAACTGTT TGGTGGGAGA AAACCAGGTC 
ATCAAGGTGT CCGACTTTGG GATGACAAGA TTTGTCCTTG ATGATCAATA TACCAGCTCC 
ACGGGCACCA AATTCCCAGT GAAGTGGGCA TCCCCAGAGG TGTTCTCCTT TAGTCGCTAT 
AGCAGCAAGT CAGATGTGTG GTCGTTTGGT GTACTGATGT GGGAAGTCTT CAGTGAAGGC 
AAAATCCCAT ACGAAAACCG AAGCAATTCC GAGGTCGTGG AAGATATCAG CACTGGCTTT 
CGGTTATACA AGCCCCGCCT GGCCTCCTGT CATGTCTACC AGATCATGAA TCATTGCTGG 
AAAGAGAAAC CAGAAGACCG GCCACCCTTC TCCCAGCTGT TAAGCCAGCT GGCTGAAATC 
GCAGAAGCTG GGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268894.1
CDS	106..1983
Translation	MNNFILLEEQLIKKSQQKRRTSPSNFKVRFFVLTKASLAYFEDRHGKKRTLKGSIELSRIKCVEIVKSDISIPCHYKYPFQTLVYLQVVHDNYLLYVFAPDCESRQRWVLTLKEETRNNNSLVSKYHPNFWMDGRWRCCSQLEKPAVGCAPYDPSKNASKKPLPPTPEDNRRSFQEPEETLVIALYDYQTNDPQELALRCDEEYYLLDSSEIHWWRVQDKNGHEGYAPSSYLVEKSPNNLETYEWYNKSISRDKAEKLLLDTGKEGAFMVRDSRTPGTYTVSVFTKAIISENPCIKHYHIKETNDSPKRYYVAEKYVFDSIPLLIQYHQYNGGGLVTRLRYPVCSWRQKAPVTAGLRYGKWVIQPSELTFVQEIGSGQFGLVHLGYWLNKDKVAIKTIQEGAMSEEDFIEEAEVMMKLSHPKLVQLYGVCLEQAPICLVFEFMEHGCLSDYLRSQRGLFAAETLLGMCLDVCEGMAYLEKACVIHRDLAARNCLVGENQVIKVSDFGMTRFVLDDQYTSSTGTKFPVKWASPEVFSFSRYSSKSDVWSFGVLMWEVFSEGKIPYENRSNSEVVEDISTGFRLYKPRLASCHVYQIMNHCWKEKPEDRPPFSQLLSQLAEIAEAGL

Target ORF information:

RefSeq Version	NM_001281965.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus IL2 inducible T cell kinase (Itk), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001281965.1

ORF Insert Sequence:

ATGAACAACT TCATCCTCCT GGAAGAACAG CTGATCAAGA AGTCCCAACA GAAGAGAAGG 
ACTTCTCCCT CGAATTTTAA AGTTCGTTTC TTTGTCTTAA CGAAAGCCAG CCTGGCCTAC 
TTTGAGGACC GCCACGGGAA GAAGCGCACG TTGAAGGGCT CCATTGAACT CTCCAGAATC 
AAGTGTGTGG AGATTGTCAA GAGTGACATT AGCATCCCGT GCCACTATAA ATACCCTTTT 
CAGACTCTTG TGTACTTACA GGTCGTGCAT GACAACTATC TCCTGTATGT GTTTGCTCCA 
GACTGTGAGA GTCGGCAGCG CTGGGTGCTG ACCCTTAAAG AAGAAACGAG GAATAACAAC 
AGCCTGGTAT CCAAGTATCA CCCTAATTTC TGGATGGATG GGCGGTGGAG GTGCTGCTCC 
CAGCTGGAGA AGCCTGCTGT AGGCTGTGCT CCCTACGACC CATCCAAGAA TGCTTCAAAG 
AAGCCTCTTC CTCCTACTCC TGAAGACAAC AGGCGGTCAT TTCAGGAACC TGAAGAAACC 
CTGGTCATTG CCTTGTACGA CTACCAAACC AACGACCCTC AGGAGCTCGC ACTGCGGTGT 
GATGAAGAGT ACTACCTGCT GGACAGCTCC GAGATCCACT GGTGGAGGGT TCAAGACAAA 
AATGGGCATG AAGGATATGC ACCAAGCAGT TACCTGGTAG AAAAATCTCC AAATAACCTT 
GAAACCTATG AGTGGTACAA TAAAAGCATC AGCCGCGACA AAGCTGAAAA ACTTCTTTTG 
GACACAGGTA AAGAAGGAGC TTTCATGGTC CGAGATTCCA GGACACCCGG GACATACACA 
GTCTCTGTTT TCACCAAGGC CATCATAAGT GAGAACCCCT GTATAAAACA TTATCACATC 
AAAGAAACAA ATGACAGCCC CAAGCGCTAC TACGTGGCTG AGAAGTATGT GTTTGACTCC 
ATCCCTCTCC TCATCCAGTA TCACCAGTAC AATGGAGGAG GTTTGGTCAC TCGACTCCGC 
TATCCAGTTT GCTCCTGGAG ACAAAAAGCC CCTGTCACAG CAGGGCTAAG ATATGGGAAG 
TGGGTGATCC AACCCTCAGA GCTAACGTTC GTGCAGGAGA TTGGCAGCGG GCAGTTTGGG 
CTGGTGCATC TCGGCTACTG GCTCAACAAG GACAAGGTGG CCATCAAGAC CATTCAGGAA 
GGGGCGATGT CAGAAGAAGA CTTTATCGAG GAGGCGGAAG TCATGATGAA ACTCTCTCAC 
CCCAAACTGG TGCAGCTCTA TGGGGTGTGC CTGGAGCAAG CCCCCATCTG CCTGGTGTTT 
GAGTTCATGG AGCACGGCTG CCTGTCGGAT TACCTTCGAA GTCAGCGGGG TCTCTTTGCT 
GCGGAGACCC TGCTGGGCAT GTGCCTGGAT GTGTGTGAGG GCATGGCCTA CCTGGAAAAA 
GCTTGTGTCA TCCACAGAGA CCTGGCAGCC AGAAACTGTT TGGTGGGAGA AAACCAGGTC 
ATCAAGGTGT CCGACTTTGG GATGACAAGA TTTGTCCTTG ATGATCAATA TACCAGCTCC 
ACGGGCACCA AATTCCCAGT GAAGTGGGCA TCCCCAGAGG TGTTCTCCTT TAGTCGCTAT 
AGCAGCAAGT CAGATGTGTG GTCGTTTGGT GTACTGATGT GGGAAGTCTT CAGTGAAGGC 
AAAATCCCAT ACGAAAACCG AAGCAATTCC GAGGTCGTGG AAGATATCAG CACTGGCTTT 
CGGTTATACA AGCCCCGCCT GGCCTCCTGT CATGTCTACC AGATCATGAA TCATTGCTGG 
AAAGAGAAAC CAGAAGACCG GCCACCCTTC TCCCAGCTGT TAAGCCAGCT GGCTGAAATC 
GCAGAAGCTG GGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itk ( NM_001281967.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001268896.1 Mus musculus IL2 inducible T cell kinase (Itk), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu20483
Clone ID Related Accession (Same CDS sequence)	NM_001281967.1
Accession Version	NM_001281967.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	tyrosine-protein kinase ITK/TSK isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB843988.1 and BC028478.1. Transcript Variant: This variant (4) lacks several exons and its 3'-terminal exon extends past a splice site that is used in variant 1. The encoded isoform (4) has a shorter and distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC028478.1, AK079693.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACAACT TCATCCTCCT GGAAGAACAG CTGATCAAGA AGTCCCAACA GAAGAGAAGG 
ACTTCTCCCT CGAATTTTAA AGTTCGTTTC TTTGTCTTAA CGAAAGCCAG CCTGGCCTAC 
TTTGAGGACC GCCACGGGAA GAAGCGCACG TTGAAGGGCT CCATTGAACT CTCCAGAATC 
AAGTGTGTGG AGATTGTCAA GAGTGACATT AGCATCCCGT GCCACTATAA ATACCCTTTT 
CAGGTCGTGC ATGACAACTA TCTCCTGTAT GTGTTTGCTC CAGACTGTGA GAGTCGGCAG 
CGCTGGGTGC TGACCCTTAA AGAAGAAACG AGGAATAACA ACAGCCTGGT ATCCAAGTAT 
CACCCTAATT TCTGGATGGA TGGGCGGTGG AGGTGCTGCT CCCAGCTGGA GAAGCCTGCT 
GTAGGCTGTG CTCCCTACGA CCCATCCAAG AATGCTTCAA AGAAGCCTCT TCCTCCTACT 
CCTGAAGACA ACAGGCGGTC ATTTCAGGAA CCTGAAGAAA CCCTGGTCAT TGCCTTGTAC 
GACTACCAAA CCAACGACCC TCAGGAGCTC GCACTGCGGT GTGATGAAGA GTACTACCTG 
CTGGACAGCT CCGAGATCCA CTGGTGGAGG GTTCAAGACA AAAATGGGCA TGAAGGATAT 
GCACCAAGCA GTTACCTGGT AGAAAAATCT CCAAATAACC TTGAAACCTA TGAGTGGTAC 
AATAAAAGCA TCAGCCGCGA CAAAGCTGAA AAACTTCTTT TGGACACAGT GAAGAAATGG 
ACACGTATAC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001268896.1
CDS	106..900
Translation	MNNFILLEEQLIKKSQQKRRTSPSNFKVRFFVLTKASLAYFEDRHGKKRTLKGSIELSRIKCVEIVKSDISIPCHYKYPFQVVHDNYLLYVFAPDCESRQRWVLTLKEETRNNNSLVSKYHPNFWMDGRWRCCSQLEKPAVGCAPYDPSKNASKKPLPPTPEDNRRSFQEPEETLVIALYDYQTNDPQELALRCDEEYYLLDSSEIHWWRVQDKNGHEGYAPSSYLVEKSPNNLETYEWYNKSISRDKAEKLLLDTVKKWTRIL

Target ORF information:

RefSeq Version	NM_001281967.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus IL2 inducible T cell kinase (Itk), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001281967.1

ORF Insert Sequence:

ATGAACAACT TCATCCTCCT GGAAGAACAG CTGATCAAGA AGTCCCAACA GAAGAGAAGG 
ACTTCTCCCT CGAATTTTAA AGTTCGTTTC TTTGTCTTAA CGAAAGCCAG CCTGGCCTAC 
TTTGAGGACC GCCACGGGAA GAAGCGCACG TTGAAGGGCT CCATTGAACT CTCCAGAATC 
AAGTGTGTGG AGATTGTCAA GAGTGACATT AGCATCCCGT GCCACTATAA ATACCCTTTT 
CAGGTCGTGC ATGACAACTA TCTCCTGTAT GTGTTTGCTC CAGACTGTGA GAGTCGGCAG 
CGCTGGGTGC TGACCCTTAA AGAAGAAACG AGGAATAACA ACAGCCTGGT ATCCAAGTAT 
CACCCTAATT TCTGGATGGA TGGGCGGTGG AGGTGCTGCT CCCAGCTGGA GAAGCCTGCT 
GTAGGCTGTG CTCCCTACGA CCCATCCAAG AATGCTTCAA AGAAGCCTCT TCCTCCTACT 
CCTGAAGACA ACAGGCGGTC ATTTCAGGAA CCTGAAGAAA CCCTGGTCAT TGCCTTGTAC 
GACTACCAAA CCAACGACCC TCAGGAGCTC GCACTGCGGT GTGATGAAGA GTACTACCTG 
CTGGACAGCT CCGAGATCCA CTGGTGGAGG GTTCAAGACA AAAATGGGCA TGAAGGATAT 
GCACCAAGCA GTTACCTGGT AGAAAAATCT CCAAATAACC TTGAAACCTA TGAGTGGTAC 
AATAAAAGCA TCAGCCGCGA CAAAGCTGAA AAACTTCTTT TGGACACAGT GAAGAAATGG 
ACACGTATAC TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itk ( NM_010583.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034713.2 Mus musculus IL2 inducible T cell kinase (Itk), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu20589
Clone ID Related Accession (Same CDS sequence)	NM_010583.3
Accession Version	NM_010583.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	tyrosine-protein kinase ITK/TSK isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB843988.1, D14042.1, AK153613.1 and BY589043.1. On Aug 13, 2013 this sequence version replaced NM_010583.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (2) is shorter, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L05631.1, D14042.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAACAACT TCATCCTCCT GGAAGAACAG CTGATCAAGA AGTCCCAACA GAAGAGAAGG 
ACTTCTCCCT CGAATTTTAA AGTTCGTTTC TTTGTCTTAA CGAAAGCCAG CCTGGCCTAC 
TTTGAGGACC GCCACGGGAA GAAGCGCACG TTGAAGGGCT CCATTGAACT CTCCAGAATC 
AAGTGTGTGG AGATTGTCAA GAGTGACATT AGCATCCCGT GCCACTATAA ATACCCTTTT 
CAGGTCGTGC ATGACAACTA TCTCCTGTAT GTGTTTGCTC CAGACTGTGA GAGTCGGCAG 
CGCTGGGTGC TGACCCTTAA AGAAGAAACG AGGAATAACA ACAGCCTGGT ATCCAAGTAT 
CACCCTAATT TCTGGATGGA TGGGCGGTGG AGGTGCTGCT CCCAGCTGGA GAAGCCTGCT 
GTAGGCTGTG CTCCCTACGA CCCATCCAAG AATGCTTCAA AGAAGCCTCT TCCTCCTACT 
CCTGAAGACA ACAGGCGGTC ATTTCAGGAA CCTGAAGAAA CCCTGGTCAT TGCCTTGTAC 
GACTACCAAA CCAACGACCC TCAGGAGCTC GCACTGCGGT GTGATGAAGA GTACTACCTG 
CTGGACAGCT CCGAGATCCA CTGGTGGAGG GTTCAAGACA AAAATGGGCA TGAAGGATAT 
GCACCAAGCA GTTACCTGGT AGAAAAATCT CCAAATAACC TTGAAACCTA TGAGTGGTAC 
AATAAAAGCA TCAGCCGCGA CAAAGCTGAA AAACTTCTTT TGGACACAGG TAAAGAAGGA 
GCTTTCATGG TCCGAGATTC CAGGACACCC GGGACATACA CAGTCTCTGT TTTCACCAAG 
GCCATCATAA GTGAGAACCC CTGTATAAAA CATTATCACA TCAAAGAAAC AAATGACAGC 
CCCAAGCGCT ACTACGTGGC TGAGAAGTAT GTGTTTGACT CCATCCCTCT CCTCATCCAG 
TATCACCAGT ACAATGGAGG AGGTTTGGTC ACTCGACTCC GCTATCCAGT TTGCTCCTGG 
AGACAAAAAG CCCCTGTCAC AGCAGGGCTA AGATATGGGA AGTGGGTGAT CCAACCCTCA 
GAGCTAACGT TCGTGCAGGA GATTGGCAGC GGGCAGTTTG GGCTGGTGCA TCTCGGCTAC 
TGGCTCAACA AGGACAAGGT GGCCATCAAG ACCATTCAGG AAGGGGCGAT GTCAGAAGAA 
GACTTTATCG AGGAGGCGGA AGTCATGATG AAACTCTCTC ACCCCAAACT GGTGCAGCTC 
TATGGGGTGT GCCTGGAGCA AGCCCCCATC TGCCTGGTGT TTGAGTTCAT GGAGCACGGC 
TGCCTGTCGG ATTACCTTCG AAGTCAGCGG GGTCTCTTTG CTGCGGAGAC CCTGCTGGGC 
ATGTGCCTGG ATGTGTGTGA GGGCATGGCC TACCTGGAAA AAGCTTGTGT CATCCACAGA 
GACCTGGCAG CCAGAAACTG TTTGGTGGGA GAAAACCAGG TCATCAAGGT GTCCGACTTT 
GGGATGACAA GATTTGTCCT TGATGATCAA TATACCAGCT CCACGGGCAC CAAATTCCCA 
GTGAAGTGGG CATCCCCAGA GGTGTTCTCC TTTAGTCGCT ATAGCAGCAA GTCAGATGTG 
TGGTCGTTTG GTGTACTGAT GTGGGAAGTC TTCAGTGAAG GCAAAATCCC ATACGAAAAC 
CGAAGCAATT CCGAGGTCGT GGAAGATATC AGCACTGGCT TTCGGTTATA CAAGCCCCGC 
CTGGCCTCCT GTCATGTCTA CCAGATCATG AATCATTGCT GGAAAGAGAA ACCAGAAGAC 
CGGCCACCCT TCTCCCAGCT GTTAAGCCAG CTGGCTGAAA TCGCAGAAGC TGGGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034713.2
CDS	106..1965
Translation	MNNFILLEEQLIKKSQQKRRTSPSNFKVRFFVLTKASLAYFEDRHGKKRTLKGSIELSRIKCVEIVKSDISIPCHYKYPFQVVHDNYLLYVFAPDCESRQRWVLTLKEETRNNNSLVSKYHPNFWMDGRWRCCSQLEKPAVGCAPYDPSKNASKKPLPPTPEDNRRSFQEPEETLVIALYDYQTNDPQELALRCDEEYYLLDSSEIHWWRVQDKNGHEGYAPSSYLVEKSPNNLETYEWYNKSISRDKAEKLLLDTGKEGAFMVRDSRTPGTYTVSVFTKAIISENPCIKHYHIKETNDSPKRYYVAEKYVFDSIPLLIQYHQYNGGGLVTRLRYPVCSWRQKAPVTAGLRYGKWVIQPSELTFVQEIGSGQFGLVHLGYWLNKDKVAIKTIQEGAMSEEDFIEEAEVMMKLSHPKLVQLYGVCLEQAPICLVFEFMEHGCLSDYLRSQRGLFAAETLLGMCLDVCEGMAYLEKACVIHRDLAARNCLVGENQVIKVSDFGMTRFVLDDQYTSSTGTKFPVKWASPEVFSFSRYSSKSDVWSFGVLMWEVFSEGKIPYENRSNSEVVEDISTGFRLYKPRLASCHVYQIMNHCWKEKPEDRPPFSQLLSQLAEIAEAGL

Target ORF information:

RefSeq Version	NM_010583.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus IL2 inducible T cell kinase (Itk), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010583.3

ORF Insert Sequence:

ATGAACAACT TCATCCTCCT GGAAGAACAG CTGATCAAGA AGTCCCAACA GAAGAGAAGG 
ACTTCTCCCT CGAATTTTAA AGTTCGTTTC TTTGTCTTAA CGAAAGCCAG CCTGGCCTAC 
TTTGAGGACC GCCACGGGAA GAAGCGCACG TTGAAGGGCT CCATTGAACT CTCCAGAATC 
AAGTGTGTGG AGATTGTCAA GAGTGACATT AGCATCCCGT GCCACTATAA ATACCCTTTT 
CAGGTCGTGC ATGACAACTA TCTCCTGTAT GTGTTTGCTC CAGACTGTGA GAGTCGGCAG 
CGCTGGGTGC TGACCCTTAA AGAAGAAACG AGGAATAACA ACAGCCTGGT ATCCAAGTAT 
CACCCTAATT TCTGGATGGA TGGGCGGTGG AGGTGCTGCT CCCAGCTGGA GAAGCCTGCT 
GTAGGCTGTG CTCCCTACGA CCCATCCAAG AATGCTTCAA AGAAGCCTCT TCCTCCTACT 
CCTGAAGACA ACAGGCGGTC ATTTCAGGAA CCTGAAGAAA CCCTGGTCAT TGCCTTGTAC 
GACTACCAAA CCAACGACCC TCAGGAGCTC GCACTGCGGT GTGATGAAGA GTACTACCTG 
CTGGACAGCT CCGAGATCCA CTGGTGGAGG GTTCAAGACA AAAATGGGCA TGAAGGATAT 
GCACCAAGCA GTTACCTGGT AGAAAAATCT CCAAATAACC TTGAAACCTA TGAGTGGTAC 
AATAAAAGCA TCAGCCGCGA CAAAGCTGAA AAACTTCTTT TGGACACAGG TAAAGAAGGA 
GCTTTCATGG TCCGAGATTC CAGGACACCC GGGACATACA CAGTCTCTGT TTTCACCAAG 
GCCATCATAA GTGAGAACCC CTGTATAAAA CATTATCACA TCAAAGAAAC AAATGACAGC 
CCCAAGCGCT ACTACGTGGC TGAGAAGTAT GTGTTTGACT CCATCCCTCT CCTCATCCAG 
TATCACCAGT ACAATGGAGG AGGTTTGGTC ACTCGACTCC GCTATCCAGT TTGCTCCTGG 
AGACAAAAAG CCCCTGTCAC AGCAGGGCTA AGATATGGGA AGTGGGTGAT CCAACCCTCA 
GAGCTAACGT TCGTGCAGGA GATTGGCAGC GGGCAGTTTG GGCTGGTGCA TCTCGGCTAC 
TGGCTCAACA AGGACAAGGT GGCCATCAAG ACCATTCAGG AAGGGGCGAT GTCAGAAGAA 
GACTTTATCG AGGAGGCGGA AGTCATGATG AAACTCTCTC ACCCCAAACT GGTGCAGCTC 
TATGGGGTGT GCCTGGAGCA AGCCCCCATC TGCCTGGTGT TTGAGTTCAT GGAGCACGGC 
TGCCTGTCGG ATTACCTTCG AAGTCAGCGG GGTCTCTTTG CTGCGGAGAC CCTGCTGGGC 
ATGTGCCTGG ATGTGTGTGA GGGCATGGCC TACCTGGAAA AAGCTTGTGT CATCCACAGA 
GACCTGGCAG CCAGAAACTG TTTGGTGGGA GAAAACCAGG TCATCAAGGT GTCCGACTTT 
GGGATGACAA GATTTGTCCT TGATGATCAA TATACCAGCT CCACGGGCAC CAAATTCCCA 
GTGAAGTGGG CATCCCCAGA GGTGTTCTCC TTTAGTCGCT ATAGCAGCAA GTCAGATGTG 
TGGTCGTTTG GTGTACTGAT GTGGGAAGTC TTCAGTGAAG GCAAAATCCC ATACGAAAAC 
CGAAGCAATT CCGAGGTCGT GGAAGATATC AGCACTGGCT TTCGGTTATA CAAGCCCCGC 
CTGGCCTCCT GTCATGTCTA CCAGATCATG AATCATTGCT GGAAAGAGAA ACCAGAAGAC 
CGGCCACCCT TCTCCCAGCT GTTAAGCCAG CTGGCTGAAA TCGCAGAAGC TGGGCTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ITK and RLK inhibitor PRN694 improves skin disease in two mouse models of psoriasis.
The Journal of investigative dermatology(2017 Nov)
Fuhriman JM,Winge MCG,Haberstock-Debic H,Funk JO,Bradshaw JM,Marinkovich MP

An Autoinhibitory Role for the Pleckstrin Homology Domain of Interleukin-2-Inducible Tyrosine Kinase and Its Interplay with Canonical Phospholipid Recognition.
Biochemistry56(23)2938-2949(2017 06)
Devkota S,Joseph RE,Boyken SE,Fulton DB,Andreotti AH

ITK signalling via the Ras/IRF4 pathway regulates the development and function of Tr1 cells.
Nature communications815871(2017 Jun)
Huang W,Solouki S,Koylass N,Zheng SG,August A

T-Bet independent development of IFN? secreting natural T helper 1 cell population in the absence of Itk.
Scientific reports745935(2017 Apr)
Kannan AK,Mohinta S,Huang W,Huang L,Koylass N,Appleton JA,August A