Ihh cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ihh
Entrez Gene ID	16147
Full Name	Indian hedgehog
Synonyms	HHG-2
General protein information	Preferred Names Indian hedgehog Names indian hedgehog protein
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 C4\|1 38.55 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001313683.1	NP_001300612.1	indian hedgehog protein isoform 2 precursor
NM_010544.3	NP_034674.2	indian hedgehog protein isoform 1 preproprotein

Pathways from BioSystems

Homologies

Bos taurus (cattle)	IHH	NP_001070338.2
Danio rerio (zebrafish)	ihhb	NP_571163.1
Pan troglodytes (chimpanzee)	IHH	XP_526034.2
Rattus norvegicus (Norway rat)	Ihh	NP_445836.1
Xenopus tropicalis (tropical clawed frog)	ihh	XP_004917692.1
Homo sapiens (human)	IHH	NP_002172.2
	IHH	XP_001092385.2
Canis lupus familiaris (dog)	IHH	XP_545653.3
Mus musculus (house mouse)	Ihh	NP_034674.1
Gallus gallus (chicken)	IHH	NP_990288.1
Danio rerio (zebrafish)	ihha	NP_001030165.2

Gene Ontology

The following Ihh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ihh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu67991	NM_010544.3 Latest version!	Mus musculus Indian hedgehog (Ihh), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OMu67992	NM_001313683.1 Latest version!	Mus musculus Indian hedgehog (Ihh), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ihh ( NM_010544.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034674.2 Mus musculus Indian hedgehog (Ihh), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu67991
Clone ID Related Accession (Same CDS sequence)	NM_010544.3
Accession Version	NM_010544.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Mus musculus(house mouse)
Product	indian hedgehog protein isoform 1 preproprotein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC139023.15, AK144322.1 and AI323361.1. On Aug 25, 2015 this sequence version replaced NM_010544.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849377, SAMN00849380 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:9079674) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTCCCG CCTGGCTCCG GCCCCGACTG CGGTTCTGTC TGTTCCTGCT GCTGCTGCTT 
CTGGTGCCGG CGGCGCGGGG CTGCGGGCCG GGCCGGGTGG TGGGCAGCCG CCGGAGGCCG 
CCTCGCAAGC TCGTGCCTCT TGCCTACAAG CAGTTCAGCC CCAACGTGCC GGAGAAGACC 
CTGGGCGCCA GCGGGCGCTA CGAAGGCAAG ATCGCGCGCA GCTCTGAGCG CTTCAAAGAG 
CTCACCCCCA ACTACAATCC CGACATCATC TTCAAGGACG AGGAGAACAC GGGTGCCGAC 
CGCCTCATGA CCCAGCGCTG CAAGGACCGT CTGAACTCAC TGGCCATCTC TGTCATGAAC 
CAGTGGCCTG GTGTGAAACT GCGGGTGACC GAAGGCTGGG ATGAAGATGG CCATCACTCA 
GAGGAGTCTT TACACTATGA GGGCCGCGCG GTGGATATCA CCACCTCAGA CCGTGACCGA 
AATAAGTATG GACTGCTGGC GCGCTTAGCA GTGGAGGCCG GCTTCGACTG GGTGTATTAC 
GAGTCCAAGG CCCACGTGCA TTGCTCTGTC AAGTCTGAGC ATTCGGCCGC TGCCAAGACA 
GGTGGCTGCT TTCCTGCCGG AGCCCAGGTG CGCCTAGAGA ACGGGGAGCG TGTGGCCCTG 
TCAGCTGTAA AGCCAGGAGA CCGGGTGCTG GCCATGGGGG AGGATGGGAC CCCCACCTTC 
AGTGATGTGC TTATTTTCCT GGACCGCGAG CCAAACCGGC TGAGAGCTTT CCAGGTCATC 
GAGACTCAGG ATCCTCCGCG TCGGCTGGCG CTCACGCCTG CCCACCTGCT CTTCATTGCG 
GACAATCATA CAGAACCAGC AGCCCACTTC CGGGCCACAT TTGCCAGCCA TGTGCAACCA 
GGCCAATATG TGCTGGTATC AGGGGTACCA GGCCTCCAGC CTGCTCGGGT GGCAGCTGTC 
TCCACCCACG TGGCCCTTGG GTCCTATGCT CCTCTCACAA GGCATGGGAC ACTTGTGGTG 
GAGGATGTGG TGGCCTCCTG CTTTGCAGCT GTGGCTGACC ACCATCTGGC TCAGTTGGCC 
TTCTGGCCCC TGCGACTGTT TCCCAGTTTG GCATGGGGCA GCTGGACCCC AAGTGAGGGT 
GTTCACTGGT ACCCTCAGAT GCTCTACCGC CTGGGGCGTC TCTTGCTAGA AGAGAGCACC 
TTCCATCCAC TGGGCATGTC TGGGGCAGGA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034674.2
CDS	459..1694
Translation	MSPAWLRPRLRFCLFLLLLLLVPAARGCGPGRVVGSRRRPPRKLVPLAYKQFSPNVPEKTLGASGRYEGKIARSSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDRLNSLAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRNKYGLLARLAVEAGFDWVYYESKAHVHCSVKSEHSAAAKTGGCFPAGAQVRLENGERVALSAVKPGDRVLAMGEDGTPTFSDVLIFLDREPNRLRAFQVIETQDPPRRLALTPAHLLFIADNHTEPAAHFRATFASHVQPGQYVLVSGVPGLQPARVAAVSTHVALGSYAPLTRHGTLVVEDVVASCFAAVADHHLAQLAFWPLRLFPSLAWGSWTPSEGVHWYPQMLYRLGRLLLEESTFHPLGMSGAGS

Target ORF information:

RefSeq Version	NM_010544.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus Indian hedgehog (Ihh), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010544.3

ORF Insert Sequence:

ATGTCTCCCG CCTGGCTCCG GCCCCGACTG CGGTTCTGTC TGTTCCTGCT GCTGCTGCTT 
CTGGTGCCGG CGGCGCGGGG CTGCGGGCCG GGCCGGGTGG TGGGCAGCCG CCGGAGGCCG 
CCTCGCAAGC TCGTGCCTCT TGCCTACAAG CAGTTCAGCC CCAACGTGCC GGAGAAGACC 
CTGGGCGCCA GCGGGCGCTA CGAAGGCAAG ATCGCGCGCA GCTCTGAGCG CTTCAAAGAG 
CTCACCCCCA ACTACAATCC CGACATCATC TTCAAGGACG AGGAGAACAC GGGTGCCGAC 
CGCCTCATGA CCCAGCGCTG CAAGGACCGT CTGAACTCAC TGGCCATCTC TGTCATGAAC 
CAGTGGCCTG GTGTGAAACT GCGGGTGACC GAAGGCTGGG ATGAAGATGG CCATCACTCA 
GAGGAGTCTT TACACTATGA GGGCCGCGCG GTGGATATCA CCACCTCAGA CCGTGACCGA 
AATAAGTATG GACTGCTGGC GCGCTTAGCA GTGGAGGCCG GCTTCGACTG GGTGTATTAC 
GAGTCCAAGG CCCACGTGCA TTGCTCTGTC AAGTCTGAGC ATTCGGCCGC TGCCAAGACA 
GGTGGCTGCT TTCCTGCCGG AGCCCAGGTG CGCCTAGAGA ACGGGGAGCG TGTGGCCCTG 
TCAGCTGTAA AGCCAGGAGA CCGGGTGCTG GCCATGGGGG AGGATGGGAC CCCCACCTTC 
AGTGATGTGC TTATTTTCCT GGACCGCGAG CCAAACCGGC TGAGAGCTTT CCAGGTCATC 
GAGACTCAGG ATCCTCCGCG TCGGCTGGCG CTCACGCCTG CCCACCTGCT CTTCATTGCG 
GACAATCATA CAGAACCAGC AGCCCACTTC CGGGCCACAT TTGCCAGCCA TGTGCAACCA 
GGCCAATATG TGCTGGTATC AGGGGTACCA GGCCTCCAGC CTGCTCGGGT GGCAGCTGTC 
TCCACCCACG TGGCCCTTGG GTCCTATGCT CCTCTCACAA GGCATGGGAC ACTTGTGGTG 
GAGGATGTGG TGGCCTCCTG CTTTGCAGCT GTGGCTGACC ACCATCTGGC TCAGTTGGCC 
TTCTGGCCCC TGCGACTGTT TCCCAGTTTG GCATGGGGCA GCTGGACCCC AAGTGAGGGT 
GTTCACTGGT ACCCTCAGAT GCTCTACCGC CTGGGGCGTC TCTTGCTAGA AGAGAGCACC 
TTCCATCCAC TGGGCATGTC TGGGGCAGGA AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ihh ( NM_001313683.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001300612.1 Mus musculus Indian hedgehog (Ihh), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu67992
Clone ID Related Accession (Same CDS sequence)	NM_001313683.1
Accession Version	NM_001313683.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Mus musculus(house mouse)
Product	indian hedgehog protein isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC139023.15, AK144322.1 and AK090147.1. Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR compared to variant 1. The encoded isoform (2) has a distinct, shorter C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849389, SAMN01164137 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:9079674) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTCCCG CCTGGCTCCG GCCCCGACTG CGGTTCTGTC TGTTCCTGCT GCTGCTGCTT 
CTGGTGCCGG CGGCGCGGGG CTGCGGGCCG GGCCGGGTGG TGGGCAGCCG CCGGAGGCCG 
CCTCGCAAGC TCGTGCCTCT TGCCTACAAG CAGTTCAGCC CCAACGTGCC GGAGAAGACC 
CTGGGCGCCA GCGGGCGCTA CGAAGGCAAG ATCGCGCGCA GCTCTGAGCG CTTCAAAGAG 
CTCACCCCCA ACTACAATCC CGACATCATC TTCAAGGACG AGGAGAACAC GGGTGCCGAC 
CGCCTCATGA CCCAGCGCTG CAAGGACCGT CTGAACTCAC TGGCCATCTC TGTCATGAAC 
CAGTGGCCTG GTGTGAAACT GCGGGTGACC GAAGGCTGGG ATGAAGATGG CCATCACTCA 
GAGGAGTCTT TACACTATGA GGGCCGCGCG GTGGATATCA CCACCTCAGA CCGTGACCGA 
AATAAGTATG GACTGCTGGC GCGCTTAGCA GTGGAGGCCG GCTTCGACTG GGTGTATTAC 
GAGTCCAAGG CCCACGTGCA TTGCTCTGTC AAGTCTGGTG AGCCGCTGCT GCCGGGGGGC 
TGGGCCCGGG GCCGGGGCCG CAGGGAGTGG GCAGGCAGCG CAAAGCCCGC TAGGGCCAAG 
GAGAAGATGG CCTCCGGGGG ATGGAGGCGG GGACCCGAGG GAAGAGGATT TCCGAGGCGG 
CGGCGGCGGC GGCGGCGGCA GCGCTCCCTG CAGCTGCTTT GGACTGCTTG CTCCTCCCAT 
GTCCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300612.1
CDS	459..1247
Translation	MSPAWLRPRLRFCLFLLLLLLVPAARGCGPGRVVGSRRRPPRKLVPLAYKQFSPNVPEKTLGASGRYEGKIARSSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDRLNSLAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRNKYGLLARLAVEAGFDWVYYESKAHVHCSVKSGEPLLPGGWARGRGRREWAGSAKPARAKEKMASGGWRRGPEGRGFPRRRRRRRRQRSLQLLWTACSSHVR

Target ORF information:

RefSeq Version	NM_001313683.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus Indian hedgehog (Ihh), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313683.1

ORF Insert Sequence:

ATGTCTCCCG CCTGGCTCCG GCCCCGACTG CGGTTCTGTC TGTTCCTGCT GCTGCTGCTT 
CTGGTGCCGG CGGCGCGGGG CTGCGGGCCG GGCCGGGTGG TGGGCAGCCG CCGGAGGCCG 
CCTCGCAAGC TCGTGCCTCT TGCCTACAAG CAGTTCAGCC CCAACGTGCC GGAGAAGACC 
CTGGGCGCCA GCGGGCGCTA CGAAGGCAAG ATCGCGCGCA GCTCTGAGCG CTTCAAAGAG 
CTCACCCCCA ACTACAATCC CGACATCATC TTCAAGGACG AGGAGAACAC GGGTGCCGAC 
CGCCTCATGA CCCAGCGCTG CAAGGACCGT CTGAACTCAC TGGCCATCTC TGTCATGAAC 
CAGTGGCCTG GTGTGAAACT GCGGGTGACC GAAGGCTGGG ATGAAGATGG CCATCACTCA 
GAGGAGTCTT TACACTATGA GGGCCGCGCG GTGGATATCA CCACCTCAGA CCGTGACCGA 
AATAAGTATG GACTGCTGGC GCGCTTAGCA GTGGAGGCCG GCTTCGACTG GGTGTATTAC 
GAGTCCAAGG CCCACGTGCA TTGCTCTGTC AAGTCTGGTG AGCCGCTGCT GCCGGGGGGC 
TGGGCCCGGG GCCGGGGCCG CAGGGAGTGG GCAGGCAGCG CAAAGCCCGC TAGGGCCAAG 
GAGAAGATGG CCTCCGGGGG ATGGAGGCGG GGACCCGAGG GAAGAGGATT TCCGAGGCGG 
CGGCGGCGGC GGCGGCGGCA GCGCTCCCTG CAGCTGCTTT GGACTGCTTG CTCCTCCCAT 
GTCCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.