Fhl3 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fhl3
Entrez Gene ID	14201
Full Name	four and a half LIM domains 3
Synonyms	SLIM2
General protein information	Preferred Names four and a half LIM domains 3 Names four and a half LIM domains protein 3 skeletal muscle LIM-protein 2
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 D2.2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006502766.4	XP_006502829.1	four and a half LIM domains protein 3 isoform X1
XM_006502768.4	XP_006502831.1	four and a half LIM domains protein 3 isoform X2
XM_006502766.3	XP_006502829.1	four and a half LIM domains protein 3 isoform X1
XM_006502768.3	XP_006502831.1	four and a half LIM domains protein 3 isoform X2
XM_006502769.3	XP_006502832.1	four and a half LIM domains protein 3 isoform X3
XM_006502769.4	XP_006502832.1	four and a half LIM domains protein 3 isoform X3
NM_010213.3	NP_034343.2	four and a half LIM domains protein 3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FHL3	XP_003308038.1
Homo sapiens (human)	FHL3	NP_004459.2
	FHL3	XP_001112977.2
Rattus norvegicus (Norway rat)	Fhl3	NP_001101449.1
Danio rerio (zebrafish)	fhl3a	XP_695478.2
Xenopus tropicalis (tropical clawed frog)	fhl3	NP_001008165.1
Canis lupus familiaris (dog)	FHL3	XP_850071.2
Mus musculus (house mouse)	Fhl3	NP_034343.2
Gallus gallus (chicken)	FHL3	XP_004947806.1
Bos taurus (cattle)	FHL3	NP_001029395.1

Gene Ontology
Bibliography

Related articles in PubMed

FHL3 differentially regulates the expression of MyHC isoforms through interactions with MyoD and pCREB.
Zhang Y, Li W, Zhu M, Li Y, Xu Z, Zuo B
Cellular signalling28(1)60-73(2016 Jan)

In vivo and in vitro investigations of heterozygous nebulin knock-out mice disclose a mild skeletal muscle phenotype.
Gineste C, De Winter JM, Kohl C, Witt CC, Giannesini B, Brohm K, Le Fur Y, Gretz N, Vilmen C, Pecchi E, Jubeau M, Cozzone PJ, Stienen GJ, Granzier H, Labeit S, Ottenheijm CA, Bendahan D, Gondin J
Neuromuscular disorders : NMD23(4)357-69(2013 Apr)

Screening large numbers of expression patterns of transcription factors in late stages of the mouse thymus.
Chung YC, Tsai YJ, Shiu TY, Sun YY, Wang PF, Chen CL
Gene expression patterns : GEP11(1-2)84-92(2011 Jan-Feb)

Human four-and-a-half LIM family members suppress tumor cell growth through a TGF-beta-like signaling pathway.
Ding L, Wang Z, Yan J, Yang X, Liu A, Qiu W, Zhu J, Han J, Zhang H, Lin J, Cheng L, Qin X, Niu C, Yuan B, Wang X, Zhu C, Zhou Y, Li J, Song H, Huang C, Ye Q
The Journal of clinical investigation119(2)349-61(2009 Feb)

FHL3 binds MyoD and negatively regulates myotube formation.
Cottle DL, McGrath MJ, Cowling BS, Coghill ID, Brown S, Mitchell CA
Journal of cell science120(Pt 8)1423-35(2007 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Four and a half LIM domain protein 3 could interact with MyoD, cyclic AMP-responsive elements and phosphorylated cAMP response element binding protein in vivo
Title: FHL3 differentially regulates the expression of MyHC isoforms through interactions with MyoD and pCREB.

These studies demonstrate that Sox15, Fhl3 and Foxk1 function to coordinately regulate the myogenic progenitor cell population and skeletal muscle regeneration.
Title: Sox15 and Fhl3 transcriptionally coactivate Foxk1 and regulate myogenic progenitor cells.

FHL2 and FHL3, respectively, are colocalized with alpha(7)beta(1) integrin receptor at the periphery of Z-discs, suggesting a role in mechanical stabilization of muscle cells
Title: The LIM-only proteins FHL2 and FHL3 interact with alpha- and beta-subunits of the muscle alpha7beta1 integrin receptor.

Actin was identified as a FHL3 binding partner; FHL3 is a significant regulator of actin cytoskeletal dynamics in skeletal myoblasts.
Title: FHL3 is an actin-binding protein that regulates alpha-actinin-mediated actin bundling: FHL3 localizes to actin stress fibers and enhances cell spreading and stress fiber disassembly.

The following Fhl3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fhl3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15760	NM_010213.3 Latest version!	Mus musculus four and a half LIM domains 3 (Fhl3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu15760	XM_006502768.4 Latest version!	Mus musculus four and a half LIM domains 3 (Fhl3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu33354	XM_006502766.4 Latest version!	Mus musculus four and a half LIM domains 3 (Fhl3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu33356	XM_006502769.4 Latest version!	Mus musculus four and a half LIM domains 3 (Fhl3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu33354	XM_006502766.3	Mus musculus four and a half LIM domains 3 (Fhl3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu15760	XM_006502768.3	Mus musculus four and a half LIM domains 3 (Fhl3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OMu33356	XM_006502769.3	Mus musculus four and a half LIM domains 3 (Fhl3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fhl3 ( NM_010213.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_034343.2 Mus musculus four and a half LIM domains 3 (Fhl3), mRNA.

Price & Availability↑

CloneID	OMu15760
Clone ID Related Accession (Same CDS sequence)	XM_006502768.4 , XM_006502768.3 , NM_010213.3
Accession Version	NM_010213.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	four and a half LIM domains protein 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL606907.10. On Mar 17, 2010 this sequence version replaced NM_010213.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF114382.1, BC144903.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGAGG CATTTGACTG TGCAAAATGC AACGAGTCCT TGTACGGCCG CAAATACATC 
CAGACAGACA GTGGCCCCTA CTGCGTTCCG TGCTATGACA ACACCTTCGC CAACACCTGT 
GCTGAGTGCC AGCAGCTCAT TGGGCATGAT TCAAGGGAAC TGTTCTATGA GGACCGCCAC 
TTCCACGAGG GCTGCTTCCG CTGCTGCCGA TGCCAGCGCT CCCTTGCCGA TGAGCCCTTC 
ACCTGTCAGG ACAGTGAGCT TCTGTGTAAT GAGTGTTACT GCACAGCCTT CTCGTCCCAG 
TGTTCTGCCT GCGGGGAGAC TGTCATGCCT GGGTCCCGGA AGCTGGAGTA TGGAGGCCAG 
ACGTGGCATG AACACTGCTT TCTGTGCAGT GGCTGTGAGC AGCCGCTGGG CTCCCGCTCC 
TTCGTGCCTG ACAAGGGTGC CCACTACTGC GTGCCTTGCT ATGAGAACAA ATTTGCTCCT 
CGGTGTGCCC GCTGCAGCAA GACGCTGACC CAGGGTGGAG TGACATATCG TGATCAGCCC 
TGGCACCGAG AGTGCCTGGT CTGCACTGGG TGCAAGACGC CCCTTGCAGG GCAGCAGTTC 
ACATCTCGGG ATGATGATCC TTACTGTGTG GCCTGCTTTG GAGAACTCTT TGCACCCAAG 
TGCAGCAGCT GCAAGCGCCC CATCACAGGT GGGAGCGGCG GCGGCGAGGG CGCAGGACTC 
GGTGGAGGCA AGTATGTGTC CTTCGAAGAC CGACATTGGC ACCACAGCTG CTTTTCCTGT 
GCCCGCTGCT CCACCTCCCT GGTGGGCCAA GGCTTTGTAC CAGACGGAGA TCAAGTTCTA 
TGCCAGGGCT GCAGCCAAGC AGGCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_034343.2
CDS	175..1044
Translation	MSEAFDCAKCNESLYGRKYIQTDSGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNECYCTAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQGGVTYRDQPWHRECLVCTGCKTPLAGQQFTSRDDDPYCVACFGELFAPKCSSCKRPITGGSGGGEGAGLGGGKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGCSQAGP

Target ORF information:

RefSeq Version	NM_010213.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus four and a half LIM domains 3 (Fhl3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_010213.3

ORF Insert Sequence:

ATGAGCGAGG CATTTGACTG TGCAAAATGC AACGAGTCCT TGTACGGCCG CAAATACATC 
CAGACAGACA GTGGCCCCTA CTGCGTTCCG TGCTATGACA ACACCTTCGC CAACACCTGT 
GCTGAGTGCC AGCAGCTCAT TGGGCATGAT TCAAGGGAAC TGTTCTATGA GGACCGCCAC 
TTCCACGAGG GCTGCTTCCG CTGCTGCCGA TGCCAGCGCT CCCTTGCCGA TGAGCCCTTC 
ACCTGTCAGG ACAGTGAGCT TCTGTGTAAT GAGTGTTACT GCACAGCCTT CTCGTCCCAG 
TGTTCTGCCT GCGGGGAGAC TGTCATGCCT GGGTCCCGGA AGCTGGAGTA TGGAGGCCAG 
ACGTGGCATG AACACTGCTT TCTGTGCAGT GGCTGTGAGC AGCCGCTGGG CTCCCGCTCC 
TTCGTGCCTG ACAAGGGTGC CCACTACTGC GTGCCTTGCT ATGAGAACAA ATTTGCTCCT 
CGGTGTGCCC GCTGCAGCAA GACGCTGACC CAGGGTGGAG TGACATATCG TGATCAGCCC 
TGGCACCGAG AGTGCCTGGT CTGCACTGGG TGCAAGACGC CCCTTGCAGG GCAGCAGTTC 
ACATCTCGGG ATGATGATCC TTACTGTGTG GCCTGCTTTG GAGAACTCTT TGCACCCAAG 
TGCAGCAGCT GCAAGCGCCC CATCACAGGT GGGAGCGGCG GCGGCGAGGG CGCAGGACTC 
GGTGGAGGCA AGTATGTGTC CTTCGAAGAC CGACATTGGC ACCACAGCTG CTTTTCCTGT 
GCCCGCTGCT CCACCTCCCT GGTGGGCCAA GGCTTTGTAC CAGACGGAGA TCAAGTTCTA 
TGCCAGGGCT GCAGCCAAGC AGGCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fhl3 ( XM_006502768.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006502831.1 Mus musculus four and a half LIM domains 3 (Fhl3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu15760
Clone ID Related Accession (Same CDS sequence)	XM_006502768.4 , XM_006502768.3 , NM_010213.3
Accession Version	XM_006502768.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	four and a half LIM domains protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006502768.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGG CATTTGACTG TGCAAAATGC AACGAGTCCT TGTACGGCCG CAAATACATC 
CAGACAGACA GTGGCCCCTA CTGCGTTCCG TGCTATGACA ACACCTTCGC CAACACCTGT 
GCTGAGTGCC AGCAGCTCAT TGGGCATGAT TCAAGGGAAC TGTTCTATGA GGACCGCCAC 
TTCCACGAGG GCTGCTTCCG CTGCTGCCGA TGCCAGCGCT CCCTTGCCGA TGAGCCCTTC 
ACCTGTCAGG ACAGTGAGCT TCTGTGTAAT GAGTGTTACT GCACAGCCTT CTCGTCCCAG 
TGTTCTGCCT GCGGGGAGAC TGTCATGCCT GGGTCCCGGA AGCTGGAGTA TGGAGGCCAG 
ACGTGGCATG AACACTGCTT TCTGTGCAGT GGCTGTGAGC AGCCGCTGGG CTCCCGCTCC 
TTCGTGCCTG ACAAGGGTGC CCACTACTGC GTGCCTTGCT ATGAGAACAA ATTTGCTCCT 
CGGTGTGCCC GCTGCAGCAA GACGCTGACC CAGGGTGGAG TGACATATCG TGATCAGCCC 
TGGCACCGAG AGTGCCTGGT CTGCACTGGG TGCAAGACGC CCCTTGCAGG GCAGCAGTTC 
ACATCTCGGG ATGATGATCC TTACTGTGTG GCCTGCTTTG GAGAACTCTT TGCACCCAAG 
TGCAGCAGCT GCAAGCGCCC CATCACAGGT GGGAGCGGCG GCGGCGAGGG CGCAGGACTC 
GGTGGAGGCA AGTATGTGTC CTTCGAAGAC CGACATTGGC ACCACAGCTG CTTTTCCTGT 
GCCCGCTGCT CCACCTCCCT GGTGGGCCAA GGCTTTGTAC CAGACGGAGA TCAAGTTCTA 
TGCCAGGGCT GCAGCCAAGC AGGCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006502831.1
CDS	558..1427
Translation	MSEAFDCAKCNESLYGRKYIQTDSGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNECYCTAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQGGVTYRDQPWHRECLVCTGCKTPLAGQQFTSRDDDPYCVACFGELFAPKCSSCKRPITGGSGGGEGAGLGGGKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGCSQAGP

Target ORF information:

RefSeq Version	XM_006502768.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus four and a half LIM domains 3 (Fhl3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006502768.3

ORF Insert Sequence:

ATGAGCGAGG CATTTGACTG TGCAAAATGC AACGAGTCCT TGTACGGCCG CAAATACATC 
CAGACAGACA GTGGCCCCTA CTGCGTTCCG TGCTATGACA ACACCTTCGC CAACACCTGT 
GCTGAGTGCC AGCAGCTCAT TGGGCATGAT TCAAGGGAAC TGTTCTATGA GGACCGCCAC 
TTCCACGAGG GCTGCTTCCG CTGCTGCCGA TGCCAGCGCT CCCTTGCCGA TGAGCCCTTC 
ACCTGTCAGG ACAGTGAGCT TCTGTGTAAT GAGTGTTACT GCACAGCCTT CTCGTCCCAG 
TGTTCTGCCT GCGGGGAGAC TGTCATGCCT GGGTCCCGGA AGCTGGAGTA TGGAGGCCAG 
ACGTGGCATG AACACTGCTT TCTGTGCAGT GGCTGTGAGC AGCCGCTGGG CTCCCGCTCC 
TTCGTGCCTG ACAAGGGTGC CCACTACTGC GTGCCTTGCT ATGAGAACAA ATTTGCTCCT 
CGGTGTGCCC GCTGCAGCAA GACGCTGACC CAGGGTGGAG TGACATATCG TGATCAGCCC 
TGGCACCGAG AGTGCCTGGT CTGCACTGGG TGCAAGACGC CCCTTGCAGG GCAGCAGTTC 
ACATCTCGGG ATGATGATCC TTACTGTGTG GCCTGCTTTG GAGAACTCTT TGCACCCAAG 
TGCAGCAGCT GCAAGCGCCC CATCACAGGT GGGAGCGGCG GCGGCGAGGG CGCAGGACTC 
GGTGGAGGCA AGTATGTGTC CTTCGAAGAC CGACATTGGC ACCACAGCTG CTTTTCCTGT 
GCCCGCTGCT CCACCTCCCT GGTGGGCCAA GGCTTTGTAC CAGACGGAGA TCAAGTTCTA 
TGCCAGGGCT GCAGCCAAGC AGGCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fhl3 ( XM_006502766.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006502829.1 Mus musculus four and a half LIM domains 3 (Fhl3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu33354
Clone ID Related Accession (Same CDS sequence)	XM_006502766.4 , XM_006502766.3
Accession Version	XM_006502766.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	four and a half LIM domains protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006502766.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTTC TTGAGTTTGA GGGCTCACTG GGCACCGCCA CCATGAGCGA GGCATTTGAC 
TGTGCAAAAT GCAACGAGTC CTTGTACGGC CGCAAATACA TCCAGACAGA CAGTGGCCCC 
TACTGCGTTC CGTGCTATGA CAACACCTTC GCCAACACCT GTGCTGAGTG CCAGCAGCTC 
ATTGGGCATG ATTCAAGGGA ACTGTTCTAT GAGGACCGCC ACTTCCACGA GGGCTGCTTC 
CGCTGCTGCC GATGCCAGCG CTCCCTTGCC GATGAGCCCT TCACCTGTCA GGACAGTGAG 
CTTCTGTGTA ATGAGTGTTA CTGCACAGCC TTCTCGTCCC AGTGTTCTGC CTGCGGGGAG 
ACTGTCATGC CTGGGTCCCG GAAGCTGGAG TATGGAGGCC AGACGTGGCA TGAACACTGC 
TTTCTGTGCA GTGGCTGTGA GCAGCCGCTG GGCTCCCGCT CCTTCGTGCC TGACAAGGGT 
GCCCACTACT GCGTGCCTTG CTATGAGAAC AAATTTGCTC CTCGGTGTGC CCGCTGCAGC 
AAGACGCTGA CCCAGGGTGG AGTGACATAT CGTGATCAGC CCTGGCACCG AGAGTGCCTG 
GTCTGCACTG GGTGCAAGAC GCCCCTTGCA GGGCAGCAGT TCACATCTCG GGATGATGAT 
CCTTACTGTG TGGCCTGCTT TGGAGAACTC TTTGCACCCA AGTGCAGCAG CTGCAAGCGC 
CCCATCACAG GTGGGAGCGG CGGCGGCGAG GGCGCAGGAC TCGGTGGAGG CAAGTATGTG 
TCCTTCGAAG ACCGACATTG GCACCACAGC TGCTTTTCCT GTGCCCGCTG CTCCACCTCC 
CTGGTGGGCC AAGGCTTTGT ACCAGACGGA GATCAAGTTC TATGCCAGGG CTGCAGCCAA 
GCAGGCCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006502829.1
CDS	83..994
Translation	MGLLEFEGSLGTATMSEAFDCAKCNESLYGRKYIQTDSGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNECYCTAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQGGVTYRDQPWHRECLVCTGCKTPLAGQQFTSRDDDPYCVACFGELFAPKCSSCKRPITGGSGGGEGAGLGGGKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGCSQAGP

Target ORF information:

RefSeq Version	XM_006502766.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus four and a half LIM domains 3 (Fhl3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006502766.3

ORF Insert Sequence:

ATGGGGCTTC TTGAGTTTGA GGGCTCACTG GGCACCGCCA CCATGAGCGA GGCATTTGAC 
TGTGCAAAAT GCAACGAGTC CTTGTACGGC CGCAAATACA TCCAGACAGA CAGTGGCCCC 
TACTGCGTTC CGTGCTATGA CAACACCTTC GCCAACACCT GTGCTGAGTG CCAGCAGCTC 
ATTGGGCATG ATTCAAGGGA ACTGTTCTAT GAGGACCGCC ACTTCCACGA GGGCTGCTTC 
CGCTGCTGCC GATGCCAGCG CTCCCTTGCC GATGAGCCCT TCACCTGTCA GGACAGTGAG 
CTTCTGTGTA ATGAGTGTTA CTGCACAGCC TTCTCGTCCC AGTGTTCTGC CTGCGGGGAG 
ACTGTCATGC CTGGGTCCCG GAAGCTGGAG TATGGAGGCC AGACGTGGCA TGAACACTGC 
TTTCTGTGCA GTGGCTGTGA GCAGCCGCTG GGCTCCCGCT CCTTCGTGCC TGACAAGGGT 
GCCCACTACT GCGTGCCTTG CTATGAGAAC AAATTTGCTC CTCGGTGTGC CCGCTGCAGC 
AAGACGCTGA CCCAGGGTGG AGTGACATAT CGTGATCAGC CCTGGCACCG AGAGTGCCTG 
GTCTGCACTG GGTGCAAGAC GCCCCTTGCA GGGCAGCAGT TCACATCTCG GGATGATGAT 
CCTTACTGTG TGGCCTGCTT TGGAGAACTC TTTGCACCCA AGTGCAGCAG CTGCAAGCGC 
CCCATCACAG GTGGGAGCGG CGGCGGCGAG GGCGCAGGAC TCGGTGGAGG CAAGTATGTG 
TCCTTCGAAG ACCGACATTG GCACCACAGC TGCTTTTCCT GTGCCCGCTG CTCCACCTCC 
CTGGTGGGCC AAGGCTTTGT ACCAGACGGA GATCAAGTTC TATGCCAGGG CTGCAGCCAA 
GCAGGCCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fhl3 ( XM_006502769.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006502832.1 Mus musculus four and a half LIM domains 3 (Fhl3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu33356
Clone ID Related Accession (Same CDS sequence)	XM_006502769.3 , XM_006502769.4
Accession Version	XM_006502769.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	four and a half LIM domains protein 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006502769.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTTC TTGAGTTTGA GGGCTCACTG GGCACCGCCA CCATGAGCGA GGCATTTGAC 
TGTGCAAAAT GCAACGAGTC CTTGTACGGC CGCAAATACA TCCAGACAGA CAGTGGCCCC 
TACTGCGTTC CGTGCTATGA CAACACCTTC GCCAACACCT GTGCTGAGTG CCAGCAGCTC 
ATTGGGCATG ATTCAAGGGA ACTGTTCTAT GAGGACCGCC ACTTCCACGA GGGCTGCTTC 
CGCTGCTGCC GATGCCAGCG CTCCCTTGCC GATGAGCCCT TCACCTGTCA GGACAGTGAG 
CTTCTGTGTA ATGAGTGTTA CTGCACAGCC TTCTCGTCCC AGTGTTCTGC CTGCGGGGAG 
ACTGTCATGC CTGGGTCCCG GAAGCTGGAG TATGGAGGCC AGACGTGGCA TGAACACTGC 
TTTCTGTGCA GTGGCTGTGA GCAGCCGCTG GGCTCCCGCT CCTTCGTGCC TGACAAGGGT 
GCCCACTACT GCGTGCCTTG CTATGAGAAC AAATTTGCTC CTCGGTGTGC CCGCTGCAGC 
AAGGTTGGGG CTGGGCCTGG GAATTCAGGA CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006502832.1
CDS	86..661
Translation	MGLLEFEGSLGTATMSEAFDCAKCNESLYGRKYIQTDSGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNECYCTAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKVGAGPGNSGR

Target ORF information:

RefSeq Version	XM_006502769.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus four and a half LIM domains 3 (Fhl3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006502769.3

ORF Insert Sequence:

ATGGGGCTTC TTGAGTTTGA GGGCTCACTG GGCACCGCCA CCATGAGCGA GGCATTTGAC 
TGTGCAAAAT GCAACGAGTC CTTGTACGGC CGCAAATACA TCCAGACAGA CAGTGGCCCC 
TACTGCGTTC CGTGCTATGA CAACACCTTC GCCAACACCT GTGCTGAGTG CCAGCAGCTC 
ATTGGGCATG ATTCAAGGGA ACTGTTCTAT GAGGACCGCC ACTTCCACGA GGGCTGCTTC 
CGCTGCTGCC GATGCCAGCG CTCCCTTGCC GATGAGCCCT TCACCTGTCA GGACAGTGAG 
CTTCTGTGTA ATGAGTGTTA CTGCACAGCC TTCTCGTCCC AGTGTTCTGC CTGCGGGGAG 
ACTGTCATGC CTGGGTCCCG GAAGCTGGAG TATGGAGGCC AGACGTGGCA TGAACACTGC 
TTTCTGTGCA GTGGCTGTGA GCAGCCGCTG GGCTCCCGCT CCTTCGTGCC TGACAAGGGT 
GCCCACTACT GCGTGCCTTG CTATGAGAAC AAATTTGCTC CTCGGTGTGC CCGCTGCAGC 
AAGGTTGGGG CTGGGCCTGG GAATTCAGGA CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fhl3 ( XM_006502768.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006502831.1 Mus musculus four and a half LIM domains 3 (Fhl3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu15760
Clone ID Related Accession (Same CDS sequence)	XM_006502768.4 , XM_006502768.3 , NM_010213.3
Accession Version	XM_006502768.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 870bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	four and a half LIM domains protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006502768.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGG CATTTGACTG TGCAAAATGC AACGAGTCCT TGTACGGCCG CAAATACATC 
CAGACAGACA GTGGCCCCTA CTGCGTTCCG TGCTATGACA ACACCTTCGC CAACACCTGT 
GCTGAGTGCC AGCAGCTCAT TGGGCATGAT TCAAGGGAAC TGTTCTATGA GGACCGCCAC 
TTCCACGAGG GCTGCTTCCG CTGCTGCCGA TGCCAGCGCT CCCTTGCCGA TGAGCCCTTC 
ACCTGTCAGG ACAGTGAGCT TCTGTGTAAT GAGTGTTACT GCACAGCCTT CTCGTCCCAG 
TGTTCTGCCT GCGGGGAGAC TGTCATGCCT GGGTCCCGGA AGCTGGAGTA TGGAGGCCAG 
ACGTGGCATG AACACTGCTT TCTGTGCAGT GGCTGTGAGC AGCCGCTGGG CTCCCGCTCC 
TTCGTGCCTG ACAAGGGTGC CCACTACTGC GTGCCTTGCT ATGAGAACAA ATTTGCTCCT 
CGGTGTGCCC GCTGCAGCAA GACGCTGACC CAGGGTGGAG TGACATATCG TGATCAGCCC 
TGGCACCGAG AGTGCCTGGT CTGCACTGGG TGCAAGACGC CCCTTGCAGG GCAGCAGTTC 
ACATCTCGGG ATGATGATCC TTACTGTGTG GCCTGCTTTG GAGAACTCTT TGCACCCAAG 
TGCAGCAGCT GCAAGCGCCC CATCACAGGT GGGAGCGGCG GCGGCGAGGG CGCAGGACTC 
GGTGGAGGCA AGTATGTGTC CTTCGAAGAC CGACATTGGC ACCACAGCTG CTTTTCCTGT 
GCCCGCTGCT CCACCTCCCT GGTGGGCCAA GGCTTTGTAC CAGACGGAGA TCAAGTTCTA 
TGCCAGGGCT GCAGCCAAGC AGGCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006502831.1
CDS	557..1426
Translation	MSEAFDCAKCNESLYGRKYIQTDSGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNECYCTAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQGGVTYRDQPWHRECLVCTGCKTPLAGQQFTSRDDDPYCVACFGELFAPKCSSCKRPITGGSGGGEGAGLGGGKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGCSQAGP

Target ORF information:

RefSeq Version	XM_006502768.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus four and a half LIM domains 3 (Fhl3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006502768.4

ORF Insert Sequence:

ATGAGCGAGG CATTTGACTG TGCAAAATGC AACGAGTCCT TGTACGGCCG CAAATACATC 
CAGACAGACA GTGGCCCCTA CTGCGTTCCG TGCTATGACA ACACCTTCGC CAACACCTGT 
GCTGAGTGCC AGCAGCTCAT TGGGCATGAT TCAAGGGAAC TGTTCTATGA GGACCGCCAC 
TTCCACGAGG GCTGCTTCCG CTGCTGCCGA TGCCAGCGCT CCCTTGCCGA TGAGCCCTTC 
ACCTGTCAGG ACAGTGAGCT TCTGTGTAAT GAGTGTTACT GCACAGCCTT CTCGTCCCAG 
TGTTCTGCCT GCGGGGAGAC TGTCATGCCT GGGTCCCGGA AGCTGGAGTA TGGAGGCCAG 
ACGTGGCATG AACACTGCTT TCTGTGCAGT GGCTGTGAGC AGCCGCTGGG CTCCCGCTCC 
TTCGTGCCTG ACAAGGGTGC CCACTACTGC GTGCCTTGCT ATGAGAACAA ATTTGCTCCT 
CGGTGTGCCC GCTGCAGCAA GACGCTGACC CAGGGTGGAG TGACATATCG TGATCAGCCC 
TGGCACCGAG AGTGCCTGGT CTGCACTGGG TGCAAGACGC CCCTTGCAGG GCAGCAGTTC 
ACATCTCGGG ATGATGATCC TTACTGTGTG GCCTGCTTTG GAGAACTCTT TGCACCCAAG 
TGCAGCAGCT GCAAGCGCCC CATCACAGGT GGGAGCGGCG GCGGCGAGGG CGCAGGACTC 
GGTGGAGGCA AGTATGTGTC CTTCGAAGAC CGACATTGGC ACCACAGCTG CTTTTCCTGT 
GCCCGCTGCT CCACCTCCCT GGTGGGCCAA GGCTTTGTAC CAGACGGAGA TCAAGTTCTA 
TGCCAGGGCT GCAGCCAAGC AGGCCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fhl3 ( XM_006502766.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006502829.1 Mus musculus four and a half LIM domains 3 (Fhl3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu33354
Clone ID Related Accession (Same CDS sequence)	XM_006502766.4 , XM_006502766.3
Accession Version	XM_006502766.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 912bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	four and a half LIM domains protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006502766.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTTC TTGAGTTTGA GGGCTCACTG GGCACCGCCA CCATGAGCGA GGCATTTGAC 
TGTGCAAAAT GCAACGAGTC CTTGTACGGC CGCAAATACA TCCAGACAGA CAGTGGCCCC 
TACTGCGTTC CGTGCTATGA CAACACCTTC GCCAACACCT GTGCTGAGTG CCAGCAGCTC 
ATTGGGCATG ATTCAAGGGA ACTGTTCTAT GAGGACCGCC ACTTCCACGA GGGCTGCTTC 
CGCTGCTGCC GATGCCAGCG CTCCCTTGCC GATGAGCCCT TCACCTGTCA GGACAGTGAG 
CTTCTGTGTA ATGAGTGTTA CTGCACAGCC TTCTCGTCCC AGTGTTCTGC CTGCGGGGAG 
ACTGTCATGC CTGGGTCCCG GAAGCTGGAG TATGGAGGCC AGACGTGGCA TGAACACTGC 
TTTCTGTGCA GTGGCTGTGA GCAGCCGCTG GGCTCCCGCT CCTTCGTGCC TGACAAGGGT 
GCCCACTACT GCGTGCCTTG CTATGAGAAC AAATTTGCTC CTCGGTGTGC CCGCTGCAGC 
AAGACGCTGA CCCAGGGTGG AGTGACATAT CGTGATCAGC CCTGGCACCG AGAGTGCCTG 
GTCTGCACTG GGTGCAAGAC GCCCCTTGCA GGGCAGCAGT TCACATCTCG GGATGATGAT 
CCTTACTGTG TGGCCTGCTT TGGAGAACTC TTTGCACCCA AGTGCAGCAG CTGCAAGCGC 
CCCATCACAG GTGGGAGCGG CGGCGGCGAG GGCGCAGGAC TCGGTGGAGG CAAGTATGTG 
TCCTTCGAAG ACCGACATTG GCACCACAGC TGCTTTTCCT GTGCCCGCTG CTCCACCTCC 
CTGGTGGGCC AAGGCTTTGT ACCAGACGGA GATCAAGTTC TATGCCAGGG CTGCAGCCAA 
GCAGGCCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006502829.1
CDS	84..995
Translation	MGLLEFEGSLGTATMSEAFDCAKCNESLYGRKYIQTDSGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNECYCTAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQGGVTYRDQPWHRECLVCTGCKTPLAGQQFTSRDDDPYCVACFGELFAPKCSSCKRPITGGSGGGEGAGLGGGKYVSFEDRHWHHSCFSCARCSTSLVGQGFVPDGDQVLCQGCSQAGP

Target ORF information:

RefSeq Version	XM_006502766.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus four and a half LIM domains 3 (Fhl3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006502766.4

ORF Insert Sequence:

ATGGGGCTTC TTGAGTTTGA GGGCTCACTG GGCACCGCCA CCATGAGCGA GGCATTTGAC 
TGTGCAAAAT GCAACGAGTC CTTGTACGGC CGCAAATACA TCCAGACAGA CAGTGGCCCC 
TACTGCGTTC CGTGCTATGA CAACACCTTC GCCAACACCT GTGCTGAGTG CCAGCAGCTC 
ATTGGGCATG ATTCAAGGGA ACTGTTCTAT GAGGACCGCC ACTTCCACGA GGGCTGCTTC 
CGCTGCTGCC GATGCCAGCG CTCCCTTGCC GATGAGCCCT TCACCTGTCA GGACAGTGAG 
CTTCTGTGTA ATGAGTGTTA CTGCACAGCC TTCTCGTCCC AGTGTTCTGC CTGCGGGGAG 
ACTGTCATGC CTGGGTCCCG GAAGCTGGAG TATGGAGGCC AGACGTGGCA TGAACACTGC 
TTTCTGTGCA GTGGCTGTGA GCAGCCGCTG GGCTCCCGCT CCTTCGTGCC TGACAAGGGT 
GCCCACTACT GCGTGCCTTG CTATGAGAAC AAATTTGCTC CTCGGTGTGC CCGCTGCAGC 
AAGACGCTGA CCCAGGGTGG AGTGACATAT CGTGATCAGC CCTGGCACCG AGAGTGCCTG 
GTCTGCACTG GGTGCAAGAC GCCCCTTGCA GGGCAGCAGT TCACATCTCG GGATGATGAT 
CCTTACTGTG TGGCCTGCTT TGGAGAACTC TTTGCACCCA AGTGCAGCAG CTGCAAGCGC 
CCCATCACAG GTGGGAGCGG CGGCGGCGAG GGCGCAGGAC TCGGTGGAGG CAAGTATGTG 
TCCTTCGAAG ACCGACATTG GCACCACAGC TGCTTTTCCT GTGCCCGCTG CTCCACCTCC 
CTGGTGGGCC AAGGCTTTGT ACCAGACGGA GATCAAGTTC TATGCCAGGG CTGCAGCCAA 
GCAGGCCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fhl3 ( XM_006502769.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006502832.1 Mus musculus four and a half LIM domains 3 (Fhl3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu33356
Clone ID Related Accession (Same CDS sequence)	XM_006502769.3 , XM_006502769.4
Accession Version	XM_006502769.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	four and a half LIM domains protein 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006502769.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTTC TTGAGTTTGA GGGCTCACTG GGCACCGCCA CCATGAGCGA GGCATTTGAC 
TGTGCAAAAT GCAACGAGTC CTTGTACGGC CGCAAATACA TCCAGACAGA CAGTGGCCCC 
TACTGCGTTC CGTGCTATGA CAACACCTTC GCCAACACCT GTGCTGAGTG CCAGCAGCTC 
ATTGGGCATG ATTCAAGGGA ACTGTTCTAT GAGGACCGCC ACTTCCACGA GGGCTGCTTC 
CGCTGCTGCC GATGCCAGCG CTCCCTTGCC GATGAGCCCT TCACCTGTCA GGACAGTGAG 
CTTCTGTGTA ATGAGTGTTA CTGCACAGCC TTCTCGTCCC AGTGTTCTGC CTGCGGGGAG 
ACTGTCATGC CTGGGTCCCG GAAGCTGGAG TATGGAGGCC AGACGTGGCA TGAACACTGC 
TTTCTGTGCA GTGGCTGTGA GCAGCCGCTG GGCTCCCGCT CCTTCGTGCC TGACAAGGGT 
GCCCACTACT GCGTGCCTTG CTATGAGAAC AAATTTGCTC CTCGGTGTGC CCGCTGCAGC 
AAGGTTGGGG CTGGGCCTGG GAATTCAGGA CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006502832.1
CDS	87..662
Translation	MGLLEFEGSLGTATMSEAFDCAKCNESLYGRKYIQTDSGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNECYCTAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKVGAGPGNSGR

Target ORF information:

RefSeq Version	XM_006502769.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus four and a half LIM domains 3 (Fhl3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006502769.4

ORF Insert Sequence:

ATGGGGCTTC TTGAGTTTGA GGGCTCACTG GGCACCGCCA CCATGAGCGA GGCATTTGAC 
TGTGCAAAAT GCAACGAGTC CTTGTACGGC CGCAAATACA TCCAGACAGA CAGTGGCCCC 
TACTGCGTTC CGTGCTATGA CAACACCTTC GCCAACACCT GTGCTGAGTG CCAGCAGCTC 
ATTGGGCATG ATTCAAGGGA ACTGTTCTAT GAGGACCGCC ACTTCCACGA GGGCTGCTTC 
CGCTGCTGCC GATGCCAGCG CTCCCTTGCC GATGAGCCCT TCACCTGTCA GGACAGTGAG 
CTTCTGTGTA ATGAGTGTTA CTGCACAGCC TTCTCGTCCC AGTGTTCTGC CTGCGGGGAG 
ACTGTCATGC CTGGGTCCCG GAAGCTGGAG TATGGAGGCC AGACGTGGCA TGAACACTGC 
TTTCTGTGCA GTGGCTGTGA GCAGCCGCTG GGCTCCCGCT CCTTCGTGCC TGACAAGGGT 
GCCCACTACT GCGTGCCTTG CTATGAGAAC AAATTTGCTC CTCGGTGTGC CCGCTGCAGC 
AAGGTTGGGG CTGGGCCTGG GAATTCAGGA CGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

FHL3 differentially regulates the expression of MyHC isoforms through interactions with MyoD and pCREB.
Cellular signalling28(1)60-73(2016 Jan)
Zhang Y,Li W,Zhu M,Li Y,Xu Z,Zuo B

In vivo and in vitro investigations of heterozygous nebulin knock-out mice disclose a mild skeletal muscle phenotype.
Neuromuscular disorders : NMD23(4)357-69(2013 Apr)
Gineste C,De Winter JM,Kohl C,Witt CC,Giannesini B,Brohm K,Le Fur Y,Gretz N,Vilmen C,Pecchi E,Jubeau M,Cozzone PJ,Stienen GJ,Granzier H,Labeit S,Ottenheijm CA,Bendahan D,Gondin J

Screening large numbers of expression patterns of transcription factors in late stages of the mouse thymus.
Gene expression patterns : GEP11(1-2)84-92(2011 Jan-Feb)
Chung YC,Tsai YJ,Shiu TY,Sun YY,Wang PF,Chen CL

Human four-and-a-half LIM family members suppress tumor cell growth through a TGF-beta-like signaling pathway.
The Journal of clinical investigation119(2)349-61(2009 Feb)
Ding L,Wang Z,Yan J,Yang X,Liu A,Qiu W,Zhu J,Han J,Zhang H,Lin J,Cheng L,Qin X,Niu C,Yuan B,Wang X,Zhu C,Zhou Y,Li J,Song H,Huang C,Ye Q

FHL3 binds MyoD and negatively regulates myotube formation.
Journal of cell science120(Pt 8)1423-35(2007 Apr)
Cottle DL,McGrath MJ,Cowling BS,Coghill ID,Brown S,Mitchell CA

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Fhl3 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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