FHL3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	FHL3
Entrez Gene ID	2275
Full Name	four and a half LIM domains 3
Synonyms	SLIM2
General protein information	Preferred Names four and a half LIM domains 3 Names four and a half LIM domains protein 3 FHL-3 LIM-only protein FHL3 SLIM-2 skeletal muscle LIM-protein 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p34.3
Summary	The protein encoded by this gene is a member of a family of proteins containing a four-and-a-half LIM domain, which is a highly conserved double zinc finger motif. The encoded protein has been shown to interact with the cancer developmental regulators SMAD2, SMAD3, and SMAD4, the skeletal muscle myogenesis protein MyoD, and the high-affinity IgE beta chain regulator MZF-1. This protein may be involved in tumor suppression, repression of MyoD expression, and repression of IgE receptor expression. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011].
Disorder MIM:	602790

mRNA and Protein(s)

mRNA	Protein	Name
XM_024454101.1	XP_024309869.1	four and a half LIM domains protein 3 isoform X1
NM_004468.4	NP_004459.2	four and a half LIM domains protein 3 isoform 1
NM_001243878.1	NP_001230807.1	four and a half LIM domains protein 3 isoform 2
NM_004468.5	NP_004459.2	four and a half LIM domains protein 3 isoform 1
XM_024454100.1	XP_024309868.1	four and a half LIM domains protein 3 isoform X1
NM_001243878.2	NP_001230807.1	four and a half LIM domains protein 3 isoform 2
XM_024454099.1	XP_024309867.1	four and a half LIM domains protein 3 isoform X1
XM_017000675.1	XP_016856164.1	four and a half LIM domains protein 3 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FHL3	XP_003308038.1
Homo sapiens (human)	FHL3	NP_004459.2
	FHL3	XP_001112977.2
Rattus norvegicus (Norway rat)	Fhl3	NP_001101449.1
Danio rerio (zebrafish)	fhl3a	XP_695478.2
Xenopus tropicalis (tropical clawed frog)	fhl3	NP_001008165.1
Canis lupus familiaris (dog)	FHL3	XP_850071.2
Mus musculus (house mouse)	Fhl3	NP_034343.2
Gallus gallus (chicken)	FHL3	XP_004947806.1
Bos taurus (cattle)	FHL3	NP_001029395.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Yachie N, Petsalaki E, Mellor JC, Weile J, Jacob Y, Verby M, Ozturk SB, Li S, Cote AG, Mosca R, Knapp JJ, Ko M, Yu A, Gebbia M, Sahni N, Yi S, Tyagi T, Sheykhkarimli D, Roth JF, Wong C, Musa L, Snider J, Liu YC, Yu H, Braun P, Stagljar I, Hao T, Calderwood MA, Pelletier L, Aloy P, Hill DE, Vidal M, Roth FP
Molecular systems biology12(4)863(2016 Apr)

An inter-species protein-protein interaction network across vast evolutionary distance.
Zhong Q, Pevzner SJ, Hao T, Wang Y, Mosca R, Menche J, Taipale M, Ta?an M, Fan C, Yang X, Haley P, Murray RR, Mer F, Gebreab F, Tam S, MacWilliams A, Dricot A, Reichert P, Santhanam B, Ghamsari L, Calderwood MA, Rolland T, Charloteaux B, Lindquist S, Barab?si AL, Hill DE, Aloy P, Cusick ME, Xia Y, Roth FP, Vidal M
Molecular systems biology12(4)865(2016 Apr)

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

Widespread macromolecular interaction perturbations in human genetic disorders.
Sahni N, Yi S, Taipale M, Fuxman Bass JI, Coulombe-Huntington J, Yang F, Peng J, Weile J, Karras GI, Wang Y, Kov?cs IA, Kamburov A, Krykbaeva I, Lam MH, Tucker G, Khurana V, Sharma A, Liu YY, Yachie N, Zhong Q, Shen Y, Palagi A, San-Miguel A, Fan C, Balcha D, Dricot A, Jordan DM, Walsh JM, Shah AA, Yang X, Stoyanova AK, Leighton A, Calderwood MA, Jacob Y, Cusick ME, Salehi-Ashtiani K, Whitesell LJ, Sunyaev S, Berger B, Barab?si AL, Charloteaux B, Hill DE, Hao T, Roth FP, Xia Y, Walhout AJM, Lindquist S, Vidal M
Cell161(3)647-660(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the present study established a link between Ang and FHL3 proteins and identifies a new pathway for regulating astrocytoma progression.
Title: Angiogenin promotes U87MG cell proliferation by activating NF-?B signaling pathway and downregulating its binding partner FHL3.

PCBP2 could bind to FHL3 mRNA 3'UTR-A and inhibited the expression of FHL3 in T98G glioms cells
Title: Ribotrap analysis of proteins associated with FHL3 3'untranslated region in glioma cells.

These findings suggest that functional interactions between FHL3 and MT-1X may provide some clues to the mechanisms of FHL3-regulated cell proliferation.
Title: Identification and characterization of MT-1X as a novel FHL3-binding partner.

FHL3 enhances Tax1-mediated activation of the Human T-cell leukemia virus type 1 long terminal repeat (LTR) without affecting basal activity and regulates NF-kappaB activation by Tax1 in a cell-specific manner.
Title: The four and a half LIM family members are novel interactants of the human T-cell leukemia virus type 1 Tax oncoprotein.

findings suggest that the interaction between Ang and FHL3 may provide some clues to the mechanisms of Ang-regulated cell growth and apoptosis
Title: Identification and characterization of FHL3 as a novel angiogenin-binding partner.

The following FHL3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FHL3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04385	NM_004468.5 Latest version!	Homo sapiens four and a half LIM domains 3 (FHL3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu55921	NM_001243878.2 Latest version!	Homo sapiens four and a half LIM domains 3 (FHL3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu04385	XM_024454101.1 Latest version!	Homo sapiens four and a half LIM domains 3 (FHL3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu04385	XM_024454100.1 Latest version!	Homo sapiens four and a half LIM domains 3 (FHL3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu04385	XM_024454099.1 Latest version!	Homo sapiens four and a half LIM domains 3 (FHL3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu83348	XM_017000675.1 Latest version!	Homo sapiens four and a half LIM domains 3 (FHL3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OHu04385	NM_004468.4	Homo sapiens four and a half LIM domains 3 (FHL3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu55921	NM_001243878.1	Homo sapiens four and a half LIM domains 3 (FHL3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FHL3 ( NM_004468.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004459.2 Homo sapiens four and a half LIM domains 3 (FHL3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04385
Clone ID Related Accession (Same CDS sequence)	XM_024454101.1 , NM_004468.4 , NM_004468.5 , XM_024454100.1 , XM_024454099.1
Accession Version	NM_004468.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-28
Organism	Homo sapiens(human)
Product	four and a half LIM domains protein 3 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK290641.1 and BC001351.2. On Sep 1, 2011 this sequence version replaced NM_004468.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC001351.2, SRR1163655.14275.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCGAGT CATTTGACTG TGCAAAATGC AACGAGTCCC TGTATGGACG CAAGTACATC 
CAGACAGACA GCGGCCCCTA CTGTGTGCCC TGCTATGACA ATACCTTTGC CAACACCTGT 
GCTGAGTGCC AGCAGCTTAT CGGGCATGAC TCGAGGGAGC TGTTCTATGA AGACCGCCAT 
TTCCACGAGG GCTGCTTCCG CTGCTGCCGC TGCCAGCGCT CACTAGCCGA TGAACCCTTC 
ACCTGCCAGG ACAGTGAGCT GCTCTGCAAT GACTGCTACT GCAGTGCGTT TTCCTCGCAG 
TGCTCCGCTT GTGGGGAGAC TGTCATGCCT GGGTCCCGGA AGCTGGAATA TGGAGGCCAG 
ACATGGCATG AGCACTGCTT CCTGTGCAGT GGCTGTGAAC AGCCACTGGG CTCCCGTTCT 
TTTGTGCCCG ACAAGGGTGC TCACTACTGC GTGCCCTGCT ATGAGAACAA GTTTGCTCCT 
CGCTGCGCCC GCTGCAGCAA GACGCTGACA CAGGGTGGAG TGACATACCG TGATCAGCCG 
TGGCATCGAG AATGTCTGGT CTGTACCGGA TGCCAGACGC CCCTGGCAGG GCAGCAGTTC 
ACCTCCCGGG ATGAAGATCC CTACTGTGTG GCCTGTTTTG GAGAACTCTT TGCACCTAAG 
TGCAGCAGCT GCAAGCGCCC CATCGTAGGA CTCGGTGGAG GCAAGTATGT GTCCTTTGAA 
GACCGACACT GGCACCACAA CTGCTTCTCC TGCGCCCGCT GCTCTACCTC CCTGGTGGGC 
CAGGGCTTCG TACCGGATGG AGACCAAGTG CTCTGCCAGG GCTGTAGCCA GGCAGGGCCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004459.2
CDS	180..1022
Translation	MSESFDCAKCNESLYGRKYIQTDSGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNDCYCSAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQGGVTYRDQPWHRECLVCTGCQTPLAGQQFTSRDEDPYCVACFGELFAPKCSSCKRPIVGLGGGKYVSFEDRHWHHNCFSCARCSTSLVGQGFVPDGDQVLCQGCSQAGP

Target ORF information:

RefSeq Version	NM_004468.4
Organism	Homo sapiens(human)
Definition	Homo sapiens four and a half LIM domains 3 (FHL3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004468.4

ORF Insert Sequence:

ATGAGCGAGT CATTTGACTG TGCAAAATGC AACGAGTCCC TGTATGGACG CAAGTACATC 
CAGACAGACA GCGGCCCCTA CTGTGTGCCC TGCTATGACA ATACCTTTGC CAACACCTGT 
GCTGAGTGCC AGCAGCTTAT CGGGCATGAC TCGAGGGAGC TGTTCTATGA AGACCGCCAT 
TTCCACGAGG GCTGCTTCCG CTGCTGCCGC TGCCAGCGCT CACTAGCCGA TGAACCCTTC 
ACCTGCCAGG ACAGTGAGCT GCTCTGCAAT GACTGCTACT GCAGTGCGTT TTCCTCGCAG 
TGCTCCGCTT GTGGGGAGAC TGTCATGCCT GGGTCCCGGA AGCTGGAATA TGGAGGCCAG 
ACATGGCATG AGCACTGCTT CCTGTGCAGT GGCTGTGAAC AGCCACTGGG CTCCCGTTCT 
TTTGTGCCCG ACAAGGGTGC TCACTACTGC GTGCCCTGCT ATGAGAACAA GTTTGCTCCT 
CGCTGCGCCC GCTGCAGCAA GACGCTGACA CAGGGTGGAG TGACATACCG TGATCAGCCG 
TGGCATCGAG AATGTCTGGT CTGTACCGGA TGCCAGACGC CCCTGGCAGG GCAGCAGTTC 
ACCTCCCGGG ATGAAGATCC CTACTGTGTG GCCTGTTTTG GAGAACTCTT TGCACCTAAG 
TGCAGCAGCT GCAAGCGCCC CATCGTAGGA CTCGGTGGAG GCAAGTATGT GTCCTTTGAA 
GACCGACACT GGCACCACAA CTGCTTCTCC TGCGCCCGCT GCTCTACCTC CCTGGTGGGC 
CAGGGCTTCG TACCGGATGG AGACCAAGTG CTCTGCCAGG GCTGTAGCCA GGCAGGGCCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FHL3 ( NM_004468.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_004459.2 Homo sapiens four and a half LIM domains 3 (FHL3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu04385
Clone ID Related Accession (Same CDS sequence)	XM_024454101.1 , NM_004468.4 , NM_004468.5 , XM_024454100.1 , XM_024454099.1
Accession Version	NM_004468.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	four and a half LIM domains protein 3 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL603790.41. On Nov 23, 2018 this sequence version replaced NM_004468.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC001351.2, SRR1163655.14275.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000373016.4/ ENSP00000362107.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGCGAGT CATTTGACTG TGCAAAATGC AACGAGTCCC TGTATGGACG CAAGTACATC 
CAGACAGACA GCGGCCCCTA CTGTGTGCCC TGCTATGACA ATACCTTTGC CAACACCTGT 
GCTGAGTGCC AGCAGCTTAT CGGGCATGAC TCGAGGGAGC TGTTCTATGA AGACCGCCAT 
TTCCACGAGG GCTGCTTCCG CTGCTGCCGC TGCCAGCGCT CACTAGCCGA TGAACCCTTC 
ACCTGCCAGG ACAGTGAGCT GCTCTGCAAT GACTGCTACT GCAGTGCGTT TTCCTCGCAG 
TGCTCCGCTT GTGGGGAGAC TGTCATGCCT GGGTCCCGGA AGCTGGAATA TGGAGGCCAG 
ACATGGCATG AGCACTGCTT CCTGTGCAGT GGCTGTGAAC AGCCACTGGG CTCCCGTTCT 
TTTGTGCCCG ACAAGGGTGC TCACTACTGC GTGCCCTGCT ATGAGAACAA GTTTGCTCCT 
CGCTGCGCCC GCTGCAGCAA GACGCTGACA CAGGGTGGAG TGACATACCG TGATCAGCCG 
TGGCATCGAG AATGTCTGGT CTGTACCGGA TGCCAGACGC CCCTGGCAGG GCAGCAGTTC 
ACCTCCCGGG ATGAAGATCC CTACTGTGTG GCCTGTTTTG GAGAACTCTT TGCACCTAAG 
TGCAGCAGCT GCAAGCGCCC CATCGTAGGA CTCGGTGGAG GCAAGTATGT GTCCTTTGAA 
GACCGACACT GGCACCACAA CTGCTTCTCC TGCGCCCGCT GCTCTACCTC CCTGGTGGGC 
CAGGGCTTCG TACCGGATGG AGACCAAGTG CTCTGCCAGG GCTGTAGCCA GGCAGGGCCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004459.2
CDS	179..1021
Translation	MSESFDCAKCNESLYGRKYIQTDSGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNDCYCSAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQGGVTYRDQPWHRECLVCTGCQTPLAGQQFTSRDEDPYCVACFGELFAPKCSSCKRPIVGLGGGKYVSFEDRHWHHNCFSCARCSTSLVGQGFVPDGDQVLCQGCSQAGP

Target ORF information:

RefSeq Version	NM_004468.5
Organism	Homo sapiens(human)
Definition	Homo sapiens four and a half LIM domains 3 (FHL3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004468.5

ORF Insert Sequence:

ATGAGCGAGT CATTTGACTG TGCAAAATGC AACGAGTCCC TGTATGGACG CAAGTACATC 
CAGACAGACA GCGGCCCCTA CTGTGTGCCC TGCTATGACA ATACCTTTGC CAACACCTGT 
GCTGAGTGCC AGCAGCTTAT CGGGCATGAC TCGAGGGAGC TGTTCTATGA AGACCGCCAT 
TTCCACGAGG GCTGCTTCCG CTGCTGCCGC TGCCAGCGCT CACTAGCCGA TGAACCCTTC 
ACCTGCCAGG ACAGTGAGCT GCTCTGCAAT GACTGCTACT GCAGTGCGTT TTCCTCGCAG 
TGCTCCGCTT GTGGGGAGAC TGTCATGCCT GGGTCCCGGA AGCTGGAATA TGGAGGCCAG 
ACATGGCATG AGCACTGCTT CCTGTGCAGT GGCTGTGAAC AGCCACTGGG CTCCCGTTCT 
TTTGTGCCCG ACAAGGGTGC TCACTACTGC GTGCCCTGCT ATGAGAACAA GTTTGCTCCT 
CGCTGCGCCC GCTGCAGCAA GACGCTGACA CAGGGTGGAG TGACATACCG TGATCAGCCG 
TGGCATCGAG AATGTCTGGT CTGTACCGGA TGCCAGACGC CCCTGGCAGG GCAGCAGTTC 
ACCTCCCGGG ATGAAGATCC CTACTGTGTG GCCTGTTTTG GAGAACTCTT TGCACCTAAG 
TGCAGCAGCT GCAAGCGCCC CATCGTAGGA CTCGGTGGAG GCAAGTATGT GTCCTTTGAA 
GACCGACACT GGCACCACAA CTGCTTCTCC TGCGCCCGCT GCTCTACCTC CCTGGTGGGC 
CAGGGCTTCG TACCGGATGG AGACCAAGTG CTCTGCCAGG GCTGTAGCCA GGCAGGGCCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FHL3 ( NM_001243878.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001230807.1 Homo sapiens four and a half LIM domains 3 (FHL3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu55921
Clone ID Related Accession (Same CDS sequence)	NM_001243878.1 , NM_001243878.2
Accession Version	NM_001243878.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-14
Organism	Homo sapiens(human)
Product	four and a half LIM domains protein 3 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK290641.1, BC014501.1 and BC001351.2. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC014501.1, ERR279845.7958.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTGGGT CCCGGAAGCT GGAATATGGA GGCCAGACAT GGCATGAGCA CTGCTTCCTG 
TGCAGTGGCT GTGAACAGCC ACTGGGCTCC CGTTCTTTTG TGCCCGACAA GGGTGCTCAC 
TACTGCGTGC CCTGCTATGA GAACAAGTTT GCTCCTCGCT GCGCCCGCTG CAGCAAGACG 
CTGACACAGG GTGGAGTGAC ATACCGTGAT CAGCCGTGGC ATCGAGAATG TCTGGTCTGT 
ACCGGATGCC AGACGCCCCT GGCAGGGCAG CAGTTCACCT CCCGGGATGA AGATCCCTAC 
TGTGTGGCCT GTTTTGGAGA ACTCTTTGCA CCTAAGTGCA GCAGCTGCAA GCGCCCCATC 
GTAGGACTCG GTGGAGGCAA GTATGTGTCC TTTGAAGACC GACACTGGCA CCACAACTGC 
TTCTCCTGCG CCCGCTGCTC TACCTCCCTG GTGGGCCAGG GCTTCGTACC GGATGGAGAC 
CAAGTGCTCT GCCAGGGCTG TAGCCAGGCA GGGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001230807.1
CDS	328..846
Translation	MPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQGGVTYRDQPWHRECLVCTGCQTPLAGQQFTSRDEDPYCVACFGELFAPKCSSCKRPIVGLGGGKYVSFEDRHWHHNCFSCARCSTSLVGQGFVPDGDQVLCQGCSQAGP

Target ORF information:

RefSeq Version	NM_001243878.1
Organism	Homo sapiens(human)
Definition	Homo sapiens four and a half LIM domains 3 (FHL3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001243878.1

ORF Insert Sequence:

ATGCCTGGGT CCCGGAAGCT GGAATATGGA GGCCAGACAT GGCATGAGCA CTGCTTCCTG 
TGCAGTGGCT GTGAACAGCC ACTGGGCTCC CGTTCTTTTG TGCCCGACAA GGGTGCTCAC 
TACTGCGTGC CCTGCTATGA GAACAAGTTT GCTCCTCGCT GCGCCCGCTG CAGCAAGACG 
CTGACACAGG GTGGAGTGAC ATACCGTGAT CAGCCGTGGC ATCGAGAATG TCTGGTCTGT 
ACCGGATGCC AGACGCCCCT GGCAGGGCAG CAGTTCACCT CCCGGGATGA AGATCCCTAC 
TGTGTGGCCT GTTTTGGAGA ACTCTTTGCA CCTAAGTGCA GCAGCTGCAA GCGCCCCATC 
GTAGGACTCG GTGGAGGCAA GTATGTGTCC TTTGAAGACC GACACTGGCA CCACAACTGC 
TTCTCCTGCG CCCGCTGCTC TACCTCCCTG GTGGGCCAGG GCTTCGTACC GGATGGAGAC 
CAAGTGCTCT GCCAGGGCTG TAGCCAGGCA GGGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FHL3 ( NM_001243878.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001230807.1 Homo sapiens four and a half LIM domains 3 (FHL3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu55921
Clone ID Related Accession (Same CDS sequence)	NM_001243878.1 , NM_001243878.2
Accession Version	NM_001243878.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 519bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	four and a half LIM domains protein 3 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL603790.41. On May 31, 2019 this sequence version replaced NM_001243878.1. Transcript Variant: This variant (2) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC014501.1, ERR279829.3105.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTGGGT CCCGGAAGCT GGAATATGGA GGCCAGACAT GGCATGAGCA CTGCTTCCTG 
TGCAGTGGCT GTGAACAGCC ACTGGGCTCC CGTTCTTTTG TGCCCGACAA GGGTGCTCAC 
TACTGCGTGC CCTGCTATGA GAACAAGTTT GCTCCTCGCT GCGCCCGCTG CAGCAAGACG 
CTGACACAGG GTGGAGTGAC ATACCGTGAT CAGCCGTGGC ATCGAGAATG TCTGGTCTGT 
ACCGGATGCC AGACGCCCCT GGCAGGGCAG CAGTTCACCT CCCGGGATGA AGATCCCTAC 
TGTGTGGCCT GTTTTGGAGA ACTCTTTGCA CCTAAGTGCA GCAGCTGCAA GCGCCCCATC 
GTAGGACTCG GTGGAGGCAA GTATGTGTCC TTTGAAGACC GACACTGGCA CCACAACTGC 
TTCTCCTGCG CCCGCTGCTC TACCTCCCTG GTGGGCCAGG GCTTCGTACC GGATGGAGAC 
CAAGTGCTCT GCCAGGGCTG TAGCCAGGCA GGGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001230807.1
CDS	327..845
Translation	MPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQGGVTYRDQPWHRECLVCTGCQTPLAGQQFTSRDEDPYCVACFGELFAPKCSSCKRPIVGLGGGKYVSFEDRHWHHNCFSCARCSTSLVGQGFVPDGDQVLCQGCSQAGP

Target ORF information:

RefSeq Version	NM_001243878.2
Organism	Homo sapiens(human)
Definition	Homo sapiens four and a half LIM domains 3 (FHL3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001243878.2

ORF Insert Sequence:

ATGCCTGGGT CCCGGAAGCT GGAATATGGA GGCCAGACAT GGCATGAGCA CTGCTTCCTG 
TGCAGTGGCT GTGAACAGCC ACTGGGCTCC CGTTCTTTTG TGCCCGACAA GGGTGCTCAC 
TACTGCGTGC CCTGCTATGA GAACAAGTTT GCTCCTCGCT GCGCCCGCTG CAGCAAGACG 
CTGACACAGG GTGGAGTGAC ATACCGTGAT CAGCCGTGGC ATCGAGAATG TCTGGTCTGT 
ACCGGATGCC AGACGCCCCT GGCAGGGCAG CAGTTCACCT CCCGGGATGA AGATCCCTAC 
TGTGTGGCCT GTTTTGGAGA ACTCTTTGCA CCTAAGTGCA GCAGCTGCAA GCGCCCCATC 
GTAGGACTCG GTGGAGGCAA GTATGTGTCC TTTGAAGACC GACACTGGCA CCACAACTGC 
TTCTCCTGCG CCCGCTGCTC TACCTCCCTG GTGGGCCAGG GCTTCGTACC GGATGGAGAC 
CAAGTGCTCT GCCAGGGCTG TAGCCAGGCA GGGCCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FHL3 ( XM_024454101.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309869.1 Homo sapiens four and a half LIM domains 3 (FHL3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu04385
Clone ID Related Accession (Same CDS sequence)	XM_024454101.1 , NM_004468.4 , NM_004468.5 , XM_024454100.1 , XM_024454099.1
Accession Version	XM_024454101.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	four and a half LIM domains protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGT CATTTGACTG TGCAAAATGC AACGAGTCCC TGTATGGACG CAAGTACATC 
CAGACAGACA GCGGCCCCTA CTGTGTGCCC TGCTATGACA ATACCTTTGC CAACACCTGT 
GCTGAGTGCC AGCAGCTTAT CGGGCATGAC TCGAGGGAGC TGTTCTATGA AGACCGCCAT 
TTCCACGAGG GCTGCTTCCG CTGCTGCCGC TGCCAGCGCT CACTAGCCGA TGAACCCTTC 
ACCTGCCAGG ACAGTGAGCT GCTCTGCAAT GACTGCTACT GCAGTGCGTT TTCCTCGCAG 
TGCTCCGCTT GTGGGGAGAC TGTCATGCCT GGGTCCCGGA AGCTGGAATA TGGAGGCCAG 
ACATGGCATG AGCACTGCTT CCTGTGCAGT GGCTGTGAAC AGCCACTGGG CTCCCGTTCT 
TTTGTGCCCG ACAAGGGTGC TCACTACTGC GTGCCCTGCT ATGAGAACAA GTTTGCTCCT 
CGCTGCGCCC GCTGCAGCAA GACGCTGACA CAGGGTGGAG TGACATACCG TGATCAGCCG 
TGGCATCGAG AATGTCTGGT CTGTACCGGA TGCCAGACGC CCCTGGCAGG GCAGCAGTTC 
ACCTCCCGGG ATGAAGATCC CTACTGTGTG GCCTGTTTTG GAGAACTCTT TGCACCTAAG 
TGCAGCAGCT GCAAGCGCCC CATCGTAGGA CTCGGTGGAG GCAAGTATGT GTCCTTTGAA 
GACCGACACT GGCACCACAA CTGCTTCTCC TGCGCCCGCT GCTCTACCTC CCTGGTGGGC 
CAGGGCTTCG TACCGGATGG AGACCAAGTG CTCTGCCAGG GCTGTAGCCA GGCAGGGCCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309869.1
CDS	40..882
Translation	MSESFDCAKCNESLYGRKYIQTDSGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNDCYCSAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQGGVTYRDQPWHRECLVCTGCQTPLAGQQFTSRDEDPYCVACFGELFAPKCSSCKRPIVGLGGGKYVSFEDRHWHHNCFSCARCSTSLVGQGFVPDGDQVLCQGCSQAGP

Target ORF information:

RefSeq Version	XM_024454101.1
Organism	Homo sapiens(human)
Definition	Homo sapiens four and a half LIM domains 3 (FHL3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024454101.1

ORF Insert Sequence:

ATGAGCGAGT CATTTGACTG TGCAAAATGC AACGAGTCCC TGTATGGACG CAAGTACATC 
CAGACAGACA GCGGCCCCTA CTGTGTGCCC TGCTATGACA ATACCTTTGC CAACACCTGT 
GCTGAGTGCC AGCAGCTTAT CGGGCATGAC TCGAGGGAGC TGTTCTATGA AGACCGCCAT 
TTCCACGAGG GCTGCTTCCG CTGCTGCCGC TGCCAGCGCT CACTAGCCGA TGAACCCTTC 
ACCTGCCAGG ACAGTGAGCT GCTCTGCAAT GACTGCTACT GCAGTGCGTT TTCCTCGCAG 
TGCTCCGCTT GTGGGGAGAC TGTCATGCCT GGGTCCCGGA AGCTGGAATA TGGAGGCCAG 
ACATGGCATG AGCACTGCTT CCTGTGCAGT GGCTGTGAAC AGCCACTGGG CTCCCGTTCT 
TTTGTGCCCG ACAAGGGTGC TCACTACTGC GTGCCCTGCT ATGAGAACAA GTTTGCTCCT 
CGCTGCGCCC GCTGCAGCAA GACGCTGACA CAGGGTGGAG TGACATACCG TGATCAGCCG 
TGGCATCGAG AATGTCTGGT CTGTACCGGA TGCCAGACGC CCCTGGCAGG GCAGCAGTTC 
ACCTCCCGGG ATGAAGATCC CTACTGTGTG GCCTGTTTTG GAGAACTCTT TGCACCTAAG 
TGCAGCAGCT GCAAGCGCCC CATCGTAGGA CTCGGTGGAG GCAAGTATGT GTCCTTTGAA 
GACCGACACT GGCACCACAA CTGCTTCTCC TGCGCCCGCT GCTCTACCTC CCTGGTGGGC 
CAGGGCTTCG TACCGGATGG AGACCAAGTG CTCTGCCAGG GCTGTAGCCA GGCAGGGCCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FHL3 ( XM_024454100.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309868.1 Homo sapiens four and a half LIM domains 3 (FHL3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu04385
Clone ID Related Accession (Same CDS sequence)	XM_024454101.1 , NM_004468.4 , NM_004468.5 , XM_024454100.1 , XM_024454099.1
Accession Version	XM_024454100.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	four and a half LIM domains protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGT CATTTGACTG TGCAAAATGC AACGAGTCCC TGTATGGACG CAAGTACATC 
CAGACAGACA GCGGCCCCTA CTGTGTGCCC TGCTATGACA ATACCTTTGC CAACACCTGT 
GCTGAGTGCC AGCAGCTTAT CGGGCATGAC TCGAGGGAGC TGTTCTATGA AGACCGCCAT 
TTCCACGAGG GCTGCTTCCG CTGCTGCCGC TGCCAGCGCT CACTAGCCGA TGAACCCTTC 
ACCTGCCAGG ACAGTGAGCT GCTCTGCAAT GACTGCTACT GCAGTGCGTT TTCCTCGCAG 
TGCTCCGCTT GTGGGGAGAC TGTCATGCCT GGGTCCCGGA AGCTGGAATA TGGAGGCCAG 
ACATGGCATG AGCACTGCTT CCTGTGCAGT GGCTGTGAAC AGCCACTGGG CTCCCGTTCT 
TTTGTGCCCG ACAAGGGTGC TCACTACTGC GTGCCCTGCT ATGAGAACAA GTTTGCTCCT 
CGCTGCGCCC GCTGCAGCAA GACGCTGACA CAGGGTGGAG TGACATACCG TGATCAGCCG 
TGGCATCGAG AATGTCTGGT CTGTACCGGA TGCCAGACGC CCCTGGCAGG GCAGCAGTTC 
ACCTCCCGGG ATGAAGATCC CTACTGTGTG GCCTGTTTTG GAGAACTCTT TGCACCTAAG 
TGCAGCAGCT GCAAGCGCCC CATCGTAGGA CTCGGTGGAG GCAAGTATGT GTCCTTTGAA 
GACCGACACT GGCACCACAA CTGCTTCTCC TGCGCCCGCT GCTCTACCTC CCTGGTGGGC 
CAGGGCTTCG TACCGGATGG AGACCAAGTG CTCTGCCAGG GCTGTAGCCA GGCAGGGCCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309868.1
CDS	44..886
Translation	MSESFDCAKCNESLYGRKYIQTDSGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNDCYCSAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQGGVTYRDQPWHRECLVCTGCQTPLAGQQFTSRDEDPYCVACFGELFAPKCSSCKRPIVGLGGGKYVSFEDRHWHHNCFSCARCSTSLVGQGFVPDGDQVLCQGCSQAGP

Target ORF information:

RefSeq Version	XM_024454100.1
Organism	Homo sapiens(human)
Definition	Homo sapiens four and a half LIM domains 3 (FHL3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024454100.1

ORF Insert Sequence:

ATGAGCGAGT CATTTGACTG TGCAAAATGC AACGAGTCCC TGTATGGACG CAAGTACATC 
CAGACAGACA GCGGCCCCTA CTGTGTGCCC TGCTATGACA ATACCTTTGC CAACACCTGT 
GCTGAGTGCC AGCAGCTTAT CGGGCATGAC TCGAGGGAGC TGTTCTATGA AGACCGCCAT 
TTCCACGAGG GCTGCTTCCG CTGCTGCCGC TGCCAGCGCT CACTAGCCGA TGAACCCTTC 
ACCTGCCAGG ACAGTGAGCT GCTCTGCAAT GACTGCTACT GCAGTGCGTT TTCCTCGCAG 
TGCTCCGCTT GTGGGGAGAC TGTCATGCCT GGGTCCCGGA AGCTGGAATA TGGAGGCCAG 
ACATGGCATG AGCACTGCTT CCTGTGCAGT GGCTGTGAAC AGCCACTGGG CTCCCGTTCT 
TTTGTGCCCG ACAAGGGTGC TCACTACTGC GTGCCCTGCT ATGAGAACAA GTTTGCTCCT 
CGCTGCGCCC GCTGCAGCAA GACGCTGACA CAGGGTGGAG TGACATACCG TGATCAGCCG 
TGGCATCGAG AATGTCTGGT CTGTACCGGA TGCCAGACGC CCCTGGCAGG GCAGCAGTTC 
ACCTCCCGGG ATGAAGATCC CTACTGTGTG GCCTGTTTTG GAGAACTCTT TGCACCTAAG 
TGCAGCAGCT GCAAGCGCCC CATCGTAGGA CTCGGTGGAG GCAAGTATGT GTCCTTTGAA 
GACCGACACT GGCACCACAA CTGCTTCTCC TGCGCCCGCT GCTCTACCTC CCTGGTGGGC 
CAGGGCTTCG TACCGGATGG AGACCAAGTG CTCTGCCAGG GCTGTAGCCA GGCAGGGCCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FHL3 ( XM_024454099.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309867.1 Homo sapiens four and a half LIM domains 3 (FHL3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu04385
Clone ID Related Accession (Same CDS sequence)	XM_024454101.1 , NM_004468.4 , NM_004468.5 , XM_024454100.1 , XM_024454099.1
Accession Version	XM_024454099.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	four and a half LIM domains protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGT CATTTGACTG TGCAAAATGC AACGAGTCCC TGTATGGACG CAAGTACATC 
CAGACAGACA GCGGCCCCTA CTGTGTGCCC TGCTATGACA ATACCTTTGC CAACACCTGT 
GCTGAGTGCC AGCAGCTTAT CGGGCATGAC TCGAGGGAGC TGTTCTATGA AGACCGCCAT 
TTCCACGAGG GCTGCTTCCG CTGCTGCCGC TGCCAGCGCT CACTAGCCGA TGAACCCTTC 
ACCTGCCAGG ACAGTGAGCT GCTCTGCAAT GACTGCTACT GCAGTGCGTT TTCCTCGCAG 
TGCTCCGCTT GTGGGGAGAC TGTCATGCCT GGGTCCCGGA AGCTGGAATA TGGAGGCCAG 
ACATGGCATG AGCACTGCTT CCTGTGCAGT GGCTGTGAAC AGCCACTGGG CTCCCGTTCT 
TTTGTGCCCG ACAAGGGTGC TCACTACTGC GTGCCCTGCT ATGAGAACAA GTTTGCTCCT 
CGCTGCGCCC GCTGCAGCAA GACGCTGACA CAGGGTGGAG TGACATACCG TGATCAGCCG 
TGGCATCGAG AATGTCTGGT CTGTACCGGA TGCCAGACGC CCCTGGCAGG GCAGCAGTTC 
ACCTCCCGGG ATGAAGATCC CTACTGTGTG GCCTGTTTTG GAGAACTCTT TGCACCTAAG 
TGCAGCAGCT GCAAGCGCCC CATCGTAGGA CTCGGTGGAG GCAAGTATGT GTCCTTTGAA 
GACCGACACT GGCACCACAA CTGCTTCTCC TGCGCCCGCT GCTCTACCTC CCTGGTGGGC 
CAGGGCTTCG TACCGGATGG AGACCAAGTG CTCTGCCAGG GCTGTAGCCA GGCAGGGCCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309867.1
CDS	98..940
Translation	MSESFDCAKCNESLYGRKYIQTDSGPYCVPCYDNTFANTCAECQQLIGHDSRELFYEDRHFHEGCFRCCRCQRSLADEPFTCQDSELLCNDCYCSAFSSQCSACGETVMPGSRKLEYGGQTWHEHCFLCSGCEQPLGSRSFVPDKGAHYCVPCYENKFAPRCARCSKTLTQGGVTYRDQPWHRECLVCTGCQTPLAGQQFTSRDEDPYCVACFGELFAPKCSSCKRPIVGLGGGKYVSFEDRHWHHNCFSCARCSTSLVGQGFVPDGDQVLCQGCSQAGP

Target ORF information:

RefSeq Version	XM_024454099.1
Organism	Homo sapiens(human)
Definition	Homo sapiens four and a half LIM domains 3 (FHL3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024454099.1

ORF Insert Sequence:

ATGAGCGAGT CATTTGACTG TGCAAAATGC AACGAGTCCC TGTATGGACG CAAGTACATC 
CAGACAGACA GCGGCCCCTA CTGTGTGCCC TGCTATGACA ATACCTTTGC CAACACCTGT 
GCTGAGTGCC AGCAGCTTAT CGGGCATGAC TCGAGGGAGC TGTTCTATGA AGACCGCCAT 
TTCCACGAGG GCTGCTTCCG CTGCTGCCGC TGCCAGCGCT CACTAGCCGA TGAACCCTTC 
ACCTGCCAGG ACAGTGAGCT GCTCTGCAAT GACTGCTACT GCAGTGCGTT TTCCTCGCAG 
TGCTCCGCTT GTGGGGAGAC TGTCATGCCT GGGTCCCGGA AGCTGGAATA TGGAGGCCAG 
ACATGGCATG AGCACTGCTT CCTGTGCAGT GGCTGTGAAC AGCCACTGGG CTCCCGTTCT 
TTTGTGCCCG ACAAGGGTGC TCACTACTGC GTGCCCTGCT ATGAGAACAA GTTTGCTCCT 
CGCTGCGCCC GCTGCAGCAA GACGCTGACA CAGGGTGGAG TGACATACCG TGATCAGCCG 
TGGCATCGAG AATGTCTGGT CTGTACCGGA TGCCAGACGC CCCTGGCAGG GCAGCAGTTC 
ACCTCCCGGG ATGAAGATCC CTACTGTGTG GCCTGTTTTG GAGAACTCTT TGCACCTAAG 
TGCAGCAGCT GCAAGCGCCC CATCGTAGGA CTCGGTGGAG GCAAGTATGT GTCCTTTGAA 
GACCGACACT GGCACCACAA CTGCTTCTCC TGCGCCCGCT GCTCTACCTC CCTGGTGGGC 
CAGGGCTTCG TACCGGATGG AGACCAAGTG CTCTGCCAGG GCTGTAGCCA GGCAGGGCCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FHL3 ( XM_017000675.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856164.1 Homo sapiens four and a half LIM domains 3 (FHL3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu83348
Clone ID Related Accession (Same CDS sequence)	XM_017000675.1
Accession Version	XM_017000675.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	four and a half LIM domains protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACCCTT CACCTGCCAG GACAGTGAGC TGCTCTGCAA TGACTGCTAC TGCAGTGCGT 
TTTCCTCGCA GTGCTCCGCT TGTGGGGAGA CTGTCATGCC TGACGCTGAC ACAGGGTGGA 
GTGACATACC GTGATCAGCC GTGGCATCGA GAATGTCTGG TCTGTACCGG ATGCCAGACG 
CCCCTGGCAG GGCAGCAGTT CACCTCCCGG GATGAAGATC CCTACTGTGT GGCCTGTTTT 
GGAGAACTCT TTGCACCTAA GTGCAGCAGC TGCAAGCGCC CCATCGTAGG ACTCGGTGGA 
GGCAAGTATG TGTCCTTTGA AGACCGACAC TGGCACCACA ACTGCTTCTC CTGCGCCCGC 
TGCTCTACCT CCCTGGTGGG CCAGGGCTTC GTACCGGATG GAGACCAAGT GCTCTGCCAG 
GGCTGTAGCC AGGCAGGGCC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856164.1
CDS	3..446
Translation	MNPSPARTVSCSAMTATAVRFPRSAPLVGRLSCLTLTQGGVTYRDQPWHRECLVCTGCQTPLAGQQFTSRDEDPYCVACFGELFAPKCSSCKRPIVGLGGGKYVSFEDRHWHHNCFSCARCSTSLVGQGFVPDGDQVLCQGCSQAGP

Target ORF information:

RefSeq Version	XM_017000675.1
Organism	Homo sapiens(human)
Definition	Homo sapiens four and a half LIM domains 3 (FHL3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000675.1

ORF Insert Sequence:

ATGAACCCTT CACCTGCCAG GACAGTGAGC TGCTCTGCAA TGACTGCTAC TGCAGTGCGT 
TTTCCTCGCA GTGCTCCGCT TGTGGGGAGA CTGTCATGCC TGACGCTGAC ACAGGGTGGA 
GTGACATACC GTGATCAGCC GTGGCATCGA GAATGTCTGG TCTGTACCGG ATGCCAGACG 
CCCCTGGCAG GGCAGCAGTT CACCTCCCGG GATGAAGATC CCTACTGTGT GGCCTGTTTT 
GGAGAACTCT TTGCACCTAA GTGCAGCAGC TGCAAGCGCC CCATCGTAGG ACTCGGTGGA 
GGCAAGTATG TGTCCTTTGA AGACCGACAC TGGCACCACA ACTGCTTCTC CTGCGCCCGC 
TGCTCTACCT CCCTGGTGGG CCAGGGCTTC GTACCGGATG GAGACCAAGT GCTCTGCCAG 
GGCTGTAGCC AGGCAGGGCC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Pooled-matrix protein interaction screens using Barcode Fusion Genetics.
Molecular systems biology12(4)863(2016 Apr)
Yachie N,Petsalaki E,Mellor JC,Weile J,Jacob Y,Verby M,Ozturk SB,Li S,Cote AG,Mosca R,Knapp JJ,Ko M,Yu A,Gebbia M,Sahni N,Yi S,Tyagi T,Sheykhkarimli D,Roth JF,Wong C,Musa L,Snider J,Liu YC,Yu H,Braun P,Stagljar I,Hao T,Calderwood MA,Pelletier L,Aloy P,Hill DE,Vidal M,Roth FP

An inter-species protein-protein interaction network across vast evolutionary distance.
Molecular systems biology12(4)865(2016 Apr)
Zhong Q,Pevzner SJ,Hao T,Wang Y,Mosca R,Menche J,Taipale M,Ta?an M,Fan C,Yang X,Haley P,Murray RR,Mer F,Gebreab F,Tam S,MacWilliams A,Dricot A,Reichert P,Santhanam B,Ghamsari L,Calderwood MA,Rolland T,Charloteaux B,Lindquist S,Barab?si AL,Hill DE,Aloy P,Cusick ME,Xia Y,Roth FP,Vidal M

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M

Widespread macromolecular interaction perturbations in human genetic disorders.
Cell161(3)647-660(2015 Apr)
Sahni N,Yi S,Taipale M,Fuxman Bass JI,Coulombe-Huntington J,Yang F,Peng J,Weile J,Karras GI,Wang Y,Kov?cs IA,Kamburov A,Krykbaeva I,Lam MH,Tucker G,Khurana V,Sharma A,Liu YY,Yachie N,Zhong Q,Shen Y,Palagi A,San-Miguel A,Fan C,Balcha D,Dricot A,Jordan DM,Walsh JM,Shah AA,Yang X,Stoyanova AK,Leighton A,Calderwood MA,Jacob Y,Cusick ME,Salehi-Ashtiani K,Whitesell LJ,Sunyaev S,Berger B,Barab?si AL,Charloteaux B,Hill DE,Hao T,Roth FP,Xia Y,Walhout AJM,Lindquist S,Vidal M

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FHL3 cDNA ORF clone, Homo sapiens(human)

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Clones

gRNAs

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ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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