Fap cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fap
Entrez Gene ID	14089
Full Name	fibroblast activation protein
Synonyms	SIMP
General protein information	Preferred Names fibroblast activation protein Names prolyl endopeptidase FAP FAPalpha dipeptidyl peptidase FAP fibroblast activation protein alpha gelatine degradation protease FAP integral membrane serine protease post-proline cleaving enzyme seprase serine integral membrane protease surface-expressed protease
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2 C1.3\|2 35.85 cM
Summary	This gene belongs to the serine protease family. The encoded protein is an inducible cell-surface bound glycoprotein specifically expressed in tumor-associated fibroblasts and pericytes of epithelial tumors and has protease and gelatinase activity. The protein plays a role in remodeling of the extracellular matrix (ECM) and may affect tumorigenesis and tissue repair. Alternately spliced transcript variants of this gene are described in the literature (PMID 9139873), but the full-length sequence of these variants is not available. [provided by RefSeq, Apr 2013].

mRNA and Protein(s)

mRNA	Protein	Name
XM_011239289.1	XP_011237591.1	prolyl endopeptidase FAP isoform X3
XM_006498746.4	XP_006498809.1	prolyl endopeptidase FAP isoform X2
XM_017315512.1	XP_017171001.1	prolyl endopeptidase FAP isoform X3
XM_006498746.3	XP_006498809.1	prolyl endopeptidase FAP isoform X2
NM_007986.3	NP_032012.1	prolyl endopeptidase FAP
XM_017315507.1	XP_017170996.1	prolyl endopeptidase FAP isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Fap	NP_032012.1
Gallus gallus (chicken)	FAP	NP_001180568.1
Pan troglodytes (chimpanzee)	FAP	XP_515860.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011584	XP_309924.4
Xenopus tropicalis (tropical clawed frog)	fap	NP_001107145.1
Drosophila melanogaster (fruit fly)	ome	NP_001097606.1
	FAP	XP_001096858.1
Bos taurus (cattle)	FAP	NP_001091470.1
Rattus norvegicus (Norway rat)	Fap	NP_620205.1
Magnaporthe oryzae (rice blast fungus)	MGG_05989	XP_003711833.1
Homo sapiens (human)	FAP	NP_004451.2
Canis lupus familiaris (dog)	FAP	XP_005640309.1

Gene Ontology
Bibliography

Related articles in PubMed

FAPalpha, a surface peptidase expressed during wound healing, is a tumor suppressor.
Ramirez-Montagut T, Blachere NE, Sviderskaya EV, Bennett DC, Rettig WJ, Garin-Chesa P, Houghton AN
Oncogene23(32)5435-46(2004 Jul)

Gene-expression profile of collagen-induced arthritis.
Ibrahim SM, Koczan D, Thiesen HJ
Journal of autoimmunity18(2)159-67(2002 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Taken together, our study suggested that high FAP expression in CAFs is one reason leading to immune checkpoint blockades resistance in CRC patients and FAP is an optional target for reversing immune checkpoint blockades resistance.
Title: FAP positive fibroblasts induce immune checkpoint blockade resistance in colorectal cancer via promoting immunosuppression.

FAP-vaccinated mice also treated with Cyclophosphamide chemotherapy showed a marked suppression of tumor growth (inhibition ratio =80%) and a prolongation of survival time.
Title: Cyclophosphamide enhances anti-tumor effects of a fibroblast activation protein ?-based DNA vaccine in tumor-bearing mice with murine breast carcinoma.

There was no evidence of compensatory upregulation of other DPP4 family members in influenza-infected FAP-deficient mice. FAP appears to be dispensable in anti-influenza adaptive immunity.
Title: Fibroblast activation protein is dispensable in the anti-influenza immune response in mice.

this study shows that FAPalpha-targeted modified vaccinia ankara boosting in combination with cyclophosphamide is an effective approach to improving specific anti-tumor immune responses through overcoming immunosuppression
Title: Improvement of anti-tumor immunity of fibroblast activation protein ? based vaccines by combination with cyclophosphamide in a murine model of breast cancer.

FAP-STAT3-CCL2 signaling in Cancer-associated fibroblasts (CAF) was sufficient to program an inflammatory component of the tumor microenvironment, which may have particular significance in desmoplasia-associated cancers.
Title: FAP Promotes Immunosuppression by Cancer-Associated Fibroblasts in the Tumor Microenvironment via STAT3-CCL2 Signaling.

The following Fap gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fap cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu67719	XM_017315512.1 Latest version!	Mus musculus fibroblast activation protein (Fap), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu67719	XM_011239289.1 Latest version!	Mus musculus fibroblast activation protein (Fap), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OMu01178	NM_007986.3 Latest version!	Mus musculus fibroblast activation protein (Fap), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu01178	XM_006498746.4 Latest version!	Mus musculus fibroblast activation protein (Fap), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu80065	XM_017315507.1 Latest version!	Mus musculus fibroblast activation protein (Fap), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu01178	XM_006498746.3	Mus musculus fibroblast activation protein (Fap), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fap ( XM_011239289.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011237591.1 Mus musculus fibroblast activation protein (Fap), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu67719
Clone ID Related Accession (Same CDS sequence)	XM_011239289.1 , XM_017315512.1
Accession Version	XM_011239289.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	prolyl endopeptidase FAP isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAAA ACTGTCTTTG GAGTTACCAC CCTGGCTGCG CTTGCTTTAG TGGTGATATG 
CATTGTCTTA CGTCCCTCAA GAGGGAATTT GTAAGAGGAT ACGAGCTCCC TCGTCCAATT 
CAGTATCTAT GCTGGTCGCC TGTTGGGAGT AAATTAGCAT ATGTATATCA AAACAATATT 
TATTTGAAAC AAAGACCAGG AGATCCACCT TTTCAAATAA CTTATACTGG AAGAGAAAAT 
AGAATATTTA ATGGAATACC AGACTGGGTT TATGAAGAGG AAATGCTTGC CACAAAATAT 
GCTCTTTGGT GGTCTCCAGA TGGAAAATTT TTGGCATATG TAGAATTTAA TGATTCAGAT 
ATACCAATTA TTGCCTATTC TTATTATGGT GATGGACAGT ATCCTAGAAC TATAAATATT 
CCATATCCAA AGGCTGGGGC TAAGAATCCG GTTGTTCGTG TTTTTATTGT TGACACCACC 
TACCCTCACC ACGTGGGCCC AATGGAAGTG CCAGTTCCAG AAATGATAGC CTCAAGTGAC 
TATTATTTCA GCTGGCTCAC ATGGGTGTCC AGTGAACGAG TATGCTTGCA GTGGCTAAAA 
AGAGTGCAGA ATGTCTCAGT CCTGTCTATA TGTGATTTCA GGGAAGACTG GCATGCATGG 
GAATGTCCAA AGAACCAGGA GCATGTAGAA GAAAGCAGAA CAGGATGGGC TGGTGGATTC 
TTTGTTTCGA CACCAGCTTT TAGCCAGGAT GCCACTTCTT ACTACAAAAT ATTTAGCGAC 
AAGGATGGTT ACAAACATAT TCACTACATC AAAGACACTG TGGAAAATGC TATTCAAATT 
ACAAGTGGCA AGTGGGAGGC CATATATATA TTCCGCGTAA CACAGGATTC ACTGTTTTAT 
TCTAGCAATG AATTTGAAGG TTACCCTGGA AGAAGAAACA TCTACAGAAT TAGCATTGGA 
AACTCTCCTC CGAGCAAGAA GTGTGTTACT TGCCATCTAA GGAAAGAAAG GTGCCAATAT 
TACACAGCAA GTTTCAGCTA CAAAGCCAAG TACTATGCAC TCGTCTGCTA TGGCCCTGGC 
CTCCCCATTT CCACCCTCCA TGATGGCCGC ACAGACCAAG AAATACAAGT ATTAGAAGAA 
AACAAAGAAC TGGAAAATTC TCTGAGAAAT ATCCAGCTGC CTAAAGTGGA GATTAAGAAG 
CTCAAAGACG GGGGACTGAC TTTCTGGTAC AAGATGATTC TGCCTCCTCA GTTTGACAGA 
TCAAAGAAGT ACCCTTTGCT AATTCAAGTG TATGGTGGTC CTTGCAGCCA GAGTGTTAAG 
TCTGTGTTTG CTGTTAATTG GATAACTTAT CTCGCAAGTA AGGAGGGGAT AGTCATTGCC 
CTGGTAGATG GTCGGGGCAC TGCTTTCCAA GGTGACAAAT TCCTGCATGC CGTGTATCGA 
AAACTGGGTG TATATGAAGT TGAGGACCAG CTCACAGCTG TCAGAAAATT CATAGAAATG 
GGTTTCATTG ATGAAGAAAG AATAGCCATA TGGGGCTGGT CCTACGGAGG TTATGTTTCA 
TCCCTGGCCC TTGCATCTGG AACTGGTCTT TTCAAATGTG GCATAGCAGT GGCTCCAGTC 
TCCAGCTGGG AATATTACGC ATCTATCTAC TCAGAGAGAT TCATGGGCCT CCCAACAAAG 
GACGACAATC TCGAACACTA TAAAAATTCA ACTGTGATGG CAAGAGCAGA ATATTTCAGA 
AATGTAGACT ATCTTCTCAT CCACGGAACA GCAGATGATA ATGTGCACTT TCAGAACTCA 
GCACAGATTG CTAAAGCTTT GGTTAATGCA CAAGTGGATT TCCAGGCGAT GTGGTACTCT 
GACCAGAACC ATGGTATATC ATCTGGGCGC TCCCAGAATC ATTTATATAC CCACATGACG 
CACTTCCTCA AGCAATGCTT TTCTTTATCA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011237591.1
CDS	180..2135
Translation	MAENCLWSYHPGCACFSGDMHCLTSLKREFVRGYELPRPIQYLCWSPVGSKLAYVYQNNIYLKQRPGDPPFQITYTGRENRIFNGIPDWVYEEEMLATKYALWWSPDGKFLAYVEFNDSDIPIIAYSYYGDGQYPRTINIPYPKAGAKNPVVRVFIVDTTYPHHVGPMEVPVPEMIASSDYYFSWLTWVSSERVCLQWLKRVQNVSVLSICDFREDWHAWECPKNQEHVEESRTGWAGGFFVSTPAFSQDATSYYKIFSDKDGYKHIHYIKDTVENAIQITSGKWEAIYIFRVTQDSLFYSSNEFEGYPGRRNIYRISIGNSPPSKKCVTCHLRKERCQYYTASFSYKAKYYALVCYGPGLPISTLHDGRTDQEIQVLEENKELENSLRNIQLPKVEIKKLKDGGLTFWYKMILPPQFDRSKKYPLLIQVYGGPCSQSVKSVFAVNWITYLASKEGIVIALVDGRGTAFQGDKFLHAVYRKLGVYEVEDQLTAVRKFIEMGFIDEERIAIWGWSYGGYVSSLALASGTGLFKCGIAVAPVSSWEYYASIYSERFMGLPTKDDNLEHYKNSTVMARAEYFRNVDYLLIHGTADDNVHFQNSAQIAKALVNAQVDFQAMWYSDQNHGISSGRSQNHLYTHMTHFLKQCFSLSD

Target ORF information:

RefSeq Version	XM_011239289.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus fibroblast activation protein (Fap), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011239289.1

ORF Insert Sequence:

ATGGCTGAAA ACTGTCTTTG GAGTTACCAC CCTGGCTGCG CTTGCTTTAG TGGTGATATG 
CATTGTCTTA CGTCCCTCAA GAGGGAATTT GTAAGAGGAT ACGAGCTCCC TCGTCCAATT 
CAGTATCTAT GCTGGTCGCC TGTTGGGAGT AAATTAGCAT ATGTATATCA AAACAATATT 
TATTTGAAAC AAAGACCAGG AGATCCACCT TTTCAAATAA CTTATACTGG AAGAGAAAAT 
AGAATATTTA ATGGAATACC AGACTGGGTT TATGAAGAGG AAATGCTTGC CACAAAATAT 
GCTCTTTGGT GGTCTCCAGA TGGAAAATTT TTGGCATATG TAGAATTTAA TGATTCAGAT 
ATACCAATTA TTGCCTATTC TTATTATGGT GATGGACAGT ATCCTAGAAC TATAAATATT 
CCATATCCAA AGGCTGGGGC TAAGAATCCG GTTGTTCGTG TTTTTATTGT TGACACCACC 
TACCCTCACC ACGTGGGCCC AATGGAAGTG CCAGTTCCAG AAATGATAGC CTCAAGTGAC 
TATTATTTCA GCTGGCTCAC ATGGGTGTCC AGTGAACGAG TATGCTTGCA GTGGCTAAAA 
AGAGTGCAGA ATGTCTCAGT CCTGTCTATA TGTGATTTCA GGGAAGACTG GCATGCATGG 
GAATGTCCAA AGAACCAGGA GCATGTAGAA GAAAGCAGAA CAGGATGGGC TGGTGGATTC 
TTTGTTTCGA CACCAGCTTT TAGCCAGGAT GCCACTTCTT ACTACAAAAT ATTTAGCGAC 
AAGGATGGTT ACAAACATAT TCACTACATC AAAGACACTG TGGAAAATGC TATTCAAATT 
ACAAGTGGCA AGTGGGAGGC CATATATATA TTCCGCGTAA CACAGGATTC ACTGTTTTAT 
TCTAGCAATG AATTTGAAGG TTACCCTGGA AGAAGAAACA TCTACAGAAT TAGCATTGGA 
AACTCTCCTC CGAGCAAGAA GTGTGTTACT TGCCATCTAA GGAAAGAAAG GTGCCAATAT 
TACACAGCAA GTTTCAGCTA CAAAGCCAAG TACTATGCAC TCGTCTGCTA TGGCCCTGGC 
CTCCCCATTT CCACCCTCCA TGATGGCCGC ACAGACCAAG AAATACAAGT ATTAGAAGAA 
AACAAAGAAC TGGAAAATTC TCTGAGAAAT ATCCAGCTGC CTAAAGTGGA GATTAAGAAG 
CTCAAAGACG GGGGACTGAC TTTCTGGTAC AAGATGATTC TGCCTCCTCA GTTTGACAGA 
TCAAAGAAGT ACCCTTTGCT AATTCAAGTG TATGGTGGTC CTTGCAGCCA GAGTGTTAAG 
TCTGTGTTTG CTGTTAATTG GATAACTTAT CTCGCAAGTA AGGAGGGGAT AGTCATTGCC 
CTGGTAGATG GTCGGGGCAC TGCTTTCCAA GGTGACAAAT TCCTGCATGC CGTGTATCGA 
AAACTGGGTG TATATGAAGT TGAGGACCAG CTCACAGCTG TCAGAAAATT CATAGAAATG 
GGTTTCATTG ATGAAGAAAG AATAGCCATA TGGGGCTGGT CCTACGGAGG TTATGTTTCA 
TCCCTGGCCC TTGCATCTGG AACTGGTCTT TTCAAATGTG GCATAGCAGT GGCTCCAGTC 
TCCAGCTGGG AATATTACGC ATCTATCTAC TCAGAGAGAT TCATGGGCCT CCCAACAAAG 
GACGACAATC TCGAACACTA TAAAAATTCA ACTGTGATGG CAAGAGCAGA ATATTTCAGA 
AATGTAGACT ATCTTCTCAT CCACGGAACA GCAGATGATA ATGTGCACTT TCAGAACTCA 
GCACAGATTG CTAAAGCTTT GGTTAATGCA CAAGTGGATT TCCAGGCGAT GTGGTACTCT 
GACCAGAACC ATGGTATATC ATCTGGGCGC TCCCAGAATC ATTTATATAC CCACATGACG 
CACTTCCTCA AGCAATGCTT TTCTTTATCA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fap ( XM_017315512.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017171001.1 Mus musculus fibroblast activation protein (Fap), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu67719
Clone ID Related Accession (Same CDS sequence)	XM_011239289.1 , XM_017315512.1
Accession Version	XM_017315512.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	prolyl endopeptidase FAP isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAAA ACTGTCTTTG GAGTTACCAC CCTGGCTGCG CTTGCTTTAG TGGTGATATG 
CATTGTCTTA CGTCCCTCAA GAGGGAATTT GTAAGAGGAT ACGAGCTCCC TCGTCCAATT 
CAGTATCTAT GCTGGTCGCC TGTTGGGAGT AAATTAGCAT ATGTATATCA AAACAATATT 
TATTTGAAAC AAAGACCAGG AGATCCACCT TTTCAAATAA CTTATACTGG AAGAGAAAAT 
AGAATATTTA ATGGAATACC AGACTGGGTT TATGAAGAGG AAATGCTTGC CACAAAATAT 
GCTCTTTGGT GGTCTCCAGA TGGAAAATTT TTGGCATATG TAGAATTTAA TGATTCAGAT 
ATACCAATTA TTGCCTATTC TTATTATGGT GATGGACAGT ATCCTAGAAC TATAAATATT 
CCATATCCAA AGGCTGGGGC TAAGAATCCG GTTGTTCGTG TTTTTATTGT TGACACCACC 
TACCCTCACC ACGTGGGCCC AATGGAAGTG CCAGTTCCAG AAATGATAGC CTCAAGTGAC 
TATTATTTCA GCTGGCTCAC ATGGGTGTCC AGTGAACGAG TATGCTTGCA GTGGCTAAAA 
AGAGTGCAGA ATGTCTCAGT CCTGTCTATA TGTGATTTCA GGGAAGACTG GCATGCATGG 
GAATGTCCAA AGAACCAGGA GCATGTAGAA GAAAGCAGAA CAGGATGGGC TGGTGGATTC 
TTTGTTTCGA CACCAGCTTT TAGCCAGGAT GCCACTTCTT ACTACAAAAT ATTTAGCGAC 
AAGGATGGTT ACAAACATAT TCACTACATC AAAGACACTG TGGAAAATGC TATTCAAATT 
ACAAGTGGCA AGTGGGAGGC CATATATATA TTCCGCGTAA CACAGGATTC ACTGTTTTAT 
TCTAGCAATG AATTTGAAGG TTACCCTGGA AGAAGAAACA TCTACAGAAT TAGCATTGGA 
AACTCTCCTC CGAGCAAGAA GTGTGTTACT TGCCATCTAA GGAAAGAAAG GTGCCAATAT 
TACACAGCAA GTTTCAGCTA CAAAGCCAAG TACTATGCAC TCGTCTGCTA TGGCCCTGGC 
CTCCCCATTT CCACCCTCCA TGATGGCCGC ACAGACCAAG AAATACAAGT ATTAGAAGAA 
AACAAAGAAC TGGAAAATTC TCTGAGAAAT ATCCAGCTGC CTAAAGTGGA GATTAAGAAG 
CTCAAAGACG GGGGACTGAC TTTCTGGTAC AAGATGATTC TGCCTCCTCA GTTTGACAGA 
TCAAAGAAGT ACCCTTTGCT AATTCAAGTG TATGGTGGTC CTTGCAGCCA GAGTGTTAAG 
TCTGTGTTTG CTGTTAATTG GATAACTTAT CTCGCAAGTA AGGAGGGGAT AGTCATTGCC 
CTGGTAGATG GTCGGGGCAC TGCTTTCCAA GGTGACAAAT TCCTGCATGC CGTGTATCGA 
AAACTGGGTG TATATGAAGT TGAGGACCAG CTCACAGCTG TCAGAAAATT CATAGAAATG 
GGTTTCATTG ATGAAGAAAG AATAGCCATA TGGGGCTGGT CCTACGGAGG TTATGTTTCA 
TCCCTGGCCC TTGCATCTGG AACTGGTCTT TTCAAATGTG GCATAGCAGT GGCTCCAGTC 
TCCAGCTGGG AATATTACGC ATCTATCTAC TCAGAGAGAT TCATGGGCCT CCCAACAAAG 
GACGACAATC TCGAACACTA TAAAAATTCA ACTGTGATGG CAAGAGCAGA ATATTTCAGA 
AATGTAGACT ATCTTCTCAT CCACGGAACA GCAGATGATA ATGTGCACTT TCAGAACTCA 
GCACAGATTG CTAAAGCTTT GGTTAATGCA CAAGTGGATT TCCAGGCGAT GTGGTACTCT 
GACCAGAACC ATGGTATATC ATCTGGGCGC TCCCAGAATC ATTTATATAC CCACATGACG 
CACTTCCTCA AGCAATGCTT TTCTTTATCA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017171001.1
CDS	28..1983
Translation	MAENCLWSYHPGCACFSGDMHCLTSLKREFVRGYELPRPIQYLCWSPVGSKLAYVYQNNIYLKQRPGDPPFQITYTGRENRIFNGIPDWVYEEEMLATKYALWWSPDGKFLAYVEFNDSDIPIIAYSYYGDGQYPRTINIPYPKAGAKNPVVRVFIVDTTYPHHVGPMEVPVPEMIASSDYYFSWLTWVSSERVCLQWLKRVQNVSVLSICDFREDWHAWECPKNQEHVEESRTGWAGGFFVSTPAFSQDATSYYKIFSDKDGYKHIHYIKDTVENAIQITSGKWEAIYIFRVTQDSLFYSSNEFEGYPGRRNIYRISIGNSPPSKKCVTCHLRKERCQYYTASFSYKAKYYALVCYGPGLPISTLHDGRTDQEIQVLEENKELENSLRNIQLPKVEIKKLKDGGLTFWYKMILPPQFDRSKKYPLLIQVYGGPCSQSVKSVFAVNWITYLASKEGIVIALVDGRGTAFQGDKFLHAVYRKLGVYEVEDQLTAVRKFIEMGFIDEERIAIWGWSYGGYVSSLALASGTGLFKCGIAVAPVSSWEYYASIYSERFMGLPTKDDNLEHYKNSTVMARAEYFRNVDYLLIHGTADDNVHFQNSAQIAKALVNAQVDFQAMWYSDQNHGISSGRSQNHLYTHMTHFLKQCFSLSD

Target ORF information:

RefSeq Version	XM_017315512.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus fibroblast activation protein (Fap), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315512.1

ORF Insert Sequence:

ATGGCTGAAA ACTGTCTTTG GAGTTACCAC CCTGGCTGCG CTTGCTTTAG TGGTGATATG 
CATTGTCTTA CGTCCCTCAA GAGGGAATTT GTAAGAGGAT ACGAGCTCCC TCGTCCAATT 
CAGTATCTAT GCTGGTCGCC TGTTGGGAGT AAATTAGCAT ATGTATATCA AAACAATATT 
TATTTGAAAC AAAGACCAGG AGATCCACCT TTTCAAATAA CTTATACTGG AAGAGAAAAT 
AGAATATTTA ATGGAATACC AGACTGGGTT TATGAAGAGG AAATGCTTGC CACAAAATAT 
GCTCTTTGGT GGTCTCCAGA TGGAAAATTT TTGGCATATG TAGAATTTAA TGATTCAGAT 
ATACCAATTA TTGCCTATTC TTATTATGGT GATGGACAGT ATCCTAGAAC TATAAATATT 
CCATATCCAA AGGCTGGGGC TAAGAATCCG GTTGTTCGTG TTTTTATTGT TGACACCACC 
TACCCTCACC ACGTGGGCCC AATGGAAGTG CCAGTTCCAG AAATGATAGC CTCAAGTGAC 
TATTATTTCA GCTGGCTCAC ATGGGTGTCC AGTGAACGAG TATGCTTGCA GTGGCTAAAA 
AGAGTGCAGA ATGTCTCAGT CCTGTCTATA TGTGATTTCA GGGAAGACTG GCATGCATGG 
GAATGTCCAA AGAACCAGGA GCATGTAGAA GAAAGCAGAA CAGGATGGGC TGGTGGATTC 
TTTGTTTCGA CACCAGCTTT TAGCCAGGAT GCCACTTCTT ACTACAAAAT ATTTAGCGAC 
AAGGATGGTT ACAAACATAT TCACTACATC AAAGACACTG TGGAAAATGC TATTCAAATT 
ACAAGTGGCA AGTGGGAGGC CATATATATA TTCCGCGTAA CACAGGATTC ACTGTTTTAT 
TCTAGCAATG AATTTGAAGG TTACCCTGGA AGAAGAAACA TCTACAGAAT TAGCATTGGA 
AACTCTCCTC CGAGCAAGAA GTGTGTTACT TGCCATCTAA GGAAAGAAAG GTGCCAATAT 
TACACAGCAA GTTTCAGCTA CAAAGCCAAG TACTATGCAC TCGTCTGCTA TGGCCCTGGC 
CTCCCCATTT CCACCCTCCA TGATGGCCGC ACAGACCAAG AAATACAAGT ATTAGAAGAA 
AACAAAGAAC TGGAAAATTC TCTGAGAAAT ATCCAGCTGC CTAAAGTGGA GATTAAGAAG 
CTCAAAGACG GGGGACTGAC TTTCTGGTAC AAGATGATTC TGCCTCCTCA GTTTGACAGA 
TCAAAGAAGT ACCCTTTGCT AATTCAAGTG TATGGTGGTC CTTGCAGCCA GAGTGTTAAG 
TCTGTGTTTG CTGTTAATTG GATAACTTAT CTCGCAAGTA AGGAGGGGAT AGTCATTGCC 
CTGGTAGATG GTCGGGGCAC TGCTTTCCAA GGTGACAAAT TCCTGCATGC CGTGTATCGA 
AAACTGGGTG TATATGAAGT TGAGGACCAG CTCACAGCTG TCAGAAAATT CATAGAAATG 
GGTTTCATTG ATGAAGAAAG AATAGCCATA TGGGGCTGGT CCTACGGAGG TTATGTTTCA 
TCCCTGGCCC TTGCATCTGG AACTGGTCTT TTCAAATGTG GCATAGCAGT GGCTCCAGTC 
TCCAGCTGGG AATATTACGC ATCTATCTAC TCAGAGAGAT TCATGGGCCT CCCAACAAAG 
GACGACAATC TCGAACACTA TAAAAATTCA ACTGTGATGG CAAGAGCAGA ATATTTCAGA 
AATGTAGACT ATCTTCTCAT CCACGGAACA GCAGATGATA ATGTGCACTT TCAGAACTCA 
GCACAGATTG CTAAAGCTTT GGTTAATGCA CAAGTGGATT TCCAGGCGAT GTGGTACTCT 
GACCAGAACC ATGGTATATC ATCTGGGCGC TCCCAGAATC ATTTATATAC CCACATGACG 
CACTTCCTCA AGCAATGCTT TTCTTTATCA GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fap ( NM_007986.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_032012.1 Mus musculus fibroblast activation protein (Fap), mRNA.

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CloneID	OMu01178
Clone ID Related Accession (Same CDS sequence)	XM_006498746.4 , XM_006498746.3 , NM_007986.3
Accession Version	NM_007986.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	prolyl endopeptidase FAP
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL928576.9. On Apr 20, 2013 this sequence version replaced NM_007986.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: Y10007.1, BC019190.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGACAT GGCTGAAAAC TGTCTTTGGA GTTACCACCC TGGCTGCGCT TGCTTTAGTG 
GTGATATGCA TTGTCTTACG TCCCTCAAGA GTTTACAAAC CTGAAGGAAA CACAAAGAGA 
GCTCTTACCT TGAAGGATAT TTTAAATGGA ACATTCTCAT ATAAAACATA TTTTCCCAAC 
TGGATTTCAG AACAAGAATA TCTTCATCAA TCTGAGGATG ATAACATAGT ATTTTATAAT 
ATTGAAACAA GAGAATCATA TATCATTTTG AGTAATAGCA CCATGAAAAG TGTGAATGCT 
ACAGATTATG GTTTGTCACC TGATCGGCAA TTTGTGTATC TAGAAAGTGA TTATTCAAAG 
CTCTGGCGAT ATTCATACAC AGCGACATAC TACATCTACG ACCTTCAGAA TGGGGAATTT 
GTAAGAGGAT ACGAGCTCCC TCGTCCAATT CAGTATCTAT GCTGGTCGCC TGTTGGGAGT 
AAATTAGCAT ATGTATATCA AAACAATATT TATTTGAAAC AAAGACCAGG AGATCCACCT 
TTTCAAATAA CTTATACTGG AAGAGAAAAT AGAATATTTA ATGGAATACC AGACTGGGTT 
TATGAAGAGG AAATGCTTGC CACAAAATAT GCTCTTTGGT GGTCTCCAGA TGGAAAATTT 
TTGGCATATG TAGAATTTAA TGATTCAGAT ATACCAATTA TTGCCTATTC TTATTATGGT 
GATGGACAGT ATCCTAGAAC TATAAATATT CCATATCCAA AGGCTGGGGC TAAGAATCCG 
GTTGTTCGTG TTTTTATTGT TGACACCACC TACCCTCACC ACGTGGGCCC AATGGAAGTG 
CCAGTTCCAG AAATGATAGC CTCAAGTGAC TATTATTTCA GCTGGCTCAC ATGGGTGTCC 
AGTGAACGAG TATGCTTGCA GTGGCTAAAA AGAGTGCAGA ATGTCTCAGT CCTGTCTATA 
TGTGATTTCA GGGAAGACTG GCATGCATGG GAATGTCCAA AGAACCAGGA GCATGTAGAA 
GAAAGCAGAA CAGGATGGGC TGGTGGATTC TTTGTTTCGA CACCAGCTTT TAGCCAGGAT 
GCCACTTCTT ACTACAAAAT ATTTAGCGAC AAGGATGGTT ACAAACATAT TCACTACATC 
AAAGACACTG TGGAAAATGC TATTCAAATT ACAAGTGGCA AGTGGGAGGC CATATATATA 
TTCCGCGTAA CACAGGATTC ACTGTTTTAT TCTAGCAATG AATTTGAAGG TTACCCTGGA 
AGAAGAAACA TCTACAGAAT TAGCATTGGA AACTCTCCTC CGAGCAAGAA GTGTGTTACT 
TGCCATCTAA GGAAAGAAAG GTGCCAATAT TACACAGCAA GTTTCAGCTA CAAAGCCAAG 
TACTATGCAC TCGTCTGCTA TGGCCCTGGC CTCCCCATTT CCACCCTCCA TGATGGCCGC 
ACAGACCAAG AAATACAAGT ATTAGAAGAA AACAAAGAAC TGGAAAATTC TCTGAGAAAT 
ATCCAGCTGC CTAAAGTGGA GATTAAGAAG CTCAAAGACG GGGGACTGAC TTTCTGGTAC 
AAGATGATTC TGCCTCCTCA GTTTGACAGA TCAAAGAAGT ACCCTTTGCT AATTCAAGTG 
TATGGTGGTC CTTGCAGCCA GAGTGTTAAG TCTGTGTTTG CTGTTAATTG GATAACTTAT 
CTCGCAAGTA AGGAGGGGAT AGTCATTGCC CTGGTAGATG GTCGGGGCAC TGCTTTCCAA 
GGTGACAAAT TCCTGCATGC CGTGTATCGA AAACTGGGTG TATATGAAGT TGAGGACCAG 
CTCACAGCTG TCAGAAAATT CATAGAAATG GGTTTCATTG ATGAAGAAAG AATAGCCATA 
TGGGGCTGGT CCTACGGAGG TTATGTTTCA TCCCTGGCCC TTGCATCTGG AACTGGTCTT 
TTCAAATGTG GCATAGCAGT GGCTCCAGTC TCCAGCTGGG AATATTACGC ATCTATCTAC 
TCAGAGAGAT TCATGGGCCT CCCAACAAAG GACGACAATC TCGAACACTA TAAAAATTCA 
ACTGTGATGG CAAGAGCAGA ATATTTCAGA AATGTAGACT ATCTTCTCAT CCACGGAACA 
GCAGATGATA ATGTGCACTT TCAGAACTCA GCACAGATTG CTAAAGCTTT GGTTAATGCA 
CAAGTGGATT TCCAGGCGAT GTGGTACTCT GACCAGAACC ATGGTATATC ATCTGGGCGC 
TCCCAGAATC ATTTATATAC CCACATGACG CACTTCCTCA AGCAATGCTT TTCTTTATCA 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_032012.1
CDS	171..2456
Translation	MKTWLKTVFGVTTLAALALVVICIVLRPSRVYKPEGNTKRALTLKDILNGTFSYKTYFPNWISEQEYLHQSEDDNIVFYNIETRESYIILSNSTMKSVNATDYGLSPDRQFVYLESDYSKLWRYSYTATYYIYDLQNGEFVRGYELPRPIQYLCWSPVGSKLAYVYQNNIYLKQRPGDPPFQITYTGRENRIFNGIPDWVYEEEMLATKYALWWSPDGKFLAYVEFNDSDIPIIAYSYYGDGQYPRTINIPYPKAGAKNPVVRVFIVDTTYPHHVGPMEVPVPEMIASSDYYFSWLTWVSSERVCLQWLKRVQNVSVLSICDFREDWHAWECPKNQEHVEESRTGWAGGFFVSTPAFSQDATSYYKIFSDKDGYKHIHYIKDTVENAIQITSGKWEAIYIFRVTQDSLFYSSNEFEGYPGRRNIYRISIGNSPPSKKCVTCHLRKERCQYYTASFSYKAKYYALVCYGPGLPISTLHDGRTDQEIQVLEENKELENSLRNIQLPKVEIKKLKDGGLTFWYKMILPPQFDRSKKYPLLIQVYGGPCSQSVKSVFAVNWITYLASKEGIVIALVDGRGTAFQGDKFLHAVYRKLGVYEVEDQLTAVRKFIEMGFIDEERIAIWGWSYGGYVSSLALASGTGLFKCGIAVAPVSSWEYYASIYSERFMGLPTKDDNLEHYKNSTVMARAEYFRNVDYLLIHGTADDNVHFQNSAQIAKALVNAQVDFQAMWYSDQNHGISSGRSQNHLYTHMTHFLKQCFSLSD

Target ORF information:

RefSeq Version	NM_007986.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus fibroblast activation protein (Fap), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007986.3

ORF Insert Sequence:

ATGAAGACAT GGCTGAAAAC TGTCTTTGGA GTTACCACCC TGGCTGCGCT TGCTTTAGTG 
GTGATATGCA TTGTCTTACG TCCCTCAAGA GTTTACAAAC CTGAAGGAAA CACAAAGAGA 
GCTCTTACCT TGAAGGATAT TTTAAATGGA ACATTCTCAT ATAAAACATA TTTTCCCAAC 
TGGATTTCAG AACAAGAATA TCTTCATCAA TCTGAGGATG ATAACATAGT ATTTTATAAT 
ATTGAAACAA GAGAATCATA TATCATTTTG AGTAATAGCA CCATGAAAAG TGTGAATGCT 
ACAGATTATG GTTTGTCACC TGATCGGCAA TTTGTGTATC TAGAAAGTGA TTATTCAAAG 
CTCTGGCGAT ATTCATACAC AGCGACATAC TACATCTACG ACCTTCAGAA TGGGGAATTT 
GTAAGAGGAT ACGAGCTCCC TCGTCCAATT CAGTATCTAT GCTGGTCGCC TGTTGGGAGT 
AAATTAGCAT ATGTATATCA AAACAATATT TATTTGAAAC AAAGACCAGG AGATCCACCT 
TTTCAAATAA CTTATACTGG AAGAGAAAAT AGAATATTTA ATGGAATACC AGACTGGGTT 
TATGAAGAGG AAATGCTTGC CACAAAATAT GCTCTTTGGT GGTCTCCAGA TGGAAAATTT 
TTGGCATATG TAGAATTTAA TGATTCAGAT ATACCAATTA TTGCCTATTC TTATTATGGT 
GATGGACAGT ATCCTAGAAC TATAAATATT CCATATCCAA AGGCTGGGGC TAAGAATCCG 
GTTGTTCGTG TTTTTATTGT TGACACCACC TACCCTCACC ACGTGGGCCC AATGGAAGTG 
CCAGTTCCAG AAATGATAGC CTCAAGTGAC TATTATTTCA GCTGGCTCAC ATGGGTGTCC 
AGTGAACGAG TATGCTTGCA GTGGCTAAAA AGAGTGCAGA ATGTCTCAGT CCTGTCTATA 
TGTGATTTCA GGGAAGACTG GCATGCATGG GAATGTCCAA AGAACCAGGA GCATGTAGAA 
GAAAGCAGAA CAGGATGGGC TGGTGGATTC TTTGTTTCGA CACCAGCTTT TAGCCAGGAT 
GCCACTTCTT ACTACAAAAT ATTTAGCGAC AAGGATGGTT ACAAACATAT TCACTACATC 
AAAGACACTG TGGAAAATGC TATTCAAATT ACAAGTGGCA AGTGGGAGGC CATATATATA 
TTCCGCGTAA CACAGGATTC ACTGTTTTAT TCTAGCAATG AATTTGAAGG TTACCCTGGA 
AGAAGAAACA TCTACAGAAT TAGCATTGGA AACTCTCCTC CGAGCAAGAA GTGTGTTACT 
TGCCATCTAA GGAAAGAAAG GTGCCAATAT TACACAGCAA GTTTCAGCTA CAAAGCCAAG 
TACTATGCAC TCGTCTGCTA TGGCCCTGGC CTCCCCATTT CCACCCTCCA TGATGGCCGC 
ACAGACCAAG AAATACAAGT ATTAGAAGAA AACAAAGAAC TGGAAAATTC TCTGAGAAAT 
ATCCAGCTGC CTAAAGTGGA GATTAAGAAG CTCAAAGACG GGGGACTGAC TTTCTGGTAC 
AAGATGATTC TGCCTCCTCA GTTTGACAGA TCAAAGAAGT ACCCTTTGCT AATTCAAGTG 
TATGGTGGTC CTTGCAGCCA GAGTGTTAAG TCTGTGTTTG CTGTTAATTG GATAACTTAT 
CTCGCAAGTA AGGAGGGGAT AGTCATTGCC CTGGTAGATG GTCGGGGCAC TGCTTTCCAA 
GGTGACAAAT TCCTGCATGC CGTGTATCGA AAACTGGGTG TATATGAAGT TGAGGACCAG 
CTCACAGCTG TCAGAAAATT CATAGAAATG GGTTTCATTG ATGAAGAAAG AATAGCCATA 
TGGGGCTGGT CCTACGGAGG TTATGTTTCA TCCCTGGCCC TTGCATCTGG AACTGGTCTT 
TTCAAATGTG GCATAGCAGT GGCTCCAGTC TCCAGCTGGG AATATTACGC ATCTATCTAC 
TCAGAGAGAT TCATGGGCCT CCCAACAAAG GACGACAATC TCGAACACTA TAAAAATTCA 
ACTGTGATGG CAAGAGCAGA ATATTTCAGA AATGTAGACT ATCTTCTCAT CCACGGAACA 
GCAGATGATA ATGTGCACTT TCAGAACTCA GCACAGATTG CTAAAGCTTT GGTTAATGCA 
CAAGTGGATT TCCAGGCGAT GTGGTACTCT GACCAGAACC ATGGTATATC ATCTGGGCGC 
TCCCAGAATC ATTTATATAC CCACATGACG CACTTCCTCA AGCAATGCTT TTCTTTATCA 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fap ( XM_006498746.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498809.1 Mus musculus fibroblast activation protein (Fap), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu01178
Clone ID Related Accession (Same CDS sequence)	XM_006498746.4 , XM_006498746.3 , NM_007986.3
Accession Version	XM_006498746.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	prolyl endopeptidase FAP isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006498746.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACAT GGCTGAAAAC TGTCTTTGGA GTTACCACCC TGGCTGCGCT TGCTTTAGTG 
GTGATATGCA TTGTCTTACG TCCCTCAAGA GTTTACAAAC CTGAAGGAAA CACAAAGAGA 
GCTCTTACCT TGAAGGATAT TTTAAATGGA ACATTCTCAT ATAAAACATA TTTTCCCAAC 
TGGATTTCAG AACAAGAATA TCTTCATCAA TCTGAGGATG ATAACATAGT ATTTTATAAT 
ATTGAAACAA GAGAATCATA TATCATTTTG AGTAATAGCA CCATGAAAAG TGTGAATGCT 
ACAGATTATG GTTTGTCACC TGATCGGCAA TTTGTGTATC TAGAAAGTGA TTATTCAAAG 
CTCTGGCGAT ATTCATACAC AGCGACATAC TACATCTACG ACCTTCAGAA TGGGGAATTT 
GTAAGAGGAT ACGAGCTCCC TCGTCCAATT CAGTATCTAT GCTGGTCGCC TGTTGGGAGT 
AAATTAGCAT ATGTATATCA AAACAATATT TATTTGAAAC AAAGACCAGG AGATCCACCT 
TTTCAAATAA CTTATACTGG AAGAGAAAAT AGAATATTTA ATGGAATACC AGACTGGGTT 
TATGAAGAGG AAATGCTTGC CACAAAATAT GCTCTTTGGT GGTCTCCAGA TGGAAAATTT 
TTGGCATATG TAGAATTTAA TGATTCAGAT ATACCAATTA TTGCCTATTC TTATTATGGT 
GATGGACAGT ATCCTAGAAC TATAAATATT CCATATCCAA AGGCTGGGGC TAAGAATCCG 
GTTGTTCGTG TTTTTATTGT TGACACCACC TACCCTCACC ACGTGGGCCC AATGGAAGTG 
CCAGTTCCAG AAATGATAGC CTCAAGTGAC TATTATTTCA GCTGGCTCAC ATGGGTGTCC 
AGTGAACGAG TATGCTTGCA GTGGCTAAAA AGAGTGCAGA ATGTCTCAGT CCTGTCTATA 
TGTGATTTCA GGGAAGACTG GCATGCATGG GAATGTCCAA AGAACCAGGA GCATGTAGAA 
GAAAGCAGAA CAGGATGGGC TGGTGGATTC TTTGTTTCGA CACCAGCTTT TAGCCAGGAT 
GCCACTTCTT ACTACAAAAT ATTTAGCGAC AAGGATGGTT ACAAACATAT TCACTACATC 
AAAGACACTG TGGAAAATGC TATTCAAATT ACAAGTGGCA AGTGGGAGGC CATATATATA 
TTCCGCGTAA CACAGGATTC ACTGTTTTAT TCTAGCAATG AATTTGAAGG TTACCCTGGA 
AGAAGAAACA TCTACAGAAT TAGCATTGGA AACTCTCCTC CGAGCAAGAA GTGTGTTACT 
TGCCATCTAA GGAAAGAAAG GTGCCAATAT TACACAGCAA GTTTCAGCTA CAAAGCCAAG 
TACTATGCAC TCGTCTGCTA TGGCCCTGGC CTCCCCATTT CCACCCTCCA TGATGGCCGC 
ACAGACCAAG AAATACAAGT ATTAGAAGAA AACAAAGAAC TGGAAAATTC TCTGAGAAAT 
ATCCAGCTGC CTAAAGTGGA GATTAAGAAG CTCAAAGACG GGGGACTGAC TTTCTGGTAC 
AAGATGATTC TGCCTCCTCA GTTTGACAGA TCAAAGAAGT ACCCTTTGCT AATTCAAGTG 
TATGGTGGTC CTTGCAGCCA GAGTGTTAAG TCTGTGTTTG CTGTTAATTG GATAACTTAT 
CTCGCAAGTA AGGAGGGGAT AGTCATTGCC CTGGTAGATG GTCGGGGCAC TGCTTTCCAA 
GGTGACAAAT TCCTGCATGC CGTGTATCGA AAACTGGGTG TATATGAAGT TGAGGACCAG 
CTCACAGCTG TCAGAAAATT CATAGAAATG GGTTTCATTG ATGAAGAAAG AATAGCCATA 
TGGGGCTGGT CCTACGGAGG TTATGTTTCA TCCCTGGCCC TTGCATCTGG AACTGGTCTT 
TTCAAATGTG GCATAGCAGT GGCTCCAGTC TCCAGCTGGG AATATTACGC ATCTATCTAC 
TCAGAGAGAT TCATGGGCCT CCCAACAAAG GACGACAATC TCGAACACTA TAAAAATTCA 
ACTGTGATGG CAAGAGCAGA ATATTTCAGA AATGTAGACT ATCTTCTCAT CCACGGAACA 
GCAGATGATA ATGTGCACTT TCAGAACTCA GCACAGATTG CTAAAGCTTT GGTTAATGCA 
CAAGTGGATT TCCAGGCGAT GTGGTACTCT GACCAGAACC ATGGTATATC ATCTGGGCGC 
TCCCAGAATC ATTTATATAC CCACATGACG CACTTCCTCA AGCAATGCTT TTCTTTATCA 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498809.1
CDS	162..2447
Translation	MKTWLKTVFGVTTLAALALVVICIVLRPSRVYKPEGNTKRALTLKDILNGTFSYKTYFPNWISEQEYLHQSEDDNIVFYNIETRESYIILSNSTMKSVNATDYGLSPDRQFVYLESDYSKLWRYSYTATYYIYDLQNGEFVRGYELPRPIQYLCWSPVGSKLAYVYQNNIYLKQRPGDPPFQITYTGRENRIFNGIPDWVYEEEMLATKYALWWSPDGKFLAYVEFNDSDIPIIAYSYYGDGQYPRTINIPYPKAGAKNPVVRVFIVDTTYPHHVGPMEVPVPEMIASSDYYFSWLTWVSSERVCLQWLKRVQNVSVLSICDFREDWHAWECPKNQEHVEESRTGWAGGFFVSTPAFSQDATSYYKIFSDKDGYKHIHYIKDTVENAIQITSGKWEAIYIFRVTQDSLFYSSNEFEGYPGRRNIYRISIGNSPPSKKCVTCHLRKERCQYYTASFSYKAKYYALVCYGPGLPISTLHDGRTDQEIQVLEENKELENSLRNIQLPKVEIKKLKDGGLTFWYKMILPPQFDRSKKYPLLIQVYGGPCSQSVKSVFAVNWITYLASKEGIVIALVDGRGTAFQGDKFLHAVYRKLGVYEVEDQLTAVRKFIEMGFIDEERIAIWGWSYGGYVSSLALASGTGLFKCGIAVAPVSSWEYYASIYSERFMGLPTKDDNLEHYKNSTVMARAEYFRNVDYLLIHGTADDNVHFQNSAQIAKALVNAQVDFQAMWYSDQNHGISSGRSQNHLYTHMTHFLKQCFSLSD

Target ORF information:

RefSeq Version	XM_006498746.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus fibroblast activation protein (Fap), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498746.3

ORF Insert Sequence:

ATGAAGACAT GGCTGAAAAC TGTCTTTGGA GTTACCACCC TGGCTGCGCT TGCTTTAGTG 
GTGATATGCA TTGTCTTACG TCCCTCAAGA GTTTACAAAC CTGAAGGAAA CACAAAGAGA 
GCTCTTACCT TGAAGGATAT TTTAAATGGA ACATTCTCAT ATAAAACATA TTTTCCCAAC 
TGGATTTCAG AACAAGAATA TCTTCATCAA TCTGAGGATG ATAACATAGT ATTTTATAAT 
ATTGAAACAA GAGAATCATA TATCATTTTG AGTAATAGCA CCATGAAAAG TGTGAATGCT 
ACAGATTATG GTTTGTCACC TGATCGGCAA TTTGTGTATC TAGAAAGTGA TTATTCAAAG 
CTCTGGCGAT ATTCATACAC AGCGACATAC TACATCTACG ACCTTCAGAA TGGGGAATTT 
GTAAGAGGAT ACGAGCTCCC TCGTCCAATT CAGTATCTAT GCTGGTCGCC TGTTGGGAGT 
AAATTAGCAT ATGTATATCA AAACAATATT TATTTGAAAC AAAGACCAGG AGATCCACCT 
TTTCAAATAA CTTATACTGG AAGAGAAAAT AGAATATTTA ATGGAATACC AGACTGGGTT 
TATGAAGAGG AAATGCTTGC CACAAAATAT GCTCTTTGGT GGTCTCCAGA TGGAAAATTT 
TTGGCATATG TAGAATTTAA TGATTCAGAT ATACCAATTA TTGCCTATTC TTATTATGGT 
GATGGACAGT ATCCTAGAAC TATAAATATT CCATATCCAA AGGCTGGGGC TAAGAATCCG 
GTTGTTCGTG TTTTTATTGT TGACACCACC TACCCTCACC ACGTGGGCCC AATGGAAGTG 
CCAGTTCCAG AAATGATAGC CTCAAGTGAC TATTATTTCA GCTGGCTCAC ATGGGTGTCC 
AGTGAACGAG TATGCTTGCA GTGGCTAAAA AGAGTGCAGA ATGTCTCAGT CCTGTCTATA 
TGTGATTTCA GGGAAGACTG GCATGCATGG GAATGTCCAA AGAACCAGGA GCATGTAGAA 
GAAAGCAGAA CAGGATGGGC TGGTGGATTC TTTGTTTCGA CACCAGCTTT TAGCCAGGAT 
GCCACTTCTT ACTACAAAAT ATTTAGCGAC AAGGATGGTT ACAAACATAT TCACTACATC 
AAAGACACTG TGGAAAATGC TATTCAAATT ACAAGTGGCA AGTGGGAGGC CATATATATA 
TTCCGCGTAA CACAGGATTC ACTGTTTTAT TCTAGCAATG AATTTGAAGG TTACCCTGGA 
AGAAGAAACA TCTACAGAAT TAGCATTGGA AACTCTCCTC CGAGCAAGAA GTGTGTTACT 
TGCCATCTAA GGAAAGAAAG GTGCCAATAT TACACAGCAA GTTTCAGCTA CAAAGCCAAG 
TACTATGCAC TCGTCTGCTA TGGCCCTGGC CTCCCCATTT CCACCCTCCA TGATGGCCGC 
ACAGACCAAG AAATACAAGT ATTAGAAGAA AACAAAGAAC TGGAAAATTC TCTGAGAAAT 
ATCCAGCTGC CTAAAGTGGA GATTAAGAAG CTCAAAGACG GGGGACTGAC TTTCTGGTAC 
AAGATGATTC TGCCTCCTCA GTTTGACAGA TCAAAGAAGT ACCCTTTGCT AATTCAAGTG 
TATGGTGGTC CTTGCAGCCA GAGTGTTAAG TCTGTGTTTG CTGTTAATTG GATAACTTAT 
CTCGCAAGTA AGGAGGGGAT AGTCATTGCC CTGGTAGATG GTCGGGGCAC TGCTTTCCAA 
GGTGACAAAT TCCTGCATGC CGTGTATCGA AAACTGGGTG TATATGAAGT TGAGGACCAG 
CTCACAGCTG TCAGAAAATT CATAGAAATG GGTTTCATTG ATGAAGAAAG AATAGCCATA 
TGGGGCTGGT CCTACGGAGG TTATGTTTCA TCCCTGGCCC TTGCATCTGG AACTGGTCTT 
TTCAAATGTG GCATAGCAGT GGCTCCAGTC TCCAGCTGGG AATATTACGC ATCTATCTAC 
TCAGAGAGAT TCATGGGCCT CCCAACAAAG GACGACAATC TCGAACACTA TAAAAATTCA 
ACTGTGATGG CAAGAGCAGA ATATTTCAGA AATGTAGACT ATCTTCTCAT CCACGGAACA 
GCAGATGATA ATGTGCACTT TCAGAACTCA GCACAGATTG CTAAAGCTTT GGTTAATGCA 
CAAGTGGATT TCCAGGCGAT GTGGTACTCT GACCAGAACC ATGGTATATC ATCTGGGCGC 
TCCCAGAATC ATTTATATAC CCACATGACG CACTTCCTCA AGCAATGCTT TTCTTTATCA 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fap ( XM_006498746.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498809.1 Mus musculus fibroblast activation protein (Fap), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu01178
Clone ID Related Accession (Same CDS sequence)	XM_006498746.4 , XM_006498746.3 , NM_007986.3
Accession Version	XM_006498746.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	prolyl endopeptidase FAP isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006498746.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACAT GGCTGAAAAC TGTCTTTGGA GTTACCACCC TGGCTGCGCT TGCTTTAGTG 
GTGATATGCA TTGTCTTACG TCCCTCAAGA GTTTACAAAC CTGAAGGAAA CACAAAGAGA 
GCTCTTACCT TGAAGGATAT TTTAAATGGA ACATTCTCAT ATAAAACATA TTTTCCCAAC 
TGGATTTCAG AACAAGAATA TCTTCATCAA TCTGAGGATG ATAACATAGT ATTTTATAAT 
ATTGAAACAA GAGAATCATA TATCATTTTG AGTAATAGCA CCATGAAAAG TGTGAATGCT 
ACAGATTATG GTTTGTCACC TGATCGGCAA TTTGTGTATC TAGAAAGTGA TTATTCAAAG 
CTCTGGCGAT ATTCATACAC AGCGACATAC TACATCTACG ACCTTCAGAA TGGGGAATTT 
GTAAGAGGAT ACGAGCTCCC TCGTCCAATT CAGTATCTAT GCTGGTCGCC TGTTGGGAGT 
AAATTAGCAT ATGTATATCA AAACAATATT TATTTGAAAC AAAGACCAGG AGATCCACCT 
TTTCAAATAA CTTATACTGG AAGAGAAAAT AGAATATTTA ATGGAATACC AGACTGGGTT 
TATGAAGAGG AAATGCTTGC CACAAAATAT GCTCTTTGGT GGTCTCCAGA TGGAAAATTT 
TTGGCATATG TAGAATTTAA TGATTCAGAT ATACCAATTA TTGCCTATTC TTATTATGGT 
GATGGACAGT ATCCTAGAAC TATAAATATT CCATATCCAA AGGCTGGGGC TAAGAATCCG 
GTTGTTCGTG TTTTTATTGT TGACACCACC TACCCTCACC ACGTGGGCCC AATGGAAGTG 
CCAGTTCCAG AAATGATAGC CTCAAGTGAC TATTATTTCA GCTGGCTCAC ATGGGTGTCC 
AGTGAACGAG TATGCTTGCA GTGGCTAAAA AGAGTGCAGA ATGTCTCAGT CCTGTCTATA 
TGTGATTTCA GGGAAGACTG GCATGCATGG GAATGTCCAA AGAACCAGGA GCATGTAGAA 
GAAAGCAGAA CAGGATGGGC TGGTGGATTC TTTGTTTCGA CACCAGCTTT TAGCCAGGAT 
GCCACTTCTT ACTACAAAAT ATTTAGCGAC AAGGATGGTT ACAAACATAT TCACTACATC 
AAAGACACTG TGGAAAATGC TATTCAAATT ACAAGTGGCA AGTGGGAGGC CATATATATA 
TTCCGCGTAA CACAGGATTC ACTGTTTTAT TCTAGCAATG AATTTGAAGG TTACCCTGGA 
AGAAGAAACA TCTACAGAAT TAGCATTGGA AACTCTCCTC CGAGCAAGAA GTGTGTTACT 
TGCCATCTAA GGAAAGAAAG GTGCCAATAT TACACAGCAA GTTTCAGCTA CAAAGCCAAG 
TACTATGCAC TCGTCTGCTA TGGCCCTGGC CTCCCCATTT CCACCCTCCA TGATGGCCGC 
ACAGACCAAG AAATACAAGT ATTAGAAGAA AACAAAGAAC TGGAAAATTC TCTGAGAAAT 
ATCCAGCTGC CTAAAGTGGA GATTAAGAAG CTCAAAGACG GGGGACTGAC TTTCTGGTAC 
AAGATGATTC TGCCTCCTCA GTTTGACAGA TCAAAGAAGT ACCCTTTGCT AATTCAAGTG 
TATGGTGGTC CTTGCAGCCA GAGTGTTAAG TCTGTGTTTG CTGTTAATTG GATAACTTAT 
CTCGCAAGTA AGGAGGGGAT AGTCATTGCC CTGGTAGATG GTCGGGGCAC TGCTTTCCAA 
GGTGACAAAT TCCTGCATGC CGTGTATCGA AAACTGGGTG TATATGAAGT TGAGGACCAG 
CTCACAGCTG TCAGAAAATT CATAGAAATG GGTTTCATTG ATGAAGAAAG AATAGCCATA 
TGGGGCTGGT CCTACGGAGG TTATGTTTCA TCCCTGGCCC TTGCATCTGG AACTGGTCTT 
TTCAAATGTG GCATAGCAGT GGCTCCAGTC TCCAGCTGGG AATATTACGC ATCTATCTAC 
TCAGAGAGAT TCATGGGCCT CCCAACAAAG GACGACAATC TCGAACACTA TAAAAATTCA 
ACTGTGATGG CAAGAGCAGA ATATTTCAGA AATGTAGACT ATCTTCTCAT CCACGGAACA 
GCAGATGATA ATGTGCACTT TCAGAACTCA GCACAGATTG CTAAAGCTTT GGTTAATGCA 
CAAGTGGATT TCCAGGCGAT GTGGTACTCT GACCAGAACC ATGGTATATC ATCTGGGCGC 
TCCCAGAATC ATTTATATAC CCACATGACG CACTTCCTCA AGCAATGCTT TTCTTTATCA 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498809.1
CDS	161..2446
Translation	MKTWLKTVFGVTTLAALALVVICIVLRPSRVYKPEGNTKRALTLKDILNGTFSYKTYFPNWISEQEYLHQSEDDNIVFYNIETRESYIILSNSTMKSVNATDYGLSPDRQFVYLESDYSKLWRYSYTATYYIYDLQNGEFVRGYELPRPIQYLCWSPVGSKLAYVYQNNIYLKQRPGDPPFQITYTGRENRIFNGIPDWVYEEEMLATKYALWWSPDGKFLAYVEFNDSDIPIIAYSYYGDGQYPRTINIPYPKAGAKNPVVRVFIVDTTYPHHVGPMEVPVPEMIASSDYYFSWLTWVSSERVCLQWLKRVQNVSVLSICDFREDWHAWECPKNQEHVEESRTGWAGGFFVSTPAFSQDATSYYKIFSDKDGYKHIHYIKDTVENAIQITSGKWEAIYIFRVTQDSLFYSSNEFEGYPGRRNIYRISIGNSPPSKKCVTCHLRKERCQYYTASFSYKAKYYALVCYGPGLPISTLHDGRTDQEIQVLEENKELENSLRNIQLPKVEIKKLKDGGLTFWYKMILPPQFDRSKKYPLLIQVYGGPCSQSVKSVFAVNWITYLASKEGIVIALVDGRGTAFQGDKFLHAVYRKLGVYEVEDQLTAVRKFIEMGFIDEERIAIWGWSYGGYVSSLALASGTGLFKCGIAVAPVSSWEYYASIYSERFMGLPTKDDNLEHYKNSTVMARAEYFRNVDYLLIHGTADDNVHFQNSAQIAKALVNAQVDFQAMWYSDQNHGISSGRSQNHLYTHMTHFLKQCFSLSD

Target ORF information:

RefSeq Version	XM_006498746.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus fibroblast activation protein (Fap), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498746.4

ORF Insert Sequence:

ATGAAGACAT GGCTGAAAAC TGTCTTTGGA GTTACCACCC TGGCTGCGCT TGCTTTAGTG 
GTGATATGCA TTGTCTTACG TCCCTCAAGA GTTTACAAAC CTGAAGGAAA CACAAAGAGA 
GCTCTTACCT TGAAGGATAT TTTAAATGGA ACATTCTCAT ATAAAACATA TTTTCCCAAC 
TGGATTTCAG AACAAGAATA TCTTCATCAA TCTGAGGATG ATAACATAGT ATTTTATAAT 
ATTGAAACAA GAGAATCATA TATCATTTTG AGTAATAGCA CCATGAAAAG TGTGAATGCT 
ACAGATTATG GTTTGTCACC TGATCGGCAA TTTGTGTATC TAGAAAGTGA TTATTCAAAG 
CTCTGGCGAT ATTCATACAC AGCGACATAC TACATCTACG ACCTTCAGAA TGGGGAATTT 
GTAAGAGGAT ACGAGCTCCC TCGTCCAATT CAGTATCTAT GCTGGTCGCC TGTTGGGAGT 
AAATTAGCAT ATGTATATCA AAACAATATT TATTTGAAAC AAAGACCAGG AGATCCACCT 
TTTCAAATAA CTTATACTGG AAGAGAAAAT AGAATATTTA ATGGAATACC AGACTGGGTT 
TATGAAGAGG AAATGCTTGC CACAAAATAT GCTCTTTGGT GGTCTCCAGA TGGAAAATTT 
TTGGCATATG TAGAATTTAA TGATTCAGAT ATACCAATTA TTGCCTATTC TTATTATGGT 
GATGGACAGT ATCCTAGAAC TATAAATATT CCATATCCAA AGGCTGGGGC TAAGAATCCG 
GTTGTTCGTG TTTTTATTGT TGACACCACC TACCCTCACC ACGTGGGCCC AATGGAAGTG 
CCAGTTCCAG AAATGATAGC CTCAAGTGAC TATTATTTCA GCTGGCTCAC ATGGGTGTCC 
AGTGAACGAG TATGCTTGCA GTGGCTAAAA AGAGTGCAGA ATGTCTCAGT CCTGTCTATA 
TGTGATTTCA GGGAAGACTG GCATGCATGG GAATGTCCAA AGAACCAGGA GCATGTAGAA 
GAAAGCAGAA CAGGATGGGC TGGTGGATTC TTTGTTTCGA CACCAGCTTT TAGCCAGGAT 
GCCACTTCTT ACTACAAAAT ATTTAGCGAC AAGGATGGTT ACAAACATAT TCACTACATC 
AAAGACACTG TGGAAAATGC TATTCAAATT ACAAGTGGCA AGTGGGAGGC CATATATATA 
TTCCGCGTAA CACAGGATTC ACTGTTTTAT TCTAGCAATG AATTTGAAGG TTACCCTGGA 
AGAAGAAACA TCTACAGAAT TAGCATTGGA AACTCTCCTC CGAGCAAGAA GTGTGTTACT 
TGCCATCTAA GGAAAGAAAG GTGCCAATAT TACACAGCAA GTTTCAGCTA CAAAGCCAAG 
TACTATGCAC TCGTCTGCTA TGGCCCTGGC CTCCCCATTT CCACCCTCCA TGATGGCCGC 
ACAGACCAAG AAATACAAGT ATTAGAAGAA AACAAAGAAC TGGAAAATTC TCTGAGAAAT 
ATCCAGCTGC CTAAAGTGGA GATTAAGAAG CTCAAAGACG GGGGACTGAC TTTCTGGTAC 
AAGATGATTC TGCCTCCTCA GTTTGACAGA TCAAAGAAGT ACCCTTTGCT AATTCAAGTG 
TATGGTGGTC CTTGCAGCCA GAGTGTTAAG TCTGTGTTTG CTGTTAATTG GATAACTTAT 
CTCGCAAGTA AGGAGGGGAT AGTCATTGCC CTGGTAGATG GTCGGGGCAC TGCTTTCCAA 
GGTGACAAAT TCCTGCATGC CGTGTATCGA AAACTGGGTG TATATGAAGT TGAGGACCAG 
CTCACAGCTG TCAGAAAATT CATAGAAATG GGTTTCATTG ATGAAGAAAG AATAGCCATA 
TGGGGCTGGT CCTACGGAGG TTATGTTTCA TCCCTGGCCC TTGCATCTGG AACTGGTCTT 
TTCAAATGTG GCATAGCAGT GGCTCCAGTC TCCAGCTGGG AATATTACGC ATCTATCTAC 
TCAGAGAGAT TCATGGGCCT CCCAACAAAG GACGACAATC TCGAACACTA TAAAAATTCA 
ACTGTGATGG CAAGAGCAGA ATATTTCAGA AATGTAGACT ATCTTCTCAT CCACGGAACA 
GCAGATGATA ATGTGCACTT TCAGAACTCA GCACAGATTG CTAAAGCTTT GGTTAATGCA 
CAAGTGGATT TCCAGGCGAT GTGGTACTCT GACCAGAACC ATGGTATATC ATCTGGGCGC 
TCCCAGAATC ATTTATATAC CCACATGACG CACTTCCTCA AGCAATGCTT TTCTTTATCA 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fap ( XM_017315507.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017170996.1 Mus musculus fibroblast activation protein (Fap), transcript variant X1, mRNA.

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CloneID	OMu80065
Clone ID Related Accession (Same CDS sequence)	XM_017315507.1
Accession Version	XM_017315507.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	prolyl endopeptidase FAP isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAGAG TTTTCTATTC TTTAGAGAAG ACATGGCTGA AAACTGTCTT TGGAGTTACC 
ACCCTGGCTG CGCTTGCTTT AGTGGTGATA TGCATTGTCT TACGTCCCTC AAGAGTTTAC 
AAACCTGAAG GAAACACAAA GAGAGCTCTT ACCTTGAAGG ATATTTTAAA TGGAACATTC 
TCATATAAAA CATATTTTCC CAACTGGATT TCAGAACAAG AATATCTTCA TCAATCTGAG 
GATGATAACA TAGTATTTTA TAATATTGAA ACAAGAGAAT CATATATCAT TTTGAGTAAT 
AGCACCATGA AAAGTGTGAA TGCTACAGAT TATGGTTTGT CACCTGATCG GCAATTTGTG 
TATCTAGAAA GTGATTATTC AAAGCTCTGG CGATATTCAT ACACAGCGAC ATACTACATC 
TACGACCTTC AGAATGGGGA ATTTGTAAGA GGATACGAGC TCCCTCGTCC AATTCAGTAT 
CTATGCTGGT CGCCTGTTGG GAGTAAATTA GCATATGTAT ATCAAAACAA TATTTATTTG 
AAACAAAGAC CAGGAGATCC ACCTTTTCAA ATAACTTATA CTGGAAGAGA AAATAGAATA 
TTTAATGGAA TACCAGACTG GGTTTATGAA GAGGAAATGC TTGCCACAAA ATATGCTCTT 
TGGTGGTCTC CAGATGGAAA ATTTTTGGCA TATGTAGAAT TTAATGATTC AGATATACCA 
ATTATTGCCT ATTCTTATTA TGGTGATGGA CAGTATCCTA GAACTATAAA TATTCCATAT 
CCAAAGGCTG GGGCTAAGAA TCCGGTTGTT CGTGTTTTTA TTGTTGACAC CACCTACCCT 
CACCACGTGG GCCCAATGGA AGTGCCAGTT CCAGAAATGA TAGCCTCAAG TGACTATTAT 
TTCAGCTGGC TCACATGGGT GTCCAGTGAA CGAGTATGCT TGCAGTGGCT AAAAAGAGTG 
CAGAATGTCT CAGTCCTGTC TATATGTGAT TTCAGGGAAG ACTGGCATGC ATGGGAATGT 
CCAAAGAACC AGGAGCATGT AGAAGAAAGC AGAACAGGAT GGGCTGGTGG ATTCTTTGTT 
TCGACACCAG CTTTTAGCCA GGATGCCACT TCTTACTACA AAATATTTAG CGACAAGGAT 
GGTTACAAAC ATATTCACTA CATCAAAGAC ACTGTGGAAA ATGCTATTCA AATTACAAGT 
GGCAAGTGGG AGGCCATATA TATATTCCGC GTAACACAGG ATTCACTGTT TTATTCTAGC 
AATGAATTTG AAGGTTACCC TGGAAGAAGA AACATCTACA GAATTAGCAT TGGAAACTCT 
CCTCCGAGCA AGAAGTGTGT TACTTGCCAT CTAAGGAAAG AAAGGTGCCA ATATTACACA 
GCAAGTTTCA GCTACAAAGC CAAGTACTAT GCACTCGTCT GCTATGGCCC TGGCCTCCCC 
ATTTCCACCC TCCATGATGG CCGCACAGAC CAAGAAATAC AAGTATTAGA AGAAAACAAA 
GAACTGGAAA ATTCTCTGAG AAATATCCAG CTGCCTAAAG TGGAGATTAA GAAGCTCAAA 
GACGGGGGAC TGACTTTCTG GTACAAGATG ATTCTGCCTC CTCAGTTTGA CAGATCAAAG 
AAGTACCCTT TGCTAATTCA AGTGTATGGT GGTCCTTGCA GCCAGAGTGT TAAGTCTGTG 
TTTGCTGTTA ATTGGATAAC TTATCTCGCA AGTAAGGAGG GGATAGTCAT TGCCCTGGTA 
GATGGTCGGG GCACTGCTTT CCAAGGTGAC AAATTCCTGC ATGCCGTGTA TCGAAAACTG 
GGTGTATATG AAGTTGAGGA CCAGCTCACA GCTGTCAGAA AATTCATAGA AATGGGTTTC 
ATTGATGAAG AAAGAATAGC CATATGGGGC TGGTCCTACG GAGGTTATGT TTCATCCCTG 
GCCCTTGCAT CTGGAACTGG TCTTTTCAAA TGTGGCATAG CAGTGGCTCC AGTCTCCAGC 
TGGGAATATT ACGCATCTAT CTACTCAGAG AGATTCATGG GCCTCCCAAC AAAGGACGAC 
AATCTCGAAC ACTATAAAAA TTCAACTGTG ATGGCAAGAG CAGAATATTT CAGAAATGTA 
GACTATCTTC TCATCCACGG AACAGCAGAT GATAATGTGC ACTTTCAGAA CTCAGCACAG 
ATTGCTAAAG CTTTGGTTAA TGCACAAGTG GATTTCCAGG CGATGTGGTA CTCTGACCAG 
AACCATGGTA TATCATCTGG GCGCTCCCAG AATCATTTAT ATACCCACAT GACGCACTTC 
CTCAAGCAAT GCTTTTCTTT ATCAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017170996.1
CDS	15..2324
Translation	MVRVFYSLEKTWLKTVFGVTTLAALALVVICIVLRPSRVYKPEGNTKRALTLKDILNGTFSYKTYFPNWISEQEYLHQSEDDNIVFYNIETRESYIILSNSTMKSVNATDYGLSPDRQFVYLESDYSKLWRYSYTATYYIYDLQNGEFVRGYELPRPIQYLCWSPVGSKLAYVYQNNIYLKQRPGDPPFQITYTGRENRIFNGIPDWVYEEEMLATKYALWWSPDGKFLAYVEFNDSDIPIIAYSYYGDGQYPRTINIPYPKAGAKNPVVRVFIVDTTYPHHVGPMEVPVPEMIASSDYYFSWLTWVSSERVCLQWLKRVQNVSVLSICDFREDWHAWECPKNQEHVEESRTGWAGGFFVSTPAFSQDATSYYKIFSDKDGYKHIHYIKDTVENAIQITSGKWEAIYIFRVTQDSLFYSSNEFEGYPGRRNIYRISIGNSPPSKKCVTCHLRKERCQYYTASFSYKAKYYALVCYGPGLPISTLHDGRTDQEIQVLEENKELENSLRNIQLPKVEIKKLKDGGLTFWYKMILPPQFDRSKKYPLLIQVYGGPCSQSVKSVFAVNWITYLASKEGIVIALVDGRGTAFQGDKFLHAVYRKLGVYEVEDQLTAVRKFIEMGFIDEERIAIWGWSYGGYVSSLALASGTGLFKCGIAVAPVSSWEYYASIYSERFMGLPTKDDNLEHYKNSTVMARAEYFRNVDYLLIHGTADDNVHFQNSAQIAKALVNAQVDFQAMWYSDQNHGISSGRSQNHLYTHMTHFLKQCFSLSD

Target ORF information:

RefSeq Version	XM_017315507.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus fibroblast activation protein (Fap), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017315507.1

ORF Insert Sequence:

ATGGTCAGAG TTTTCTATTC TTTAGAGAAG ACATGGCTGA AAACTGTCTT TGGAGTTACC 
ACCCTGGCTG CGCTTGCTTT AGTGGTGATA TGCATTGTCT TACGTCCCTC AAGAGTTTAC 
AAACCTGAAG GAAACACAAA GAGAGCTCTT ACCTTGAAGG ATATTTTAAA TGGAACATTC 
TCATATAAAA CATATTTTCC CAACTGGATT TCAGAACAAG AATATCTTCA TCAATCTGAG 
GATGATAACA TAGTATTTTA TAATATTGAA ACAAGAGAAT CATATATCAT TTTGAGTAAT 
AGCACCATGA AAAGTGTGAA TGCTACAGAT TATGGTTTGT CACCTGATCG GCAATTTGTG 
TATCTAGAAA GTGATTATTC AAAGCTCTGG CGATATTCAT ACACAGCGAC ATACTACATC 
TACGACCTTC AGAATGGGGA ATTTGTAAGA GGATACGAGC TCCCTCGTCC AATTCAGTAT 
CTATGCTGGT CGCCTGTTGG GAGTAAATTA GCATATGTAT ATCAAAACAA TATTTATTTG 
AAACAAAGAC CAGGAGATCC ACCTTTTCAA ATAACTTATA CTGGAAGAGA AAATAGAATA 
TTTAATGGAA TACCAGACTG GGTTTATGAA GAGGAAATGC TTGCCACAAA ATATGCTCTT 
TGGTGGTCTC CAGATGGAAA ATTTTTGGCA TATGTAGAAT TTAATGATTC AGATATACCA 
ATTATTGCCT ATTCTTATTA TGGTGATGGA CAGTATCCTA GAACTATAAA TATTCCATAT 
CCAAAGGCTG GGGCTAAGAA TCCGGTTGTT CGTGTTTTTA TTGTTGACAC CACCTACCCT 
CACCACGTGG GCCCAATGGA AGTGCCAGTT CCAGAAATGA TAGCCTCAAG TGACTATTAT 
TTCAGCTGGC TCACATGGGT GTCCAGTGAA CGAGTATGCT TGCAGTGGCT AAAAAGAGTG 
CAGAATGTCT CAGTCCTGTC TATATGTGAT TTCAGGGAAG ACTGGCATGC ATGGGAATGT 
CCAAAGAACC AGGAGCATGT AGAAGAAAGC AGAACAGGAT GGGCTGGTGG ATTCTTTGTT 
TCGACACCAG CTTTTAGCCA GGATGCCACT TCTTACTACA AAATATTTAG CGACAAGGAT 
GGTTACAAAC ATATTCACTA CATCAAAGAC ACTGTGGAAA ATGCTATTCA AATTACAAGT 
GGCAAGTGGG AGGCCATATA TATATTCCGC GTAACACAGG ATTCACTGTT TTATTCTAGC 
AATGAATTTG AAGGTTACCC TGGAAGAAGA AACATCTACA GAATTAGCAT TGGAAACTCT 
CCTCCGAGCA AGAAGTGTGT TACTTGCCAT CTAAGGAAAG AAAGGTGCCA ATATTACACA 
GCAAGTTTCA GCTACAAAGC CAAGTACTAT GCACTCGTCT GCTATGGCCC TGGCCTCCCC 
ATTTCCACCC TCCATGATGG CCGCACAGAC CAAGAAATAC AAGTATTAGA AGAAAACAAA 
GAACTGGAAA ATTCTCTGAG AAATATCCAG CTGCCTAAAG TGGAGATTAA GAAGCTCAAA 
GACGGGGGAC TGACTTTCTG GTACAAGATG ATTCTGCCTC CTCAGTTTGA CAGATCAAAG 
AAGTACCCTT TGCTAATTCA AGTGTATGGT GGTCCTTGCA GCCAGAGTGT TAAGTCTGTG 
TTTGCTGTTA ATTGGATAAC TTATCTCGCA AGTAAGGAGG GGATAGTCAT TGCCCTGGTA 
GATGGTCGGG GCACTGCTTT CCAAGGTGAC AAATTCCTGC ATGCCGTGTA TCGAAAACTG 
GGTGTATATG AAGTTGAGGA CCAGCTCACA GCTGTCAGAA AATTCATAGA AATGGGTTTC 
ATTGATGAAG AAAGAATAGC CATATGGGGC TGGTCCTACG GAGGTTATGT TTCATCCCTG 
GCCCTTGCAT CTGGAACTGG TCTTTTCAAA TGTGGCATAG CAGTGGCTCC AGTCTCCAGC 
TGGGAATATT ACGCATCTAT CTACTCAGAG AGATTCATGG GCCTCCCAAC AAAGGACGAC 
AATCTCGAAC ACTATAAAAA TTCAACTGTG ATGGCAAGAG CAGAATATTT CAGAAATGTA 
GACTATCTTC TCATCCACGG AACAGCAGAT GATAATGTGC ACTTTCAGAA CTCAGCACAG 
ATTGCTAAAG CTTTGGTTAA TGCACAAGTG GATTTCCAGG CGATGTGGTA CTCTGACCAG 
AACCATGGTA TATCATCTGG GCGCTCCCAG AATCATTTAT ATACCCACAT GACGCACTTC 
CTCAAGCAAT GCTTTTCTTT ATCAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

FAPalpha, a surface peptidase expressed during wound healing, is a tumor suppressor.
Oncogene23(32)5435-46(2004 Jul)
Ramirez-Montagut T,Blachere NE,Sviderskaya EV,Bennett DC,Rettig WJ,Garin-Chesa P,Houghton AN

Gene-expression profile of collagen-induced arthritis.
Journal of autoimmunity18(2)159-67(2002 Mar)
Ibrahim SM,Koczan D,Thiesen HJ

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Fap cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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