Ddost cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ddost
Entrez Gene ID	13200
Full Name	dolichyl-di-phosphooligosaccharide-protein glycotransferase
General protein information	Preferred Names dolichyl-di-phosphooligosaccharide-protein glycotransferase Names dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit DDOST 48 kDa subunit oligosaccharyl transferase 48 kDa subunit
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_007838.3	NP_031864.2	dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit precursor
NM_007838.2	NP_031864.2	dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DDOST	XP_001161661.1
	LOC718760	XP_001111903.1
Drosophila melanogaster (fruit fly)	Ost48	NP_511096.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006383	XP_316417.4
Homo sapiens (human)	DDOST	NP_005207.2
Xenopus tropicalis (tropical clawed frog)	ddost	NP_001120481.1
Danio rerio (zebrafish)	ddost	NP_998258.1
Mus musculus (house mouse)	Ddost	NP_031864.2
Rattus norvegicus (Norway rat)	Ddost	NP_001012104.1
Oryza sativa (rice)	Os07g0209000	NP_001059165.1
Canis lupus familiaris (dog)	DDOST	NP_001003321.1
Bos taurus (cattle)	DDOST	NP_001094543.1
Gallus gallus (chicken)	DDOST	NP_001161480.1
Caenorhabditis elegans (roundworm)	ostb-1	NP_495655.1
Arabidopsis thaliana (thale cress)	DGL1	NP_569038.1

Gene Ontology
Bibliography

Related articles in PubMed

Increased liver AGEs induce hepatic injury mediated through an OST48 pathway.
Zhuang A, Yap FY, Bruce C, Leung C, Plan MR, Sullivan MA, Herath C, McCarthy D, Sourris KC, Kantharidis P, Coughlan MT, Febbraio MA, Hodson MP, Watt MJ, Angus P, Schulz BL, Forbes JM
Scientific reports7(1)12292(2017 Sep)

Mitotic Phosphorylation of TREX1 C Terminus Disrupts TREX1 Regulation of the Oligosaccharyltransferase Complex.
Kucej M, Fermaintt CS, Yang K, Irizarry-Caro RA, Yan N
Cell reports18(11)2600-2607(2017 Mar)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

Cytosolic Nuclease TREX1 Regulates Oligosaccharyltransferase Activity Independent of Nuclease Activity to Suppress Immune Activation.
Hasan M, Fermaintt CS, Gao N, Sakai T, Miyazaki T, Jiang S, Li QZ, Atkinson JP, Morse HC 3rd, Lehrman MA, Yan N
Immunity43(3)463-74(2015 Sep)

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Koscielny G, Yaikhom G, Iyer V, Meehan TF, Morgan H, Atienza-Herrero J, Blake A, Chen CK, Easty R, Di Fenza A, Fiegel T, Grifiths M, Horne A, Karp NA, Kurbatova N, Mason JC, Matthews P, Oakley DJ, Qazi A, Regnart J, Retha A, Santos LA, Sneddon DJ, Warren J, Westerberg H, Wilson RJ, Melvin DG, Smedley D, Brown SD, Flicek P, Skarnes WC, Mallon AM, Parkinson H
Nucleic acids research42(Database issue)D802-9(2014 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the cell-cycle-dependent post-translation modification of TREX1 regulates its interaction with OST.
Title: Mitotic Phosphorylation of TREX1 C Terminus Disrupts TREX1 Regulation of the Oligosaccharyltransferase Complex.

Data show that oligosaccharyltransferase (OST) activity is dysregulated in three prime exonuclease 1 knockout (Trex1-/-) cells.
Title: Cytosolic Nuclease TREX1 Regulates Oligosaccharyltransferase Activity Independent of Nuclease Activity to Suppress Immune Activation.

AGER1 provides protection against AGE-induced reactive oxygen species generation via NADPH oxidase and protein kinase C delta.
Title: AGER1 regulates endothelial cell NADPH oxidase-dependent oxidant stress via PKC-delta: implications for vascular disease.

The following Ddost gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ddost cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu00894	NM_007838.3 Latest version!	Mus musculus dolichyl-di-phosphooligosaccharide-protein glycotransferase (Ddost), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$258.30 $369.00
View Old Accession Versions >>
OMu00894	NM_007838.2	Mus musculus dolichyl-di-phosphooligosaccharide-protein glycotransferase (Ddost), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$258.30 $369.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ddost ( NM_007838.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031864.2 Mus musculus dolichyl-di-phosphooligosaccharide-protein glycotransferase (Ddost), mRNA.

Price & Availability↑

CloneID	OMu00894
Clone ID Related Accession (Same CDS sequence)	NM_007838.3 , NM_007838.2
Accession Version	NM_007838.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-22
Organism	Mus musculus(house mouse)
Product	dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC068132.1 and BU755176.1. On Apr 27, 2018 this sequence version replaced NM_007838.2. ##Evidence-Data-START## Transcript exon combination :: BC068132.1, AK084267.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGATGG ATCCCCGCCT CGCCGTCCGC GCCTGGCCCC TCTGCGGGCT GCTGCTGGCC 
GTGCTCGGCT GCGTCTGCGC CAGCGGCCCC CGCACCCTCG TGCTGCTGGA CAACCTGAAC 
GTGCGGGACA CGCACTCGCT GTTCTTCCGC AGCCTGAAGG ACCGGGGCTT TGAGCTCACC 
TTCAAGACCG CAGATGACCC CAGCTTGTCC CTCATTAAGT ACGGGGAGTT CCTCTATGAC 
AACCTTATCA TCTTTTCCCC GTCGGTGGAA GATTTTGGAG GCAACATCAA CGTGGAGACC 
ATCAGTGCCT TCATTGATGG TGGCGGCAGC GTTTTGGTGG CTGCCAGCTC TGATATTGGT 
GACCCTCTTC GGGAGCTGGG CAGTGAGTGT GGGATTGAAT TTGATGAAGA GAAAACAGCT 
GTCATCGACC ACCACAACTA TGATGTTTCT GACCTTGGCC AGCACACACT CATTGTGGCT 
GACACTGAGA ACCTGCTGAA GGCCCCGACC ATTGTTGGCA AGTCATCTCT GAACCCCATT 
CTCTTCCGAG GAGTTGGAAT GGTGGCAGAC CCGGACAATC CCTTGGTTTT GGACATCCTA 
ACAGGCTCTT CAACCTCTTA CTCCTTCTTC CCAGATAAAC CAATCACCCA GTACCCCCAT 
GCGGTGGGGA GGAACACTCT GCTGATTGCC GGGCTCCAGG CCAGGAACAA CGCCCGGGTC 
ATCTTCAGTG GCTCTCTGGA TTTCTTCAGC GATGCCTTCT TCAACTCGGC AGTGCAGAAG 
GCCACACCCG GTGCGCAGAG GTATTCTCAG ACAGGCAACT ATGAACTAGC TGTGGCCCTC 
TCACGCTGGG TGTTCAAGGA GGAGGGTGTC CTTCGAGTAG GGCCTGTGTC CCATCACCGG 
GTGGGCGAGA TGGCTCCACC CAATGCCTAC ACTGTCACCG ACTTGGTGGA GTATAGCATC 
ATCATAGAAC AGCTCTCCAA TGGCAAGTGG GTCCCCTTTG ATGGTGATGA CATTCAGCTG 
GAGTTCGTGC GCATCGACCC CTTCGTGAGG ACCTTCCTGA AGAGGAAAGG TGGCAAGTAC 
AGTGTCCAGT TCAAGCTGCC TGACGTGTAT GGTGTATTCC AGTTTAAAGT GGATTACAAC 
CGGCTAGGCT ACACCCACCT GTACTCTTCC ACCCAGGTGT CAGTGAGGCC ACTCCAGCAC 
ACGCAGTATG AGCGCTTCAT CCCCTCGGCC TATCCCTACT ATGCCAGTGC CTTCTCCATG 
ATGGCCGGGC TCTTCATCTT CAGCATCGTC TTCTTGCACA TGAAGGAGAA GGAGAAGTCT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031864.2
CDS	142..1467
Translation	MKMDPRLAVRAWPLCGLLLAVLGCVCASGPRTLVLLDNLNVRDTHSLFFRSLKDRGFELTFKTADDPSLSLIKYGEFLYDNLIIFSPSVEDFGGNINVETISAFIDGGGSVLVAASSDIGDPLRELGSECGIEFDEEKTAVIDHHNYDVSDLGQHTLIVADTENLLKAPTIVGKSSLNPILFRGVGMVADPDNPLVLDILTGSSTSYSFFPDKPITQYPHAVGRNTLLIAGLQARNNARVIFSGSLDFFSDAFFNSAVQKATPGAQRYSQTGNYELAVALSRWVFKEEGVLRVGPVSHHRVGEMAPPNAYTVTDLVEYSIIIEQLSNGKWVPFDGDDIQLEFVRIDPFVRTFLKRKGGKYSVQFKLPDVYGVFQFKVDYNRLGYTHLYSSTQVSVRPLQHTQYERFIPSAYPYYASAFSMMAGLFIFSIVFLHMKEKEKSD

Target ORF information:

RefSeq Version	NM_007838.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus dolichyl-di-phosphooligosaccharide-protein glycotransferase (Ddost), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007838.3

ORF Insert Sequence:

ATGAAGATGG ATCCCCGCCT CGCCGTCCGC GCCTGGCCCC TCTGCGGGCT GCTGCTGGCC 
GTGCTCGGCT GCGTCTGCGC CAGCGGCCCC CGCACCCTCG TGCTGCTGGA CAACCTGAAC 
GTGCGGGACA CGCACTCGCT GTTCTTCCGC AGCCTGAAGG ACCGGGGCTT TGAGCTCACC 
TTCAAGACCG CAGATGACCC CAGCTTGTCC CTCATTAAGT ACGGGGAGTT CCTCTATGAC 
AACCTTATCA TCTTTTCCCC GTCGGTGGAA GATTTTGGAG GCAACATCAA CGTGGAGACC 
ATCAGTGCCT TCATTGATGG TGGCGGCAGC GTTTTGGTGG CTGCCAGCTC TGATATTGGT 
GACCCTCTTC GGGAGCTGGG CAGTGAGTGT GGGATTGAAT TTGATGAAGA GAAAACAGCT 
GTCATCGACC ACCACAACTA TGATGTTTCT GACCTTGGCC AGCACACACT CATTGTGGCT 
GACACTGAGA ACCTGCTGAA GGCCCCGACC ATTGTTGGCA AGTCATCTCT GAACCCCATT 
CTCTTCCGAG GAGTTGGAAT GGTGGCAGAC CCGGACAATC CCTTGGTTTT GGACATCCTA 
ACAGGCTCTT CAACCTCTTA CTCCTTCTTC CCAGATAAAC CAATCACCCA GTACCCCCAT 
GCGGTGGGGA GGAACACTCT GCTGATTGCC GGGCTCCAGG CCAGGAACAA CGCCCGGGTC 
ATCTTCAGTG GCTCTCTGGA TTTCTTCAGC GATGCCTTCT TCAACTCGGC AGTGCAGAAG 
GCCACACCCG GTGCGCAGAG GTATTCTCAG ACAGGCAACT ATGAACTAGC TGTGGCCCTC 
TCACGCTGGG TGTTCAAGGA GGAGGGTGTC CTTCGAGTAG GGCCTGTGTC CCATCACCGG 
GTGGGCGAGA TGGCTCCACC CAATGCCTAC ACTGTCACCG ACTTGGTGGA GTATAGCATC 
ATCATAGAAC AGCTCTCCAA TGGCAAGTGG GTCCCCTTTG ATGGTGATGA CATTCAGCTG 
GAGTTCGTGC GCATCGACCC CTTCGTGAGG ACCTTCCTGA AGAGGAAAGG TGGCAAGTAC 
AGTGTCCAGT TCAAGCTGCC TGACGTGTAT GGTGTATTCC AGTTTAAAGT GGATTACAAC 
CGGCTAGGCT ACACCCACCT GTACTCTTCC ACCCAGGTGT CAGTGAGGCC ACTCCAGCAC 
ACGCAGTATG AGCGCTTCAT CCCCTCGGCC TATCCCTACT ATGCCAGTGC CTTCTCCATG 
ATGGCCGGGC TCTTCATCTT CAGCATCGTC TTCTTGCACA TGAAGGAGAA GGAGAAGTCT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ddost ( NM_007838.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031864.2 Mus musculus dolichyl-di-phosphooligosaccharide-protein glycotransferase (Ddost), mRNA.

Price & Availability↑

CloneID	OMu00894
Clone ID Related Accession (Same CDS sequence)	NM_007838.3 , NM_007838.2
Accession Version	NM_007838.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Mus musculus(house mouse)
Product	dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC068132.1. On Apr 5, 2004 this sequence version replaced NM_007838.1. ##Evidence-Data-START## Transcript exon combination :: BC068132.1, AK084267.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END##

ATGAAGATGG ATCCCCGCCT CGCCGTCCGC GCCTGGCCCC TCTGCGGGCT GCTGCTGGCC 
GTGCTCGGCT GCGTCTGCGC CAGCGGCCCC CGCACCCTCG TGCTGCTGGA CAACCTGAAC 
GTGCGGGACA CGCACTCGCT GTTCTTCCGC AGCCTGAAGG ACCGGGGCTT TGAGCTCACC 
TTCAAGACCG CAGATGACCC CAGCTTGTCC CTCATTAAGT ACGGGGAGTT CCTCTATGAC 
AACCTTATCA TCTTTTCCCC GTCGGTGGAA GATTTTGGAG GCAACATCAA CGTGGAGACC 
ATCAGTGCCT TCATTGATGG TGGCGGCAGC GTTTTGGTGG CTGCCAGCTC TGATATTGGT 
GACCCTCTTC GGGAGCTGGG CAGTGAGTGT GGGATTGAAT TTGATGAAGA GAAAACAGCT 
GTCATCGACC ACCACAACTA TGATGTTTCT GACCTTGGCC AGCACACACT CATTGTGGCT 
GACACTGAGA ACCTGCTGAA GGCCCCGACC ATTGTTGGCA AGTCATCTCT GAACCCCATT 
CTCTTCCGAG GAGTTGGAAT GGTGGCAGAC CCGGACAATC CCTTGGTTTT GGACATCCTA 
ACAGGCTCTT CAACCTCTTA CTCCTTCTTC CCAGATAAAC CAATCACCCA GTACCCCCAT 
GCGGTGGGGA GGAACACTCT GCTGATTGCC GGGCTCCAGG CCAGGAACAA CGCCCGGGTC 
ATCTTCAGTG GCTCTCTGGA TTTCTTCAGC GATGCCTTCT TCAACTCGGC AGTGCAGAAG 
GCCACACCCG GTGCGCAGAG GTATTCTCAG ACAGGCAACT ATGAACTAGC TGTGGCCCTC 
TCACGCTGGG TGTTCAAGGA GGAGGGTGTC CTTCGAGTAG GGCCTGTGTC CCATCACCGG 
GTGGGCGAGA TGGCTCCACC CAATGCCTAC ACTGTCACCG ACTTGGTGGA GTATAGCATC 
ATCATAGAAC AGCTCTCCAA TGGCAAGTGG GTCCCCTTTG ATGGTGATGA CATTCAGCTG 
GAGTTCGTGC GCATCGACCC CTTCGTGAGG ACCTTCCTGA AGAGGAAAGG TGGCAAGTAC 
AGTGTCCAGT TCAAGCTGCC TGACGTGTAT GGTGTATTCC AGTTTAAAGT GGATTACAAC 
CGGCTAGGCT ACACCCACCT GTACTCTTCC ACCCAGGTGT CAGTGAGGCC ACTCCAGCAC 
ACGCAGTATG AGCGCTTCAT CCCCTCGGCC TATCCCTACT ATGCCAGTGC CTTCTCCATG 
ATGGCCGGGC TCTTCATCTT CAGCATCGTC TTCTTGCACA TGAAGGAGAA GGAGAAGTCT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031864.2
CDS	142..1467
Translation	MKMDPRLAVRAWPLCGLLLAVLGCVCASGPRTLVLLDNLNVRDTHSLFFRSLKDRGFELTFKTADDPSLSLIKYGEFLYDNLIIFSPSVEDFGGNINVETISAFIDGGGSVLVAASSDIGDPLRELGSECGIEFDEEKTAVIDHHNYDVSDLGQHTLIVADTENLLKAPTIVGKSSLNPILFRGVGMVADPDNPLVLDILTGSSTSYSFFPDKPITQYPHAVGRNTLLIAGLQARNNARVIFSGSLDFFSDAFFNSAVQKATPGAQRYSQTGNYELAVALSRWVFKEEGVLRVGPVSHHRVGEMAPPNAYTVTDLVEYSIIIEQLSNGKWVPFDGDDIQLEFVRIDPFVRTFLKRKGGKYSVQFKLPDVYGVFQFKVDYNRLGYTHLYSSTQVSVRPLQHTQYERFIPSAYPYYASAFSMMAGLFIFSIVFLHMKEKEKSD

Target ORF information:

RefSeq Version	NM_007838.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus dolichyl-di-phosphooligosaccharide-protein glycotransferase (Ddost), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007838.2

ORF Insert Sequence:

ATGAAGATGG ATCCCCGCCT CGCCGTCCGC GCCTGGCCCC TCTGCGGGCT GCTGCTGGCC 
GTGCTCGGCT GCGTCTGCGC CAGCGGCCCC CGCACCCTCG TGCTGCTGGA CAACCTGAAC 
GTGCGGGACA CGCACTCGCT GTTCTTCCGC AGCCTGAAGG ACCGGGGCTT TGAGCTCACC 
TTCAAGACCG CAGATGACCC CAGCTTGTCC CTCATTAAGT ACGGGGAGTT CCTCTATGAC 
AACCTTATCA TCTTTTCCCC GTCGGTGGAA GATTTTGGAG GCAACATCAA CGTGGAGACC 
ATCAGTGCCT TCATTGATGG TGGCGGCAGC GTTTTGGTGG CTGCCAGCTC TGATATTGGT 
GACCCTCTTC GGGAGCTGGG CAGTGAGTGT GGGATTGAAT TTGATGAAGA GAAAACAGCT 
GTCATCGACC ACCACAACTA TGATGTTTCT GACCTTGGCC AGCACACACT CATTGTGGCT 
GACACTGAGA ACCTGCTGAA GGCCCCGACC ATTGTTGGCA AGTCATCTCT GAACCCCATT 
CTCTTCCGAG GAGTTGGAAT GGTGGCAGAC CCGGACAATC CCTTGGTTTT GGACATCCTA 
ACAGGCTCTT CAACCTCTTA CTCCTTCTTC CCAGATAAAC CAATCACCCA GTACCCCCAT 
GCGGTGGGGA GGAACACTCT GCTGATTGCC GGGCTCCAGG CCAGGAACAA CGCCCGGGTC 
ATCTTCAGTG GCTCTCTGGA TTTCTTCAGC GATGCCTTCT TCAACTCGGC AGTGCAGAAG 
GCCACACCCG GTGCGCAGAG GTATTCTCAG ACAGGCAACT ATGAACTAGC TGTGGCCCTC 
TCACGCTGGG TGTTCAAGGA GGAGGGTGTC CTTCGAGTAG GGCCTGTGTC CCATCACCGG 
GTGGGCGAGA TGGCTCCACC CAATGCCTAC ACTGTCACCG ACTTGGTGGA GTATAGCATC 
ATCATAGAAC AGCTCTCCAA TGGCAAGTGG GTCCCCTTTG ATGGTGATGA CATTCAGCTG 
GAGTTCGTGC GCATCGACCC CTTCGTGAGG ACCTTCCTGA AGAGGAAAGG TGGCAAGTAC 
AGTGTCCAGT TCAAGCTGCC TGACGTGTAT GGTGTATTCC AGTTTAAAGT GGATTACAAC 
CGGCTAGGCT ACACCCACCT GTACTCTTCC ACCCAGGTGT CAGTGAGGCC ACTCCAGCAC 
ACGCAGTATG AGCGCTTCAT CCCCTCGGCC TATCCCTACT ATGCCAGTGC CTTCTCCATG 
ATGGCCGGGC TCTTCATCTT CAGCATCGTC TTCTTGCACA TGAAGGAGAA GGAGAAGTCT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Increased liver AGEs induce hepatic injury mediated through an OST48 pathway.
Scientific reports7(1)12292(2017 Sep)
Zhuang A,Yap FY,Bruce C,Leung C,Plan MR,Sullivan MA,Herath C,McCarthy D,Sourris KC,Kantharidis P,Coughlan MT,Febbraio MA,Hodson MP,Watt MJ,Angus P,Schulz BL,Forbes JM

Mitotic Phosphorylation of TREX1 C Terminus Disrupts TREX1 Regulation of the Oligosaccharyltransferase Complex.
Cell reports18(11)2600-2607(2017 Mar)
Kucej M,Fermaintt CS,Yang K,Irizarry-Caro RA,Yan N

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

Cytosolic Nuclease TREX1 Regulates Oligosaccharyltransferase Activity Independent of Nuclease Activity to Suppress Immune Activation.
Immunity43(3)463-74(2015 Sep)
Hasan M,Fermaintt CS,Gao N,Sakai T,Miyazaki T,Jiang S,Li QZ,Atkinson JP,Morse HC 3rd,Lehrman MA,Yan N

The International Mouse Phenotyping Consortium Web Portal, a unified point of access for knockout mice and related phenotyping data.
Nucleic acids research42(Database issue)D802-9(2014 Jan)
Koscielny G,Yaikhom G,Iyer V,Meehan TF,Morgan H,Atienza-Herrero J,Blake A,Chen CK,Easty R,Di Fenza A,Fiegel T,Grifiths M,Horne A,Karp NA,Kurbatova N,Mason JC,Matthews P,Oakley DJ,Qazi A,Regnart J,Retha A,Santos LA,Sneddon DJ,Warren J,Westerberg H,Wilson RJ,Melvin DG,Smedley D,Brown SD,Flicek P,Skarnes WC,Mallon AM,Parkinson H

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Ddost cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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