Cort cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cort
Entrez Gene ID	12854
Full Name	cortistatin
Synonyms	CST,PCST
General protein information	Preferred Names cortistatin Names cortistatin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4 E2\|4 78.89 cM
Summary	This gene encodes a member of the somatostatin family of multifunctional peptides attributed with neurohormone, neurotransmitter/modulator and autocrine/paracrine actions. The encoded preproprotein undergoes proteolytic processing to generate a mature functional peptide that can bind to somatostatin receptors. Mice lacking the encoded protein exhibit elevated levels of growth hormone in the plasma without major changes in somatic growth and have exacerbated nociceptive responses to neuropathic and inflammatory pain sensitization. Transgenic mice overexpressing the encoded protein in neurons do not express long-term potentiation in the dentate gyrus and exhibit deficits in synaptic plasticity and learning. [provided by RefSeq, Nov 2015].

mRNA and Protein(s)

mRNA	Protein	Name
XM_030253155.1	XP_030109015.1	cortistatin isoform X1
NM_007745.4	NP_031771.1	cortistatin preproprotein

Pathways from BioSystems

Homologies

Homo sapiens (human)	CORT	NP_001293.3
Pan troglodytes (chimpanzee)	CORT	NP_001191822.1
Bos taurus (cattle)	CORT	NP_001191825.1
Mus musculus (house mouse)	Cort	NP_031771.1
Rattus norvegicus (Norway rat)	Cort	NP_036967.1

Gene Ontology
Bibliography

Related articles in PubMed

Diverse Central Projection Patterns of Retinal Ganglion Cells.
Martersteck EM, Hirokawa KE, Evarts M, Bernard A, Duan X, Li Y, Ng L, Oh SW, Ouellette B, Royall JJ, Stoecklin M, Wang Q, Zeng H, Sanes JR, Harris JA
Cell reports18(8)2058-2072(2017 Feb)

Obesity- and gender-dependent role of endogenous somatostatin and cortistatin in the regulation of endocrine and metabolic homeostasis in mice.
Luque RM, Cordoba-Chacon J, Pozo-Salas AI, Porteiro B, de Lecea L, Nogueiras R, Gahete MD, Casta?o JP
Scientific reports637992(2016 Nov)

Fasting modulates GH/IGF-I axis and its regulatory systems in the mammary gland of female mice: Influence of endogenous cortistatin.
Villa-Osaba A, Gahete MD, Cordoba-Chacon J, de Lecea L, Casta?o JP, Luque RM
Molecular and cellular endocrinology43414-24(2016 Oct)

Cortistatin Is a Key Factor Regulating the Sex-Dependent Response of the GH and Stress Axes to Fasting in Mice.
Cordoba-Chac?n J, Gahete MD, Pozo-Salas AI, de Lecea L, Casta?o JP, Luque RM
Endocrinology157(7)2810-23(2016 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Endogenous SST and CORT distinctly impact on DMBA-induced mammary gland tumorigenesis , in a manner that is strongly dependent on the metabolic/endocrine milieu (lean vs. obese status).
Title: Lack of cortistatin or somatostatin differentially influences DMBA-induced mammary gland tumorigenesis in mice in an obesity-dependent mode.

This study demonistrated that Cortistatin attenuates inflammatory pain via spinal and peripheral actions.
Title: Cortistatin attenuates inflammatory pain via spinal and peripheral actions.

Cortistatin emerges as a natural endogenous regulator of smooth muscle cells under pathological conditions and an attractive candidate for the pharmacological management of vascular diseases that course with neointimal lesion formation.
Title: Cortistatin inhibits migration and proliferation of human vascular smooth muscle cells and decreases neointimal formation on carotid artery ligation.

endogenous CORT is essential for the obesity-induced changes in insulin expression/secretion observed in mice
Title: Role of endogenous cortistatin in the regulation of ghrelin system expression at pancreatic level under normal and obese conditions.

the role of endogenous CST is different from SST; CST exerted an unexpected stimulatory role on prolactin secretion
Title: Cortistatin is not a somatostatin analogue but stimulates prolactin release and inhibits GH and ACTH in a gender-dependent fashion: potential role of ghrelin.

The following Cort gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cort cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu14812	NM_007745.4 Latest version!	Mus musculus cortistatin (Cort), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu88406	XM_030253155.1 Latest version!	Mus musculus cortistatin (Cort), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cort ( NM_007745.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031771.1 Mus musculus cortistatin (Cort), mRNA.

Price & Availability↑

CloneID	OMu14812
Clone ID Related Accession (Same CDS sequence)	NM_007745.4
Accession Version	NM_007745.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Mus musculus(house mouse)
Product	cortistatin preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL731655.8, AK013496.1 and AW122642.1. On Nov 25, 2015 this sequence version replaced NM_007745.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AW490859.1, AK013496.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849383, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGGTG GCCGAGGCAC AGGAGGCAAG TGGCCCTCAG CCTTCGGGCT GCTGCTGCTC 
TGGGGGGTCG CAGCCTCCGC CCTTCCCCTG GAGAGTGGCC CTACTGGCCA GGACAGTGTG 
CAGGAAGCCA CCGAGGGGAG GAGCGGCCTT CTGACTTTCC TTGCCTGGTG GCACGAGTGG 
GCTTCCCAAG CCAGCTCCAG CACCCCCGTC GGAGGGGGTA CCCCCGGGCT GTCCAAGAGC 
CAGGAAAGGC CACCCCCCCA ACAGCCCCCA CACCTGGATA AAAAGCCCTG CAAGAACTTC 
TTCTGGAAAA CCTTCTCCTC GTGCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031771.1
CDS	118..447
Translation	MMGGRGTGGKWPSAFGLLLLWGVAASALPLESGPTGQDSVQEATEGRSGLLTFLAWWHEWASQASSSTPVGGGTPGLSKSQERPPPQQPPHLDKKPCKNFFWKTFSSCK

Target ORF information:

RefSeq Version	NM_007745.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus cortistatin (Cort), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007745.4

ORF Insert Sequence:

ATGATGGGTG GCCGAGGCAC AGGAGGCAAG TGGCCCTCAG CCTTCGGGCT GCTGCTGCTC 
TGGGGGGTCG CAGCCTCCGC CCTTCCCCTG GAGAGTGGCC CTACTGGCCA GGACAGTGTG 
CAGGAAGCCA CCGAGGGGAG GAGCGGCCTT CTGACTTTCC TTGCCTGGTG GCACGAGTGG 
GCTTCCCAAG CCAGCTCCAG CACCCCCGTC GGAGGGGGTA CCCCCGGGCT GTCCAAGAGC 
CAGGAAAGGC CACCCCCCCA ACAGCCCCCA CACCTGGATA AAAAGCCCTG CAAGAACTTC 
TTCTGGAAAA CCTTCTCCTC GTGCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cort ( XM_030253155.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109015.1 Mus musculus cortistatin (Cort), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu88406
Clone ID Related Accession (Same CDS sequence)	XM_030253155.1
Accession Version	XM_030253155.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	cortistatin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGTG GCCGAGGCAC AGGAGGCAAG TGGCCCTCAG CCTTCGGGCT GCTGCTGCTC 
TGGGGGGTCG CAGCCTCCGC CCTTCCCCTG GAGAGTGGCC CTACTGGCCA GGACAGTGTG 
GAAGCCACCG AGGGGAGGAG CGGCCTTCTG ACTTTCCTTG CCTGGTGGCA CGAGTGGGCT 
TCCCAAGCCA GCTCCAGCAC CCCCGTCGGA GGGGGTACCC CCGGGCTGTC CAAGAGCCAG 
GAAAGGCCAC CCCCCCAACA GCCCCCACAC CTGGATAAAA AGCCCTGCAA GAACTTCTTC 
TGGAAAACCT TCTCCTCGTG CAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109015.1
CDS	32..358
Translation	MMGGRGTGGKWPSAFGLLLLWGVAASALPLESGPTGQDSVEATEGRSGLLTFLAWWHEWASQASSSTPVGGGTPGLSKSQERPPPQQPPHLDKKPCKNFFWKTFSSCK

Target ORF information:

RefSeq Version	XM_030253155.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus cortistatin (Cort), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253155.1

ORF Insert Sequence:

ATGATGGGTG GCCGAGGCAC AGGAGGCAAG TGGCCCTCAG CCTTCGGGCT GCTGCTGCTC 
TGGGGGGTCG CAGCCTCCGC CCTTCCCCTG GAGAGTGGCC CTACTGGCCA GGACAGTGTG 
GAAGCCACCG AGGGGAGGAG CGGCCTTCTG ACTTTCCTTG CCTGGTGGCA CGAGTGGGCT 
TCCCAAGCCA GCTCCAGCAC CCCCGTCGGA GGGGGTACCC CCGGGCTGTC CAAGAGCCAG 
GAAAGGCCAC CCCCCCAACA GCCCCCACAC CTGGATAAAA AGCCCTGCAA GAACTTCTTC 
TGGAAAACCT TCTCCTCGTG CAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Diverse Central Projection Patterns of Retinal Ganglion Cells.
Cell reports18(8)2058-2072(2017 Feb)
Martersteck EM,Hirokawa KE,Evarts M,Bernard A,Duan X,Li Y,Ng L,Oh SW,Ouellette B,Royall JJ,Stoecklin M,Wang Q,Zeng H,Sanes JR,Harris JA

Obesity- and gender-dependent role of endogenous somatostatin and cortistatin in the regulation of endocrine and metabolic homeostasis in mice.
Scientific reports637992(2016 Nov)
Luque RM,Cordoba-Chacon J,Pozo-Salas AI,Porteiro B,de Lecea L,Nogueiras R,Gahete MD,Casta?o JP

Fasting modulates GH/IGF-I axis and its regulatory systems in the mammary gland of female mice: Influence of endogenous cortistatin.
Molecular and cellular endocrinology43414-24(2016 Oct)
Villa-Osaba A,Gahete MD,Cordoba-Chacon J,de Lecea L,Casta?o JP,Luque RM

Cortistatin Is a Key Factor Regulating the Sex-Dependent Response of the GH and Stress Axes to Fasting in Mice.
Endocrinology157(7)2810-23(2016 Jul)
Cordoba-Chac?n J,Gahete MD,Pozo-Salas AI,de Lecea L,Casta?o JP,Luque RM

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Cort cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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