Ckb cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ckb
Entrez Gene ID	12709
Full Name	creatine kinase, brain
Synonyms	B-CK,Bck,Ck-3,Ck3,Ckbb
General protein information	Preferred Names creatine kinase, brain Names creatine kinase B-type creatine kinase B chain
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 12 Map Location: 12 F1\|12 61.09 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_021273.4	NP_067248.1	creatine kinase B-type

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	ckba	NP_001070631.1
Mus musculus (house mouse)	Ckb	NP_067248.1
Danio rerio (zebrafish)	ckbb	NP_775329.1
Homo sapiens (human)	CKB	NP_001814.2
Bos taurus (cattle)	CKB	NP_001015613.1
	CKB	XP_001112108.1
Rattus norvegicus (Norway rat)	Ckb	NP_036661.3
Gallus gallus (chicken)	CKB	NP_990641.1
Xenopus tropicalis (tropical clawed frog)	ckb	NP_001037926.1
Pan troglodytes (chimpanzee)	CKB	XP_510185.3
Canis lupus familiaris (dog)	CKB	XP_003639259.1

Gene Ontology
Bibliography

Related articles in PubMed

Cell-Type-Specific Spatiotemporal Expression of Creatine Biosynthetic Enzyme S-adenosylmethionine:guanidinoacetate N-methyltransferase in Developing Mouse Brain.
Tachikawa M, Watanabe M, Fukaya M, Sakai K, Terasaki T, Hosoya KI
Neurochemical research(2017 Dec)

Creatine kinase B is necessary to limit myoblast fusion during myogenesis.
Simionescu-Bankston A, Pichavant C, Canner JP, Apponi LH, Wang Y, Steeds C, Olthoff JT, Belanto JJ, Ervasti JM, Pavlath GK
American journal of physiology. Cell physiology308(11)C919-31(2015 Jun)

Analysis of Presenilin 1 and 2 interacting proteins in mouse cerebral cortex during development.
Kumar A, Thakur MK
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience38138-46(2014 Nov)

Isoaspartyl formation in creatine kinase B is associated with loss of enzymatic activity; implications for the linkage of isoaspartate accumulation and neurological dysfunction in the PIMT knockout mouse.
Dimitrijevic A, Qin Z, Aswad DW
PloS one9(6)e100622(2014)

Genetic control of autoimmune myocarditis mediated by myosin-specific antibodies.
Kuan AP, Chamberlain W, Malkiel S, Lieu HD, Factor SM, Diamond B, Kotzin BL
Immunogenetics49(2)79-85(1999 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CKB plays a key role in myotube formation by limiting myoblast fusion during myogenesis.
Title: Creatine kinase B is necessary to limit myoblast fusion during myogenesis.

BCK phosphorylation at Ser6 did not affect its enzymatic activity, but led to the appearance of the phosphorylated enzyme at the endoplasmic reticulum (ER)
Title: Regulation of brain-type creatine kinase by AMP-activated protein kinase: interaction, phosphorylation and ER localization.

Loss of normal CKB structure and function contributes to the mechanisms by which isoaspartate accumulation leads to central nervous system dysfunction in the PIMT-Knockout mouse.
Title: Isoaspartyl formation in creatine kinase B is associated with loss of enzymatic activity; implications for the linkage of isoaspartate accumulation and neurological dysfunction in the PIMT knockout mouse.

Hypothalamic plasticity of neuropeptide Y is lacking in brain-type creatine kinase double knockout mice with defective thermoregulation
Title: Hypothalamic plasticity of neuropeptide Y is lacking in brain-type creatine kinase double knockout mice with defective thermoregulation.

negative regulation of CKB by mHTT is a key event in the pathogenesis of HD and contributes to the neuronal dysfunction associated with HD.
Title: Enhancement of brain-type creatine kinase activity ameliorates neuronal deficits in Huntington's disease.

The following Ckb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ckb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu00653	NM_021273.4 Latest version!	Mus musculus creatine kinase, brain (Ckb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ckb ( NM_021273.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067248.1 Mus musculus creatine kinase, brain (Ckb), mRNA.

Price & Availability↑

CloneID	OMu00653
Clone ID Related Accession (Same CDS sequence)	NM_021273.4
Accession Version	NM_021273.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1146bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Mus musculus(house mouse)
Product	creatine kinase B-type
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC160972.4, AK167034.1 and BY654507.1. On Aug 28, 2009 this sequence version replaced NM_021273.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK002467.1, AK166980.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTTCT CCAACAGCCA TAATACGCAG AAGCTGCGCT TCCCGGCCGA GGATGAGTTC 
CCTGATCTGA GCAGCCACAA CAACCATATG GCCAAGGTGC TGACCCCGGA GCTGTACGCC 
GAGCTCCGTG CCAAGTGCAC GCCGAGCGGC TTTACTTTGG ACGACGCCAT TCAGACTGGC 
GTAGACAATC CGGGCCACCC GTACATCATG ACTGTGGGTG CAGTGGCGGG CGACGAGGAG 
AGTTACGACG TATTCAAGGA CCTCTTCGAC CCCATTATTG AGGAGCGGCA CGGCGGCTAC 
CAGCCCAGTG ATGAGCACAA GACCGACCTC AACCCAGACA ACCTGCAGGG TGGCGATGAC 
CTGGACCCCA ACTACGTGCT GAGCTCGCGA GTGCGCACAG GCCGCAGCAT CCGCGGCTTC 
TGTCTCCCCC CGCACTGCAG CCGCGGGGAG CGCCGCGCCA TCGAGAAGCT GGCAGTAGAA 
GCTCTGTCCA GCCTAGATGG CGACCTGTCT GGCAGGTACT ACGCGCTCAA GAGCATGACT 
GAGGCGGAGC AGCAGCAGCT CATTGACGAC CACTTCCTCT TCGATAAGCC TGTGTCGCCT 
CTGCTGCTGG CCTCCGGCAT GGCCCGGGAC TGGCCGGATG CTCGTGGCAT ATGGCACAAT 
GACAATAAGA CTTTCCTGGT GTGGATTAAC GAGGAGGACC ACCTGCGAGT CATCTCCATG 
CAGAAGGGGG GCAACATGAA GGAAGTGTTC ACCCGATTCT GCACCGGCCT CACTCAGATC 
GAAACTCTCT TCAAGTCCAA GAACTATGAG TTCATGTGGA ATCCTCACCT GGGCTACATC 
CTCACATGCC CATCCAACCT GGGCACCGGA CTGCGGGCAG GTGTGCACAT CAAGCTGCCC 
CACCTGGGGA AGCACGAGAA GTTCTCGGAG GTGCTCAAGC GGCTGCGGCT TCAGAAGCGA 
GGCACAGGTG GCGTGGACAC CGCTGCTGTC GGTGGGGTGT TTGATGTCTC CAACGCTGAC 
CGCCTGGGCT TCTCGGAGGT GGAACTGGTG CAGATGGTGG TGGACGGAGT GAAACTACTC 
ATTGAGATGG AGCAACGGCT GGAGCAGGGT CAGGCAATCG ATGACCTCAT GCCGGCCCAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067248.1
CDS	125..1270
Translation	MPFSNSHNTQKLRFPAEDEFPDLSSHNNHMAKVLTPELYAELRAKCTPSGFTLDDAIQTGVDNPGHPYIMTVGAVAGDEESYDVFKDLFDPIIEERHGGYQPSDEHKTDLNPDNLQGGDDLDPNYVLSSRVRTGRSIRGFCLPPHCSRGERRAIEKLAVEALSSLDGDLSGRYYALKSMTEAEQQQLIDDHFLFDKPVSPLLLASGMARDWPDARGIWHNDNKTFLVWINEEDHLRVISMQKGGNMKEVFTRFCTGLTQIETLFKSKNYEFMWNPHLGYILTCPSNLGTGLRAGVHIKLPHLGKHEKFSEVLKRLRLQKRGTGGVDTAAVGGVFDVSNADRLGFSEVELVQMVVDGVKLLIEMEQRLEQGQAIDDLMPAQK

Target ORF information:

RefSeq Version	NM_021273.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus creatine kinase, brain (Ckb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021273.4

ORF Insert Sequence:

ATGCCCTTCT CCAACAGCCA TAATACGCAG AAGCTGCGCT TCCCGGCCGA GGATGAGTTC 
CCTGATCTGA GCAGCCACAA CAACCATATG GCCAAGGTGC TGACCCCGGA GCTGTACGCC 
GAGCTCCGTG CCAAGTGCAC GCCGAGCGGC TTTACTTTGG ACGACGCCAT TCAGACTGGC 
GTAGACAATC CGGGCCACCC GTACATCATG ACTGTGGGTG CAGTGGCGGG CGACGAGGAG 
AGTTACGACG TATTCAAGGA CCTCTTCGAC CCCATTATTG AGGAGCGGCA CGGCGGCTAC 
CAGCCCAGTG ATGAGCACAA GACCGACCTC AACCCAGACA ACCTGCAGGG TGGCGATGAC 
CTGGACCCCA ACTACGTGCT GAGCTCGCGA GTGCGCACAG GCCGCAGCAT CCGCGGCTTC 
TGTCTCCCCC CGCACTGCAG CCGCGGGGAG CGCCGCGCCA TCGAGAAGCT GGCAGTAGAA 
GCTCTGTCCA GCCTAGATGG CGACCTGTCT GGCAGGTACT ACGCGCTCAA GAGCATGACT 
GAGGCGGAGC AGCAGCAGCT CATTGACGAC CACTTCCTCT TCGATAAGCC TGTGTCGCCT 
CTGCTGCTGG CCTCCGGCAT GGCCCGGGAC TGGCCGGATG CTCGTGGCAT ATGGCACAAT 
GACAATAAGA CTTTCCTGGT GTGGATTAAC GAGGAGGACC ACCTGCGAGT CATCTCCATG 
CAGAAGGGGG GCAACATGAA GGAAGTGTTC ACCCGATTCT GCACCGGCCT CACTCAGATC 
GAAACTCTCT TCAAGTCCAA GAACTATGAG TTCATGTGGA ATCCTCACCT GGGCTACATC 
CTCACATGCC CATCCAACCT GGGCACCGGA CTGCGGGCAG GTGTGCACAT CAAGCTGCCC 
CACCTGGGGA AGCACGAGAA GTTCTCGGAG GTGCTCAAGC GGCTGCGGCT TCAGAAGCGA 
GGCACAGGTG GCGTGGACAC CGCTGCTGTC GGTGGGGTGT TTGATGTCTC CAACGCTGAC 
CGCCTGGGCT TCTCGGAGGT GGAACTGGTG CAGATGGTGG TGGACGGAGT GAAACTACTC 
ATTGAGATGG AGCAACGGCT GGAGCAGGGT CAGGCAATCG ATGACCTCAT GCCGGCCCAG 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cell-Type-Specific Spatiotemporal Expression of Creatine Biosynthetic Enzyme S-adenosylmethionine:guanidinoacetate N-methyltransferase in Developing Mouse Brain.
Neurochemical research(2017 Dec)
Tachikawa M,Watanabe M,Fukaya M,Sakai K,Terasaki T,Hosoya KI

Creatine kinase B is necessary to limit myoblast fusion during myogenesis.
American journal of physiology. Cell physiology308(11)C919-31(2015 Jun)
Simionescu-Bankston A,Pichavant C,Canner JP,Apponi LH,Wang Y,Steeds C,Olthoff JT,Belanto JJ,Ervasti JM,Pavlath GK

Analysis of Presenilin 1 and 2 interacting proteins in mouse cerebral cortex during development.
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience38138-46(2014 Nov)
Kumar A,Thakur MK

Isoaspartyl formation in creatine kinase B is associated with loss of enzymatic activity; implications for the linkage of isoaspartate accumulation and neurological dysfunction in the PIMT knockout mouse.
PloS one9(6)e100622(2014)
Dimitrijevic A,Qin Z,Aswad DW

Genetic control of autoimmune myocarditis mediated by myosin-specific antibodies.
Immunogenetics49(2)79-85(1999 Feb)
Kuan AP,Chamberlain W,Malkiel S,Lieu HD,Factor SM,Diamond B,Kotzin BL

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Ckb cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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