Bub1b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bub1b
Entrez Gene ID	12236
Full Name	BUB1B, mitotic checkpoint serine/threonine kinase
Synonyms	AU045529,BUBR1
General protein information	Preferred Names BUB1B, mitotic checkpoint serine/threonine kinase Names mitotic checkpoint serine/threonine-protein kinase BUB1 beta MAD3/BUB1-related protein kinase budding uninhibited by benzimidazoles 1 beta budding uninhibited by benzimidazoles 1 homolog, beta mitotic checkpoint kinase MAD3L
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 2 Map Location: 2\|2 E5

mRNA and Protein(s)

mRNA	Protein	Name
NM_009773.3	NP_033903.2	mitotic checkpoint serine/threonine-protein kinase BUB1 beta
XM_006498622.3	XP_006498685.1	mitotic checkpoint serine/threonine-protein kinase BUB1 beta isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	BUB1B	NP_001202.4
Pan troglodytes (chimpanzee)	BUB1B	XP_510299.3
	BUB1B	XP_002804771.1
Canis lupus familiaris (dog)	BUB1B	XP_850443.2
Gallus gallus (chicken)	BUB1B	NP_989734.1
Bos taurus (cattle)	BUB1B	NP_001138645.1
Arabidopsis thaliana (thale cress)	BUBR1	NP_565767.1
Oryza sativa (rice)	Os02g0193600	NP_001046171.1
Mus musculus (house mouse)	Bub1b	NP_033903.2
Rattus norvegicus (Norway rat)	Bub1b	XP_342495.4
Danio rerio (zebrafish)	bub1bb	XP_695384.3
Xenopus tropicalis (tropical clawed frog)	bub1b	NP_001096484.1

Gene Ontology
Bibliography

Related articles in PubMed

Sirt2-BubR1 acetylation pathway mediates the effects of advanced maternal age on oocyte quality.
Qiu D, Hou X, Han L, Li X, Ge J, Wang Q
Aging cell17(1)(2018 Feb)

BubR1 insufficiency impairs angiogenesis in aging and in experimental critical limb ischemic mice.
Okadome J, Matsumoto T, Yoshiya K, Matsuda D, Tamada K, Onimaru M, Nakano K, Egashira K, Yonemitsu Y, Maehara Y
Journal of vascular surgery(2017 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Age-related decline in BubR1 impairs adult hippocampal neurogenesis.
Title: Age-related decline in BubR1 impairs adult hippocampal neurogenesis.

EZH2 directly interacted with and stabilized BubR1, an effect driving EZH2 into the concert of meiosis regulation.
Title: EZH2 is required for mouse oocyte meiotic maturation by interacting with and stabilizing spindle assembly checkpoint protein BubRI.

Consistent with defective myelination, BubR1(H/H) mice exhibited various motor deficits, including impaired motor strength, coordination, and balance, irregular gait patterns and reduced locomotor activity.
Title: The progeroid gene BubR1 regulates axon myelination and motor function.

Data show that compound mutant spindle assembly checkpoint components BubR1 and Sgo1 embryonic fibroblasts (MEFs) grew at a much slower rate, and a small fraction of cells exhibited morphologies of senescent cells at early passages.
Title: Haplo-insufficiency of both BubR1 and SGO1 accelerates cellular senescence.

we quantified the frequency of aneuploidy of three autosomes in the cerebral cortex and cerebellum of adult and developing brain of Bub1b(H/H) mice, which have a faulty mitotic checkpoint, and Ercc1(-/Delta7) mice, defective in nucleotide excision repair and inter-strand cross-link repair.we found that Bub1b(H/H), but not Ercc1(-/Delta7) mice, have a significantly higher frequency of aneuploid nuclei relative to wild-t...
Title: Whole chromosome aneuploidy in the brain of Bub1bH/H and Ercc1-/?7 mice.

The following Bub1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bub1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu21997	NM_009773.3 Latest version!	Mus musculus BUB1B, mitotic checkpoint serine/threonine kinase (Bub1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu29943	XM_006498622.3 Latest version!	Mus musculus BUB1B, mitotic checkpoint serine/threonine kinase (Bub1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bub1b ( NM_009773.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033903.2 Mus musculus BUB1B, mitotic checkpoint serine/threonine kinase (Bub1b), mRNA.

Price & Availability↑

CloneID	OMu21997
Clone ID Related Accession (Same CDS sequence)	NM_009773.3
Accession Version	NM_009773.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3159bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 beta
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY013487.1, AK133909.1, BU054510.1 and AV315589.1. On Oct 6, 2007 this sequence version replaced NM_009773.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF107296.1, BC031577.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849377, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGG CGAGTGAAGC CATGTGCCTG GAGGGAGCAG AGTGGGAGCT GAGTAAAGAA 
AACATACAGC CCTTACGGCA CGGGCGGGTC ATGTCCACAC TTCAGGGAGC TTTGGCAAAG 
CAAGAGTCAG CCGGCCACAC TGCTCTGCAG CAGCAGAAAC GGGCATTTGA ATCTGAAATC 
CGCTTTTACT CTGGAGATGA CCCTCTGGAT GTGTGGGACA GATATATTAA TTGGACAGAA 
CAGAACTACC CTCAAGGGGG GAAAGAGAGT AACATGTCAG CGTTAGTGGA GAGAGCGATA 
GAAGCACTCC AAGGAGAGAC GCGCTATTAT AATGACCCCC GCTTTCTCAG TCTCTGGATC 
AAATTGGGAC ATTTGTGCAA TGAACCTTTG GATATGTACA GCTATTTACA AAGCCAAGGA 
ATTGGCGTTT CCCTTGCCCA GTTCTATATT TCATGGGCTG AAGAATACGA AGCTAGAGAA 
AATTTCAAGA AAGCGGACAT AATATTCCAG GAAGGGATTG AACGCAAGGC TGAGCCCCTG 
GACAGACTGC AGTCCCAGCA CAGACAGTTC CAGTCTCGAG TGTCGCGACA AGCTTTCTTG 
GCCCTTGGGA ATGAAGAGGA GGAGGCTTTG GAGCCTTCTG AACCACAGAG AAGCTCGCTA 
GCTGAGCTGA AGAGCAGAGG GAAGAAGATG GCCAGAGCGC CCATCAGCCG TGTCGGAGGT 
GCTCTGAAAG CTCCAGGTCA GAGCAGAGGG TTCCTAAATG CAGTTCCCCA GCCAGTACAC 
GGTAATCGCA GGATCACCGT TTTTGATGAA AATGCCGATA CCGCGTCTAG AACGGAGTTA 
TCTAAGCCTG TAGCCCAGCC ATGGATGGCA CCCCCTGTGC CCAGGGCCAA AGAGAACGAA 
CTTCAGCCAG GCCCATGGAG CACAGACAGG CCGGCGGGAC GCAGGCCTCA TGACAATCCA 
GCCTCTGTGA CGTCGATTCC CAGCGTGCTT CCCAGCTTTA CGCCGTACGT GGAAGAGAGC 
GCCCAGCAGA CAGTCATGAC ACCATGCAAG ATTGAGCCTA GTATCAACCA TGTTCTCAGC 
ACCAGGAAGC CAGGGAGAGA AGAAGGAGAC CCCTTGCAGA GAGTTCAGAG TCATCAGCAA 
GGCTGTGAGG AGAAGAAGGA GAAGATGATG TACTGTAAGG AGAAGATCTA TGCCGGAGTT 
GGGGAGTTCT CCTTTGAGGA GATCCGAGCT GAAGTGTTCC GAAAGAAGCT GAAAGAACGA 
AGGGAAGCCG AGCTGTTGAC CAGTGCAAAG AAGAGGGAGG AGATGCAGAA GCAGATCGAA 
GAGATGGAGA GGAGGCTGAA GGCAATGCAG GCTGTTCAGC AAGAAGGAGC TGGGGGCCAG 
CAAGAAGAGA AGATGCCTAC AGAGGACCCA GCCAGATTGC AGATTGCTTC GGGGCCTCAG 
GAAATGTCGG GAGTTCCTCT GTCCTGTTCC ATCTGTCCAC TAAGCTCGAA TCCTAGGGAA 
ATTTCACCTG CTGAGAACAT TTTGCAAGAA CAGCCTGATT CTAAAGGTTC CAGTATGCCT 
TTCTCCATTT TTGATGAGTC TCTTTCAGAC AAAAAGGACA AAAGTCCTGC TACAGGTGGT 
CCACAGGTTC TCAATGCCCA GAGAAGACCC CTTTCAGTTC TCAAAACTAC AGAAGTGGGC 
ACCACAAATG AGGATGTGTC TCCCGATATT TGTGATGAAC TCACAGAACT TGAGCCTCTG 
AGTGAAGACG CCATCATCAC TGGTTTCAGG AACGTCACTC TCTGTCCCAA CCCTGAGGAC 
ACTTGTGACT TTGCTAGAGC AGCTCGTTTG GCATCTACTC CTTTCCATGA GATACTGTCC 
TCGAAGGGCA TCGCTGCTGA TCCCGAGGGA CTGTTGCAGG AAGAGGATCT GGATGGGAAG 
GCCGCCGAGG CTCATCACAC TGTTCATCAC CAGGCCCTCA TCATAAAGAA ACTGAGCCCA 
ATTATTGAAG AGAGCCGTGA GGCCACCCAC TCATCTGGCT TCTCCAGGTC TTCTTCCTCA 
GCTCCCAGTA CATCCTCCAT CAAAGGCTTT CAGCTTCTGG AAAAGCTGGA GCTGACTAAT 
GACGGGGCAG AAGATGCTAT TCAGTCACCC TGGTGTTCAC AGTATCGCCT ACAACTGTTA 
AAATCCCTAC TAGAATTAAG TGCTTTTGCG GAGTTTTCTG TGGAAGACCG ACCGATGCCT 
GTGCTGGAAA TAGGGAAGGA GATTGAGTTA GGTCCTGAGG ATTACGTCAT CAAGCAAGAG 
CACCTAACAT GTGACGATTA CAGGTTATTC TGGGTGGCAC CAAGAAGCTC TGCAGAGCTA 
ACCATGATAA AGGCATCATC TCAGCCTATC CCGTGGGATT TTTATATCAA CCTCAAGTTG 
AAGGAGCGTC TGAATGAGGA CTATGACCAG CTTTGCAGCT GCTGTCAGTA CCAAGATGGC 
CATGTTGTTT GGTACCAGTA TATAAACTGC TCCACCCTTC AGAATCTTCT CCAACACAGC 
GAATTTGTTA CTCATGAAAT AATAGTGTTG ATTATTTACA ACCTCTTGAC AATCGTGGAG 
AAGCTACACA GAGCTGAAAT AGTACACGGA GACTTGAGTC CACGGAGTCT GATCCTACGA 
AACAGAATCC ACGACCCCTA TGACTATGTA AATAAGGACG ATCACGCTGT GAGGATCATG 
GACTTCTCCT ACAGTGTTGA CCTGAGGGTG CAGCTGGATG CGTTTGCCTA TAGTGGCTTT 
CGGACTGCAC AGATCCTGGA AGGACAAAAG ATCCTGGCTA ACTGTTCTTC TCCCTACCAT 
GTAGATCTGT TGGGTATAGC AGACCTAGCG CACTTACTCC TGTTCAAGGA GCACCTCCAT 
GTCTTCTGGG ATGGACTCCT CTGGAAACTT AGCCAGAGCA CCTCTGAGCT AAAAGACAGT 
GAATTGTGGA ATAAATTCTT TGTGCGGATT CTGAATGCCA GTGACAAGTC CACAGTGTCT 
GTTCTGGGGG AGCTGGCAGC AGAAATGGGT GGGGCTTTTG ATGCCACATT CCATAGCCAC 
CTGAACAGAG CCCTGTGGAA GCTGGGGAAG ACAATCAGCC CGGAAGCTTT GCTCACTCAG 
CAAGACAAGC AGCCAGGCGG CTCCCAGAGC CCTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033903.2
CDS	238..3396
Translation	MAEASEAMCLEGAEWELSKENIQPLRHGRVMSTLQGALAKQESAGHTALQQQKRAFESEIRFYSGDDPLDVWDRYINWTEQNYPQGGKESNMSALVERAIEALQGETRYYNDPRFLSLWIKLGHLCNEPLDMYSYLQSQGIGVSLAQFYISWAEEYEARENFKKADIIFQEGIERKAEPLDRLQSQHRQFQSRVSRQAFLALGNEEEEALEPSEPQRSSLAELKSRGKKMARAPISRVGGALKAPGQSRGFLNAVPQPVHGNRRITVFDENADTASRTELSKPVAQPWMAPPVPRAKENELQPGPWSTDRPAGRRPHDNPASVTSIPSVLPSFTPYVEESAQQTVMTPCKIEPSINHVLSTRKPGREEGDPLQRVQSHQQGCEEKKEKMMYCKEKIYAGVGEFSFEEIRAEVFRKKLKERREAELLTSAKKREEMQKQIEEMERRLKAMQAVQQEGAGGQQEEKMPTEDPARLQIASGPQEMSGVPLSCSICPLSSNPREISPAENILQEQPDSKGSSMPFSIFDESLSDKKDKSPATGGPQVLNAQRRPLSVLKTTEVGTTNEDVSPDICDELTELEPLSEDAIITGFRNVTLCPNPEDTCDFARAARLASTPFHEILSSKGIAADPEGLLQEEDLDGKAAEAHHTVHHQALIIKKLSPIIEESREATHSSGFSRSSSSAPSTSSIKGFQLLEKLELTNDGAEDAIQSPWCSQYRLQLLKSLLELSAFAEFSVEDRPMPVLEIGKEIELGPEDYVIKQEHLTCDDYRLFWVAPRSSAELTMIKASSQPIPWDFYINLKLKERLNEDYDQLCSCCQYQDGHVVWYQYINCSTLQNLLQHSEFVTHEIIVLIIYNLLTIVEKLHRAEIVHGDLSPRSLILRNRIHDPYDYVNKDDHAVRIMDFSYSVDLRVQLDAFAYSGFRTAQILEGQKILANCSSPYHVDLLGIADLAHLLLFKEHLHVFWDGLLWKLSQSTSELKDSELWNKFFVRILNASDKSTVSVLGELAAEMGGAFDATFHSHLNRALWKLGKTISPEALLTQQDKQPGGSQSPA

Target ORF information:

RefSeq Version	NM_009773.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus BUB1B, mitotic checkpoint serine/threonine kinase (Bub1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009773.3

ORF Insert Sequence:

ATGGCGGAGG CGAGTGAAGC CATGTGCCTG GAGGGAGCAG AGTGGGAGCT GAGTAAAGAA 
AACATACAGC CCTTACGGCA CGGGCGGGTC ATGTCCACAC TTCAGGGAGC TTTGGCAAAG 
CAAGAGTCAG CCGGCCACAC TGCTCTGCAG CAGCAGAAAC GGGCATTTGA ATCTGAAATC 
CGCTTTTACT CTGGAGATGA CCCTCTGGAT GTGTGGGACA GATATATTAA TTGGACAGAA 
CAGAACTACC CTCAAGGGGG GAAAGAGAGT AACATGTCAG CGTTAGTGGA GAGAGCGATA 
GAAGCACTCC AAGGAGAGAC GCGCTATTAT AATGACCCCC GCTTTCTCAG TCTCTGGATC 
AAATTGGGAC ATTTGTGCAA TGAACCTTTG GATATGTACA GCTATTTACA AAGCCAAGGA 
ATTGGCGTTT CCCTTGCCCA GTTCTATATT TCATGGGCTG AAGAATACGA AGCTAGAGAA 
AATTTCAAGA AAGCGGACAT AATATTCCAG GAAGGGATTG AACGCAAGGC TGAGCCCCTG 
GACAGACTGC AGTCCCAGCA CAGACAGTTC CAGTCTCGAG TGTCGCGACA AGCTTTCTTG 
GCCCTTGGGA ATGAAGAGGA GGAGGCTTTG GAGCCTTCTG AACCACAGAG AAGCTCGCTA 
GCTGAGCTGA AGAGCAGAGG GAAGAAGATG GCCAGAGCGC CCATCAGCCG TGTCGGAGGT 
GCTCTGAAAG CTCCAGGTCA GAGCAGAGGG TTCCTAAATG CAGTTCCCCA GCCAGTACAC 
GGTAATCGCA GGATCACCGT TTTTGATGAA AATGCCGATA CCGCGTCTAG AACGGAGTTA 
TCTAAGCCTG TAGCCCAGCC ATGGATGGCA CCCCCTGTGC CCAGGGCCAA AGAGAACGAA 
CTTCAGCCAG GCCCATGGAG CACAGACAGG CCGGCGGGAC GCAGGCCTCA TGACAATCCA 
GCCTCTGTGA CGTCGATTCC CAGCGTGCTT CCCAGCTTTA CGCCGTACGT GGAAGAGAGC 
GCCCAGCAGA CAGTCATGAC ACCATGCAAG ATTGAGCCTA GTATCAACCA TGTTCTCAGC 
ACCAGGAAGC CAGGGAGAGA AGAAGGAGAC CCCTTGCAGA GAGTTCAGAG TCATCAGCAA 
GGCTGTGAGG AGAAGAAGGA GAAGATGATG TACTGTAAGG AGAAGATCTA TGCCGGAGTT 
GGGGAGTTCT CCTTTGAGGA GATCCGAGCT GAAGTGTTCC GAAAGAAGCT GAAAGAACGA 
AGGGAAGCCG AGCTGTTGAC CAGTGCAAAG AAGAGGGAGG AGATGCAGAA GCAGATCGAA 
GAGATGGAGA GGAGGCTGAA GGCAATGCAG GCTGTTCAGC AAGAAGGAGC TGGGGGCCAG 
CAAGAAGAGA AGATGCCTAC AGAGGACCCA GCCAGATTGC AGATTGCTTC GGGGCCTCAG 
GAAATGTCGG GAGTTCCTCT GTCCTGTTCC ATCTGTCCAC TAAGCTCGAA TCCTAGGGAA 
ATTTCACCTG CTGAGAACAT TTTGCAAGAA CAGCCTGATT CTAAAGGTTC CAGTATGCCT 
TTCTCCATTT TTGATGAGTC TCTTTCAGAC AAAAAGGACA AAAGTCCTGC TACAGGTGGT 
CCACAGGTTC TCAATGCCCA GAGAAGACCC CTTTCAGTTC TCAAAACTAC AGAAGTGGGC 
ACCACAAATG AGGATGTGTC TCCCGATATT TGTGATGAAC TCACAGAACT TGAGCCTCTG 
AGTGAAGACG CCATCATCAC TGGTTTCAGG AACGTCACTC TCTGTCCCAA CCCTGAGGAC 
ACTTGTGACT TTGCTAGAGC AGCTCGTTTG GCATCTACTC CTTTCCATGA GATACTGTCC 
TCGAAGGGCA TCGCTGCTGA TCCCGAGGGA CTGTTGCAGG AAGAGGATCT GGATGGGAAG 
GCCGCCGAGG CTCATCACAC TGTTCATCAC CAGGCCCTCA TCATAAAGAA ACTGAGCCCA 
ATTATTGAAG AGAGCCGTGA GGCCACCCAC TCATCTGGCT TCTCCAGGTC TTCTTCCTCA 
GCTCCCAGTA CATCCTCCAT CAAAGGCTTT CAGCTTCTGG AAAAGCTGGA GCTGACTAAT 
GACGGGGCAG AAGATGCTAT TCAGTCACCC TGGTGTTCAC AGTATCGCCT ACAACTGTTA 
AAATCCCTAC TAGAATTAAG TGCTTTTGCG GAGTTTTCTG TGGAAGACCG ACCGATGCCT 
GTGCTGGAAA TAGGGAAGGA GATTGAGTTA GGTCCTGAGG ATTACGTCAT CAAGCAAGAG 
CACCTAACAT GTGACGATTA CAGGTTATTC TGGGTGGCAC CAAGAAGCTC TGCAGAGCTA 
ACCATGATAA AGGCATCATC TCAGCCTATC CCGTGGGATT TTTATATCAA CCTCAAGTTG 
AAGGAGCGTC TGAATGAGGA CTATGACCAG CTTTGCAGCT GCTGTCAGTA CCAAGATGGC 
CATGTTGTTT GGTACCAGTA TATAAACTGC TCCACCCTTC AGAATCTTCT CCAACACAGC 
GAATTTGTTA CTCATGAAAT AATAGTGTTG ATTATTTACA ACCTCTTGAC AATCGTGGAG 
AAGCTACACA GAGCTGAAAT AGTACACGGA GACTTGAGTC CACGGAGTCT GATCCTACGA 
AACAGAATCC ACGACCCCTA TGACTATGTA AATAAGGACG ATCACGCTGT GAGGATCATG 
GACTTCTCCT ACAGTGTTGA CCTGAGGGTG CAGCTGGATG CGTTTGCCTA TAGTGGCTTT 
CGGACTGCAC AGATCCTGGA AGGACAAAAG ATCCTGGCTA ACTGTTCTTC TCCCTACCAT 
GTAGATCTGT TGGGTATAGC AGACCTAGCG CACTTACTCC TGTTCAAGGA GCACCTCCAT 
GTCTTCTGGG ATGGACTCCT CTGGAAACTT AGCCAGAGCA CCTCTGAGCT AAAAGACAGT 
GAATTGTGGA ATAAATTCTT TGTGCGGATT CTGAATGCCA GTGACAAGTC CACAGTGTCT 
GTTCTGGGGG AGCTGGCAGC AGAAATGGGT GGGGCTTTTG ATGCCACATT CCATAGCCAC 
CTGAACAGAG CCCTGTGGAA GCTGGGGAAG ACAATCAGCC CGGAAGCTTT GCTCACTCAG 
CAAGACAAGC AGCCAGGCGG CTCCCAGAGC CCTGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bub1b ( XM_006498622.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006498685.1 Mus musculus BUB1B, mitotic checkpoint serine/threonine kinase (Bub1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu29943
Clone ID Related Accession (Same CDS sequence)	XM_006498622.3
Accession Version	XM_006498622.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000068.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006498622.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGAGG CGAGTGAAGC CATGTGCCTG GAGGGAGCAG AGTGGGAGCT GAGTAAAGAA 
AACATACAGC CCTTACGGCA CGGGCGGGTC ATGTCCACAC TTCAGGGAGC TTTGGCAAAG 
CAAGAGTCAG CCGGCCACAC TGCTCTGCAG CAGCAGAAAC GGGCATTTGA ATCTGAAATC 
CGCTTTTACT CTGGAGATGA CCCTCTGGAT GTGTGGGACA GATATATTAA TTGGACAGAA 
CAGAACTACC CTCAAGGGGG GAAAGAGAGT AACATGTCAG CGTTAGTGGA GAGAGCGATA 
GAAGCACTCC AAGGAGAGAC GCGCTATTAT AATGACCCCC GCTTTCTCAG TCTCTGGATC 
AAATTGGGAC ATTTGTGCAA TGAACCTTTG GATATGTACA GCTATTTACA AAGCCAAGGA 
ATTGGCGTTT CCCTTGCCCA GTTCTATATT TCATGGGCTG AAGAATACGA AGCTAGAGAA 
AATTTCAAGA AAGCGGACAT AATATTCCAG GAAGGGATTG AACGCAAGGC TGAGCCCCTG 
GACAGACTGC AGTCCCAGCA CAGACAGTTC CAGTCTCGAG TGTCGCGACA AGCTTTCTTG 
GCCCTTGGGA ATGAAGAGGA GGAGGCTTTG GAGCCTTCTG AACCACAGAG AAGCTCGCTA 
GCTGAGCTGA AGAGCAGAGG GAAGAAGATG GCCAGAGCGC CCATCAGCCG TGTCGGAGGT 
GCTCTGAAAG CTCCAGGTCA GAGCAGAGGG TTCCTAAATG CAGTTCCCCA GCCAGTACAC 
GGTAATCGCA GGATCACCGT TTTTGATGAA AATGCCGATA CCGCGTCTAG AACGGAGTTA 
TCTAAGCCTG TAGCCCAGCC ATGGATGGCA CCCCCTGTGC CCAGGGCCAA AGAGAACGAA 
CTTCAGCCAG GCCCATGGAG CACAGACAGG CCGGCGGGAC GCAGGCCTCA TGACAATCCA 
GCCTCTGTGA CGTCGATTCC CAGCGTGCTT CCCAGCTTTA CGCCGTACGT GGAAGAGAGC 
GCCCAGCAGA CAGTCATGAC ACCATGCAAG ATTGAGCCTA GTATCAACCA TGTTCTCAGC 
ACCAGGAAGC CAGGGAGAGA AGAAGGAGAC CCCTTGCAGA GAGTTCAGAG TCATCAGCAA 
GGCTGTGAGG AGAAGAAGGA GAAGATGATG TACTGTAAGG AGAAGATCTA TGCCGGAGTT 
GGGGAGTTCT CCTTTGAGGA GATCCGAGCT GAAGTGTTCC GAAAGAAGCT GAAAGAACGA 
AGGGAAGCCG AGCTGTTGAC CAGTGCAAAG AAGAGGGAGG AGATGCAGAA GCAGATCGAA 
GAGATGGAGA GGAGGCTGAA GGCAATGCAG GCTGTTCAGC AAGAAGGAGC TGGGGGCCAG 
CAGCAAGAAG AGAAGATGCC TACAGAGGAC CCAGCCAGAT TGCAGATTGC TTCGGGGCCT 
CAGGAAATGT CGGGAGTTCC TCTGTCCTGT TCCATCTGTC CACTAAGCTC GAATCCTAGG 
GAAATTTCAC CTGCTGAGAA CATTTTGCAA GAACAGCCTG ATTCTAAAGG TTCCAGTATG 
CCTTTCTCCA TTTTTGATGA GTCTCTTTCA GACAAAAAGG ACAAAAGTCC TGCTACAGGT 
GGTCCACAGG TTCTCAATGC CCAGAGAAGA CCCCTTTCAG TTCTCAAAAC TACAGAAGTG 
GGCACCACAA ATGAGGATGT GTCTCCCGAT ATTTGTGATG AACTCACAGA ACTTGAGCCT 
CTGAGTGAAG ACGCCATCAT CACTGGTTTC AGGAACGTCA CTCTCTGTCC CAACCCTGAG 
GACACTTGTG ACTTTGCTAG AGCAGCTCGT TTGGCATCTA CTCCTTTCCA TGAGATACTG 
TCCTCGAAGG GCATCGCTGC TGATCCCGAG GGACTGTTGC AGGAAGAGGA TCTGGATGGG 
AAGGCCGCCG AGGCTCATCA CACTGTTCAT CACCAGGCCC TCATCATAAA GAAACTGAGC 
CCAATTATTG AAGAGAGCCG TGAGGCCACC CACTCATCTG GCTTCTCCAG GTCTTCTTCC 
TCAGCTCCCA GTACATCCTC CATCAAAGGC TTTCAGCTTC TGGAAAAGCT GGAGCTGACT 
AATGACGGGG CAGAAGATGC TATTCAGTCA CCCTGGTGTT CACAGTATCG CCTACAACTG 
TTAAAATCCC TACTAGAATT AAGTGCTTTT GCGGAGTTTT CTGTGGAAGA CCGACCGATG 
CCTGTGCTGG AAATAGGGAA GGAGATTGAG TTAGGTCCTG AGGATTACGT CATCAAGCAA 
GAGCACCTAA CATGTGACGA TTACAGGTTA TTCTGGGTGG CACCAAGAAG CTCTGCAGAG 
CTAACCATGA TAAAGGCATC ATCTCAGCCT ATCCCGTGGG ATTTTTATAT CAACCTCAAG 
TTGAAGGAGC GTCTGAATGA GGACTATGAC CAGCTTTGCA GCTGCTGTCA GTACCAAGAT 
GGCCATGTTG TTTGGTACCA GTATATAAAC TGCTCCACCC TTCAGAATCT TCTCCAACAC 
AGCGAATTTG TTACTCATGA AATAATAGTG TTGATTATTT ACAACCTCTT GACAATCGTG 
GAGAAGCTAC ACAGAGCTGA AATAGTACAC GGAGACTTGA GTCCACGGAG TCTGATCCTA 
CGAAACAGAA TCCACGACCC CTATGACTAT GTAAATAAGG ACGATCACGC TGTGAGGATC 
ATGGACTTCT CCTACAGTGT TGACCTGAGG GTGCAGCTGG ATGCGTTTGC CTATAGTGGC 
TTTCGGACTG CACAGATCCT GGAAGGACAA AAGATCCTGG CTAACTGTTC TTCTCCCTAC 
CATGTAGATC TGTTGGGTAT AGCAGACCTA GCGCACTTAC TCCTGTTCAA GGAGCACCTC 
CATGTCTTCT GGGATGGACT CCTCTGGAAA CTTAGCCAGA GCACCTCTGA GCTAAAAGAC 
AGTGAATTGT GGAATAAATT CTTTGTGCGG ATTCTGAATG CCAGTGACAA GTCCACAGTG 
TCTGTTCTGG GGGAGCTGGC AGCAGAAATG GGTGGGGCTT TTGATGCCAC ATTCCATAGC 
CACCTGAACA GAGCCCTGTG GAAGCTGGGG AAGACAATCA GCCCGGAAGC TTTGCTCACT 
CAGCAAGACA AGCAGCCAGG CGGCTCCCAG AGCCCTGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006498685.1
CDS	247..3408
Translation	MAEASEAMCLEGAEWELSKENIQPLRHGRVMSTLQGALAKQESAGHTALQQQKRAFESEIRFYSGDDPLDVWDRYINWTEQNYPQGGKESNMSALVERAIEALQGETRYYNDPRFLSLWIKLGHLCNEPLDMYSYLQSQGIGVSLAQFYISWAEEYEARENFKKADIIFQEGIERKAEPLDRLQSQHRQFQSRVSRQAFLALGNEEEEALEPSEPQRSSLAELKSRGKKMARAPISRVGGALKAPGQSRGFLNAVPQPVHGNRRITVFDENADTASRTELSKPVAQPWMAPPVPRAKENELQPGPWSTDRPAGRRPHDNPASVTSIPSVLPSFTPYVEESAQQTVMTPCKIEPSINHVLSTRKPGREEGDPLQRVQSHQQGCEEKKEKMMYCKEKIYAGVGEFSFEEIRAEVFRKKLKERREAELLTSAKKREEMQKQIEEMERRLKAMQAVQQEGAGGQQQEEKMPTEDPARLQIASGPQEMSGVPLSCSICPLSSNPREISPAENILQEQPDSKGSSMPFSIFDESLSDKKDKSPATGGPQVLNAQRRPLSVLKTTEVGTTNEDVSPDICDELTELEPLSEDAIITGFRNVTLCPNPEDTCDFARAARLASTPFHEILSSKGIAADPEGLLQEEDLDGKAAEAHHTVHHQALIIKKLSPIIEESREATHSSGFSRSSSSAPSTSSIKGFQLLEKLELTNDGAEDAIQSPWCSQYRLQLLKSLLELSAFAEFSVEDRPMPVLEIGKEIELGPEDYVIKQEHLTCDDYRLFWVAPRSSAELTMIKASSQPIPWDFYINLKLKERLNEDYDQLCSCCQYQDGHVVWYQYINCSTLQNLLQHSEFVTHEIIVLIIYNLLTIVEKLHRAEIVHGDLSPRSLILRNRIHDPYDYVNKDDHAVRIMDFSYSVDLRVQLDAFAYSGFRTAQILEGQKILANCSSPYHVDLLGIADLAHLLLFKEHLHVFWDGLLWKLSQSTSELKDSELWNKFFVRILNASDKSTVSVLGELAAEMGGAFDATFHSHLNRALWKLGKTISPEALLTQQDKQPGGSQSPA

Target ORF information:

RefSeq Version	XM_006498622.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus BUB1B, mitotic checkpoint serine/threonine kinase (Bub1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006498622.3

ORF Insert Sequence:

ATGGCGGAGG CGAGTGAAGC CATGTGCCTG GAGGGAGCAG AGTGGGAGCT GAGTAAAGAA 
AACATACAGC CCTTACGGCA CGGGCGGGTC ATGTCCACAC TTCAGGGAGC TTTGGCAAAG 
CAAGAGTCAG CCGGCCACAC TGCTCTGCAG CAGCAGAAAC GGGCATTTGA ATCTGAAATC 
CGCTTTTACT CTGGAGATGA CCCTCTGGAT GTGTGGGACA GATATATTAA TTGGACAGAA 
CAGAACTACC CTCAAGGGGG GAAAGAGAGT AACATGTCAG CGTTAGTGGA GAGAGCGATA 
GAAGCACTCC AAGGAGAGAC GCGCTATTAT AATGACCCCC GCTTTCTCAG TCTCTGGATC 
AAATTGGGAC ATTTGTGCAA TGAACCTTTG GATATGTACA GCTATTTACA AAGCCAAGGA 
ATTGGCGTTT CCCTTGCCCA GTTCTATATT TCATGGGCTG AAGAATACGA AGCTAGAGAA 
AATTTCAAGA AAGCGGACAT AATATTCCAG GAAGGGATTG AACGCAAGGC TGAGCCCCTG 
GACAGACTGC AGTCCCAGCA CAGACAGTTC CAGTCTCGAG TGTCGCGACA AGCTTTCTTG 
GCCCTTGGGA ATGAAGAGGA GGAGGCTTTG GAGCCTTCTG AACCACAGAG AAGCTCGCTA 
GCTGAGCTGA AGAGCAGAGG GAAGAAGATG GCCAGAGCGC CCATCAGCCG TGTCGGAGGT 
GCTCTGAAAG CTCCAGGTCA GAGCAGAGGG TTCCTAAATG CAGTTCCCCA GCCAGTACAC 
GGTAATCGCA GGATCACCGT TTTTGATGAA AATGCCGATA CCGCGTCTAG AACGGAGTTA 
TCTAAGCCTG TAGCCCAGCC ATGGATGGCA CCCCCTGTGC CCAGGGCCAA AGAGAACGAA 
CTTCAGCCAG GCCCATGGAG CACAGACAGG CCGGCGGGAC GCAGGCCTCA TGACAATCCA 
GCCTCTGTGA CGTCGATTCC CAGCGTGCTT CCCAGCTTTA CGCCGTACGT GGAAGAGAGC 
GCCCAGCAGA CAGTCATGAC ACCATGCAAG ATTGAGCCTA GTATCAACCA TGTTCTCAGC 
ACCAGGAAGC CAGGGAGAGA AGAAGGAGAC CCCTTGCAGA GAGTTCAGAG TCATCAGCAA 
GGCTGTGAGG AGAAGAAGGA GAAGATGATG TACTGTAAGG AGAAGATCTA TGCCGGAGTT 
GGGGAGTTCT CCTTTGAGGA GATCCGAGCT GAAGTGTTCC GAAAGAAGCT GAAAGAACGA 
AGGGAAGCCG AGCTGTTGAC CAGTGCAAAG AAGAGGGAGG AGATGCAGAA GCAGATCGAA 
GAGATGGAGA GGAGGCTGAA GGCAATGCAG GCTGTTCAGC AAGAAGGAGC TGGGGGCCAG 
CAGCAAGAAG AGAAGATGCC TACAGAGGAC CCAGCCAGAT TGCAGATTGC TTCGGGGCCT 
CAGGAAATGT CGGGAGTTCC TCTGTCCTGT TCCATCTGTC CACTAAGCTC GAATCCTAGG 
GAAATTTCAC CTGCTGAGAA CATTTTGCAA GAACAGCCTG ATTCTAAAGG TTCCAGTATG 
CCTTTCTCCA TTTTTGATGA GTCTCTTTCA GACAAAAAGG ACAAAAGTCC TGCTACAGGT 
GGTCCACAGG TTCTCAATGC CCAGAGAAGA CCCCTTTCAG TTCTCAAAAC TACAGAAGTG 
GGCACCACAA ATGAGGATGT GTCTCCCGAT ATTTGTGATG AACTCACAGA ACTTGAGCCT 
CTGAGTGAAG ACGCCATCAT CACTGGTTTC AGGAACGTCA CTCTCTGTCC CAACCCTGAG 
GACACTTGTG ACTTTGCTAG AGCAGCTCGT TTGGCATCTA CTCCTTTCCA TGAGATACTG 
TCCTCGAAGG GCATCGCTGC TGATCCCGAG GGACTGTTGC AGGAAGAGGA TCTGGATGGG 
AAGGCCGCCG AGGCTCATCA CACTGTTCAT CACCAGGCCC TCATCATAAA GAAACTGAGC 
CCAATTATTG AAGAGAGCCG TGAGGCCACC CACTCATCTG GCTTCTCCAG GTCTTCTTCC 
TCAGCTCCCA GTACATCCTC CATCAAAGGC TTTCAGCTTC TGGAAAAGCT GGAGCTGACT 
AATGACGGGG CAGAAGATGC TATTCAGTCA CCCTGGTGTT CACAGTATCG CCTACAACTG 
TTAAAATCCC TACTAGAATT AAGTGCTTTT GCGGAGTTTT CTGTGGAAGA CCGACCGATG 
CCTGTGCTGG AAATAGGGAA GGAGATTGAG TTAGGTCCTG AGGATTACGT CATCAAGCAA 
GAGCACCTAA CATGTGACGA TTACAGGTTA TTCTGGGTGG CACCAAGAAG CTCTGCAGAG 
CTAACCATGA TAAAGGCATC ATCTCAGCCT ATCCCGTGGG ATTTTTATAT CAACCTCAAG 
TTGAAGGAGC GTCTGAATGA GGACTATGAC CAGCTTTGCA GCTGCTGTCA GTACCAAGAT 
GGCCATGTTG TTTGGTACCA GTATATAAAC TGCTCCACCC TTCAGAATCT TCTCCAACAC 
AGCGAATTTG TTACTCATGA AATAATAGTG TTGATTATTT ACAACCTCTT GACAATCGTG 
GAGAAGCTAC ACAGAGCTGA AATAGTACAC GGAGACTTGA GTCCACGGAG TCTGATCCTA 
CGAAACAGAA TCCACGACCC CTATGACTAT GTAAATAAGG ACGATCACGC TGTGAGGATC 
ATGGACTTCT CCTACAGTGT TGACCTGAGG GTGCAGCTGG ATGCGTTTGC CTATAGTGGC 
TTTCGGACTG CACAGATCCT GGAAGGACAA AAGATCCTGG CTAACTGTTC TTCTCCCTAC 
CATGTAGATC TGTTGGGTAT AGCAGACCTA GCGCACTTAC TCCTGTTCAA GGAGCACCTC 
CATGTCTTCT GGGATGGACT CCTCTGGAAA CTTAGCCAGA GCACCTCTGA GCTAAAAGAC 
AGTGAATTGT GGAATAAATT CTTTGTGCGG ATTCTGAATG CCAGTGACAA GTCCACAGTG 
TCTGTTCTGG GGGAGCTGGC AGCAGAAATG GGTGGGGCTT TTGATGCCAC ATTCCATAGC 
CACCTGAACA GAGCCCTGTG GAAGCTGGGG AAGACAATCA GCCCGGAAGC TTTGCTCACT 
CAGCAAGACA AGCAGCCAGG CGGCTCCCAG AGCCCTGCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sirt2-BubR1 acetylation pathway mediates the effects of advanced maternal age on oocyte quality.
Aging cell17(1)(2018 Feb)
Qiu D,Hou X,Han L,Li X,Ge J,Wang Q

BubR1 insufficiency impairs angiogenesis in aging and in experimental critical limb ischemic mice.
Journal of vascular surgery(2017 Sep)
Okadome J,Matsumoto T,Yoshiya K,Matsuda D,Tamada K,Onimaru M,Nakano K,Egashira K,Yonemitsu Y,Maehara Y

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Bub1b cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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