BUB1B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	BUB1B
Entrez Gene ID	701
Full Name	BUB1 mitotic checkpoint serine/threonine kinase B
Synonyms	BUB1beta,BUBR1,Bub1A,MAD3L,MVA1,SSK1,hBUBR1
General protein information	Preferred Names BUB1 mitotic checkpoint serine/threonine kinase B Names mitotic checkpoint serine/threonine-protein kinase BUB1 beta BUB1B, mitotic checkpoint serine/threonine kinase MAD3/BUB1-related protein kinase budding uninhibited by benzimidazoles 1 homolog beta mitotic checkpoint kinase MAD3L
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q15.1
Summary	This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. [provided by RefSeq, Jul 2008].
Disorder MIM:	602860
Disorder Html:	Colorectal cancer, somatic, 114500 (3); Mosaic variegated aneuploidy syndrome 1, 257300 (3); [Premature chromatid separation trait], 176430 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001211.5	NP_001202.4	mitotic checkpoint serine/threonine-protein kinase BUB1 beta
NM_001211.6	NP_001202.5	mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Pathways from BioSystems

Homologies

Homo sapiens (human)	BUB1B	NP_001202.4
Pan troglodytes (chimpanzee)	BUB1B	XP_510299.3
	BUB1B	XP_002804771.1
Canis lupus familiaris (dog)	BUB1B	XP_850443.2
Gallus gallus (chicken)	BUB1B	NP_989734.1
Bos taurus (cattle)	BUB1B	NP_001138645.1
Arabidopsis thaliana (thale cress)	BUBR1	NP_565767.1
Oryza sativa (rice)	Os02g0193600	NP_001046171.1
Mus musculus (house mouse)	Bub1b	NP_033903.2
Rattus norvegicus (Norway rat)	Bub1b	XP_342495.4
Danio rerio (zebrafish)	bub1bb	XP_695384.3
Xenopus tropicalis (tropical clawed frog)	bub1b	NP_001096484.1

Gene Ontology
Bibliography

Related articles in PubMed

The FOXM1/BUB1B signaling pathway is essential for the tumorigenicity and radioresistance of glioblastoma.
Ma Q, Liu Y, Shang L, Yu J, Qu Q
Oncology reports38(6)3367-3375(2017 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that BRCA2 was required for HDAC2/3 association with acetylated BubR1 in nocodazole (Noc)-arrested cells.
Title: HDAC2/3 binding and deacetylation of BubR1 initiates spindle assembly checkpoint silencing.

Low BUB1B expression is associated with Chromophobe Renal Cell Carcinomas.
Title: HNF1B Loss Exacerbates the Development of Chromophobe Renal Cell Carcinomas.

Results from phylogenomic study identified of a novel conserved cassette of short linear motifs in BubR1 essential for the spindle checkpoint
Title: Phylogenomics-guided discovery of a novel conserved cassette of short linear motifs in BubR1 essential for the spindle checkpoint.

BubR1 N-terminal domain was necessary, but not sufficient to protect against aneuploidy and cancer. In contrast, BubR1 lacking the internal Cdc20-binding domain provided protection against both, which coincided with improved microtubule-kinetochore attachment error correction and spindle assembly checkpoint activity.
Title: BubR1 alterations that reinforce mitotic surveillance act against aneuploidy and cancer.

structure of the PP2A B56-BubR1 complex provides important insights into how the B56 subunit directs the recruitment of PP2A to specific targets.
Title: Crystal structure of a PP2A B56-BubR1 complex and its implications for PP2A substrate recruitment and localization.

The following BUB1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BUB1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu19367	NM_001211.5 Latest version!	Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu109353	NM_001211.6 Latest version!	Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BUB1B ( NM_001211.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001202.4 Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), mRNA.

Price & Availability↑

CloneID	OHu19367
Clone ID Related Accession (Same CDS sequence)	NM_001211.5
Accession Version	NM_001211.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-27
Organism	Homo sapiens(human)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 beta
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025429.6, AF053306.1 and AI742619.1. This sequence is a reference standard in the RefSeqGene project. On Feb 21, 2008 this sequence version replaced NM_001211.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF053306.1, AB208782.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG TGAAGAAGGA AGGGGGTGCT CTGAGTGAAG CCATGTCCCT GGAGGGAGAT 
GAATGGGAAC TGAGTAAAGA AAATGTACAA CCTTTAAGGC AAGGGCGGAT CATGTCCACG 
CTTCAGGGAG CACTGGCACA AGAATCTGCC TGTAACAATA CTCTTCAGCA GCAGAAACGG 
GCATTTGAAT ATGAAATTCG ATTTTACACT GGAAATGACC CTCTGGATGT TTGGGATAGG 
TATATCAGCT GGACAGAGCA GAACTATCCT CAAGGTGGGA AGGAGAGTAA TATGTCAACG 
TTATTAGAAA GAGCTGTAGA AGCACTACAA GGAGAAAAAC GATATTATAG TGATCCTCGA 
TTTCTCAATC TCTGGCTTAA ATTAGGGCGT TTATGCAATG AGCCTTTGGA TATGTACAGT 
TACTTGCACA ACCAAGGGAT TGGTGTTTCA CTTGCTCAGT TCTATATCTC ATGGGCAGAA 
GAATATGAAG CTAGAGAAAA CTTTAGGAAA GCAGATGCGA TATTTCAGGA AGGGATTCAA 
CAGAAGGCTG AACCACTAGA AAGACTACAG TCCCAGCACC GACAATTCCA AGCTCGAGTG 
TCTCGGCAAA CTCTGTTGGC ACTTGAGAAA GAAGAAGAGG AGGAAGTTTT TGAGTCTTCT 
GTACCACAAC GAAGCACACT AGCTGAACTA AAGAGCAAAG GGAAAAAGAC AGCAAGAGCT 
CCAATCATCC GTGTAGGAGG TGCTCTCAAG GCTCCAAGCC AGAACAGAGG ACTCCAAAAT 
CCATTTCCTC AACAGATGCA AAATAATAGT AGAATTACTG TTTTTGATGA AAATGCTGAT 
GAGGCTTCTA CAGCAGAGTT GTCTAAGCCT ACAGTCCAGC CATGGATAGC ACCCCCCATG 
CCCAGGGCCA AAGAGAATGA GCTGCAAGCA GGCCCTTGGA ACACAGGCAG GTCCTTGGAA 
CACAGGCCTC GTGGCAATAC AGCTTCACTG ATAGCTGTAC CCGCTGTGCT TCCCAGTTTC 
ACTCCATATG TGGAAGAGAC TGCACAACAG CCAGTTATGA CACCATGTAA AATTGAACCT 
AGTATAAACC ACATCCTAAG CACCAGAAAG CCTGGAAAGG AAGAAGGAGA TCCTCTACAA 
AGGGTTCAGA GCCATCAGCA AGCGTCTGAG GAGAAGAAAG AGAAGATGAT GTATTGTAAG 
GAGAAGATTT ATGCAGGAGT AGGGGAATTC TCCTTTGAAG AAATTCGGGC TGAAGTTTTC 
CGGAAGAAAT TAAAAGAGCA AAGGGAAGCC GAGCTATTGA CCAGTGCAGA GAAGAGAGCA 
GAAATGCAGA AACAGATTGA AGAGATGGAG AAGAAGCTAA AAGAAATCCA AACTACTCAG 
CAAGAAAGAA CAGGTGATCA GCAAGAAGAG ACGATGCCTA CAAAGGAGAC AACTAAACTG 
CAAATTGCTT CCGAGTCTCA GAAAATACCA GGAATGACTC TATCCAGTTC TGTTTGTCAA 
GTAAACTGTT GTGCCAGAGA AACTTCACTT GCGGAGAACA TTTGGCAGGA ACAACCTCAT 
TCTAAAGGTC CCAGTGTACC TTTCTCCATT TTTGATGAGT TTCTTCTTTC AGAAAAGAAG 
AATAAAAGTC CTCCTGCAGA TCCCCCACGA GTTTTAGCTC AACGAAGACC CCTTGCAGTT 
CTCAAAACCT CAGAAAGCAT CACCTCAAAT GAAGATGTGT CTCCAGATGT TTGTGATGAA 
TTTACAGGAA TTGAACCCTT GAGCGAGGAT GCCATTATCA CAGGCTTCAG AAATGTAACA 
ATTTGTCCTA ACCCAGAAGA CACTTGTGAC TTTGCCAGAG CAGCTCGTTT TGTATCCACT 
CCTTTTCATG AGATAATGTC CTTGAAGGAT CTCCCTTCTG ATCCTGAGAG ACTGTTACCG 
GAAGAAGATC TAGATGTAAA GACCTCTGAG GACCAGCAGA CAGCTTGTGG CACTATCTAC 
AGTCAGACTC TCAGCATCAA GAAGCTGAGC CCAATTATTG AAGACAGTCG TGAAGCCACA 
CACTCCTCTG GCTTCTCTGG TTCTTCTGCC TCGGTTGCAA GCACCTCCTC CATCAAATGT 
CTTCAAATTC CTGAGAAACT AGAACTTACT AATGAGACTT CAGAAAACCC TACTCAGTCA 
CCATGGTGTT CACAGTATCG CAGACAGCTA CTGAAGTCCC TACCAGAGTT AAGTGCCTCT 
GCAGAGTTGT GTATAGAAGA CAGACCAATG CCTAAGTTGG AAATTGAGAA GGAAATTGAA 
TTAGGTAATG AGGATTACTG CATTAAACGA GAATACCTAA TATGTGAAGA TTACAAGTTA 
TTCTGGGTGG CGCCAAGAAA CTCTGCAGAA TTAACAGTAA TAAAGGTATC TTCTCAACCT 
GTCCCATGGG ACTTTTATAT CAACCTCAAG TTAAAGGAAC GTTTAAATGA AGATTTTGAT 
CATTTTTGCA GCTGTTATCA ATATCAAGAT GGCTGTATTG TTTGGCACCA ATATATAAAC 
TGCTTCACCC TTCAGGATCT TCTCCAACAC AGTGAATATA TTACCCATGA AATAACAGTG 
TTGATTATTT ATAACCTTTT GACAATAGTG GAGATGCTAC ACAAAGCAGA AATAGTCCAT 
GGTGACTTGA GTCCAAGGTG TCTGATTCTC AGAAACAGAA TCCACGATCC CTATGATTGT 
AACAAGAACA ATCAAGCTTT GAAGATAGTG GACTTTTCCT ACAGTGTTGA CCTTAGGGTG 
CAGCTGGATG TTTTTACCCT CAGCGGCTTT CGGACTGTAC AGATCCTGGA AGGACAAAAG 
ATCCTGGCTA ACTGTTCTTC TCCCTACCAG GTAGACCTGT TTGGTATAGC AGATTTAGCA 
CATTTACTAT TGTTCAAGGA ACACCTACAG GTCTTCTGGG ATGGGTCCTT CTGGAAACTT 
AGCCAAAATA TTTCTGAGCT AAAAGATGGT GAATTGTGGA ATAAATTCTT TGTGCGGATT 
CTGAATGCCA ATGATGAGGC CACAGTGTCT GTTCTTGGGG AGCTTGCAGC AGAAATGAAT 
GGGGTTTTTG ACACTACATT CCAAAGTCAC CTGAACAAAG CCTTATGGAA GGTAGGGAAG 
TTAACTAGTC CTGGGGCTTT GCTCTTTCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001202.4
CDS	213..3365
Translation	MAAVKKEGGALSEAMSLEGDEWELSKENVQPLRQGRIMSTLQGALAQESACNNTLQQQKRAFEYEIRFYTGNDPLDVWDRYISWTEQNYPQGGKESNMSTLLERAVEALQGEKRYYSDPRFLNLWLKLGRLCNEPLDMYSYLHNQGIGVSLAQFYISWAEEYEARENFRKADAIFQEGIQQKAEPLERLQSQHRQFQARVSRQTLLALEKEEEEEVFESSVPQRSTLAELKSKGKKTARAPIIRVGGALKAPSQNRGLQNPFPQQMQNNSRITVFDENADEASTAELSKPTVQPWIAPPMPRAKENELQAGPWNTGRSLEHRPRGNTASLIAVPAVLPSFTPYVEETAQQPVMTPCKIEPSINHILSTRKPGKEEGDPLQRVQSHQQASEEKKEKMMYCKEKIYAGVGEFSFEEIRAEVFRKKLKEQREAELLTSAEKRAEMQKQIEEMEKKLKEIQTTQQERTGDQQEETMPTKETTKLQIASESQKIPGMTLSSSVCQVNCCARETSLAENIWQEQPHSKGPSVPFSIFDEFLLSEKKNKSPPADPPRVLAQRRPLAVLKTSESITSNEDVSPDVCDEFTGIEPLSEDAIITGFRNVTICPNPEDTCDFARAARFVSTPFHEIMSLKDLPSDPERLLPEEDLDVKTSEDQQTACGTIYSQTLSIKKLSPIIEDSREATHSSGFSGSSASVASTSSIKCLQIPEKLELTNETSENPTQSPWCSQYRRQLLKSLPELSASAELCIEDRPMPKLEIEKEIELGNEDYCIKREYLICEDYKLFWVAPRNSAELTVIKVSSQPVPWDFYINLKLKERLNEDFDHFCSCYQYQDGCIVWHQYINCFTLQDLLQHSEYITHEITVLIIYNLLTIVEMLHKAEIVHGDLSPRCLILRNRIHDPYDCNKNNQALKIVDFSYSVDLRVQLDVFTLSGFRTVQILEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQVFWDGSFWKLSQNISELKDGELWNKFFVRILNANDEATVSVLGELAAEMNGVFDTTFQSHLNKALWKVGKLTSPGALLFQ

Target ORF information:

RefSeq Version	NM_001211.5
Organism	Homo sapiens(human)
Definition	Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001211.5

ORF Insert Sequence:

ATGGCGGCGG TGAAGAAGGA AGGGGGTGCT CTGAGTGAAG CCATGTCCCT GGAGGGAGAT 
GAATGGGAAC TGAGTAAAGA AAATGTACAA CCTTTAAGGC AAGGGCGGAT CATGTCCACG 
CTTCAGGGAG CACTGGCACA AGAATCTGCC TGTAACAATA CTCTTCAGCA GCAGAAACGG 
GCATTTGAAT ATGAAATTCG ATTTTACACT GGAAATGACC CTCTGGATGT TTGGGATAGG 
TATATCAGCT GGACAGAGCA GAACTATCCT CAAGGTGGGA AGGAGAGTAA TATGTCAACG 
TTATTAGAAA GAGCTGTAGA AGCACTACAA GGAGAAAAAC GATATTATAG TGATCCTCGA 
TTTCTCAATC TCTGGCTTAA ATTAGGGCGT TTATGCAATG AGCCTTTGGA TATGTACAGT 
TACTTGCACA ACCAAGGGAT TGGTGTTTCA CTTGCTCAGT TCTATATCTC ATGGGCAGAA 
GAATATGAAG CTAGAGAAAA CTTTAGGAAA GCAGATGCGA TATTTCAGGA AGGGATTCAA 
CAGAAGGCTG AACCACTAGA AAGACTACAG TCCCAGCACC GACAATTCCA AGCTCGAGTG 
TCTCGGCAAA CTCTGTTGGC ACTTGAGAAA GAAGAAGAGG AGGAAGTTTT TGAGTCTTCT 
GTACCACAAC GAAGCACACT AGCTGAACTA AAGAGCAAAG GGAAAAAGAC AGCAAGAGCT 
CCAATCATCC GTGTAGGAGG TGCTCTCAAG GCTCCAAGCC AGAACAGAGG ACTCCAAAAT 
CCATTTCCTC AACAGATGCA AAATAATAGT AGAATTACTG TTTTTGATGA AAATGCTGAT 
GAGGCTTCTA CAGCAGAGTT GTCTAAGCCT ACAGTCCAGC CATGGATAGC ACCCCCCATG 
CCCAGGGCCA AAGAGAATGA GCTGCAAGCA GGCCCTTGGA ACACAGGCAG GTCCTTGGAA 
CACAGGCCTC GTGGCAATAC AGCTTCACTG ATAGCTGTAC CCGCTGTGCT TCCCAGTTTC 
ACTCCATATG TGGAAGAGAC TGCACAACAG CCAGTTATGA CACCATGTAA AATTGAACCT 
AGTATAAACC ACATCCTAAG CACCAGAAAG CCTGGAAAGG AAGAAGGAGA TCCTCTACAA 
AGGGTTCAGA GCCATCAGCA AGCGTCTGAG GAGAAGAAAG AGAAGATGAT GTATTGTAAG 
GAGAAGATTT ATGCAGGAGT AGGGGAATTC TCCTTTGAAG AAATTCGGGC TGAAGTTTTC 
CGGAAGAAAT TAAAAGAGCA AAGGGAAGCC GAGCTATTGA CCAGTGCAGA GAAGAGAGCA 
GAAATGCAGA AACAGATTGA AGAGATGGAG AAGAAGCTAA AAGAAATCCA AACTACTCAG 
CAAGAAAGAA CAGGTGATCA GCAAGAAGAG ACGATGCCTA CAAAGGAGAC AACTAAACTG 
CAAATTGCTT CCGAGTCTCA GAAAATACCA GGAATGACTC TATCCAGTTC TGTTTGTCAA 
GTAAACTGTT GTGCCAGAGA AACTTCACTT GCGGAGAACA TTTGGCAGGA ACAACCTCAT 
TCTAAAGGTC CCAGTGTACC TTTCTCCATT TTTGATGAGT TTCTTCTTTC AGAAAAGAAG 
AATAAAAGTC CTCCTGCAGA TCCCCCACGA GTTTTAGCTC AACGAAGACC CCTTGCAGTT 
CTCAAAACCT CAGAAAGCAT CACCTCAAAT GAAGATGTGT CTCCAGATGT TTGTGATGAA 
TTTACAGGAA TTGAACCCTT GAGCGAGGAT GCCATTATCA CAGGCTTCAG AAATGTAACA 
ATTTGTCCTA ACCCAGAAGA CACTTGTGAC TTTGCCAGAG CAGCTCGTTT TGTATCCACT 
CCTTTTCATG AGATAATGTC CTTGAAGGAT CTCCCTTCTG ATCCTGAGAG ACTGTTACCG 
GAAGAAGATC TAGATGTAAA GACCTCTGAG GACCAGCAGA CAGCTTGTGG CACTATCTAC 
AGTCAGACTC TCAGCATCAA GAAGCTGAGC CCAATTATTG AAGACAGTCG TGAAGCCACA 
CACTCCTCTG GCTTCTCTGG TTCTTCTGCC TCGGTTGCAA GCACCTCCTC CATCAAATGT 
CTTCAAATTC CTGAGAAACT AGAACTTACT AATGAGACTT CAGAAAACCC TACTCAGTCA 
CCATGGTGTT CACAGTATCG CAGACAGCTA CTGAAGTCCC TACCAGAGTT AAGTGCCTCT 
GCAGAGTTGT GTATAGAAGA CAGACCAATG CCTAAGTTGG AAATTGAGAA GGAAATTGAA 
TTAGGTAATG AGGATTACTG CATTAAACGA GAATACCTAA TATGTGAAGA TTACAAGTTA 
TTCTGGGTGG CGCCAAGAAA CTCTGCAGAA TTAACAGTAA TAAAGGTATC TTCTCAACCT 
GTCCCATGGG ACTTTTATAT CAACCTCAAG TTAAAGGAAC GTTTAAATGA AGATTTTGAT 
CATTTTTGCA GCTGTTATCA ATATCAAGAT GGCTGTATTG TTTGGCACCA ATATATAAAC 
TGCTTCACCC TTCAGGATCT TCTCCAACAC AGTGAATATA TTACCCATGA AATAACAGTG 
TTGATTATTT ATAACCTTTT GACAATAGTG GAGATGCTAC ACAAAGCAGA AATAGTCCAT 
GGTGACTTGA GTCCAAGGTG TCTGATTCTC AGAAACAGAA TCCACGATCC CTATGATTGT 
AACAAGAACA ATCAAGCTTT GAAGATAGTG GACTTTTCCT ACAGTGTTGA CCTTAGGGTG 
CAGCTGGATG TTTTTACCCT CAGCGGCTTT CGGACTGTAC AGATCCTGGA AGGACAAAAG 
ATCCTGGCTA ACTGTTCTTC TCCCTACCAG GTAGACCTGT TTGGTATAGC AGATTTAGCA 
CATTTACTAT TGTTCAAGGA ACACCTACAG GTCTTCTGGG ATGGGTCCTT CTGGAAACTT 
AGCCAAAATA TTTCTGAGCT AAAAGATGGT GAATTGTGGA ATAAATTCTT TGTGCGGATT 
CTGAATGCCA ATGATGAGGC CACAGTGTCT GTTCTTGGGG AGCTTGCAGC AGAAATGAAT 
GGGGTTTTTG ACACTACATT CCAAAGTCAC CTGAACAAAG CCTTATGGAA GGTAGGGAAG 
TTAACTAGTC CTGGGGCTTT GCTCTTTCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BUB1B ( NM_001211.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_001202.5 Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), mRNA.

Price & Availability↑

CloneID	OHu109353
Clone ID Related Accession (Same CDS sequence)	NM_001211.6
Accession Version	NM_001211.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 beta
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025429.6. On Nov 30, 2019 this sequence version replaced NM_001211.5. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF107297.1, AF068760.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG TGAAGAAGGA AGGGGGTGCT CTGAGTGAAG CCATGTCCCT GGAGGGAGAT 
GAATGGGAAC TGAGTAAAGA AAATGTACAA CCTTTAAGGC AAGGGCGGAT CATGTCCACG 
CTTCAGGGAG CACTGGCACA AGAATCTGCC TGTAACAATA CTCTTCAGCA GCAGAAACGG 
GCATTTGAAT ATGAAATTCG ATTTTACACT GGAAATGACC CTCTGGATGT TTGGGATAGG 
TATATCAGCT GGACAGAGCA GAACTATCCT CAAGGTGGGA AGGAGAGTAA TATGTCAACG 
TTATTAGAAA GAGCTGTAGA AGCACTACAA GGAGAAAAAC GATATTATAG TGATCCTCGA 
TTTCTCAATC TCTGGCTTAA ATTAGGGCGT TTATGCAATG AGCCTTTGGA TATGTACAGT 
TACTTGCACA ACCAAGGGAT TGGTGTTTCA CTTGCTCAGT TCTATATCTC ATGGGCAGAA 
GAATATGAAG CTAGAGAAAA CTTTAGGAAA GCAGATGCGA TATTTCAGGA AGGGATTCAA 
CAGAAGGCTG AACCACTAGA AAGACTACAG TCCCAGCACC GACAATTCCA AGCTCGAGTG 
TCTCGGCAAA CTCTGTTGGC ACTTGAGAAA GAAGAAGAGG AGGAAGTTTT TGAGTCTTCT 
GTACCACAAC GAAGCACACT AGCTGAACTA AAGAGCAAAG GGAAAAAGAC AGCAAGAGCT 
CCAATCATCC GTGTAGGAGG TGCTCTCAAG GCTCCAAGCC AGAACAGAGG ACTCCAAAAT 
CCATTTCCTC AACAGATGCA AAATAATAGT AGAATTACTG TTTTTGATGA AAATGCTGAT 
GAGGCTTCTA CAGCAGAGTT GTCTAAGCCT ACAGTCCAGC CATGGATAGC ACCCCCCATG 
CCCAGGGCCA AAGAGAATGA GCTGCAAGCA GGCCCTTGGA ACACAGGCAG GTCCTTGGAA 
CACAGGCCTC GTGGCAATAC AGCTTCACTG ATAGCTGTAC CCGCTGTGCT TCCCAGTTTC 
ACTCCATATG TGGAAGAGAC TGCACGACAG CCAGTTATGA CACCATGTAA AATTGAACCT 
AGTATAAACC ACATCCTAAG CACCAGAAAG CCTGGAAAGG AAGAAGGAGA TCCTCTACAA 
AGGGTTCAGA GCCATCAGCA AGCGTCTGAG GAGAAGAAAG AGAAGATGAT GTATTGTAAG 
GAGAAGATTT ATGCAGGAGT AGGGGAATTC TCCTTTGAAG AAATTCGGGC TGAAGTTTTC 
CGGAAGAAAT TAAAAGAGCA AAGGGAAGCC GAGCTATTGA CCAGTGCAGA GAAGAGAGCA 
GAAATGCAGA AACAGATTGA AGAGATGGAG AAGAAGCTAA AAGAAATCCA AACTACTCAG 
CAAGAAAGAA CAGGTGATCA GCAAGAAGAG ACGATGCCTA CAAAGGAGAC AACTAAACTG 
CAAATTGCTT CCGAGTCTCA GAAAATACCA GGAATGACTC TATCCAGTTC TGTTTGTCAA 
GTAAACTGTT GTGCCAGAGA AACTTCACTT GCGGAGAACA TTTGGCAGGA ACAACCTCAT 
TCTAAAGGTC CCAGTGTACC TTTCTCCATT TTTGATGAGT TTCTTCTTTC AGAAAAGAAG 
AATAAAAGTC CTCCTGCAGA TCCCCCACGA GTTTTAGCTC AACGAAGACC CCTTGCAGTT 
CTCAAAACCT CAGAAAGCAT CACCTCAAAT GAAGATGTGT CTCCAGATGT TTGTGATGAA 
TTTACAGGAA TTGAACCCTT GAGCGAGGAT GCCATTATCA CAGGCTTCAG AAATGTAACA 
ATTTGTCCTA ACCCAGAAGA CACTTGTGAC TTTGCCAGAG CAGCTCGTTT TGTATCCACT 
CCTTTTCATG AGATAATGTC CTTGAAGGAT CTCCCTTCTG ATCCTGAGAG ACTGTTACCG 
GAAGAAGATC TAGATGTAAA GACCTCTGAG GACCAGCAGA CAGCTTGTGG CACTATCTAC 
AGTCAGACTC TCAGCATCAA GAAGCTGAGC CCAATTATTG AAGACAGTCG TGAAGCCACA 
CACTCCTCTG GCTTCTCTGG TTCTTCTGCC TCGGTTGCAA GCACCTCCTC CATCAAATGT 
CTTCAAATTC CTGAGAAACT AGAACTTACT AATGAGACTT CAGAAAACCC TACTCAGTCA 
CCATGGTGTT CACAGTATCG CAGACAGCTA CTGAAGTCCC TACCAGAGTT AAGTGCCTCT 
GCAGAGTTGT GTATAGAAGA CAGACCAATG CCTAAGTTGG AAATTGAGAA GGAAATTGAA 
TTAGGTAATG AGGATTACTG CATTAAACGA GAATACCTAA TATGTGAAGA TTACAAGTTA 
TTCTGGGTGG CGCCAAGAAA CTCTGCAGAA TTAACAGTAA TAAAGGTATC TTCTCAACCT 
GTCCCATGGG ACTTTTATAT CAACCTCAAG TTAAAGGAAC GTTTAAATGA AGATTTTGAT 
CATTTTTGCA GCTGTTATCA ATATCAAGAT GGCTGTATTG TTTGGCACCA ATATATAAAC 
TGCTTCACCC TTCAGGATCT TCTCCAACAC AGTGAATATA TTACCCATGA AATAACAGTG 
TTGATTATTT ATAACCTTTT GACAATAGTG GAGATGCTAC ACAAAGCAGA AATAGTCCAT 
GGTGACTTGA GTCCAAGGTG TCTGATTCTC AGAAACAGAA TCCACGATCC CTATGATTGT 
AACAAGAACA ATCAAGCTTT GAAGATAGTG GACTTTTCCT ACAGTGTTGA CCTTAGGGTG 
CAGCTGGATG TTTTTACCCT CAGCGGCTTT CGGACTGTAC AGATCCTGGA AGGACAAAAG 
ATCCTGGCTA ACTGTTCTTC TCCCTACCAG GTAGACCTGT TTGGTATAGC AGATTTAGCA 
CATTTACTAT TGTTCAAGGA ACACCTACAG GTCTTCTGGG ATGGGTCCTT CTGGAAACTT 
AGCCAAAATA TTTCTGAGCT AAAAGATGGT GAATTGTGGA ATAAATTCTT TGTGCGGATT 
CTGAATGCCA ATGATGAGGC CACAGTGTCT GTTCTTGGGG AGCTTGCAGC AGAAATGAAT 
GGGGTTTTTG ACACTACATT CCAAAGTCAC CTGAACAAAG CCTTATGGAA GGTAGGGAAG 
TTAACTAGTC CTGGGGCTTT GCTCTTTCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001202.5
CDS	153..3305
Translation	MAAVKKEGGALSEAMSLEGDEWELSKENVQPLRQGRIMSTLQGALAQESACNNTLQQQKRAFEYEIRFYTGNDPLDVWDRYISWTEQNYPQGGKESNMSTLLERAVEALQGEKRYYSDPRFLNLWLKLGRLCNEPLDMYSYLHNQGIGVSLAQFYISWAEEYEARENFRKADAIFQEGIQQKAEPLERLQSQHRQFQARVSRQTLLALEKEEEEEVFESSVPQRSTLAELKSKGKKTARAPIIRVGGALKAPSQNRGLQNPFPQQMQNNSRITVFDENADEASTAELSKPTVQPWIAPPMPRAKENELQAGPWNTGRSLEHRPRGNTASLIAVPAVLPSFTPYVEETARQPVMTPCKIEPSINHILSTRKPGKEEGDPLQRVQSHQQASEEKKEKMMYCKEKIYAGVGEFSFEEIRAEVFRKKLKEQREAELLTSAEKRAEMQKQIEEMEKKLKEIQTTQQERTGDQQEETMPTKETTKLQIASESQKIPGMTLSSSVCQVNCCARETSLAENIWQEQPHSKGPSVPFSIFDEFLLSEKKNKSPPADPPRVLAQRRPLAVLKTSESITSNEDVSPDVCDEFTGIEPLSEDAIITGFRNVTICPNPEDTCDFARAARFVSTPFHEIMSLKDLPSDPERLLPEEDLDVKTSEDQQTACGTIYSQTLSIKKLSPIIEDSREATHSSGFSGSSASVASTSSIKCLQIPEKLELTNETSENPTQSPWCSQYRRQLLKSLPELSASAELCIEDRPMPKLEIEKEIELGNEDYCIKREYLICEDYKLFWVAPRNSAELTVIKVSSQPVPWDFYINLKLKERLNEDFDHFCSCYQYQDGCIVWHQYINCFTLQDLLQHSEYITHEITVLIIYNLLTIVEMLHKAEIVHGDLSPRCLILRNRIHDPYDCNKNNQALKIVDFSYSVDLRVQLDVFTLSGFRTVQILEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQVFWDGSFWKLSQNISELKDGELWNKFFVRILNANDEATVSVLGELAAEMNGVFDTTFQSHLNKALWKVGKLTSPGALLFQ

Target ORF information:

RefSeq Version	NM_001211.6
Organism	Homo sapiens(human)
Definition	Homo sapiens BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001211.6

ORF Insert Sequence:

ATGGCGGCGG TGAAGAAGGA AGGGGGTGCT CTGAGTGAAG CCATGTCCCT GGAGGGAGAT 
GAATGGGAAC TGAGTAAAGA AAATGTACAA CCTTTAAGGC AAGGGCGGAT CATGTCCACG 
CTTCAGGGAG CACTGGCACA AGAATCTGCC TGTAACAATA CTCTTCAGCA GCAGAAACGG 
GCATTTGAAT ATGAAATTCG ATTTTACACT GGAAATGACC CTCTGGATGT TTGGGATAGG 
TATATCAGCT GGACAGAGCA GAACTATCCT CAAGGTGGGA AGGAGAGTAA TATGTCAACG 
TTATTAGAAA GAGCTGTAGA AGCACTACAA GGAGAAAAAC GATATTATAG TGATCCTCGA 
TTTCTCAATC TCTGGCTTAA ATTAGGGCGT TTATGCAATG AGCCTTTGGA TATGTACAGT 
TACTTGCACA ACCAAGGGAT TGGTGTTTCA CTTGCTCAGT TCTATATCTC ATGGGCAGAA 
GAATATGAAG CTAGAGAAAA CTTTAGGAAA GCAGATGCGA TATTTCAGGA AGGGATTCAA 
CAGAAGGCTG AACCACTAGA AAGACTACAG TCCCAGCACC GACAATTCCA AGCTCGAGTG 
TCTCGGCAAA CTCTGTTGGC ACTTGAGAAA GAAGAAGAGG AGGAAGTTTT TGAGTCTTCT 
GTACCACAAC GAAGCACACT AGCTGAACTA AAGAGCAAAG GGAAAAAGAC AGCAAGAGCT 
CCAATCATCC GTGTAGGAGG TGCTCTCAAG GCTCCAAGCC AGAACAGAGG ACTCCAAAAT 
CCATTTCCTC AACAGATGCA AAATAATAGT AGAATTACTG TTTTTGATGA AAATGCTGAT 
GAGGCTTCTA CAGCAGAGTT GTCTAAGCCT ACAGTCCAGC CATGGATAGC ACCCCCCATG 
CCCAGGGCCA AAGAGAATGA GCTGCAAGCA GGCCCTTGGA ACACAGGCAG GTCCTTGGAA 
CACAGGCCTC GTGGCAATAC AGCTTCACTG ATAGCTGTAC CCGCTGTGCT TCCCAGTTTC 
ACTCCATATG TGGAAGAGAC TGCACGACAG CCAGTTATGA CACCATGTAA AATTGAACCT 
AGTATAAACC ACATCCTAAG CACCAGAAAG CCTGGAAAGG AAGAAGGAGA TCCTCTACAA 
AGGGTTCAGA GCCATCAGCA AGCGTCTGAG GAGAAGAAAG AGAAGATGAT GTATTGTAAG 
GAGAAGATTT ATGCAGGAGT AGGGGAATTC TCCTTTGAAG AAATTCGGGC TGAAGTTTTC 
CGGAAGAAAT TAAAAGAGCA AAGGGAAGCC GAGCTATTGA CCAGTGCAGA GAAGAGAGCA 
GAAATGCAGA AACAGATTGA AGAGATGGAG AAGAAGCTAA AAGAAATCCA AACTACTCAG 
CAAGAAAGAA CAGGTGATCA GCAAGAAGAG ACGATGCCTA CAAAGGAGAC AACTAAACTG 
CAAATTGCTT CCGAGTCTCA GAAAATACCA GGAATGACTC TATCCAGTTC TGTTTGTCAA 
GTAAACTGTT GTGCCAGAGA AACTTCACTT GCGGAGAACA TTTGGCAGGA ACAACCTCAT 
TCTAAAGGTC CCAGTGTACC TTTCTCCATT TTTGATGAGT TTCTTCTTTC AGAAAAGAAG 
AATAAAAGTC CTCCTGCAGA TCCCCCACGA GTTTTAGCTC AACGAAGACC CCTTGCAGTT 
CTCAAAACCT CAGAAAGCAT CACCTCAAAT GAAGATGTGT CTCCAGATGT TTGTGATGAA 
TTTACAGGAA TTGAACCCTT GAGCGAGGAT GCCATTATCA CAGGCTTCAG AAATGTAACA 
ATTTGTCCTA ACCCAGAAGA CACTTGTGAC TTTGCCAGAG CAGCTCGTTT TGTATCCACT 
CCTTTTCATG AGATAATGTC CTTGAAGGAT CTCCCTTCTG ATCCTGAGAG ACTGTTACCG 
GAAGAAGATC TAGATGTAAA GACCTCTGAG GACCAGCAGA CAGCTTGTGG CACTATCTAC 
AGTCAGACTC TCAGCATCAA GAAGCTGAGC CCAATTATTG AAGACAGTCG TGAAGCCACA 
CACTCCTCTG GCTTCTCTGG TTCTTCTGCC TCGGTTGCAA GCACCTCCTC CATCAAATGT 
CTTCAAATTC CTGAGAAACT AGAACTTACT AATGAGACTT CAGAAAACCC TACTCAGTCA 
CCATGGTGTT CACAGTATCG CAGACAGCTA CTGAAGTCCC TACCAGAGTT AAGTGCCTCT 
GCAGAGTTGT GTATAGAAGA CAGACCAATG CCTAAGTTGG AAATTGAGAA GGAAATTGAA 
TTAGGTAATG AGGATTACTG CATTAAACGA GAATACCTAA TATGTGAAGA TTACAAGTTA 
TTCTGGGTGG CGCCAAGAAA CTCTGCAGAA TTAACAGTAA TAAAGGTATC TTCTCAACCT 
GTCCCATGGG ACTTTTATAT CAACCTCAAG TTAAAGGAAC GTTTAAATGA AGATTTTGAT 
CATTTTTGCA GCTGTTATCA ATATCAAGAT GGCTGTATTG TTTGGCACCA ATATATAAAC 
TGCTTCACCC TTCAGGATCT TCTCCAACAC AGTGAATATA TTACCCATGA AATAACAGTG 
TTGATTATTT ATAACCTTTT GACAATAGTG GAGATGCTAC ACAAAGCAGA AATAGTCCAT 
GGTGACTTGA GTCCAAGGTG TCTGATTCTC AGAAACAGAA TCCACGATCC CTATGATTGT 
AACAAGAACA ATCAAGCTTT GAAGATAGTG GACTTTTCCT ACAGTGTTGA CCTTAGGGTG 
CAGCTGGATG TTTTTACCCT CAGCGGCTTT CGGACTGTAC AGATCCTGGA AGGACAAAAG 
ATCCTGGCTA ACTGTTCTTC TCCCTACCAG GTAGACCTGT TTGGTATAGC AGATTTAGCA 
CATTTACTAT TGTTCAAGGA ACACCTACAG GTCTTCTGGG ATGGGTCCTT CTGGAAACTT 
AGCCAAAATA TTTCTGAGCT AAAAGATGGT GAATTGTGGA ATAAATTCTT TGTGCGGATT 
CTGAATGCCA ATGATGAGGC CACAGTGTCT GTTCTTGGGG AGCTTGCAGC AGAAATGAAT 
GGGGTTTTTG ACACTACATT CCAAAGTCAC CTGAACAAAG CCTTATGGAA GGTAGGGAAG 
TTAACTAGTC CTGGGGCTTT GCTCTTTCAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The FOXM1/BUB1B signaling pathway is essential for the tumorigenicity and radioresistance of glioblastoma.
Oncology reports38(6)3367-3375(2017 Dec)
Ma Q,Liu Y,Shang L,Yu J,Qu Q

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BUB1B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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