Bmp15 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bmp15
Entrez Gene ID	12155
Full Name	bone morphogenetic protein 15
Synonyms	AU015375,AU018861,AU021453,Bmp-15,C86824,C87336,GDF-9B
General protein information	Preferred Names bone morphogenetic protein 15 Names bone morphogenetic protein 15 growth/differentiation factor 9B
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: X Map Location: X A1.1\|X 2.81 cM
Summary	This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate subunits of a disulfide-linked homodimer, or alternatively, a heterodimer, with the related protein, growth differentiation factor 9 (GDF9). This protein plays a role in oocyte maturation and follicular development, through activation of granulosa cells. Female mice lacking a functional copy of this gene exhibit impaired fertility. [provided by RefSeq, Aug 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_009757.5	NP_033887.1	bone morphogenetic protein 15 preproprotein

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Bmp15	NP_033887.1
Bos taurus (cattle)	BMP15	NP_001026922.1
Xenopus tropicalis (tropical clawed frog)	LOC100485893	XP_002936834.2
Homo sapiens (human)	BMP15	NP_005439.2
Pan troglodytes (chimpanzee)	BMP15	XP_529247.2
	BMP15	XP_001083980.1
Rattus norvegicus (Norway rat)	Bmp15	NP_067702.1
Gallus gallus (chicken)	BMP15	NP_001006589.2
Canis lupus familiaris (dog)	BMP15	XP_003640322.1
Danio rerio (zebrafish)	bmp15	NP_001018320.1

Gene Ontology
Bibliography

Related articles in PubMed

Rac1 modulates the formation of primordial follicles by facilitating STAT3-directed Jagged1, GDF9 and BMP15 transcription in mice.
Zhao L, Du X, Huang K, Zhang T, Teng Z, Niu W, Wang C, Xia G
Scientific reports623972(2016 Apr)

Antagonist Xist and Tsix co-transcription during mouse oogenesis and maternal Xist expression during pre-implantation development calls into question the nature of the maternal imprint on the X chromosome.
Deuve JL, Bonnet-Garnier A, Beaujean N, Avner P, Morey C
Epigenetics10(10)931-42(2015)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that Rac1 induced nuclear import of STAT3 by physical binding, and nuclear STAT3 directly activated the transcription of essential oocyte-specific genes, including Jagged1, GDF9 and BMP15.
Title: Rac1 modulates the formation of primordial follicles by facilitating STAT3-directed Jagged1, GDF9 and BMP15 transcription in mice.

results support a model of activation for human GDF9 dependent on cumulin formation through heterodimerization with BMP15. Oocyte-secreted cumulin is likely to be a central regulator of fertility in mono-ovular mammals.
Title: Cumulin, an Oocyte-secreted Heterodimer of the Transforming Growth Factor-? Family, Is a Potent Activator of Granulosa Cells and Improves Oocyte Quality.

these data provide evidence that the preovulatory LH surge leads to upregulation of several forms of BMP15 protein secreted by the oocyte for putative sequestration and/or interaction with ovarian follicular somatic cells.
Title: Oocyte expression, secretion and somatic cell interaction of mouse bone morphogenetic protein 15 during the peri-ovulatory period.

modified oocyte glycoproteins alter GDF9:BMP15 expression modifying follicle development resulting in the generation of more follicles
Title: Oocytes lacking O-glycans alter follicle development and increase fertility by increasing follicle FSH sensitivity, decreasing apoptosis, and modifying GDF9:BMP15 expression.

findings that GDF9:BMP15 heterodimers are the most bioactive ligands in mice and humans compared with homodimers explain many puzzling genetic and physiological data and have important implications for improving female fertility in mammals
Title: Growth differentiation factor 9:bone morphogenetic protein 15 heterodimers are potent regulators of ovarian functions.

The following Bmp15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bmp15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu22184	NM_009757.5 Latest version!	Mus musculus bone morphogenetic protein 15 (Bmp15), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bmp15 ( NM_009757.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033887.1 Mus musculus bone morphogenetic protein 15 (Bmp15), mRNA.

Price & Availability↑

CloneID	OMu22184
Clone ID Related Accession (Same CDS sequence)	NM_009757.5
Accession Version	NM_009757.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Mus musculus(house mouse)
Product	bone morphogenetic protein 15 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK135882.1, AK139773.1 and AU019682.1. On Aug 30, 2016 this sequence version replaced NM_009757.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK139847.1, BC055363.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCTTC TCACAATTCT TAGAATTCTT TTGTGGGGAG TGGTGCTTTT TATGGAACAG 
AGGGTCCAAA TGGCAAAGCC AGGGTGGCCC TCCACCGCCC TCCTTGCTGA CGACCCTACA 
TTGCCCTCAA TTTTGGATCT GGCCAAAGAA GCCCCTGGCA AGGAGATGAA GCAATGGCCC 
CAAGGCTATC CCCTGAGGTA CATGCTCAAG TTATACCATC GTTCGGCTGA CCCGCATGGC 
CATCCAAGGG AGAACCGCAC GATTGGAGCG AAAATGGTGA GGCTGGTAAA GCCGTCGGCC 
AACACAGTAA GGCCTCCCAG AGGTTCCTGG CATGTACAGA CCCTGGACTT TCCTCTAGCA 
TCAAACCAGG TAGCATACGA ACTAATCAGA GCCACTGTGG TTTACCGCCA TCAACTTCAT 
CTAGTTAATT ACCATCTCTC CTGCCATGTG GAAACTTGGG TTCCTAAATG CCGGACCAAG 
CACTTACCTT CTTCTAAATC GGGTTCCTCA AAGCCTTCTC CCATGTCTAA AGCCTGGACA 
GAGATAGATA TTACACATTG TATTCAGCAG AAGCTCTGGA ATCGCAAGGG ACGGAGTGTT 
CTTCGCCTCC GCTTCATGTG TCAGCAGCAA AAAGGCAATG AGACTCGTGA GTTCCGGTGG 
CATGGCATGA CATCCTTGGA TGTTGCCTTC TTGCTACTCT ATTTCAATGA CACCGATGAC 
AGAGTTCAGG GTAAACTTCT TGCAAGAGGC CAAGAGGAGT TAACTGATAG GGAATCTTCT 
TTTCTCATGC GGAGTGTCCG GCAAGCATGC AGCATTGAAT CTGATGCCTC TTGTCCTTCT 
CAGGAACATG ATGGGTCTGT AAATAACCAG TGTTCCCTCC ATCCTTACAA GGTCAGCTTC 
CACCAACTAG GCTGGGATCA CTGGATCATT GCTCCTCGTC TCTATACCCC AAATTACTGT 
AAAGGAATCT GTACTCGGGT ATTACCCTAT GGTCTCAATT CACCCAACCA TGCCATCATT 
CAGAGCCTTG TCAATGAACT AGTGAATCAC AGTGTACCTC AGCCTTCCTG TGTCCCTTAT 
AATTTTCTTC CTATGAGCAT CCTCCTGATT GAGACCAACG GGAGTATCTT GTACAAGGAG 
TATGAGGGTA TGATTGCCCA GTCCTGTACA TGTAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033887.1
CDS	362..1540
Translation	MALLTILRILLWGVVLFMEQRVQMAKPGWPSTALLADDPTLPSILDLAKEAPGKEMKQWPQGYPLRYMLKLYHRSADPHGHPRENRTIGAKMVRLVKPSANTVRPPRGSWHVQTLDFPLASNQVAYELIRATVVYRHQLHLVNYHLSCHVETWVPKCRTKHLPSSKSGSSKPSPMSKAWTEIDITHCIQQKLWNRKGRSVLRLRFMCQQQKGNETREFRWHGMTSLDVAFLLLYFNDTDDRVQGKLLARGQEELTDRESSFLMRSVRQACSIESDASCPSQEHDGSVNNQCSLHPYKVSFHQLGWDHWIIAPRLYTPNYCKGICTRVLPYGLNSPNHAIIQSLVNELVNHSVPQPSCVPYNFLPMSILLIETNGSILYKEYEGMIAQSCTCR

Target ORF information:

RefSeq Version	NM_009757.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus bone morphogenetic protein 15 (Bmp15), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009757.5

ORF Insert Sequence:

ATGGCCCTTC TCACAATTCT TAGAATTCTT TTGTGGGGAG TGGTGCTTTT TATGGAACAG 
AGGGTCCAAA TGGCAAAGCC AGGGTGGCCC TCCACCGCCC TCCTTGCTGA CGACCCTACA 
TTGCCCTCAA TTTTGGATCT GGCCAAAGAA GCCCCTGGCA AGGAGATGAA GCAATGGCCC 
CAAGGCTATC CCCTGAGGTA CATGCTCAAG TTATACCATC GTTCGGCTGA CCCGCATGGC 
CATCCAAGGG AGAACCGCAC GATTGGAGCG AAAATGGTGA GGCTGGTAAA GCCGTCGGCC 
AACACAGTAA GGCCTCCCAG AGGTTCCTGG CATGTACAGA CCCTGGACTT TCCTCTAGCA 
TCAAACCAGG TAGCATACGA ACTAATCAGA GCCACTGTGG TTTACCGCCA TCAACTTCAT 
CTAGTTAATT ACCATCTCTC CTGCCATGTG GAAACTTGGG TTCCTAAATG CCGGACCAAG 
CACTTACCTT CTTCTAAATC GGGTTCCTCA AAGCCTTCTC CCATGTCTAA AGCCTGGACA 
GAGATAGATA TTACACATTG TATTCAGCAG AAGCTCTGGA ATCGCAAGGG ACGGAGTGTT 
CTTCGCCTCC GCTTCATGTG TCAGCAGCAA AAAGGCAATG AGACTCGTGA GTTCCGGTGG 
CATGGCATGA CATCCTTGGA TGTTGCCTTC TTGCTACTCT ATTTCAATGA CACCGATGAC 
AGAGTTCAGG GTAAACTTCT TGCAAGAGGC CAAGAGGAGT TAACTGATAG GGAATCTTCT 
TTTCTCATGC GGAGTGTCCG GCAAGCATGC AGCATTGAAT CTGATGCCTC TTGTCCTTCT 
CAGGAACATG ATGGGTCTGT AAATAACCAG TGTTCCCTCC ATCCTTACAA GGTCAGCTTC 
CACCAACTAG GCTGGGATCA CTGGATCATT GCTCCTCGTC TCTATACCCC AAATTACTGT 
AAAGGAATCT GTACTCGGGT ATTACCCTAT GGTCTCAATT CACCCAACCA TGCCATCATT 
CAGAGCCTTG TCAATGAACT AGTGAATCAC AGTGTACCTC AGCCTTCCTG TGTCCCTTAT 
AATTTTCTTC CTATGAGCAT CCTCCTGATT GAGACCAACG GGAGTATCTT GTACAAGGAG 
TATGAGGGTA TGATTGCCCA GTCCTGTACA TGTAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Rac1 modulates the formation of primordial follicles by facilitating STAT3-directed Jagged1, GDF9 and BMP15 transcription in mice.
Scientific reports623972(2016 Apr)
Zhao L,Du X,Huang K,Zhang T,Teng Z,Niu W,Wang C,Xia G

Antagonist Xist and Tsix co-transcription during mouse oogenesis and maternal Xist expression during pre-implantation development calls into question the nature of the maternal imprint on the X chromosome.
Epigenetics10(10)931-42(2015)
Deuve JL,Bonnet-Garnier A,Beaujean N,Avner P,Morey C

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Bmp15 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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