gRNAs
The following Acan gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Acan cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OMu17926 | |
| Clone ID Related Accession (Same CDS sequence) | NM_007424.2 , NM_007424.3 | |
| Accession Version | NM_007424.2 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 6399bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-12-30 | |
| Organism | Mus musculus(house mouse) | |
| Product | aggrecan core protein precursor | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC110520.12. On Oct 31, 2006 this sequence version replaced NM_007424.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGACCACTT TACTCTTGGT CTTTGTGACT CTGAGGGTCA TCGCTGCAGT GATCTCAGAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_031450.2 |
| CDS | 134..6532 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_007424.2 |
1 | ATGACCACTT TACTCTTGGT CTTTGTGACT CTGAGGGTCA TCGCTGCAGT GATCTCAGAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu17926 | |
| Clone ID Related Accession (Same CDS sequence) | NM_007424.2 , NM_007424.3 | |
| Accession Version | NM_007424.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 6399bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-30 | |
| Organism | Mus musculus(house mouse) | |
| Product | aggrecan core protein isoform 2 precursor | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC110520.12. On Mar 28, 2018 this sequence version replaced NM_007424.2. Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849379 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGACCACTT TACTCTTGGT CTTTGTGACT CTGAGGGTCA TCGCTGCAGT GATCTCAGAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_031450.2 |
| CDS | 391..6789 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_007424.3 |
1 | ATGACCACTT TACTCTTGGT CTTTGTGACT CTGAGGGTCA TCGCTGCAGT GATCTCAGAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu38638 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006540566.1 , NM_001361500.1 | |
| Accession Version | NM_001361500.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 6513bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-30 | |
| Organism | Mus musculus(house mouse) | |
| Product | aggrecan core protein isoform 1 precursor | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC110520.12. On Mar 28, 2018 this sequence version replaced XM_006540566.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN00849374, SAMN01164131 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGACCACTT TACTCTTGGT CTTTGTGACT CTGAGGGTCA TCGCTGCAGT GATCTCAGAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001348429.1 |
| CDS | 391..6903 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001361500.1 |
1 | ATGACCACTT TACTCTTGGT CTTTGTGACT CTGAGGGTCA TCGCTGCAGT GATCTCAGAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OMu38638 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006540566.1 , NM_001361500.1 | |
| Accession Version | XM_006540566.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 6513bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-21 | |
| Organism | Mus musculus(house mouse) | |
| Product | aggrecan core protein isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGACCACTT TACTCTTGGT CTTTGTGACT CTGAGGGTCA TCGCTGCAGT GATCTCAGAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006540629.1 |
| CDS | 415..6927 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006540566.1 |
1 | ATGACCACTT TACTCTTGGT CTTTGTGACT CTGAGGGTCA TCGCTGCAGT GATCTCAGAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
