Cldn16 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cldn16
Entrez Gene ID	114141
Full Name	claudin 16
Synonyms	PCLN1,claudin-16
General protein information	Preferred Names claudin 16 Names claudin-16 H59D2a protein paracellin-1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 16 Map Location: 16\|16 B2
Summary	This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The protein encoded by this gene is critical for renal paracellular epithelial transport of Ca(2+) and Mg(2+) in the thick ascending loop of Henle. The gene deficiency leads to specific alterations in renal Ca(2+) and Mg(2+) balance and also to disturbances in Na(+) handling. The interaction of this gene and the Cldn 19 gene is required for their assembly into tight junctions and for renal Mg(2+) reabsorption. This gene and the Cldn1 gene are clustered on chromosome 16. [provided by RefSeq, Aug 2010].

mRNA and Protein(s)

mRNA	Protein	Name
NM_053241.5	NP_444471.1	claudin-16

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CLDN16	XP_001161102.1
Bos taurus (cattle)	CLDN16	NP_776944.1
Rattus norvegicus (Norway rat)	Cldn16	NP_571980.1
Gallus gallus (chicken)	CLDN16	XP_426702.1
Canis lupus familiaris (dog)	CLDN16	XP_850268.2
Mus musculus (house mouse)	Cldn16	NP_444471.1
Homo sapiens (human)	CLDN16	NP_006571.1
Xenopus tropicalis (tropical clawed frog)	cldn16	XP_002934087.1

Gene Ontology
Bibliography

Related articles in PubMed

Deletion of claudin-10 rescues claudin-16-deficient mice from hypomagnesemia and hypercalciuria.
Breiderhoff T, Himmerkus N, Drewell H, Plain A, G?nzel D, Mutig K, Willnow TE, M?ller D, Bleich M
Kidney international(2017 Nov)

Mosaic expression of claudins in thick ascending limbs of Henle results in spatial separation of paracellular Na+ and Mg2+ transport.
Milatz S, Himmerkus N, Wulfmeyer VC, Drewell H, Mutig K, Hou J, Breiderhoff T, M?ller D, Fromm M, Bleich M, G?nzel D
Proceedings of the National Academy of Sciences of the United States of America114(2)E219-E227(2017 Jan)

Claudin-16 Deficiency Impairs Tight Junction Function in Ameloblasts, Leading to Abnormal Enamel Formation.
Bardet C, Courson F, Wu Y, Khaddam M, Salmon B, Ribes S, Thumfart J, Yamaguti PM, Rochefort GY, Figueres ML, Breiderhoff T, Garcia-Casta?o A, Vall?e B, Le Denmat D, Baroukh B, Guilbert T, Schmitt A, Mass? JM, Bazin D, Lorenz G, Morawietz M, Hou J, Carvalho-Lobato P, Manzanares MC, Fricain JC, Talmud D, Demontis R, Neves F, Zenaty D, Berdal A, Kiesow A, Petzold M, Menashi S, Linglart A, Acevedo AC, Vargas-Poussou R, M?ller D, Houillier P, Chaussain C
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research31(3)498-513(2016 Mar)

A gene expression resource generated by genome-wide lacZ profiling in the mouse.
Tuck E, Estabel J, Oellrich A, Maguire AK, Adissu HA, Souter L, Siragher E, Lillistone C, Green AL, Wardle-Jones H, Carragher DM, Karp NA, Smedley D, Adams NC, , Bussell JN, Adams DJ, Ram?rez-Solis R, Steel KP, Galli A, White JK
Disease models & mechanisms8(11)1467-78(2015 Nov)

The claudin-16 channel gene is transcriptionally inhibited by 1,25-dihydroxyvitamin?D.
Kladnitsky O, Rozenfeld J, Azulay-Debby H, Efrati E, Zelikovic I
Experimental physiology100(1)79-94(2015 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

claudin-16 gene (CLDN16) mutations result in amelogenesis imperfect.
Title: Claudin-16 Deficiency Impairs Tight Junction Function in Ameloblasts, Leading to Abnormal Enamel Formation.

1,25(OH)2 VitD transcriptionally inhibits renal claudin-16 expression by a mechanism sensitive to CaSR and Mg(2+).
Title: The claudin-16 channel gene is transcriptionally inhibited by 1,25-dihydroxyvitamin?D.

Mg(2+)-loaded animals displayed hypermagnesemia with increasing urine Mg(2+)/Ca(2+) levels paralleled by a decrease in claudin-16 protein and mRNA in the kidney.
Title: Transcriptional regulation of the claudin-16 gene by Mg2+ availability.

Claudin-16 and claudin-19 interaction is required for their assembly into tight junctions and for renal reabsorption of magnesium.
Title: Claudin-16 and claudin-19 interaction is required for their assembly into tight junctions and for renal reabsorption of magnesium.

Insights into driving forces and paracellular permeability from claudin-16 knockdown mouse
Title: Insights into driving forces and paracellular permeability from claudin-16 knockdown mouse.

The following Cldn16 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cldn16 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu09417	NM_053241.5 Latest version!	Mus musculus claudin 16 (Cldn16), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cldn16 ( NM_053241.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_444471.1 Mus musculus claudin 16 (Cldn16), mRNA.

Price & Availability↑

CloneID	OMu09417
Clone ID Related Accession (Same CDS sequence)	NM_053241.5
Accession Version	NM_053241.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	claudin-16
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK085268.1 and AK085333.1. On Aug 11, 2010 this sequence version replaced NM_053241.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK085333.1, AK085268.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849385 [ECO:0000348] ##Evidence-Data-END##

ATGAAGGATC TTCTTCAGTA CGCTGCCTGC TTCTTGGCCA TATTCTCCAC TGGGTTTTTG 
ATCGTGGCCA CCTGGACAGA CTGTTGGATG GTGAACGCTG ATGACTCCCT GGAGGTGAGC 
ACTAAATGCA GAGGCCTGTG GTGGGAGTGT GTAACAAACG CTTTTGATGG GATTCGAACC 
TGCGATGAGT ACGACTCCAT ATATGCAGAA CATCCCTTGA AGCTGGTGGT AACTCGAGCA 
CTGATGATCA CAGCTGACAT TTTAGCTGGC TTTGGATTCA TCACCCTGCT CCTTGGTCTG 
GACTGTGTGA AGTTCCTACC TGATGACCCA CAAATTAAAG TCCGCCTTTG CTTTGTTGCA 
GGGACCACAT TACTCATTGC AGGTACCCCA GGAATCATCG GTTCTGTGTG GTATGCTGTG 
GATGTTTACG TCGAACGCTC CTCTCTCGTT TTACACAATA TATTTCTTGG GATCCAATAT 
AAATTTGGTT GGTCCTGCTG GCTTGGAATG GCTGGGTCTT TGGGTTGCTT TTTGGCAGGA 
GCTCTCCTCA CCTGCTGTTT GTACCTCTTC AAAGATGTTG GGCCTGAGAG GAACTACCCT 
TATGCCATGA GGAAGCCCTA TTCAACTGCA GGTGTGTCCA TGGCCAAGTC CTACAAGGCC 
CCTCGGACAG AGACAGCCAA AATGTATGCT GTAGACACCA GAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_444471.1
CDS	352..1059
Translation	MKDLLQYAACFLAIFSTGFLIVATWTDCWMVNADDSLEVSTKCRGLWWECVTNAFDGIRTCDEYDSIYAEHPLKLVVTRALMITADILAGFGFITLLLGLDCVKFLPDDPQIKVRLCFVAGTTLLIAGTPGIIGSVWYAVDVYVERSSLVLHNIFLGIQYKFGWSCWLGMAGSLGCFLAGALLTCCLYLFKDVGPERNYPYAMRKPYSTAGVSMAKSYKAPRTETAKMYAVDTRV

Target ORF information:

RefSeq Version	NM_053241.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus claudin 16 (Cldn16), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_053241.5

ORF Insert Sequence:

ATGAAGGATC TTCTTCAGTA CGCTGCCTGC TTCTTGGCCA TATTCTCCAC TGGGTTTTTG 
ATCGTGGCCA CCTGGACAGA CTGTTGGATG GTGAACGCTG ATGACTCCCT GGAGGTGAGC 
ACTAAATGCA GAGGCCTGTG GTGGGAGTGT GTAACAAACG CTTTTGATGG GATTCGAACC 
TGCGATGAGT ACGACTCCAT ATATGCAGAA CATCCCTTGA AGCTGGTGGT AACTCGAGCA 
CTGATGATCA CAGCTGACAT TTTAGCTGGC TTTGGATTCA TCACCCTGCT CCTTGGTCTG 
GACTGTGTGA AGTTCCTACC TGATGACCCA CAAATTAAAG TCCGCCTTTG CTTTGTTGCA 
GGGACCACAT TACTCATTGC AGGTACCCCA GGAATCATCG GTTCTGTGTG GTATGCTGTG 
GATGTTTACG TCGAACGCTC CTCTCTCGTT TTACACAATA TATTTCTTGG GATCCAATAT 
AAATTTGGTT GGTCCTGCTG GCTTGGAATG GCTGGGTCTT TGGGTTGCTT TTTGGCAGGA 
GCTCTCCTCA CCTGCTGTTT GTACCTCTTC AAAGATGTTG GGCCTGAGAG GAACTACCCT 
TATGCCATGA GGAAGCCCTA TTCAACTGCA GGTGTGTCCA TGGCCAAGTC CTACAAGGCC 
CCTCGGACAG AGACAGCCAA AATGTATGCT GTAGACACCA GAGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Deletion of claudin-10 rescues claudin-16-deficient mice from hypomagnesemia and hypercalciuria.
Kidney international(2017 Nov)
Breiderhoff T,Himmerkus N,Drewell H,Plain A,G?nzel D,Mutig K,Willnow TE,M?ller D,Bleich M

Mosaic expression of claudins in thick ascending limbs of Henle results in spatial separation of paracellular Na+ and Mg2+ transport.
Proceedings of the National Academy of Sciences of the United States of America114(2)E219-E227(2017 Jan)
Milatz S,Himmerkus N,Wulfmeyer VC,Drewell H,Mutig K,Hou J,Breiderhoff T,M?ller D,Fromm M,Bleich M,G?nzel D

Claudin-16 Deficiency Impairs Tight Junction Function in Ameloblasts, Leading to Abnormal Enamel Formation.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research31(3)498-513(2016 Mar)
Bardet C,Courson F,Wu Y,Khaddam M,Salmon B,Ribes S,Thumfart J,Yamaguti PM,Rochefort GY,Figueres ML,Breiderhoff T,Garcia-Casta?o A,Vall?e B,Le Denmat D,Baroukh B,Guilbert T,Schmitt A,Mass? JM,Bazin D,Lorenz G,Morawietz M,Hou J,Carvalho-Lobato P,Manzanares MC,Fricain JC,Talmud D,Demontis R,Neves F,Zenaty D,Berdal A,Kiesow A,Petzold M,Menashi S,Linglart A,Acevedo AC,Vargas-Poussou R,M?ller D,Houillier P,Chaussain C

A gene expression resource generated by genome-wide lacZ profiling in the mouse.
Disease models & mechanisms8(11)1467-78(2015 Nov)
Tuck E,Estabel J,Oellrich A,Maguire AK,Adissu HA,Souter L,Siragher E,Lillistone C,Green AL,Wardle-Jones H,Carragher DM,Karp NA,Smedley D,Adams NC,,Bussell JN,Adams DJ,Ram?rez-Solis R,Steel KP,Galli A,White JK

The claudin-16 channel gene is transcriptionally inhibited by 1,25-dihydroxyvitamin?D.
Experimental physiology100(1)79-94(2015 Jan)
Kladnitsky O,Rozenfeld J,Azulay-Debby H,Efrati E,Zelikovic I

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Cldn16 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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