CLDN16 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CLDN16
Entrez Gene ID	10686
Full Name	claudin 16
Synonyms	HOMG3,PCLN1
General protein information	Preferred Names claudin 16 Names claudin-16 PCLN-1 hypomagnesemia 3, with hypercalciuria and nephrocalcinosis paracellin-1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q28
Summary	Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is found primarily in the kidneys, specifically in the thick ascending limb of Henle, where it acts as either an intercellular pore or ion concentration sensor to regulate the paracellular resorption of magnesium ions. Defects in this gene are a cause of primary hypomagnesemia, which is characterized by massive renal magnesium wasting with hypomagnesemia and hypercalciuria, resulting in nephrocalcinosis and renal failure. This gene and the CLDN1 gene are clustered on chromosome 3q28. [provided by RefSeq, Jun 2010].
Disorder MIM:	603959
Disorder Html:	Hypomagnesemia 3, renal, 248250 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_006580.3	NP_006571.1	claudin-16

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CLDN16	XP_001161102.1
Bos taurus (cattle)	CLDN16	NP_776944.1
Rattus norvegicus (Norway rat)	Cldn16	NP_571980.1
Gallus gallus (chicken)	CLDN16	XP_426702.1
Canis lupus familiaris (dog)	CLDN16	XP_850268.2
Mus musculus (house mouse)	Cldn16	NP_444471.1
Homo sapiens (human)	CLDN16	NP_006571.1
Xenopus tropicalis (tropical clawed frog)	cldn16	XP_002934087.1

Gene Ontology
Bibliography

Related articles in PubMed

The RING finger- and PDZ domain-containing protein PDZRN3 controls localization of the Mg
Marunaka K, Furukawa C, Fujii N, Kimura T, Furuta T, Matsunaga T, Endo S, Hasegawa H, Anzai N, Yamazaki Y, Yamaguchi M, Ikari A
The Journal of biological chemistry292(31)13034-13044(2017 08)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that Pdzrn3 mediates endocytosis of dephosphorylated CLDN16 and represents an important component of CLDN16-trafficking machinery in renal tube epithelial cells. (Pdzrn3 = PDZ domain containing RING finger 3 protein; CLDN16 = claudin 16)
Title: The RING finger- and PDZ domain-containing protein PDZRN3 controls localization of the Mg

detected a novel mutation in CLDN16 for the first time. The clinical and genetic findings from this study will help to expand the understanding of this rare disease, FHHNC
Title: A novel mutation in CLDN16 results in rare familial hypomagnesaemia with hypercalciuria and nephrocalcinosis in a Chinese family.

claudin-16 gene (CLDN16) mutations result in amelogenesis imperfect.
Title: Claudin-16 Deficiency Impairs Tight Junction Function in Ameloblasts, Leading to Abnormal Enamel Formation.

CLDN16 mutations are associated with familial hypomagnesaemia with hypercalciuria and nephrocalcinosis.
Title: Retrospective cohort study of familial hypomagnesaemia with hypercalciuria and nephrocalcinosis due to CLDN16 mutations.

1,25(OH)2 VitD transcriptionally inhibits renal claudin-16 expression by a mechanism sensitive to CaSR and Mg(2+).
Title: The claudin-16 channel gene is transcriptionally inhibited by 1,25-dihydroxyvitamin?D.

The following CLDN16 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CLDN16 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu21662	NM_006580.3 Latest version!	Homo sapiens claudin 16 (CLDN16), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CLDN16 ( NM_006580.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_006571.1 Homo sapiens claudin 16 (CLDN16), mRNA.

Price & Availability↑

CloneID	OHu21662
Clone ID Related Accession (Same CDS sequence)	NM_006580.3
Accession Version	NM_006580.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	claudin-16
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK314955.1, BC069662.1, AC009520.16 and CB241927.1. This sequence is a reference standard in the RefSeqGene project. On Jun 4, 2010 this sequence version replaced NM_006580.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK314955.1, BC069662.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGACCTCCA GGACCCCACT GTTGGTTACA GCCTGTTTGT ATTATTCTTA CTGCAACTCA 
AGACACCTGC AGCAGGGCGT GAGAAAAAGT AAAAGACCAG TATTTTCACA TTGCCAGGTA 
CCAGAAACAC AGAAGACTGA CACCCGCCAC TTAAGTGGGG CCAGGGCTGG TGTCTGCCCA 
TGTTGCCATC CTGATGGGCT GCTTGCCACA ATGAGGGATC TTCTTCAATA CATCGCTTGC 
TTCTTTGCCT TTTTCTCTGC TGGGTTTTTG ATTGTGGCCA CCTGGACTGA CTGTTGGATG 
GTGAATGCTG ATGACTCTCT GGAGGTGAGC ACAAAATGCC GAGGCCTCTG GTGGGAATGC 
GTCACAAATG CTTTTGATGG GATTCGCACC TGTGATGAGT ACGATTCCAT ACTTGCGGAG 
CATCCCTTGA AGCTGGTGGT AACTCGAGCG TTGATGATTA CTGCAGATAT TCTAGCTGGG 
TTTGGATTTC TCACCCTGCT CCTTGGTCTT GACTGCGTGA AATTCCTCCC TGATGAGCCG 
TACATTAAAG TCCGCATCTG CTTTGTTGCT GGAGCCACGT TACTAATAGC AGGTACCCCA 
GGAATCATTG GCTCTGTGTG GTATGCTGTT GATGTGTATG TGGAACGTTC TACTTTGGTT 
TTGCACAATA TATTTCTTGG TATCCAATAT AAATTTGGTT GGTCCTGTTG GCTCGGAATG 
GCTGGGTCTC TGGGTTGCTT TTTGGCTGGA GCTGTTCTCA CCTGCTGCTT ATATCTTTTT 
AAAGATGTTG GACCTGAGAG AAACTATCCT TATTCCTTGA GGAAAGCCTA TTCAGCCGCG 
GGTGTTTCCA TGGCCAAGTC ATACTCAGCC CCTCGCACAG AGACGGCCAA AATGTATGCT 
GTAGACACAA GGGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_006571.1
CDS	249..1166
Translation	MTSRTPLLVTACLYYSYCNSRHLQQGVRKSKRPVFSHCQVPETQKTDTRHLSGARAGVCPCCHPDGLLATMRDLLQYIACFFAFFSAGFLIVATWTDCWMVNADDSLEVSTKCRGLWWECVTNAFDGIRTCDEYDSILAEHPLKLVVTRALMITADILAGFGFLTLLLGLDCVKFLPDEPYIKVRICFVAGATLLIAGTPGIIGSVWYAVDVYVERSTLVLHNIFLGIQYKFGWSCWLGMAGSLGCFLAGAVLTCCLYLFKDVGPERNYPYSLRKAYSAAGVSMAKSYSAPRTETAKMYAVDTRV

Target ORF information:

RefSeq Version	NM_006580.3
Organism	Homo sapiens(human)
Definition	Homo sapiens claudin 16 (CLDN16), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006580.3

ORF Insert Sequence:

ATGACCTCCA GGACCCCACT GTTGGTTACA GCCTGTTTGT ATTATTCTTA CTGCAACTCA 
AGACACCTGC AGCAGGGCGT GAGAAAAAGT AAAAGACCAG TATTTTCACA TTGCCAGGTA 
CCAGAAACAC AGAAGACTGA CACCCGCCAC TTAAGTGGGG CCAGGGCTGG TGTCTGCCCA 
TGTTGCCATC CTGATGGGCT GCTTGCCACA ATGAGGGATC TTCTTCAATA CATCGCTTGC 
TTCTTTGCCT TTTTCTCTGC TGGGTTTTTG ATTGTGGCCA CCTGGACTGA CTGTTGGATG 
GTGAATGCTG ATGACTCTCT GGAGGTGAGC ACAAAATGCC GAGGCCTCTG GTGGGAATGC 
GTCACAAATG CTTTTGATGG GATTCGCACC TGTGATGAGT ACGATTCCAT ACTTGCGGAG 
CATCCCTTGA AGCTGGTGGT AACTCGAGCG TTGATGATTA CTGCAGATAT TCTAGCTGGG 
TTTGGATTTC TCACCCTGCT CCTTGGTCTT GACTGCGTGA AATTCCTCCC TGATGAGCCG 
TACATTAAAG TCCGCATCTG CTTTGTTGCT GGAGCCACGT TACTAATAGC AGGTACCCCA 
GGAATCATTG GCTCTGTGTG GTATGCTGTT GATGTGTATG TGGAACGTTC TACTTTGGTT 
TTGCACAATA TATTTCTTGG TATCCAATAT AAATTTGGTT GGTCCTGTTG GCTCGGAATG 
GCTGGGTCTC TGGGTTGCTT TTTGGCTGGA GCTGTTCTCA CCTGCTGCTT ATATCTTTTT 
AAAGATGTTG GACCTGAGAG AAACTATCCT TATTCCTTGA GGAAAGCCTA TTCAGCCGCG 
GGTGTTTCCA TGGCCAAGTC ATACTCAGCC CCTCGCACAG AGACGGCCAA AATGTATGCT 
GTAGACACAA GGGTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The RING finger- and PDZ domain-containing protein PDZRN3 controls localization of the Mg
The Journal of biological chemistry292(31)13034-13044(2017 08)
Marunaka K,Furukawa C,Fujii N,Kimura T,Furuta T,Matsunaga T,Endo S,Hasegawa H,Anzai N,Yamazaki Y,Yamaguchi M,Ikari A

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CLDN16 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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