Fbxo4 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fbxo4
Entrez Gene ID	106052
Full Name	F-box protein 4
Synonyms	1700096C12Rik,AI851261,AW494535,Fbx4
General protein information	Preferred Names F-box protein 4 Names F-box only protein 4
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 15 Map Location: 15\|15 A1

mRNA and Protein(s)

mRNA	Protein	Name
NM_134099.2	NP_598860.2	F-box only protein 4

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	fbxo4	NP_001015940.1
Pan troglodytes (chimpanzee)	FBXO4	XP_001137034.1
Bos taurus (cattle)	FBXO4	NP_001029789.1
Mus musculus (house mouse)	Fbxo4	NP_598860.2
Danio rerio (zebrafish)	si:dkey-46a10.2	XP_002663185.3
Homo sapiens (human)	FBXO4	NP_036308.1
	FBXO4	XP_001088308.1
Canis lupus familiaris (dog)	FBXO4	XP_003639033.1
Rattus norvegicus (Norway rat)	Fbxo4	NP_001101142.1
Gallus gallus (chicken)	FBXO4	XP_429141.3

Gene Ontology
Bibliography

Related articles in PubMed

Fbxo4-mediated degradation of Fxr1 suppresses tumorigenesis in head and neck squamous cell carcinoma.
Qie S, Majumder M, Mackiewicz K, Howley BV, Peterson YK, Howe PH, Palanisamy V, Diehl JA
Nature communications8(1)1534(2017 Nov)

PERK Is a Haploinsufficient Tumor Suppressor: Gene Dose Determines Tumor-Suppressive Versus Tumor Promoting Properties of PERK in Melanoma.
Pytel D, Gao Y, Mackiewicz K, Katlinskaya YV, Staschke KA, Paredes MC, Yoshida A, Qie S, Zhang G, Chajewski OS, Wu L, Majsterek I, Herlyn M, Fuchs SY, Diehl JA
PLoS genetics12(12)e1006518(2016 Dec)

FBXO4 loss facilitates carcinogen induced papilloma development in mice.
Lian Z, Lee EK, Bass AJ, Wong KK, Klein-Szanto AJ, Rustgi AK, Diehl JA
Cancer biology & therapy16(5)750-5(2015)

The FBXO4 tumor suppressor functions as a barrier to BRAFV600E-dependent metastatic melanoma.
Lee EK, Lian Z, D'Andrea K, Letrero R, Sheng W, Liu S, Diehl JN, Pytel D, Barbash O, Schuchter L, Amaravaradi R, Xu X, Herlyn M, Nathanson KL, Diehl JA
Molecular and cellular biology33(22)4422-33(2013 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Collectively, our data support a role for FBXO4 as a suppressor of esophageal tumorigenesis
Title: FBXO4 loss facilitates carcinogen induced papilloma development in mice.

This study demonstrates that Fbxo4 deficiency induces Braf-driven melanoma and that this phenotype depends on cyclin D1 accumulation in mice, underscoring the importance of this ubiquitin ligase in tumor suppression.
Title: The FBXO4 tumor suppressor functions as a barrier to BRAFV600E-dependent metastatic melanoma.

Fbx4 expression is essential for cell homeostasis in somatic cells. Fbx4 ablation predisposes mice to multiple tumor phenotypes, with concomitant cyclin D1 deregulation and nuclear accumulation
Title: The Fbx4 tumor suppressor regulates cyclin D1 accumulation and prevents neoplastic transformation.

Lysine 269 is essential for cyclin D1 ubiquitylation by the SCF(Fbx4/alphaB-crystallin) ligase.
Title: Lysine 269 is essential for cyclin D1 ubiquitylation by the SCF(Fbx4/alphaB-crystallin) ligase and subsequent proteasome-dependent degradation.

SCF(FBX4-alphaB crystallin) is an E3 ubiquitin ligase that promotes ubiquitin-dependent degradation of Thr286-phosphorylated cyclin D1.
Title: Phosphorylation-dependent ubiquitination of cyclin D1 by the SCF(FBX4-alphaB crystallin) complex.

The following Fbxo4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fbxo4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu10538	NM_134099.2 Latest version!	Mus musculus F-box protein 4 (Fbxo4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$188.30 $269.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fbxo4 ( NM_134099.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598860.2 Mus musculus F-box protein 4 (Fbxo4), mRNA.

Price & Availability↑

CloneID	OMu10538
Clone ID Related Accession (Same CDS sequence)	NM_134099.2
Accession Version	NM_134099.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Mus musculus(house mouse)
Product	F-box only protein 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC137902.10, BC036385.1 and AI480454.1. On Aug 27, 2009 this sequence version replaced NM_134099.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC040086.1, SRR7345562.1029933.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849382, SAMN01164134 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGAA GCGAGCCCCG CGGAGCCGGC TCCCCGCCGC CCGCCAGCGA CTGGGGCCGC 
CTGGAGGCAG CCATCCTGAG CGGCTGGAGG ACCTTCTGGT ATTCGGTGGC CAAGGAGCGG 
GCGACGCCGA CGGCCTCCCG GAAGGAGGCG GCGGAGGAGA CGAGCGCGCT GACGCGGCTG 
CCGGTTGATG TGCAGTTGTA TATCTTGTCG TTTCTTTCAC CCCACGATCT GTGCCAGCTG 
GGAAGTACAG ATCATTACTG GAACAAAACT ATAAGAGACC CAATTCTCTG GAGATACTTT 
CTGTTGCGGG ATCTCCCTTC TTGGTCTTCG GTTGATTGGA AGTCACTTCC AGATCTAGAG 
ATCTTAAAAA AGCCAATATC TGAGGTCACC GACAGCACTT GTCTTGATTA CATGGAGGTT 
TATAAAATGT GCTGTCCATA TACGCGAAGA GCCTTGAAAG CCAGCCGTCC TATGTATGGA 
GTGGTTACCT CTTTCTTACA CTCACTGATC ATTCAGAATG AACCCCGGTT TGCTATGTTT 
GGACCAGGTT TGGAAGAACT GAACACATCC TTGGTGTTGA GTTTGATGTC TTCTGAGGAC 
CTTTGCCCAA CTGCTGGTTT ACCTCACAGA CAGATTGATG GTATTGGATC TGGAGTCAAC 
TTCCAGTTGA ATAACCAGCA AAAATTCAAC ATCCTGATAT TATACTCGAC TACCAGAAAA 
GAAAGAGACA GAGCAAGGGA GGAGCACACC AGCACCGTTA ACAAGATGTT CAGCCTACAG 
AGTGAGGGGG ACGAGCAGCA GGGCAGCCGC TACAGTGTGA TCCCGCAGAT TCAGAAAGTG 
TGTGAAGTCG TAGACGGGTT CATCTACGTG GCAAACGCTG AAGCTCACCG ACGTCATGAA 
TGGCAAGATG AATTTTCTCG GATTATGGCC ATGACAGACC CAGCTTTTGG ATCTTCAGGA 
AGACCCATGC TGGTTTTATC TTGTATTTCT CAAGCAGATG TAAAGAGAAT GCCTTGTTTT 
TATTTAGCTC ATGAGCTGCA CCTCAGTCTT CTAAACCACC CATGGATGGT CCAGGATACA 
GAGGCTGAAA CTCTGACTGG TTTTTTGAAT GGCATTGAGT GGATTCTTGA AGAAGTAGAA 
TCTAAGCGTG CAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598860.2
CDS	150..1307
Translation	MAGSEPRGAGSPPPASDWGRLEAAILSGWRTFWYSVAKERATPTASRKEAAEETSALTRLPVDVQLYILSFLSPHDLCQLGSTDHYWNKTIRDPILWRYFLLRDLPSWSSVDWKSLPDLEILKKPISEVTDSTCLDYMEVYKMCCPYTRRALKASRPMYGVVTSFLHSLIIQNEPRFAMFGPGLEELNTSLVLSLMSSEDLCPTAGLPHRQIDGIGSGVNFQLNNQQKFNILILYSTTRKERDRAREEHTSTVNKMFSLQSEGDEQQGSRYSVIPQIQKVCEVVDGFIYVANAEAHRRHEWQDEFSRIMAMTDPAFGSSGRPMLVLSCISQADVKRMPCFYLAHELHLSLLNHPWMVQDTEAETLTGFLNGIEWILEEVESKRAK

Target ORF information:

RefSeq Version	NM_134099.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus F-box protein 4 (Fbxo4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_134099.2

ORF Insert Sequence:

ATGGCTGGAA GCGAGCCCCG CGGAGCCGGC TCCCCGCCGC CCGCCAGCGA CTGGGGCCGC 
CTGGAGGCAG CCATCCTGAG CGGCTGGAGG ACCTTCTGGT ATTCGGTGGC CAAGGAGCGG 
GCGACGCCGA CGGCCTCCCG GAAGGAGGCG GCGGAGGAGA CGAGCGCGCT GACGCGGCTG 
CCGGTTGATG TGCAGTTGTA TATCTTGTCG TTTCTTTCAC CCCACGATCT GTGCCAGCTG 
GGAAGTACAG ATCATTACTG GAACAAAACT ATAAGAGACC CAATTCTCTG GAGATACTTT 
CTGTTGCGGG ATCTCCCTTC TTGGTCTTCG GTTGATTGGA AGTCACTTCC AGATCTAGAG 
ATCTTAAAAA AGCCAATATC TGAGGTCACC GACAGCACTT GTCTTGATTA CATGGAGGTT 
TATAAAATGT GCTGTCCATA TACGCGAAGA GCCTTGAAAG CCAGCCGTCC TATGTATGGA 
GTGGTTACCT CTTTCTTACA CTCACTGATC ATTCAGAATG AACCCCGGTT TGCTATGTTT 
GGACCAGGTT TGGAAGAACT GAACACATCC TTGGTGTTGA GTTTGATGTC TTCTGAGGAC 
CTTTGCCCAA CTGCTGGTTT ACCTCACAGA CAGATTGATG GTATTGGATC TGGAGTCAAC 
TTCCAGTTGA ATAACCAGCA AAAATTCAAC ATCCTGATAT TATACTCGAC TACCAGAAAA 
GAAAGAGACA GAGCAAGGGA GGAGCACACC AGCACCGTTA ACAAGATGTT CAGCCTACAG 
AGTGAGGGGG ACGAGCAGCA GGGCAGCCGC TACAGTGTGA TCCCGCAGAT TCAGAAAGTG 
TGTGAAGTCG TAGACGGGTT CATCTACGTG GCAAACGCTG AAGCTCACCG ACGTCATGAA 
TGGCAAGATG AATTTTCTCG GATTATGGCC ATGACAGACC CAGCTTTTGG ATCTTCAGGA 
AGACCCATGC TGGTTTTATC TTGTATTTCT CAAGCAGATG TAAAGAGAAT GCCTTGTTTT 
TATTTAGCTC ATGAGCTGCA CCTCAGTCTT CTAAACCACC CATGGATGGT CCAGGATACA 
GAGGCTGAAA CTCTGACTGG TTTTTTGAAT GGCATTGAGT GGATTCTTGA AGAAGTAGAA 
TCTAAGCGTG CAAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Fbxo4-mediated degradation of Fxr1 suppresses tumorigenesis in head and neck squamous cell carcinoma.
Nature communications8(1)1534(2017 Nov)
Qie S,Majumder M,Mackiewicz K,Howley BV,Peterson YK,Howe PH,Palanisamy V,Diehl JA

PERK Is a Haploinsufficient Tumor Suppressor: Gene Dose Determines Tumor-Suppressive Versus Tumor Promoting Properties of PERK in Melanoma.
PLoS genetics12(12)e1006518(2016 Dec)
Pytel D,Gao Y,Mackiewicz K,Katlinskaya YV,Staschke KA,Paredes MC,Yoshida A,Qie S,Zhang G,Chajewski OS,Wu L,Majsterek I,Herlyn M,Fuchs SY,Diehl JA

FBXO4 loss facilitates carcinogen induced papilloma development in mice.
Cancer biology & therapy16(5)750-5(2015)
Lian Z,Lee EK,Bass AJ,Wong KK,Klein-Szanto AJ,Rustgi AK,Diehl JA

The FBXO4 tumor suppressor functions as a barrier to BRAFV600E-dependent metastatic melanoma.
Molecular and cellular biology33(22)4422-33(2013 Nov)
Lee EK,Lian Z,D'Andrea K,Letrero R,Sheng W,Liu S,Diehl JN,Pytel D,Barbash O,Schuchter L,Amaravaradi R,Xu X,Herlyn M,Nathanson KL,Diehl JA

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Fbxo4 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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