Hsd3b7 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hsd3b7
Entrez Gene ID	101502
Full Name	hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7
Synonyms	AI195443,BB098564
General protein information	Preferred Names hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 Names 3 beta-hydroxysteroid dehydrogenase type 7 3 beta-hydroxysteroid dehydrogenase type VII 3-beta-HSD VII 3-beta-hydroxy-Delta(5)-C27 steroid oxidoreductase c(27) 3-beta-HSD cholest-5-ene-3-beta,7-alpha-diol 3-beta-dehydrogenase
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7\|7 F3

mRNA and Protein(s)

mRNA	Protein	Name
XM_017321905.1	XP_017177394.1	3 beta-hydroxysteroid dehydrogenase type 7 isoform X1
XM_017321906.1	XP_017177395.1	3 beta-hydroxysteroid dehydrogenase type 7 isoform X1
NM_001040684.1	NP_001035774.1	3 beta-hydroxysteroid dehydrogenase type 7 isoform b
NM_133943.2	NP_598704.2	3 beta-hydroxysteroid dehydrogenase type 7 isoform a

Pathways from BioSystems

Homologies

Homo sapiens (human)	HSD3B7	NP_079469.2
Rattus norvegicus (Norway rat)	Hsd3b7	NP_647545.1
Xenopus tropicalis (tropical clawed frog)	hsd3b7	NP_001120501.1
Bos taurus (cattle)	HSD3B7	NP_001029868.1
Danio rerio (zebrafish)	hsd3b7	NP_956103.1
Pan troglodytes (chimpanzee)	HSD3B7	XP_001155219.1
Canis lupus familiaris (dog)	HSD3B7	XP_547037.1
Mus musculus (house mouse)	Hsd3b7	NP_598704.2

Gene Ontology
Bibliography

Related articles in PubMed

EBI2 augments Tfh cell fate by promoting interaction with IL-2-quenching dendritic cells.
Li J, Lu E, Yi T, Cyster JG
Nature533(7601)110-4(2016 May)

EBI2-mediated bridging channel positioning supports splenic dendritic cell homeostasis and particulate antigen capture.
Yi T, Cyster JG
eLife2e00757(2013 May)

Oxysterol gradient generation by lymphoid stromal cells guides activated B cell movement during humoral responses.
Yi T, Wang X, Kelly LM, An J, Xu Y, Sailer AW, Gustafsson JA, Russell DW, Cyster JG
Immunity37(3)535-48(2012 Sep)

Analysis of HSD3B7 knockout mice reveals that a 3alpha-hydroxyl stereochemistry is required for bile acid function.
Shea HC, Head DD, Setchell KD, Russell DW
Proceedings of the National Academy of Sciences of the United States of America104(28)11526-33(2007 Jul)

The bile acid synthetic gene 3beta-hydroxy-Delta(5)-C(27)-steroid oxidoreductase is mutated in progressive intrahepatic cholestasis.
Schwarz M, Wright AC, Davis DL, Nazer H, Bj?rkhem I, Russell DW
The Journal of clinical investigation106(9)1175-84(2000 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Findings indicate that CYP7B1 and HSD3B7, as well as CH25H, have essential roles in controlling oxysterol production in lymphoid tissues.
Title: Oxysterol gradient generation by lymphoid stromal cells guides activated B cell movement during humoral responses.

Catalyzes two reactions required for inversion of the cholesterol 3beta-hydroxyl group to the 3alpha-hydroxyl of bile acids; its stereochemical alteration eliminates cholesterol absorption in the gut and enterohepatic circulation feedback regulation.
Title: Analysis of HSD3B7 knockout mice reveals that a 3alpha-hydroxyl stereochemistry is required for bile acid function.

The following Hsd3b7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hsd3b7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu18137	NM_001040684.1 Latest version!	Mus musculus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OMu18184	NM_133943.2 Latest version!	Mus musculus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OMu81883	XM_017321905.1 Latest version!	Mus musculus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OMu81883	XM_017321906.1 Latest version!	Mus musculus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hsd3b7 ( NM_001040684.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001035774.1 Mus musculus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu18137
Clone ID Related Accession (Same CDS sequence)	NM_001040684.1
Accession Version	NM_001040684.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-19
Organism	Mus musculus(house mouse)
Product	3 beta-hydroxysteroid dehydrogenase type 7 isoform b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB854484.1, BY133253.1, CB951288.1 and AC149222.4. ##Evidence-Data-START## CDS exon combination :: BC066039.1, AF277718.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGCTGG AACGGGAGCC CAGGCTCCGG GAGCTGCGTG TCTTTGACCT GCACCTGAGT 
TCTTGGCTGG AGGAGCTGAA AGCAGGGCCT GTGCAGGTGA CTGCCATCCA GGGGGATGTG 
ACTCAGGCCC ATGAGGTGGC AGCAGCCATG TCTGGATCAC ATGTGGTCAT CCATACAGCT 
GGGTTGGTGG ATGTGTTTGG GAAGGCCAGT CCAAAGACCA TCCACAAAGT CAACGTGCAG 
GGCACACAGA ATGTGATTGA TGCTTGTGTG CAGACTGGCA CTCAGTACCT GGTCTACACG 
AGCAGCATGG AAGTGGTGGG GCCTAACATC AAGGGCCACC CCTTCTACAG GGGCAATGAA 
GATACCCCAT ATGAGGCAGT CCACAGCCAT CCCTACCCAT GCAGTAAAGC CCTTGCTGAG 
CAGCTGGTCC TCGAGGCCAA TGGAAGGAAG GTCAATGGAG GGCTACCCCT GGTGACATGT 
GCCCTTCGAC CCACGGGCAT TTATGGTGAA GGTCATCAGG TCATGAGAGA CTTCTACTAC 
CAGGGACTGC GCTTTGGAGG TCGTCTATTT CGGGCCGTCC CAGCTTCTGT GGAGCACGGT 
CGGGTCTATG TTGGCAATGT TGCTTGGATG CACATACTGG TGGCCCGGGA GCTGGAGCAG 
CGGGCAGCAC TCATGGGTGG CCAGGTGTAT TTCTGCTATG ATAAGTCACC TTATAAAAGC 
TACGAGGACT TCAACATGGA GTTTCTGAGT CCCTGTGGTC TTCGACTGAT AGGCGCCCAC 
CCACTGCTGC CCTACTGGCT GCTAGTGCTG CTGGCTACCC TCAATGCCCT GCTGCAGTGG 
CTGCTCCGCC CACTGGTGCT GTACACACCC CTGCTGAATC CCTACACGCT GGCTATGGCC 
AACACCACCT TTACTGTCAG TACCAACAAG GCACAGCGGC ATTTTGGCTA CAAGCCCCTC 
TTCTCATGGG AAGAGAGCAG GACCCGCACC ATTCAGTGGG TGCAGGCGAT GGAGGGTTCA 
GCTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035774.1
CDS	142..1170
Translation	MLLEREPRLRELRVFDLHLSSWLEELKAGPVQVTAIQGDVTQAHEVAAAMSGSHVVIHTAGLVDVFGKASPKTIHKVNVQGTQNVIDACVQTGTQYLVYTSSMEVVGPNIKGHPFYRGNEDTPYEAVHSHPYPCSKALAEQLVLEANGRKVNGGLPLVTCALRPTGIYGEGHQVMRDFYYQGLRFGGRLFRAVPASVEHGRVYVGNVAWMHILVARELEQRAALMGGQVYFCYDKSPYKSYEDFNMEFLSPCGLRLIGAHPLLPYWLLVLLATLNALLQWLLRPLVLYTPLLNPYTLAMANTTFTVSTNKAQRHFGYKPLFSWEESRTRTIQWVQAMEGSAR

Target ORF information:

RefSeq Version	NM_001040684.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001040684.1

ORF Insert Sequence:

ATGCTGCTGG AACGGGAGCC CAGGCTCCGG GAGCTGCGTG TCTTTGACCT GCACCTGAGT 
TCTTGGCTGG AGGAGCTGAA AGCAGGGCCT GTGCAGGTGA CTGCCATCCA GGGGGATGTG 
ACTCAGGCCC ATGAGGTGGC AGCAGCCATG TCTGGATCAC ATGTGGTCAT CCATACAGCT 
GGGTTGGTGG ATGTGTTTGG GAAGGCCAGT CCAAAGACCA TCCACAAAGT CAACGTGCAG 
GGCACACAGA ATGTGATTGA TGCTTGTGTG CAGACTGGCA CTCAGTACCT GGTCTACACG 
AGCAGCATGG AAGTGGTGGG GCCTAACATC AAGGGCCACC CCTTCTACAG GGGCAATGAA 
GATACCCCAT ATGAGGCAGT CCACAGCCAT CCCTACCCAT GCAGTAAAGC CCTTGCTGAG 
CAGCTGGTCC TCGAGGCCAA TGGAAGGAAG GTCAATGGAG GGCTACCCCT GGTGACATGT 
GCCCTTCGAC CCACGGGCAT TTATGGTGAA GGTCATCAGG TCATGAGAGA CTTCTACTAC 
CAGGGACTGC GCTTTGGAGG TCGTCTATTT CGGGCCGTCC CAGCTTCTGT GGAGCACGGT 
CGGGTCTATG TTGGCAATGT TGCTTGGATG CACATACTGG TGGCCCGGGA GCTGGAGCAG 
CGGGCAGCAC TCATGGGTGG CCAGGTGTAT TTCTGCTATG ATAAGTCACC TTATAAAAGC 
TACGAGGACT TCAACATGGA GTTTCTGAGT CCCTGTGGTC TTCGACTGAT AGGCGCCCAC 
CCACTGCTGC CCTACTGGCT GCTAGTGCTG CTGGCTACCC TCAATGCCCT GCTGCAGTGG 
CTGCTCCGCC CACTGGTGCT GTACACACCC CTGCTGAATC CCTACACGCT GGCTATGGCC 
AACACCACCT TTACTGTCAG TACCAACAAG GCACAGCGGC ATTTTGGCTA CAAGCCCCTC 
TTCTCATGGG AAGAGAGCAG GACCCGCACC ATTCAGTGGG TGCAGGCGAT GGAGGGTTCA 
GCTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hsd3b7 ( NM_133943.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598704.2 Mus musculus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu18184
Clone ID Related Accession (Same CDS sequence)	NM_133943.2
Accession Version	NM_133943.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Mus musculus(house mouse)
Product	3 beta-hydroxysteroid dehydrogenase type 7 isoform a
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BB854484.1, CB951288.1 and AC149222.4. On May 24, 2006 this sequence version replaced NM_133943.1. ##Evidence-Data-START## Transcript exon combination :: AF277718.1, BC132605.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGACT CTGCACAGGT GCCAACACTG GTATACCTGG TCACAGGTGG CTGCGGCTTC 
CTGGGGGAAC ATATTGTTCG GATGCTGCTG GAACGGGAGC CCAGGCTCCG GGAGCTGCGT 
GTCTTTGACC TGCACCTGAG TTCTTGGCTG GAGGAGCTGA AAGCAGGGCC TGTGCAGGTG 
ACTGCCATCC AGGGGGATGT GACTCAGGCC CATGAGGTGG CAGCAGCCAT GTCTGGATCA 
CATGTGGTCA TCCATACAGC TGGGTTGGTG GATGTGTTTG GGAAGGCCAG TCCAAAGACC 
ATCCACAAAG TCAACGTGCA GGGCACACAG AATGTGATTG ATGCTTGTGT GCAGACTGGC 
ACTCAGTACC TGGTCTACAC GAGCAGCATG GAAGTGGTGG GGCCTAACAT CAAGGGCCAC 
CCCTTCTACA GGGGCAATGA AGATACCCCA TATGAGGCAG TCCACAGCCA TCCCTACCCA 
TGCAGTAAAG CCCTTGCTGA GCAGCTGGTC CTCGAGGCCA ATGGAAGGAA GGTCAATGGA 
GGGCTACCCC TGGTGACATG TGCCCTTCGA CCCACGGGCA TTTATGGTGA AGGTCATCAG 
GTCATGAGAG ACTTCTACTA CCAGGGACTG CGCTTTGGAG GTCGTCTATT TCGGGCCGTC 
CCAGCTTCTG TGGAGCACGG TCGGGTCTAT GTTGGCAATG TTGCTTGGAT GCACATACTG 
GTGGCCCGGG AGCTGGAGCA GCGGGCAGCA CTCATGGGTG GCCAGGTGTA TTTCTGCTAT 
GATAAGTCAC CTTATAAAAG CTACGAGGAC TTCAACATGG AGTTTCTGAG TCCCTGTGGT 
CTTCGACTGA TAGGCGCCCA CCCACTGCTG CCCTACTGGC TGCTAGTGCT GCTGGCTACC 
CTCAATGCCC TGCTGCAGTG GCTGCTCCGC CCACTGGTGC TGTACACACC CCTGCTGAAT 
CCCTACACGC TGGCTATGGC CAACACCACC TTTACTGTCA GTACCAACAA GGCACAGCGG 
CATTTTGGCT ACAAGCCCCT CTTCTCATGG GAAGAGAGCA GGACCCGCAC CATTCAGTGG 
GTGCAGGCGA TGGAGGGTTC AGCTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598704.2
CDS	113..1222
Translation	MADSAQVPTLVYLVTGGCGFLGEHIVRMLLEREPRLRELRVFDLHLSSWLEELKAGPVQVTAIQGDVTQAHEVAAAMSGSHVVIHTAGLVDVFGKASPKTIHKVNVQGTQNVIDACVQTGTQYLVYTSSMEVVGPNIKGHPFYRGNEDTPYEAVHSHPYPCSKALAEQLVLEANGRKVNGGLPLVTCALRPTGIYGEGHQVMRDFYYQGLRFGGRLFRAVPASVEHGRVYVGNVAWMHILVARELEQRAALMGGQVYFCYDKSPYKSYEDFNMEFLSPCGLRLIGAHPLLPYWLLVLLATLNALLQWLLRPLVLYTPLLNPYTLAMANTTFTVSTNKAQRHFGYKPLFSWEESRTRTIQWVQAMEGSAR

Target ORF information:

RefSeq Version	NM_133943.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133943.2

ORF Insert Sequence:

ATGGCAGACT CTGCACAGGT GCCAACACTG GTATACCTGG TCACAGGTGG CTGCGGCTTC 
CTGGGGGAAC ATATTGTTCG GATGCTGCTG GAACGGGAGC CCAGGCTCCG GGAGCTGCGT 
GTCTTTGACC TGCACCTGAG TTCTTGGCTG GAGGAGCTGA AAGCAGGGCC TGTGCAGGTG 
ACTGCCATCC AGGGGGATGT GACTCAGGCC CATGAGGTGG CAGCAGCCAT GTCTGGATCA 
CATGTGGTCA TCCATACAGC TGGGTTGGTG GATGTGTTTG GGAAGGCCAG TCCAAAGACC 
ATCCACAAAG TCAACGTGCA GGGCACACAG AATGTGATTG ATGCTTGTGT GCAGACTGGC 
ACTCAGTACC TGGTCTACAC GAGCAGCATG GAAGTGGTGG GGCCTAACAT CAAGGGCCAC 
CCCTTCTACA GGGGCAATGA AGATACCCCA TATGAGGCAG TCCACAGCCA TCCCTACCCA 
TGCAGTAAAG CCCTTGCTGA GCAGCTGGTC CTCGAGGCCA ATGGAAGGAA GGTCAATGGA 
GGGCTACCCC TGGTGACATG TGCCCTTCGA CCCACGGGCA TTTATGGTGA AGGTCATCAG 
GTCATGAGAG ACTTCTACTA CCAGGGACTG CGCTTTGGAG GTCGTCTATT TCGGGCCGTC 
CCAGCTTCTG TGGAGCACGG TCGGGTCTAT GTTGGCAATG TTGCTTGGAT GCACATACTG 
GTGGCCCGGG AGCTGGAGCA GCGGGCAGCA CTCATGGGTG GCCAGGTGTA TTTCTGCTAT 
GATAAGTCAC CTTATAAAAG CTACGAGGAC TTCAACATGG AGTTTCTGAG TCCCTGTGGT 
CTTCGACTGA TAGGCGCCCA CCCACTGCTG CCCTACTGGC TGCTAGTGCT GCTGGCTACC 
CTCAATGCCC TGCTGCAGTG GCTGCTCCGC CCACTGGTGC TGTACACACC CCTGCTGAAT 
CCCTACACGC TGGCTATGGC CAACACCACC TTTACTGTCA GTACCAACAA GGCACAGCGG 
CATTTTGGCT ACAAGCCCCT CTTCTCATGG GAAGAGAGCA GGACCCGCAC CATTCAGTGG 
GTGCAGGCGA TGGAGGGTTC AGCTCGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hsd3b7 ( XM_017321905.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177394.1 Mus musculus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu81883
Clone ID Related Accession (Same CDS sequence)	XM_017321905.1 , XM_017321906.1
Accession Version	XM_017321905.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	3 beta-hydroxysteroid dehydrogenase type 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAT CACATGTGGT CATCCATACA GCTGGGTTGG TGGATGTGTT TGGGAAGGCC 
AGTCCAAAGA CCATCCACAA AGTCAACGTG CAGGGCACAC AGAATGTGAT TGATGCTTGT 
GTGCAGACTG GCACTCAGTA CCTGGTCTAC ACGAGCAGCA TGGAAGTGGT GGGGCCTAAC 
ATCAAGGGCC ACCCCTTCTA CAGGGGCAAT GAAGATACCC CATATGAGGC AGTCCACAGC 
CATCCCTACC CATGCAGTAA AGCCCTTGCT GAGCAGCTGG TCCTCGAGGC CAATGGAAGG 
AAGGTCAATG GAGGGCTACC CCTGGTGACA TGTGCCCTTC GACCCACGGG CATTTATGGT 
GAAGGTCATC AGGTCATGAG AGACTTCTAC TACCAGGGAC TGCGCTTTGG AGGTCGTCTA 
TTTCGGGCCG TCCCAGCTTC TGTGGAGCAC GGTCGGGTCT ATGTTGGCAA TGTTGCTTGG 
ATGCACATAC TGGTGGCCCG GGAGCTGGAG CAGCGGGCAG CACTCATGGG TGGCCAGGTG 
TATTTCTGCT ATGATAAGTC ACCTTATAAA AGCTACGAGG ACTTCAACAT GGAGTTTCTG 
AGTCCCTGTG GTCTTCGACT GATAGGCGCC CACCCACTGC TGCCCTACTG GCTGCTAGTG 
CTGCTGGCTA CCCTCAATGC CCTGCTGCAG TGGCTGCTCC GCCCACTGGT GCTGTACACA 
CCCCTGCTGA ATCCCTACAC GCTGGCTATG GCCAACACCA CCTTTACTGT CAGTACCAAC 
AAGGCACAGC GGCATTTTGG CTACAAGCCC CTCTTCTCAT GGGAAGAGAG CAGGACCCGC 
ACCATTCAGT GGGTGCAGGC GATGGAGGGT TCAGCTCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177394.1
CDS	466..1347
Translation	MSGSHVVIHTAGLVDVFGKASPKTIHKVNVQGTQNVIDACVQTGTQYLVYTSSMEVVGPNIKGHPFYRGNEDTPYEAVHSHPYPCSKALAEQLVLEANGRKVNGGLPLVTCALRPTGIYGEGHQVMRDFYYQGLRFGGRLFRAVPASVEHGRVYVGNVAWMHILVARELEQRAALMGGQVYFCYDKSPYKSYEDFNMEFLSPCGLRLIGAHPLLPYWLLVLLATLNALLQWLLRPLVLYTPLLNPYTLAMANTTFTVSTNKAQRHFGYKPLFSWEESRTRTIQWVQAMEGSAR

Target ORF information:

RefSeq Version	XM_017321905.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321905.1

ORF Insert Sequence:

ATGTCTGGAT CACATGTGGT CATCCATACA GCTGGGTTGG TGGATGTGTT TGGGAAGGCC 
AGTCCAAAGA CCATCCACAA AGTCAACGTG CAGGGCACAC AGAATGTGAT TGATGCTTGT 
GTGCAGACTG GCACTCAGTA CCTGGTCTAC ACGAGCAGCA TGGAAGTGGT GGGGCCTAAC 
ATCAAGGGCC ACCCCTTCTA CAGGGGCAAT GAAGATACCC CATATGAGGC AGTCCACAGC 
CATCCCTACC CATGCAGTAA AGCCCTTGCT GAGCAGCTGG TCCTCGAGGC CAATGGAAGG 
AAGGTCAATG GAGGGCTACC CCTGGTGACA TGTGCCCTTC GACCCACGGG CATTTATGGT 
GAAGGTCATC AGGTCATGAG AGACTTCTAC TACCAGGGAC TGCGCTTTGG AGGTCGTCTA 
TTTCGGGCCG TCCCAGCTTC TGTGGAGCAC GGTCGGGTCT ATGTTGGCAA TGTTGCTTGG 
ATGCACATAC TGGTGGCCCG GGAGCTGGAG CAGCGGGCAG CACTCATGGG TGGCCAGGTG 
TATTTCTGCT ATGATAAGTC ACCTTATAAA AGCTACGAGG ACTTCAACAT GGAGTTTCTG 
AGTCCCTGTG GTCTTCGACT GATAGGCGCC CACCCACTGC TGCCCTACTG GCTGCTAGTG 
CTGCTGGCTA CCCTCAATGC CCTGCTGCAG TGGCTGCTCC GCCCACTGGT GCTGTACACA 
CCCCTGCTGA ATCCCTACAC GCTGGCTATG GCCAACACCA CCTTTACTGT CAGTACCAAC 
AAGGCACAGC GGCATTTTGG CTACAAGCCC CTCTTCTCAT GGGAAGAGAG CAGGACCCGC 
ACCATTCAGT GGGTGCAGGC GATGGAGGGT TCAGCTCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hsd3b7 ( XM_017321906.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017177395.1 Mus musculus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu81883
Clone ID Related Accession (Same CDS sequence)	XM_017321905.1 , XM_017321906.1
Accession Version	XM_017321906.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	3 beta-hydroxysteroid dehydrogenase type 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAT CACATGTGGT CATCCATACA GCTGGGTTGG TGGATGTGTT TGGGAAGGCC 
AGTCCAAAGA CCATCCACAA AGTCAACGTG CAGGGCACAC AGAATGTGAT TGATGCTTGT 
GTGCAGACTG GCACTCAGTA CCTGGTCTAC ACGAGCAGCA TGGAAGTGGT GGGGCCTAAC 
ATCAAGGGCC ACCCCTTCTA CAGGGGCAAT GAAGATACCC CATATGAGGC AGTCCACAGC 
CATCCCTACC CATGCAGTAA AGCCCTTGCT GAGCAGCTGG TCCTCGAGGC CAATGGAAGG 
AAGGTCAATG GAGGGCTACC CCTGGTGACA TGTGCCCTTC GACCCACGGG CATTTATGGT 
GAAGGTCATC AGGTCATGAG AGACTTCTAC TACCAGGGAC TGCGCTTTGG AGGTCGTCTA 
TTTCGGGCCG TCCCAGCTTC TGTGGAGCAC GGTCGGGTCT ATGTTGGCAA TGTTGCTTGG 
ATGCACATAC TGGTGGCCCG GGAGCTGGAG CAGCGGGCAG CACTCATGGG TGGCCAGGTG 
TATTTCTGCT ATGATAAGTC ACCTTATAAA AGCTACGAGG ACTTCAACAT GGAGTTTCTG 
AGTCCCTGTG GTCTTCGACT GATAGGCGCC CACCCACTGC TGCCCTACTG GCTGCTAGTG 
CTGCTGGCTA CCCTCAATGC CCTGCTGCAG TGGCTGCTCC GCCCACTGGT GCTGTACACA 
CCCCTGCTGA ATCCCTACAC GCTGGCTATG GCCAACACCA CCTTTACTGT CAGTACCAAC 
AAGGCACAGC GGCATTTTGG CTACAAGCCC CTCTTCTCAT GGGAAGAGAG CAGGACCCGC 
ACCATTCAGT GGGTGCAGGC GATGGAGGGT TCAGCTCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017177395.1
CDS	418..1299
Translation	MSGSHVVIHTAGLVDVFGKASPKTIHKVNVQGTQNVIDACVQTGTQYLVYTSSMEVVGPNIKGHPFYRGNEDTPYEAVHSHPYPCSKALAEQLVLEANGRKVNGGLPLVTCALRPTGIYGEGHQVMRDFYYQGLRFGGRLFRAVPASVEHGRVYVGNVAWMHILVARELEQRAALMGGQVYFCYDKSPYKSYEDFNMEFLSPCGLRLIGAHPLLPYWLLVLLATLNALLQWLLRPLVLYTPLLNPYTLAMANTTFTVSTNKAQRHFGYKPLFSWEESRTRTIQWVQAMEGSAR

Target ORF information:

RefSeq Version	XM_017321906.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7 (Hsd3b7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017321906.1

ORF Insert Sequence:

ATGTCTGGAT CACATGTGGT CATCCATACA GCTGGGTTGG TGGATGTGTT TGGGAAGGCC 
AGTCCAAAGA CCATCCACAA AGTCAACGTG CAGGGCACAC AGAATGTGAT TGATGCTTGT 
GTGCAGACTG GCACTCAGTA CCTGGTCTAC ACGAGCAGCA TGGAAGTGGT GGGGCCTAAC 
ATCAAGGGCC ACCCCTTCTA CAGGGGCAAT GAAGATACCC CATATGAGGC AGTCCACAGC 
CATCCCTACC CATGCAGTAA AGCCCTTGCT GAGCAGCTGG TCCTCGAGGC CAATGGAAGG 
AAGGTCAATG GAGGGCTACC CCTGGTGACA TGTGCCCTTC GACCCACGGG CATTTATGGT 
GAAGGTCATC AGGTCATGAG AGACTTCTAC TACCAGGGAC TGCGCTTTGG AGGTCGTCTA 
TTTCGGGCCG TCCCAGCTTC TGTGGAGCAC GGTCGGGTCT ATGTTGGCAA TGTTGCTTGG 
ATGCACATAC TGGTGGCCCG GGAGCTGGAG CAGCGGGCAG CACTCATGGG TGGCCAGGTG 
TATTTCTGCT ATGATAAGTC ACCTTATAAA AGCTACGAGG ACTTCAACAT GGAGTTTCTG 
AGTCCCTGTG GTCTTCGACT GATAGGCGCC CACCCACTGC TGCCCTACTG GCTGCTAGTG 
CTGCTGGCTA CCCTCAATGC CCTGCTGCAG TGGCTGCTCC GCCCACTGGT GCTGTACACA 
CCCCTGCTGA ATCCCTACAC GCTGGCTATG GCCAACACCA CCTTTACTGT CAGTACCAAC 
AAGGCACAGC GGCATTTTGG CTACAAGCCC CTCTTCTCAT GGGAAGAGAG CAGGACCCGC 
ACCATTCAGT GGGTGCAGGC GATGGAGGGT TCAGCTCGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

EBI2 augments Tfh cell fate by promoting interaction with IL-2-quenching dendritic cells.
Nature533(7601)110-4(2016 May)
Li J,Lu E,Yi T,Cyster JG

EBI2-mediated bridging channel positioning supports splenic dendritic cell homeostasis and particulate antigen capture.
eLife2e00757(2013 May)
Yi T,Cyster JG

Oxysterol gradient generation by lymphoid stromal cells guides activated B cell movement during humoral responses.
Immunity37(3)535-48(2012 Sep)
Yi T,Wang X,Kelly LM,An J,Xu Y,Sailer AW,Gustafsson JA,Russell DW,Cyster JG

Analysis of HSD3B7 knockout mice reveals that a 3alpha-hydroxyl stereochemistry is required for bile acid function.
Proceedings of the National Academy of Sciences of the United States of America104(28)11526-33(2007 Jul)
Shea HC,Head DD,Setchell KD,Russell DW

The bile acid synthetic gene 3beta-hydroxy-Delta(5)-C(27)-steroid oxidoreductase is mutated in progressive intrahepatic cholestasis.
The Journal of clinical investigation106(9)1175-84(2000 Nov)
Schwarz M,Wright AC,Davis DL,Nazer H,Bj?rkhem I,Russell DW

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Hsd3b7 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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