hsd3b7 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	hsd3b7
Entrez Gene ID	327462
Full Name	hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase
Synonyms	wu:fc37e10,wu:fi12g10,zgc:55799
General protein information	Preferred Names hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase Names 3 beta-hydroxysteroid dehydrogenase type 7 fc37e10 hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 7
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017353437.1	XP_017208926.1	3 beta-hydroxysteroid dehydrogenase type 7 isoform X1
XM_009299519.2	XP_009297794.1	3 beta-hydroxysteroid dehydrogenase type 7 isoform X1
XM_009299519.3	XP_009297794.1	3 beta-hydroxysteroid dehydrogenase type 7 isoform X1
XM_009299518.2	XP_009297793.1	3 beta-hydroxysteroid dehydrogenase type 7 isoform X1
XM_009299518.3	XP_009297793.1	3 beta-hydroxysteroid dehydrogenase type 7 isoform X1
XM_009299520.2	XP_009297795.1	3 beta-hydroxysteroid dehydrogenase type 7 isoform X1
NM_199809.1	NP_956103.1	3 beta-hydroxysteroid dehydrogenase type 7
XM_017353437.2	XP_017208926.1	3 beta-hydroxysteroid dehydrogenase type 7 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	HSD3B7	NP_079469.2
Rattus norvegicus (Norway rat)	Hsd3b7	NP_647545.1
Xenopus tropicalis (tropical clawed frog)	hsd3b7	NP_001120501.1
Bos taurus (cattle)	HSD3B7	NP_001029868.1
Danio rerio (zebrafish)	hsd3b7	NP_956103.1
Pan troglodytes (chimpanzee)	HSD3B7	XP_001155219.1
Canis lupus familiaris (dog)	HSD3B7	XP_547037.1
Mus musculus (house mouse)	Hsd3b7	NP_598704.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Protein Arginine Methyltransferase 6 (Prmt6) Is Essential for Early Zebrafish Development through the Direct Suppression of gadd45?a Stress Sensor Gene.
Zhao XX, Zhang YB, Ni PL, Wu ZL, Yan YC, Li YP
The Journal of biological chemistry291(1)402-12(2016 Jan)

Identification of differentially expressed genes in the zebrafish hypothalamic-pituitary axis.
Toro S, Wegner J, Muller M, Westerfield M, Varga ZM
Gene expression patterns : GEP9(4)200-8(2009 Apr)

Hepatic gene expression profiling using GeneChips in zebrafish exposed to 17alpha-methyldihydrotestosterone.
Hoffmann JL, Thomason RG, Lee DM, Brill JL, Price BB, Carr GJ, Versteeg DJ
Aquatic toxicology (Amsterdam, Netherlands)87(2)69-80(2008 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following hsd3b7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hsd3b7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa33147	NM_199809.1 Latest version!	Danio rerio hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase (hsd3b7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa39918	XM_009299518.3 Latest version!	Danio rerio hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase (hsd3b7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa39918	XM_009299520.2 Latest version!	Danio rerio hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase (hsd3b7), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa39918	XM_009299519.3 Latest version!	Danio rerio hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase (hsd3b7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa39918	XM_017353437.2 Latest version!	Danio rerio hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase (hsd3b7), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
ODa39918	XM_009299519.2	Danio rerio hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase (hsd3b7), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa39918	XM_009299518.2	Danio rerio hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase (hsd3b7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa39918	XM_017353437.1	Danio rerio hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase (hsd3b7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hsd3b7 ( NM_199809.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956103.1 Danio rerio hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase (hsd3b7), mRNA.

Price & Availability↑

CloneID	ODa33147
Clone ID Related Accession (Same CDS sequence)	NM_199809.1
Accession Version	NM_199809.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-18
Organism	Danio rerio(zebrafish)
Product	3 beta-hydroxysteroid dehydrogenase type 7
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC045457.1. ##Evidence-Data-START## Transcript exon combination :: BC045457.1, EE306698.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCCAACA ACAACAAGTC GAAGTTAACT TATGTCATAA CTGGAGGATG CGGTTTTCTG 
GGGCAGCACC TGTTGAGGGT TTTGCTGGAG AAGAAAAAAA ATGTCAAGGA GATCAGGCTT 
TTCGACAAAA ACGTTTTCCC AAGTCTTCAG AGTGAGAGTA CAGAGGATGT GAAAGTGGTC 
ATCATACAGG GAGATATAAC AAAATATGAG GATGTACGCA ATGCATTCCT GGGAGCAGAC 
CTGGTTTTCC ATGCCGCAAG TTTGGTTGAT GTGTGGTATA AAATTCCAGA GAAGGTCATC 
TTTGCTGTCA ATGTACAAGG GACGGAGAAT GCTATAAAAG CCTGTGTAGA AATCGGTATT 
CAGTATTTGG TCTACACAAG CTCCATGGAG GTGGTTGGTC CAAATGTAAA GGGAGATGAA 
TTTGTCAGAG GGAATGAGGA TACTCCATAT AATATATTCC ATGAGATGCC CTACCCTAAA 
TCCAAAGCTG CAGCTGAGAA AATTGTGCTG GAAGCCAATG GCACAAAGGT CGAGGGAGGG 
AACATTCTCT ACACATGCTG TCTGCGGCCA ACAGGAATCT ATGGGGAACA GCATCAGCTG 
ATGAAGGACT TCTATCTGAA CAGTGTCCGT AATGGCGGTT GGGTGATGAG AGGAGTTCCT 
CCTCATACGG AGCATGGACG CGTCTATGCA GGTAATGTGG CGTGGATGCA TTTACTAGCA 
GCACGTGCTC TGCAGGAACA TCCCAATCGG CTGGGTGGCG AATGTTATTT CTGCTATGAT 
GACTCGCCAT ACAAGCCCTA CGATGAATTC AACATGCAGT TTCTGTCAGC CTTCAACTTT 
CGCTCGCTTC GCCTGCCAGT GTGGATGCTG TGGATCATAG CATGGATGAA CGACATGGTC 
CGCTGGGTCC TGAAGCCCAT CTACAACTAC ACACCACTGC TCAACAAATA CACACTGGCT 
GTGGCCTGCA CATCCTTCAC CGTCAGCACA GACAAGGCTT TTCGGCACTT TCAGTACCAG 
CCGCTCTACA GCTGGCAGCA GTGCCTGTCT CGTACGCAGA GCTGGGTCAA CACTTTCCCA 
TTTGAAACCA GCACTAAAGA CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956103.1
CDS	78..1184
Translation	MSNNNKSKLTYVITGGCGFLGQHLLRVLLEKKKNVKEIRLFDKNVFPSLQSESTEDVKVVIIQGDITKYEDVRNAFLGADLVFHAASLVDVWYKIPEKVIFAVNVQGTENAIKACVEIGIQYLVYTSSMEVVGPNVKGDEFVRGNEDTPYNIFHEMPYPKSKAAAEKIVLEANGTKVEGGNILYTCCLRPTGIYGEQHQLMKDFYLNSVRNGGWVMRGVPPHTEHGRVYAGNVAWMHLLAARALQEHPNRLGGECYFCYDDSPYKPYDEFNMQFLSAFNFRSLRLPVWMLWIIAWMNDMVRWVLKPIYNYTPLLNKYTLAVACTSFTVSTDKAFRHFQYQPLYSWQQCLSRTQSWVNTFPFETSTKDK

Target ORF information:

RefSeq Version	NM_199809.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase (hsd3b7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199809.1

ORF Insert Sequence:

ATGTCCAACA ACAACAAGTC GAAGTTAACT TATGTCATAA CTGGAGGATG CGGTTTTCTG 
GGGCAGCACC TGTTGAGGGT TTTGCTGGAG AAGAAAAAAA ATGTCAAGGA GATCAGGCTT 
TTCGACAAAA ACGTTTTCCC AAGTCTTCAG AGTGAGAGTA CAGAGGATGT GAAAGTGGTC 
ATCATACAGG GAGATATAAC AAAATATGAG GATGTACGCA ATGCATTCCT GGGAGCAGAC 
CTGGTTTTCC ATGCCGCAAG TTTGGTTGAT GTGTGGTATA AAATTCCAGA GAAGGTCATC 
TTTGCTGTCA ATGTACAAGG GACGGAGAAT GCTATAAAAG CCTGTGTAGA AATCGGTATT 
CAGTATTTGG TCTACACAAG CTCCATGGAG GTGGTTGGTC CAAATGTAAA GGGAGATGAA 
TTTGTCAGAG GGAATGAGGA TACTCCATAT AATATATTCC ATGAGATGCC CTACCCTAAA 
TCCAAAGCTG CAGCTGAGAA AATTGTGCTG GAAGCCAATG GCACAAAGGT CGAGGGAGGG 
AACATTCTCT ACACATGCTG TCTGCGGCCA ACAGGAATCT ATGGGGAACA GCATCAGCTG 
ATGAAGGACT TCTATCTGAA CAGTGTCCGT AATGGCGGTT GGGTGATGAG AGGAGTTCCT 
CCTCATACGG AGCATGGACG CGTCTATGCA GGTAATGTGG CGTGGATGCA TTTACTAGCA 
GCACGTGCTC TGCAGGAACA TCCCAATCGG CTGGGTGGCG AATGTTATTT CTGCTATGAT 
GACTCGCCAT ACAAGCCCTA CGATGAATTC AACATGCAGT TTCTGTCAGC CTTCAACTTT 
CGCTCGCTTC GCCTGCCAGT GTGGATGCTG TGGATCATAG CATGGATGAA CGACATGGTC 
CGCTGGGTCC TGAAGCCCAT CTACAACTAC ACACCACTGC TCAACAAATA CACACTGGCT 
GTGGCCTGCA CATCCTTCAC CGTCAGCACA GACAAGGCTT TTCGGCACTT TCAGTACCAG 
CCGCTCTACA GCTGGCAGCA GTGCCTGTCT CGTACGCAGA GCTGGGTCAA CACTTTCCCA 
TTTGAAACCA GCACTAAAGA CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hsd3b7 ( XM_017353437.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208926.1 Danio rerio hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase (hsd3b7), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa39918
Clone ID Related Accession (Same CDS sequence)	XM_017353437.1 , XM_009299519.2 , XM_009299519.3 , XM_009299518.2 , XM_009299518.3 , XM_009299520.2 , XM_017353437.2
Accession Version	XM_017353437.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	3 beta-hydroxysteroid dehydrogenase type 7 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACA ACAACAAGTC GAAGTTAACT TATGTCATAA CTGGAGGATG CGGTTTTCTG 
GGGCAGCACC TGTTGAGGGT TTTGCTGGAG AAGGAAAAAA ATGTCAAGGA GATCAGGCTT 
TTCGACAAAA ACGTTTTCCC AAGTCTTCAG AGTGAGAGTA CAGAGGATGT GAAAGTGGTC 
ATCATACAGG GAGATATAAC AAAATATGAG GATGTACGCA ATGCATTCCT GGGAGCAGAC 
CTGGTTTTCC ATGCCGCAAG TTTGGTTGAT GTGTGGTATA AAATTCCAGA GAAGGTCATC 
TTTGCTGTCA ATGTACAAGG GACGGAGAAT GCTATAAAAG CCTGTGTAGA AATCGGTATT 
CAGTATTTGG TCTACACAAG CTCCATGGAG GTGGTTGGTC CAAATGTAAA GGGAGATGAA 
TTTGTCAGAG GGAATGAGGA TACTCCATAT AATATATTCC ATGAGATGCC CTACCCTAAA 
TCCAAAGCTG CAGCTGAGAA AATTGTGCTG GAAGCCAATG GCACAAAGGT CGAGGGAGGG 
AACATTCTCT ACACATGCTG TCTGCGGCCA ACAGGAATCT ATGGGGAACA GCATCAGCTG 
ATGAAGGACT TCTATCTGAA CAGTGTCCGT AATGGCGGTT GGGTGATGAG AGGAGTTCCT 
CCTCATACGG AGCATGGACG CGTCTATGCA GGTAATGTGG CGTGGATGCA TTTACTAGCA 
GCACGTGCTC TGCAGGAACA TCCCAATCGG CTGGGTGGCG AATGTTATTT CTGCTATGAT 
GACTCGCCAT ACAAGCCCTA CGATGAATTC AACATGCAGT TTCTGTCAGC CTTCAACTTT 
CGCTCGCTTC GCCTGCCAGT GTGGATGCTG TGGATCATAG CATGGATGAA CGACATGGTC 
CGCTGGGTCC TGAAGCCCAT CTACAACTAC ACACCACTGC TCAACAAATA CACACTGGCT 
GTGGCCTGCA CATCCTTCAC CGTCAGCACA GACAAGGCTT TTCGGCACTT TCAGTACCAG 
CCGCTCTACA GCTGGCAGCA GTGCCTGTCT CGTACGCAGA GCTGGGTCAA CACTTTCCCA 
TTTGAAACCA GCACTAAAGA CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208926.1
CDS	274..1380
Translation	MSNNNKSKLTYVITGGCGFLGQHLLRVLLEKEKNVKEIRLFDKNVFPSLQSESTEDVKVVIIQGDITKYEDVRNAFLGADLVFHAASLVDVWYKIPEKVIFAVNVQGTENAIKACVEIGIQYLVYTSSMEVVGPNVKGDEFVRGNEDTPYNIFHEMPYPKSKAAAEKIVLEANGTKVEGGNILYTCCLRPTGIYGEQHQLMKDFYLNSVRNGGWVMRGVPPHTEHGRVYAGNVAWMHLLAARALQEHPNRLGGECYFCYDDSPYKPYDEFNMQFLSAFNFRSLRLPVWMLWIIAWMNDMVRWVLKPIYNYTPLLNKYTLAVACTSFTVSTDKAFRHFQYQPLYSWQQCLSRTQSWVNTFPFETSTKDK

Target ORF information:

RefSeq Version	XM_017353437.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase (hsd3b7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353437.1

ORF Insert Sequence:

ATGTCCAACA ACAACAAGTC GAAGTTAACT TATGTCATAA CTGGAGGATG CGGTTTTCTG 
GGGCAGCACC TGTTGAGGGT TTTGCTGGAG AAGGAAAAAA ATGTCAAGGA GATCAGGCTT 
TTCGACAAAA ACGTTTTCCC AAGTCTTCAG AGTGAGAGTA CAGAGGATGT GAAAGTGGTC 
ATCATACAGG GAGATATAAC AAAATATGAG GATGTACGCA ATGCATTCCT GGGAGCAGAC 
CTGGTTTTCC ATGCCGCAAG TTTGGTTGAT GTGTGGTATA AAATTCCAGA GAAGGTCATC 
TTTGCTGTCA ATGTACAAGG GACGGAGAAT GCTATAAAAG CCTGTGTAGA AATCGGTATT 
CAGTATTTGG TCTACACAAG CTCCATGGAG GTGGTTGGTC CAAATGTAAA GGGAGATGAA 
TTTGTCAGAG GGAATGAGGA TACTCCATAT AATATATTCC ATGAGATGCC CTACCCTAAA 
TCCAAAGCTG CAGCTGAGAA AATTGTGCTG GAAGCCAATG GCACAAAGGT CGAGGGAGGG 
AACATTCTCT ACACATGCTG TCTGCGGCCA ACAGGAATCT ATGGGGAACA GCATCAGCTG 
ATGAAGGACT TCTATCTGAA CAGTGTCCGT AATGGCGGTT GGGTGATGAG AGGAGTTCCT 
CCTCATACGG AGCATGGACG CGTCTATGCA GGTAATGTGG CGTGGATGCA TTTACTAGCA 
GCACGTGCTC TGCAGGAACA TCCCAATCGG CTGGGTGGCG AATGTTATTT CTGCTATGAT 
GACTCGCCAT ACAAGCCCTA CGATGAATTC AACATGCAGT TTCTGTCAGC CTTCAACTTT 
CGCTCGCTTC GCCTGCCAGT GTGGATGCTG TGGATCATAG CATGGATGAA CGACATGGTC 
CGCTGGGTCC TGAAGCCCAT CTACAACTAC ACACCACTGC TCAACAAATA CACACTGGCT 
GTGGCCTGCA CATCCTTCAC CGTCAGCACA GACAAGGCTT TTCGGCACTT TCAGTACCAG 
CCGCTCTACA GCTGGCAGCA GTGCCTGTCT CGTACGCAGA GCTGGGTCAA CACTTTCCCA 
TTTGAAACCA GCACTAAAGA CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hsd3b7 ( XM_009299519.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297794.1 Danio rerio hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase (hsd3b7), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa39918
Clone ID Related Accession (Same CDS sequence)	XM_017353437.1 , XM_009299519.2 , XM_009299519.3 , XM_009299518.2 , XM_009299518.3 , XM_009299520.2 , XM_017353437.2
Accession Version	XM_009299519.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	3 beta-hydroxysteroid dehydrogenase type 7 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299519.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACA ACAACAAGTC GAAGTTAACT TATGTCATAA CTGGAGGATG CGGTTTTCTG 
GGGCAGCACC TGTTGAGGGT TTTGCTGGAG AAGGAAAAAA ATGTCAAGGA GATCAGGCTT 
TTCGACAAAA ACGTTTTCCC AAGTCTTCAG AGTGAGAGTA CAGAGGATGT GAAAGTGGTC 
ATCATACAGG GAGATATAAC AAAATATGAG GATGTACGCA ATGCATTCCT GGGAGCAGAC 
CTGGTTTTCC ATGCCGCAAG TTTGGTTGAT GTGTGGTATA AAATTCCAGA GAAGGTCATC 
TTTGCTGTCA ATGTACAAGG GACGGAGAAT GCTATAAAAG CCTGTGTAGA AATCGGTATT 
CAGTATTTGG TCTACACAAG CTCCATGGAG GTGGTTGGTC CAAATGTAAA GGGAGATGAA 
TTTGTCAGAG GGAATGAGGA TACTCCATAT AATATATTCC ATGAGATGCC CTACCCTAAA 
TCCAAAGCTG CAGCTGAGAA AATTGTGCTG GAAGCCAATG GCACAAAGGT CGAGGGAGGG 
AACATTCTCT ACACATGCTG TCTGCGGCCA ACAGGAATCT ATGGGGAACA GCATCAGCTG 
ATGAAGGACT TCTATCTGAA CAGTGTCCGT AATGGCGGTT GGGTGATGAG AGGAGTTCCT 
CCTCATACGG AGCATGGACG CGTCTATGCA GGTAATGTGG CGTGGATGCA TTTACTAGCA 
GCACGTGCTC TGCAGGAACA TCCCAATCGG CTGGGTGGCG AATGTTATTT CTGCTATGAT 
GACTCGCCAT ACAAGCCCTA CGATGAATTC AACATGCAGT TTCTGTCAGC CTTCAACTTT 
CGCTCGCTTC GCCTGCCAGT GTGGATGCTG TGGATCATAG CATGGATGAA CGACATGGTC 
CGCTGGGTCC TGAAGCCCAT CTACAACTAC ACACCACTGC TCAACAAATA CACACTGGCT 
GTGGCCTGCA CATCCTTCAC CGTCAGCACA GACAAGGCTT TTCGGCACTT TCAGTACCAG 
CCGCTCTACA GCTGGCAGCA GTGCCTGTCT CGTACGCAGA GCTGGGTCAA CACTTTCCCA 
TTTGAAACCA GCACTAAAGA CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297794.1
CDS	242..1348
Translation	MSNNNKSKLTYVITGGCGFLGQHLLRVLLEKEKNVKEIRLFDKNVFPSLQSESTEDVKVVIIQGDITKYEDVRNAFLGADLVFHAASLVDVWYKIPEKVIFAVNVQGTENAIKACVEIGIQYLVYTSSMEVVGPNVKGDEFVRGNEDTPYNIFHEMPYPKSKAAAEKIVLEANGTKVEGGNILYTCCLRPTGIYGEQHQLMKDFYLNSVRNGGWVMRGVPPHTEHGRVYAGNVAWMHLLAARALQEHPNRLGGECYFCYDDSPYKPYDEFNMQFLSAFNFRSLRLPVWMLWIIAWMNDMVRWVLKPIYNYTPLLNKYTLAVACTSFTVSTDKAFRHFQYQPLYSWQQCLSRTQSWVNTFPFETSTKDK

Target ORF information:

RefSeq Version	XM_009299519.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase (hsd3b7), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299519.2

ORF Insert Sequence:

ATGTCCAACA ACAACAAGTC GAAGTTAACT TATGTCATAA CTGGAGGATG CGGTTTTCTG 
GGGCAGCACC TGTTGAGGGT TTTGCTGGAG AAGGAAAAAA ATGTCAAGGA GATCAGGCTT 
TTCGACAAAA ACGTTTTCCC AAGTCTTCAG AGTGAGAGTA CAGAGGATGT GAAAGTGGTC 
ATCATACAGG GAGATATAAC AAAATATGAG GATGTACGCA ATGCATTCCT GGGAGCAGAC 
CTGGTTTTCC ATGCCGCAAG TTTGGTTGAT GTGTGGTATA AAATTCCAGA GAAGGTCATC 
TTTGCTGTCA ATGTACAAGG GACGGAGAAT GCTATAAAAG CCTGTGTAGA AATCGGTATT 
CAGTATTTGG TCTACACAAG CTCCATGGAG GTGGTTGGTC CAAATGTAAA GGGAGATGAA 
TTTGTCAGAG GGAATGAGGA TACTCCATAT AATATATTCC ATGAGATGCC CTACCCTAAA 
TCCAAAGCTG CAGCTGAGAA AATTGTGCTG GAAGCCAATG GCACAAAGGT CGAGGGAGGG 
AACATTCTCT ACACATGCTG TCTGCGGCCA ACAGGAATCT ATGGGGAACA GCATCAGCTG 
ATGAAGGACT TCTATCTGAA CAGTGTCCGT AATGGCGGTT GGGTGATGAG AGGAGTTCCT 
CCTCATACGG AGCATGGACG CGTCTATGCA GGTAATGTGG CGTGGATGCA TTTACTAGCA 
GCACGTGCTC TGCAGGAACA TCCCAATCGG CTGGGTGGCG AATGTTATTT CTGCTATGAT 
GACTCGCCAT ACAAGCCCTA CGATGAATTC AACATGCAGT TTCTGTCAGC CTTCAACTTT 
CGCTCGCTTC GCCTGCCAGT GTGGATGCTG TGGATCATAG CATGGATGAA CGACATGGTC 
CGCTGGGTCC TGAAGCCCAT CTACAACTAC ACACCACTGC TCAACAAATA CACACTGGCT 
GTGGCCTGCA CATCCTTCAC CGTCAGCACA GACAAGGCTT TTCGGCACTT TCAGTACCAG 
CCGCTCTACA GCTGGCAGCA GTGCCTGTCT CGTACGCAGA GCTGGGTCAA CACTTTCCCA 
TTTGAAACCA GCACTAAAGA CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hsd3b7 ( XM_009299519.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297794.1 Danio rerio hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase (hsd3b7), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa39918
Clone ID Related Accession (Same CDS sequence)	XM_017353437.1 , XM_009299519.2 , XM_009299519.3 , XM_009299518.2 , XM_009299518.3 , XM_009299520.2 , XM_017353437.2
Accession Version	XM_009299519.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	3 beta-hydroxysteroid dehydrogenase type 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299519.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACA ACAACAAGTC GAAGTTAACT TATGTCATAA CTGGAGGATG CGGTTTTCTG 
GGGCAGCACC TGTTGAGGGT TTTGCTGGAG AAGGAAAAAA ATGTCAAGGA GATCAGGCTT 
TTCGACAAAA ACGTTTTCCC AAGTCTTCAG AGTGAGAGTA CAGAGGATGT GAAAGTGGTC 
ATCATACAGG GAGATATAAC AAAATATGAG GATGTACGCA ATGCATTCCT GGGAGCAGAC 
CTGGTTTTCC ATGCCGCAAG TTTGGTTGAT GTGTGGTATA AAATTCCAGA GAAGGTCATC 
TTTGCTGTCA ATGTACAAGG GACGGAGAAT GCTATAAAAG CCTGTGTAGA AATCGGTATT 
CAGTATTTGG TCTACACAAG CTCCATGGAG GTGGTTGGTC CAAATGTAAA GGGAGATGAA 
TTTGTCAGAG GGAATGAGGA TACTCCATAT AATATATTCC ATGAGATGCC CTACCCTAAA 
TCCAAAGCTG CAGCTGAGAA AATTGTGCTG GAAGCCAATG GCACAAAGGT CGAGGGAGGG 
AACATTCTCT ACACATGCTG TCTGCGGCCA ACAGGAATCT ATGGGGAACA GCATCAGCTG 
ATGAAGGACT TCTATCTGAA CAGTGTCCGT AATGGCGGTT GGGTGATGAG AGGAGTTCCT 
CCTCATACGG AGCATGGACG CGTCTATGCA GGTAATGTGG CGTGGATGCA TTTACTAGCA 
GCACGTGCTC TGCAGGAACA TCCCAATCGG CTGGGTGGCG AATGTTATTT CTGCTATGAT 
GACTCGCCAT ACAAGCCCTA CGATGAATTC AACATGCAGT TTCTGTCAGC CTTCAACTTT 
CGCTCGCTTC GCCTGCCAGT GTGGATGCTG TGGATCATAG CATGGATGAA CGACATGGTC 
CGCTGGGTCC TGAAGCCCAT CTACAACTAC ACACCACTGC TCAACAAATA CACACTGGCT 
GTGGCCTGCA CATCCTTCAC CGTCAGCACA GACAAGGCTT TTCGGCACTT TCAGTACCAG 
CCGCTCTACA GCTGGCAGCA GTGCCTGTCT CGTACGCAGA GCTGGGTCAA CACTTTCCCA 
TTTGAAACCA GCACTAAAGA CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297794.1
CDS	266..1372
Translation	MSNNNKSKLTYVITGGCGFLGQHLLRVLLEKEKNVKEIRLFDKNVFPSLQSESTEDVKVVIIQGDITKYEDVRNAFLGADLVFHAASLVDVWYKIPEKVIFAVNVQGTENAIKACVEIGIQYLVYTSSMEVVGPNVKGDEFVRGNEDTPYNIFHEMPYPKSKAAAEKIVLEANGTKVEGGNILYTCCLRPTGIYGEQHQLMKDFYLNSVRNGGWVMRGVPPHTEHGRVYAGNVAWMHLLAARALQEHPNRLGGECYFCYDDSPYKPYDEFNMQFLSAFNFRSLRLPVWMLWIIAWMNDMVRWVLKPIYNYTPLLNKYTLAVACTSFTVSTDKAFRHFQYQPLYSWQQCLSRTQSWVNTFPFETSTKDK

Target ORF information:

RefSeq Version	XM_009299519.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase (hsd3b7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299519.3

ORF Insert Sequence:

ATGTCCAACA ACAACAAGTC GAAGTTAACT TATGTCATAA CTGGAGGATG CGGTTTTCTG 
GGGCAGCACC TGTTGAGGGT TTTGCTGGAG AAGGAAAAAA ATGTCAAGGA GATCAGGCTT 
TTCGACAAAA ACGTTTTCCC AAGTCTTCAG AGTGAGAGTA CAGAGGATGT GAAAGTGGTC 
ATCATACAGG GAGATATAAC AAAATATGAG GATGTACGCA ATGCATTCCT GGGAGCAGAC 
CTGGTTTTCC ATGCCGCAAG TTTGGTTGAT GTGTGGTATA AAATTCCAGA GAAGGTCATC 
TTTGCTGTCA ATGTACAAGG GACGGAGAAT GCTATAAAAG CCTGTGTAGA AATCGGTATT 
CAGTATTTGG TCTACACAAG CTCCATGGAG GTGGTTGGTC CAAATGTAAA GGGAGATGAA 
TTTGTCAGAG GGAATGAGGA TACTCCATAT AATATATTCC ATGAGATGCC CTACCCTAAA 
TCCAAAGCTG CAGCTGAGAA AATTGTGCTG GAAGCCAATG GCACAAAGGT CGAGGGAGGG 
AACATTCTCT ACACATGCTG TCTGCGGCCA ACAGGAATCT ATGGGGAACA GCATCAGCTG 
ATGAAGGACT TCTATCTGAA CAGTGTCCGT AATGGCGGTT GGGTGATGAG AGGAGTTCCT 
CCTCATACGG AGCATGGACG CGTCTATGCA GGTAATGTGG CGTGGATGCA TTTACTAGCA 
GCACGTGCTC TGCAGGAACA TCCCAATCGG CTGGGTGGCG AATGTTATTT CTGCTATGAT 
GACTCGCCAT ACAAGCCCTA CGATGAATTC AACATGCAGT TTCTGTCAGC CTTCAACTTT 
CGCTCGCTTC GCCTGCCAGT GTGGATGCTG TGGATCATAG CATGGATGAA CGACATGGTC 
CGCTGGGTCC TGAAGCCCAT CTACAACTAC ACACCACTGC TCAACAAATA CACACTGGCT 
GTGGCCTGCA CATCCTTCAC CGTCAGCACA GACAAGGCTT TTCGGCACTT TCAGTACCAG 
CCGCTCTACA GCTGGCAGCA GTGCCTGTCT CGTACGCAGA GCTGGGTCAA CACTTTCCCA 
TTTGAAACCA GCACTAAAGA CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hsd3b7 ( XM_009299518.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297793.1 Danio rerio hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase (hsd3b7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa39918
Clone ID Related Accession (Same CDS sequence)	XM_017353437.1 , XM_009299519.2 , XM_009299519.3 , XM_009299518.2 , XM_009299518.3 , XM_009299520.2 , XM_017353437.2
Accession Version	XM_009299518.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	3 beta-hydroxysteroid dehydrogenase type 7 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299518.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACA ACAACAAGTC GAAGTTAACT TATGTCATAA CTGGAGGATG CGGTTTTCTG 
GGGCAGCACC TGTTGAGGGT TTTGCTGGAG AAGGAAAAAA ATGTCAAGGA GATCAGGCTT 
TTCGACAAAA ACGTTTTCCC AAGTCTTCAG AGTGAGAGTA CAGAGGATGT GAAAGTGGTC 
ATCATACAGG GAGATATAAC AAAATATGAG GATGTACGCA ATGCATTCCT GGGAGCAGAC 
CTGGTTTTCC ATGCCGCAAG TTTGGTTGAT GTGTGGTATA AAATTCCAGA GAAGGTCATC 
TTTGCTGTCA ATGTACAAGG GACGGAGAAT GCTATAAAAG CCTGTGTAGA AATCGGTATT 
CAGTATTTGG TCTACACAAG CTCCATGGAG GTGGTTGGTC CAAATGTAAA GGGAGATGAA 
TTTGTCAGAG GGAATGAGGA TACTCCATAT AATATATTCC ATGAGATGCC CTACCCTAAA 
TCCAAAGCTG CAGCTGAGAA AATTGTGCTG GAAGCCAATG GCACAAAGGT CGAGGGAGGG 
AACATTCTCT ACACATGCTG TCTGCGGCCA ACAGGAATCT ATGGGGAACA GCATCAGCTG 
ATGAAGGACT TCTATCTGAA CAGTGTCCGT AATGGCGGTT GGGTGATGAG AGGAGTTCCT 
CCTCATACGG AGCATGGACG CGTCTATGCA GGTAATGTGG CGTGGATGCA TTTACTAGCA 
GCACGTGCTC TGCAGGAACA TCCCAATCGG CTGGGTGGCG AATGTTATTT CTGCTATGAT 
GACTCGCCAT ACAAGCCCTA CGATGAATTC AACATGCAGT TTCTGTCAGC CTTCAACTTT 
CGCTCGCTTC GCCTGCCAGT GTGGATGCTG TGGATCATAG CATGGATGAA CGACATGGTC 
CGCTGGGTCC TGAAGCCCAT CTACAACTAC ACACCACTGC TCAACAAATA CACACTGGCT 
GTGGCCTGCA CATCCTTCAC CGTCAGCACA GACAAGGCTT TTCGGCACTT TCAGTACCAG 
CCGCTCTACA GCTGGCAGCA GTGCCTGTCT CGTACGCAGA GCTGGGTCAA CACTTTCCCA 
TTTGAAACCA GCACTAAAGA CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297793.1
CDS	364..1470
Translation	MSNNNKSKLTYVITGGCGFLGQHLLRVLLEKEKNVKEIRLFDKNVFPSLQSESTEDVKVVIIQGDITKYEDVRNAFLGADLVFHAASLVDVWYKIPEKVIFAVNVQGTENAIKACVEIGIQYLVYTSSMEVVGPNVKGDEFVRGNEDTPYNIFHEMPYPKSKAAAEKIVLEANGTKVEGGNILYTCCLRPTGIYGEQHQLMKDFYLNSVRNGGWVMRGVPPHTEHGRVYAGNVAWMHLLAARALQEHPNRLGGECYFCYDDSPYKPYDEFNMQFLSAFNFRSLRLPVWMLWIIAWMNDMVRWVLKPIYNYTPLLNKYTLAVACTSFTVSTDKAFRHFQYQPLYSWQQCLSRTQSWVNTFPFETSTKDK

Target ORF information:

RefSeq Version	XM_009299518.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase (hsd3b7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299518.2

ORF Insert Sequence:

ATGTCCAACA ACAACAAGTC GAAGTTAACT TATGTCATAA CTGGAGGATG CGGTTTTCTG 
GGGCAGCACC TGTTGAGGGT TTTGCTGGAG AAGGAAAAAA ATGTCAAGGA GATCAGGCTT 
TTCGACAAAA ACGTTTTCCC AAGTCTTCAG AGTGAGAGTA CAGAGGATGT GAAAGTGGTC 
ATCATACAGG GAGATATAAC AAAATATGAG GATGTACGCA ATGCATTCCT GGGAGCAGAC 
CTGGTTTTCC ATGCCGCAAG TTTGGTTGAT GTGTGGTATA AAATTCCAGA GAAGGTCATC 
TTTGCTGTCA ATGTACAAGG GACGGAGAAT GCTATAAAAG CCTGTGTAGA AATCGGTATT 
CAGTATTTGG TCTACACAAG CTCCATGGAG GTGGTTGGTC CAAATGTAAA GGGAGATGAA 
TTTGTCAGAG GGAATGAGGA TACTCCATAT AATATATTCC ATGAGATGCC CTACCCTAAA 
TCCAAAGCTG CAGCTGAGAA AATTGTGCTG GAAGCCAATG GCACAAAGGT CGAGGGAGGG 
AACATTCTCT ACACATGCTG TCTGCGGCCA ACAGGAATCT ATGGGGAACA GCATCAGCTG 
ATGAAGGACT TCTATCTGAA CAGTGTCCGT AATGGCGGTT GGGTGATGAG AGGAGTTCCT 
CCTCATACGG AGCATGGACG CGTCTATGCA GGTAATGTGG CGTGGATGCA TTTACTAGCA 
GCACGTGCTC TGCAGGAACA TCCCAATCGG CTGGGTGGCG AATGTTATTT CTGCTATGAT 
GACTCGCCAT ACAAGCCCTA CGATGAATTC AACATGCAGT TTCTGTCAGC CTTCAACTTT 
CGCTCGCTTC GCCTGCCAGT GTGGATGCTG TGGATCATAG CATGGATGAA CGACATGGTC 
CGCTGGGTCC TGAAGCCCAT CTACAACTAC ACACCACTGC TCAACAAATA CACACTGGCT 
GTGGCCTGCA CATCCTTCAC CGTCAGCACA GACAAGGCTT TTCGGCACTT TCAGTACCAG 
CCGCTCTACA GCTGGCAGCA GTGCCTGTCT CGTACGCAGA GCTGGGTCAA CACTTTCCCA 
TTTGAAACCA GCACTAAAGA CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hsd3b7 ( XM_009299518.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297793.1 Danio rerio hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase (hsd3b7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa39918
Clone ID Related Accession (Same CDS sequence)	XM_017353437.1 , XM_009299519.2 , XM_009299519.3 , XM_009299518.2 , XM_009299518.3 , XM_009299520.2 , XM_017353437.2
Accession Version	XM_009299518.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	3 beta-hydroxysteroid dehydrogenase type 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299518.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACA ACAACAAGTC GAAGTTAACT TATGTCATAA CTGGAGGATG CGGTTTTCTG 
GGGCAGCACC TGTTGAGGGT TTTGCTGGAG AAGGAAAAAA ATGTCAAGGA GATCAGGCTT 
TTCGACAAAA ACGTTTTCCC AAGTCTTCAG AGTGAGAGTA CAGAGGATGT GAAAGTGGTC 
ATCATACAGG GAGATATAAC AAAATATGAG GATGTACGCA ATGCATTCCT GGGAGCAGAC 
CTGGTTTTCC ATGCCGCAAG TTTGGTTGAT GTGTGGTATA AAATTCCAGA GAAGGTCATC 
TTTGCTGTCA ATGTACAAGG GACGGAGAAT GCTATAAAAG CCTGTGTAGA AATCGGTATT 
CAGTATTTGG TCTACACAAG CTCCATGGAG GTGGTTGGTC CAAATGTAAA GGGAGATGAA 
TTTGTCAGAG GGAATGAGGA TACTCCATAT AATATATTCC ATGAGATGCC CTACCCTAAA 
TCCAAAGCTG CAGCTGAGAA AATTGTGCTG GAAGCCAATG GCACAAAGGT CGAGGGAGGG 
AACATTCTCT ACACATGCTG TCTGCGGCCA ACAGGAATCT ATGGGGAACA GCATCAGCTG 
ATGAAGGACT TCTATCTGAA CAGTGTCCGT AATGGCGGTT GGGTGATGAG AGGAGTTCCT 
CCTCATACGG AGCATGGACG CGTCTATGCA GGTAATGTGG CGTGGATGCA TTTACTAGCA 
GCACGTGCTC TGCAGGAACA TCCCAATCGG CTGGGTGGCG AATGTTATTT CTGCTATGAT 
GACTCGCCAT ACAAGCCCTA CGATGAATTC AACATGCAGT TTCTGTCAGC CTTCAACTTT 
CGCTCGCTTC GCCTGCCAGT GTGGATGCTG TGGATCATAG CATGGATGAA CGACATGGTC 
CGCTGGGTCC TGAAGCCCAT CTACAACTAC ACACCACTGC TCAACAAATA CACACTGGCT 
GTGGCCTGCA CATCCTTCAC CGTCAGCACA GACAAGGCTT TTCGGCACTT TCAGTACCAG 
CCGCTCTACA GCTGGCAGCA GTGCCTGTCT CGTACGCAGA GCTGGGTCAA CACTTTCCCA 
TTTGAAACCA GCACTAAAGA CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297793.1
CDS	388..1494
Translation	MSNNNKSKLTYVITGGCGFLGQHLLRVLLEKEKNVKEIRLFDKNVFPSLQSESTEDVKVVIIQGDITKYEDVRNAFLGADLVFHAASLVDVWYKIPEKVIFAVNVQGTENAIKACVEIGIQYLVYTSSMEVVGPNVKGDEFVRGNEDTPYNIFHEMPYPKSKAAAEKIVLEANGTKVEGGNILYTCCLRPTGIYGEQHQLMKDFYLNSVRNGGWVMRGVPPHTEHGRVYAGNVAWMHLLAARALQEHPNRLGGECYFCYDDSPYKPYDEFNMQFLSAFNFRSLRLPVWMLWIIAWMNDMVRWVLKPIYNYTPLLNKYTLAVACTSFTVSTDKAFRHFQYQPLYSWQQCLSRTQSWVNTFPFETSTKDK

Target ORF information:

RefSeq Version	XM_009299518.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase (hsd3b7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299518.3

ORF Insert Sequence:

ATGTCCAACA ACAACAAGTC GAAGTTAACT TATGTCATAA CTGGAGGATG CGGTTTTCTG 
GGGCAGCACC TGTTGAGGGT TTTGCTGGAG AAGGAAAAAA ATGTCAAGGA GATCAGGCTT 
TTCGACAAAA ACGTTTTCCC AAGTCTTCAG AGTGAGAGTA CAGAGGATGT GAAAGTGGTC 
ATCATACAGG GAGATATAAC AAAATATGAG GATGTACGCA ATGCATTCCT GGGAGCAGAC 
CTGGTTTTCC ATGCCGCAAG TTTGGTTGAT GTGTGGTATA AAATTCCAGA GAAGGTCATC 
TTTGCTGTCA ATGTACAAGG GACGGAGAAT GCTATAAAAG CCTGTGTAGA AATCGGTATT 
CAGTATTTGG TCTACACAAG CTCCATGGAG GTGGTTGGTC CAAATGTAAA GGGAGATGAA 
TTTGTCAGAG GGAATGAGGA TACTCCATAT AATATATTCC ATGAGATGCC CTACCCTAAA 
TCCAAAGCTG CAGCTGAGAA AATTGTGCTG GAAGCCAATG GCACAAAGGT CGAGGGAGGG 
AACATTCTCT ACACATGCTG TCTGCGGCCA ACAGGAATCT ATGGGGAACA GCATCAGCTG 
ATGAAGGACT TCTATCTGAA CAGTGTCCGT AATGGCGGTT GGGTGATGAG AGGAGTTCCT 
CCTCATACGG AGCATGGACG CGTCTATGCA GGTAATGTGG CGTGGATGCA TTTACTAGCA 
GCACGTGCTC TGCAGGAACA TCCCAATCGG CTGGGTGGCG AATGTTATTT CTGCTATGAT 
GACTCGCCAT ACAAGCCCTA CGATGAATTC AACATGCAGT TTCTGTCAGC CTTCAACTTT 
CGCTCGCTTC GCCTGCCAGT GTGGATGCTG TGGATCATAG CATGGATGAA CGACATGGTC 
CGCTGGGTCC TGAAGCCCAT CTACAACTAC ACACCACTGC TCAACAAATA CACACTGGCT 
GTGGCCTGCA CATCCTTCAC CGTCAGCACA GACAAGGCTT TTCGGCACTT TCAGTACCAG 
CCGCTCTACA GCTGGCAGCA GTGCCTGTCT CGTACGCAGA GCTGGGTCAA CACTTTCCCA 
TTTGAAACCA GCACTAAAGA CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hsd3b7 ( XM_009299520.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009297795.1 Danio rerio hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase (hsd3b7), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa39918
Clone ID Related Accession (Same CDS sequence)	XM_017353437.1 , XM_009299519.2 , XM_009299519.3 , XM_009299518.2 , XM_009299518.3 , XM_009299520.2 , XM_017353437.2
Accession Version	XM_009299520.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	3 beta-hydroxysteroid dehydrogenase type 7 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299520.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACA ACAACAAGTC GAAGTTAACT TATGTCATAA CTGGAGGATG CGGTTTTCTG 
GGGCAGCACC TGTTGAGGGT TTTGCTGGAG AAGGAAAAAA ATGTCAAGGA GATCAGGCTT 
TTCGACAAAA ACGTTTTCCC AAGTCTTCAG AGTGAGAGTA CAGAGGATGT GAAAGTGGTC 
ATCATACAGG GAGATATAAC AAAATATGAG GATGTACGCA ATGCATTCCT GGGAGCAGAC 
CTGGTTTTCC ATGCCGCAAG TTTGGTTGAT GTGTGGTATA AAATTCCAGA GAAGGTCATC 
TTTGCTGTCA ATGTACAAGG GACGGAGAAT GCTATAAAAG CCTGTGTAGA AATCGGTATT 
CAGTATTTGG TCTACACAAG CTCCATGGAG GTGGTTGGTC CAAATGTAAA GGGAGATGAA 
TTTGTCAGAG GGAATGAGGA TACTCCATAT AATATATTCC ATGAGATGCC CTACCCTAAA 
TCCAAAGCTG CAGCTGAGAA AATTGTGCTG GAAGCCAATG GCACAAAGGT CGAGGGAGGG 
AACATTCTCT ACACATGCTG TCTGCGGCCA ACAGGAATCT ATGGGGAACA GCATCAGCTG 
ATGAAGGACT TCTATCTGAA CAGTGTCCGT AATGGCGGTT GGGTGATGAG AGGAGTTCCT 
CCTCATACGG AGCATGGACG CGTCTATGCA GGTAATGTGG CGTGGATGCA TTTACTAGCA 
GCACGTGCTC TGCAGGAACA TCCCAATCGG CTGGGTGGCG AATGTTATTT CTGCTATGAT 
GACTCGCCAT ACAAGCCCTA CGATGAATTC AACATGCAGT TTCTGTCAGC CTTCAACTTT 
CGCTCGCTTC GCCTGCCAGT GTGGATGCTG TGGATCATAG CATGGATGAA CGACATGGTC 
CGCTGGGTCC TGAAGCCCAT CTACAACTAC ACACCACTGC TCAACAAATA CACACTGGCT 
GTGGCCTGCA CATCCTTCAC CGTCAGCACA GACAAGGCTT TTCGGCACTT TCAGTACCAG 
CCGCTCTACA GCTGGCAGCA GTGCCTGTCT CGTACGCAGA GCTGGGTCAA CACTTTCCCA 
TTTGAAACCA GCACTAAAGA CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009297795.1
CDS	101..1207
Translation	MSNNNKSKLTYVITGGCGFLGQHLLRVLLEKEKNVKEIRLFDKNVFPSLQSESTEDVKVVIIQGDITKYEDVRNAFLGADLVFHAASLVDVWYKIPEKVIFAVNVQGTENAIKACVEIGIQYLVYTSSMEVVGPNVKGDEFVRGNEDTPYNIFHEMPYPKSKAAAEKIVLEANGTKVEGGNILYTCCLRPTGIYGEQHQLMKDFYLNSVRNGGWVMRGVPPHTEHGRVYAGNVAWMHLLAARALQEHPNRLGGECYFCYDDSPYKPYDEFNMQFLSAFNFRSLRLPVWMLWIIAWMNDMVRWVLKPIYNYTPLLNKYTLAVACTSFTVSTDKAFRHFQYQPLYSWQQCLSRTQSWVNTFPFETSTKDK

Target ORF information:

RefSeq Version	XM_009299520.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase (hsd3b7), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299520.2

ORF Insert Sequence:

ATGTCCAACA ACAACAAGTC GAAGTTAACT TATGTCATAA CTGGAGGATG CGGTTTTCTG 
GGGCAGCACC TGTTGAGGGT TTTGCTGGAG AAGGAAAAAA ATGTCAAGGA GATCAGGCTT 
TTCGACAAAA ACGTTTTCCC AAGTCTTCAG AGTGAGAGTA CAGAGGATGT GAAAGTGGTC 
ATCATACAGG GAGATATAAC AAAATATGAG GATGTACGCA ATGCATTCCT GGGAGCAGAC 
CTGGTTTTCC ATGCCGCAAG TTTGGTTGAT GTGTGGTATA AAATTCCAGA GAAGGTCATC 
TTTGCTGTCA ATGTACAAGG GACGGAGAAT GCTATAAAAG CCTGTGTAGA AATCGGTATT 
CAGTATTTGG TCTACACAAG CTCCATGGAG GTGGTTGGTC CAAATGTAAA GGGAGATGAA 
TTTGTCAGAG GGAATGAGGA TACTCCATAT AATATATTCC ATGAGATGCC CTACCCTAAA 
TCCAAAGCTG CAGCTGAGAA AATTGTGCTG GAAGCCAATG GCACAAAGGT CGAGGGAGGG 
AACATTCTCT ACACATGCTG TCTGCGGCCA ACAGGAATCT ATGGGGAACA GCATCAGCTG 
ATGAAGGACT TCTATCTGAA CAGTGTCCGT AATGGCGGTT GGGTGATGAG AGGAGTTCCT 
CCTCATACGG AGCATGGACG CGTCTATGCA GGTAATGTGG CGTGGATGCA TTTACTAGCA 
GCACGTGCTC TGCAGGAACA TCCCAATCGG CTGGGTGGCG AATGTTATTT CTGCTATGAT 
GACTCGCCAT ACAAGCCCTA CGATGAATTC AACATGCAGT TTCTGTCAGC CTTCAACTTT 
CGCTCGCTTC GCCTGCCAGT GTGGATGCTG TGGATCATAG CATGGATGAA CGACATGGTC 
CGCTGGGTCC TGAAGCCCAT CTACAACTAC ACACCACTGC TCAACAAATA CACACTGGCT 
GTGGCCTGCA CATCCTTCAC CGTCAGCACA GACAAGGCTT TTCGGCACTT TCAGTACCAG 
CCGCTCTACA GCTGGCAGCA GTGCCTGTCT CGTACGCAGA GCTGGGTCAA CACTTTCCCA 
TTTGAAACCA GCACTAAAGA CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hsd3b7 ( XM_017353437.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017208926.1 Danio rerio hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase (hsd3b7), transcript variant X3, mRNA.

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CloneID	ODa39918
Clone ID Related Accession (Same CDS sequence)	XM_017353437.1 , XM_009299519.2 , XM_009299519.3 , XM_009299518.2 , XM_009299518.3 , XM_009299520.2 , XM_017353437.2
Accession Version	XM_017353437.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	3 beta-hydroxysteroid dehydrogenase type 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017353437.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAACA ACAACAAGTC GAAGTTAACT TATGTCATAA CTGGAGGATG CGGTTTTCTG 
GGGCAGCACC TGTTGAGGGT TTTGCTGGAG AAGGAAAAAA ATGTCAAGGA GATCAGGCTT 
TTCGACAAAA ACGTTTTCCC AAGTCTTCAG AGTGAGAGTA CAGAGGATGT GAAAGTGGTC 
ATCATACAGG GAGATATAAC AAAATATGAG GATGTACGCA ATGCATTCCT GGGAGCAGAC 
CTGGTTTTCC ATGCCGCAAG TTTGGTTGAT GTGTGGTATA AAATTCCAGA GAAGGTCATC 
TTTGCTGTCA ATGTACAAGG GACGGAGAAT GCTATAAAAG CCTGTGTAGA AATCGGTATT 
CAGTATTTGG TCTACACAAG CTCCATGGAG GTGGTTGGTC CAAATGTAAA GGGAGATGAA 
TTTGTCAGAG GGAATGAGGA TACTCCATAT AATATATTCC ATGAGATGCC CTACCCTAAA 
TCCAAAGCTG CAGCTGAGAA AATTGTGCTG GAAGCCAATG GCACAAAGGT CGAGGGAGGG 
AACATTCTCT ACACATGCTG TCTGCGGCCA ACAGGAATCT ATGGGGAACA GCATCAGCTG 
ATGAAGGACT TCTATCTGAA CAGTGTCCGT AATGGCGGTT GGGTGATGAG AGGAGTTCCT 
CCTCATACGG AGCATGGACG CGTCTATGCA GGTAATGTGG CGTGGATGCA TTTACTAGCA 
GCACGTGCTC TGCAGGAACA TCCCAATCGG CTGGGTGGCG AATGTTATTT CTGCTATGAT 
GACTCGCCAT ACAAGCCCTA CGATGAATTC AACATGCAGT TTCTGTCAGC CTTCAACTTT 
CGCTCGCTTC GCCTGCCAGT GTGGATGCTG TGGATCATAG CATGGATGAA CGACATGGTC 
CGCTGGGTCC TGAAGCCCAT CTACAACTAC ACACCACTGC TCAACAAATA CACACTGGCT 
GTGGCCTGCA CATCCTTCAC CGTCAGCACA GACAAGGCTT TTCGGCACTT TCAGTACCAG 
CCGCTCTACA GCTGGCAGCA GTGCCTGTCT CGTACGCAGA GCTGGGTCAA CACTTTCCCA 
TTTGAAACCA GCACTAAAGA CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208926.1
CDS	618..1724
Translation	MSNNNKSKLTYVITGGCGFLGQHLLRVLLEKEKNVKEIRLFDKNVFPSLQSESTEDVKVVIIQGDITKYEDVRNAFLGADLVFHAASLVDVWYKIPEKVIFAVNVQGTENAIKACVEIGIQYLVYTSSMEVVGPNVKGDEFVRGNEDTPYNIFHEMPYPKSKAAAEKIVLEANGTKVEGGNILYTCCLRPTGIYGEQHQLMKDFYLNSVRNGGWVMRGVPPHTEHGRVYAGNVAWMHLLAARALQEHPNRLGGECYFCYDDSPYKPYDEFNMQFLSAFNFRSLRLPVWMLWIIAWMNDMVRWVLKPIYNYTPLLNKYTLAVACTSFTVSTDKAFRHFQYQPLYSWQQCLSRTQSWVNTFPFETSTKDK

Target ORF information:

RefSeq Version	XM_017353437.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase (hsd3b7), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353437.2

ORF Insert Sequence:

ATGTCCAACA ACAACAAGTC GAAGTTAACT TATGTCATAA CTGGAGGATG CGGTTTTCTG 
GGGCAGCACC TGTTGAGGGT TTTGCTGGAG AAGGAAAAAA ATGTCAAGGA GATCAGGCTT 
TTCGACAAAA ACGTTTTCCC AAGTCTTCAG AGTGAGAGTA CAGAGGATGT GAAAGTGGTC 
ATCATACAGG GAGATATAAC AAAATATGAG GATGTACGCA ATGCATTCCT GGGAGCAGAC 
CTGGTTTTCC ATGCCGCAAG TTTGGTTGAT GTGTGGTATA AAATTCCAGA GAAGGTCATC 
TTTGCTGTCA ATGTACAAGG GACGGAGAAT GCTATAAAAG CCTGTGTAGA AATCGGTATT 
CAGTATTTGG TCTACACAAG CTCCATGGAG GTGGTTGGTC CAAATGTAAA GGGAGATGAA 
TTTGTCAGAG GGAATGAGGA TACTCCATAT AATATATTCC ATGAGATGCC CTACCCTAAA 
TCCAAAGCTG CAGCTGAGAA AATTGTGCTG GAAGCCAATG GCACAAAGGT CGAGGGAGGG 
AACATTCTCT ACACATGCTG TCTGCGGCCA ACAGGAATCT ATGGGGAACA GCATCAGCTG 
ATGAAGGACT TCTATCTGAA CAGTGTCCGT AATGGCGGTT GGGTGATGAG AGGAGTTCCT 
CCTCATACGG AGCATGGACG CGTCTATGCA GGTAATGTGG CGTGGATGCA TTTACTAGCA 
GCACGTGCTC TGCAGGAACA TCCCAATCGG CTGGGTGGCG AATGTTATTT CTGCTATGAT 
GACTCGCCAT ACAAGCCCTA CGATGAATTC AACATGCAGT TTCTGTCAGC CTTCAACTTT 
CGCTCGCTTC GCCTGCCAGT GTGGATGCTG TGGATCATAG CATGGATGAA CGACATGGTC 
CGCTGGGTCC TGAAGCCCAT CTACAACTAC ACACCACTGC TCAACAAATA CACACTGGCT 
GTGGCCTGCA CATCCTTCAC CGTCAGCACA GACAAGGCTT TTCGGCACTT TCAGTACCAG 
CCGCTCTACA GCTGGCAGCA GTGCCTGTCT CGTACGCAGA GCTGGGTCAA CACTTTCCCA 
TTTGAAACCA GCACTAAAGA CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Protein Arginine Methyltransferase 6 (Prmt6) Is Essential for Early Zebrafish Development through the Direct Suppression of gadd45?a Stress Sensor Gene.
The Journal of biological chemistry291(1)402-12(2016 Jan)
Zhao XX,Zhang YB,Ni PL,Wu ZL,Yan YC,Li YP

Identification of differentially expressed genes in the zebrafish hypothalamic-pituitary axis.
Gene expression patterns : GEP9(4)200-8(2009 Apr)
Toro S,Wegner J,Muller M,Westerfield M,Varga ZM

Hepatic gene expression profiling using GeneChips in zebrafish exposed to 17alpha-methyldihydrotestosterone.
Aquatic toxicology (Amsterdam, Netherlands)87(2)69-80(2008 Apr)
Hoffmann JL,Thomason RG,Lee DM,Brill JL,Price BB,Carr GJ,Versteeg DJ

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hsd3b7 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

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