Smpdl3b cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Smpdl3b
Entrez Gene ID	100340
Full Name	sphingomyelin phosphodiesterase, acid-like 3B
Synonyms	1110054A24Rik,AU045240,Asml3b
General protein information	Preferred Names sphingomyelin phosphodiesterase, acid-like 3B Names acid sphingomyelinase-like phosphodiesterase 3b ASM-like phosphodiesterase 3b
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 D2.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_133888.2	NP_598649.1	acid sphingomyelinase-like phosphodiesterase 3b precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SMPDL3B	NP_001093849.1
Caenorhabditis elegans (roundworm)	ZK856.5	NP_505620.4
Xenopus tropicalis (tropical clawed frog)	smpdl3b	XP_002939398.1
Homo sapiens (human)	SMPDL3B	NP_055289.2
Canis lupus familiaris (dog)	SMPDL3B	XP_005617767.1
Drosophila melanogaster (fruit fly)	CG32052	NP_729555.4
Mus musculus (house mouse)	Smpdl3b	NP_598649.1
Rattus norvegicus (Norway rat)	Smpdl3b	NP_001020908.1
Danio rerio (zebrafish)	LOC564392	XP_692822.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005806	XP_315822.5
Pan troglodytes (chimpanzee)	SMPDL3B	XP_001151336.1
Gallus gallus (chicken)	SMPDL3B	NP_001239209.1

Gene Ontology
Bibliography

Related articles in PubMed

Sphingomyelinase-like phosphodiesterase 3b mediates radiation-induced damage of renal podocytes.
Ahmad A, Mitrofanova A, Bielawski J, Yang Y, Marples B, Fornoni A, Zeidan YH
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(2)771-780(2017 02)

Crystal Structure of the Acid Sphingomyelinase-like Phosphodiesterase SMPDL3B Provides Insights into Determinants of Substrate Specificity.
Gorelik A, Heinz LX, Illes K, Superti-Furga G, Nagar B
The Journal of biological chemistry291(46)24054-24064(2016 Nov)

The Lipid-Modifying Enzyme SMPDL3B Negatively Regulates Innate Immunity.
Heinz LX, Baumann CL, K?berlin MS, Snijder B, Gawish R, Shui G, Sharif O, Aspalter IM, M?ller AC, Kandasamy RK, Breitwieser FP, Pichlmair A, Bruckner M, Rebsamen M, Bl?ml S, Karonitsch T, Fauster A, Colinge J, Bennett KL, Knapp S, Wenk MR, Superti-Furga G
Cell reports11(12)1919-28(2015 Jun)

A Grhl2-dependent gene network controls trophoblast branching morphogenesis.
Walentin K, Hinze C, Werth M, Haase N, Varma S, Morell R, Aue A, P?tschke E, Warburton D, Qiu A, Barasch J, Purf?rst B, Dieterich C, Popova E, Bader M, Dechend R, Staff AC, Yurtdas ZY, Kilic E, Schmidt-Ott KM
Development (Cambridge, England)142(6)1125-36(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results demonstrate that radiation injury induces early cytoskeletal remodeling, down-regulation of SMPDL3b, and elevation of cellular ceramide levels. Overexpression of SMPDL3b and pretreatment with rituximab confer a radioprotective effect in cultured podocytes.
Title: Sphingomyelinase-like phosphodiesterase 3b mediates radiation-induced damage of renal podocytes.

Although a potential substrate for SMPDL3B is sphingomyelin, we identify other possible substrates such as CDP-choline, ATP, and ADP.
Title: Crystal Structure of the Acid Sphingomyelinase-like Phosphodiesterase SMPDL3B Provides Insights into Determinants of Substrate Specificity.

our results identify the membrane-modulating enzyme SMPDL3B as a negative regulator of TLR signaling that functions at the interface of membrane biology and innate immunity.
Title: The Lipid-Modifying Enzyme SMPDL3B Negatively Regulates Innate Immunity.

SMPDL3b may be an important modulator of podocyte function by shifting suPAR-mediated podocyte injury from a migratory phenotype to an apoptotic phenotype and that it represents a novel therapeutic glomerular disease target
Title: Sphingomyelinase-like phosphodiesterase 3b expression levels determine podocyte injury phenotypes in glomerular disease.

The following Smpdl3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Smpdl3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu10310	NM_133888.2 Latest version!	Mus musculus sphingomyelin phosphodiesterase, acid-like 3B (Smpdl3b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$272.30 $389.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Smpdl3b ( NM_133888.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_598649.1 Mus musculus sphingomyelin phosphodiesterase, acid-like 3B (Smpdl3b), mRNA.

Price & Availability↑

CloneID	OMu10310
Clone ID Related Accession (Same CDS sequence)	NM_133888.2
Accession Version	NM_133888.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1371bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-15
Organism	Mus musculus(house mouse)
Product	acid sphingomyelinase-like phosphodiesterase 3b precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL627130.13. On Nov 17, 2006 this sequence version replaced NM_133888.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC009087.1, AK154599.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN01164133 [ECO:0000348] ##Evidence-Data-END##

ATGACGCTGC TCGGGTGGCT GATATTCCTG GCCCCCTGGG GAGTCGCAGG GGCTCAACTA 
GGGAGGTTCT GGCACATCTC CGACCTGCAT CTGGACCCCA ACTACACCGT ATCCAAAGAC 
CCCCTCCAGG TGTGCCCGTC GGCCGGCTCC CAGCCTGTGC TAAATGCTGG CCCCTGGGGG 
GACTACCTCT GCGATTCTCC TTGGGCCCTT ATCAACTCGT CCTTGTATGC CATGAAGGAG 
ATTGAACCAA AGCCTGACTT CATTCTCTGG ACAGGGGACG ACACACCGCA CGTCCCCAAT 
GAGAGTCTAG GAGAGGCAGC TGTGCTGGCA ATTGTGGAAC GCTTGACCAA CCTCATCAAG 
GAAGTCTTTC CAGACACTAA AGTCTATGCT GCTCTGGGAA ATCATGACTT CCACCCTAAG 
AACCAGTTCC CAGCACAGAG CAACCGCATC TATAACCAGG TGGCAGAGCT GTGGAGACCC 
TGGCTTAGTA ACGAATCCTA CGCTCTCTTC AAAAGAGGTG CCTTCTATTC TGAGAAGTTG 
CCGGGTCCCA GCAGGGCGGG GCGAGTTGTG GTCCTCAACA CCAATCTGTA CTACAGCAAC 
AACGAGCAGA CAGCTGGCAT GGCTGACCCC GGCGAGCAGT TCCGGTGGCT GGGAGATGTC 
CTGAGCAATG CATCTCGGGA TGGGGAGATG GTGTATGTTA TTGGCCACGT GCCCCCGGGG 
TTCTTTGAGA AGACACAGAA CAAGGCCTGG TTCCGAGAGA GCTTCAATGA GGAGTATCTG 
AAGGTGATCC AGAAGCACCA TCGGGTCATA GCAGGGCAGT TCTTTGGACA CCACCATACC 
GACAGCTTCC GAATGTTCTA TGACAACACA GGTGCCCCCA TAAACGTCAT GTTTCTCACA 
CCCGGGGTCA CACCGTGGAA GACCACATTA CCTGGAGTGG TCGATGGGGC CAACAATCCA 
GGGATACGCA TTTTCGAGTA TGATCGAGCC ACACTCAACT TGAAGGACTT GGTGACTTAC 
TTCTTGAACC TGAGGCAGGC GAATGTACAA GAGACCCCAC GGTGGGAGCA GGAGTACCGC 
CTGACGGAGG CCTACCAGGT GCCGGATGCC AGCGTCAGCT CCATGCACAC GGCGCTGACC 
CGCATTGCCA GTGAGCCTCA CATCCTGCAA CGTTATTACG TCTATAACTC GGTCAGCTAC 
AACCATTTGA CCTGTGAGGA CAGCTGCCGC ATCGAGCACG TTTGTGCTAT ACAACACGTG 
GCTTTCAACA CTTATGCTAC CTGCTTGCAT GGTCTTGGTG CCAAGCTGGT GCCTGGTTTC 
CTGCTCATAC TGACTCTGCT GCCAAGCCTG CACGTACTGG AGGTGTTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598649.1
CDS	127..1497
Translation	MTLLGWLIFLAPWGVAGAQLGRFWHISDLHLDPNYTVSKDPLQVCPSAGSQPVLNAGPWGDYLCDSPWALINSSLYAMKEIEPKPDFILWTGDDTPHVPNESLGEAAVLAIVERLTNLIKEVFPDTKVYAALGNHDFHPKNQFPAQSNRIYNQVAELWRPWLSNESYALFKRGAFYSEKLPGPSRAGRVVVLNTNLYYSNNEQTAGMADPGEQFRWLGDVLSNASRDGEMVYVIGHVPPGFFEKTQNKAWFRESFNEEYLKVIQKHHRVIAGQFFGHHHTDSFRMFYDNTGAPINVMFLTPGVTPWKTTLPGVVDGANNPGIRIFEYDRATLNLKDLVTYFLNLRQANVQETPRWEQEYRLTEAYQVPDASVSSMHTALTRIASEPHILQRYYVYNSVSYNHLTCEDSCRIEHVCAIQHVAFNTYATCLHGLGAKLVPGFLLILTLLPSLHVLEVL

Target ORF information:

RefSeq Version	NM_133888.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus sphingomyelin phosphodiesterase, acid-like 3B (Smpdl3b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133888.2

ORF Insert Sequence:

ATGACGCTGC TCGGGTGGCT GATATTCCTG GCCCCCTGGG GAGTCGCAGG GGCTCAACTA 
GGGAGGTTCT GGCACATCTC CGACCTGCAT CTGGACCCCA ACTACACCGT ATCCAAAGAC 
CCCCTCCAGG TGTGCCCGTC GGCCGGCTCC CAGCCTGTGC TAAATGCTGG CCCCTGGGGG 
GACTACCTCT GCGATTCTCC TTGGGCCCTT ATCAACTCGT CCTTGTATGC CATGAAGGAG 
ATTGAACCAA AGCCTGACTT CATTCTCTGG ACAGGGGACG ACACACCGCA CGTCCCCAAT 
GAGAGTCTAG GAGAGGCAGC TGTGCTGGCA ATTGTGGAAC GCTTGACCAA CCTCATCAAG 
GAAGTCTTTC CAGACACTAA AGTCTATGCT GCTCTGGGAA ATCATGACTT CCACCCTAAG 
AACCAGTTCC CAGCACAGAG CAACCGCATC TATAACCAGG TGGCAGAGCT GTGGAGACCC 
TGGCTTAGTA ACGAATCCTA CGCTCTCTTC AAAAGAGGTG CCTTCTATTC TGAGAAGTTG 
CCGGGTCCCA GCAGGGCGGG GCGAGTTGTG GTCCTCAACA CCAATCTGTA CTACAGCAAC 
AACGAGCAGA CAGCTGGCAT GGCTGACCCC GGCGAGCAGT TCCGGTGGCT GGGAGATGTC 
CTGAGCAATG CATCTCGGGA TGGGGAGATG GTGTATGTTA TTGGCCACGT GCCCCCGGGG 
TTCTTTGAGA AGACACAGAA CAAGGCCTGG TTCCGAGAGA GCTTCAATGA GGAGTATCTG 
AAGGTGATCC AGAAGCACCA TCGGGTCATA GCAGGGCAGT TCTTTGGACA CCACCATACC 
GACAGCTTCC GAATGTTCTA TGACAACACA GGTGCCCCCA TAAACGTCAT GTTTCTCACA 
CCCGGGGTCA CACCGTGGAA GACCACATTA CCTGGAGTGG TCGATGGGGC CAACAATCCA 
GGGATACGCA TTTTCGAGTA TGATCGAGCC ACACTCAACT TGAAGGACTT GGTGACTTAC 
TTCTTGAACC TGAGGCAGGC GAATGTACAA GAGACCCCAC GGTGGGAGCA GGAGTACCGC 
CTGACGGAGG CCTACCAGGT GCCGGATGCC AGCGTCAGCT CCATGCACAC GGCGCTGACC 
CGCATTGCCA GTGAGCCTCA CATCCTGCAA CGTTATTACG TCTATAACTC GGTCAGCTAC 
AACCATTTGA CCTGTGAGGA CAGCTGCCGC ATCGAGCACG TTTGTGCTAT ACAACACGTG 
GCTTTCAACA CTTATGCTAC CTGCTTGCAT GGTCTTGGTG CCAAGCTGGT GCCTGGTTTC 
CTGCTCATAC TGACTCTGCT GCCAAGCCTG CACGTACTGG AGGTGTTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sphingomyelinase-like phosphodiesterase 3b mediates radiation-induced damage of renal podocytes.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology31(2)771-780(2017 02)
Ahmad A,Mitrofanova A,Bielawski J,Yang Y,Marples B,Fornoni A,Zeidan YH

Crystal Structure of the Acid Sphingomyelinase-like Phosphodiesterase SMPDL3B Provides Insights into Determinants of Substrate Specificity.
The Journal of biological chemistry291(46)24054-24064(2016 Nov)
Gorelik A,Heinz LX,Illes K,Superti-Furga G,Nagar B

The Lipid-Modifying Enzyme SMPDL3B Negatively Regulates Innate Immunity.
Cell reports11(12)1919-28(2015 Jun)
Heinz LX,Baumann CL,K?berlin MS,Snijder B,Gawish R,Shui G,Sharif O,Aspalter IM,M?ller AC,Kandasamy RK,Breitwieser FP,Pichlmair A,Bruckner M,Rebsamen M,Bl?ml S,Karonitsch T,Fauster A,Colinge J,Bennett KL,Knapp S,Wenk MR,Superti-Furga G

A Grhl2-dependent gene network controls trophoblast branching morphogenesis.
Development (Cambridge, England)142(6)1125-36(2015 Mar)
Walentin K,Hinze C,Werth M,Haase N,Varma S,Morell R,Aue A,P?tschke E,Warburton D,Qiu A,Barasch J,Purf?rst B,Dieterich C,Popova E,Bader M,Dechend R,Staff AC,Yurtdas ZY,Kilic E,Schmidt-Ott KM

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Smpdl3b cDNA ORF clone, Mus musculus(house mouse)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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