BUB1B cDNA ORF clone, Mesitornis unicolor(brown roatelo)

Gene
Clones
gRNAs

Summary

Gene Symbol	BUB1B
Entrez Gene ID	104537097
Full Name	BUB1 mitotic checkpoint serine/threonine kinase B
Gene Type	protein-coding
Organism	Mesitornis unicolor(brown roatelo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010179883.1	XP_010178185.1	mitotic checkpoint serine/threonine-protein kinase BUB1 beta

Pathways from BioSystems
Gene Ontology

The following BUB1B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BUB1B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMe78537	XM_010179883.1 Latest version!	Mesitornis unicolor BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BUB1B ( XM_010179883.1 ) cDNA ORF clone, Mesitornis unicolor(brown roatelo) -> XP_010178185.1 Mesitornis unicolor BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), mRNA.

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CloneID	OMe78537
Clone ID Related Accession (Same CDS sequence)	XM_010179883.1
Accession Version	XM_010179883.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-11-05
Organism	Mesitornis unicolor(brown roatelo)
Product	mitotic checkpoint serine/threonine-protein kinase BUB1 beta
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_010171078.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mesitornis unicolor Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACAGG ACTTCAGTGA TGAGTGGGAG CTGAGCAAAG AGAATGTGCA GCCCCTGCGG 
CAAGGACGTG TCATGTCCGC CTTGCAGGAG GCACTGGCTC ATCAGGAGAC CTCCACCCAC 
ACTGCTGTTC ACCTCAAAAA ACAGGAGTTT GAATCAGAAA TCCGATTTTA CTCTGGAGAT 
GATCCACTGG ATGTCTGGGA CCGGTACATC AAGTGGACAG AGCAAACCTT CCCCCAAGGT 
GGAAAGGAGA GTAATCTCGC AGCAATATTG GAAAGGGCAG TGAAAGCACT CAATGAAGAG 
CAGCGATACT ACAAAGATCC CCGCTATCTT AATCTCTGGC TCAAGTTTGG AGATTGCTGT 
AATGAGCCCT TGGATCTGTA CAGTTACCTA CACAGCCAGG AAATTGGCAC AACACTGGCA 
CTACTCTACA TCACTTGGGC AGAAGTACTT GAGGCGAGAG GAAGTTTTAA GAAAGCAGAT 
CTGATATTCC AGGAAGGTCT TCAGCGCAAA GCTGAGCCTT TGGACAAACT CCAGTTCCAT 
CACAGGCAGT TTCAGATCCG TGTTTCTCGA CAGACGCTAT TGGGGCTAGA GGAAACTCCT 
AATAGAGACA CCGGTCTTCT GGGAGTTGTA GAACCTCAGA GAACCTCATT GGCAGATCTA 
AAAGGCAAGG GGAATAAGAA AGTGAGAGCC CCAATTAGTC GCGTAGGAGA TGCACTTAAA 
GCCACAAGCC AAACCAGAAG CTTCCAGGCT GCAACTTCTC AGCAGCTTTT AAACAATCCT 
GATTTTGCTG TATTTGATGA AAATTCAGCC TCTGGATCTG AGATGCCCAT GCTTACACCA 
CAGCCATGGA CAGTACCTCC AGCTCCACGG GCCAAGGAGA ATGAAATGAG TGCAGGACCT 
TGGAACTCCG GCAGGCGTCC TCGCAACAGT GCAAATTCTG GTATAGAAGT GCCCTACCCA 
CTGCCCAGTT TCACACCCTA TGTGGAAGAG TCAGCTCAGC AACAAGTCAT GACTCCCTGC 
AAAATTGAGC CCAGCATAAA CTGTGTTCTA AGTGCTCGCA AACCTGAGAA AGAGGAGGAT 
CCACTACAGC GAGTGCAAAA TCATCAGAAG GATACTCAAG ACAAGACAGA GGTGATGTAC 
TGTAAAGATA AGGTTTATGC TGGAGTCGAA GAATTTTCTT TGGAAGAGAT TCGAGCTGAA 
ATCTACAGAA AGAAAGTATT AAAGAAAACT GAAGAGGAGA TACAGGACAT AGCACAGAAG 
AAGGAAGAAA TACAAAAGAA AATTGAGGAC CTGGAGAAGA AATTAAAGAA GAAAGAAGAT 
GACAAGCAGC AACAACCACA AGAACAGCCA ACAGAGATAA CAGAAGCTTC ACCACCTTTG 
GGACTGCAAG GATTTACTTT TCTGAGTGCA ACAGAAGTTG AGGAGAAACA GCCTCAGTTG 
CAAAGTGAAT CCTCAGTCTC TGAAGATACT AAACTGCATA AACCATCTTG TTCTGAGGAT 
ACGCAAAGAG GAAATGTGTT TTTAGACTCT GAGAATGAAC AGGAGGAACA GAAGGATGAG 
GCCAGCCTTG GAAAAGAAGG TACAATTCTG AAGTGTCATC TTCCCCCAGC GGATCCTGCT 
ACGTTTTTCA CCATATTTGA TGAATCCTCT ACTTTGACAG ATCAGACTAT AAGTAGTTCT 
GCAGATCCTA CACAGAAAAG TGCCCAGCGC CCTCTTGCCA TTCGTAAAGC TTCAGATTCT 
CTAACTGCAG AGGAAAACGT ATCACCAGAA GCCTGTGATG AGCTGAATGG AATTGAACCT 
TTGACTGAAG ATGCAATTGT GACTAGCTCC TACAAGAACA AAACCCTTTG TGCCAACCCA 
GAAGACACGT GTGACTTTGT TAGGGCTGCC CACTTGGCCT CAACGCCCTT TCATGGGGTG 
GTAGCTCAGA GAGTCCCAGC TCCTGCTTTT TCAGAGAGTG TCCTGAAGGA AGACTCCCTA 
GAATCTAAGA CTGCATCTCT GAATCAGGAA ACACCGGTCT GTGAGGGAGC ATTCAGTGAA 
GCGCTTTGTA TAAAGAAACT CAGCCCGATC ATGGAAGCAA GCCTGGAAGA TACTCGCTCG 
TCAGGATCTT CTGTCTCCTC AGGATCTTCT CTTTCCTCTG TTACACAAAT ATCTACTATA 
AAATACTTGC ATATCCCGGA GAAACTGGAA TTTACTCATA GCTTGCCTGC AGAAATGGTT 
ACTGATTCTG GAGGTGATGA CGTAACTCAG GTCTTGTGGT GCACTGAACG GCGTAAAGAG 
CTTTTAGATC CTATGCCAGA ATCATTGACT GCTTCTCCAG ACTTTCATTT GGAGGCTGGC 
GCTCTGCCTG TCATGCAGTT TGAGAAAAAC GTTGAACTAG GAACTGACAC TTACTGTATC 
AAATGGGAAT ATTGGACCAA TGAAGAACAC AAGATGTTTT TTGCCATTCT AGAGAACTCT 
CCCCGGTTGG ATACAAAGGG ATTTGCGATA AAGGTGCATT CTCAGCCTGT GCCTTGGGAT 
TTTTATATCA CACTTCAATT GCAGGAGCGT TTGAATTCTG ACTTTGAGCA AAGCTTCAGT 
GAGAATTTCA GCTGCTACTT GTATCAAGAC GGCTGTGCTA TTTTGCACAG GAATATAAAT 
TACTTTACGC TTGGGGATGT TATTCGTGGC TGTAAGTCCA TCACAGATGA TGTTATCCTA 
CTGGTCGTTT ATAATCTTCT GGGCGTGGTA GAGAAGCTCC ATAGAGCAGA GATTGTCCAT 
GGAGACCTGA GTCCAGAGGT GTTCTTTCTG GGAGACAGGA TCTATGATCC TTTTGGTAAT 
GATGAGATGT CAAGAGCTCT GAAGATCGTG GATTTCTCTC ACAGTCTAGA TTTGAGATTA 
CAGTCTCGAG TAAGTTTGCC CAACAGCTTT CCAATATCTC AGACCCCTTA TGGACAACAG 
CTTCTGACAG AAAGTTCTCT TCCTTATCAA GTAGACTTGG TTGGCATTGC AGATATAGTC 
CATTTGATGC TCTTTGGGGA TCATCTCCAG GTCTATCAAG AGAATTCCAT CTGGAAAATC 
AGCCAAAATG TATCCAAGAC TGTTGACAGT GATTTTTGGA GTAAATTCTT TGGGAGAATT 
CTGAATGCAG ATGGTAAGTC CACAGTGCCT CTGCTGAGGG AGTTGAGAGA GGAAGTGCGT 
GCCATGTTTG ACAGCTGTTT CCAAGAACGC CTTTGTGACT CCTTAACTGC ACTGGGAGAA 
ACTCTCTTGG AGAACACCCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010178185.1
CDS	1..3264
Translation	MSQDFSDEWELSKENVQPLRQGRVMSALQEALAHQETSTHTAVHLKKQEFESEIRFYSGDDPLDVWDRYIKWTEQTFPQGGKESNLAAILERAVKALNEEQRYYKDPRYLNLWLKFGDCCNEPLDLYSYLHSQEIGTTLALLYITWAEVLEARGSFKKADLIFQEGLQRKAEPLDKLQFHHRQFQIRVSRQTLLGLEETPNRDTGLLGVVEPQRTSLADLKGKGNKKVRAPISRVGDALKATSQTRSFQAATSQQLLNNPDFAVFDENSASGSEMPMLTPQPWTVPPAPRAKENEMSAGPWNSGRRPRNSANSGIEVPYPLPSFTPYVEESAQQQVMTPCKIEPSINCVLSARKPEKEEDPLQRVQNHQKDTQDKTEVMYCKDKVYAGVEEFSLEEIRAEIYRKKVLKKTEEEIQDIAQKKEEIQKKIEDLEKKLKKKEDDKQQQPQEQPTEITEASPPLGLQGFTFLSATEVEEKQPQLQSESSVSEDTKLHKPSCSEDTQRGNVFLDSENEQEEQKDEASLGKEGTILKCHLPPADPATFFTIFDESSTLTDQTISSSADPTQKSAQRPLAIRKASDSLTAEENVSPEACDELNGIEPLTEDAIVTSSYKNKTLCANPEDTCDFVRAAHLASTPFHGVVAQRVPAPAFSESVLKEDSLESKTASLNQETPVCEGAFSEALCIKKLSPIMEASLEDTRSSGSSVSSGSSLSSVTQISTIKYLHIPEKLEFTHSLPAEMVTDSGGDDVTQVLWCTERRKELLDPMPESLTASPDFHLEAGALPVMQFEKNVELGTDTYCIKWEYWTNEEHKMFFAILENSPRLDTKGFAIKVHSQPVPWDFYITLQLQERLNSDFEQSFSENFSCYLYQDGCAILHRNINYFTLGDVIRGCKSITDDVILLVVYNLLGVVEKLHRAEIVHGDLSPEVFFLGDRIYDPFGNDEMSRALKIVDFSHSLDLRLQSRVSLPNSFPISQTPYGQQLLTESSLPYQVDLVGIADIVHLMLFGDHLQVYQENSIWKISQNVSKTVDSDFWSKFFGRILNADGKSTVPLLRELREEVRAMFDSCFQERLCDSLTALGETLLENTL

Target ORF information:

RefSeq Version	XM_010179883.1
Organism	Mesitornis unicolor(brown roatelo)
Definition	Mesitornis unicolor BUB1 mitotic checkpoint serine/threonine kinase B (BUB1B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010179883.1

ORF Insert Sequence:

ATGTCACAGG ACTTCAGTGA TGAGTGGGAG CTGAGCAAAG AGAATGTGCA GCCCCTGCGG 
CAAGGACGTG TCATGTCCGC CTTGCAGGAG GCACTGGCTC ATCAGGAGAC CTCCACCCAC 
ACTGCTGTTC ACCTCAAAAA ACAGGAGTTT GAATCAGAAA TCCGATTTTA CTCTGGAGAT 
GATCCACTGG ATGTCTGGGA CCGGTACATC AAGTGGACAG AGCAAACCTT CCCCCAAGGT 
GGAAAGGAGA GTAATCTCGC AGCAATATTG GAAAGGGCAG TGAAAGCACT CAATGAAGAG 
CAGCGATACT ACAAAGATCC CCGCTATCTT AATCTCTGGC TCAAGTTTGG AGATTGCTGT 
AATGAGCCCT TGGATCTGTA CAGTTACCTA CACAGCCAGG AAATTGGCAC AACACTGGCA 
CTACTCTACA TCACTTGGGC AGAAGTACTT GAGGCGAGAG GAAGTTTTAA GAAAGCAGAT 
CTGATATTCC AGGAAGGTCT TCAGCGCAAA GCTGAGCCTT TGGACAAACT CCAGTTCCAT 
CACAGGCAGT TTCAGATCCG TGTTTCTCGA CAGACGCTAT TGGGGCTAGA GGAAACTCCT 
AATAGAGACA CCGGTCTTCT GGGAGTTGTA GAACCTCAGA GAACCTCATT GGCAGATCTA 
AAAGGCAAGG GGAATAAGAA AGTGAGAGCC CCAATTAGTC GCGTAGGAGA TGCACTTAAA 
GCCACAAGCC AAACCAGAAG CTTCCAGGCT GCAACTTCTC AGCAGCTTTT AAACAATCCT 
GATTTTGCTG TATTTGATGA AAATTCAGCC TCTGGATCTG AGATGCCCAT GCTTACACCA 
CAGCCATGGA CAGTACCTCC AGCTCCACGG GCCAAGGAGA ATGAAATGAG TGCAGGACCT 
TGGAACTCCG GCAGGCGTCC TCGCAACAGT GCAAATTCTG GTATAGAAGT GCCCTACCCA 
CTGCCCAGTT TCACACCCTA TGTGGAAGAG TCAGCTCAGC AACAAGTCAT GACTCCCTGC 
AAAATTGAGC CCAGCATAAA CTGTGTTCTA AGTGCTCGCA AACCTGAGAA AGAGGAGGAT 
CCACTACAGC GAGTGCAAAA TCATCAGAAG GATACTCAAG ACAAGACAGA GGTGATGTAC 
TGTAAAGATA AGGTTTATGC TGGAGTCGAA GAATTTTCTT TGGAAGAGAT TCGAGCTGAA 
ATCTACAGAA AGAAAGTATT AAAGAAAACT GAAGAGGAGA TACAGGACAT AGCACAGAAG 
AAGGAAGAAA TACAAAAGAA AATTGAGGAC CTGGAGAAGA AATTAAAGAA GAAAGAAGAT 
GACAAGCAGC AACAACCACA AGAACAGCCA ACAGAGATAA CAGAAGCTTC ACCACCTTTG 
GGACTGCAAG GATTTACTTT TCTGAGTGCA ACAGAAGTTG AGGAGAAACA GCCTCAGTTG 
CAAAGTGAAT CCTCAGTCTC TGAAGATACT AAACTGCATA AACCATCTTG TTCTGAGGAT 
ACGCAAAGAG GAAATGTGTT TTTAGACTCT GAGAATGAAC AGGAGGAACA GAAGGATGAG 
GCCAGCCTTG GAAAAGAAGG TACAATTCTG AAGTGTCATC TTCCCCCAGC GGATCCTGCT 
ACGTTTTTCA CCATATTTGA TGAATCCTCT ACTTTGACAG ATCAGACTAT AAGTAGTTCT 
GCAGATCCTA CACAGAAAAG TGCCCAGCGC CCTCTTGCCA TTCGTAAAGC TTCAGATTCT 
CTAACTGCAG AGGAAAACGT ATCACCAGAA GCCTGTGATG AGCTGAATGG AATTGAACCT 
TTGACTGAAG ATGCAATTGT GACTAGCTCC TACAAGAACA AAACCCTTTG TGCCAACCCA 
GAAGACACGT GTGACTTTGT TAGGGCTGCC CACTTGGCCT CAACGCCCTT TCATGGGGTG 
GTAGCTCAGA GAGTCCCAGC TCCTGCTTTT TCAGAGAGTG TCCTGAAGGA AGACTCCCTA 
GAATCTAAGA CTGCATCTCT GAATCAGGAA ACACCGGTCT GTGAGGGAGC ATTCAGTGAA 
GCGCTTTGTA TAAAGAAACT CAGCCCGATC ATGGAAGCAA GCCTGGAAGA TACTCGCTCG 
TCAGGATCTT CTGTCTCCTC AGGATCTTCT CTTTCCTCTG TTACACAAAT ATCTACTATA 
AAATACTTGC ATATCCCGGA GAAACTGGAA TTTACTCATA GCTTGCCTGC AGAAATGGTT 
ACTGATTCTG GAGGTGATGA CGTAACTCAG GTCTTGTGGT GCACTGAACG GCGTAAAGAG 
CTTTTAGATC CTATGCCAGA ATCATTGACT GCTTCTCCAG ACTTTCATTT GGAGGCTGGC 
GCTCTGCCTG TCATGCAGTT TGAGAAAAAC GTTGAACTAG GAACTGACAC TTACTGTATC 
AAATGGGAAT ATTGGACCAA TGAAGAACAC AAGATGTTTT TTGCCATTCT AGAGAACTCT 
CCCCGGTTGG ATACAAAGGG ATTTGCGATA AAGGTGCATT CTCAGCCTGT GCCTTGGGAT 
TTTTATATCA CACTTCAATT GCAGGAGCGT TTGAATTCTG ACTTTGAGCA AAGCTTCAGT 
GAGAATTTCA GCTGCTACTT GTATCAAGAC GGCTGTGCTA TTTTGCACAG GAATATAAAT 
TACTTTACGC TTGGGGATGT TATTCGTGGC TGTAAGTCCA TCACAGATGA TGTTATCCTA 
CTGGTCGTTT ATAATCTTCT GGGCGTGGTA GAGAAGCTCC ATAGAGCAGA GATTGTCCAT 
GGAGACCTGA GTCCAGAGGT GTTCTTTCTG GGAGACAGGA TCTATGATCC TTTTGGTAAT 
GATGAGATGT CAAGAGCTCT GAAGATCGTG GATTTCTCTC ACAGTCTAGA TTTGAGATTA 
CAGTCTCGAG TAAGTTTGCC CAACAGCTTT CCAATATCTC AGACCCCTTA TGGACAACAG 
CTTCTGACAG AAAGTTCTCT TCCTTATCAA GTAGACTTGG TTGGCATTGC AGATATAGTC 
CATTTGATGC TCTTTGGGGA TCATCTCCAG GTCTATCAAG AGAATTCCAT CTGGAAAATC 
AGCCAAAATG TATCCAAGAC TGTTGACAGT GATTTTTGGA GTAAATTCTT TGGGAGAATT 
CTGAATGCAG ATGGTAAGTC CACAGTGCCT CTGCTGAGGG AGTTGAGAGA GGAAGTGCGT 
GCCATGTTTG ACAGCTGTTT CCAAGAACGC CTTTGTGACT CCTTAACTGC ACTGGGAGAA 
ACTCTCTTGG AGAACACCCT GTGA