LOC25495342 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC25495342
Entrez Gene ID	25495342
Full Name	LRR receptor-like kinase family protein
Gene Type	protein-coding
Organism	Medicago truncatula(barrel medic)

mRNA and Protein(s)

mRNA	Protein	Name
XM_013595557.1	XP_013451011.1	LRR receptor-like kinase family protein
XM_013595557.2	XP_013451011.1	LRR receptor-like serine/threonine-protein kinase RPK2

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
Tang H, Krishnakumar V, Bidwell S, Rosen B, Chan A, Zhou S, Gentzbittel L, Childs KL, Yandell M, Gundlach H, Mayer KF, Schwartz DC, Town CD
BMC genomics15312(2014 Apr)

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Young ND, Debell? F, Oldroyd GE, Geurts R, Cannon SB, Udvardi MK, Benedito VA, Mayer KF, Gouzy J, Schoof H, Van de Peer Y, Proost S, Cook DR, Meyers BC, Spannagl M, Cheung F, De Mita S, Krishnakumar V, Gundlach H, Zhou S, Mudge J, Bharti AK, Murray JD, Naoumkina MA, Rosen B, Silverstein KA, Tang H, Rombauts S, Zhao PX, Zhou P, Barbe V, Bardou P, Bechner M, Bellec A, Berger A, Berg?s H, Bidwell S, Bisseling T, Choisne N, Couloux A, Denny R, Deshpande S, Dai X, Doyle JJ, Dudez AM, Farmer AD, Fouteau S, Franken C, Gibelin C, Gish J, Goldstein S, Gonz?lez AJ, Green PJ, Hallab A, Hartog M, Hua A, Humphray SJ, Jeong DH, Jing Y, J?cker A, Kenton SM, Kim DJ, Klee K, Lai H, Lang C, Lin S, Macmil SL, Magdelenat G, Matthews L, McCorrison J, Monaghan EL, Mun JH, Najar FZ, Nicholson C, Noirot C, O'Bleness M, Paule CR, Poulain J, Prion F, Qin B, Qu C, Retzel EF, Riddle C, Sallet E, Samain S, Samson N, Sanders I, Saurat O, Scarpelli C, Schiex T, Segurens B, Severin AJ, Sherrier DJ, Shi R, Sims S, Singer SR, Sinharoy S, Sterck L, Viollet A, Wang BB, Wang K, Wang M, Wang X, Warfsmann J, Weissenbach J, White DD, White JD, Wiley GB, Wincker P, Xing Y, Yang L, Yao Z, Ying F, Zhai J, Zhou L, Zuber A, D?nari? J, Dixon RA, May GD, Schwartz DC, Rogers J, Qu?tier F, Town CD, Roe BA
Nature480(7378)520-4(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC25495342 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC25495342 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMe175260	XM_013595557.2 Latest version!	Medicago truncatula LRR receptor-like serine/threonine-protein kinase RPK2 (LOC25495342), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
OMe175260	XM_013595557.1	Medicago truncatula LRR receptor-like kinase family protein mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC25495342 ( XM_013595557.1 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013451011.1 Medicago truncatula LRR receptor-like kinase family protein mRNA.

Price & Availability↑

CloneID	OMe175260
Clone ID Related Accession (Same CDS sequence)	XM_013595557.1 , XM_013595557.2
Accession Version	XM_013595557.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-08-24
Organism	Medicago truncatula(barrel medic)
Product	LRR receptor-like kinase family protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_016412). ##Genome-Annotation-Data-START## Annotation Provider :: JCVI Annotation Version :: Mt4.0v2 Annotation Pipeline :: JCVI Eukaryotic Genome Annotation Pipeline Annotation Method :: EVidenceModeler (EVM), PASA Features Annotated :: Gene; mRNA; CDS; tRNA URL :: http://www.jcvi.org/medicago ##Genome-Annotation-Data-END##

ATGTTTTCAT CTTCAATTTT CATCAAATGG AGTTTCTTTC TTCTCTTCTT ATCTTCTTCA 
AACGACGCCG TTTCGTCATT TTCCGATAAA TCAACTCTCC TCCGCTTCAA AGCCTCTCTT 
TCCGACCCAT CCGCCGTTCT CTCCACCTGG AGCTCTACTG CCAACCACTG TTCCTTCTAC 
GGAGTTCTCT GTGATTCCAA CTCCCGTGTT GTTGCTCTTA ACATCACCGG CAACGGCGGC 
GTCGAAGATG GTAAACTGAT TTCTCATCCT TGCTCTGATT TTTACAAGTT TCCGCTTTAT 
GGGTTTGGAA TTAGGAGAAG TTGTGTTGGT TTTAAGGGTT CTTTGTTTGG AAAGTTTCCA 
TCTTTGATCA GTGAGTTAAC TGAGCTTAGG GTCTTGAGTT TACCCTTTAA TGTGTTGGAG 
GGTTTTATTC CTAAAGAGAT TTGGAATATG GAGAAGCTTG AGGTTCTTGA TTTAGAAGGT 
AATTTGATTA GCGGGTCTAT TCCTTTGGGT TTTGAAGGTT TGAGGAAATT GAGGGTTTTG 
AATTTAGGGT TTAATAAGAT TGTTGGAATG GTGCCTAGTG TTTTAGGTGA TATTGATAGT 
TTGGAGGTTC TGAATTTGGC TGCAAATGGT TTGAATGGTT CTGTTCCTGG TTTTGTTGGG 
AAGTTTAGAG GGGTGTATTT GTCTTTTAAT CAGTTCAGTG GTGTTATTCC GGAGGAGATT 
GGTGAGAATT GTGGGAAGCT TGAGCATTTG GATTTGTCTG GTAATTTGTT GGTTCAAGAG 
ATTCCGAAGA GTTTGGGGAA TTGTGGTGGG TTGAAGACGC TTTTGTTGTA TTCGAATTTG 
TTGGAAGAGG ATATTCCTGC TGAATTTGGG AAGCTTAAGA GTCTTGAGGT GTTGGATGTT 
TCGAGGAATA CACTTAGTGG TCATATTCCT CGTGAGCTTG GGAATTGTAC GGAGCTTTCT 
GTTGTAGTGC TGTCCAACCT TTTCGATCCG GTTGGGGATG GTGAGTTTGT TACTTTGAAT 
GATGAGTTGA ATTATTTTGA AGGTGGAATG CCTGAGGAAG TTGTGTCACT TCCTAAGCTT 
AGAATTTTGT GGGCTCCCAT GGTGAATTTG GAGGGTGGTA TTCCTACGAG TTGGGGTGCT 
TGTGGTAACT TGGAGATGGT CAATTTGGCT CTGAATTTCT TCACCGGAGA GTTCCCAAAC 
CGGCTTGGTT TATGTAAGAA GCTACATTTT CTTGATCTGA GCTCAAACAA TCTTACTGGG 
GAGCTTTCTA AGGAACTTCA TGTTCCATGT ATGTCTGTGT TTGATGTTAG TGCAAACATG 
TTGTCCGGTT CAGTTCCTGA TTTCTCCGAT AATGTTTGTG CTCCCTATCC TTCTCAGAAT 
GGGAATCCAT TTGAAGCTGA CGATGTCATG TCTCCTTATG CATCATATTT TTCATCGAAA 
GCTCATGAGA GAACCATTTA TGCGTCATTA GGGGGAAATG GTCTTTCGGT TTTTCACAAC 
TTTGGGCAAA ATAACTTCAG CGGCATTCAG TCTTTGCCAG TAGTGCGTGA CAGGATGGAG 
GAAAAGAGTA GTTACACACT TCTCGTTGGA GAGAACAAGC TCACGGGACC ATTTCCTACA 
TATTTGTTTG AGAAATGTGA TGGATTAGAT GCATTGCTTT TTAATGTCAG TTATAATCGG 
CTATCTGGTG AGATTCCTTC TAATATTAGT AGCATGTGCA AATCCCTGAA ATTTTTGGAT 
GCATCCAAAA ACCAATTTTC TGGACAAATT CCCTCCACGT TAGGGGATTT GGTCTCTCTT 
GTTTCCCTGA ACCTCAGTAG AAACGGGTTA CAAGGTCAGA TTCCCACCAG CCTTGGACAG 
ATGAAGGTTC TGAAGTTTTT ATCTTTGGCT GGTAATAACT TGAGTGGCTC AATTCCTACC 
AGCCTGGGAC AGATGTACTC TTTACAAGTC TTGGACCTGT CTACAAACTC TCTTACCGGT 
GAGATTCCAA AGTTTATCGA GAACATGAGA AATCTGACCA ATGTTTTGCT GAATAACAAC 
AATCTTTCTG GACACATTCC TGCTGGTTTG GTAAATGTCA CTACACTCTC TGCATTCAAC 
GTGTCATTCA ATAACTTATC TGGATACTTG CCGTCAAATA GTAGCTTGAT CAAATGCAGC 
AGTGCTGTTG GGAATCCATT TTTAAGTTCC TGCCGTGGAC TTTCTTTGAC TGTACCGTCA 
GCAAATCAAC AAGGGCAGGT TGATGAAAGT TCTATGACAT CACAAACTAC TGGCAAAGAT 
AGCAATAATG GTTTCAATGC AATTGAGATA GCATCTATTA CTTCTGCTTC AGCCATTGTT 
TCAGTTCTTA TAGCCCTTAT TGTTCTGTTT TTCATCACAC GCAAGTGGAA GCCGAGGTCC 
AGGGTTGGTG GTTCAGTCAA AAGAGAAGTT ACAGTGTTCA CTGATATCGG CGTCCCATTG 
ACATTTGAAA ATGTTGTCCA AGCCACAGGA AATTTTAATG CAAGCAACTG TATTGGGAGT 
GGAGGATTTG GAGCAACTTA TAAGGCAGAA ATATCACAAG GAATCTTGGT GGCAGTGAAA 
CGTCTTTCCG TTGGACGTTT CCAAGGTGTT CAACAATTCC ATGCAGAGAT TAAGACCCTT 
GGAAGGCTTC ATCATCCTAA CCTCGTCACT TTGATCGGTT ACCATGCTTG TGAGACAGAG 
ATGTTTCTCA TATACAATTA TCTTCCGGGT GGTAATCTTG AAAAGTTCAT CCAGGAGAGA 
TCCACAAGGG CTGTGGATTG GAAAGTTATT CACAAGATTG CACTGGACAT AGCGCGTGCA 
CTTTCCTATC TGCACGATCA ATGTGTACCA CGTGTACTTC ACCGTGATGT CAAGCCTAGC 
AATATCTTGT TGGACGACGA TTGCAATGCT TATCTATCCG ACTTTGGACT CGCCAGACTT 
CTAGGAACTT CAGAGACACA TGCCACTACA GGTGTGGCAG GAACGTTCGG CTATGTTGCT 
CCCGAATATG CGATGACATG TCGTGTTTCT GATAAAGCTG ATGTGTATAG CTACGGTGTA 
GTGCTTCTCG AGTTGCTATC TGATAAGAAA GTGTTGGACC CTTCATTTTC TTCTTATGGG 
AATGGTTTCA ATATAGTTGC ATTTGCGTGC ATGTTACTAA GGCAAGGAAG GGCAAAGGAG 
TTTTTCGCCA CAGGGTTATG GGATGTAGGA CCAGAACATG ATTTGGTAGA GGTTCTACAT 
TTAGCTGTTG TATGTACTGT TGATTCACTT TCAACTAGAC CTACAATGAA ACAGGTTGTG 
AAAAGGTTAA AGCAACTTCA ACCCCCATCA TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013451011.1
CDS	186..3521
Translation	MFSSSIFIKWSFFLLFLSSSNDAVSSFSDKSTLLRFKASLSDPSAVLSTWSSTANHCSFYGVLCDSNSRVVALNITGNGGVEDGKLISHPCSDFYKFPLYGFGIRRSCVGFKGSLFGKFPSLISELTELRVLSLPFNVLEGFIPKEIWNMEKLEVLDLEGNLISGSIPLGFEGLRKLRVLNLGFNKIVGMVPSVLGDIDSLEVLNLAANGLNGSVPGFVGKFRGVYLSFNQFSGVIPEEIGENCGKLEHLDLSGNLLVQEIPKSLGNCGGLKTLLLYSNLLEEDIPAEFGKLKSLEVLDVSRNTLSGHIPRELGNCTELSVVVLSNLFDPVGDGEFVTLNDELNYFEGGMPEEVVSLPKLRILWAPMVNLEGGIPTSWGACGNLEMVNLALNFFTGEFPNRLGLCKKLHFLDLSSNNLTGELSKELHVPCMSVFDVSANMLSGSVPDFSDNVCAPYPSQNGNPFEADDVMSPYASYFSSKAHERTIYASLGGNGLSVFHNFGQNNFSGIQSLPVVRDRMEEKSSYTLLVGENKLTGPFPTYLFEKCDGLDALLFNVSYNRLSGEIPSNISSMCKSLKFLDASKNQFSGQIPSTLGDLVSLVSLNLSRNGLQGQIPTSLGQMKVLKFLSLAGNNLSGSIPTSLGQMYSLQVLDLSTNSLTGEIPKFIENMRNLTNVLLNNNNLSGHIPAGLVNVTTLSAFNVSFNNLSGYLPSNSSLIKCSSAVGNPFLSSCRGLSLTVPSANQQGQVDESSMTSQTTGKDSNNGFNAIEIASITSASAIVSVLIALIVLFFITRKWKPRSRVGGSVKREVTVFTDIGVPLTFENVVQATGNFNASNCIGSGGFGATYKAEISQGILVAVKRLSVGRFQGVQQFHAEIKTLGRLHHPNLVTLIGYHACETEMFLIYNYLPGGNLEKFIQERSTRAVDWKVIHKIALDIARALSYLHDQCVPRVLHRDVKPSNILLDDDCNAYLSDFGLARLLGTSETHATTGVAGTFGYVAPEYAMTCRVSDKADVYSYGVVLLELLSDKKVLDPSFSSYGNGFNIVAFACMLLRQGRAKEFFATGLWDVGPEHDLVEVLHLAVVCTVDSLSTRPTMKQVVKRLKQLQPPSC

Target ORF information:

RefSeq Version	XM_013595557.1
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula LRR receptor-like kinase family protein mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013595557.1

ORF Insert Sequence:

ATGTTTTCAT CTTCAATTTT CATCAAATGG AGTTTCTTTC TTCTCTTCTT ATCTTCTTCA 
AACGACGCCG TTTCGTCATT TTCCGATAAA TCAACTCTCC TCCGCTTCAA AGCCTCTCTT 
TCCGACCCAT CCGCCGTTCT CTCCACCTGG AGCTCTACTG CCAACCACTG TTCCTTCTAC 
GGAGTTCTCT GTGATTCCAA CTCCCGTGTT GTTGCTCTTA ACATCACCGG CAACGGCGGC 
GTCGAAGATG GTAAACTGAT TTCTCATCCT TGCTCTGATT TTTACAAGTT TCCGCTTTAT 
GGGTTTGGAA TTAGGAGAAG TTGTGTTGGT TTTAAGGGTT CTTTGTTTGG AAAGTTTCCA 
TCTTTGATCA GTGAGTTAAC TGAGCTTAGG GTCTTGAGTT TACCCTTTAA TGTGTTGGAG 
GGTTTTATTC CTAAAGAGAT TTGGAATATG GAGAAGCTTG AGGTTCTTGA TTTAGAAGGT 
AATTTGATTA GCGGGTCTAT TCCTTTGGGT TTTGAAGGTT TGAGGAAATT GAGGGTTTTG 
AATTTAGGGT TTAATAAGAT TGTTGGAATG GTGCCTAGTG TTTTAGGTGA TATTGATAGT 
TTGGAGGTTC TGAATTTGGC TGCAAATGGT TTGAATGGTT CTGTTCCTGG TTTTGTTGGG 
AAGTTTAGAG GGGTGTATTT GTCTTTTAAT CAGTTCAGTG GTGTTATTCC GGAGGAGATT 
GGTGAGAATT GTGGGAAGCT TGAGCATTTG GATTTGTCTG GTAATTTGTT GGTTCAAGAG 
ATTCCGAAGA GTTTGGGGAA TTGTGGTGGG TTGAAGACGC TTTTGTTGTA TTCGAATTTG 
TTGGAAGAGG ATATTCCTGC TGAATTTGGG AAGCTTAAGA GTCTTGAGGT GTTGGATGTT 
TCGAGGAATA CACTTAGTGG TCATATTCCT CGTGAGCTTG GGAATTGTAC GGAGCTTTCT 
GTTGTAGTGC TGTCCAACCT TTTCGATCCG GTTGGGGATG GTGAGTTTGT TACTTTGAAT 
GATGAGTTGA ATTATTTTGA AGGTGGAATG CCTGAGGAAG TTGTGTCACT TCCTAAGCTT 
AGAATTTTGT GGGCTCCCAT GGTGAATTTG GAGGGTGGTA TTCCTACGAG TTGGGGTGCT 
TGTGGTAACT TGGAGATGGT CAATTTGGCT CTGAATTTCT TCACCGGAGA GTTCCCAAAC 
CGGCTTGGTT TATGTAAGAA GCTACATTTT CTTGATCTGA GCTCAAACAA TCTTACTGGG 
GAGCTTTCTA AGGAACTTCA TGTTCCATGT ATGTCTGTGT TTGATGTTAG TGCAAACATG 
TTGTCCGGTT CAGTTCCTGA TTTCTCCGAT AATGTTTGTG CTCCCTATCC TTCTCAGAAT 
GGGAATCCAT TTGAAGCTGA CGATGTCATG TCTCCTTATG CATCATATTT TTCATCGAAA 
GCTCATGAGA GAACCATTTA TGCGTCATTA GGGGGAAATG GTCTTTCGGT TTTTCACAAC 
TTTGGGCAAA ATAACTTCAG CGGCATTCAG TCTTTGCCAG TAGTGCGTGA CAGGATGGAG 
GAAAAGAGTA GTTACACACT TCTCGTTGGA GAGAACAAGC TCACGGGACC ATTTCCTACA 
TATTTGTTTG AGAAATGTGA TGGATTAGAT GCATTGCTTT TTAATGTCAG TTATAATCGG 
CTATCTGGTG AGATTCCTTC TAATATTAGT AGCATGTGCA AATCCCTGAA ATTTTTGGAT 
GCATCCAAAA ACCAATTTTC TGGACAAATT CCCTCCACGT TAGGGGATTT GGTCTCTCTT 
GTTTCCCTGA ACCTCAGTAG AAACGGGTTA CAAGGTCAGA TTCCCACCAG CCTTGGACAG 
ATGAAGGTTC TGAAGTTTTT ATCTTTGGCT GGTAATAACT TGAGTGGCTC AATTCCTACC 
AGCCTGGGAC AGATGTACTC TTTACAAGTC TTGGACCTGT CTACAAACTC TCTTACCGGT 
GAGATTCCAA AGTTTATCGA GAACATGAGA AATCTGACCA ATGTTTTGCT GAATAACAAC 
AATCTTTCTG GACACATTCC TGCTGGTTTG GTAAATGTCA CTACACTCTC TGCATTCAAC 
GTGTCATTCA ATAACTTATC TGGATACTTG CCGTCAAATA GTAGCTTGAT CAAATGCAGC 
AGTGCTGTTG GGAATCCATT TTTAAGTTCC TGCCGTGGAC TTTCTTTGAC TGTACCGTCA 
GCAAATCAAC AAGGGCAGGT TGATGAAAGT TCTATGACAT CACAAACTAC TGGCAAAGAT 
AGCAATAATG GTTTCAATGC AATTGAGATA GCATCTATTA CTTCTGCTTC AGCCATTGTT 
TCAGTTCTTA TAGCCCTTAT TGTTCTGTTT TTCATCACAC GCAAGTGGAA GCCGAGGTCC 
AGGGTTGGTG GTTCAGTCAA AAGAGAAGTT ACAGTGTTCA CTGATATCGG CGTCCCATTG 
ACATTTGAAA ATGTTGTCCA AGCCACAGGA AATTTTAATG CAAGCAACTG TATTGGGAGT 
GGAGGATTTG GAGCAACTTA TAAGGCAGAA ATATCACAAG GAATCTTGGT GGCAGTGAAA 
CGTCTTTCCG TTGGACGTTT CCAAGGTGTT CAACAATTCC ATGCAGAGAT TAAGACCCTT 
GGAAGGCTTC ATCATCCTAA CCTCGTCACT TTGATCGGTT ACCATGCTTG TGAGACAGAG 
ATGTTTCTCA TATACAATTA TCTTCCGGGT GGTAATCTTG AAAAGTTCAT CCAGGAGAGA 
TCCACAAGGG CTGTGGATTG GAAAGTTATT CACAAGATTG CACTGGACAT AGCGCGTGCA 
CTTTCCTATC TGCACGATCA ATGTGTACCA CGTGTACTTC ACCGTGATGT CAAGCCTAGC 
AATATCTTGT TGGACGACGA TTGCAATGCT TATCTATCCG ACTTTGGACT CGCCAGACTT 
CTAGGAACTT CAGAGACACA TGCCACTACA GGTGTGGCAG GAACGTTCGG CTATGTTGCT 
CCCGAATATG CGATGACATG TCGTGTTTCT GATAAAGCTG ATGTGTATAG CTACGGTGTA 
GTGCTTCTCG AGTTGCTATC TGATAAGAAA GTGTTGGACC CTTCATTTTC TTCTTATGGG 
AATGGTTTCA ATATAGTTGC ATTTGCGTGC ATGTTACTAA GGCAAGGAAG GGCAAAGGAG 
TTTTTCGCCA CAGGGTTATG GGATGTAGGA CCAGAACATG ATTTGGTAGA GGTTCTACAT 
TTAGCTGTTG TATGTACTGT TGATTCACTT TCAACTAGAC CTACAATGAA ACAGGTTGTG 
AAAAGGTTAA AGCAACTTCA ACCCCCATCA TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC25495342 ( XM_013595557.2 ) cDNA ORF clone, Medicago truncatula(barrel medic) -> XP_013451011.1 Medicago truncatula LRR receptor-like serine/threonine-protein kinase RPK2 (LOC25495342), mRNA.

Price & Availability↑

CloneID	OMe175260
Clone ID Related Accession (Same CDS sequence)	XM_013595557.1 , XM_013595557.2
Accession Version	XM_013595557.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3336bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-22
Organism	Medicago truncatula(barrel medic)
Product	LRR receptor-like serine/threonine-protein kinase RPK2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_016412.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Apr 23, 2018 this sequence version replaced XM_013595557.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Medicago truncatula Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTCAT CTTCAATTTT CATCAAATGG AGTTTCTTTC TTCTCTTCTT ATCTTCTTCA 
AACGACGCCG TTTCGTCATT TTCCGATAAA TCAACTCTCC TCCGCTTCAA AGCCTCTCTT 
TCCGACCCAT CCGCCGTTCT CTCCACCTGG AGCTCTACTG CCAACCACTG TTCCTTCTAC 
GGAGTTCTCT GTGATTCCAA CTCCCGTGTT GTTGCTCTTA ACATCACCGG CAACGGCGGC 
GTCGAAGATG GTAAACTGAT TTCTCATCCT TGCTCTGATT TTTACAAGTT TCCGCTTTAT 
GGGTTTGGAA TTAGGAGAAG TTGTGTTGGT TTTAAGGGTT CTTTGTTTGG AAAGTTTCCA 
TCTTTGATCA GTGAGTTAAC TGAGCTTAGG GTCTTGAGTT TACCCTTTAA TGTGTTGGAG 
GGTTTTATTC CTAAAGAGAT TTGGAATATG GAGAAGCTTG AGGTTCTTGA TTTAGAAGGT 
AATTTGATTA GCGGGTCTAT TCCTTTGGGT TTTGAAGGTT TGAGGAAATT GAGGGTTTTG 
AATTTAGGGT TTAATAAGAT TGTTGGAATG GTGCCTAGTG TTTTAGGTGA TATTGATAGT 
TTGGAGGTTC TGAATTTGGC TGCAAATGGT TTGAATGGTT CTGTTCCTGG TTTTGTTGGG 
AAGTTTAGAG GGGTGTATTT GTCTTTTAAT CAGTTCAGTG GTGTTATTCC GGAGGAGATT 
GGTGAGAATT GTGGGAAGCT TGAGCATTTG GATTTGTCTG GTAATTTGTT GGTTCAAGAG 
ATTCCGAAGA GTTTGGGGAA TTGTGGTGGG TTGAAGACGC TTTTGTTGTA TTCGAATTTG 
TTGGAAGAGG ATATTCCTGC TGAATTTGGG AAGCTTAAGA GTCTTGAGGT GTTGGATGTT 
TCGAGGAATA CACTTAGTGG TCATATTCCT CGTGAGCTTG GGAATTGTAC GGAGCTTTCT 
GTTGTAGTGC TGTCCAACCT TTTCGATCCG GTTGGGGATG GTGAGTTTGT TACTTTGAAT 
GATGAGTTGA ATTATTTTGA AGGTGGAATG CCTGAGGAAG TTGTGTCACT TCCTAAGCTT 
AGAATTTTGT GGGCTCCCAT GGTGAATTTG GAGGGTGGTA TTCCTACGAG TTGGGGTGCT 
TGTGGTAACT TGGAGATGGT CAATTTGGCT CTGAATTTCT TCACCGGAGA GTTCCCAAAC 
CGGCTTGGTT TATGTAAGAA GCTACATTTT CTTGATCTGA GCTCAAACAA TCTTACTGGG 
GAGCTTTCTA AGGAACTTCA TGTTCCATGT ATGTCTGTGT TTGATGTTAG TGCAAACATG 
TTGTCCGGTT CAGTTCCTGA TTTCTCCGAT AATGTTTGTG CTCCCTATCC TTCTCAGAAT 
GGGAATCCAT TTGAAGCTGA CGATGTCATG TCTCCTTATG CATCATATTT TTCATCGAAA 
GCTCATGAGA GAACCATTTA TGCGTCATTA GGGGGAAATG GTCTTTCGGT TTTTCACAAC 
TTTGGGCAAA ATAACTTCAG CGGCATTCAG TCTTTGCCAG TAGTGCGTGA CAGGATGGAG 
GAAAAGAGTA GTTACACACT TCTCGTTGGA GAGAACAAGC TCACGGGACC ATTTCCTACA 
TATTTGTTTG AGAAATGTGA TGGATTAGAT GCATTGCTTT TTAATGTCAG TTATAATCGG 
CTATCTGGTG AGATTCCTTC TAATATTAGT AGCATGTGCA AATCCCTGAA ATTTTTGGAT 
GCATCCAAAA ACCAATTTTC TGGACAAATT CCCTCCACGT TAGGGGATTT GGTCTCTCTT 
GTTTCCCTGA ACCTCAGTAG AAACGGGTTA CAAGGTCAGA TTCCCACCAG CCTTGGACAG 
ATGAAGGTTC TGAAGTTTTT ATCTTTGGCT GGTAATAACT TGAGTGGCTC AATTCCTACC 
AGCCTGGGAC AGATGTACTC TTTACAAGTC TTGGACCTGT CTACAAACTC TCTTACCGGT 
GAGATTCCAA AGTTTATCGA GAACATGAGA AATCTGACCA ATGTTTTGCT GAATAACAAC 
AATCTTTCTG GACACATTCC TGCTGGTTTG GTAAATGTCA CTACACTCTC TGCATTCAAC 
GTGTCATTCA ATAACTTATC TGGATACTTG CCGTCAAATA GTAGCTTGAT CAAATGCAGC 
AGTGCTGTTG GGAATCCATT TTTAAGTTCC TGCCGTGGAC TTTCTTTGAC TGTACCGTCA 
GCAAATCAAC AAGGGCAGGT TGATGAAAGT TCTATGACAT CACAAACTAC TGGCAAAGAT 
AGCAATAATG GTTTCAATGC AATTGAGATA GCATCTATTA CTTCTGCTTC AGCCATTGTT 
TCAGTTCTTA TAGCCCTTAT TGTTCTGTTT TTCATCACAC GCAAGTGGAA GCCGAGGTCC 
AGGGTTGGTG GTTCAGTCAA AAGAGAAGTT ACAGTGTTCA CTGATATCGG CGTCCCATTG 
ACATTTGAAA ATGTTGTCCA AGCCACAGGA AATTTTAATG CAAGCAACTG TATTGGGAGT 
GGAGGATTTG GAGCAACTTA TAAGGCAGAA ATATCACAAG GAATCTTGGT GGCAGTGAAA 
CGTCTTTCCG TTGGACGTTT CCAAGGTGTT CAACAATTCC ATGCAGAGAT TAAGACCCTT 
GGAAGGCTTC ATCATCCTAA CCTCGTCACT TTGATCGGTT ACCATGCTTG TGAGACAGAG 
ATGTTTCTCA TATACAATTA TCTTCCGGGT GGTAATCTTG AAAAGTTCAT CCAGGAGAGA 
TCCACAAGGG CTGTGGATTG GAAAGTTATT CACAAGATTG CACTGGACAT AGCGCGTGCA 
CTTTCCTATC TGCACGATCA ATGTGTACCA CGTGTACTTC ACCGTGATGT CAAGCCTAGC 
AATATCTTGT TGGACGACGA TTGCAATGCT TATCTATCCG ACTTTGGACT CGCCAGACTT 
CTAGGAACTT CAGAGACACA TGCCACTACA GGTGTGGCAG GAACGTTCGG CTATGTTGCT 
CCCGAATATG CGATGACATG TCGTGTTTCT GATAAAGCTG ATGTGTATAG CTACGGTGTA 
GTGCTTCTCG AGTTGCTATC TGATAAGAAA GTGTTGGACC CTTCATTTTC TTCTTATGGG 
AATGGTTTCA ATATAGTTGC ATTTGCGTGC ATGTTACTAA GGCAAGGAAG GGCAAAGGAG 
TTTTTCGCCA CAGGGTTATG GGATGTAGGA CCAGAACATG ATTTGGTAGA GGTTCTACAT 
TTAGCTGTTG TATGTACTGT TGATTCACTT TCAACTAGAC CTACAATGAA ACAGGTTGTG 
AAAAGGTTAA AGCAACTTCA ACCCCCATCA TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013451011.1
CDS	186..3521
Translation	MFSSSIFIKWSFFLLFLSSSNDAVSSFSDKSTLLRFKASLSDPSAVLSTWSSTANHCSFYGVLCDSNSRVVALNITGNGGVEDGKLISHPCSDFYKFPLYGFGIRRSCVGFKGSLFGKFPSLISELTELRVLSLPFNVLEGFIPKEIWNMEKLEVLDLEGNLISGSIPLGFEGLRKLRVLNLGFNKIVGMVPSVLGDIDSLEVLNLAANGLNGSVPGFVGKFRGVYLSFNQFSGVIPEEIGENCGKLEHLDLSGNLLVQEIPKSLGNCGGLKTLLLYSNLLEEDIPAEFGKLKSLEVLDVSRNTLSGHIPRELGNCTELSVVVLSNLFDPVGDGEFVTLNDELNYFEGGMPEEVVSLPKLRILWAPMVNLEGGIPTSWGACGNLEMVNLALNFFTGEFPNRLGLCKKLHFLDLSSNNLTGELSKELHVPCMSVFDVSANMLSGSVPDFSDNVCAPYPSQNGNPFEADDVMSPYASYFSSKAHERTIYASLGGNGLSVFHNFGQNNFSGIQSLPVVRDRMEEKSSYTLLVGENKLTGPFPTYLFEKCDGLDALLFNVSYNRLSGEIPSNISSMCKSLKFLDASKNQFSGQIPSTLGDLVSLVSLNLSRNGLQGQIPTSLGQMKVLKFLSLAGNNLSGSIPTSLGQMYSLQVLDLSTNSLTGEIPKFIENMRNLTNVLLNNNNLSGHIPAGLVNVTTLSAFNVSFNNLSGYLPSNSSLIKCSSAVGNPFLSSCRGLSLTVPSANQQGQVDESSMTSQTTGKDSNNGFNAIEIASITSASAIVSVLIALIVLFFITRKWKPRSRVGGSVKREVTVFTDIGVPLTFENVVQATGNFNASNCIGSGGFGATYKAEISQGILVAVKRLSVGRFQGVQQFHAEIKTLGRLHHPNLVTLIGYHACETEMFLIYNYLPGGNLEKFIQERSTRAVDWKVIHKIALDIARALSYLHDQCVPRVLHRDVKPSNILLDDDCNAYLSDFGLARLLGTSETHATTGVAGTFGYVAPEYAMTCRVSDKADVYSYGVVLLELLSDKKVLDPSFSSYGNGFNIVAFACMLLRQGRAKEFFATGLWDVGPEHDLVEVLHLAVVCTVDSLSTRPTMKQVVKRLKQLQPPSC

Target ORF information:

RefSeq Version	XM_013595557.2
Organism	Medicago truncatula(barrel medic)
Definition	Medicago truncatula LRR receptor-like serine/threonine-protein kinase RPK2 (LOC25495342), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_013595557.2

ORF Insert Sequence:

ATGTTTTCAT CTTCAATTTT CATCAAATGG AGTTTCTTTC TTCTCTTCTT ATCTTCTTCA 
AACGACGCCG TTTCGTCATT TTCCGATAAA TCAACTCTCC TCCGCTTCAA AGCCTCTCTT 
TCCGACCCAT CCGCCGTTCT CTCCACCTGG AGCTCTACTG CCAACCACTG TTCCTTCTAC 
GGAGTTCTCT GTGATTCCAA CTCCCGTGTT GTTGCTCTTA ACATCACCGG CAACGGCGGC 
GTCGAAGATG GTAAACTGAT TTCTCATCCT TGCTCTGATT TTTACAAGTT TCCGCTTTAT 
GGGTTTGGAA TTAGGAGAAG TTGTGTTGGT TTTAAGGGTT CTTTGTTTGG AAAGTTTCCA 
TCTTTGATCA GTGAGTTAAC TGAGCTTAGG GTCTTGAGTT TACCCTTTAA TGTGTTGGAG 
GGTTTTATTC CTAAAGAGAT TTGGAATATG GAGAAGCTTG AGGTTCTTGA TTTAGAAGGT 
AATTTGATTA GCGGGTCTAT TCCTTTGGGT TTTGAAGGTT TGAGGAAATT GAGGGTTTTG 
AATTTAGGGT TTAATAAGAT TGTTGGAATG GTGCCTAGTG TTTTAGGTGA TATTGATAGT 
TTGGAGGTTC TGAATTTGGC TGCAAATGGT TTGAATGGTT CTGTTCCTGG TTTTGTTGGG 
AAGTTTAGAG GGGTGTATTT GTCTTTTAAT CAGTTCAGTG GTGTTATTCC GGAGGAGATT 
GGTGAGAATT GTGGGAAGCT TGAGCATTTG GATTTGTCTG GTAATTTGTT GGTTCAAGAG 
ATTCCGAAGA GTTTGGGGAA TTGTGGTGGG TTGAAGACGC TTTTGTTGTA TTCGAATTTG 
TTGGAAGAGG ATATTCCTGC TGAATTTGGG AAGCTTAAGA GTCTTGAGGT GTTGGATGTT 
TCGAGGAATA CACTTAGTGG TCATATTCCT CGTGAGCTTG GGAATTGTAC GGAGCTTTCT 
GTTGTAGTGC TGTCCAACCT TTTCGATCCG GTTGGGGATG GTGAGTTTGT TACTTTGAAT 
GATGAGTTGA ATTATTTTGA AGGTGGAATG CCTGAGGAAG TTGTGTCACT TCCTAAGCTT 
AGAATTTTGT GGGCTCCCAT GGTGAATTTG GAGGGTGGTA TTCCTACGAG TTGGGGTGCT 
TGTGGTAACT TGGAGATGGT CAATTTGGCT CTGAATTTCT TCACCGGAGA GTTCCCAAAC 
CGGCTTGGTT TATGTAAGAA GCTACATTTT CTTGATCTGA GCTCAAACAA TCTTACTGGG 
GAGCTTTCTA AGGAACTTCA TGTTCCATGT ATGTCTGTGT TTGATGTTAG TGCAAACATG 
TTGTCCGGTT CAGTTCCTGA TTTCTCCGAT AATGTTTGTG CTCCCTATCC TTCTCAGAAT 
GGGAATCCAT TTGAAGCTGA CGATGTCATG TCTCCTTATG CATCATATTT TTCATCGAAA 
GCTCATGAGA GAACCATTTA TGCGTCATTA GGGGGAAATG GTCTTTCGGT TTTTCACAAC 
TTTGGGCAAA ATAACTTCAG CGGCATTCAG TCTTTGCCAG TAGTGCGTGA CAGGATGGAG 
GAAAAGAGTA GTTACACACT TCTCGTTGGA GAGAACAAGC TCACGGGACC ATTTCCTACA 
TATTTGTTTG AGAAATGTGA TGGATTAGAT GCATTGCTTT TTAATGTCAG TTATAATCGG 
CTATCTGGTG AGATTCCTTC TAATATTAGT AGCATGTGCA AATCCCTGAA ATTTTTGGAT 
GCATCCAAAA ACCAATTTTC TGGACAAATT CCCTCCACGT TAGGGGATTT GGTCTCTCTT 
GTTTCCCTGA ACCTCAGTAG AAACGGGTTA CAAGGTCAGA TTCCCACCAG CCTTGGACAG 
ATGAAGGTTC TGAAGTTTTT ATCTTTGGCT GGTAATAACT TGAGTGGCTC AATTCCTACC 
AGCCTGGGAC AGATGTACTC TTTACAAGTC TTGGACCTGT CTACAAACTC TCTTACCGGT 
GAGATTCCAA AGTTTATCGA GAACATGAGA AATCTGACCA ATGTTTTGCT GAATAACAAC 
AATCTTTCTG GACACATTCC TGCTGGTTTG GTAAATGTCA CTACACTCTC TGCATTCAAC 
GTGTCATTCA ATAACTTATC TGGATACTTG CCGTCAAATA GTAGCTTGAT CAAATGCAGC 
AGTGCTGTTG GGAATCCATT TTTAAGTTCC TGCCGTGGAC TTTCTTTGAC TGTACCGTCA 
GCAAATCAAC AAGGGCAGGT TGATGAAAGT TCTATGACAT CACAAACTAC TGGCAAAGAT 
AGCAATAATG GTTTCAATGC AATTGAGATA GCATCTATTA CTTCTGCTTC AGCCATTGTT 
TCAGTTCTTA TAGCCCTTAT TGTTCTGTTT TTCATCACAC GCAAGTGGAA GCCGAGGTCC 
AGGGTTGGTG GTTCAGTCAA AAGAGAAGTT ACAGTGTTCA CTGATATCGG CGTCCCATTG 
ACATTTGAAA ATGTTGTCCA AGCCACAGGA AATTTTAATG CAAGCAACTG TATTGGGAGT 
GGAGGATTTG GAGCAACTTA TAAGGCAGAA ATATCACAAG GAATCTTGGT GGCAGTGAAA 
CGTCTTTCCG TTGGACGTTT CCAAGGTGTT CAACAATTCC ATGCAGAGAT TAAGACCCTT 
GGAAGGCTTC ATCATCCTAA CCTCGTCACT TTGATCGGTT ACCATGCTTG TGAGACAGAG 
ATGTTTCTCA TATACAATTA TCTTCCGGGT GGTAATCTTG AAAAGTTCAT CCAGGAGAGA 
TCCACAAGGG CTGTGGATTG GAAAGTTATT CACAAGATTG CACTGGACAT AGCGCGTGCA 
CTTTCCTATC TGCACGATCA ATGTGTACCA CGTGTACTTC ACCGTGATGT CAAGCCTAGC 
AATATCTTGT TGGACGACGA TTGCAATGCT TATCTATCCG ACTTTGGACT CGCCAGACTT 
CTAGGAACTT CAGAGACACA TGCCACTACA GGTGTGGCAG GAACGTTCGG CTATGTTGCT 
CCCGAATATG CGATGACATG TCGTGTTTCT GATAAAGCTG ATGTGTATAG CTACGGTGTA 
GTGCTTCTCG AGTTGCTATC TGATAAGAAA GTGTTGGACC CTTCATTTTC TTCTTATGGG 
AATGGTTTCA ATATAGTTGC ATTTGCGTGC ATGTTACTAA GGCAAGGAAG GGCAAAGGAG 
TTTTTCGCCA CAGGGTTATG GGATGTAGGA CCAGAACATG ATTTGGTAGA GGTTCTACAT 
TTAGCTGTTG TATGTACTGT TGATTCACTT TCAACTAGAC CTACAATGAA ACAGGTTGTG 
AAAAGGTTAA AGCAACTTCA ACCCCCATCA TGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

An improved genome release (version Mt4.0) for the model legume Medicago truncatula.
BMC genomics15312(2014 Apr)
Tang H,Krishnakumar V,Bidwell S,Rosen B,Chan A,Zhou S,Gentzbittel L,Childs KL,Yandell M,Gundlach H,Mayer KF,Schwartz DC,Town CD

The Medicago genome provides insight into the evolution of rhizobial symbioses.
Nature480(7378)520-4(2011 Nov)
Young ND,Debell? F,Oldroyd GE,Geurts R,Cannon SB,Udvardi MK,Benedito VA,Mayer KF,Gouzy J,Schoof H,Van de Peer Y,Proost S,Cook DR,Meyers BC,Spannagl M,Cheung F,De Mita S,Krishnakumar V,Gundlach H,Zhou S,Mudge J,Bharti AK,Murray JD,Naoumkina MA,Rosen B,Silverstein KA,Tang H,Rombauts S,Zhao PX,Zhou P,Barbe V,Bardou P,Bechner M,Bellec A,Berger A,Berg?s H,Bidwell S,Bisseling T,Choisne N,Couloux A,Denny R,Deshpande S,Dai X,Doyle JJ,Dudez AM,Farmer AD,Fouteau S,Franken C,Gibelin C,Gish J,Goldstein S,Gonz?lez AJ,Green PJ,Hallab A,Hartog M,Hua A,Humphray SJ,Jeong DH,Jing Y,J?cker A,Kenton SM,Kim DJ,Klee K,Lai H,Lang C,Lin S,Macmil SL,Magdelenat G,Matthews L,McCorrison J,Monaghan EL,Mun JH,Najar FZ,Nicholson C,Noirot C,O'Bleness M,Paule CR,Poulain J,Prion F,Qin B,Qu C,Retzel EF,Riddle C,Sallet E,Samain S,Samson N,Sanders I,Saurat O,Scarpelli C,Schiex T,Segurens B,Severin AJ,Sherrier DJ,Shi R,Sims S,Singer SR,Sinharoy S,Sterck L,Viollet A,Wang BB,Wang K,Wang M,Wang X,Warfsmann J,Weissenbach J,White DD,White JD,Wiley GB,Wincker P,Xing Y,Yang L,Yao Z,Ying F,Zhai J,Zhou L,Zuber A,D?nari? J,Dixon RA,May GD,Schwartz DC,Rogers J,Qu?tier F,Town CD,Roe BA

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LOC25495342 cDNA ORF clone, Medicago truncatula(barrel medic)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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