MGG_11917 cDNA ORF clone, Pyricularia oryzae 70-15

The following MGG_11917 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MGG_11917 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMe08865 XM_003709127.1
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Pyricularia oryzae 70-15 uncharacterized protein (MGG_11917), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMe08865
    Clone ID Related Accession (Same CDS sequence) XM_003709127.1
    Accession Version XM_003709127.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4332bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-28
    Organism Pyricularia oryzae 70-15
    Product uncharacterized protein
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_017844).

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCCAGCA CGTCACCCCA GCCTGGAAGT ACCGCAATAC CACATCCACA ACCACCACCT 
    CCACTCAAGG AGCCGACAAA CCCAGTTCAG GTATCGTCGT GTGGTAACCC TGTCGATCCA
    GCACAAGTCC TGTCGCAGGG GCCGCCAGAC CGCAGCCGTA TTCCTGTCTC AGCGGCAGAG
    GCAGAAAAGA CAAGCAATGG CACGAGCGCA AGCACGACCG CAGGCACAGG CACAGGCACA
    GCTGCTGCAA CGATAAACGC TACACCTCCA CCACTCCCCC CGGGTGTAGA TGCTGCTTGT
    CCTTCCACCC CTGCGCAGAA CCAGCAGCAT GGCGCCGGTA ATGCGGACCA GAATAAAAAG
    CCCCGAGGCC CAGTCTTACC TCGACCTACT GGCCCGTCCC TGCTGACGCA AGCCTTGGCT
    TCCGCCCGTG GTATACTCTC TAATACCTCG ACCCCCGTTA TTGCCCAGCC GGATCCGAAC
    CGTGTCCTGT CACATAGTCC CGGCAACCAG GGTCCTACCT CTCCCTCTAC TACTTCTTCT
    TCTTCCCCTC TGCACCAACC ACACAACGCC CCTCCAACGC TACCCCCAGG TTCATCTTAC
    ACCTCTCCTT CGCCCACCGC TACTCCAATC TCGACGTCAC CCCACAAAAC CAATGATGCC
    CGCAGCGATG CCGCACCGCA TGGGCTCAGC AACTCTTTAA CGACGCAAAC TGGCCTCCCA
    CCCCGATCGT CAGTCATGTC GGCCTCGACC GTGGTTTCTA CCCTCACCGT CTCCGGCTCG
    CTTCCCGTAG CTAACCCAAA GCTGCACCTA CACGTTGGTT ACAATGTCCT GGATTCGAGC
    GGGTTAGATG CCAGAAGTCG GGCCATCAAT GGCCGCCGGC CGAAAAACGC CGACAGGGTT
    GGGTCTTCTG ATTACCCGGA CTCGACGCAT CTTTCATTGA GAGCTCCGAG TGCCCCCACT
    TTGAGTTCTT CAGCAGGCGC AAAGCCGCAG TCGACATCGC CCAGCGTACA CCCTCGGCCA
    AACTTGACTA CCAAGCAAGG AGCAGACTCG GCTTCGGTCG ACGATCGGAA TCTTACAAGG
    AAGCAAAGTA TGGAACATAA GGTATCTGTC GGACCAGAAA AGATCTGGTC TATAGGTAGT
    GGCGAGTCGG ATTGCCTCGA GGATGGACAA GTAGAGAAGT CGGTGACTGA AGTCATATCC
    GGCGTCGAGC ACAACAATCG CAGCCGCAAA GCTAGTCATA GTCTCCGCTT TTTCAAGGAA
    GGACTACCAG AAGAGAGAAA CAAGAAGAAA GATTCTTCTA AACATACCGG TCATGTATGG
    GATACTACAT CCCCGAGTCT TGAGGCCCCA GCAACAATTC CGGAACATTC TGTCGCTGAA
    AGCGATTCAG CCCTTTCATC GTCACCAAAG TCTGTGGATC CTCATGCTGA TATATTTCAC
    CAAATTCAAC GTTCGACCTC GGTCAAGAAG GCCAAGGAGA AAGATGCCAT CAAGAAGGGA
    TCTGGTAATG ATTATTTTGC AGACGTCGAG CCGGGCTTCA CGTCCAAGTT CGCAGATCGC
    CGCCAAGACA TCCCACAGCC CAAGGAATAT GAAGTGCTGC CCGATCCTGC ACTTGAGCTT
    GAGAAGGAGG TGCCTCGTGA AAGGAGGAAG TCTAGCGACA GCATCTTGTC TACAAGGAGT
    ACCGACGATG GAGATGACTC GGGCGAGGAG AAGATATCCT CTGCGGTCTT CGTGCCCCAC
    CAAGCTCCAC AGGAACAGAT GGGAACTCCT GCAGACCCCG TGAGTATCCC GGGTCAAGCT
    AATGGGCAGA GGATATCGCC CAGAGCCGAC GGTTGGCTCG TCAAGGCTGA CGAGCCAGAA
    GTCGATCGTC GCGATGAAGC TGGTGATGAG TCAGGGGCAC TCACTTTGTC GGGGTCAGAC
    CGAACTTCTG TTACTCAAGG ATCCGGTCTA GAGCAGCCCG TCTCGAGGTT GCCCCCGCAA
    GGAGCCTCTG CTATCATAGA CTTCGCAGCA CCTAGAGGTG ATCTGAACGG GCCATCTCAG
    CAACTGCAAA CTGCAGTAAC CTATGAAGAT CAACCATCCC CGCAACCTTT GGAGGCGATT
    GAGTTGATTC CTTACAAACA CCAAGTCGGT GGTCACACAA CACTCTGGCG ATTCTCAAAG
    CGTGCAGTTT GCAAACAATT GAACAACCGG GAGAATGAGT TCTATGAGAG GATTGAGCGA
    GATGTTAGGG ATCTCCTCGC TTTCCTTCCT AGGTACATTG GGGTTCTCAA CGTGACTTTC
    AAAAAGCAGC CACGCCGCAA ATCCACGCTT AAGAGAGAGG ATACGGCTGC TTTAGAGCGA
    AAGCTGCTTG ACCAGAGCGA TTTGAAAAAG GCCCTAGGGA GCAATGGAGC TGTTGGTCAA
    CATGAAGCCG TGTCTAGCCC AGCTGAGGAT ACAAATGCGG CCCCTACGCA CACTAGAGTA
    ATCAGTCAAT CTCTGCAATC TAGTCAAATT CCGGTGCCTA CTGTCACTTT TGTTGATAAT
    CAACATATTC TGCCGCGCAA TCTGCTGCAG CCTTCGCCAA GTCCGACTAT CGAGGTCGGT
    AGCAGCCGCG TTAGAAGCAG CTCGGCTGTC ACCGTGCCAG GTTGTAGTCC GGATGGGCGG
    AAAAAGGCCG CGAGCCGGCC AACGCTTGAA GATCGACATG CGAACAGCTG GGGTGCCACA
    ACGGTCAACA AGGATTTGCG GAACAAGGTT TTCAACGACG TTTTCATGAA GACACCTTTT
    CCAATGCGGC CACACCGCAG GTGGCACTCG CAGCGTGCAC CCCTTCCAAG GTCCTCACTG
    CCGAACTTTT TGGTGCCTGC AGGCAGTGTT GAGGACAGGG CAGAATCCGA GCCTGATCTT
    CCGCCACGCC GCTCGAGCAC TGGTCAAGCC AGCTCTCGGT TACGCAACAG CTTGAGCGAT
    ATGGGGCCCG AAATTGATCA TTCAGAGAGC ACATCGGTGC CGAAGAACGT CCAGGACAGA
    CTCAAAGCAG AGGAAGATGT CGGACCTAAG GACATTACGG GCACAAGTGC ACCAGAGCCC
    GAGATTCCTG TCGAGAAGGG AGCTTCTCAG GGTAAACGTC GTCGGTATTC TGGTACGGCT
    TTGCGCAGGA GGGCCAAAGA GGGGTTGGAT TCATCTGAGA GGAAACTGCA ATACTTTCAA
    GAGCCCGACG ATGAGGACTT CAAACGGGAA ACTGATTTTG ATACCGGATT GGTTCCTGCA
    GCAGTGGAGG GAAACTCACA AAAAAACCCA ACACAAAACG ATAACGGGCG CCCCAGGAGC
    ACAGACTCAG ATGCAGGACC GGCGACTTTG CATCTTGAGC CAGAAGAGAC GCCTGTCACG
    TCCCCCGGCT GGACTATAAG CAATTACCCT TCAACAGTTA CATCGGGGGC CGCCAGCCCA
    TCGACCGAGT TCAAGCGAAT ATCGCGACCC GTCAACCCCA AGGAGGCCCG CGTGGCCGAT
    TCGCGCGTGG AATACTTCCT CCTGCTTGAG GACTTGACCG CAGGCATGAA ACGGCCATGT
    ATCATGGACC TCAAGATGGG GACCCGTCAA TACGGAGTCG ACGCGACCCC TAAGAAGCAG
    GCTTCACAGC AACGCAAGTG CGCAGCCACC ACGTCGCGGA GCCTCGGCGT ACGGGTCTGC
    GGGTTGCAAG TGTGGGACGC AGCGCAACAG ACATATATAT TTCGAGATAA GTACTATGGC
    CGGGACCTCA GACCTGGGGC CGAATTCCAG GAGGCGTTGA CGCGATTCCT GTACGACGGT
    GTGGATCCGG CGAGCATCCT TCGACACATA CCCACCGTCC TGTCCAAGCT TAGAGAGTTG
    GAGGTCATCA TTGAAAAGCT AGACGACTAC AGATTCTATG CGGCCAGCCT ATTGATGTTT
    TATGACGGAG ATCGGAGCGG CGATGACGGG AACGAGACCG CGATCGAGGA TTCGACTACG
    GACTTTGCCA CGGACACTGA AGAGGGACGC ACGGCTGCCT CGGTTGCCGC GCGCAAGAGT
    CGCAAGCGTA ACAAGCGCGA GATTGATTTC AAGATTGCCG ACTTTGCCAA CGGAGTCACA
    GCCTTGTCCG GTGGTTATGG GGCCGAGGAC AGGCCCTGTC CGCCACAGCA CCCCGGCCAG
    CCCGACCGCG GCTTTCTGAG AGGCCTAAGA AGTCTCCGGC GCTACTTTTT GAAGATCCAG
    AGGGAGACGA GGGAGCAGCT CGGACTCGGG CTTCTTGGCA GTCGTCACGG AGTCAATTAC
    GATGGCGCGG CCGACAGCGG CTTTGGTGGC GATGATGGAT GGGATGAGGA CGAGGGTTCT
    GTGAGCGAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003709175.1
    CDS361..4692
    Translation

    Target ORF information:

    RefSeq Version XM_003709127.1
    Organism Pyricularia oryzae 70-15
    Definition Pyricularia oryzae 70-15 uncharacterized protein (MGG_11917), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003709127.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGTCCAGCA CGTCACCCCA GCCTGGAAGT ACCGCAATAC CACATCCACA ACCACCACCT 
    CCACTCAAGG AGCCGACAAA CCCAGTTCAG GTATCGTCGT GTGGTAACCC TGTCGATCCA
    GCACAAGTCC TGTCGCAGGG GCCGCCAGAC CGCAGCCGTA TTCCTGTCTC AGCGGCAGAG
    GCAGAAAAGA CAAGCAATGG CACGAGCGCA AGCACGACCG CAGGCACAGG CACAGGCACA
    GCTGCTGCAA CGATAAACGC TACACCTCCA CCACTCCCCC CGGGTGTAGA TGCTGCTTGT
    CCTTCCACCC CTGCGCAGAA CCAGCAGCAT GGCGCCGGTA ATGCGGACCA GAATAAAAAG
    CCCCGAGGCC CAGTCTTACC TCGACCTACT GGCCCGTCCC TGCTGACGCA AGCCTTGGCT
    TCCGCCCGTG GTATACTCTC TAATACCTCG ACCCCCGTTA TTGCCCAGCC GGATCCGAAC
    CGTGTCCTGT CACATAGTCC CGGCAACCAG GGTCCTACCT CTCCCTCTAC TACTTCTTCT
    TCTTCCCCTC TGCACCAACC ACACAACGCC CCTCCAACGC TACCCCCAGG TTCATCTTAC
    ACCTCTCCTT CGCCCACCGC TACTCCAATC TCGACGTCAC CCCACAAAAC CAATGATGCC
    CGCAGCGATG CCGCACCGCA TGGGCTCAGC AACTCTTTAA CGACGCAAAC TGGCCTCCCA
    CCCCGATCGT CAGTCATGTC GGCCTCGACC GTGGTTTCTA CCCTCACCGT CTCCGGCTCG
    CTTCCCGTAG CTAACCCAAA GCTGCACCTA CACGTTGGTT ACAATGTCCT GGATTCGAGC
    GGGTTAGATG CCAGAAGTCG GGCCATCAAT GGCCGCCGGC CGAAAAACGC CGACAGGGTT
    GGGTCTTCTG ATTACCCGGA CTCGACGCAT CTTTCATTGA GAGCTCCGAG TGCCCCCACT
    TTGAGTTCTT CAGCAGGCGC AAAGCCGCAG TCGACATCGC CCAGCGTACA CCCTCGGCCA
    AACTTGACTA CCAAGCAAGG AGCAGACTCG GCTTCGGTCG ACGATCGGAA TCTTACAAGG
    AAGCAAAGTA TGGAACATAA GGTATCTGTC GGACCAGAAA AGATCTGGTC TATAGGTAGT
    GGCGAGTCGG ATTGCCTCGA GGATGGACAA GTAGAGAAGT CGGTGACTGA AGTCATATCC
    GGCGTCGAGC ACAACAATCG CAGCCGCAAA GCTAGTCATA GTCTCCGCTT TTTCAAGGAA
    GGACTACCAG AAGAGAGAAA CAAGAAGAAA GATTCTTCTA AACATACCGG TCATGTATGG
    GATACTACAT CCCCGAGTCT TGAGGCCCCA GCAACAATTC CGGAACATTC TGTCGCTGAA
    AGCGATTCAG CCCTTTCATC GTCACCAAAG TCTGTGGATC CTCATGCTGA TATATTTCAC
    CAAATTCAAC GTTCGACCTC GGTCAAGAAG GCCAAGGAGA AAGATGCCAT CAAGAAGGGA
    TCTGGTAATG ATTATTTTGC AGACGTCGAG CCGGGCTTCA CGTCCAAGTT CGCAGATCGC
    CGCCAAGACA TCCCACAGCC CAAGGAATAT GAAGTGCTGC CCGATCCTGC ACTTGAGCTT
    GAGAAGGAGG TGCCTCGTGA AAGGAGGAAG TCTAGCGACA GCATCTTGTC TACAAGGAGT
    ACCGACGATG GAGATGACTC GGGCGAGGAG AAGATATCCT CTGCGGTCTT CGTGCCCCAC
    CAAGCTCCAC AGGAACAGAT GGGAACTCCT GCAGACCCCG TGAGTATCCC GGGTCAAGCT
    AATGGGCAGA GGATATCGCC CAGAGCCGAC GGTTGGCTCG TCAAGGCTGA CGAGCCAGAA
    GTCGATCGTC GCGATGAAGC TGGTGATGAG TCAGGGGCAC TCACTTTGTC GGGGTCAGAC
    CGAACTTCTG TTACTCAAGG ATCCGGTCTA GAGCAGCCCG TCTCGAGGTT GCCCCCGCAA
    GGAGCCTCTG CTATCATAGA CTTCGCAGCA CCTAGAGGTG ATCTGAACGG GCCATCTCAG
    CAACTGCAAA CTGCAGTAAC CTATGAAGAT CAACCATCCC CGCAACCTTT GGAGGCGATT
    GAGTTGATTC CTTACAAACA CCAAGTCGGT GGTCACACAA CACTCTGGCG ATTCTCAAAG
    CGTGCAGTTT GCAAACAATT GAACAACCGG GAGAATGAGT TCTATGAGAG GATTGAGCGA
    GATGTTAGGG ATCTCCTCGC TTTCCTTCCT AGGTACATTG GGGTTCTCAA CGTGACTTTC
    AAAAAGCAGC CACGCCGCAA ATCCACGCTT AAGAGAGAGG ATACGGCTGC TTTAGAGCGA
    AAGCTGCTTG ACCAGAGCGA TTTGAAAAAG GCCCTAGGGA GCAATGGAGC TGTTGGTCAA
    CATGAAGCCG TGTCTAGCCC AGCTGAGGAT ACAAATGCGG CCCCTACGCA CACTAGAGTA
    ATCAGTCAAT CTCTGCAATC TAGTCAAATT CCGGTGCCTA CTGTCACTTT TGTTGATAAT
    CAACATATTC TGCCGCGCAA TCTGCTGCAG CCTTCGCCAA GTCCGACTAT CGAGGTCGGT
    AGCAGCCGCG TTAGAAGCAG CTCGGCTGTC ACCGTGCCAG GTTGTAGTCC GGATGGGCGG
    AAAAAGGCCG CGAGCCGGCC AACGCTTGAA GATCGACATG CGAACAGCTG GGGTGCCACA
    ACGGTCAACA AGGATTTGCG GAACAAGGTT TTCAACGACG TTTTCATGAA GACACCTTTT
    CCAATGCGGC CACACCGCAG GTGGCACTCG CAGCGTGCAC CCCTTCCAAG GTCCTCACTG
    CCGAACTTTT TGGTGCCTGC AGGCAGTGTT GAGGACAGGG CAGAATCCGA GCCTGATCTT
    CCGCCACGCC GCTCGAGCAC TGGTCAAGCC AGCTCTCGGT TACGCAACAG CTTGAGCGAT
    ATGGGGCCCG AAATTGATCA TTCAGAGAGC ACATCGGTGC CGAAGAACGT CCAGGACAGA
    CTCAAAGCAG AGGAAGATGT CGGACCTAAG GACATTACGG GCACAAGTGC ACCAGAGCCC
    GAGATTCCTG TCGAGAAGGG AGCTTCTCAG GGTAAACGTC GTCGGTATTC TGGTACGGCT
    TTGCGCAGGA GGGCCAAAGA GGGGTTGGAT TCATCTGAGA GGAAACTGCA ATACTTTCAA
    GAGCCCGACG ATGAGGACTT CAAACGGGAA ACTGATTTTG ATACCGGATT GGTTCCTGCA
    GCAGTGGAGG GAAACTCACA AAAAAACCCA ACACAAAACG ATAACGGGCG CCCCAGGAGC
    ACAGACTCAG ATGCAGGACC GGCGACTTTG CATCTTGAGC CAGAAGAGAC GCCTGTCACG
    TCCCCCGGCT GGACTATAAG CAATTACCCT TCAACAGTTA CATCGGGGGC CGCCAGCCCA
    TCGACCGAGT TCAAGCGAAT ATCGCGACCC GTCAACCCCA AGGAGGCCCG CGTGGCCGAT
    TCGCGCGTGG AATACTTCCT CCTGCTTGAG GACTTGACCG CAGGCATGAA ACGGCCATGT
    ATCATGGACC TCAAGATGGG GACCCGTCAA TACGGAGTCG ACGCGACCCC TAAGAAGCAG
    GCTTCACAGC AACGCAAGTG CGCAGCCACC ACGTCGCGGA GCCTCGGCGT ACGGGTCTGC
    GGGTTGCAAG TGTGGGACGC AGCGCAACAG ACATATATAT TTCGAGATAA GTACTATGGC
    CGGGACCTCA GACCTGGGGC CGAATTCCAG GAGGCGTTGA CGCGATTCCT GTACGACGGT
    GTGGATCCGG CGAGCATCCT TCGACACATA CCCACCGTCC TGTCCAAGCT TAGAGAGTTG
    GAGGTCATCA TTGAAAAGCT AGACGACTAC AGATTCTATG CGGCCAGCCT ATTGATGTTT
    TATGACGGAG ATCGGAGCGG CGATGACGGG AACGAGACCG CGATCGAGGA TTCGACTACG
    GACTTTGCCA CGGACACTGA AGAGGGACGC ACGGCTGCCT CGGTTGCCGC GCGCAAGAGT
    CGCAAGCGTA ACAAGCGCGA GATTGATTTC AAGATTGCCG ACTTTGCCAA CGGAGTCACA
    GCCTTGTCCG GTGGTTATGG GGCCGAGGAC AGGCCCTGTC CGCCACAGCA CCCCGGCCAG
    CCCGACCGCG GCTTTCTGAG AGGCCTAAGA AGTCTCCGGC GCTACTTTTT GAAGATCCAG
    AGGGAGACGA GGGAGCAGCT CGGACTCGGG CTTCTTGGCA GTCGTCACGG AGTCAATTAC
    GATGGCGCGG CCGACAGCGG CTTTGGTGGC GATGATGGAT GGGATGAGGA CGAGGGTTCT
    GTGAGCGAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of the rice blast fungus Magnaporthe grisea.
    Nature434(7036)980-6(2005 Apr)
    Dean RA,Talbot NJ,Ebbole DJ,Farman ML,Mitchell TK,Orbach MJ,Thon M,Kulkarni R,Xu JR,Pan H,Read ND,Lee YH,Carbone I,Brown D,Oh YY,Donofrio N,Jeong JS,Soanes DM,Djonovic S,Kolomiets E,Rehmeyer C,Li W,Harding M,Kim S,Lebrun MH,Bohnert H,Coughlan S,Butler J,Calvo S,Ma LJ,Nicol R,Purcell S,Nusbaum C,Galagan JE,Birren BW