GAPVD1 cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin)

Gene
Clones
gRNAs

Summary

Gene Symbol	GAPVD1
Entrez Gene ID	108494967
Full Name	GTPase activating protein and VPS9 domains 1
Gene Type	protein-coding
Organism	Lepidothrix coronata(blue-crowned manakin)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017810050.1	XP_017665539.1	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X3
XM_017810051.1	XP_017665540.1	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X4
XM_017810049.1	XP_017665538.1	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X2
XM_017810052.1	XP_017665541.1	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X5
XM_017810046.1	XP_017665535.1	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X1
XM_017810047.1	XP_017665536.1	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X1
XM_017810048.1	XP_017665537.1	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X1
XM_017810053.1	XP_017665542.1	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X6

Pathways from BioSystems
Gene Ontology

The following GAPVD1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GAPVD1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OLe54931	XM_017810046.1 Latest version!	Lepidothrix coronata GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OLe54931	XM_017810048.1 Latest version!	Lepidothrix coronata GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OLe54931	XM_017810047.1 Latest version!	Lepidothrix coronata GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OLe54932	XM_017810049.1 Latest version!	Lepidothrix coronata GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OLe54933	XM_017810050.1 Latest version!	Lepidothrix coronata GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OLe54934	XM_017810051.1 Latest version!	Lepidothrix coronata GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OLe54935	XM_017810052.1 Latest version!	Lepidothrix coronata GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OLe54936	XM_017810053.1 Latest version!	Lepidothrix coronata GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GAPVD1 ( XM_017810046.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017665535.1 Lepidothrix coronata GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OLe54931
Clone ID Related Accession (Same CDS sequence)	XM_017810046.1 , XM_017810047.1 , XM_017810048.1
Accession Version	XM_017810046.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690208.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGC TGGACATCCA CACGCTGGCC CACCACCTGA AGCAGGAGCG GCTCTACGTG 
ACGGCGGAGA AGCAGCTGAT CCAGCGGCTC AACGCCGACG TGCTGAAGAC GGCGGAGAAG 
CTCTACCGGA CAGCCTGGAT CTCCAAACAG CAGCGCATCA ACCTGGACAG GCTCATCATC 
ACCAGTGCTG AAGCTTCTCC TGCTGAATGT TGCCAACATG CAAAAATCTT GGAGGACACA 
CAGTTTGTGG ATGGATATAA GCAGCTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCCTG 
AACAGACTGA GAGAGAACCC GAGGCTTATT GCATCCTGCC TGGTTGCTGG AGAGAAGCTC 
AACCAGGACA ACACTCAGAG TGTCATTCAC ACAGTCTTTA CCTCCATTTA TGGCAACTGC 
ATCATGCAGG AGGATGAGAG TTACCTGCTC CAGGTCCTCC GTTACCTGAT TGAGTTTGAA 
CTCAAGGAGA GCGACAACCC CCGGCGGCTG CTCCGACGAG GCACCTGTGC CTTCAGCATC 
TTGTTCAAAC TCTTCTCTGA AGGACTCTTC TCTGCAAAAC TTTTTCTTAC TGCAACCTTA 
CATGAGCCAA TCATGCAGCT CCTGGTTGAA GATGAAGACC ACCTGGAAAC CGATGCAAAC 
AAGTTAATTG AGAGATTCTC CCCAGTACAG CAGGAAAAGT TATTTGGAGA GAAAGGCACA 
GAAAAGTTCA AGCAAAGAGT CCAAGAGATG GTTGACTCCA ACGAGGCCAA GCTGGTGACT 
TTGGTCAACA AATTCATTGG CTATCTCAAA CAAAACACTT ACTGTTTTCC TCACAGCTTG 
AGGTGGATTG TGTCGCAGAT GTACAAAACG CTGTCGTGTG TGGACAGGCT GGAGGTTGGG 
GAGGTCAGAG CCATGTGCAC AGATCTTCTT CTGGCCTGTT TCATCTGCCC TGCAATCGTT 
AACCCAGAGC AATATGGGAT CATTTCTGAT GCTCCTATAA ATGAGGTGGC AAGATTTAAT 
CTGATGCAGG TTGGGAGGCT CCTGCAGCAG CTGGCAATGA CAGGCTCTGA GGAGGGAGAT 
CCACGTACCA AGAGCAGCCT TGCTAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTGGAT 
GTGGTCATCG ACGGGCGCGC GGTGGAGACG CCTCCGATGT CTGCTGTCAA CCTGCTGGAG 
GGGCTGAGCA GGACCGTGGT GTACATGACA TACAGCCAGC TGACCACTCT GGTTGGCTTT 
ATGCGCAACG TGATGTCAAG CGATCAGCTT AAGGAAGATC GGATGGCTTT GGAAAACTTG 
CTGGCAAACT TACCCCAGAA CAAGCCAGGG AAAAGCAGCA GCCTTGAAAT GACTCCATAT 
AACACCCCCC AGCTTTCTCC TGCGACCACT CCAGCCAACA AAAAAAACCG GTTACCGATA 
GGACAGCAAT TGGCAGCCAT TACTGCCTGG GACACCTCTG CCACCAATCT GTCAGCTCAC 
ATAACTCTAG TAACCCCTTT TGCAACTCGT AGCAGAAGTA GATCAAATAT TCTGATGGAT 
CAGCACGGAG ATCACGAAGG ATCCTCCCAG GAAACTATCC CAGAAGTTCA GCCAGAAGAA 
GTGCTGGTGA TTTCTCTGGG GACAGGTCCA CAGATTACTC CAGGAATGAT GTCAGAAAAT 
GAGGTCTTAA ACATGCAGCT TGCAGATGGC GGGCAAGATG TCCCCATTGA CGAAAACAAA 
CTCCATGGTA AACCTGATAA AACCTTGCGC TTTTCCCTCT GCAGTGATAA TCTGGAAGGA 
ATATCTGAAG GTCCTTCCAA TCGCTCCAAC TCCGTGTCCT CGCTGGATTT GGAAGGGGAG 
TCGGTGTCGG AGCTCGGCGC GGGCCCCTCC GGGAGCAACG GTGTGGAGGC TCTGCAGCTC 
CTGGAGCATG AACAAGCCAC AACTCAGGAT AATCTGGATG ACAAGCTCCG TAAGTTTGAA 
ATCCGTGATA TGATGGGTTT GACTGATGAC AGAGATATAT CAGAAACTGT GAGTGAAACC 
TGGAGCACAG ATGTCTTGGG AAGTGACTTC GACCCCAACA TGGACGAGGA CCGGCTGCAG 
GAGATCGCAG GTGCAGCTGC AGAGAACATG CTAGGCAGCT TGCTGTGTTT GCCAGGTTCA 
GGATCCGTGT TGCTTGATCC CTGCACAGGT TCAACCATAT CAGAAACCAC AAGTGAGGCG 
TGGAGTGTGG AAGTATTACC GAGTGATTCA GAGGCTCCAG ACTTAAAACA GGAGGAAAGG 
CTCCAAGAAC TGGAGAGCTG TTCTGGGCTG GGCAGCACGT CTGATGACAC AGATGTGAGG 
GAGGTCAGCT CTCGACCCAG CACTCCAGGA CTCAGCGTTG TGTCAGGTAT TAGTGCAACA 
TCTGAAGATA TTCCTAACAA GATTGAGGAT CTCAGATCTG AATGTAGCTC TGACTTTGGG 
GGAAAAGATT CTGTGACAAG TCCTGATATG GATGAAACAG CCCATGGAGC CAGTCAATTG 
ACATCTCCAC CTTCTCAGAC AGATTCTTTG CTTGCACTGT TTGACCCTCT GTCCTCAAAT 
GAGGGTGTGT CAGCTGTAGT AAGGCCTAAA GTGCACTATG CAAGACCCTC TCACCCACCT 
CCGGATCCAC CCATCCTGGA AGGAGCTGTG GGAGGTAACG AGGCCCGACT GCCAAACTTT 
GGGTCTCACG CTCTGATTCC AACCGATCTG GAAGCCTTCA AGCAGAGACA TTCCTACCCG 
GAGAGGCTGG TCCGCAGCCG GAGCTCAGAC ATAGTGTCGT CGGTTCGGAG GCCCATGAGT 
GATCCCGGGT GGAACAGGCG TCCTGGGAAC GAGGACAGGG AGCTGCCCAT GGCCACCAGC 
AGCGCTGGAG CAGCTGTGCT GGCAGCCACG TCTCAGTCAT CGTCTTCATC TCCCAGTAAG 
GACTCCTCCA GGGGAGAGAT TGAGGAACGA AAAGACAGCG ATGATGAGAA GTCTGACAGG 
AACAAGCCCT GGTGGAGGAA ACGTTTTGCG TCTGCCATGC CCAAAGCTCC TATTCCATTT 
AGAAAGAAAG AAAAACAAGA GAAAGACAAA GATGACATGG TGCCTGACAG ATACGCAACA 
CTTCAAGATG ATCCCAGCCC AAGGCTCAGT GCACAGGCAC AAGCTGCTGA GGACATTCTG 
GACAAATATA GGAATGCCAT CAAGAGAACC AGTCCCAGTG AGGGAGCCAT AGTGAACTAT 
GACAGTGCAG AGGCTCTTGG GGATGGTGAG AGCATGCACG ACTCCCCGCG GGACGAGGCC 
TTGCAGAACA TGTCTGCAGA CGATCTCCCA GACTCTGCAA GTCAAGTAGC ACAGCCACAA 
AGTTCTGCTT TCTCCTATAG GGATGCAAAG AAGAAATTGA GATTGGCTCT TTGTTCAGCA 
GATTCTGTTG CCTTCCCGAT GTTGACCCAC TCAACACGGA ATGGTCTCCC AGACCACACA 
GACCCCGAAG ATAATGAAAT TGTGTGCTTC TTGAAAGTTC AGCTGGCTGA AGCCATCAAC 
CTCCAGGACA AGAACCTGAT GGCACAGCTG CAGGAGACGA TGCGCTGCGT GAGCCGCTTC 
GACAACAGGA CCTGTCGGAA GCTGCTGGCC TCCATTGCAG AGGACTATAG GAAAAGAGCT 
CCATATATTG CTTATTTGAC TCGCTGTCGC CAAGGCCTGC AGACGACACA GGCGCACCTG 
GAAAGGCTCT TGCAGAGAGT TCTACGAGAT AAAGAAGTGG CCAACAGATA CTTCACTACA 
GTATGTGTGA GGTTACTGCT GGAGAGCAAA GAAAAGAAAA TAAGGGAATT TATTCAAGAT 
TTCCAGAAGC TCACAGCAGC AGACGATAAA ACAGCCCAAG TTGAGGATTT CCTTCAGTTC 
CTGTACGGGG CTATGGCTCA GGATGCCATA TGGCAGAATG CCAGTGAGGA ACAGCTTCAG 
GATGCACAAT TAGCCATCGA GCGCAGTGTG ATGAATCGCA TTTTCAAACT TGCCTTCTAC 
CCTAATCAGG ATGGAGATAT TCTGCGTGAC CAGGTCCTTC ACGAGCACAT ACAGAGGTTA 
TCCAAAGTAG TGACTGCAAA CCACAAGGCC CTTCAGATAC CTGAGGTGTA TCTCCGGGAG 
GCACCGTGGC CGTCGGCACA GTCCGAGATC CGCACGATAA GTGCTTACAA AACCCCCCGG 
GACAAGGTGC AGTGTATCCT GAGGATGTGC TCCACCATCA TGAACCTGCT CAGCCTGGCC 
AACGAGGATT CAGTACCTGG GGCAGATGAT TTTGTTCCTG TTTTGGTTTT TGTCCTGATA 
AAGGCAAACC CCCCTTGCCT GCTGTCCACT GTGCAGTACA TCAGTAGTTT CTATGCCAAC 
TGCTTGTCTG GAGAGGAGTC GTACTGGTGG ATGCAGTTCA CGGCAGCCGT GGAGTTCATC 
AAAACCATCG ATGATCGCAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017665535.1
CDS	162..4625
Translation	MVKLDIHTLAHHLKQERLYVTAEKQLIQRLNADVLKTAEKLYRTAWISKQQRINLDRLIITSAEASPAECCQHAKILEDTQFVDGYKQLGFQETAYGEFLNRLRENPRLIASCLVAGEKLNQDNTQSVIHTVFTSIYGNCIMQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDANKLIERFSPVQQEKLFGEKGTEKFKQRVQEMVDSNEAKLVTLVNKFIGYLKQNTYCFPHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAIVNPEQYGIISDAPINEVARFNLMQVGRLLQQLAMTGSEEGDPRTKSSLAKFDKSCVAAFLDVVIDGRAVETPPMSAVNLLEGLSRTVVYMTYSQLTTLVGFMRNVMSSDQLKEDRMALENLLANLPQNKPGKSSSLEMTPYNTPQLSPATTPANKKNRLPIGQQLAAITAWDTSATNLSAHITLVTPFATRSRSRSNILMDQHGDHEGSSQETIPEVQPEEVLVISLGTGPQITPGMMSENEVLNMQLADGGQDVPIDENKLHGKPDKTLRFSLCSDNLEGISEGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNMDEDRLQEIAGAAAENMLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDETAHGASQLTSPPSQTDSLLALFDPLSSNEGVSAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHALIPTDLEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPGWNRRPGNEDRELPMATSSAGAAVLAATSQSSSSSPSKDSSRGEIEERKDSDDEKSDRNKPWWRKRFASAMPKAPIPFRKKEKQEKDKDDMVPDRYATLQDDPSPRLSAQAQAAEDILDKYRNAIKRTSPSEGAIVNYDSAEALGDGESMHDSPRDEALQNMSADDLPDSASQVAQPQSSAFSYRDAKKKLRLALCSADSVAFPMLTHSTRNGLPDHTDPEDNEIVCFLKVQLAEAINLQDKNLMAQLQETMRCVSRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGAMAQDAIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHKALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYANCLSGEESYWWMQFTAAVEFIKTIDDRK

Target ORF information:

RefSeq Version	XM_017810046.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017810046.1

ORF Insert Sequence:

ATGGTGAAGC TGGACATCCA CACGCTGGCC CACCACCTGA AGCAGGAGCG GCTCTACGTG 
ACGGCGGAGA AGCAGCTGAT CCAGCGGCTC AACGCCGACG TGCTGAAGAC GGCGGAGAAG 
CTCTACCGGA CAGCCTGGAT CTCCAAACAG CAGCGCATCA ACCTGGACAG GCTCATCATC 
ACCAGTGCTG AAGCTTCTCC TGCTGAATGT TGCCAACATG CAAAAATCTT GGAGGACACA 
CAGTTTGTGG ATGGATATAA GCAGCTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCCTG 
AACAGACTGA GAGAGAACCC GAGGCTTATT GCATCCTGCC TGGTTGCTGG AGAGAAGCTC 
AACCAGGACA ACACTCAGAG TGTCATTCAC ACAGTCTTTA CCTCCATTTA TGGCAACTGC 
ATCATGCAGG AGGATGAGAG TTACCTGCTC CAGGTCCTCC GTTACCTGAT TGAGTTTGAA 
CTCAAGGAGA GCGACAACCC CCGGCGGCTG CTCCGACGAG GCACCTGTGC CTTCAGCATC 
TTGTTCAAAC TCTTCTCTGA AGGACTCTTC TCTGCAAAAC TTTTTCTTAC TGCAACCTTA 
CATGAGCCAA TCATGCAGCT CCTGGTTGAA GATGAAGACC ACCTGGAAAC CGATGCAAAC 
AAGTTAATTG AGAGATTCTC CCCAGTACAG CAGGAAAAGT TATTTGGAGA GAAAGGCACA 
GAAAAGTTCA AGCAAAGAGT CCAAGAGATG GTTGACTCCA ACGAGGCCAA GCTGGTGACT 
TTGGTCAACA AATTCATTGG CTATCTCAAA CAAAACACTT ACTGTTTTCC TCACAGCTTG 
AGGTGGATTG TGTCGCAGAT GTACAAAACG CTGTCGTGTG TGGACAGGCT GGAGGTTGGG 
GAGGTCAGAG CCATGTGCAC AGATCTTCTT CTGGCCTGTT TCATCTGCCC TGCAATCGTT 
AACCCAGAGC AATATGGGAT CATTTCTGAT GCTCCTATAA ATGAGGTGGC AAGATTTAAT 
CTGATGCAGG TTGGGAGGCT CCTGCAGCAG CTGGCAATGA CAGGCTCTGA GGAGGGAGAT 
CCACGTACCA AGAGCAGCCT TGCTAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTGGAT 
GTGGTCATCG ACGGGCGCGC GGTGGAGACG CCTCCGATGT CTGCTGTCAA CCTGCTGGAG 
GGGCTGAGCA GGACCGTGGT GTACATGACA TACAGCCAGC TGACCACTCT GGTTGGCTTT 
ATGCGCAACG TGATGTCAAG CGATCAGCTT AAGGAAGATC GGATGGCTTT GGAAAACTTG 
CTGGCAAACT TACCCCAGAA CAAGCCAGGG AAAAGCAGCA GCCTTGAAAT GACTCCATAT 
AACACCCCCC AGCTTTCTCC TGCGACCACT CCAGCCAACA AAAAAAACCG GTTACCGATA 
GGACAGCAAT TGGCAGCCAT TACTGCCTGG GACACCTCTG CCACCAATCT GTCAGCTCAC 
ATAACTCTAG TAACCCCTTT TGCAACTCGT AGCAGAAGTA GATCAAATAT TCTGATGGAT 
CAGCACGGAG ATCACGAAGG ATCCTCCCAG GAAACTATCC CAGAAGTTCA GCCAGAAGAA 
GTGCTGGTGA TTTCTCTGGG GACAGGTCCA CAGATTACTC CAGGAATGAT GTCAGAAAAT 
GAGGTCTTAA ACATGCAGCT TGCAGATGGC GGGCAAGATG TCCCCATTGA CGAAAACAAA 
CTCCATGGTA AACCTGATAA AACCTTGCGC TTTTCCCTCT GCAGTGATAA TCTGGAAGGA 
ATATCTGAAG GTCCTTCCAA TCGCTCCAAC TCCGTGTCCT CGCTGGATTT GGAAGGGGAG 
TCGGTGTCGG AGCTCGGCGC GGGCCCCTCC GGGAGCAACG GTGTGGAGGC TCTGCAGCTC 
CTGGAGCATG AACAAGCCAC AACTCAGGAT AATCTGGATG ACAAGCTCCG TAAGTTTGAA 
ATCCGTGATA TGATGGGTTT GACTGATGAC AGAGATATAT CAGAAACTGT GAGTGAAACC 
TGGAGCACAG ATGTCTTGGG AAGTGACTTC GACCCCAACA TGGACGAGGA CCGGCTGCAG 
GAGATCGCAG GTGCAGCTGC AGAGAACATG CTAGGCAGCT TGCTGTGTTT GCCAGGTTCA 
GGATCCGTGT TGCTTGATCC CTGCACAGGT TCAACCATAT CAGAAACCAC AAGTGAGGCG 
TGGAGTGTGG AAGTATTACC GAGTGATTCA GAGGCTCCAG ACTTAAAACA GGAGGAAAGG 
CTCCAAGAAC TGGAGAGCTG TTCTGGGCTG GGCAGCACGT CTGATGACAC AGATGTGAGG 
GAGGTCAGCT CTCGACCCAG CACTCCAGGA CTCAGCGTTG TGTCAGGTAT TAGTGCAACA 
TCTGAAGATA TTCCTAACAA GATTGAGGAT CTCAGATCTG AATGTAGCTC TGACTTTGGG 
GGAAAAGATT CTGTGACAAG TCCTGATATG GATGAAACAG CCCATGGAGC CAGTCAATTG 
ACATCTCCAC CTTCTCAGAC AGATTCTTTG CTTGCACTGT TTGACCCTCT GTCCTCAAAT 
GAGGGTGTGT CAGCTGTAGT AAGGCCTAAA GTGCACTATG CAAGACCCTC TCACCCACCT 
CCGGATCCAC CCATCCTGGA AGGAGCTGTG GGAGGTAACG AGGCCCGACT GCCAAACTTT 
GGGTCTCACG CTCTGATTCC AACCGATCTG GAAGCCTTCA AGCAGAGACA TTCCTACCCG 
GAGAGGCTGG TCCGCAGCCG GAGCTCAGAC ATAGTGTCGT CGGTTCGGAG GCCCATGAGT 
GATCCCGGGT GGAACAGGCG TCCTGGGAAC GAGGACAGGG AGCTGCCCAT GGCCACCAGC 
AGCGCTGGAG CAGCTGTGCT GGCAGCCACG TCTCAGTCAT CGTCTTCATC TCCCAGTAAG 
GACTCCTCCA GGGGAGAGAT TGAGGAACGA AAAGACAGCG ATGATGAGAA GTCTGACAGG 
AACAAGCCCT GGTGGAGGAA ACGTTTTGCG TCTGCCATGC CCAAAGCTCC TATTCCATTT 
AGAAAGAAAG AAAAACAAGA GAAAGACAAA GATGACATGG TGCCTGACAG ATACGCAACA 
CTTCAAGATG ATCCCAGCCC AAGGCTCAGT GCACAGGCAC AAGCTGCTGA GGACATTCTG 
GACAAATATA GGAATGCCAT CAAGAGAACC AGTCCCAGTG AGGGAGCCAT AGTGAACTAT 
GACAGTGCAG AGGCTCTTGG GGATGGTGAG AGCATGCACG ACTCCCCGCG GGACGAGGCC 
TTGCAGAACA TGTCTGCAGA CGATCTCCCA GACTCTGCAA GTCAAGTAGC ACAGCCACAA 
AGTTCTGCTT TCTCCTATAG GGATGCAAAG AAGAAATTGA GATTGGCTCT TTGTTCAGCA 
GATTCTGTTG CCTTCCCGAT GTTGACCCAC TCAACACGGA ATGGTCTCCC AGACCACACA 
GACCCCGAAG ATAATGAAAT TGTGTGCTTC TTGAAAGTTC AGCTGGCTGA AGCCATCAAC 
CTCCAGGACA AGAACCTGAT GGCACAGCTG CAGGAGACGA TGCGCTGCGT GAGCCGCTTC 
GACAACAGGA CCTGTCGGAA GCTGCTGGCC TCCATTGCAG AGGACTATAG GAAAAGAGCT 
CCATATATTG CTTATTTGAC TCGCTGTCGC CAAGGCCTGC AGACGACACA GGCGCACCTG 
GAAAGGCTCT TGCAGAGAGT TCTACGAGAT AAAGAAGTGG CCAACAGATA CTTCACTACA 
GTATGTGTGA GGTTACTGCT GGAGAGCAAA GAAAAGAAAA TAAGGGAATT TATTCAAGAT 
TTCCAGAAGC TCACAGCAGC AGACGATAAA ACAGCCCAAG TTGAGGATTT CCTTCAGTTC 
CTGTACGGGG CTATGGCTCA GGATGCCATA TGGCAGAATG CCAGTGAGGA ACAGCTTCAG 
GATGCACAAT TAGCCATCGA GCGCAGTGTG ATGAATCGCA TTTTCAAACT TGCCTTCTAC 
CCTAATCAGG ATGGAGATAT TCTGCGTGAC CAGGTCCTTC ACGAGCACAT ACAGAGGTTA 
TCCAAAGTAG TGACTGCAAA CCACAAGGCC CTTCAGATAC CTGAGGTGTA TCTCCGGGAG 
GCACCGTGGC CGTCGGCACA GTCCGAGATC CGCACGATAA GTGCTTACAA AACCCCCCGG 
GACAAGGTGC AGTGTATCCT GAGGATGTGC TCCACCATCA TGAACCTGCT CAGCCTGGCC 
AACGAGGATT CAGTACCTGG GGCAGATGAT TTTGTTCCTG TTTTGGTTTT TGTCCTGATA 
AAGGCAAACC CCCCTTGCCT GCTGTCCACT GTGCAGTACA TCAGTAGTTT CTATGCCAAC 
TGCTTGTCTG GAGAGGAGTC GTACTGGTGG ATGCAGTTCA CGGCAGCCGT GGAGTTCATC 
AAAACCATCG ATGATCGCAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPVD1 ( XM_017810047.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017665536.1 Lepidothrix coronata GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OLe54931
Clone ID Related Accession (Same CDS sequence)	XM_017810046.1 , XM_017810047.1 , XM_017810048.1
Accession Version	XM_017810047.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690208.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGC TGGACATCCA CACGCTGGCC CACCACCTGA AGCAGGAGCG GCTCTACGTG 
ACGGCGGAGA AGCAGCTGAT CCAGCGGCTC AACGCCGACG TGCTGAAGAC GGCGGAGAAG 
CTCTACCGGA CAGCCTGGAT CTCCAAACAG CAGCGCATCA ACCTGGACAG GCTCATCATC 
ACCAGTGCTG AAGCTTCTCC TGCTGAATGT TGCCAACATG CAAAAATCTT GGAGGACACA 
CAGTTTGTGG ATGGATATAA GCAGCTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCCTG 
AACAGACTGA GAGAGAACCC GAGGCTTATT GCATCCTGCC TGGTTGCTGG AGAGAAGCTC 
AACCAGGACA ACACTCAGAG TGTCATTCAC ACAGTCTTTA CCTCCATTTA TGGCAACTGC 
ATCATGCAGG AGGATGAGAG TTACCTGCTC CAGGTCCTCC GTTACCTGAT TGAGTTTGAA 
CTCAAGGAGA GCGACAACCC CCGGCGGCTG CTCCGACGAG GCACCTGTGC CTTCAGCATC 
TTGTTCAAAC TCTTCTCTGA AGGACTCTTC TCTGCAAAAC TTTTTCTTAC TGCAACCTTA 
CATGAGCCAA TCATGCAGCT CCTGGTTGAA GATGAAGACC ACCTGGAAAC CGATGCAAAC 
AAGTTAATTG AGAGATTCTC CCCAGTACAG CAGGAAAAGT TATTTGGAGA GAAAGGCACA 
GAAAAGTTCA AGCAAAGAGT CCAAGAGATG GTTGACTCCA ACGAGGCCAA GCTGGTGACT 
TTGGTCAACA AATTCATTGG CTATCTCAAA CAAAACACTT ACTGTTTTCC TCACAGCTTG 
AGGTGGATTG TGTCGCAGAT GTACAAAACG CTGTCGTGTG TGGACAGGCT GGAGGTTGGG 
GAGGTCAGAG CCATGTGCAC AGATCTTCTT CTGGCCTGTT TCATCTGCCC TGCAATCGTT 
AACCCAGAGC AATATGGGAT CATTTCTGAT GCTCCTATAA ATGAGGTGGC AAGATTTAAT 
CTGATGCAGG TTGGGAGGCT CCTGCAGCAG CTGGCAATGA CAGGCTCTGA GGAGGGAGAT 
CCACGTACCA AGAGCAGCCT TGCTAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTGGAT 
GTGGTCATCG ACGGGCGCGC GGTGGAGACG CCTCCGATGT CTGCTGTCAA CCTGCTGGAG 
GGGCTGAGCA GGACCGTGGT GTACATGACA TACAGCCAGC TGACCACTCT GGTTGGCTTT 
ATGCGCAACG TGATGTCAAG CGATCAGCTT AAGGAAGATC GGATGGCTTT GGAAAACTTG 
CTGGCAAACT TACCCCAGAA CAAGCCAGGG AAAAGCAGCA GCCTTGAAAT GACTCCATAT 
AACACCCCCC AGCTTTCTCC TGCGACCACT CCAGCCAACA AAAAAAACCG GTTACCGATA 
GGACAGCAAT TGGCAGCCAT TACTGCCTGG GACACCTCTG CCACCAATCT GTCAGCTCAC 
ATAACTCTAG TAACCCCTTT TGCAACTCGT AGCAGAAGTA GATCAAATAT TCTGATGGAT 
CAGCACGGAG ATCACGAAGG ATCCTCCCAG GAAACTATCC CAGAAGTTCA GCCAGAAGAA 
GTGCTGGTGA TTTCTCTGGG GACAGGTCCA CAGATTACTC CAGGAATGAT GTCAGAAAAT 
GAGGTCTTAA ACATGCAGCT TGCAGATGGC GGGCAAGATG TCCCCATTGA CGAAAACAAA 
CTCCATGGTA AACCTGATAA AACCTTGCGC TTTTCCCTCT GCAGTGATAA TCTGGAAGGA 
ATATCTGAAG GTCCTTCCAA TCGCTCCAAC TCCGTGTCCT CGCTGGATTT GGAAGGGGAG 
TCGGTGTCGG AGCTCGGCGC GGGCCCCTCC GGGAGCAACG GTGTGGAGGC TCTGCAGCTC 
CTGGAGCATG AACAAGCCAC AACTCAGGAT AATCTGGATG ACAAGCTCCG TAAGTTTGAA 
ATCCGTGATA TGATGGGTTT GACTGATGAC AGAGATATAT CAGAAACTGT GAGTGAAACC 
TGGAGCACAG ATGTCTTGGG AAGTGACTTC GACCCCAACA TGGACGAGGA CCGGCTGCAG 
GAGATCGCAG GTGCAGCTGC AGAGAACATG CTAGGCAGCT TGCTGTGTTT GCCAGGTTCA 
GGATCCGTGT TGCTTGATCC CTGCACAGGT TCAACCATAT CAGAAACCAC AAGTGAGGCG 
TGGAGTGTGG AAGTATTACC GAGTGATTCA GAGGCTCCAG ACTTAAAACA GGAGGAAAGG 
CTCCAAGAAC TGGAGAGCTG TTCTGGGCTG GGCAGCACGT CTGATGACAC AGATGTGAGG 
GAGGTCAGCT CTCGACCCAG CACTCCAGGA CTCAGCGTTG TGTCAGGTAT TAGTGCAACA 
TCTGAAGATA TTCCTAACAA GATTGAGGAT CTCAGATCTG AATGTAGCTC TGACTTTGGG 
GGAAAAGATT CTGTGACAAG TCCTGATATG GATGAAACAG CCCATGGAGC CAGTCAATTG 
ACATCTCCAC CTTCTCAGAC AGATTCTTTG CTTGCACTGT TTGACCCTCT GTCCTCAAAT 
GAGGGTGTGT CAGCTGTAGT AAGGCCTAAA GTGCACTATG CAAGACCCTC TCACCCACCT 
CCGGATCCAC CCATCCTGGA AGGAGCTGTG GGAGGTAACG AGGCCCGACT GCCAAACTTT 
GGGTCTCACG CTCTGATTCC AACCGATCTG GAAGCCTTCA AGCAGAGACA TTCCTACCCG 
GAGAGGCTGG TCCGCAGCCG GAGCTCAGAC ATAGTGTCGT CGGTTCGGAG GCCCATGAGT 
GATCCCGGGT GGAACAGGCG TCCTGGGAAC GAGGACAGGG AGCTGCCCAT GGCCACCAGC 
AGCGCTGGAG CAGCTGTGCT GGCAGCCACG TCTCAGTCAT CGTCTTCATC TCCCAGTAAG 
GACTCCTCCA GGGGAGAGAT TGAGGAACGA AAAGACAGCG ATGATGAGAA GTCTGACAGG 
AACAAGCCCT GGTGGAGGAA ACGTTTTGCG TCTGCCATGC CCAAAGCTCC TATTCCATTT 
AGAAAGAAAG AAAAACAAGA GAAAGACAAA GATGACATGG TGCCTGACAG ATACGCAACA 
CTTCAAGATG ATCCCAGCCC AAGGCTCAGT GCACAGGCAC AAGCTGCTGA GGACATTCTG 
GACAAATATA GGAATGCCAT CAAGAGAACC AGTCCCAGTG AGGGAGCCAT AGTGAACTAT 
GACAGTGCAG AGGCTCTTGG GGATGGTGAG AGCATGCACG ACTCCCCGCG GGACGAGGCC 
TTGCAGAACA TGTCTGCAGA CGATCTCCCA GACTCTGCAA GTCAAGTAGC ACAGCCACAA 
AGTTCTGCTT TCTCCTATAG GGATGCAAAG AAGAAATTGA GATTGGCTCT TTGTTCAGCA 
GATTCTGTTG CCTTCCCGAT GTTGACCCAC TCAACACGGA ATGGTCTCCC AGACCACACA 
GACCCCGAAG ATAATGAAAT TGTGTGCTTC TTGAAAGTTC AGCTGGCTGA AGCCATCAAC 
CTCCAGGACA AGAACCTGAT GGCACAGCTG CAGGAGACGA TGCGCTGCGT GAGCCGCTTC 
GACAACAGGA CCTGTCGGAA GCTGCTGGCC TCCATTGCAG AGGACTATAG GAAAAGAGCT 
CCATATATTG CTTATTTGAC TCGCTGTCGC CAAGGCCTGC AGACGACACA GGCGCACCTG 
GAAAGGCTCT TGCAGAGAGT TCTACGAGAT AAAGAAGTGG CCAACAGATA CTTCACTACA 
GTATGTGTGA GGTTACTGCT GGAGAGCAAA GAAAAGAAAA TAAGGGAATT TATTCAAGAT 
TTCCAGAAGC TCACAGCAGC AGACGATAAA ACAGCCCAAG TTGAGGATTT CCTTCAGTTC 
CTGTACGGGG CTATGGCTCA GGATGCCATA TGGCAGAATG CCAGTGAGGA ACAGCTTCAG 
GATGCACAAT TAGCCATCGA GCGCAGTGTG ATGAATCGCA TTTTCAAACT TGCCTTCTAC 
CCTAATCAGG ATGGAGATAT TCTGCGTGAC CAGGTCCTTC ACGAGCACAT ACAGAGGTTA 
TCCAAAGTAG TGACTGCAAA CCACAAGGCC CTTCAGATAC CTGAGGTGTA TCTCCGGGAG 
GCACCGTGGC CGTCGGCACA GTCCGAGATC CGCACGATAA GTGCTTACAA AACCCCCCGG 
GACAAGGTGC AGTGTATCCT GAGGATGTGC TCCACCATCA TGAACCTGCT CAGCCTGGCC 
AACGAGGATT CAGTACCTGG GGCAGATGAT TTTGTTCCTG TTTTGGTTTT TGTCCTGATA 
AAGGCAAACC CCCCTTGCCT GCTGTCCACT GTGCAGTACA TCAGTAGTTT CTATGCCAAC 
TGCTTGTCTG GAGAGGAGTC GTACTGGTGG ATGCAGTTCA CGGCAGCCGT GGAGTTCATC 
AAAACCATCG ATGATCGCAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017665536.1
CDS	154..4617
Translation	MVKLDIHTLAHHLKQERLYVTAEKQLIQRLNADVLKTAEKLYRTAWISKQQRINLDRLIITSAEASPAECCQHAKILEDTQFVDGYKQLGFQETAYGEFLNRLRENPRLIASCLVAGEKLNQDNTQSVIHTVFTSIYGNCIMQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDANKLIERFSPVQQEKLFGEKGTEKFKQRVQEMVDSNEAKLVTLVNKFIGYLKQNTYCFPHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAIVNPEQYGIISDAPINEVARFNLMQVGRLLQQLAMTGSEEGDPRTKSSLAKFDKSCVAAFLDVVIDGRAVETPPMSAVNLLEGLSRTVVYMTYSQLTTLVGFMRNVMSSDQLKEDRMALENLLANLPQNKPGKSSSLEMTPYNTPQLSPATTPANKKNRLPIGQQLAAITAWDTSATNLSAHITLVTPFATRSRSRSNILMDQHGDHEGSSQETIPEVQPEEVLVISLGTGPQITPGMMSENEVLNMQLADGGQDVPIDENKLHGKPDKTLRFSLCSDNLEGISEGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNMDEDRLQEIAGAAAENMLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDETAHGASQLTSPPSQTDSLLALFDPLSSNEGVSAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHALIPTDLEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPGWNRRPGNEDRELPMATSSAGAAVLAATSQSSSSSPSKDSSRGEIEERKDSDDEKSDRNKPWWRKRFASAMPKAPIPFRKKEKQEKDKDDMVPDRYATLQDDPSPRLSAQAQAAEDILDKYRNAIKRTSPSEGAIVNYDSAEALGDGESMHDSPRDEALQNMSADDLPDSASQVAQPQSSAFSYRDAKKKLRLALCSADSVAFPMLTHSTRNGLPDHTDPEDNEIVCFLKVQLAEAINLQDKNLMAQLQETMRCVSRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGAMAQDAIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHKALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYANCLSGEESYWWMQFTAAVEFIKTIDDRK

Target ORF information:

RefSeq Version	XM_017810047.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017810047.1

ORF Insert Sequence:

ATGGTGAAGC TGGACATCCA CACGCTGGCC CACCACCTGA AGCAGGAGCG GCTCTACGTG 
ACGGCGGAGA AGCAGCTGAT CCAGCGGCTC AACGCCGACG TGCTGAAGAC GGCGGAGAAG 
CTCTACCGGA CAGCCTGGAT CTCCAAACAG CAGCGCATCA ACCTGGACAG GCTCATCATC 
ACCAGTGCTG AAGCTTCTCC TGCTGAATGT TGCCAACATG CAAAAATCTT GGAGGACACA 
CAGTTTGTGG ATGGATATAA GCAGCTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCCTG 
AACAGACTGA GAGAGAACCC GAGGCTTATT GCATCCTGCC TGGTTGCTGG AGAGAAGCTC 
AACCAGGACA ACACTCAGAG TGTCATTCAC ACAGTCTTTA CCTCCATTTA TGGCAACTGC 
ATCATGCAGG AGGATGAGAG TTACCTGCTC CAGGTCCTCC GTTACCTGAT TGAGTTTGAA 
CTCAAGGAGA GCGACAACCC CCGGCGGCTG CTCCGACGAG GCACCTGTGC CTTCAGCATC 
TTGTTCAAAC TCTTCTCTGA AGGACTCTTC TCTGCAAAAC TTTTTCTTAC TGCAACCTTA 
CATGAGCCAA TCATGCAGCT CCTGGTTGAA GATGAAGACC ACCTGGAAAC CGATGCAAAC 
AAGTTAATTG AGAGATTCTC CCCAGTACAG CAGGAAAAGT TATTTGGAGA GAAAGGCACA 
GAAAAGTTCA AGCAAAGAGT CCAAGAGATG GTTGACTCCA ACGAGGCCAA GCTGGTGACT 
TTGGTCAACA AATTCATTGG CTATCTCAAA CAAAACACTT ACTGTTTTCC TCACAGCTTG 
AGGTGGATTG TGTCGCAGAT GTACAAAACG CTGTCGTGTG TGGACAGGCT GGAGGTTGGG 
GAGGTCAGAG CCATGTGCAC AGATCTTCTT CTGGCCTGTT TCATCTGCCC TGCAATCGTT 
AACCCAGAGC AATATGGGAT CATTTCTGAT GCTCCTATAA ATGAGGTGGC AAGATTTAAT 
CTGATGCAGG TTGGGAGGCT CCTGCAGCAG CTGGCAATGA CAGGCTCTGA GGAGGGAGAT 
CCACGTACCA AGAGCAGCCT TGCTAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTGGAT 
GTGGTCATCG ACGGGCGCGC GGTGGAGACG CCTCCGATGT CTGCTGTCAA CCTGCTGGAG 
GGGCTGAGCA GGACCGTGGT GTACATGACA TACAGCCAGC TGACCACTCT GGTTGGCTTT 
ATGCGCAACG TGATGTCAAG CGATCAGCTT AAGGAAGATC GGATGGCTTT GGAAAACTTG 
CTGGCAAACT TACCCCAGAA CAAGCCAGGG AAAAGCAGCA GCCTTGAAAT GACTCCATAT 
AACACCCCCC AGCTTTCTCC TGCGACCACT CCAGCCAACA AAAAAAACCG GTTACCGATA 
GGACAGCAAT TGGCAGCCAT TACTGCCTGG GACACCTCTG CCACCAATCT GTCAGCTCAC 
ATAACTCTAG TAACCCCTTT TGCAACTCGT AGCAGAAGTA GATCAAATAT TCTGATGGAT 
CAGCACGGAG ATCACGAAGG ATCCTCCCAG GAAACTATCC CAGAAGTTCA GCCAGAAGAA 
GTGCTGGTGA TTTCTCTGGG GACAGGTCCA CAGATTACTC CAGGAATGAT GTCAGAAAAT 
GAGGTCTTAA ACATGCAGCT TGCAGATGGC GGGCAAGATG TCCCCATTGA CGAAAACAAA 
CTCCATGGTA AACCTGATAA AACCTTGCGC TTTTCCCTCT GCAGTGATAA TCTGGAAGGA 
ATATCTGAAG GTCCTTCCAA TCGCTCCAAC TCCGTGTCCT CGCTGGATTT GGAAGGGGAG 
TCGGTGTCGG AGCTCGGCGC GGGCCCCTCC GGGAGCAACG GTGTGGAGGC TCTGCAGCTC 
CTGGAGCATG AACAAGCCAC AACTCAGGAT AATCTGGATG ACAAGCTCCG TAAGTTTGAA 
ATCCGTGATA TGATGGGTTT GACTGATGAC AGAGATATAT CAGAAACTGT GAGTGAAACC 
TGGAGCACAG ATGTCTTGGG AAGTGACTTC GACCCCAACA TGGACGAGGA CCGGCTGCAG 
GAGATCGCAG GTGCAGCTGC AGAGAACATG CTAGGCAGCT TGCTGTGTTT GCCAGGTTCA 
GGATCCGTGT TGCTTGATCC CTGCACAGGT TCAACCATAT CAGAAACCAC AAGTGAGGCG 
TGGAGTGTGG AAGTATTACC GAGTGATTCA GAGGCTCCAG ACTTAAAACA GGAGGAAAGG 
CTCCAAGAAC TGGAGAGCTG TTCTGGGCTG GGCAGCACGT CTGATGACAC AGATGTGAGG 
GAGGTCAGCT CTCGACCCAG CACTCCAGGA CTCAGCGTTG TGTCAGGTAT TAGTGCAACA 
TCTGAAGATA TTCCTAACAA GATTGAGGAT CTCAGATCTG AATGTAGCTC TGACTTTGGG 
GGAAAAGATT CTGTGACAAG TCCTGATATG GATGAAACAG CCCATGGAGC CAGTCAATTG 
ACATCTCCAC CTTCTCAGAC AGATTCTTTG CTTGCACTGT TTGACCCTCT GTCCTCAAAT 
GAGGGTGTGT CAGCTGTAGT AAGGCCTAAA GTGCACTATG CAAGACCCTC TCACCCACCT 
CCGGATCCAC CCATCCTGGA AGGAGCTGTG GGAGGTAACG AGGCCCGACT GCCAAACTTT 
GGGTCTCACG CTCTGATTCC AACCGATCTG GAAGCCTTCA AGCAGAGACA TTCCTACCCG 
GAGAGGCTGG TCCGCAGCCG GAGCTCAGAC ATAGTGTCGT CGGTTCGGAG GCCCATGAGT 
GATCCCGGGT GGAACAGGCG TCCTGGGAAC GAGGACAGGG AGCTGCCCAT GGCCACCAGC 
AGCGCTGGAG CAGCTGTGCT GGCAGCCACG TCTCAGTCAT CGTCTTCATC TCCCAGTAAG 
GACTCCTCCA GGGGAGAGAT TGAGGAACGA AAAGACAGCG ATGATGAGAA GTCTGACAGG 
AACAAGCCCT GGTGGAGGAA ACGTTTTGCG TCTGCCATGC CCAAAGCTCC TATTCCATTT 
AGAAAGAAAG AAAAACAAGA GAAAGACAAA GATGACATGG TGCCTGACAG ATACGCAACA 
CTTCAAGATG ATCCCAGCCC AAGGCTCAGT GCACAGGCAC AAGCTGCTGA GGACATTCTG 
GACAAATATA GGAATGCCAT CAAGAGAACC AGTCCCAGTG AGGGAGCCAT AGTGAACTAT 
GACAGTGCAG AGGCTCTTGG GGATGGTGAG AGCATGCACG ACTCCCCGCG GGACGAGGCC 
TTGCAGAACA TGTCTGCAGA CGATCTCCCA GACTCTGCAA GTCAAGTAGC ACAGCCACAA 
AGTTCTGCTT TCTCCTATAG GGATGCAAAG AAGAAATTGA GATTGGCTCT TTGTTCAGCA 
GATTCTGTTG CCTTCCCGAT GTTGACCCAC TCAACACGGA ATGGTCTCCC AGACCACACA 
GACCCCGAAG ATAATGAAAT TGTGTGCTTC TTGAAAGTTC AGCTGGCTGA AGCCATCAAC 
CTCCAGGACA AGAACCTGAT GGCACAGCTG CAGGAGACGA TGCGCTGCGT GAGCCGCTTC 
GACAACAGGA CCTGTCGGAA GCTGCTGGCC TCCATTGCAG AGGACTATAG GAAAAGAGCT 
CCATATATTG CTTATTTGAC TCGCTGTCGC CAAGGCCTGC AGACGACACA GGCGCACCTG 
GAAAGGCTCT TGCAGAGAGT TCTACGAGAT AAAGAAGTGG CCAACAGATA CTTCACTACA 
GTATGTGTGA GGTTACTGCT GGAGAGCAAA GAAAAGAAAA TAAGGGAATT TATTCAAGAT 
TTCCAGAAGC TCACAGCAGC AGACGATAAA ACAGCCCAAG TTGAGGATTT CCTTCAGTTC 
CTGTACGGGG CTATGGCTCA GGATGCCATA TGGCAGAATG CCAGTGAGGA ACAGCTTCAG 
GATGCACAAT TAGCCATCGA GCGCAGTGTG ATGAATCGCA TTTTCAAACT TGCCTTCTAC 
CCTAATCAGG ATGGAGATAT TCTGCGTGAC CAGGTCCTTC ACGAGCACAT ACAGAGGTTA 
TCCAAAGTAG TGACTGCAAA CCACAAGGCC CTTCAGATAC CTGAGGTGTA TCTCCGGGAG 
GCACCGTGGC CGTCGGCACA GTCCGAGATC CGCACGATAA GTGCTTACAA AACCCCCCGG 
GACAAGGTGC AGTGTATCCT GAGGATGTGC TCCACCATCA TGAACCTGCT CAGCCTGGCC 
AACGAGGATT CAGTACCTGG GGCAGATGAT TTTGTTCCTG TTTTGGTTTT TGTCCTGATA 
AAGGCAAACC CCCCTTGCCT GCTGTCCACT GTGCAGTACA TCAGTAGTTT CTATGCCAAC 
TGCTTGTCTG GAGAGGAGTC GTACTGGTGG ATGCAGTTCA CGGCAGCCGT GGAGTTCATC 
AAAACCATCG ATGATCGCAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPVD1 ( XM_017810048.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017665537.1 Lepidothrix coronata GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X3, mRNA.

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CloneID	OLe54931
Clone ID Related Accession (Same CDS sequence)	XM_017810046.1 , XM_017810047.1 , XM_017810048.1
Accession Version	XM_017810048.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690208.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGC TGGACATCCA CACGCTGGCC CACCACCTGA AGCAGGAGCG GCTCTACGTG 
ACGGCGGAGA AGCAGCTGAT CCAGCGGCTC AACGCCGACG TGCTGAAGAC GGCGGAGAAG 
CTCTACCGGA CAGCCTGGAT CTCCAAACAG CAGCGCATCA ACCTGGACAG GCTCATCATC 
ACCAGTGCTG AAGCTTCTCC TGCTGAATGT TGCCAACATG CAAAAATCTT GGAGGACACA 
CAGTTTGTGG ATGGATATAA GCAGCTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCCTG 
AACAGACTGA GAGAGAACCC GAGGCTTATT GCATCCTGCC TGGTTGCTGG AGAGAAGCTC 
AACCAGGACA ACACTCAGAG TGTCATTCAC ACAGTCTTTA CCTCCATTTA TGGCAACTGC 
ATCATGCAGG AGGATGAGAG TTACCTGCTC CAGGTCCTCC GTTACCTGAT TGAGTTTGAA 
CTCAAGGAGA GCGACAACCC CCGGCGGCTG CTCCGACGAG GCACCTGTGC CTTCAGCATC 
TTGTTCAAAC TCTTCTCTGA AGGACTCTTC TCTGCAAAAC TTTTTCTTAC TGCAACCTTA 
CATGAGCCAA TCATGCAGCT CCTGGTTGAA GATGAAGACC ACCTGGAAAC CGATGCAAAC 
AAGTTAATTG AGAGATTCTC CCCAGTACAG CAGGAAAAGT TATTTGGAGA GAAAGGCACA 
GAAAAGTTCA AGCAAAGAGT CCAAGAGATG GTTGACTCCA ACGAGGCCAA GCTGGTGACT 
TTGGTCAACA AATTCATTGG CTATCTCAAA CAAAACACTT ACTGTTTTCC TCACAGCTTG 
AGGTGGATTG TGTCGCAGAT GTACAAAACG CTGTCGTGTG TGGACAGGCT GGAGGTTGGG 
GAGGTCAGAG CCATGTGCAC AGATCTTCTT CTGGCCTGTT TCATCTGCCC TGCAATCGTT 
AACCCAGAGC AATATGGGAT CATTTCTGAT GCTCCTATAA ATGAGGTGGC AAGATTTAAT 
CTGATGCAGG TTGGGAGGCT CCTGCAGCAG CTGGCAATGA CAGGCTCTGA GGAGGGAGAT 
CCACGTACCA AGAGCAGCCT TGCTAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTGGAT 
GTGGTCATCG ACGGGCGCGC GGTGGAGACG CCTCCGATGT CTGCTGTCAA CCTGCTGGAG 
GGGCTGAGCA GGACCGTGGT GTACATGACA TACAGCCAGC TGACCACTCT GGTTGGCTTT 
ATGCGCAACG TGATGTCAAG CGATCAGCTT AAGGAAGATC GGATGGCTTT GGAAAACTTG 
CTGGCAAACT TACCCCAGAA CAAGCCAGGG AAAAGCAGCA GCCTTGAAAT GACTCCATAT 
AACACCCCCC AGCTTTCTCC TGCGACCACT CCAGCCAACA AAAAAAACCG GTTACCGATA 
GGACAGCAAT TGGCAGCCAT TACTGCCTGG GACACCTCTG CCACCAATCT GTCAGCTCAC 
ATAACTCTAG TAACCCCTTT TGCAACTCGT AGCAGAAGTA GATCAAATAT TCTGATGGAT 
CAGCACGGAG ATCACGAAGG ATCCTCCCAG GAAACTATCC CAGAAGTTCA GCCAGAAGAA 
GTGCTGGTGA TTTCTCTGGG GACAGGTCCA CAGATTACTC CAGGAATGAT GTCAGAAAAT 
GAGGTCTTAA ACATGCAGCT TGCAGATGGC GGGCAAGATG TCCCCATTGA CGAAAACAAA 
CTCCATGGTA AACCTGATAA AACCTTGCGC TTTTCCCTCT GCAGTGATAA TCTGGAAGGA 
ATATCTGAAG GTCCTTCCAA TCGCTCCAAC TCCGTGTCCT CGCTGGATTT GGAAGGGGAG 
TCGGTGTCGG AGCTCGGCGC GGGCCCCTCC GGGAGCAACG GTGTGGAGGC TCTGCAGCTC 
CTGGAGCATG AACAAGCCAC AACTCAGGAT AATCTGGATG ACAAGCTCCG TAAGTTTGAA 
ATCCGTGATA TGATGGGTTT GACTGATGAC AGAGATATAT CAGAAACTGT GAGTGAAACC 
TGGAGCACAG ATGTCTTGGG AAGTGACTTC GACCCCAACA TGGACGAGGA CCGGCTGCAG 
GAGATCGCAG GTGCAGCTGC AGAGAACATG CTAGGCAGCT TGCTGTGTTT GCCAGGTTCA 
GGATCCGTGT TGCTTGATCC CTGCACAGGT TCAACCATAT CAGAAACCAC AAGTGAGGCG 
TGGAGTGTGG AAGTATTACC GAGTGATTCA GAGGCTCCAG ACTTAAAACA GGAGGAAAGG 
CTCCAAGAAC TGGAGAGCTG TTCTGGGCTG GGCAGCACGT CTGATGACAC AGATGTGAGG 
GAGGTCAGCT CTCGACCCAG CACTCCAGGA CTCAGCGTTG TGTCAGGTAT TAGTGCAACA 
TCTGAAGATA TTCCTAACAA GATTGAGGAT CTCAGATCTG AATGTAGCTC TGACTTTGGG 
GGAAAAGATT CTGTGACAAG TCCTGATATG GATGAAACAG CCCATGGAGC CAGTCAATTG 
ACATCTCCAC CTTCTCAGAC AGATTCTTTG CTTGCACTGT TTGACCCTCT GTCCTCAAAT 
GAGGGTGTGT CAGCTGTAGT AAGGCCTAAA GTGCACTATG CAAGACCCTC TCACCCACCT 
CCGGATCCAC CCATCCTGGA AGGAGCTGTG GGAGGTAACG AGGCCCGACT GCCAAACTTT 
GGGTCTCACG CTCTGATTCC AACCGATCTG GAAGCCTTCA AGCAGAGACA TTCCTACCCG 
GAGAGGCTGG TCCGCAGCCG GAGCTCAGAC ATAGTGTCGT CGGTTCGGAG GCCCATGAGT 
GATCCCGGGT GGAACAGGCG TCCTGGGAAC GAGGACAGGG AGCTGCCCAT GGCCACCAGC 
AGCGCTGGAG CAGCTGTGCT GGCAGCCACG TCTCAGTCAT CGTCTTCATC TCCCAGTAAG 
GACTCCTCCA GGGGAGAGAT TGAGGAACGA AAAGACAGCG ATGATGAGAA GTCTGACAGG 
AACAAGCCCT GGTGGAGGAA ACGTTTTGCG TCTGCCATGC CCAAAGCTCC TATTCCATTT 
AGAAAGAAAG AAAAACAAGA GAAAGACAAA GATGACATGG TGCCTGACAG ATACGCAACA 
CTTCAAGATG ATCCCAGCCC AAGGCTCAGT GCACAGGCAC AAGCTGCTGA GGACATTCTG 
GACAAATATA GGAATGCCAT CAAGAGAACC AGTCCCAGTG AGGGAGCCAT AGTGAACTAT 
GACAGTGCAG AGGCTCTTGG GGATGGTGAG AGCATGCACG ACTCCCCGCG GGACGAGGCC 
TTGCAGAACA TGTCTGCAGA CGATCTCCCA GACTCTGCAA GTCAAGTAGC ACAGCCACAA 
AGTTCTGCTT TCTCCTATAG GGATGCAAAG AAGAAATTGA GATTGGCTCT TTGTTCAGCA 
GATTCTGTTG CCTTCCCGAT GTTGACCCAC TCAACACGGA ATGGTCTCCC AGACCACACA 
GACCCCGAAG ATAATGAAAT TGTGTGCTTC TTGAAAGTTC AGCTGGCTGA AGCCATCAAC 
CTCCAGGACA AGAACCTGAT GGCACAGCTG CAGGAGACGA TGCGCTGCGT GAGCCGCTTC 
GACAACAGGA CCTGTCGGAA GCTGCTGGCC TCCATTGCAG AGGACTATAG GAAAAGAGCT 
CCATATATTG CTTATTTGAC TCGCTGTCGC CAAGGCCTGC AGACGACACA GGCGCACCTG 
GAAAGGCTCT TGCAGAGAGT TCTACGAGAT AAAGAAGTGG CCAACAGATA CTTCACTACA 
GTATGTGTGA GGTTACTGCT GGAGAGCAAA GAAAAGAAAA TAAGGGAATT TATTCAAGAT 
TTCCAGAAGC TCACAGCAGC AGACGATAAA ACAGCCCAAG TTGAGGATTT CCTTCAGTTC 
CTGTACGGGG CTATGGCTCA GGATGCCATA TGGCAGAATG CCAGTGAGGA ACAGCTTCAG 
GATGCACAAT TAGCCATCGA GCGCAGTGTG ATGAATCGCA TTTTCAAACT TGCCTTCTAC 
CCTAATCAGG ATGGAGATAT TCTGCGTGAC CAGGTCCTTC ACGAGCACAT ACAGAGGTTA 
TCCAAAGTAG TGACTGCAAA CCACAAGGCC CTTCAGATAC CTGAGGTGTA TCTCCGGGAG 
GCACCGTGGC CGTCGGCACA GTCCGAGATC CGCACGATAA GTGCTTACAA AACCCCCCGG 
GACAAGGTGC AGTGTATCCT GAGGATGTGC TCCACCATCA TGAACCTGCT CAGCCTGGCC 
AACGAGGATT CAGTACCTGG GGCAGATGAT TTTGTTCCTG TTTTGGTTTT TGTCCTGATA 
AAGGCAAACC CCCCTTGCCT GCTGTCCACT GTGCAGTACA TCAGTAGTTT CTATGCCAAC 
TGCTTGTCTG GAGAGGAGTC GTACTGGTGG ATGCAGTTCA CGGCAGCCGT GGAGTTCATC 
AAAACCATCG ATGATCGCAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017665537.1
CDS	246..4709
Translation	MVKLDIHTLAHHLKQERLYVTAEKQLIQRLNADVLKTAEKLYRTAWISKQQRINLDRLIITSAEASPAECCQHAKILEDTQFVDGYKQLGFQETAYGEFLNRLRENPRLIASCLVAGEKLNQDNTQSVIHTVFTSIYGNCIMQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDANKLIERFSPVQQEKLFGEKGTEKFKQRVQEMVDSNEAKLVTLVNKFIGYLKQNTYCFPHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAIVNPEQYGIISDAPINEVARFNLMQVGRLLQQLAMTGSEEGDPRTKSSLAKFDKSCVAAFLDVVIDGRAVETPPMSAVNLLEGLSRTVVYMTYSQLTTLVGFMRNVMSSDQLKEDRMALENLLANLPQNKPGKSSSLEMTPYNTPQLSPATTPANKKNRLPIGQQLAAITAWDTSATNLSAHITLVTPFATRSRSRSNILMDQHGDHEGSSQETIPEVQPEEVLVISLGTGPQITPGMMSENEVLNMQLADGGQDVPIDENKLHGKPDKTLRFSLCSDNLEGISEGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNMDEDRLQEIAGAAAENMLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDETAHGASQLTSPPSQTDSLLALFDPLSSNEGVSAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHALIPTDLEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPGWNRRPGNEDRELPMATSSAGAAVLAATSQSSSSSPSKDSSRGEIEERKDSDDEKSDRNKPWWRKRFASAMPKAPIPFRKKEKQEKDKDDMVPDRYATLQDDPSPRLSAQAQAAEDILDKYRNAIKRTSPSEGAIVNYDSAEALGDGESMHDSPRDEALQNMSADDLPDSASQVAQPQSSAFSYRDAKKKLRLALCSADSVAFPMLTHSTRNGLPDHTDPEDNEIVCFLKVQLAEAINLQDKNLMAQLQETMRCVSRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGAMAQDAIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHKALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYANCLSGEESYWWMQFTAAVEFIKTIDDRK

Target ORF information:

RefSeq Version	XM_017810048.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017810048.1

ORF Insert Sequence:

ATGGTGAAGC TGGACATCCA CACGCTGGCC CACCACCTGA AGCAGGAGCG GCTCTACGTG 
ACGGCGGAGA AGCAGCTGAT CCAGCGGCTC AACGCCGACG TGCTGAAGAC GGCGGAGAAG 
CTCTACCGGA CAGCCTGGAT CTCCAAACAG CAGCGCATCA ACCTGGACAG GCTCATCATC 
ACCAGTGCTG AAGCTTCTCC TGCTGAATGT TGCCAACATG CAAAAATCTT GGAGGACACA 
CAGTTTGTGG ATGGATATAA GCAGCTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCCTG 
AACAGACTGA GAGAGAACCC GAGGCTTATT GCATCCTGCC TGGTTGCTGG AGAGAAGCTC 
AACCAGGACA ACACTCAGAG TGTCATTCAC ACAGTCTTTA CCTCCATTTA TGGCAACTGC 
ATCATGCAGG AGGATGAGAG TTACCTGCTC CAGGTCCTCC GTTACCTGAT TGAGTTTGAA 
CTCAAGGAGA GCGACAACCC CCGGCGGCTG CTCCGACGAG GCACCTGTGC CTTCAGCATC 
TTGTTCAAAC TCTTCTCTGA AGGACTCTTC TCTGCAAAAC TTTTTCTTAC TGCAACCTTA 
CATGAGCCAA TCATGCAGCT CCTGGTTGAA GATGAAGACC ACCTGGAAAC CGATGCAAAC 
AAGTTAATTG AGAGATTCTC CCCAGTACAG CAGGAAAAGT TATTTGGAGA GAAAGGCACA 
GAAAAGTTCA AGCAAAGAGT CCAAGAGATG GTTGACTCCA ACGAGGCCAA GCTGGTGACT 
TTGGTCAACA AATTCATTGG CTATCTCAAA CAAAACACTT ACTGTTTTCC TCACAGCTTG 
AGGTGGATTG TGTCGCAGAT GTACAAAACG CTGTCGTGTG TGGACAGGCT GGAGGTTGGG 
GAGGTCAGAG CCATGTGCAC AGATCTTCTT CTGGCCTGTT TCATCTGCCC TGCAATCGTT 
AACCCAGAGC AATATGGGAT CATTTCTGAT GCTCCTATAA ATGAGGTGGC AAGATTTAAT 
CTGATGCAGG TTGGGAGGCT CCTGCAGCAG CTGGCAATGA CAGGCTCTGA GGAGGGAGAT 
CCACGTACCA AGAGCAGCCT TGCTAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTGGAT 
GTGGTCATCG ACGGGCGCGC GGTGGAGACG CCTCCGATGT CTGCTGTCAA CCTGCTGGAG 
GGGCTGAGCA GGACCGTGGT GTACATGACA TACAGCCAGC TGACCACTCT GGTTGGCTTT 
ATGCGCAACG TGATGTCAAG CGATCAGCTT AAGGAAGATC GGATGGCTTT GGAAAACTTG 
CTGGCAAACT TACCCCAGAA CAAGCCAGGG AAAAGCAGCA GCCTTGAAAT GACTCCATAT 
AACACCCCCC AGCTTTCTCC TGCGACCACT CCAGCCAACA AAAAAAACCG GTTACCGATA 
GGACAGCAAT TGGCAGCCAT TACTGCCTGG GACACCTCTG CCACCAATCT GTCAGCTCAC 
ATAACTCTAG TAACCCCTTT TGCAACTCGT AGCAGAAGTA GATCAAATAT TCTGATGGAT 
CAGCACGGAG ATCACGAAGG ATCCTCCCAG GAAACTATCC CAGAAGTTCA GCCAGAAGAA 
GTGCTGGTGA TTTCTCTGGG GACAGGTCCA CAGATTACTC CAGGAATGAT GTCAGAAAAT 
GAGGTCTTAA ACATGCAGCT TGCAGATGGC GGGCAAGATG TCCCCATTGA CGAAAACAAA 
CTCCATGGTA AACCTGATAA AACCTTGCGC TTTTCCCTCT GCAGTGATAA TCTGGAAGGA 
ATATCTGAAG GTCCTTCCAA TCGCTCCAAC TCCGTGTCCT CGCTGGATTT GGAAGGGGAG 
TCGGTGTCGG AGCTCGGCGC GGGCCCCTCC GGGAGCAACG GTGTGGAGGC TCTGCAGCTC 
CTGGAGCATG AACAAGCCAC AACTCAGGAT AATCTGGATG ACAAGCTCCG TAAGTTTGAA 
ATCCGTGATA TGATGGGTTT GACTGATGAC AGAGATATAT CAGAAACTGT GAGTGAAACC 
TGGAGCACAG ATGTCTTGGG AAGTGACTTC GACCCCAACA TGGACGAGGA CCGGCTGCAG 
GAGATCGCAG GTGCAGCTGC AGAGAACATG CTAGGCAGCT TGCTGTGTTT GCCAGGTTCA 
GGATCCGTGT TGCTTGATCC CTGCACAGGT TCAACCATAT CAGAAACCAC AAGTGAGGCG 
TGGAGTGTGG AAGTATTACC GAGTGATTCA GAGGCTCCAG ACTTAAAACA GGAGGAAAGG 
CTCCAAGAAC TGGAGAGCTG TTCTGGGCTG GGCAGCACGT CTGATGACAC AGATGTGAGG 
GAGGTCAGCT CTCGACCCAG CACTCCAGGA CTCAGCGTTG TGTCAGGTAT TAGTGCAACA 
TCTGAAGATA TTCCTAACAA GATTGAGGAT CTCAGATCTG AATGTAGCTC TGACTTTGGG 
GGAAAAGATT CTGTGACAAG TCCTGATATG GATGAAACAG CCCATGGAGC CAGTCAATTG 
ACATCTCCAC CTTCTCAGAC AGATTCTTTG CTTGCACTGT TTGACCCTCT GTCCTCAAAT 
GAGGGTGTGT CAGCTGTAGT AAGGCCTAAA GTGCACTATG CAAGACCCTC TCACCCACCT 
CCGGATCCAC CCATCCTGGA AGGAGCTGTG GGAGGTAACG AGGCCCGACT GCCAAACTTT 
GGGTCTCACG CTCTGATTCC AACCGATCTG GAAGCCTTCA AGCAGAGACA TTCCTACCCG 
GAGAGGCTGG TCCGCAGCCG GAGCTCAGAC ATAGTGTCGT CGGTTCGGAG GCCCATGAGT 
GATCCCGGGT GGAACAGGCG TCCTGGGAAC GAGGACAGGG AGCTGCCCAT GGCCACCAGC 
AGCGCTGGAG CAGCTGTGCT GGCAGCCACG TCTCAGTCAT CGTCTTCATC TCCCAGTAAG 
GACTCCTCCA GGGGAGAGAT TGAGGAACGA AAAGACAGCG ATGATGAGAA GTCTGACAGG 
AACAAGCCCT GGTGGAGGAA ACGTTTTGCG TCTGCCATGC CCAAAGCTCC TATTCCATTT 
AGAAAGAAAG AAAAACAAGA GAAAGACAAA GATGACATGG TGCCTGACAG ATACGCAACA 
CTTCAAGATG ATCCCAGCCC AAGGCTCAGT GCACAGGCAC AAGCTGCTGA GGACATTCTG 
GACAAATATA GGAATGCCAT CAAGAGAACC AGTCCCAGTG AGGGAGCCAT AGTGAACTAT 
GACAGTGCAG AGGCTCTTGG GGATGGTGAG AGCATGCACG ACTCCCCGCG GGACGAGGCC 
TTGCAGAACA TGTCTGCAGA CGATCTCCCA GACTCTGCAA GTCAAGTAGC ACAGCCACAA 
AGTTCTGCTT TCTCCTATAG GGATGCAAAG AAGAAATTGA GATTGGCTCT TTGTTCAGCA 
GATTCTGTTG CCTTCCCGAT GTTGACCCAC TCAACACGGA ATGGTCTCCC AGACCACACA 
GACCCCGAAG ATAATGAAAT TGTGTGCTTC TTGAAAGTTC AGCTGGCTGA AGCCATCAAC 
CTCCAGGACA AGAACCTGAT GGCACAGCTG CAGGAGACGA TGCGCTGCGT GAGCCGCTTC 
GACAACAGGA CCTGTCGGAA GCTGCTGGCC TCCATTGCAG AGGACTATAG GAAAAGAGCT 
CCATATATTG CTTATTTGAC TCGCTGTCGC CAAGGCCTGC AGACGACACA GGCGCACCTG 
GAAAGGCTCT TGCAGAGAGT TCTACGAGAT AAAGAAGTGG CCAACAGATA CTTCACTACA 
GTATGTGTGA GGTTACTGCT GGAGAGCAAA GAAAAGAAAA TAAGGGAATT TATTCAAGAT 
TTCCAGAAGC TCACAGCAGC AGACGATAAA ACAGCCCAAG TTGAGGATTT CCTTCAGTTC 
CTGTACGGGG CTATGGCTCA GGATGCCATA TGGCAGAATG CCAGTGAGGA ACAGCTTCAG 
GATGCACAAT TAGCCATCGA GCGCAGTGTG ATGAATCGCA TTTTCAAACT TGCCTTCTAC 
CCTAATCAGG ATGGAGATAT TCTGCGTGAC CAGGTCCTTC ACGAGCACAT ACAGAGGTTA 
TCCAAAGTAG TGACTGCAAA CCACAAGGCC CTTCAGATAC CTGAGGTGTA TCTCCGGGAG 
GCACCGTGGC CGTCGGCACA GTCCGAGATC CGCACGATAA GTGCTTACAA AACCCCCCGG 
GACAAGGTGC AGTGTATCCT GAGGATGTGC TCCACCATCA TGAACCTGCT CAGCCTGGCC 
AACGAGGATT CAGTACCTGG GGCAGATGAT TTTGTTCCTG TTTTGGTTTT TGTCCTGATA 
AAGGCAAACC CCCCTTGCCT GCTGTCCACT GTGCAGTACA TCAGTAGTTT CTATGCCAAC 
TGCTTGTCTG GAGAGGAGTC GTACTGGTGG ATGCAGTTCA CGGCAGCCGT GGAGTTCATC 
AAAACCATCG ATGATCGCAA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPVD1 ( XM_017810049.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017665538.1 Lepidothrix coronata GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X4, mRNA.

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CloneID	OLe54932
Clone ID Related Accession (Same CDS sequence)	XM_017810049.1
Accession Version	XM_017810049.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690208.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGC TGGACATCCA CACGCTGGCC CACCACCTGA AGCAGGAGCG GCTCTACGTG 
ACGGCGGAGA AGCAGCTGAT CCAGCGGCTC AACGCCGACG TGCTGAAGAC GGCGGAGAAG 
CTCTACCGGA CAGCCTGGAT CTCCAAACAG CAGCGCATCA ACCTGGACAG GCTCATCATC 
ACCAGTGCTG AAGCTTCTCC TGCTGAATGT TGCCAACATG CAAAAATCTT GGAGGACACA 
CAGTTTGTGG ATGGATATAA GCAGCTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCCTG 
AACAGACTGA GAGAGAACCC GAGGCTTATT GCATCCTGCC TGGTTGCTGG AGAGAAGCTC 
AACCAGGACA ACACTCAGAG TGTCATTCAC ACAGTCTTTA CCTCCATTTA TGGCAACTGC 
ATCATGCAGG AGGATGAGAG TTACCTGCTC CAGGTCCTCC GTTACCTGAT TGAGTTTGAA 
CTCAAGGAGA GCGACAACCC CCGGCGGCTG CTCCGACGAG GCACCTGTGC CTTCAGCATC 
TTGTTCAAAC TCTTCTCTGA AGGACTCTTC TCTGCAAAAC TTTTTCTTAC TGCAACCTTA 
CATGAGCCAA TCATGCAGCT CCTGGTTGAA GATGAAGACC ACCTGGAAAC CGATGCAAAC 
AAGTTAATTG AGAGATTCTC CCCAGTACAG CAGGAAAAGT TATTTGGAGA GAAAGGCACA 
GAAAAGTTCA AGCAAAGAGT CCAAGAGATG GTTGACTCCA ACGAGGCCAA GCTGGTGACT 
TTGGTCAACA AATTCATTGG CTATCTCAAA CAAAACACTT ACTGTTTTCC TCACAGCTTG 
AGGTGGATTG TGTCGCAGAT GTACAAAACG CTGTCGTGTG TGGACAGGCT GGAGGTTGGG 
GAGGTCAGAG CCATGTGCAC AGATCTTCTT CTGGCCTGTT TCATCTGCCC TGCAATCGTT 
AACCCAGAGC AATATGGGAT CATTTCTGAT GCTCCTATAA ATGAGGTGGC AAGATTTAAT 
CTGATGCAGG TTGGGAGGCT CCTGCAGCAG CTGGCAATGA CAGGCTCTGA GGAGGGAGAT 
CCACGTACCA AGAGCAGCCT TGCTAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTGGAT 
GTGGTCATCG ACGGGCGCGC GGTGGAGACG CCTCCGATGT CTGCTGTCAA CCTGCTGGAG 
GGGCTGAGCA GGACCGTGGT GTACATGACA TACAGCCAGC TGACCACTCT GGTTGGCTTT 
ATGCGCAACG TGATGTCAAG CGATCAGCTT AAGGAAGATC GGATGGCTTT GGAAAACTTG 
CTGGCAAACT TACCCCAGAA CAAGCCAGGG AAAAGCAGCA GCCTTGAAAT GACTCCATAT 
AACACCCCCC AGCTTTCTCC TGCGACCACT CCAGCCAACA AAAAAAACCG GTTACCGATA 
GGACAGCAAT TGGCAGCCAT TACTGCCTGG GACACCTCTG CCACCAATCT GTCAGCTCAC 
ATAACTCTAG TAACCCCTTT TGCAACTCGT AGCAGAAGTA GATCAAATAT TCTGATGGAT 
CAGCACGGAG ATCACGAAGG ATCCTCCCAG GAAACTATCC CAGAAGTTCA GCCAGAAGAA 
GTGCTGGTGA TTTCTCTGGG GACAGGTCCA CAGATTACTC CAGGAATGAT GTCAGAAAAT 
GAGGTCTTAA ACATGCAGCT TGCAGATGGC GGGCAAGATG TCCCCATTGA CGAAAACAAA 
CTCCATGGTC CTTCCAATCG CTCCAACTCC GTGTCCTCGC TGGATTTGGA AGGGGAGTCG 
GTGTCGGAGC TCGGCGCGGG CCCCTCCGGG AGCAACGGTG TGGAGGCTCT GCAGCTCCTG 
GAGCATGAAC AAGCCACAAC TCAGGATAAT CTGGATGACA AGCTCCGTAA GTTTGAAATC 
CGTGATATGA TGGGTTTGAC TGATGACAGA GATATATCAG AAACTGTGAG TGAAACCTGG 
AGCACAGATG TCTTGGGAAG TGACTTCGAC CCCAACATGG ACGAGGACCG GCTGCAGGAG 
ATCGCAGGTG CAGCTGCAGA GAACATGCTA GGCAGCTTGC TGTGTTTGCC AGGTTCAGGA 
TCCGTGTTGC TTGATCCCTG CACAGGTTCA ACCATATCAG AAACCACAAG TGAGGCGTGG 
AGTGTGGAAG TATTACCGAG TGATTCAGAG GCTCCAGACT TAAAACAGGA GGAAAGGCTC 
CAAGAACTGG AGAGCTGTTC TGGGCTGGGC AGCACGTCTG ATGACACAGA TGTGAGGGAG 
GTCAGCTCTC GACCCAGCAC TCCAGGACTC AGCGTTGTGT CAGGTATTAG TGCAACATCT 
GAAGATATTC CTAACAAGAT TGAGGATCTC AGATCTGAAT GTAGCTCTGA CTTTGGGGGA 
AAAGATTCTG TGACAAGTCC TGATATGGAT GAAACAGCCC ATGGAGCCAG TCAATTGACA 
TCTCCACCTT CTCAGACAGA TTCTTTGCTT GCACTGTTTG ACCCTCTGTC CTCAAATGAG 
GGTGTGTCAG CTGTAGTAAG GCCTAAAGTG CACTATGCAA GACCCTCTCA CCCACCTCCG 
GATCCACCCA TCCTGGAAGG AGCTGTGGGA GGTAACGAGG CCCGACTGCC AAACTTTGGG 
TCTCACGCTC TGATTCCAAC CGATCTGGAA GCCTTCAAGC AGAGACATTC CTACCCGGAG 
AGGCTGGTCC GCAGCCGGAG CTCAGACATA GTGTCGTCGG TTCGGAGGCC CATGAGTGAT 
CCCGGGTGGA ACAGGCGTCC TGGGAACGAG GACAGGGAGC TGCCCATGGC CACCAGCAGC 
GCTGGAGCAG CTGTGCTGGC AGCCACGTCT CAGTCATCGT CTTCATCTCC CAGTAAGGAC 
TCCTCCAGGG GAGAGATTGA GGAACGAAAA GACAGCGATG ATGAGAAGTC TGACAGGAAC 
AAGCCCTGGT GGAGGAAACG TTTTGCGTCT GCCATGCCCA AAGCTCCTAT TCCATTTAGA 
AAGAAAGAAA AACAAGAGAA AGACAAAGAT GACATGGTGC CTGACAGATA CGCAACACTT 
CAAGATGATC CCAGCCCAAG GCTCAGTGCA CAGGCACAAG CTGCTGAGGA CATTCTGGAC 
AAATATAGGA ATGCCATCAA GAGAACCAGT CCCAGTGAGG GAGCCATAGT GAACTATGAC 
AGTGCAGAGG CTCTTGGGGA TGGTGAGAGC ATGCACGACT CCCCGCGGGA CGAGGCCTTG 
CAGAACATGT CTGCAGACGA TCTCCCAGAC TCTGCAAGTC AAGTAGCACA GCCACAAAGT 
TCTGCTTTCT CCTATAGGGA TGCAAAGAAG AAATTGAGAT TGGCTCTTTG TTCAGCAGAT 
TCTGTTGCCT TCCCGATGTT GACCCACTCA ACACGGAATG GTCTCCCAGA CCACACAGAC 
CCCGAAGATA ATGAAATTGT GTGCTTCTTG AAAGTTCAGC TGGCTGAAGC CATCAACCTC 
CAGGACAAGA ACCTGATGGC ACAGCTGCAG GAGACGATGC GCTGCGTGAG CCGCTTCGAC 
AACAGGACCT GTCGGAAGCT GCTGGCCTCC ATTGCAGAGG ACTATAGGAA AAGAGCTCCA 
TATATTGCTT ATTTGACTCG CTGTCGCCAA GGCCTGCAGA CGACACAGGC GCACCTGGAA 
AGGCTCTTGC AGAGAGTTCT ACGAGATAAA GAAGTGGCCA ACAGATACTT CACTACAGTA 
TGTGTGAGGT TACTGCTGGA GAGCAAAGAA AAGAAAATAA GGGAATTTAT TCAAGATTTC 
CAGAAGCTCA CAGCAGCAGA CGATAAAACA GCCCAAGTTG AGGATTTCCT TCAGTTCCTG 
TACGGGGCTA TGGCTCAGGA TGCCATATGG CAGAATGCCA GTGAGGAACA GCTTCAGGAT 
GCACAATTAG CCATCGAGCG CAGTGTGATG AATCGCATTT TCAAACTTGC CTTCTACCCT 
AATCAGGATG GAGATATTCT GCGTGACCAG GTCCTTCACG AGCACATACA GAGGTTATCC 
AAAGTAGTGA CTGCAAACCA CAAGGCCCTT CAGATACCTG AGGTGTATCT CCGGGAGGCA 
CCGTGGCCGT CGGCACAGTC CGAGATCCGC ACGATAAGTG CTTACAAAAC CCCCCGGGAC 
AAGGTGCAGT GTATCCTGAG GATGTGCTCC ACCATCATGA ACCTGCTCAG CCTGGCCAAC 
GAGGATTCAG TACCTGGGGC AGATGATTTT GTTCCTGTTT TGGTTTTTGT CCTGATAAAG 
GCAAACCCCC CTTGCCTGCT GTCCACTGTG CAGTACATCA GTAGTTTCTA TGCCAACTGC 
TTGTCTGGAG AGGAGTCGTA CTGGTGGATG CAGTTCACGG CAGCCGTGGA GTTCATCAAA 
ACCATCGATG ATCGCAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017665538.1
CDS	162..4562
Translation	MVKLDIHTLAHHLKQERLYVTAEKQLIQRLNADVLKTAEKLYRTAWISKQQRINLDRLIITSAEASPAECCQHAKILEDTQFVDGYKQLGFQETAYGEFLNRLRENPRLIASCLVAGEKLNQDNTQSVIHTVFTSIYGNCIMQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDANKLIERFSPVQQEKLFGEKGTEKFKQRVQEMVDSNEAKLVTLVNKFIGYLKQNTYCFPHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAIVNPEQYGIISDAPINEVARFNLMQVGRLLQQLAMTGSEEGDPRTKSSLAKFDKSCVAAFLDVVIDGRAVETPPMSAVNLLEGLSRTVVYMTYSQLTTLVGFMRNVMSSDQLKEDRMALENLLANLPQNKPGKSSSLEMTPYNTPQLSPATTPANKKNRLPIGQQLAAITAWDTSATNLSAHITLVTPFATRSRSRSNILMDQHGDHEGSSQETIPEVQPEEVLVISLGTGPQITPGMMSENEVLNMQLADGGQDVPIDENKLHGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNMDEDRLQEIAGAAAENMLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDETAHGASQLTSPPSQTDSLLALFDPLSSNEGVSAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHALIPTDLEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPGWNRRPGNEDRELPMATSSAGAAVLAATSQSSSSSPSKDSSRGEIEERKDSDDEKSDRNKPWWRKRFASAMPKAPIPFRKKEKQEKDKDDMVPDRYATLQDDPSPRLSAQAQAAEDILDKYRNAIKRTSPSEGAIVNYDSAEALGDGESMHDSPRDEALQNMSADDLPDSASQVAQPQSSAFSYRDAKKKLRLALCSADSVAFPMLTHSTRNGLPDHTDPEDNEIVCFLKVQLAEAINLQDKNLMAQLQETMRCVSRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGAMAQDAIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHKALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYANCLSGEESYWWMQFTAAVEFIKTIDDRK

Target ORF information:

RefSeq Version	XM_017810049.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017810049.1

ORF Insert Sequence:

ATGGTGAAGC TGGACATCCA CACGCTGGCC CACCACCTGA AGCAGGAGCG GCTCTACGTG 
ACGGCGGAGA AGCAGCTGAT CCAGCGGCTC AACGCCGACG TGCTGAAGAC GGCGGAGAAG 
CTCTACCGGA CAGCCTGGAT CTCCAAACAG CAGCGCATCA ACCTGGACAG GCTCATCATC 
ACCAGTGCTG AAGCTTCTCC TGCTGAATGT TGCCAACATG CAAAAATCTT GGAGGACACA 
CAGTTTGTGG ATGGATATAA GCAGCTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCCTG 
AACAGACTGA GAGAGAACCC GAGGCTTATT GCATCCTGCC TGGTTGCTGG AGAGAAGCTC 
AACCAGGACA ACACTCAGAG TGTCATTCAC ACAGTCTTTA CCTCCATTTA TGGCAACTGC 
ATCATGCAGG AGGATGAGAG TTACCTGCTC CAGGTCCTCC GTTACCTGAT TGAGTTTGAA 
CTCAAGGAGA GCGACAACCC CCGGCGGCTG CTCCGACGAG GCACCTGTGC CTTCAGCATC 
TTGTTCAAAC TCTTCTCTGA AGGACTCTTC TCTGCAAAAC TTTTTCTTAC TGCAACCTTA 
CATGAGCCAA TCATGCAGCT CCTGGTTGAA GATGAAGACC ACCTGGAAAC CGATGCAAAC 
AAGTTAATTG AGAGATTCTC CCCAGTACAG CAGGAAAAGT TATTTGGAGA GAAAGGCACA 
GAAAAGTTCA AGCAAAGAGT CCAAGAGATG GTTGACTCCA ACGAGGCCAA GCTGGTGACT 
TTGGTCAACA AATTCATTGG CTATCTCAAA CAAAACACTT ACTGTTTTCC TCACAGCTTG 
AGGTGGATTG TGTCGCAGAT GTACAAAACG CTGTCGTGTG TGGACAGGCT GGAGGTTGGG 
GAGGTCAGAG CCATGTGCAC AGATCTTCTT CTGGCCTGTT TCATCTGCCC TGCAATCGTT 
AACCCAGAGC AATATGGGAT CATTTCTGAT GCTCCTATAA ATGAGGTGGC AAGATTTAAT 
CTGATGCAGG TTGGGAGGCT CCTGCAGCAG CTGGCAATGA CAGGCTCTGA GGAGGGAGAT 
CCACGTACCA AGAGCAGCCT TGCTAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTGGAT 
GTGGTCATCG ACGGGCGCGC GGTGGAGACG CCTCCGATGT CTGCTGTCAA CCTGCTGGAG 
GGGCTGAGCA GGACCGTGGT GTACATGACA TACAGCCAGC TGACCACTCT GGTTGGCTTT 
ATGCGCAACG TGATGTCAAG CGATCAGCTT AAGGAAGATC GGATGGCTTT GGAAAACTTG 
CTGGCAAACT TACCCCAGAA CAAGCCAGGG AAAAGCAGCA GCCTTGAAAT GACTCCATAT 
AACACCCCCC AGCTTTCTCC TGCGACCACT CCAGCCAACA AAAAAAACCG GTTACCGATA 
GGACAGCAAT TGGCAGCCAT TACTGCCTGG GACACCTCTG CCACCAATCT GTCAGCTCAC 
ATAACTCTAG TAACCCCTTT TGCAACTCGT AGCAGAAGTA GATCAAATAT TCTGATGGAT 
CAGCACGGAG ATCACGAAGG ATCCTCCCAG GAAACTATCC CAGAAGTTCA GCCAGAAGAA 
GTGCTGGTGA TTTCTCTGGG GACAGGTCCA CAGATTACTC CAGGAATGAT GTCAGAAAAT 
GAGGTCTTAA ACATGCAGCT TGCAGATGGC GGGCAAGATG TCCCCATTGA CGAAAACAAA 
CTCCATGGTC CTTCCAATCG CTCCAACTCC GTGTCCTCGC TGGATTTGGA AGGGGAGTCG 
GTGTCGGAGC TCGGCGCGGG CCCCTCCGGG AGCAACGGTG TGGAGGCTCT GCAGCTCCTG 
GAGCATGAAC AAGCCACAAC TCAGGATAAT CTGGATGACA AGCTCCGTAA GTTTGAAATC 
CGTGATATGA TGGGTTTGAC TGATGACAGA GATATATCAG AAACTGTGAG TGAAACCTGG 
AGCACAGATG TCTTGGGAAG TGACTTCGAC CCCAACATGG ACGAGGACCG GCTGCAGGAG 
ATCGCAGGTG CAGCTGCAGA GAACATGCTA GGCAGCTTGC TGTGTTTGCC AGGTTCAGGA 
TCCGTGTTGC TTGATCCCTG CACAGGTTCA ACCATATCAG AAACCACAAG TGAGGCGTGG 
AGTGTGGAAG TATTACCGAG TGATTCAGAG GCTCCAGACT TAAAACAGGA GGAAAGGCTC 
CAAGAACTGG AGAGCTGTTC TGGGCTGGGC AGCACGTCTG ATGACACAGA TGTGAGGGAG 
GTCAGCTCTC GACCCAGCAC TCCAGGACTC AGCGTTGTGT CAGGTATTAG TGCAACATCT 
GAAGATATTC CTAACAAGAT TGAGGATCTC AGATCTGAAT GTAGCTCTGA CTTTGGGGGA 
AAAGATTCTG TGACAAGTCC TGATATGGAT GAAACAGCCC ATGGAGCCAG TCAATTGACA 
TCTCCACCTT CTCAGACAGA TTCTTTGCTT GCACTGTTTG ACCCTCTGTC CTCAAATGAG 
GGTGTGTCAG CTGTAGTAAG GCCTAAAGTG CACTATGCAA GACCCTCTCA CCCACCTCCG 
GATCCACCCA TCCTGGAAGG AGCTGTGGGA GGTAACGAGG CCCGACTGCC AAACTTTGGG 
TCTCACGCTC TGATTCCAAC CGATCTGGAA GCCTTCAAGC AGAGACATTC CTACCCGGAG 
AGGCTGGTCC GCAGCCGGAG CTCAGACATA GTGTCGTCGG TTCGGAGGCC CATGAGTGAT 
CCCGGGTGGA ACAGGCGTCC TGGGAACGAG GACAGGGAGC TGCCCATGGC CACCAGCAGC 
GCTGGAGCAG CTGTGCTGGC AGCCACGTCT CAGTCATCGT CTTCATCTCC CAGTAAGGAC 
TCCTCCAGGG GAGAGATTGA GGAACGAAAA GACAGCGATG ATGAGAAGTC TGACAGGAAC 
AAGCCCTGGT GGAGGAAACG TTTTGCGTCT GCCATGCCCA AAGCTCCTAT TCCATTTAGA 
AAGAAAGAAA AACAAGAGAA AGACAAAGAT GACATGGTGC CTGACAGATA CGCAACACTT 
CAAGATGATC CCAGCCCAAG GCTCAGTGCA CAGGCACAAG CTGCTGAGGA CATTCTGGAC 
AAATATAGGA ATGCCATCAA GAGAACCAGT CCCAGTGAGG GAGCCATAGT GAACTATGAC 
AGTGCAGAGG CTCTTGGGGA TGGTGAGAGC ATGCACGACT CCCCGCGGGA CGAGGCCTTG 
CAGAACATGT CTGCAGACGA TCTCCCAGAC TCTGCAAGTC AAGTAGCACA GCCACAAAGT 
TCTGCTTTCT CCTATAGGGA TGCAAAGAAG AAATTGAGAT TGGCTCTTTG TTCAGCAGAT 
TCTGTTGCCT TCCCGATGTT GACCCACTCA ACACGGAATG GTCTCCCAGA CCACACAGAC 
CCCGAAGATA ATGAAATTGT GTGCTTCTTG AAAGTTCAGC TGGCTGAAGC CATCAACCTC 
CAGGACAAGA ACCTGATGGC ACAGCTGCAG GAGACGATGC GCTGCGTGAG CCGCTTCGAC 
AACAGGACCT GTCGGAAGCT GCTGGCCTCC ATTGCAGAGG ACTATAGGAA AAGAGCTCCA 
TATATTGCTT ATTTGACTCG CTGTCGCCAA GGCCTGCAGA CGACACAGGC GCACCTGGAA 
AGGCTCTTGC AGAGAGTTCT ACGAGATAAA GAAGTGGCCA ACAGATACTT CACTACAGTA 
TGTGTGAGGT TACTGCTGGA GAGCAAAGAA AAGAAAATAA GGGAATTTAT TCAAGATTTC 
CAGAAGCTCA CAGCAGCAGA CGATAAAACA GCCCAAGTTG AGGATTTCCT TCAGTTCCTG 
TACGGGGCTA TGGCTCAGGA TGCCATATGG CAGAATGCCA GTGAGGAACA GCTTCAGGAT 
GCACAATTAG CCATCGAGCG CAGTGTGATG AATCGCATTT TCAAACTTGC CTTCTACCCT 
AATCAGGATG GAGATATTCT GCGTGACCAG GTCCTTCACG AGCACATACA GAGGTTATCC 
AAAGTAGTGA CTGCAAACCA CAAGGCCCTT CAGATACCTG AGGTGTATCT CCGGGAGGCA 
CCGTGGCCGT CGGCACAGTC CGAGATCCGC ACGATAAGTG CTTACAAAAC CCCCCGGGAC 
AAGGTGCAGT GTATCCTGAG GATGTGCTCC ACCATCATGA ACCTGCTCAG CCTGGCCAAC 
GAGGATTCAG TACCTGGGGC AGATGATTTT GTTCCTGTTT TGGTTTTTGT CCTGATAAAG 
GCAAACCCCC CTTGCCTGCT GTCCACTGTG CAGTACATCA GTAGTTTCTA TGCCAACTGC 
TTGTCTGGAG AGGAGTCGTA CTGGTGGATG CAGTTCACGG CAGCCGTGGA GTTCATCAAA 
ACCATCGATG ATCGCAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPVD1 ( XM_017810050.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017665539.1 Lepidothrix coronata GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OLe54933
Clone ID Related Accession (Same CDS sequence)	XM_017810050.1
Accession Version	XM_017810050.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690208.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGC TGGACATCCA CACGCTGGCC CACCACCTGA AGCAGGAGCG GCTCTACGTG 
ACGGCGGAGA AGCAGCTGAT CCAGCGGCTC AACGCCGACG TGCTGAAGAC GGCGGAGAAG 
CTCTACCGGA CAGCCTGGAT CTCCAAACAG CAGCGCATCA ACCTGGACAG GCTCATCATC 
ACCAGTGCTG AAGCTTCTCC TGCTGAATGT TGCCAACATG CAAAAATCTT GGAGGACACA 
CAGTTTGTGG ATGGATATAA GCAGCTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCCTG 
AACAGACTGA GAGAGAACCC GAGGCTTATT GCATCCTGCC TGGTTGCTGG AGAGAAGCTC 
AACCAGGACA ACACTCAGAG TGTCATTCAC ACAGTCTTTA CCTCCATTTA TGGCAACTGC 
ATCATGCAGG AGGATGAGAG TTACCTGCTC CAGGTCCTCC GTTACCTGAT TGAGTTTGAA 
CTCAAGGAGA GCGACAACCC CCGGCGGCTG CTCCGACGAG GCACCTGTGC CTTCAGCATC 
TTGTTCAAAC TCTTCTCTGA AGGACTCTTC TCTGCAAAAC TTTTTCTTAC TGCAACCTTA 
CATGAGCCAA TCATGCAGCT CCTGGTTGAA GATGAAGACC ACCTGGAAAC CGATGCAAAC 
AAGTTAATTG AGAGATTCTC CCCAGTACAG CAGGAAAAGT TATTTGGAGA GAAAGGCACA 
GAAAAGTTCA AGCAAAGAGT CCAAGAGATG GTTGACTCCA ACGAGGCCAA GCTGGTGACT 
TTGGTCAACA AATTCATTGG CTATCTCAAA CAAAACACTT ACTGTTTTCC TCACAGCTTG 
AGGTGGATTG TGTCGCAGAT GTACAAAACG CTGTCGTGTG TGGACAGGCT GGAGGTTGGG 
GAGGTCAGAG CCATGTGCAC AGATCTTCTT CTGGCCTGTT TCATCTGCCC TGCAATCGTT 
AACCCAGAGC AATATGGGAT CATTTCTGAT GCTCCTATAA ATGAGGTGGC AAGATTTAAT 
CTGATGCAGG TTGGGAGGCT CCTGCAGCAG CTGGCAATGA CAGGCTCTGA GGAGGGAGAT 
CCACGTACCA AGAGCAGCCT TGCTAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTGGAT 
GTGGTCATCG ACGGGCGCGC GGTGGAGACG CCTCCGATGT CTGCTGTCAA CCTGCTGGAG 
GGGCTGAGCA GGACCGTGGT GTACATGACA TACAGCCAGC TGACCACTCT GGTTGGCTTT 
ATGCGCAACG TGATGTCAAG CGATCAGCTT AAGGAAGATC GGATGGCTTT GGAAAACTTG 
CTGGCAAACT TACCCCAGAA CAAGCCAGGG AAAAGCAGCA GCCTTGAAAT GACTCCATAT 
AACACCCCCC AGCTTTCTCC TGCGACCACT CCAGCCAACA AAAAAAACCG GTTACCGATA 
GGACAGCAAT TGGCAGCCAT TACTGCCTGG GACACCTCTG CCACCAATCT GTCAGCTCAC 
ATAACTCTAG TAACCCCTTT TGCAACTCGT AGCAGAAGTA GATCAAATAT TCTGATGGAT 
CAGCACGGAG ATCACGAAGG ATCCTCCCAG GAAACTATCC CAGAAGTTCA GCCAGAAGAA 
GTGCTGGTGA TTTCTCTGGG GACAGGTCCA CAGATTACTC CAGGAATGAT GTCAGAAAAT 
GAGGTCTTAA ACATGCAGCT TGCAGATGGC GGGCAAGATG TCCCCATTGA CGAAAACAAA 
CTCCATGGTA AACCTGATAA AACCTTGCGC TTTTCCCTCT GCAGTGATAA TCTGGAAGGA 
ATATCTGAAG GTCCTTCCAA TCGCTCCAAC TCCGTGTCCT CGCTGGATTT GGAAGGGGAG 
TCGGTGTCGG AGCTCGGCGC GGGCCCCTCC GGGAGCAACG GTGTGGAGGC TCTGCAGCTC 
CTGGAGCATG AACAAGCCAC AACTCAGGAT AATCTGGATG ACAAGCTCCG TAAGTTTGAA 
ATCCGTGATA TGATGGGTTT GACTGATGAC AGAGATATAT CAGAAACTGT GAGTGAAACC 
TGGAGCACAG ATGTCTTGGG AAGTGACTTC GACCCCAACA TGGACGAGGA CCGGCTGCAG 
GAGATCGCAG GTTCAACCAT ATCAGAAACC ACAAGTGAGG CGTGGAGTGT GGAAGTATTA 
CCGAGTGATT CAGAGGCTCC AGACTTAAAA CAGGAGGAAA GGCTCCAAGA ACTGGAGAGC 
TGTTCTGGGC TGGGCAGCAC GTCTGATGAC ACAGATGTGA GGGAGGTCAG CTCTCGACCC 
AGCACTCCAG GACTCAGCGT TGTGTCAGGT ATTAGTGCAA CATCTGAAGA TATTCCTAAC 
AAGATTGAGG ATCTCAGATC TGAATGTAGC TCTGACTTTG GGGGAAAAGA TTCTGTGACA 
AGTCCTGATA TGGATGAAAC AGCCCATGGA GCCAGTCAAT TGACATCTCC ACCTTCTCAG 
ACAGATTCTT TGCTTGCACT GTTTGACCCT CTGTCCTCAA ATGAGGGTGT GTCAGCTGTA 
GTAAGGCCTA AAGTGCACTA TGCAAGACCC TCTCACCCAC CTCCGGATCC ACCCATCCTG 
GAAGGAGCTG TGGGAGGTAA CGAGGCCCGA CTGCCAAACT TTGGGTCTCA CGCTCTGATT 
CCAACCGATC TGGAAGCCTT CAAGCAGAGA CATTCCTACC CGGAGAGGCT GGTCCGCAGC 
CGGAGCTCAG ACATAGTGTC GTCGGTTCGG AGGCCCATGA GTGATCCCGG GTGGAACAGG 
CGTCCTGGGA ACGAGGACAG GGAGCTGCCC ATGGCCACCA GCAGCGCTGG AGCAGCTGTG 
CTGGCAGCCA CGTCTCAGTC ATCGTCTTCA TCTCCCAGTA AGGACTCCTC CAGGGGAGAG 
ATTGAGGAAC GAAAAGACAG CGATGATGAG AAGTCTGACA GGAACAAGCC CTGGTGGAGG 
AAACGTTTTG CGTCTGCCAT GCCCAAAGCT CCTATTCCAT TTAGAAAGAA AGAAAAACAA 
GAGAAAGACA AAGATGACAT GGTGCCTGAC AGATACGCAA CACTTCAAGA TGATCCCAGC 
CCAAGGCTCA GTGCACAGGC ACAAGCTGCT GAGGACATTC TGGACAAATA TAGGAATGCC 
ATCAAGAGAA CCAGTCCCAG TGAGGGAGCC ATAGTGAACT ATGACAGTGC AGAGGCTCTT 
GGGGATGGTG AGAGCATGCA CGACTCCCCG CGGGACGAGG CCTTGCAGAA CATGTCTGCA 
GACGATCTCC CAGACTCTGC AAGTCAAGTA GCACAGCCAC AAAGTTCTGC TTTCTCCTAT 
AGGGATGCAA AGAAGAAATT GAGATTGGCT CTTTGTTCAG CAGATTCTGT TGCCTTCCCG 
ATGTTGACCC ACTCAACACG GAATGGTCTC CCAGACCACA CAGACCCCGA AGATAATGAA 
ATTGTGTGCT TCTTGAAAGT TCAGCTGGCT GAAGCCATCA ACCTCCAGGA CAAGAACCTG 
ATGGCACAGC TGCAGGAGAC GATGCGCTGC GTGAGCCGCT TCGACAACAG GACCTGTCGG 
AAGCTGCTGG CCTCCATTGC AGAGGACTAT AGGAAAAGAG CTCCATATAT TGCTTATTTG 
ACTCGCTGTC GCCAAGGCCT GCAGACGACA CAGGCGCACC TGGAAAGGCT CTTGCAGAGA 
GTTCTACGAG ATAAAGAAGT GGCCAACAGA TACTTCACTA CAGTATGTGT GAGGTTACTG 
CTGGAGAGCA AAGAAAAGAA AATAAGGGAA TTTATTCAAG ATTTCCAGAA GCTCACAGCA 
GCAGACGATA AAACAGCCCA AGTTGAGGAT TTCCTTCAGT TCCTGTACGG GGCTATGGCT 
CAGGATGCCA TATGGCAGAA TGCCAGTGAG GAACAGCTTC AGGATGCACA ATTAGCCATC 
GAGCGCAGTG TGATGAATCG CATTTTCAAA CTTGCCTTCT ACCCTAATCA GGATGGAGAT 
ATTCTGCGTG ACCAGGTCCT TCACGAGCAC ATACAGAGGT TATCCAAAGT AGTGACTGCA 
AACCACAAGG CCCTTCAGAT ACCTGAGGTG TATCTCCGGG AGGCACCGTG GCCGTCGGCA 
CAGTCCGAGA TCCGCACGAT AAGTGCTTAC AAAACCCCCC GGGACAAGGT GCAGTGTATC 
CTGAGGATGT GCTCCACCAT CATGAACCTG CTCAGCCTGG CCAACGAGGA TTCAGTACCT 
GGGGCAGATG ATTTTGTTCC TGTTTTGGTT TTTGTCCTGA TAAAGGCAAA CCCCCCTTGC 
CTGCTGTCCA CTGTGCAGTA CATCAGTAGT TTCTATGCCA ACTGCTTGTC TGGAGAGGAG 
TCGTACTGGT GGATGCAGTT CACGGCAGCC GTGGAGTTCA TCAAAACCAT CGATGATCGC 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017665539.1
CDS	162..4547
Translation	MVKLDIHTLAHHLKQERLYVTAEKQLIQRLNADVLKTAEKLYRTAWISKQQRINLDRLIITSAEASPAECCQHAKILEDTQFVDGYKQLGFQETAYGEFLNRLRENPRLIASCLVAGEKLNQDNTQSVIHTVFTSIYGNCIMQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDANKLIERFSPVQQEKLFGEKGTEKFKQRVQEMVDSNEAKLVTLVNKFIGYLKQNTYCFPHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAIVNPEQYGIISDAPINEVARFNLMQVGRLLQQLAMTGSEEGDPRTKSSLAKFDKSCVAAFLDVVIDGRAVETPPMSAVNLLEGLSRTVVYMTYSQLTTLVGFMRNVMSSDQLKEDRMALENLLANLPQNKPGKSSSLEMTPYNTPQLSPATTPANKKNRLPIGQQLAAITAWDTSATNLSAHITLVTPFATRSRSRSNILMDQHGDHEGSSQETIPEVQPEEVLVISLGTGPQITPGMMSENEVLNMQLADGGQDVPIDENKLHGKPDKTLRFSLCSDNLEGISEGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNMDEDRLQEIAGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDETAHGASQLTSPPSQTDSLLALFDPLSSNEGVSAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHALIPTDLEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPGWNRRPGNEDRELPMATSSAGAAVLAATSQSSSSSPSKDSSRGEIEERKDSDDEKSDRNKPWWRKRFASAMPKAPIPFRKKEKQEKDKDDMVPDRYATLQDDPSPRLSAQAQAAEDILDKYRNAIKRTSPSEGAIVNYDSAEALGDGESMHDSPRDEALQNMSADDLPDSASQVAQPQSSAFSYRDAKKKLRLALCSADSVAFPMLTHSTRNGLPDHTDPEDNEIVCFLKVQLAEAINLQDKNLMAQLQETMRCVSRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGAMAQDAIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHKALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYANCLSGEESYWWMQFTAAVEFIKTIDDRK

Target ORF information:

RefSeq Version	XM_017810050.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017810050.1

ORF Insert Sequence:

ATGGTGAAGC TGGACATCCA CACGCTGGCC CACCACCTGA AGCAGGAGCG GCTCTACGTG 
ACGGCGGAGA AGCAGCTGAT CCAGCGGCTC AACGCCGACG TGCTGAAGAC GGCGGAGAAG 
CTCTACCGGA CAGCCTGGAT CTCCAAACAG CAGCGCATCA ACCTGGACAG GCTCATCATC 
ACCAGTGCTG AAGCTTCTCC TGCTGAATGT TGCCAACATG CAAAAATCTT GGAGGACACA 
CAGTTTGTGG ATGGATATAA GCAGCTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCCTG 
AACAGACTGA GAGAGAACCC GAGGCTTATT GCATCCTGCC TGGTTGCTGG AGAGAAGCTC 
AACCAGGACA ACACTCAGAG TGTCATTCAC ACAGTCTTTA CCTCCATTTA TGGCAACTGC 
ATCATGCAGG AGGATGAGAG TTACCTGCTC CAGGTCCTCC GTTACCTGAT TGAGTTTGAA 
CTCAAGGAGA GCGACAACCC CCGGCGGCTG CTCCGACGAG GCACCTGTGC CTTCAGCATC 
TTGTTCAAAC TCTTCTCTGA AGGACTCTTC TCTGCAAAAC TTTTTCTTAC TGCAACCTTA 
CATGAGCCAA TCATGCAGCT CCTGGTTGAA GATGAAGACC ACCTGGAAAC CGATGCAAAC 
AAGTTAATTG AGAGATTCTC CCCAGTACAG CAGGAAAAGT TATTTGGAGA GAAAGGCACA 
GAAAAGTTCA AGCAAAGAGT CCAAGAGATG GTTGACTCCA ACGAGGCCAA GCTGGTGACT 
TTGGTCAACA AATTCATTGG CTATCTCAAA CAAAACACTT ACTGTTTTCC TCACAGCTTG 
AGGTGGATTG TGTCGCAGAT GTACAAAACG CTGTCGTGTG TGGACAGGCT GGAGGTTGGG 
GAGGTCAGAG CCATGTGCAC AGATCTTCTT CTGGCCTGTT TCATCTGCCC TGCAATCGTT 
AACCCAGAGC AATATGGGAT CATTTCTGAT GCTCCTATAA ATGAGGTGGC AAGATTTAAT 
CTGATGCAGG TTGGGAGGCT CCTGCAGCAG CTGGCAATGA CAGGCTCTGA GGAGGGAGAT 
CCACGTACCA AGAGCAGCCT TGCTAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTGGAT 
GTGGTCATCG ACGGGCGCGC GGTGGAGACG CCTCCGATGT CTGCTGTCAA CCTGCTGGAG 
GGGCTGAGCA GGACCGTGGT GTACATGACA TACAGCCAGC TGACCACTCT GGTTGGCTTT 
ATGCGCAACG TGATGTCAAG CGATCAGCTT AAGGAAGATC GGATGGCTTT GGAAAACTTG 
CTGGCAAACT TACCCCAGAA CAAGCCAGGG AAAAGCAGCA GCCTTGAAAT GACTCCATAT 
AACACCCCCC AGCTTTCTCC TGCGACCACT CCAGCCAACA AAAAAAACCG GTTACCGATA 
GGACAGCAAT TGGCAGCCAT TACTGCCTGG GACACCTCTG CCACCAATCT GTCAGCTCAC 
ATAACTCTAG TAACCCCTTT TGCAACTCGT AGCAGAAGTA GATCAAATAT TCTGATGGAT 
CAGCACGGAG ATCACGAAGG ATCCTCCCAG GAAACTATCC CAGAAGTTCA GCCAGAAGAA 
GTGCTGGTGA TTTCTCTGGG GACAGGTCCA CAGATTACTC CAGGAATGAT GTCAGAAAAT 
GAGGTCTTAA ACATGCAGCT TGCAGATGGC GGGCAAGATG TCCCCATTGA CGAAAACAAA 
CTCCATGGTA AACCTGATAA AACCTTGCGC TTTTCCCTCT GCAGTGATAA TCTGGAAGGA 
ATATCTGAAG GTCCTTCCAA TCGCTCCAAC TCCGTGTCCT CGCTGGATTT GGAAGGGGAG 
TCGGTGTCGG AGCTCGGCGC GGGCCCCTCC GGGAGCAACG GTGTGGAGGC TCTGCAGCTC 
CTGGAGCATG AACAAGCCAC AACTCAGGAT AATCTGGATG ACAAGCTCCG TAAGTTTGAA 
ATCCGTGATA TGATGGGTTT GACTGATGAC AGAGATATAT CAGAAACTGT GAGTGAAACC 
TGGAGCACAG ATGTCTTGGG AAGTGACTTC GACCCCAACA TGGACGAGGA CCGGCTGCAG 
GAGATCGCAG GTTCAACCAT ATCAGAAACC ACAAGTGAGG CGTGGAGTGT GGAAGTATTA 
CCGAGTGATT CAGAGGCTCC AGACTTAAAA CAGGAGGAAA GGCTCCAAGA ACTGGAGAGC 
TGTTCTGGGC TGGGCAGCAC GTCTGATGAC ACAGATGTGA GGGAGGTCAG CTCTCGACCC 
AGCACTCCAG GACTCAGCGT TGTGTCAGGT ATTAGTGCAA CATCTGAAGA TATTCCTAAC 
AAGATTGAGG ATCTCAGATC TGAATGTAGC TCTGACTTTG GGGGAAAAGA TTCTGTGACA 
AGTCCTGATA TGGATGAAAC AGCCCATGGA GCCAGTCAAT TGACATCTCC ACCTTCTCAG 
ACAGATTCTT TGCTTGCACT GTTTGACCCT CTGTCCTCAA ATGAGGGTGT GTCAGCTGTA 
GTAAGGCCTA AAGTGCACTA TGCAAGACCC TCTCACCCAC CTCCGGATCC ACCCATCCTG 
GAAGGAGCTG TGGGAGGTAA CGAGGCCCGA CTGCCAAACT TTGGGTCTCA CGCTCTGATT 
CCAACCGATC TGGAAGCCTT CAAGCAGAGA CATTCCTACC CGGAGAGGCT GGTCCGCAGC 
CGGAGCTCAG ACATAGTGTC GTCGGTTCGG AGGCCCATGA GTGATCCCGG GTGGAACAGG 
CGTCCTGGGA ACGAGGACAG GGAGCTGCCC ATGGCCACCA GCAGCGCTGG AGCAGCTGTG 
CTGGCAGCCA CGTCTCAGTC ATCGTCTTCA TCTCCCAGTA AGGACTCCTC CAGGGGAGAG 
ATTGAGGAAC GAAAAGACAG CGATGATGAG AAGTCTGACA GGAACAAGCC CTGGTGGAGG 
AAACGTTTTG CGTCTGCCAT GCCCAAAGCT CCTATTCCAT TTAGAAAGAA AGAAAAACAA 
GAGAAAGACA AAGATGACAT GGTGCCTGAC AGATACGCAA CACTTCAAGA TGATCCCAGC 
CCAAGGCTCA GTGCACAGGC ACAAGCTGCT GAGGACATTC TGGACAAATA TAGGAATGCC 
ATCAAGAGAA CCAGTCCCAG TGAGGGAGCC ATAGTGAACT ATGACAGTGC AGAGGCTCTT 
GGGGATGGTG AGAGCATGCA CGACTCCCCG CGGGACGAGG CCTTGCAGAA CATGTCTGCA 
GACGATCTCC CAGACTCTGC AAGTCAAGTA GCACAGCCAC AAAGTTCTGC TTTCTCCTAT 
AGGGATGCAA AGAAGAAATT GAGATTGGCT CTTTGTTCAG CAGATTCTGT TGCCTTCCCG 
ATGTTGACCC ACTCAACACG GAATGGTCTC CCAGACCACA CAGACCCCGA AGATAATGAA 
ATTGTGTGCT TCTTGAAAGT TCAGCTGGCT GAAGCCATCA ACCTCCAGGA CAAGAACCTG 
ATGGCACAGC TGCAGGAGAC GATGCGCTGC GTGAGCCGCT TCGACAACAG GACCTGTCGG 
AAGCTGCTGG CCTCCATTGC AGAGGACTAT AGGAAAAGAG CTCCATATAT TGCTTATTTG 
ACTCGCTGTC GCCAAGGCCT GCAGACGACA CAGGCGCACC TGGAAAGGCT CTTGCAGAGA 
GTTCTACGAG ATAAAGAAGT GGCCAACAGA TACTTCACTA CAGTATGTGT GAGGTTACTG 
CTGGAGAGCA AAGAAAAGAA AATAAGGGAA TTTATTCAAG ATTTCCAGAA GCTCACAGCA 
GCAGACGATA AAACAGCCCA AGTTGAGGAT TTCCTTCAGT TCCTGTACGG GGCTATGGCT 
CAGGATGCCA TATGGCAGAA TGCCAGTGAG GAACAGCTTC AGGATGCACA ATTAGCCATC 
GAGCGCAGTG TGATGAATCG CATTTTCAAA CTTGCCTTCT ACCCTAATCA GGATGGAGAT 
ATTCTGCGTG ACCAGGTCCT TCACGAGCAC ATACAGAGGT TATCCAAAGT AGTGACTGCA 
AACCACAAGG CCCTTCAGAT ACCTGAGGTG TATCTCCGGG AGGCACCGTG GCCGTCGGCA 
CAGTCCGAGA TCCGCACGAT AAGTGCTTAC AAAACCCCCC GGGACAAGGT GCAGTGTATC 
CTGAGGATGT GCTCCACCAT CATGAACCTG CTCAGCCTGG CCAACGAGGA TTCAGTACCT 
GGGGCAGATG ATTTTGTTCC TGTTTTGGTT TTTGTCCTGA TAAAGGCAAA CCCCCCTTGC 
CTGCTGTCCA CTGTGCAGTA CATCAGTAGT TTCTATGCCA ACTGCTTGTC TGGAGAGGAG 
TCGTACTGGT GGATGCAGTT CACGGCAGCC GTGGAGTTCA TCAAAACCAT CGATGATCGC 
AAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPVD1 ( XM_017810051.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017665540.1 Lepidothrix coronata GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OLe54934
Clone ID Related Accession (Same CDS sequence)	XM_017810051.1
Accession Version	XM_017810051.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4383bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690208.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGC TGGACATCCA CACGCTGGCC CACCACCTGA AGCAGGAGCG GCTCTACGTG 
ACGGCGGAGA AGCAGCTGAT CCAGCGGCTC AACGCCGACG TGCTGAAGAC GGCGGAGAAG 
CTCTACCGGA CAGCCTGGAT CTCCAAACAG CAGCGCATCA ACCTGGACAG GCTCATCATC 
ACCAGTGCTG AAGCTTCTCC TGCTGAATGT TGCCAACATG CAAAAATCTT GGAGGACACA 
CAGTTTGTGG ATGGATATAA GCAGCTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCCTG 
AACAGACTGA GAGAGAACCC GAGGCTTATT GCATCCTGCC TGGTTGCTGG AGAGAAGCTC 
AACCAGGACA ACACTCAGAG TGTCATTCAC ACAGTCTTTA CCTCCATTTA TGGCAACTGC 
ATCATGCAGG AGGATGAGAG TTACCTGCTC CAGGTCCTCC GTTACCTGAT TGAGTTTGAA 
CTCAAGGAGA GCGACAACCC CCGGCGGCTG CTCCGACGAG GCACCTGTGC CTTCAGCATC 
TTGTTCAAAC TCTTCTCTGA AGGACTCTTC TCTGCAAAAC TTTTTCTTAC TGCAACCTTA 
CATGAGCCAA TCATGCAGCT CCTGGTTGAA GATGAAGACC ACCTGGAAAC CGATGCAAAC 
AAGTTAATTG AGAGATTCTC CCCAGTACAG CAGGAAAAGT TATTTGGAGA GAAAGGCACA 
GAAAAGTTCA AGCAAAGAGT CCAAGAGATG GTTGACTCCA ACGAGGCCAA GCTGGTGACT 
TTGGTCAACA AATTCATTGG CTATCTCAAA CAAAACACTT ACTGTTTTCC TCACAGCTTG 
AGGTGGATTG TGTCGCAGAT GTACAAAACG CTGTCGTGTG TGGACAGGCT GGAGGTTGGG 
GAGGTCAGAG CCATGTGCAC AGATCTTCTT CTGGCCTGTT TCATCTGCCC TGCAATCGTT 
AACCCAGAGC AATATGGGAT CATTTCTGAT GCTCCTATAA ATGAGGTGGC AAGATTTAAT 
CTGATGCAGG TTGGGAGGCT CCTGCAGCAG CTGGCAATGA CAGGCTCTGA GGAGGGAGAT 
CCACGTACCA AGAGCAGCCT TGCTAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTGGAT 
GTGGTCATCG ACGGGCGCGC GGTGGAGACG CCTCCGATGT CTGCTGTCAA CCTGCTGGAG 
GGGCTGAGCA GGACCGTGGT GTACATGACA TACAGCCAGC TGACCACTCT GGTTGGCTTT 
ATGCGCAACG TGATGTCAAG CGATCAGCTT AAGGAAGATC GGATGGCTTT GGAAAACTTG 
CTGGCAAACT TACCCCAGAA CAAGCCAGGG AAAAGCAGCA GCCTTGAAAT GACTCCATAT 
AACACCCCCC AGCTTTCTCC TGCGACCACT CCAGCCAACA AAAAAAACCG GTTACCGATA 
GCAACTCGTA GCAGAAGTAG ATCAAATATT CTGATGGATC AGCACGGAGA TCACGAAGGA 
TCCTCCCAGG AAACTATCCC AGAAGTTCAG CCAGAAGAAG TGCTGGTGAT TTCTCTGGGG 
ACAGGTCCAC AGATTACTCC AGGAATGATG TCAGAAAATG AGGTCTTAAA CATGCAGCTT 
GCAGATGGCG GGCAAGATGT CCCCATTGAC GAAAACAAAC TCCATGGTAA ACCTGATAAA 
ACCTTGCGCT TTTCCCTCTG CAGTGATAAT CTGGAAGGAA TATCTGAAGG TCCTTCCAAT 
CGCTCCAACT CCGTGTCCTC GCTGGATTTG GAAGGGGAGT CGGTGTCGGA GCTCGGCGCG 
GGCCCCTCCG GGAGCAACGG TGTGGAGGCT CTGCAGCTCC TGGAGCATGA ACAAGCCACA 
ACTCAGGATA ATCTGGATGA CAAGCTCCGT AAGTTTGAAA TCCGTGATAT GATGGGTTTG 
ACTGATGACA GAGATATATC AGAAACTGTG AGTGAAACCT GGAGCACAGA TGTCTTGGGA 
AGTGACTTCG ACCCCAACAT GGACGAGGAC CGGCTGCAGG AGATCGCAGG TGCAGCTGCA 
GAGAACATGC TAGGCAGCTT GCTGTGTTTG CCAGGTTCAG GATCCGTGTT GCTTGATCCC 
TGCACAGGTT CAACCATATC AGAAACCACA AGTGAGGCGT GGAGTGTGGA AGTATTACCG 
AGTGATTCAG AGGCTCCAGA CTTAAAACAG GAGGAAAGGC TCCAAGAACT GGAGAGCTGT 
TCTGGGCTGG GCAGCACGTC TGATGACACA GATGTGAGGG AGGTCAGCTC TCGACCCAGC 
ACTCCAGGAC TCAGCGTTGT GTCAGGTATT AGTGCAACAT CTGAAGATAT TCCTAACAAG 
ATTGAGGATC TCAGATCTGA ATGTAGCTCT GACTTTGGGG GAAAAGATTC TGTGACAAGT 
CCTGATATGG ATGAAACAGC CCATGGAGCC AGTCAATTGA CATCTCCACC TTCTCAGACA 
GATTCTTTGC TTGCACTGTT TGACCCTCTG TCCTCAAATG AGGGTGTGTC AGCTGTAGTA 
AGGCCTAAAG TGCACTATGC AAGACCCTCT CACCCACCTC CGGATCCACC CATCCTGGAA 
GGAGCTGTGG GAGGTAACGA GGCCCGACTG CCAAACTTTG GGTCTCACGC TCTGATTCCA 
ACCGATCTGG AAGCCTTCAA GCAGAGACAT TCCTACCCGG AGAGGCTGGT CCGCAGCCGG 
AGCTCAGACA TAGTGTCGTC GGTTCGGAGG CCCATGAGTG ATCCCGGGTG GAACAGGCGT 
CCTGGGAACG AGGACAGGGA GCTGCCCATG GCCACCAGCA GCGCTGGAGC AGCTGTGCTG 
GCAGCCACGT CTCAGTCATC GTCTTCATCT CCCAGTAAGG ACTCCTCCAG GGGAGAGATT 
GAGGAACGAA AAGACAGCGA TGATGAGAAG TCTGACAGGA ACAAGCCCTG GTGGAGGAAA 
CGTTTTGCGT CTGCCATGCC CAAAGCTCCT ATTCCATTTA GAAAGAAAGA AAAACAAGAG 
AAAGACAAAG ATGACATGGT GCCTGACAGA TACGCAACAC TTCAAGATGA TCCCAGCCCA 
AGGCTCAGTG CACAGGCACA AGCTGCTGAG GACATTCTGG ACAAATATAG GAATGCCATC 
AAGAGAACCA GTCCCAGTGA GGGAGCCATA GTGAACTATG ACAGTGCAGA GGCTCTTGGG 
GATGGTGAGA GCATGCACGA CTCCCCGCGG GACGAGGCCT TGCAGAACAT GTCTGCAGAC 
GATCTCCCAG ACTCTGCAAG TCAAGTAGCA CAGCCACAAA GTTCTGCTTT CTCCTATAGG 
GATGCAAAGA AGAAATTGAG ATTGGCTCTT TGTTCAGCAG ATTCTGTTGC CTTCCCGATG 
TTGACCCACT CAACACGGAA TGGTCTCCCA GACCACACAG ACCCCGAAGA TAATGAAATT 
GTGTGCTTCT TGAAAGTTCA GCTGGCTGAA GCCATCAACC TCCAGGACAA GAACCTGATG 
GCACAGCTGC AGGAGACGAT GCGCTGCGTG AGCCGCTTCG ACAACAGGAC CTGTCGGAAG 
CTGCTGGCCT CCATTGCAGA GGACTATAGG AAAAGAGCTC CATATATTGC TTATTTGACT 
CGCTGTCGCC AAGGCCTGCA GACGACACAG GCGCACCTGG AAAGGCTCTT GCAGAGAGTT 
CTACGAGATA AAGAAGTGGC CAACAGATAC TTCACTACAG TATGTGTGAG GTTACTGCTG 
GAGAGCAAAG AAAAGAAAAT AAGGGAATTT ATTCAAGATT TCCAGAAGCT CACAGCAGCA 
GACGATAAAA CAGCCCAAGT TGAGGATTTC CTTCAGTTCC TGTACGGGGC TATGGCTCAG 
GATGCCATAT GGCAGAATGC CAGTGAGGAA CAGCTTCAGG ATGCACAATT AGCCATCGAG 
CGCAGTGTGA TGAATCGCAT TTTCAAACTT GCCTTCTACC CTAATCAGGA TGGAGATATT 
CTGCGTGACC AGGTCCTTCA CGAGCACATA CAGAGGTTAT CCAAAGTAGT GACTGCAAAC 
CACAAGGCCC TTCAGATACC TGAGGTGTAT CTCCGGGAGG CACCGTGGCC GTCGGCACAG 
TCCGAGATCC GCACGATAAG TGCTTACAAA ACCCCCCGGG ACAAGGTGCA GTGTATCCTG 
AGGATGTGCT CCACCATCAT GAACCTGCTC AGCCTGGCCA ACGAGGATTC AGTACCTGGG 
GCAGATGATT TTGTTCCTGT TTTGGTTTTT GTCCTGATAA AGGCAAACCC CCCTTGCCTG 
CTGTCCACTG TGCAGTACAT CAGTAGTTTC TATGCCAACT GCTTGTCTGG AGAGGAGTCG 
TACTGGTGGA TGCAGTTCAC GGCAGCCGTG GAGTTCATCA AAACCATCGA TGATCGCAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017665540.1
CDS	162..4544
Translation	MVKLDIHTLAHHLKQERLYVTAEKQLIQRLNADVLKTAEKLYRTAWISKQQRINLDRLIITSAEASPAECCQHAKILEDTQFVDGYKQLGFQETAYGEFLNRLRENPRLIASCLVAGEKLNQDNTQSVIHTVFTSIYGNCIMQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDANKLIERFSPVQQEKLFGEKGTEKFKQRVQEMVDSNEAKLVTLVNKFIGYLKQNTYCFPHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAIVNPEQYGIISDAPINEVARFNLMQVGRLLQQLAMTGSEEGDPRTKSSLAKFDKSCVAAFLDVVIDGRAVETPPMSAVNLLEGLSRTVVYMTYSQLTTLVGFMRNVMSSDQLKEDRMALENLLANLPQNKPGKSSSLEMTPYNTPQLSPATTPANKKNRLPIATRSRSRSNILMDQHGDHEGSSQETIPEVQPEEVLVISLGTGPQITPGMMSENEVLNMQLADGGQDVPIDENKLHGKPDKTLRFSLCSDNLEGISEGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNMDEDRLQEIAGAAAENMLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDETAHGASQLTSPPSQTDSLLALFDPLSSNEGVSAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHALIPTDLEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPGWNRRPGNEDRELPMATSSAGAAVLAATSQSSSSSPSKDSSRGEIEERKDSDDEKSDRNKPWWRKRFASAMPKAPIPFRKKEKQEKDKDDMVPDRYATLQDDPSPRLSAQAQAAEDILDKYRNAIKRTSPSEGAIVNYDSAEALGDGESMHDSPRDEALQNMSADDLPDSASQVAQPQSSAFSYRDAKKKLRLALCSADSVAFPMLTHSTRNGLPDHTDPEDNEIVCFLKVQLAEAINLQDKNLMAQLQETMRCVSRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGAMAQDAIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHKALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYANCLSGEESYWWMQFTAAVEFIKTIDDRK

Target ORF information:

RefSeq Version	XM_017810051.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017810051.1

ORF Insert Sequence:

ATGGTGAAGC TGGACATCCA CACGCTGGCC CACCACCTGA AGCAGGAGCG GCTCTACGTG 
ACGGCGGAGA AGCAGCTGAT CCAGCGGCTC AACGCCGACG TGCTGAAGAC GGCGGAGAAG 
CTCTACCGGA CAGCCTGGAT CTCCAAACAG CAGCGCATCA ACCTGGACAG GCTCATCATC 
ACCAGTGCTG AAGCTTCTCC TGCTGAATGT TGCCAACATG CAAAAATCTT GGAGGACACA 
CAGTTTGTGG ATGGATATAA GCAGCTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCCTG 
AACAGACTGA GAGAGAACCC GAGGCTTATT GCATCCTGCC TGGTTGCTGG AGAGAAGCTC 
AACCAGGACA ACACTCAGAG TGTCATTCAC ACAGTCTTTA CCTCCATTTA TGGCAACTGC 
ATCATGCAGG AGGATGAGAG TTACCTGCTC CAGGTCCTCC GTTACCTGAT TGAGTTTGAA 
CTCAAGGAGA GCGACAACCC CCGGCGGCTG CTCCGACGAG GCACCTGTGC CTTCAGCATC 
TTGTTCAAAC TCTTCTCTGA AGGACTCTTC TCTGCAAAAC TTTTTCTTAC TGCAACCTTA 
CATGAGCCAA TCATGCAGCT CCTGGTTGAA GATGAAGACC ACCTGGAAAC CGATGCAAAC 
AAGTTAATTG AGAGATTCTC CCCAGTACAG CAGGAAAAGT TATTTGGAGA GAAAGGCACA 
GAAAAGTTCA AGCAAAGAGT CCAAGAGATG GTTGACTCCA ACGAGGCCAA GCTGGTGACT 
TTGGTCAACA AATTCATTGG CTATCTCAAA CAAAACACTT ACTGTTTTCC TCACAGCTTG 
AGGTGGATTG TGTCGCAGAT GTACAAAACG CTGTCGTGTG TGGACAGGCT GGAGGTTGGG 
GAGGTCAGAG CCATGTGCAC AGATCTTCTT CTGGCCTGTT TCATCTGCCC TGCAATCGTT 
AACCCAGAGC AATATGGGAT CATTTCTGAT GCTCCTATAA ATGAGGTGGC AAGATTTAAT 
CTGATGCAGG TTGGGAGGCT CCTGCAGCAG CTGGCAATGA CAGGCTCTGA GGAGGGAGAT 
CCACGTACCA AGAGCAGCCT TGCTAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTGGAT 
GTGGTCATCG ACGGGCGCGC GGTGGAGACG CCTCCGATGT CTGCTGTCAA CCTGCTGGAG 
GGGCTGAGCA GGACCGTGGT GTACATGACA TACAGCCAGC TGACCACTCT GGTTGGCTTT 
ATGCGCAACG TGATGTCAAG CGATCAGCTT AAGGAAGATC GGATGGCTTT GGAAAACTTG 
CTGGCAAACT TACCCCAGAA CAAGCCAGGG AAAAGCAGCA GCCTTGAAAT GACTCCATAT 
AACACCCCCC AGCTTTCTCC TGCGACCACT CCAGCCAACA AAAAAAACCG GTTACCGATA 
GCAACTCGTA GCAGAAGTAG ATCAAATATT CTGATGGATC AGCACGGAGA TCACGAAGGA 
TCCTCCCAGG AAACTATCCC AGAAGTTCAG CCAGAAGAAG TGCTGGTGAT TTCTCTGGGG 
ACAGGTCCAC AGATTACTCC AGGAATGATG TCAGAAAATG AGGTCTTAAA CATGCAGCTT 
GCAGATGGCG GGCAAGATGT CCCCATTGAC GAAAACAAAC TCCATGGTAA ACCTGATAAA 
ACCTTGCGCT TTTCCCTCTG CAGTGATAAT CTGGAAGGAA TATCTGAAGG TCCTTCCAAT 
CGCTCCAACT CCGTGTCCTC GCTGGATTTG GAAGGGGAGT CGGTGTCGGA GCTCGGCGCG 
GGCCCCTCCG GGAGCAACGG TGTGGAGGCT CTGCAGCTCC TGGAGCATGA ACAAGCCACA 
ACTCAGGATA ATCTGGATGA CAAGCTCCGT AAGTTTGAAA TCCGTGATAT GATGGGTTTG 
ACTGATGACA GAGATATATC AGAAACTGTG AGTGAAACCT GGAGCACAGA TGTCTTGGGA 
AGTGACTTCG ACCCCAACAT GGACGAGGAC CGGCTGCAGG AGATCGCAGG TGCAGCTGCA 
GAGAACATGC TAGGCAGCTT GCTGTGTTTG CCAGGTTCAG GATCCGTGTT GCTTGATCCC 
TGCACAGGTT CAACCATATC AGAAACCACA AGTGAGGCGT GGAGTGTGGA AGTATTACCG 
AGTGATTCAG AGGCTCCAGA CTTAAAACAG GAGGAAAGGC TCCAAGAACT GGAGAGCTGT 
TCTGGGCTGG GCAGCACGTC TGATGACACA GATGTGAGGG AGGTCAGCTC TCGACCCAGC 
ACTCCAGGAC TCAGCGTTGT GTCAGGTATT AGTGCAACAT CTGAAGATAT TCCTAACAAG 
ATTGAGGATC TCAGATCTGA ATGTAGCTCT GACTTTGGGG GAAAAGATTC TGTGACAAGT 
CCTGATATGG ATGAAACAGC CCATGGAGCC AGTCAATTGA CATCTCCACC TTCTCAGACA 
GATTCTTTGC TTGCACTGTT TGACCCTCTG TCCTCAAATG AGGGTGTGTC AGCTGTAGTA 
AGGCCTAAAG TGCACTATGC AAGACCCTCT CACCCACCTC CGGATCCACC CATCCTGGAA 
GGAGCTGTGG GAGGTAACGA GGCCCGACTG CCAAACTTTG GGTCTCACGC TCTGATTCCA 
ACCGATCTGG AAGCCTTCAA GCAGAGACAT TCCTACCCGG AGAGGCTGGT CCGCAGCCGG 
AGCTCAGACA TAGTGTCGTC GGTTCGGAGG CCCATGAGTG ATCCCGGGTG GAACAGGCGT 
CCTGGGAACG AGGACAGGGA GCTGCCCATG GCCACCAGCA GCGCTGGAGC AGCTGTGCTG 
GCAGCCACGT CTCAGTCATC GTCTTCATCT CCCAGTAAGG ACTCCTCCAG GGGAGAGATT 
GAGGAACGAA AAGACAGCGA TGATGAGAAG TCTGACAGGA ACAAGCCCTG GTGGAGGAAA 
CGTTTTGCGT CTGCCATGCC CAAAGCTCCT ATTCCATTTA GAAAGAAAGA AAAACAAGAG 
AAAGACAAAG ATGACATGGT GCCTGACAGA TACGCAACAC TTCAAGATGA TCCCAGCCCA 
AGGCTCAGTG CACAGGCACA AGCTGCTGAG GACATTCTGG ACAAATATAG GAATGCCATC 
AAGAGAACCA GTCCCAGTGA GGGAGCCATA GTGAACTATG ACAGTGCAGA GGCTCTTGGG 
GATGGTGAGA GCATGCACGA CTCCCCGCGG GACGAGGCCT TGCAGAACAT GTCTGCAGAC 
GATCTCCCAG ACTCTGCAAG TCAAGTAGCA CAGCCACAAA GTTCTGCTTT CTCCTATAGG 
GATGCAAAGA AGAAATTGAG ATTGGCTCTT TGTTCAGCAG ATTCTGTTGC CTTCCCGATG 
TTGACCCACT CAACACGGAA TGGTCTCCCA GACCACACAG ACCCCGAAGA TAATGAAATT 
GTGTGCTTCT TGAAAGTTCA GCTGGCTGAA GCCATCAACC TCCAGGACAA GAACCTGATG 
GCACAGCTGC AGGAGACGAT GCGCTGCGTG AGCCGCTTCG ACAACAGGAC CTGTCGGAAG 
CTGCTGGCCT CCATTGCAGA GGACTATAGG AAAAGAGCTC CATATATTGC TTATTTGACT 
CGCTGTCGCC AAGGCCTGCA GACGACACAG GCGCACCTGG AAAGGCTCTT GCAGAGAGTT 
CTACGAGATA AAGAAGTGGC CAACAGATAC TTCACTACAG TATGTGTGAG GTTACTGCTG 
GAGAGCAAAG AAAAGAAAAT AAGGGAATTT ATTCAAGATT TCCAGAAGCT CACAGCAGCA 
GACGATAAAA CAGCCCAAGT TGAGGATTTC CTTCAGTTCC TGTACGGGGC TATGGCTCAG 
GATGCCATAT GGCAGAATGC CAGTGAGGAA CAGCTTCAGG ATGCACAATT AGCCATCGAG 
CGCAGTGTGA TGAATCGCAT TTTCAAACTT GCCTTCTACC CTAATCAGGA TGGAGATATT 
CTGCGTGACC AGGTCCTTCA CGAGCACATA CAGAGGTTAT CCAAAGTAGT GACTGCAAAC 
CACAAGGCCC TTCAGATACC TGAGGTGTAT CTCCGGGAGG CACCGTGGCC GTCGGCACAG 
TCCGAGATCC GCACGATAAG TGCTTACAAA ACCCCCCGGG ACAAGGTGCA GTGTATCCTG 
AGGATGTGCT CCACCATCAT GAACCTGCTC AGCCTGGCCA ACGAGGATTC AGTACCTGGG 
GCAGATGATT TTGTTCCTGT TTTGGTTTTT GTCCTGATAA AGGCAAACCC CCCTTGCCTG 
CTGTCCACTG TGCAGTACAT CAGTAGTTTC TATGCCAACT GCTTGTCTGG AGAGGAGTCG 
TACTGGTGGA TGCAGTTCAC GGCAGCCGTG GAGTTCATCA AAACCATCGA TGATCGCAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPVD1 ( XM_017810052.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017665541.1 Lepidothrix coronata GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X7, mRNA.

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CloneID	OLe54935
Clone ID Related Accession (Same CDS sequence)	XM_017810052.1
Accession Version	XM_017810052.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690208.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGC TGGACATCCA CACGCTGGCC CACCACCTGA AGCAGGAGCG GCTCTACGTG 
ACGGCGGAGA AGCAGCTGAT CCAGCGGCTC AACGCCGACG TGCTGAAGAC GGCGGAGAAG 
CTCTACCGGA CAGCCTGGAT CTCCAAACAG CAGCGCATCA ACCTGGACAG GCTCATCATC 
ACCAGTGCTG AAGCTTCTCC TGCTGAATGT TGCCAACATG CAAAAATCTT GGAGGACACA 
CAGTTTGTGG ATGGATATAA GCAGCTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCCTG 
AACAGACTGA GAGAGAACCC GAGGCTTATT GCATCCTGCC TGGTTGCTGG AGAGAAGCTC 
AACCAGGACA ACACTCAGAG TGTCATTCAC ACAGTCTTTA CCTCCATTTA TGGCAACTGC 
ATCATGCAGG AGGATGAGAG TTACCTGCTC CAGGTCCTCC GTTACCTGAT TGAGTTTGAA 
CTCAAGGAGA GCGACAACCC CCGGCGGCTG CTCCGACGAG GCACCTGTGC CTTCAGCATC 
TTGTTCAAAC TCTTCTCTGA AGGACTCTTC TCTGCAAAAC TTTTTCTTAC TGCAACCTTA 
CATGAGCCAA TCATGCAGCT CCTGGTTGAA GATGAAGACC ACCTGGAAAC CGATGCAAAC 
AAGTTAATTG AGAGATTCTC CCCAGTACAG CAGGAAAAGT TATTTGGAGA GAAAGGCACA 
GAAAAGTTCA AGCAAAGAGT CCAAGAGATG GTTGACTCCA ACGAGGCCAA GCTGGTGACT 
TTGGTCAACA AATTCATTGG CTATCTCAAA CAAAACACTT ACTGTTTTCC TCACAGCTTG 
AGGTGGATTG TGTCGCAGAT GTACAAAACG CTGTCGTGTG TGGACAGGCT GGAGGTTGGG 
GAGGTCAGAG CCATGTGCAC AGATCTTCTT CTGGCCTGTT TCATCTGCCC TGCAATCGTT 
AACCCAGAGC AATATGGGAT CATTTCTGAT GCTCCTATAA ATGAGGTGGC AAGATTTAAT 
CTGATGCAGG TTGGGAGGCT CCTGCAGCAG CTGGCAATGA CAGGCTCTGA GGAGGGAGAT 
CCACGTACCA AGAGCAGCCT TGCTAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTGGAT 
GTGGTCATCG ACGGGCGCGC GGTGGAGACG CCTCCGATGT CTGCTGTCAA CCTGCTGGAG 
GGGCTGAGCA GGACCGTGGT GTACATGACA TACAGCCAGC TGACCACTCT GGTTGGCTTT 
ATGCGCAACG TGATGTCAAG CGATCAGCTT AAGGAAGATC GGATGGCTTT GGAAAACTTG 
CTGGCAAACT TACCCCAGAA CAAGCCAGGG AAAAGCAGCA GCCTTGAAAT GACTCCATAT 
AACACCCCCC AGCTTTCTCC TGCGACCACT CCAGCCAACA AAAAAAACCG GTTACCGATA 
GGACAGCAAT TGGCAGCCAT TACTGCCTGG GACACCTCTG CCACCAATCT GTCAGCTCAC 
ATAACTCTAG TAACCCCTTT TGCAACTCGT AGCAGAAGTA GATCAAATAT TCTGATGGAT 
CAGCACGGAG ATCACGAAGG ATCCTCCCAG GAAACTATCC CAGAAGTTCA GCCAGAAGAA 
GTGCTGGTGA TTTCTCTGGG GACAGGTCCA CAGATTACTC CAGGAATGAT GTCAGAAAAT 
GAGGTCTTAA ACATGCAGCT TGCAGATGGC GGGCAAGATG TCCCCATTGA CGAAAACAAA 
CTCCATGGTA AACCTGATAA AACCTTGCGC TTTTCCCTCT GCAGTGATAA TCTGGAAGGA 
ATATCTGAAG GTCCTTCCAA TCGCTCCAAC TCCGTGTCCT CGCTGGATTT GGAAGGGGAG 
TCGGTGTCGG AGCTCGGCGC GGGCCCCTCC GGGAGCAACG GTGTGGAGGC TCTGCAGCTC 
CTGGAGCATG AACAAGCCAC AACTCAGGAT AATCTGGATG ACAAGCTCCG TAAGTTTGAA 
ATCCGTGATA TGATGGGTTT GACTGATGAC AGAGATATAT CAGAAACTGT GAGTGAAACC 
TGGAGCACAG ATGTCTTGGG AAGTGACTTC GACCCCAACA TGGACGAGGA CCGGCTGCAG 
GAGATCGCAG AGGCTCCAGA CTTAAAACAG GAGGAAAGGC TCCAAGAACT GGAGAGCTGT 
TCTGGGCTGG GCAGCACGTC TGATGACACA GATGTGAGGG AGGTCAGCTC TCGACCCAGC 
ACTCCAGGAC TCAGCGTTGT GTCAGGTATT AGTGCAACAT CTGAAGATAT TCCTAACAAG 
ATTGAGGATC TCAGATCTGA ATGTAGCTCT GACTTTGGGG GAAAAGATTC TGTGACAAGT 
CCTGATATGG ATGAAACAGC CCATGGAGCC AGTCAATTGA CATCTCCACC TTCTCAGACA 
GATTCTTTGC TTGCACTGTT TGACCCTCTG TCCTCAAATG AGGGTGTGTC AGCTGTAGTA 
AGGCCTAAAG TGCACTATGC AAGACCCTCT CACCCACCTC CGGATCCACC CATCCTGGAA 
GGAGCTGTGG GAGGTAACGA GGCCCGACTG CCAAACTTTG GGTCTCACGC TCTGATTCCA 
ACCGATCTGG AAGCCTTCAA GCAGAGACAT TCCTACCCGG AGAGGCTGGT CCGCAGCCGG 
AGCTCAGACA TAGTGTCGTC GGTTCGGAGG CCCATGAGTG ATCCCGGGTG GAACAGGCGT 
CCTGGGAACG AGGACAGGGA GCTGCCCATG GCCACCAGCA GCGCTGGAGC AGCTGTGCTG 
GCAGCCACGT CTCAGTCATC GTCTTCATCT CCCAGTAAGG ACTCCTCCAG GGGAGAGATT 
GAGGAACGAA AAGACAGCGA TGATGAGAAG TCTGACAGGA ACAAGCCCTG GTGGAGGAAA 
CGTTTTGCGT CTGCCATGCC CAAAGCTCCT ATTCCATTTA GAAAGAAAGA AAAACAAGAG 
AAAGACAAAG ATGACATGGT GCCTGACAGA TACGCAACAC TTCAAGATGA TCCCAGCCCA 
AGGCTCAGTG CACAGGCACA AGCTGCTGAG GACATTCTGG ACAAATATAG GAATGCCATC 
AAGAGAACCA GTCCCAGTGA GGGAGCCATA GTGAACTATG ACAGTGCAGA GGCTCTTGGG 
GATGGTGAGA GCATGCACGA CTCCCCGCGG GACGAGGCCT TGCAGAACAT GTCTGCAGAC 
GATCTCCCAG ACTCTGCAAG TCAAGTAGCA CAGCCACAAA GTTCTGCTTT CTCCTATAGG 
GATGCAAAGA AGAAATTGAG ATTGGCTCTT TGTTCAGCAG ATTCTGTTGC CTTCCCGATG 
TTGACCCACT CAACACGGAA TGGTCTCCCA GACCACACAG ACCCCGAAGA TAATGAAATT 
GTGTGCTTCT TGAAAGTTCA GCTGGCTGAA GCCATCAACC TCCAGGACAA GAACCTGATG 
GCACAGCTGC AGGAGACGAT GCGCTGCGTG AGCCGCTTCG ACAACAGGAC CTGTCGGAAG 
CTGCTGGCCT CCATTGCAGA GGACTATAGG AAAAGAGCTC CATATATTGC TTATTTGACT 
CGCTGTCGCC AAGGCCTGCA GACGACACAG GCGCACCTGG AAAGGCTCTT GCAGAGAGTT 
CTACGAGATA AAGAAGTGGC CAACAGATAC TTCACTACAG TATGTGTGAG GTTACTGCTG 
GAGAGCAAAG AAAAGAAAAT AAGGGAATTT ATTCAAGATT TCCAGAAGCT CACAGCAGCA 
GACGATAAAA CAGCCCAAGT TGAGGATTTC CTTCAGTTCC TGTACGGGGC TATGGCTCAG 
GATGCCATAT GGCAGAATGC CAGTGAGGAA CAGCTTCAGG ATGCACAATT AGCCATCGAG 
CGCAGTGTGA TGAATCGCAT TTTCAAACTT GCCTTCTACC CTAATCAGGA TGGAGATATT 
CTGCGTGACC AGGTCCTTCA CGAGCACATA CAGAGGTTAT CCAAAGTAGT GACTGCAAAC 
CACAAGGCCC TTCAGATACC TGAGGTGTAT CTCCGGGAGG CACCGTGGCC GTCGGCACAG 
TCCGAGATCC GCACGATAAG TGCTTACAAA ACCCCCCGGG ACAAGGTGCA GTGTATCCTG 
AGGATGTGCT CCACCATCAT GAACCTGCTC AGCCTGGCCA ACGAGGATTC AGTACCTGGG 
GCAGATGATT TTGTTCCTGT TTTGGTTTTT GTCCTGATAA AGGCAAACCC CCCTTGCCTG 
CTGTCCACTG TGCAGTACAT CAGTAGTTTC TATGCCAACT GCTTGTCTGG AGAGGAGTCG 
TACTGGTGGA TGCAGTTCAC GGCAGCCGTG GAGTTCATCA AAACCATCGA TGATCGCAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017665541.1
CDS	162..4484
Translation	MVKLDIHTLAHHLKQERLYVTAEKQLIQRLNADVLKTAEKLYRTAWISKQQRINLDRLIITSAEASPAECCQHAKILEDTQFVDGYKQLGFQETAYGEFLNRLRENPRLIASCLVAGEKLNQDNTQSVIHTVFTSIYGNCIMQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDANKLIERFSPVQQEKLFGEKGTEKFKQRVQEMVDSNEAKLVTLVNKFIGYLKQNTYCFPHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAIVNPEQYGIISDAPINEVARFNLMQVGRLLQQLAMTGSEEGDPRTKSSLAKFDKSCVAAFLDVVIDGRAVETPPMSAVNLLEGLSRTVVYMTYSQLTTLVGFMRNVMSSDQLKEDRMALENLLANLPQNKPGKSSSLEMTPYNTPQLSPATTPANKKNRLPIGQQLAAITAWDTSATNLSAHITLVTPFATRSRSRSNILMDQHGDHEGSSQETIPEVQPEEVLVISLGTGPQITPGMMSENEVLNMQLADGGQDVPIDENKLHGKPDKTLRFSLCSDNLEGISEGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNMDEDRLQEIAEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDETAHGASQLTSPPSQTDSLLALFDPLSSNEGVSAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHALIPTDLEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPGWNRRPGNEDRELPMATSSAGAAVLAATSQSSSSSPSKDSSRGEIEERKDSDDEKSDRNKPWWRKRFASAMPKAPIPFRKKEKQEKDKDDMVPDRYATLQDDPSPRLSAQAQAAEDILDKYRNAIKRTSPSEGAIVNYDSAEALGDGESMHDSPRDEALQNMSADDLPDSASQVAQPQSSAFSYRDAKKKLRLALCSADSVAFPMLTHSTRNGLPDHTDPEDNEIVCFLKVQLAEAINLQDKNLMAQLQETMRCVSRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGAMAQDAIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHKALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYANCLSGEESYWWMQFTAAVEFIKTIDDRK

Target ORF information:

RefSeq Version	XM_017810052.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017810052.1

ORF Insert Sequence:

ATGGTGAAGC TGGACATCCA CACGCTGGCC CACCACCTGA AGCAGGAGCG GCTCTACGTG 
ACGGCGGAGA AGCAGCTGAT CCAGCGGCTC AACGCCGACG TGCTGAAGAC GGCGGAGAAG 
CTCTACCGGA CAGCCTGGAT CTCCAAACAG CAGCGCATCA ACCTGGACAG GCTCATCATC 
ACCAGTGCTG AAGCTTCTCC TGCTGAATGT TGCCAACATG CAAAAATCTT GGAGGACACA 
CAGTTTGTGG ATGGATATAA GCAGCTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCCTG 
AACAGACTGA GAGAGAACCC GAGGCTTATT GCATCCTGCC TGGTTGCTGG AGAGAAGCTC 
AACCAGGACA ACACTCAGAG TGTCATTCAC ACAGTCTTTA CCTCCATTTA TGGCAACTGC 
ATCATGCAGG AGGATGAGAG TTACCTGCTC CAGGTCCTCC GTTACCTGAT TGAGTTTGAA 
CTCAAGGAGA GCGACAACCC CCGGCGGCTG CTCCGACGAG GCACCTGTGC CTTCAGCATC 
TTGTTCAAAC TCTTCTCTGA AGGACTCTTC TCTGCAAAAC TTTTTCTTAC TGCAACCTTA 
CATGAGCCAA TCATGCAGCT CCTGGTTGAA GATGAAGACC ACCTGGAAAC CGATGCAAAC 
AAGTTAATTG AGAGATTCTC CCCAGTACAG CAGGAAAAGT TATTTGGAGA GAAAGGCACA 
GAAAAGTTCA AGCAAAGAGT CCAAGAGATG GTTGACTCCA ACGAGGCCAA GCTGGTGACT 
TTGGTCAACA AATTCATTGG CTATCTCAAA CAAAACACTT ACTGTTTTCC TCACAGCTTG 
AGGTGGATTG TGTCGCAGAT GTACAAAACG CTGTCGTGTG TGGACAGGCT GGAGGTTGGG 
GAGGTCAGAG CCATGTGCAC AGATCTTCTT CTGGCCTGTT TCATCTGCCC TGCAATCGTT 
AACCCAGAGC AATATGGGAT CATTTCTGAT GCTCCTATAA ATGAGGTGGC AAGATTTAAT 
CTGATGCAGG TTGGGAGGCT CCTGCAGCAG CTGGCAATGA CAGGCTCTGA GGAGGGAGAT 
CCACGTACCA AGAGCAGCCT TGCTAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTGGAT 
GTGGTCATCG ACGGGCGCGC GGTGGAGACG CCTCCGATGT CTGCTGTCAA CCTGCTGGAG 
GGGCTGAGCA GGACCGTGGT GTACATGACA TACAGCCAGC TGACCACTCT GGTTGGCTTT 
ATGCGCAACG TGATGTCAAG CGATCAGCTT AAGGAAGATC GGATGGCTTT GGAAAACTTG 
CTGGCAAACT TACCCCAGAA CAAGCCAGGG AAAAGCAGCA GCCTTGAAAT GACTCCATAT 
AACACCCCCC AGCTTTCTCC TGCGACCACT CCAGCCAACA AAAAAAACCG GTTACCGATA 
GGACAGCAAT TGGCAGCCAT TACTGCCTGG GACACCTCTG CCACCAATCT GTCAGCTCAC 
ATAACTCTAG TAACCCCTTT TGCAACTCGT AGCAGAAGTA GATCAAATAT TCTGATGGAT 
CAGCACGGAG ATCACGAAGG ATCCTCCCAG GAAACTATCC CAGAAGTTCA GCCAGAAGAA 
GTGCTGGTGA TTTCTCTGGG GACAGGTCCA CAGATTACTC CAGGAATGAT GTCAGAAAAT 
GAGGTCTTAA ACATGCAGCT TGCAGATGGC GGGCAAGATG TCCCCATTGA CGAAAACAAA 
CTCCATGGTA AACCTGATAA AACCTTGCGC TTTTCCCTCT GCAGTGATAA TCTGGAAGGA 
ATATCTGAAG GTCCTTCCAA TCGCTCCAAC TCCGTGTCCT CGCTGGATTT GGAAGGGGAG 
TCGGTGTCGG AGCTCGGCGC GGGCCCCTCC GGGAGCAACG GTGTGGAGGC TCTGCAGCTC 
CTGGAGCATG AACAAGCCAC AACTCAGGAT AATCTGGATG ACAAGCTCCG TAAGTTTGAA 
ATCCGTGATA TGATGGGTTT GACTGATGAC AGAGATATAT CAGAAACTGT GAGTGAAACC 
TGGAGCACAG ATGTCTTGGG AAGTGACTTC GACCCCAACA TGGACGAGGA CCGGCTGCAG 
GAGATCGCAG AGGCTCCAGA CTTAAAACAG GAGGAAAGGC TCCAAGAACT GGAGAGCTGT 
TCTGGGCTGG GCAGCACGTC TGATGACACA GATGTGAGGG AGGTCAGCTC TCGACCCAGC 
ACTCCAGGAC TCAGCGTTGT GTCAGGTATT AGTGCAACAT CTGAAGATAT TCCTAACAAG 
ATTGAGGATC TCAGATCTGA ATGTAGCTCT GACTTTGGGG GAAAAGATTC TGTGACAAGT 
CCTGATATGG ATGAAACAGC CCATGGAGCC AGTCAATTGA CATCTCCACC TTCTCAGACA 
GATTCTTTGC TTGCACTGTT TGACCCTCTG TCCTCAAATG AGGGTGTGTC AGCTGTAGTA 
AGGCCTAAAG TGCACTATGC AAGACCCTCT CACCCACCTC CGGATCCACC CATCCTGGAA 
GGAGCTGTGG GAGGTAACGA GGCCCGACTG CCAAACTTTG GGTCTCACGC TCTGATTCCA 
ACCGATCTGG AAGCCTTCAA GCAGAGACAT TCCTACCCGG AGAGGCTGGT CCGCAGCCGG 
AGCTCAGACA TAGTGTCGTC GGTTCGGAGG CCCATGAGTG ATCCCGGGTG GAACAGGCGT 
CCTGGGAACG AGGACAGGGA GCTGCCCATG GCCACCAGCA GCGCTGGAGC AGCTGTGCTG 
GCAGCCACGT CTCAGTCATC GTCTTCATCT CCCAGTAAGG ACTCCTCCAG GGGAGAGATT 
GAGGAACGAA AAGACAGCGA TGATGAGAAG TCTGACAGGA ACAAGCCCTG GTGGAGGAAA 
CGTTTTGCGT CTGCCATGCC CAAAGCTCCT ATTCCATTTA GAAAGAAAGA AAAACAAGAG 
AAAGACAAAG ATGACATGGT GCCTGACAGA TACGCAACAC TTCAAGATGA TCCCAGCCCA 
AGGCTCAGTG CACAGGCACA AGCTGCTGAG GACATTCTGG ACAAATATAG GAATGCCATC 
AAGAGAACCA GTCCCAGTGA GGGAGCCATA GTGAACTATG ACAGTGCAGA GGCTCTTGGG 
GATGGTGAGA GCATGCACGA CTCCCCGCGG GACGAGGCCT TGCAGAACAT GTCTGCAGAC 
GATCTCCCAG ACTCTGCAAG TCAAGTAGCA CAGCCACAAA GTTCTGCTTT CTCCTATAGG 
GATGCAAAGA AGAAATTGAG ATTGGCTCTT TGTTCAGCAG ATTCTGTTGC CTTCCCGATG 
TTGACCCACT CAACACGGAA TGGTCTCCCA GACCACACAG ACCCCGAAGA TAATGAAATT 
GTGTGCTTCT TGAAAGTTCA GCTGGCTGAA GCCATCAACC TCCAGGACAA GAACCTGATG 
GCACAGCTGC AGGAGACGAT GCGCTGCGTG AGCCGCTTCG ACAACAGGAC CTGTCGGAAG 
CTGCTGGCCT CCATTGCAGA GGACTATAGG AAAAGAGCTC CATATATTGC TTATTTGACT 
CGCTGTCGCC AAGGCCTGCA GACGACACAG GCGCACCTGG AAAGGCTCTT GCAGAGAGTT 
CTACGAGATA AAGAAGTGGC CAACAGATAC TTCACTACAG TATGTGTGAG GTTACTGCTG 
GAGAGCAAAG AAAAGAAAAT AAGGGAATTT ATTCAAGATT TCCAGAAGCT CACAGCAGCA 
GACGATAAAA CAGCCCAAGT TGAGGATTTC CTTCAGTTCC TGTACGGGGC TATGGCTCAG 
GATGCCATAT GGCAGAATGC CAGTGAGGAA CAGCTTCAGG ATGCACAATT AGCCATCGAG 
CGCAGTGTGA TGAATCGCAT TTTCAAACTT GCCTTCTACC CTAATCAGGA TGGAGATATT 
CTGCGTGACC AGGTCCTTCA CGAGCACATA CAGAGGTTAT CCAAAGTAGT GACTGCAAAC 
CACAAGGCCC TTCAGATACC TGAGGTGTAT CTCCGGGAGG CACCGTGGCC GTCGGCACAG 
TCCGAGATCC GCACGATAAG TGCTTACAAA ACCCCCCGGG ACAAGGTGCA GTGTATCCTG 
AGGATGTGCT CCACCATCAT GAACCTGCTC AGCCTGGCCA ACGAGGATTC AGTACCTGGG 
GCAGATGATT TTGTTCCTGT TTTGGTTTTT GTCCTGATAA AGGCAAACCC CCCTTGCCTG 
CTGTCCACTG TGCAGTACAT CAGTAGTTTC TATGCCAACT GCTTGTCTGG AGAGGAGTCG 
TACTGGTGGA TGCAGTTCAC GGCAGCCGTG GAGTTCATCA AAACCATCGA TGATCGCAAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GAPVD1 ( XM_017810053.1 ) cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin) -> XP_017665542.1 Lepidothrix coronata GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X8, mRNA.

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CloneID	OLe54936
Clone ID Related Accession (Same CDS sequence)	XM_017810053.1
Accession Version	XM_017810053.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4320bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-10
Organism	Lepidothrix coronata(blue-crowned manakin)
Product	GTPase-activating protein and VPS9 domain-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_016690208.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Lepidothrix coronata Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGC TGGACATCCA CACGCTGGCC CACCACCTGA AGCAGGAGCG GCTCTACGTG 
ACGGCGGAGA AGCAGCTGAT CCAGCGGCTC AACGCCGACG TGCTGAAGAC GGCGGAGAAG 
CTCTACCGGA CAGCCTGGAT CTCCAAACAG CAGCGCATCA ACCTGGACAG GCTCATCATC 
ACCAGTGCTG AAGCTTCTCC TGCTGAATGT TGCCAACATG CAAAAATCTT GGAGGACACA 
CAGTTTGTGG ATGGATATAA GCAGCTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCCTG 
AACAGACTGA GAGAGAACCC GAGGCTTATT GCATCCTGCC TGGTTGCTGG AGAGAAGCTC 
AACCAGGACA ACACTCAGAG TGTCATTCAC ACAGTCTTTA CCTCCATTTA TGGCAACTGC 
ATCATGCAGG AGGATGAGAG TTACCTGCTC CAGGTCCTCC GTTACCTGAT TGAGTTTGAA 
CTCAAGGAGA GCGACAACCC CCGGCGGCTG CTCCGACGAG GCACCTGTGC CTTCAGCATC 
TTGTTCAAAC TCTTCTCTGA AGGACTCTTC TCTGCAAAAC TTTTTCTTAC TGCAACCTTA 
CATGAGCCAA TCATGCAGCT CCTGGTTGAA GATGAAGACC ACCTGGAAAC CGATGCAAAC 
AAGTTAATTG AGAGATTCTC CCCAGTACAG CAGGAAAAGT TATTTGGAGA GAAAGGCACA 
GAAAAGTTCA AGCAAAGAGT CCAAGAGATG GTTGACTCCA ACGAGGCCAA GCTGGTGACT 
TTGGTCAACA AATTCATTGG CTATCTCAAA CAAAACACTT ACTGTTTTCC TCACAGCTTG 
AGGTGGATTG TGTCGCAGAT GTACAAAACG CTGTCGTGTG TGGACAGGCT GGAGGTTGGG 
GAGGTCAGAG CCATGTGCAC AGATCTTCTT CTGGCCTGTT TCATCTGCCC TGCAATCGTT 
AACCCAGAGC AATATGGGAT CATTTCTGAT GCTCCTATAA ATGAGGTGGC AAGATTTAAT 
CTGATGCAGG TTGGGAGGCT CCTGCAGCAG CTGGCAATGA CAGGCTCTGA GGAGGGAGAT 
CCACGTACCA AGAGCAGCCT TGCTAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTGGAT 
GTGGTCATCG ACGGGCGCGC GGTGGAGACG CCTCCGATGT CTGCTGTCAA CCTGCTGGAG 
GGGCTGAGCA GGACCGTGGT GTACATGACA TACAGCCAGC TGACCACTCT GGTTGGCTTT 
ATGCGCAACG TGATGTCAAG CGATCAGCTT AAGGAAGATC GGATGGCTTT GGAAAACTTG 
CTGGCAAACT TACCCCAGAA CAAGCCAGGG AAAAGCAGCA GCCTTGAAAT GACTCCATAT 
AACACCCCCC AGCTTTCTCC TGCGACCACT CCAGCCAACA AAAAAAACCG GTTACCGATA 
GCAACTCGTA GCAGAAGTAG ATCAAATATT CTGATGGATC AGCACGGAGA TCACGAAGGA 
TCCTCCCAGG AAACTATCCC AGAAGTTCAG CCAGAAGAAG TGCTGGTGAT TTCTCTGGGG 
ACAGGTCCAC AGATTACTCC AGGAATGATG TCAGAAAATG AGGTCTTAAA CATGCAGCTT 
GCAGATGGCG GGCAAGATGT CCCCATTGAC GAAAACAAAC TCCATGGTCC TTCCAATCGC 
TCCAACTCCG TGTCCTCGCT GGATTTGGAA GGGGAGTCGG TGTCGGAGCT CGGCGCGGGC 
CCCTCCGGGA GCAACGGTGT GGAGGCTCTG CAGCTCCTGG AGCATGAACA AGCCACAACT 
CAGGATAATC TGGATGACAA GCTCCGTAAG TTTGAAATCC GTGATATGAT GGGTTTGACT 
GATGACAGAG ATATATCAGA AACTGTGAGT GAAACCTGGA GCACAGATGT CTTGGGAAGT 
GACTTCGACC CCAACATGGA CGAGGACCGG CTGCAGGAGA TCGCAGGTGC AGCTGCAGAG 
AACATGCTAG GCAGCTTGCT GTGTTTGCCA GGTTCAGGAT CCGTGTTGCT TGATCCCTGC 
ACAGGTTCAA CCATATCAGA AACCACAAGT GAGGCGTGGA GTGTGGAAGT ATTACCGAGT 
GATTCAGAGG CTCCAGACTT AAAACAGGAG GAAAGGCTCC AAGAACTGGA GAGCTGTTCT 
GGGCTGGGCA GCACGTCTGA TGACACAGAT GTGAGGGAGG TCAGCTCTCG ACCCAGCACT 
CCAGGACTCA GCGTTGTGTC AGGTATTAGT GCAACATCTG AAGATATTCC TAACAAGATT 
GAGGATCTCA GATCTGAATG TAGCTCTGAC TTTGGGGGAA AAGATTCTGT GACAAGTCCT 
GATATGGATG AAACAGCCCA TGGAGCCAGT CAATTGACAT CTCCACCTTC TCAGACAGAT 
TCTTTGCTTG CACTGTTTGA CCCTCTGTCC TCAAATGAGG GTGTGTCAGC TGTAGTAAGG 
CCTAAAGTGC ACTATGCAAG ACCCTCTCAC CCACCTCCGG ATCCACCCAT CCTGGAAGGA 
GCTGTGGGAG GTAACGAGGC CCGACTGCCA AACTTTGGGT CTCACGCTCT GATTCCAACC 
GATCTGGAAG CCTTCAAGCA GAGACATTCC TACCCGGAGA GGCTGGTCCG CAGCCGGAGC 
TCAGACATAG TGTCGTCGGT TCGGAGGCCC ATGAGTGATC CCGGGTGGAA CAGGCGTCCT 
GGGAACGAGG ACAGGGAGCT GCCCATGGCC ACCAGCAGCG CTGGAGCAGC TGTGCTGGCA 
GCCACGTCTC AGTCATCGTC TTCATCTCCC AGTAAGGACT CCTCCAGGGG AGAGATTGAG 
GAACGAAAAG ACAGCGATGA TGAGAAGTCT GACAGGAACA AGCCCTGGTG GAGGAAACGT 
TTTGCGTCTG CCATGCCCAA AGCTCCTATT CCATTTAGAA AGAAAGAAAA ACAAGAGAAA 
GACAAAGATG ACATGGTGCC TGACAGATAC GCAACACTTC AAGATGATCC CAGCCCAAGG 
CTCAGTGCAC AGGCACAAGC TGCTGAGGAC ATTCTGGACA AATATAGGAA TGCCATCAAG 
AGAACCAGTC CCAGTGAGGG AGCCATAGTG AACTATGACA GTGCAGAGGC TCTTGGGGAT 
GGTGAGAGCA TGCACGACTC CCCGCGGGAC GAGGCCTTGC AGAACATGTC TGCAGACGAT 
CTCCCAGACT CTGCAAGTCA AGTAGCACAG CCACAAAGTT CTGCTTTCTC CTATAGGGAT 
GCAAAGAAGA AATTGAGATT GGCTCTTTGT TCAGCAGATT CTGTTGCCTT CCCGATGTTG 
ACCCACTCAA CACGGAATGG TCTCCCAGAC CACACAGACC CCGAAGATAA TGAAATTGTG 
TGCTTCTTGA AAGTTCAGCT GGCTGAAGCC ATCAACCTCC AGGACAAGAA CCTGATGGCA 
CAGCTGCAGG AGACGATGCG CTGCGTGAGC CGCTTCGACA ACAGGACCTG TCGGAAGCTG 
CTGGCCTCCA TTGCAGAGGA CTATAGGAAA AGAGCTCCAT ATATTGCTTA TTTGACTCGC 
TGTCGCCAAG GCCTGCAGAC GACACAGGCG CACCTGGAAA GGCTCTTGCA GAGAGTTCTA 
CGAGATAAAG AAGTGGCCAA CAGATACTTC ACTACAGTAT GTGTGAGGTT ACTGCTGGAG 
AGCAAAGAAA AGAAAATAAG GGAATTTATT CAAGATTTCC AGAAGCTCAC AGCAGCAGAC 
GATAAAACAG CCCAAGTTGA GGATTTCCTT CAGTTCCTGT ACGGGGCTAT GGCTCAGGAT 
GCCATATGGC AGAATGCCAG TGAGGAACAG CTTCAGGATG CACAATTAGC CATCGAGCGC 
AGTGTGATGA ATCGCATTTT CAAACTTGCC TTCTACCCTA ATCAGGATGG AGATATTCTG 
CGTGACCAGG TCCTTCACGA GCACATACAG AGGTTATCCA AAGTAGTGAC TGCAAACCAC 
AAGGCCCTTC AGATACCTGA GGTGTATCTC CGGGAGGCAC CGTGGCCGTC GGCACAGTCC 
GAGATCCGCA CGATAAGTGC TTACAAAACC CCCCGGGACA AGGTGCAGTG TATCCTGAGG 
ATGTGCTCCA CCATCATGAA CCTGCTCAGC CTGGCCAACG AGGATTCAGT ACCTGGGGCA 
GATGATTTTG TTCCTGTTTT GGTTTTTGTC CTGATAAAGG CAAACCCCCC TTGCCTGCTG 
TCCACTGTGC AGTACATCAG TAGTTTCTAT GCCAACTGCT TGTCTGGAGA GGAGTCGTAC 
TGGTGGATGC AGTTCACGGC AGCCGTGGAG TTCATCAAAA CCATCGATGA TCGCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017665542.1
CDS	162..4481
Translation	MVKLDIHTLAHHLKQERLYVTAEKQLIQRLNADVLKTAEKLYRTAWISKQQRINLDRLIITSAEASPAECCQHAKILEDTQFVDGYKQLGFQETAYGEFLNRLRENPRLIASCLVAGEKLNQDNTQSVIHTVFTSIYGNCIMQEDESYLLQVLRYLIEFELKESDNPRRLLRRGTCAFSILFKLFSEGLFSAKLFLTATLHEPIMQLLVEDEDHLETDANKLIERFSPVQQEKLFGEKGTEKFKQRVQEMVDSNEAKLVTLVNKFIGYLKQNTYCFPHSLRWIVSQMYKTLSCVDRLEVGEVRAMCTDLLLACFICPAIVNPEQYGIISDAPINEVARFNLMQVGRLLQQLAMTGSEEGDPRTKSSLAKFDKSCVAAFLDVVIDGRAVETPPMSAVNLLEGLSRTVVYMTYSQLTTLVGFMRNVMSSDQLKEDRMALENLLANLPQNKPGKSSSLEMTPYNTPQLSPATTPANKKNRLPIATRSRSRSNILMDQHGDHEGSSQETIPEVQPEEVLVISLGTGPQITPGMMSENEVLNMQLADGGQDVPIDENKLHGPSNRSNSVSSLDLEGESVSELGAGPSGSNGVEALQLLEHEQATTQDNLDDKLRKFEIRDMMGLTDDRDISETVSETWSTDVLGSDFDPNMDEDRLQEIAGAAAENMLGSLLCLPGSGSVLLDPCTGSTISETTSEAWSVEVLPSDSEAPDLKQEERLQELESCSGLGSTSDDTDVREVSSRPSTPGLSVVSGISATSEDIPNKIEDLRSECSSDFGGKDSVTSPDMDETAHGASQLTSPPSQTDSLLALFDPLSSNEGVSAVVRPKVHYARPSHPPPDPPILEGAVGGNEARLPNFGSHALIPTDLEAFKQRHSYPERLVRSRSSDIVSSVRRPMSDPGWNRRPGNEDRELPMATSSAGAAVLAATSQSSSSSPSKDSSRGEIEERKDSDDEKSDRNKPWWRKRFASAMPKAPIPFRKKEKQEKDKDDMVPDRYATLQDDPSPRLSAQAQAAEDILDKYRNAIKRTSPSEGAIVNYDSAEALGDGESMHDSPRDEALQNMSADDLPDSASQVAQPQSSAFSYRDAKKKLRLALCSADSVAFPMLTHSTRNGLPDHTDPEDNEIVCFLKVQLAEAINLQDKNLMAQLQETMRCVSRFDNRTCRKLLASIAEDYRKRAPYIAYLTRCRQGLQTTQAHLERLLQRVLRDKEVANRYFTTVCVRLLLESKEKKIREFIQDFQKLTAADDKTAQVEDFLQFLYGAMAQDAIWQNASEEQLQDAQLAIERSVMNRIFKLAFYPNQDGDILRDQVLHEHIQRLSKVVTANHKALQIPEVYLREAPWPSAQSEIRTISAYKTPRDKVQCILRMCSTIMNLLSLANEDSVPGADDFVPVLVFVLIKANPPCLLSTVQYISSFYANCLSGEESYWWMQFTAAVEFIKTIDDRK

Target ORF information:

RefSeq Version	XM_017810053.1
Organism	Lepidothrix coronata(blue-crowned manakin)
Definition	Lepidothrix coronata GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017810053.1

ORF Insert Sequence:

ATGGTGAAGC TGGACATCCA CACGCTGGCC CACCACCTGA AGCAGGAGCG GCTCTACGTG 
ACGGCGGAGA AGCAGCTGAT CCAGCGGCTC AACGCCGACG TGCTGAAGAC GGCGGAGAAG 
CTCTACCGGA CAGCCTGGAT CTCCAAACAG CAGCGCATCA ACCTGGACAG GCTCATCATC 
ACCAGTGCTG AAGCTTCTCC TGCTGAATGT TGCCAACATG CAAAAATCTT GGAGGACACA 
CAGTTTGTGG ATGGATATAA GCAGCTGGGA TTTCAGGAGA CTGCTTATGG AGAATTCCTG 
AACAGACTGA GAGAGAACCC GAGGCTTATT GCATCCTGCC TGGTTGCTGG AGAGAAGCTC 
AACCAGGACA ACACTCAGAG TGTCATTCAC ACAGTCTTTA CCTCCATTTA TGGCAACTGC 
ATCATGCAGG AGGATGAGAG TTACCTGCTC CAGGTCCTCC GTTACCTGAT TGAGTTTGAA 
CTCAAGGAGA GCGACAACCC CCGGCGGCTG CTCCGACGAG GCACCTGTGC CTTCAGCATC 
TTGTTCAAAC TCTTCTCTGA AGGACTCTTC TCTGCAAAAC TTTTTCTTAC TGCAACCTTA 
CATGAGCCAA TCATGCAGCT CCTGGTTGAA GATGAAGACC ACCTGGAAAC CGATGCAAAC 
AAGTTAATTG AGAGATTCTC CCCAGTACAG CAGGAAAAGT TATTTGGAGA GAAAGGCACA 
GAAAAGTTCA AGCAAAGAGT CCAAGAGATG GTTGACTCCA ACGAGGCCAA GCTGGTGACT 
TTGGTCAACA AATTCATTGG CTATCTCAAA CAAAACACTT ACTGTTTTCC TCACAGCTTG 
AGGTGGATTG TGTCGCAGAT GTACAAAACG CTGTCGTGTG TGGACAGGCT GGAGGTTGGG 
GAGGTCAGAG CCATGTGCAC AGATCTTCTT CTGGCCTGTT TCATCTGCCC TGCAATCGTT 
AACCCAGAGC AATATGGGAT CATTTCTGAT GCTCCTATAA ATGAGGTGGC AAGATTTAAT 
CTGATGCAGG TTGGGAGGCT CCTGCAGCAG CTGGCAATGA CAGGCTCTGA GGAGGGAGAT 
CCACGTACCA AGAGCAGCCT TGCTAAATTT GACAAAAGCT GTGTTGCTGC TTTCCTGGAT 
GTGGTCATCG ACGGGCGCGC GGTGGAGACG CCTCCGATGT CTGCTGTCAA CCTGCTGGAG 
GGGCTGAGCA GGACCGTGGT GTACATGACA TACAGCCAGC TGACCACTCT GGTTGGCTTT 
ATGCGCAACG TGATGTCAAG CGATCAGCTT AAGGAAGATC GGATGGCTTT GGAAAACTTG 
CTGGCAAACT TACCCCAGAA CAAGCCAGGG AAAAGCAGCA GCCTTGAAAT GACTCCATAT 
AACACCCCCC AGCTTTCTCC TGCGACCACT CCAGCCAACA AAAAAAACCG GTTACCGATA 
GCAACTCGTA GCAGAAGTAG ATCAAATATT CTGATGGATC AGCACGGAGA TCACGAAGGA 
TCCTCCCAGG AAACTATCCC AGAAGTTCAG CCAGAAGAAG TGCTGGTGAT TTCTCTGGGG 
ACAGGTCCAC AGATTACTCC AGGAATGATG TCAGAAAATG AGGTCTTAAA CATGCAGCTT 
GCAGATGGCG GGCAAGATGT CCCCATTGAC GAAAACAAAC TCCATGGTCC TTCCAATCGC 
TCCAACTCCG TGTCCTCGCT GGATTTGGAA GGGGAGTCGG TGTCGGAGCT CGGCGCGGGC 
CCCTCCGGGA GCAACGGTGT GGAGGCTCTG CAGCTCCTGG AGCATGAACA AGCCACAACT 
CAGGATAATC TGGATGACAA GCTCCGTAAG TTTGAAATCC GTGATATGAT GGGTTTGACT 
GATGACAGAG ATATATCAGA AACTGTGAGT GAAACCTGGA GCACAGATGT CTTGGGAAGT 
GACTTCGACC CCAACATGGA CGAGGACCGG CTGCAGGAGA TCGCAGGTGC AGCTGCAGAG 
AACATGCTAG GCAGCTTGCT GTGTTTGCCA GGTTCAGGAT CCGTGTTGCT TGATCCCTGC 
ACAGGTTCAA CCATATCAGA AACCACAAGT GAGGCGTGGA GTGTGGAAGT ATTACCGAGT 
GATTCAGAGG CTCCAGACTT AAAACAGGAG GAAAGGCTCC AAGAACTGGA GAGCTGTTCT 
GGGCTGGGCA GCACGTCTGA TGACACAGAT GTGAGGGAGG TCAGCTCTCG ACCCAGCACT 
CCAGGACTCA GCGTTGTGTC AGGTATTAGT GCAACATCTG AAGATATTCC TAACAAGATT 
GAGGATCTCA GATCTGAATG TAGCTCTGAC TTTGGGGGAA AAGATTCTGT GACAAGTCCT 
GATATGGATG AAACAGCCCA TGGAGCCAGT CAATTGACAT CTCCACCTTC TCAGACAGAT 
TCTTTGCTTG CACTGTTTGA CCCTCTGTCC TCAAATGAGG GTGTGTCAGC TGTAGTAAGG 
CCTAAAGTGC ACTATGCAAG ACCCTCTCAC CCACCTCCGG ATCCACCCAT CCTGGAAGGA 
GCTGTGGGAG GTAACGAGGC CCGACTGCCA AACTTTGGGT CTCACGCTCT GATTCCAACC 
GATCTGGAAG CCTTCAAGCA GAGACATTCC TACCCGGAGA GGCTGGTCCG CAGCCGGAGC 
TCAGACATAG TGTCGTCGGT TCGGAGGCCC ATGAGTGATC CCGGGTGGAA CAGGCGTCCT 
GGGAACGAGG ACAGGGAGCT GCCCATGGCC ACCAGCAGCG CTGGAGCAGC TGTGCTGGCA 
GCCACGTCTC AGTCATCGTC TTCATCTCCC AGTAAGGACT CCTCCAGGGG AGAGATTGAG 
GAACGAAAAG ACAGCGATGA TGAGAAGTCT GACAGGAACA AGCCCTGGTG GAGGAAACGT 
TTTGCGTCTG CCATGCCCAA AGCTCCTATT CCATTTAGAA AGAAAGAAAA ACAAGAGAAA 
GACAAAGATG ACATGGTGCC TGACAGATAC GCAACACTTC AAGATGATCC CAGCCCAAGG 
CTCAGTGCAC AGGCACAAGC TGCTGAGGAC ATTCTGGACA AATATAGGAA TGCCATCAAG 
AGAACCAGTC CCAGTGAGGG AGCCATAGTG AACTATGACA GTGCAGAGGC TCTTGGGGAT 
GGTGAGAGCA TGCACGACTC CCCGCGGGAC GAGGCCTTGC AGAACATGTC TGCAGACGAT 
CTCCCAGACT CTGCAAGTCA AGTAGCACAG CCACAAAGTT CTGCTTTCTC CTATAGGGAT 
GCAAAGAAGA AATTGAGATT GGCTCTTTGT TCAGCAGATT CTGTTGCCTT CCCGATGTTG 
ACCCACTCAA CACGGAATGG TCTCCCAGAC CACACAGACC CCGAAGATAA TGAAATTGTG 
TGCTTCTTGA AAGTTCAGCT GGCTGAAGCC ATCAACCTCC AGGACAAGAA CCTGATGGCA 
CAGCTGCAGG AGACGATGCG CTGCGTGAGC CGCTTCGACA ACAGGACCTG TCGGAAGCTG 
CTGGCCTCCA TTGCAGAGGA CTATAGGAAA AGAGCTCCAT ATATTGCTTA TTTGACTCGC 
TGTCGCCAAG GCCTGCAGAC GACACAGGCG CACCTGGAAA GGCTCTTGCA GAGAGTTCTA 
CGAGATAAAG AAGTGGCCAA CAGATACTTC ACTACAGTAT GTGTGAGGTT ACTGCTGGAG 
AGCAAAGAAA AGAAAATAAG GGAATTTATT CAAGATTTCC AGAAGCTCAC AGCAGCAGAC 
GATAAAACAG CCCAAGTTGA GGATTTCCTT CAGTTCCTGT ACGGGGCTAT GGCTCAGGAT 
GCCATATGGC AGAATGCCAG TGAGGAACAG CTTCAGGATG CACAATTAGC CATCGAGCGC 
AGTGTGATGA ATCGCATTTT CAAACTTGCC TTCTACCCTA ATCAGGATGG AGATATTCTG 
CGTGACCAGG TCCTTCACGA GCACATACAG AGGTTATCCA AAGTAGTGAC TGCAAACCAC 
AAGGCCCTTC AGATACCTGA GGTGTATCTC CGGGAGGCAC CGTGGCCGTC GGCACAGTCC 
GAGATCCGCA CGATAAGTGC TTACAAAACC CCCCGGGACA AGGTGCAGTG TATCCTGAGG 
ATGTGCTCCA CCATCATGAA CCTGCTCAGC CTGGCCAACG AGGATTCAGT ACCTGGGGCA 
GATGATTTTG TTCCTGTTTT GGTTTTTGTC CTGATAAAGG CAAACCCCCC TTGCCTGCTG 
TCCACTGTGC AGTACATCAG TAGTTTCTAT GCCAACTGCT TGTCTGGAGA GGAGTCGTAC 
TGGTGGATGC AGTTCACGGC AGCCGTGGAG TTCATCAAAA CCATCGATGA TCGCAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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見積依頼

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[email protected]

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

GAPVD1 cDNA ORF clone, Lepidothrix coronata(blue-crowned manakin)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム