POLI cDNA ORF clone, Latimeria chalumnae(coelacanth)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLI
Entrez Gene ID	102364192
Full Name	DNA polymerase iota
Gene Type	protein-coding
Organism	Latimeria chalumnae(coelacanth)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005994653.2	XP_005994715.2	LOW QUALITY PROTEIN: DNA polymerase iota

Pathways from BioSystems
Gene Ontology

The following POLI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLa25545	XM_005994653.2 Latest version!	Latimeria chalumnae polymerase (DNA directed) iota (POLI), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLI ( XM_005994653.2 ) cDNA ORF clone, Latimeria chalumnae(coelacanth) -> XP_005994715.2 Latimeria chalumnae polymerase (DNA directed) iota (POLI), mRNA.

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CloneID	OLa25545
Clone ID Related Accession (Same CDS sequence)	XM_005994653.2
Accession Version	XM_005994653.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-10-25
Organism	Latimeria chalumnae(coelacanth)
Product	LOW QUALITY PROTEIN: DNA polymerase iota
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005819226.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Oct 26, 2015 this sequence version replaced XM_005994653.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Latimeria chalumnae Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## internal stop codons :: corrected 1 genomic stop codon ##RefSeq-Attributes-END##

ATGGAATTCC TAGAGCCCGG GAGCCCCGAG GAGTCACCCT GCAGTGCAGC GGAAGAGGAC 
GACTCCGAGT GGCTTTGCGG AGCAGGTGTC CATGGCAAAC CCGCGCCTCC AGAGGGTCTT 
AATGGGGACA GAGTCATAGT ACACATTGAT CTGGATTGCT TTTACGCACA AGTGGAAATG 
GTTCGCAATC CAGAACTGAG ACACAAACCT TTAGGCATTC AGCAGAAATA TTTGGTTGTC 
ACTTGTAACT ATGAAGCGAG AAGACAAGGC GTTGAGAAGA TGATGTCAGT CAGTAAGGCA 
AAAGAGAAGT GCCCACAGTT GGTCCTGGTC TGTGGAGAGA ACTTGACCCC CTACAGGGAG 
ATGTCTTACA AGATTACAGA ATTATTGGAA GAGTTTAGTC CACTTGTAGA AAGGCTAGGA 
TTTGATGAGA ATTTTGTGGA CATCACCAAG ATGGTAGAGA ACAGACTGAA ACAAATGCAG 
AGCAGTGGAT TATCAGCAAT ATCTGTTTCT GGCCATATAT ACAATAACCA AACTCTACAG 
CTGAATGAAG TGAAACATGT CCGGCTGGCC ATTGGATCCC ACATCGCAGC AGAGATGAGA 
GCAGCACTGT ATAACAGAGT CAGCCTGACT GGTTGCGCAG GAATTGCCTT TAACAAGTTA 
CTGGCCAAAC TGGTGTCTGG AACTTTTAAA CCCAACCAGC AGACAGTACT GCTGCCTGAG 
AGCACTCAAA ACCTAATGGA AAGCCTAGCC TACCTGACGA AAGTGCCTGG AATTGGTTAC 
AAAACTGCAA AACGCCTTGA GTCACTAGGA CTTACAACAA TACGTGATCT TCAGGCTTTT 
CCACGCGAAC TGCTGGAAAA GGAGTTTGGT GTTTCAGCCG CGCAACGCAT TCATAAGTTC 
AGCTACGGAG AGGATGACTC CTGCGTAACC CACTCAGGAC CTCCTCAGGT ACAAAGCGCC 
CTAACGTTTT AAAGCATGCC TAAATTATAC CACTCACTTG GCTCTTTTAC ACATTTCAAA 
ATACACAAAG ATGGAAGGCT GCCCTATACA ATACGGCTAA CGATTCGTCG CTTTTCAGCA 
ACTAATAAAT GGGTCAATCG AGAGAGCCGA CAGTGCCCCG TTCCAAGCCA TCTTTTACAA 
AAACTGGGGA CGGGAAGCTA TGAAGTAATT GCTGCCCTGA CGGAAGTCCT AATGAAGCTG 
TTCCAAAAAA TGGTTGATGT GAAAATGCCC TTTCACCTCA CCCTGCTAAA TGTGTGTTTC 
TCCAATCTGA AAGCCACTTG CACGTCTACC AAAGGATCCA TTGGGTTCTA TCTAAGTCCA 
AAAAAATCAG CCAATCCTAG CTTAAGCCAG TCTAGTCAGA GGACTGAGTG TACATTCAAT 
GAAGACAAGA CGAAAAGCTC TCTGGACACT GGACACAGGA CAGATAGTGA AACAGATGCT 
ACCAATTCTA GCGTGACAAG CATAAGGTTT TCTCAAACTG CTCCAGTATT ATTGGAAGAC 
ATTCCTGAAC AGCATAAATC TCAACATCTC TCAGCATTTC ACCTTCCTGC TGGGATTGAT 
TTTCAAGTGT TCAGTCAGCT TCCACCAGAG ATCCAGAAGG AACTTGTTTC CAGTGAGGCG 
ACAGTAAGAT CTCAGTTCAA CAGCAGTCCA AAACAAGAGA AACAGGCGTT GCAGAAATCT 
GTTCCCAAGA GCGGGGTGTT CTTTTTGCAA AAACAAATTT CCAGAAGTTT TGGTCATTCC 
AGCCATGAAG GTAAACCTAC AAGCCCTACA CCACAGGGTA GCGACTCTGA AAGCTTGTTG 
ATTAGTCCCC ATAGCCAGCC TGAAGTGAAT GTACCAGGTG AAAGGGATGA GGTTCACGTG 
GCAAACTGTG AAAGCTGGCC TAATGCTGAG AACTCTGAAA GCACACTAAC TGAGGGACCT 
ACAGACCAAA AACCTCTTCA GCTACTGCTG TCAGATCCAA GCAATATAAA TAGGCTTCAA 
CCTAAATGGC AAGGACCTCG TTCTAACCAG AGTTCACAAG TTGTAGGCTC CGAAATTCCA 
TCTTTGAGTA CGGCCCCTGG GTTTGCACAG CTGGTGGGAC CAATGGACCA GGAGCTCACA 
GGTCCATCCG TTCCGTTTCC CTCCCATGTT GACCCTGCCA CGTTCTCAGA GCTCCCTGCG 
GAGCTACAGG AGGAGCTGCT GGCGGAGTGG AAACAGCAGA AACCCGTTTC AAAAACTCAC 
AGTAGCACAC ACCTTGGGAA GGTGAAAGCC AAGAAGGGAA CCAAACGCAC TTTATCAAAG 
CCATCTCAGT CAAACAGTTT AATGAGGGCA CCAGCCTGGC ACGGCTTTAC CACTGCATCG 
GTGCTACTGC TTGTTCCAAC AGCAGGTGGT ACTGTTATAG CAAGGGGAGT CAGTCCCTAC 
CCCCCCACCA CCACCACCCC AAGACTGAGT GCACAGCCTC TGCCATGCTG CTTAGTGTTT 
GCTGGCCAGG ACCTGGAAGA TCCACCTCTG GATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005994715.2
CDS	175..2670
Translation	MEFLEPGSPEESPCSAAEEDDSEWLCGAGVHGKPAPPEGLNGDRVIVHIDLDCFYAQVEMVRNPELRHKPLGIQQKYLVVTCNYEARRQGVEKMMSVSKAKEKCPQLVLVCGENLTPYREMSYKITELLEEFSPLVERLGFDENFVDITKMVENRLKQMQSSGLSAISVSGHIYNNQTLQLNEVKHVRLAIGSHIAAEMRAALYNRVSLTGCAGIAFNKLLAKLVSGTFKPNQQTVLLPESTQNLMESLAYLTKVPGIGYKTAKRLESLGLTTIRDLQAFPRELLEKEFGVSAAQRIHKFSYGEDDSCVTHSGPPQVQSALTF*SMPKLYHSLGSFTHFKIHKDGRLPYTIRLTIRRFSATNKWVNRESRQCPVPSHLLQKLGTGSYEVIAALTEVLMKLFQKMVDVKMPFHLTLLNVCFSNLKATCTSTKGSIGFYLSPKKSANPSLSQSSQRTECTFNEDKTKSSLDTGHRTDSETDATNSSVTSIRFSQTAPVLLEDIPEQHKSQHLSAFHLPAGIDFQVFSQLPPEIQKELVSSEATVRSQFNSSPKQEKQALQKSVPKSGVFFLQKQISRSFGHSSHEGKPTSPTPQGSDSESLLISPHSQPEVNVPGERDEVHVANCESWPNAENSESTLTEGPTDQKPLQLLLSDPSNINRLQPKWQGPRSNQSSQVVGSEIPSLSTAPGFAQLVGPMDQELTGPSVPFPSHVDPATFSELPAELQEELLAEWKQQKPVSKTHSSTHLGKVKAKKGTKRTLSKPSQSNSLMRAPAWHGFTTASVLLLVPTAGGTVIARGVSPYPPTTTTPRLSAQPLPCCLVFAGQDLEDPPLD

Target ORF information:

RefSeq Version	XM_005994653.2
Organism	Latimeria chalumnae(coelacanth)
Definition	Latimeria chalumnae polymerase (DNA directed) iota (POLI), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005994653.2

ORF Insert Sequence:

ATGGAATTCC TAGAGCCCGG GAGCCCCGAG GAGTCACCCT GCAGTGCAGC GGAAGAGGAC 
GACTCCGAGT GGCTTTGCGG AGCAGGTGTC CATGGCAAAC CCGCGCCTCC AGAGGGTCTT 
AATGGGGACA GAGTCATAGT ACACATTGAT CTGGATTGCT TTTACGCACA AGTGGAAATG 
GTTCGCAATC CAGAACTGAG ACACAAACCT TTAGGCATTC AGCAGAAATA TTTGGTTGTC 
ACTTGTAACT ATGAAGCGAG AAGACAAGGC GTTGAGAAGA TGATGTCAGT CAGTAAGGCA 
AAAGAGAAGT GCCCACAGTT GGTCCTGGTC TGTGGAGAGA ACTTGACCCC CTACAGGGAG 
ATGTCTTACA AGATTACAGA ATTATTGGAA GAGTTTAGTC CACTTGTAGA AAGGCTAGGA 
TTTGATGAGA ATTTTGTGGA CATCACCAAG ATGGTAGAGA ACAGACTGAA ACAAATGCAG 
AGCAGTGGAT TATCAGCAAT ATCTGTTTCT GGCCATATAT ACAATAACCA AACTCTACAG 
CTGAATGAAG TGAAACATGT CCGGCTGGCC ATTGGATCCC ACATCGCAGC AGAGATGAGA 
GCAGCACTGT ATAACAGAGT CAGCCTGACT GGTTGCGCAG GAATTGCCTT TAACAAGTTA 
CTGGCCAAAC TGGTGTCTGG AACTTTTAAA CCCAACCAGC AGACAGTACT GCTGCCTGAG 
AGCACTCAAA ACCTAATGGA AAGCCTAGCC TACCTGACGA AAGTGCCTGG AATTGGTTAC 
AAAACTGCAA AACGCCTTGA GTCACTAGGA CTTACAACAA TACGTGATCT TCAGGCTTTT 
CCACGCGAAC TGCTGGAAAA GGAGTTTGGT GTTTCAGCCG CGCAACGCAT TCATAAGTTC 
AGCTACGGAG AGGATGACTC CTGCGTAACC CACTCAGGAC CTCCTCAGGT ACAAAGCGCC 
CTAACGTTTT AAAGCATGCC TAAATTATAC CACTCACTTG GCTCTTTTAC ACATTTCAAA 
ATACACAAAG ATGGAAGGCT GCCCTATACA ATACGGCTAA CGATTCGTCG CTTTTCAGCA 
ACTAATAAAT GGGTCAATCG AGAGAGCCGA CAGTGCCCCG TTCCAAGCCA TCTTTTACAA 
AAACTGGGGA CGGGAAGCTA TGAAGTAATT GCTGCCCTGA CGGAAGTCCT AATGAAGCTG 
TTCCAAAAAA TGGTTGATGT GAAAATGCCC TTTCACCTCA CCCTGCTAAA TGTGTGTTTC 
TCCAATCTGA AAGCCACTTG CACGTCTACC AAAGGATCCA TTGGGTTCTA TCTAAGTCCA 
AAAAAATCAG CCAATCCTAG CTTAAGCCAG TCTAGTCAGA GGACTGAGTG TACATTCAAT 
GAAGACAAGA CGAAAAGCTC TCTGGACACT GGACACAGGA CAGATAGTGA AACAGATGCT 
ACCAATTCTA GCGTGACAAG CATAAGGTTT TCTCAAACTG CTCCAGTATT ATTGGAAGAC 
ATTCCTGAAC AGCATAAATC TCAACATCTC TCAGCATTTC ACCTTCCTGC TGGGATTGAT 
TTTCAAGTGT TCAGTCAGCT TCCACCAGAG ATCCAGAAGG AACTTGTTTC CAGTGAGGCG 
ACAGTAAGAT CTCAGTTCAA CAGCAGTCCA AAACAAGAGA AACAGGCGTT GCAGAAATCT 
GTTCCCAAGA GCGGGGTGTT CTTTTTGCAA AAACAAATTT CCAGAAGTTT TGGTCATTCC 
AGCCATGAAG GTAAACCTAC AAGCCCTACA CCACAGGGTA GCGACTCTGA AAGCTTGTTG 
ATTAGTCCCC ATAGCCAGCC TGAAGTGAAT GTACCAGGTG AAAGGGATGA GGTTCACGTG 
GCAAACTGTG AAAGCTGGCC TAATGCTGAG AACTCTGAAA GCACACTAAC TGAGGGACCT 
ACAGACCAAA AACCTCTTCA GCTACTGCTG TCAGATCCAA GCAATATAAA TAGGCTTCAA 
CCTAAATGGC AAGGACCTCG TTCTAACCAG AGTTCACAAG TTGTAGGCTC CGAAATTCCA 
TCTTTGAGTA CGGCCCCTGG GTTTGCACAG CTGGTGGGAC CAATGGACCA GGAGCTCACA 
GGTCCATCCG TTCCGTTTCC CTCCCATGTT GACCCTGCCA CGTTCTCAGA GCTCCCTGCG 
GAGCTACAGG AGGAGCTGCT GGCGGAGTGG AAACAGCAGA AACCCGTTTC AAAAACTCAC 
AGTAGCACAC ACCTTGGGAA GGTGAAAGCC AAGAAGGGAA CCAAACGCAC TTTATCAAAG 
CCATCTCAGT CAAACAGTTT AATGAGGGCA CCAGCCTGGC ACGGCTTTAC CACTGCATCG 
GTGCTACTGC TTGTTCCAAC AGCAGGTGGT ACTGTTATAG CAAGGGGAGT CAGTCCCTAC 
CCCCCCACCA CCACCACCCC AAGACTGAGT GCACAGCCTC TGCCATGCTG CTTAGTGTTT 
GCTGGCCAGG ACCTGGAAGA TCCACCTCTG GATTGA