LOC104925646 cDNA ORF clone, Larimichthys crocea(large yellow croaker)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC104925646
Entrez Gene ID	104925646
Full Name	adenylate cyclase type 3
Synonyms	adcy3
Gene Type	protein-coding
Organism	Larimichthys crocea(large yellow croaker)

mRNA and Protein(s)

mRNA	Protein	Name
XM_027283874.1	XP_027139675.1	adenylate cyclase type 3
XM_019261442.1	XP_019116987.1	adenylate cyclase type 3

Pathways from BioSystems
Gene Ontology

The following LOC104925646 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC104925646 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OLa199692	XM_027283874.1 Latest version!	Larimichthys crocea adenylate cyclase type 3 (LOC104925646), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OLa92561	XM_019261442.1 Latest version!	Larimichthys crocea adenylate cyclase type 3 (LOC104925646), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC104925646 ( XM_027283874.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_027139675.1 Larimichthys crocea adenylate cyclase type 3 (LOC104925646), mRNA.

Price & Availability↑

CloneID	OLa199692
Clone ID Related Accession (Same CDS sequence)	XM_027283874.1
Accession Version	XM_027283874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-04
Organism	Larimichthys crocea(large yellow croaker)
Product	adenylate cyclase type 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_040021.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Larimichthys crocea Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCTGA ACCGGGTCCA CGCAACAGAC ACGGAGCAGT CTGCGGAGTA CTCAGTGGAG 
TACTCAGTGC AGGTACCCAG TGACTCCAGC CATGGAGCGG CTCGGACGCG TGAAGTGACC 
GTGCTCAAGT CGACCTGCTG CCGTTGCCTG CCGCGCGCAG TGCGCCTCAC TTTCACACCC 
GAATCACTGG AGAGACTTTA CCAGAGCTAC TTCCGACGAC AGAGACAGGA GAACCTGTTG 
GTGTTGGTGA TGTTCGCTGC TCTTTTCAAC AGCTTCATCA TCATCATGTG CGCGGTGGTG 
TACACAGAAG ACAAACTGGC CATGGTGGTG GTTGCAGCTG TGGGGCTGGC TGCGGACATT 
GTGCTGTACG TGCTGTGCTG GCTGCAGAAG CTCCCCACAT CACCGGTCTC ACGGGGAGCA 
GTGCCGTACG TCCTGTGGTT CATGGTCGCC GTGCACGTTT TATGTTACAT GGGACTGAAC 
TACGAGCGTT TCCCCCATGC CAGTGACTCT GTGGGCTGGC AGGCTTTTTT CAGTTTCTCC 
AGCTTTTTAA CTCTTCCACT GAACCTGGTG GCCCTGCTCC TGCTCACGGC CCTCTCATGT 
GGGATACACA CCCTCGTACT GGGGGTGACT GTGGCTCAAA GGTTTGATGA TAACCTGCAG 
GGGCCCATGC TGGTGAGACA GCTCCTAGCC AACGTGATGC TGTACCTGTG TGCAGCCATG 
GTGGGTGTCA TGTCATACTA CATGGAGGAC AGGAAGTACA GGACAGCTTT TTTGGAGGCT 
CGTCAGTCTC TAGAGGTCAA ACTCACCTTG GAGGAGCAGA GCACCCAGCA GGAGGAACTC 
TTACTGTCCA TCCTGCCCAA ACACATCGCT GATGAGATGT TGCAGGGCAT GAAGAACCAG 
GCCAATCAGC AAGATGTTCA GCAGCAGCAG CAGTTCAACA CCATGTACAT GTATCGTCAT 
GAAAACGTCA GTATCCTGTT TGCTGACATA GTGGGCTTCA CCCAGCTGTC CTCATCCTGT 
AGTGCCCAGG AGCTCGTAAA GCTACTTAAT GAACTGTTTG CCCGCTTCGA TAAACTGGCT 
GCAAAATATC ACCAACTGAG AATCAAGATC CTAGGAGACT GCTACTATTG CATCTGTGGT 
CTCCCTGACT TCAGAGACGA CCATGCAGCC TGCTCCATAA TGATGGGTCT AGCTATGGTG 
GAAGCCATCT CGTACGTGCG AGAGAAGACA AAAACCGAAG TGGACATGCG TGTGGGCGTT 
CACACGGGCA CCGTGCTGGG AGGAGTGCTC GGACAGAAGA GGTGGCAGTT TGATGTTTGG 
TCCACAGATG TTACTGTGGC CAATAAGATG GAGTCTGGAG GGATTCCTGG GAGGGTGCAT 
ATTTCCCAGA CCACTAAGGA CAGTCTGCAC GGAGAGTTTG AACTGGAGCC GGGGGATGGT 
GGAGAGAGGT GCGAGTACCT GCTGGAGAAA GGCATCAACA CTTACTTGGT TCTTGTGTCT 
AGTAAACAGA TGGCAAATGT ATTCAGCGGA AATAAACCAG GTACATTGTC CAACAGAAAT 
TCAAACCAGT TGATCAACAC TACAACAACC AATGGGAACG CAGCCTCACC CCAATCCACA 
CCCACTGAGT CTAAACAGGA GAGGAATAAG ATAGTGGAGG GACAAGTGAT CAACAGACGA 
CTGCAGCAGG AGCTACTAGA GAGAGAAACA CAGCAAATAA TGAAGGATCA CAAGATCAAC 
CCGCTGTCAT TGCGGTTTGT AGACTGGAAT TTGGAGGAGC ACTACTCCTC AGAGAAGGAG 
AAACGAAGCG GAGCGGCCTT CTGCTGCTGC ATCATAGTGC TCCTCTTCAT TACAGCAATG 
GAGGTGTTCA TAGACCCACT GTTGTATGTG AACTATGTGA CTCTCGCAAT CGGAGAGGTA 
TTGTTGCTTA TCCTCACACT GTGCTCCCTG GCTGCCATCT ACCCCAGGAT GTTCTCTAAG 
AGGTTGGTGT CTTTCTCAAT GTGGATTGAT CATACACGAT GGGCCAGAAA CACATGGGCC 
ATGGCGGCAA TATTTGTTCT CACCATGGCT GTGATCGCTG ACATGTTGAG CTGTGTCCCG 
CCGTCCCTTC GAGTCTTTAA CAGCACCTCA GGCCCCGTGT TGGAGTCATT CGGTGACCGA 
GGCTGCGCAG AGAATCCAAA ACACTACAGC TTCATGGCTG TGATGTCGCT CATCGCTGCC 
GCCATGTTGG TACAGGTCAG CCACCTGATC AAACTGGGCC TCATGGTGCT GGTAGTCACA 
GCAACTGGAG CTGTTAATAT CTATAGTTGG CGGGACATAT ATGATGTGTA TGACTATATA 
CAGTTTGCCT CATACAGAAC ATCCATAGTG CCATCCAAAT ACCTCATGAC CATGATGATC 
ATCGTTATGA TGATTGGCTT CTACTTCTTT GCCCGCCACT TGGAGCGTCA GTCCAGGAAG 
CTGTTCCTGT GGAAGATCGG TGTGCACGAC CAGAAGGAGA GGGTGTTTGA GATGAGACGC 
TGGAACGAAG CACTGGTCAC CAACATGCTG CCGGAGCATG TGGCCAAACA CTTCCTGGGC 
TCTAAGAAGA GAGATGAGGA GCTATACAGC CAGTCTTACA GTGAAATAGG CGTGATGTTT 
GCTTCCATCC CCAACTTTTC TGATTTCTAC ACTGAAGAGA GCATCAACAA CGGTGGCCTT 
GAGTGTCTCA GGATTCTCAA TGAGATAATC TCAGACTTTG ACAGCTTACT AGACAGAGAT 
GAGTTCCGCT GCATCACTAA GATTAAGACA ATAGGAAGCA CCTACATGGC CGCTTCAGGA 
CTCACTCCAG AGAGCAACAC AAACGGATAC AGCAACCTCA AGCCAGAGGA TCAGTCCCTG 
ATTGAGCGCT GGCAGCACCT CTCTGACCTG GCAGACTTTG CCTTGGCTAT GAAAGTCACC 
CTCAACAACC TCAACAAACA GTCTTTCAAC AACTTCATGC TCCGAATTGG TCTGAATAAG 
GGGGGAGTTT TGGCTGGAGT AATTGGAGCT CGTAAACCTC ACTATGACAT CTGGGGCAAC 
ACAGTCAATG TAGCCAGCAG AATGGAGTCA ACCGGAGTGA TGGGAAACAT CCAGGTGGTG 
GAAGACTGCT ATGACATCCT GAAGGACTAT GGCTTCCGCT TCATCCGGAG AGGGCCTATA 
TTTGTCAAAG GGAAAGGGGA GCTGCTAACC TTTTTCATGA AAGGAAAAGA CAAACCCAGT 
AGCAACGACG GTCCAGTGAC CACTGCCCTT CCACACCAAG TTGCAGACCT TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_027139675.1
CDS	189..3485
Translation	MSLNRVHATDTEQSAEYSVEYSVQVPSDSSHGAARTREVTVLKSTCCRCLPRAVRLTFTPESLERLYQSYFRRQRQENLLVLVMFAALFNSFIIIMCAVVYTEDKLAMVVVAAVGLAADIVLYVLCWLQKLPTSPVSRGAVPYVLWFMVAVHVLCYMGLNYERFPHASDSVGWQAFFSFSSFLTLPLNLVALLLLTALSCGIHTLVLGVTVAQRFDDNLQGPMLVRQLLANVMLYLCAAMVGVMSYYMEDRKYRTAFLEARQSLEVKLTLEEQSTQQEELLLSILPKHIADEMLQGMKNQANQQDVQQQQQFNTMYMYRHENVSILFADIVGFTQLSSSCSAQELVKLLNELFARFDKLAAKYHQLRIKILGDCYYCICGLPDFRDDHAACSIMMGLAMVEAISYVREKTKTEVDMRVGVHTGTVLGGVLGQKRWQFDVWSTDVTVANKMESGGIPGRVHISQTTKDSLHGEFELEPGDGGERCEYLLEKGINTYLVLVSSKQMANVFSGNKPGTLSNRNSNQLINTTTTNGNAASPQSTPTESKQERNKIVEGQVINRRLQQELLERETQQIMKDHKINPLSLRFVDWNLEEHYSSEKEKRSGAAFCCCIIVLLFITAMEVFIDPLLYVNYVTLAIGEVLLLILTLCSLAAIYPRMFSKRLVSFSMWIDHTRWARNTWAMAAIFVLTMAVIADMLSCVPPSLRVFNSTSGPVLESFGDRGCAENPKHYSFMAVMSLIAAAMLVQVSHLIKLGLMVLVVTATGAVNIYSWRDIYDVYDYIQFASYRTSIVPSKYLMTMMIIVMMIGFYFFARHLERQSRKLFLWKIGVHDQKERVFEMRRWNEALVTNMLPEHVAKHFLGSKKRDEELYSQSYSEIGVMFASIPNFSDFYTEESINNGGLECLRILNEIISDFDSLLDRDEFRCITKIKTIGSTYMAASGLTPESNTNGYSNLKPEDQSLIERWQHLSDLADFALAMKVTLNNLNKQSFNNFMLRIGLNKGGVLAGVIGARKPHYDIWGNTVNVASRMESTGVMGNIQVVEDCYDILKDYGFRFIRRGPIFVKGKGELLTFFMKGKDKPSSNDGPVTTALPHQVADLS

Target ORF information:

RefSeq Version	XM_027283874.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea adenylate cyclase type 3 (LOC104925646), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_027283874.1

ORF Insert Sequence:

ATGTCCCTGA ACCGGGTCCA CGCAACAGAC ACGGAGCAGT CTGCGGAGTA CTCAGTGGAG 
TACTCAGTGC AGGTACCCAG TGACTCCAGC CATGGAGCGG CTCGGACGCG TGAAGTGACC 
GTGCTCAAGT CGACCTGCTG CCGTTGCCTG CCGCGCGCAG TGCGCCTCAC TTTCACACCC 
GAATCACTGG AGAGACTTTA CCAGAGCTAC TTCCGACGAC AGAGACAGGA GAACCTGTTG 
GTGTTGGTGA TGTTCGCTGC TCTTTTCAAC AGCTTCATCA TCATCATGTG CGCGGTGGTG 
TACACAGAAG ACAAACTGGC CATGGTGGTG GTTGCAGCTG TGGGGCTGGC TGCGGACATT 
GTGCTGTACG TGCTGTGCTG GCTGCAGAAG CTCCCCACAT CACCGGTCTC ACGGGGAGCA 
GTGCCGTACG TCCTGTGGTT CATGGTCGCC GTGCACGTTT TATGTTACAT GGGACTGAAC 
TACGAGCGTT TCCCCCATGC CAGTGACTCT GTGGGCTGGC AGGCTTTTTT CAGTTTCTCC 
AGCTTTTTAA CTCTTCCACT GAACCTGGTG GCCCTGCTCC TGCTCACGGC CCTCTCATGT 
GGGATACACA CCCTCGTACT GGGGGTGACT GTGGCTCAAA GGTTTGATGA TAACCTGCAG 
GGGCCCATGC TGGTGAGACA GCTCCTAGCC AACGTGATGC TGTACCTGTG TGCAGCCATG 
GTGGGTGTCA TGTCATACTA CATGGAGGAC AGGAAGTACA GGACAGCTTT TTTGGAGGCT 
CGTCAGTCTC TAGAGGTCAA ACTCACCTTG GAGGAGCAGA GCACCCAGCA GGAGGAACTC 
TTACTGTCCA TCCTGCCCAA ACACATCGCT GATGAGATGT TGCAGGGCAT GAAGAACCAG 
GCCAATCAGC AAGATGTTCA GCAGCAGCAG CAGTTCAACA CCATGTACAT GTATCGTCAT 
GAAAACGTCA GTATCCTGTT TGCTGACATA GTGGGCTTCA CCCAGCTGTC CTCATCCTGT 
AGTGCCCAGG AGCTCGTAAA GCTACTTAAT GAACTGTTTG CCCGCTTCGA TAAACTGGCT 
GCAAAATATC ACCAACTGAG AATCAAGATC CTAGGAGACT GCTACTATTG CATCTGTGGT 
CTCCCTGACT TCAGAGACGA CCATGCAGCC TGCTCCATAA TGATGGGTCT AGCTATGGTG 
GAAGCCATCT CGTACGTGCG AGAGAAGACA AAAACCGAAG TGGACATGCG TGTGGGCGTT 
CACACGGGCA CCGTGCTGGG AGGAGTGCTC GGACAGAAGA GGTGGCAGTT TGATGTTTGG 
TCCACAGATG TTACTGTGGC CAATAAGATG GAGTCTGGAG GGATTCCTGG GAGGGTGCAT 
ATTTCCCAGA CCACTAAGGA CAGTCTGCAC GGAGAGTTTG AACTGGAGCC GGGGGATGGT 
GGAGAGAGGT GCGAGTACCT GCTGGAGAAA GGCATCAACA CTTACTTGGT TCTTGTGTCT 
AGTAAACAGA TGGCAAATGT ATTCAGCGGA AATAAACCAG GTACATTGTC CAACAGAAAT 
TCAAACCAGT TGATCAACAC TACAACAACC AATGGGAACG CAGCCTCACC CCAATCCACA 
CCCACTGAGT CTAAACAGGA GAGGAATAAG ATAGTGGAGG GACAAGTGAT CAACAGACGA 
CTGCAGCAGG AGCTACTAGA GAGAGAAACA CAGCAAATAA TGAAGGATCA CAAGATCAAC 
CCGCTGTCAT TGCGGTTTGT AGACTGGAAT TTGGAGGAGC ACTACTCCTC AGAGAAGGAG 
AAACGAAGCG GAGCGGCCTT CTGCTGCTGC ATCATAGTGC TCCTCTTCAT TACAGCAATG 
GAGGTGTTCA TAGACCCACT GTTGTATGTG AACTATGTGA CTCTCGCAAT CGGAGAGGTA 
TTGTTGCTTA TCCTCACACT GTGCTCCCTG GCTGCCATCT ACCCCAGGAT GTTCTCTAAG 
AGGTTGGTGT CTTTCTCAAT GTGGATTGAT CATACACGAT GGGCCAGAAA CACATGGGCC 
ATGGCGGCAA TATTTGTTCT CACCATGGCT GTGATCGCTG ACATGTTGAG CTGTGTCCCG 
CCGTCCCTTC GAGTCTTTAA CAGCACCTCA GGCCCCGTGT TGGAGTCATT CGGTGACCGA 
GGCTGCGCAG AGAATCCAAA ACACTACAGC TTCATGGCTG TGATGTCGCT CATCGCTGCC 
GCCATGTTGG TACAGGTCAG CCACCTGATC AAACTGGGCC TCATGGTGCT GGTAGTCACA 
GCAACTGGAG CTGTTAATAT CTATAGTTGG CGGGACATAT ATGATGTGTA TGACTATATA 
CAGTTTGCCT CATACAGAAC ATCCATAGTG CCATCCAAAT ACCTCATGAC CATGATGATC 
ATCGTTATGA TGATTGGCTT CTACTTCTTT GCCCGCCACT TGGAGCGTCA GTCCAGGAAG 
CTGTTCCTGT GGAAGATCGG TGTGCACGAC CAGAAGGAGA GGGTGTTTGA GATGAGACGC 
TGGAACGAAG CACTGGTCAC CAACATGCTG CCGGAGCATG TGGCCAAACA CTTCCTGGGC 
TCTAAGAAGA GAGATGAGGA GCTATACAGC CAGTCTTACA GTGAAATAGG CGTGATGTTT 
GCTTCCATCC CCAACTTTTC TGATTTCTAC ACTGAAGAGA GCATCAACAA CGGTGGCCTT 
GAGTGTCTCA GGATTCTCAA TGAGATAATC TCAGACTTTG ACAGCTTACT AGACAGAGAT 
GAGTTCCGCT GCATCACTAA GATTAAGACA ATAGGAAGCA CCTACATGGC CGCTTCAGGA 
CTCACTCCAG AGAGCAACAC AAACGGATAC AGCAACCTCA AGCCAGAGGA TCAGTCCCTG 
ATTGAGCGCT GGCAGCACCT CTCTGACCTG GCAGACTTTG CCTTGGCTAT GAAAGTCACC 
CTCAACAACC TCAACAAACA GTCTTTCAAC AACTTCATGC TCCGAATTGG TCTGAATAAG 
GGGGGAGTTT TGGCTGGAGT AATTGGAGCT CGTAAACCTC ACTATGACAT CTGGGGCAAC 
ACAGTCAATG TAGCCAGCAG AATGGAGTCA ACCGGAGTGA TGGGAAACAT CCAGGTGGTG 
GAAGACTGCT ATGACATCCT GAAGGACTAT GGCTTCCGCT TCATCCGGAG AGGGCCTATA 
TTTGTCAAAG GGAAAGGGGA GCTGCTAACC TTTTTCATGA AAGGAAAAGA CAAACCCAGT 
AGCAACGACG GTCCAGTGAC CACTGCCCTT CCACACCAAG TTGCAGACCT TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC104925646 ( XM_019261442.1 ) cDNA ORF clone, Larimichthys crocea(large yellow croaker) -> XP_019116987.1 Larimichthys crocea adenylate cyclase type 3 (LOC104925646), mRNA.

Price & Availability↑

CloneID	OLa92561
Clone ID Related Accession (Same CDS sequence)	XM_019261442.1
Accession Version	XM_019261442.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-11-28
Organism	Larimichthys crocea(large yellow croaker)
Product	adenylate cyclase type 3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_017608422.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Larimichthys crocea Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCTGA ACCGGGTCCA CGCAACAGAC ACGGAGCAGT CTGCGGAGTA CTCAGTGGAG 
TACTCAGTGC AGGTACCCAG TGACTCCAGC CATGGAGCGG CTCGGACGCG TGAAGTGACC 
GTGCTCAAGT CGACCTGCTG CCGTTGCCTG CCGCGCGCAG TGCGCCTCAC TTTCACACCC 
GAATCACTGG AGAGACTTTA CCAGAGCTAC TTCCGACGAC AGAGACAGGA GAACCTGTTG 
GTGTTGGTGA TGTTCGCTGC TCTTTTCAAC AGCTTCATCA TCATCATGTG CGCGGTGGTG 
TACACAGAAG ACAAACTGGC CATGGTGGTG GTTGCAGCTG TGGGGCTGGC TGCGGACATT 
GTGCTGTACG TGCTGTGCTG GCTGCAGAAG CTCCCCACAT CACCGGTCTC ACGGGGAGCA 
GTGCCGTACG TCCTGTGGTT CATGGTCGCC GTGCACGTTT TATGTTACAT GGGACTGAAC 
TACGAGCGTT TCCCCCATGC CAGTGACTCT GTGGGCTGGC AGGCTTTTTT CAGTTTCTCC 
AGCTTTTTAA CTCTTCCACT GAACCTGGTG GCCCTGCTCC TGCTCACGGC CCTCTCATGT 
GGGATACACA CCCTCGTACT GGGGGTGACT GTGGCTCAAA GGTTTGATGA TAACCTGCAG 
GGGCCCATGT TGGTGAGACA GCTCCTAGCC AACGTGATGC TGTACCTGTG CGCAGCCATG 
GTGGGTGTCA TGTCATACTA CATGGAGGAC AGGAAGTACA GGACAGCTTT TTTGGAGGCT 
CGTCAGTCAC TAGAGGTTAA ACTCACCTTG GAGGAGCAGA GCACCCAGCA GGAGGAACTC 
TTACTGTCCA TCCTGCCCAA ACACATCGCT GATGAGATGT TGCAGGGCAT GAAGAACCAG 
GCCAATCAGC AAGATGTTCA GCAGCAGCAG CAGTTCAACA CCATGTACAT GTATCGTCAT 
GAAAACGTCA GTATCCTGTT TGCTGACATA GTGGGCTTCA CCCAGCTGTC CTCATCCTGT 
AGTGCCCAGG AGCTCGTAAA GCTACTTAAT GAACTGTTTG CCCGCTTCGA TAAACTGGCT 
GCAAAATATC ACCAACTGAG AATCAAGATC CTAGGAGACT GCTACTATTG CATCTGTGGT 
CTCCCTGACT TCAGAGACGA CCATGCAGCC TGCTCCATAA TGATGGGTCT AGCTATGGTG 
GAAGCCATCT CGTACGTGCG AGAGAAGACA AAAACCGAAG TGGACATGCG TGTGGGCGTT 
CACACGGGCA CCGTGCTGGG AGGAGTGCTC GGACAGAAGA GGTGGCAGTT TGATGTTTGG 
TCCACAGATG TTACTGTGGC CAATAAGATG GAGTCTGGAG GGATTCCTGG GAGGGTGCAT 
ATTTCCCAGA CCACTAAGGA CAGTCTGCAC GGAGAGTTTG AACTGGAGCC GGGGGATGGT 
GGAGAGAGGT GCGAGTACCT GCTGGAGAAA GGCATCAACA CTTACTTGGT TCTTGTGTCT 
AGTAAACAGA TGGCAAATGT ATTCAGCGGA AATAAACCAG GTACATTGTC CAACAGAAAT 
TCAAACCAGT TGATCAACAC TACAACAACC AATGGGAACG CAGCCTCACC CCAATCCACA 
CCCACTGAGT CTAAACAGGA GAGGAATAAG ATAGTGGAGG GACAAGTGAT CAACAGACGA 
CTGCAGCAGG AGCTACTAGA GAGAGAAACA CAGCAAATAA TGAAGGATCA CAAGATCAAC 
CCGCTGTCAT TGCGGTTTGT AGACTGGAAT TTGGAGGAGC ACTACTCCTC AGAGAAGGAG 
AAACGAAGCG GAGCGGCCTT CTGCTGCTGC ATCATAGTGC TCCTCTTCAT TACAGCAATG 
GAGGTGTTCA TAGACCCACT GTTGTATGTG AACTATGTGA CTCTCGCAAT CGGAGAGGTA 
TTGTTGCTTA TCCTCACACT GTGCTCCCTG GCTGCCATCT ACCCCAGGAT GTTCTCCAAG 
AGGTTGGTGT CTTTCTCAAT GTGGATTGAT CATACACGAT GGGCCAGAAA CACATGGGCC 
ATGGCGGCAA TATTTGTTCT CACCATGGCT GTGATCGCTG ACATGTTGAG CTGTGTCCCG 
CCGTCCCTTC GAGTCTTTAA CAGCACCTCA GGCCCCGTGT TGGAGTCATT CGGTGACCGA 
GGCTGCGCAG AGAATCCAAA ACACTACAGC TTCATGGCTG TGATGTCGCT CATCGCTGCC 
GCCATGTTGG TACAGGTCAG CCACCTGATC AAACTGGGCC TCATGGTGCT GGTAGTCACA 
GCAACTGGAG CTGTTAATAT CTATAGTTGG CGGGACATAT ATGATGTGTA TGACTATATA 
CATTTCATTC ATGAACAACA GAAAGTGATG GTTGTATCAT CATTACCCTG CCTCTATCTT 
TGTTTTTCCA GAACATCCAT AGTGCCATCC AAATACCTCA TGACCATGAT GATCATCGTT 
ATGATGATTG GCTTCTACTT CTTTGCCCGC CACTTGGAGC GTCAGTCCAG GAAGCTGTTC 
CTGTGGAAGA TCGGTGTGCA CGACCAGAAG GAGAGGGTGT TTGAGATGAG ACGCTGGAAC 
GAAGCACTGG TCACCAACAT GCTGCCGGAG CATGTGGCCA AACACTTCCT GGGCTCTAAG 
AAGAGAGATG AGGAGCTATA CAGCCAGTCT TACAGTGAAA TAGGCGTGAT GTTTGCTTCC 
ATCCCCAACT TTTCTGATTT CTACACTGAA GAGAGCATCA ACAACGGTGG CCTTGAGTGT 
CTCAGGATTC TCAATGAGAT AATCTCAGAC TTTGACAGCT TACTAGACAG AGATGAGTTC 
CGCTGCATCA CTAAGATTAA GACAATAGGA AGCACCTACA TGGCCGCTTC AGGACTCACT 
CCAGAGAGCA ACACAAACGG ATACAGCAAC CTCAAGCCAG AGGATCAGTC CCTGATTGAG 
CGCTGGCAGC ACCTCTCTGA CCTGGCAGAC TTTGCCTTGG CTATGAAAGT CACCCTCAAC 
AACCTCAACA AACAGTCTTT CAACAACTTC ATGCTCCGAA TTGGTCTGAA TAAGGGGGGA 
GTTTTGGCTG GAGTAATTGG AGCTCGTAAA CCTCACTATG ACATCTGGGG CAACACAGTC 
AATGTAGCCA GCAGAATGGA GTCAACCGGA GTGATGGGAA ACATCCAGGT GGTGGAAGAC 
TGCTATGACA TCCTGAAGGA CTATGGCTTC CGCTTCATCC GGAGAGGGCC TATATTTGTC 
AAAGGGAAAG GGGAGCTGCT AACCTTTTTC ATGAAAGGAA AAGACAAACC CAGTAGCAAC 
GACGGTCCAG TGACCACTGC CCTTCCACAC CAAGTTGCAG ACCTTTCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_019116987.1
CDS	197..3547
Translation	MSLNRVHATDTEQSAEYSVEYSVQVPSDSSHGAARTREVTVLKSTCCRCLPRAVRLTFTPESLERLYQSYFRRQRQENLLVLVMFAALFNSFIIIMCAVVYTEDKLAMVVVAAVGLAADIVLYVLCWLQKLPTSPVSRGAVPYVLWFMVAVHVLCYMGLNYERFPHASDSVGWQAFFSFSSFLTLPLNLVALLLLTALSCGIHTLVLGVTVAQRFDDNLQGPMLVRQLLANVMLYLCAAMVGVMSYYMEDRKYRTAFLEARQSLEVKLTLEEQSTQQEELLLSILPKHIADEMLQGMKNQANQQDVQQQQQFNTMYMYRHENVSILFADIVGFTQLSSSCSAQELVKLLNELFARFDKLAAKYHQLRIKILGDCYYCICGLPDFRDDHAACSIMMGLAMVEAISYVREKTKTEVDMRVGVHTGTVLGGVLGQKRWQFDVWSTDVTVANKMESGGIPGRVHISQTTKDSLHGEFELEPGDGGERCEYLLEKGINTYLVLVSSKQMANVFSGNKPGTLSNRNSNQLINTTTTNGNAASPQSTPTESKQERNKIVEGQVINRRLQQELLERETQQIMKDHKINPLSLRFVDWNLEEHYSSEKEKRSGAAFCCCIIVLLFITAMEVFIDPLLYVNYVTLAIGEVLLLILTLCSLAAIYPRMFSKRLVSFSMWIDHTRWARNTWAMAAIFVLTMAVIADMLSCVPPSLRVFNSTSGPVLESFGDRGCAENPKHYSFMAVMSLIAAAMLVQVSHLIKLGLMVLVVTATGAVNIYSWRDIYDVYDYIHFIHEQQKVMVVSSLPCLYLCFSRTSIVPSKYLMTMMIIVMMIGFYFFARHLERQSRKLFLWKIGVHDQKERVFEMRRWNEALVTNMLPEHVAKHFLGSKKRDEELYSQSYSEIGVMFASIPNFSDFYTEESINNGGLECLRILNEIISDFDSLLDRDEFRCITKIKTIGSTYMAASGLTPESNTNGYSNLKPEDQSLIERWQHLSDLADFALAMKVTLNNLNKQSFNNFMLRIGLNKGGVLAGVIGARKPHYDIWGNTVNVASRMESTGVMGNIQVVEDCYDILKDYGFRFIRRGPIFVKGKGELLTFFMKGKDKPSSNDGPVTTALPHQVADLS

Target ORF information:

RefSeq Version	XM_019261442.1
Organism	Larimichthys crocea(large yellow croaker)
Definition	Larimichthys crocea adenylate cyclase type 3 (LOC104925646), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_019261442.1

ORF Insert Sequence:

ATGTCCCTGA ACCGGGTCCA CGCAACAGAC ACGGAGCAGT CTGCGGAGTA CTCAGTGGAG 
TACTCAGTGC AGGTACCCAG TGACTCCAGC CATGGAGCGG CTCGGACGCG TGAAGTGACC 
GTGCTCAAGT CGACCTGCTG CCGTTGCCTG CCGCGCGCAG TGCGCCTCAC TTTCACACCC 
GAATCACTGG AGAGACTTTA CCAGAGCTAC TTCCGACGAC AGAGACAGGA GAACCTGTTG 
GTGTTGGTGA TGTTCGCTGC TCTTTTCAAC AGCTTCATCA TCATCATGTG CGCGGTGGTG 
TACACAGAAG ACAAACTGGC CATGGTGGTG GTTGCAGCTG TGGGGCTGGC TGCGGACATT 
GTGCTGTACG TGCTGTGCTG GCTGCAGAAG CTCCCCACAT CACCGGTCTC ACGGGGAGCA 
GTGCCGTACG TCCTGTGGTT CATGGTCGCC GTGCACGTTT TATGTTACAT GGGACTGAAC 
TACGAGCGTT TCCCCCATGC CAGTGACTCT GTGGGCTGGC AGGCTTTTTT CAGTTTCTCC 
AGCTTTTTAA CTCTTCCACT GAACCTGGTG GCCCTGCTCC TGCTCACGGC CCTCTCATGT 
GGGATACACA CCCTCGTACT GGGGGTGACT GTGGCTCAAA GGTTTGATGA TAACCTGCAG 
GGGCCCATGT TGGTGAGACA GCTCCTAGCC AACGTGATGC TGTACCTGTG CGCAGCCATG 
GTGGGTGTCA TGTCATACTA CATGGAGGAC AGGAAGTACA GGACAGCTTT TTTGGAGGCT 
CGTCAGTCAC TAGAGGTTAA ACTCACCTTG GAGGAGCAGA GCACCCAGCA GGAGGAACTC 
TTACTGTCCA TCCTGCCCAA ACACATCGCT GATGAGATGT TGCAGGGCAT GAAGAACCAG 
GCCAATCAGC AAGATGTTCA GCAGCAGCAG CAGTTCAACA CCATGTACAT GTATCGTCAT 
GAAAACGTCA GTATCCTGTT TGCTGACATA GTGGGCTTCA CCCAGCTGTC CTCATCCTGT 
AGTGCCCAGG AGCTCGTAAA GCTACTTAAT GAACTGTTTG CCCGCTTCGA TAAACTGGCT 
GCAAAATATC ACCAACTGAG AATCAAGATC CTAGGAGACT GCTACTATTG CATCTGTGGT 
CTCCCTGACT TCAGAGACGA CCATGCAGCC TGCTCCATAA TGATGGGTCT AGCTATGGTG 
GAAGCCATCT CGTACGTGCG AGAGAAGACA AAAACCGAAG TGGACATGCG TGTGGGCGTT 
CACACGGGCA CCGTGCTGGG AGGAGTGCTC GGACAGAAGA GGTGGCAGTT TGATGTTTGG 
TCCACAGATG TTACTGTGGC CAATAAGATG GAGTCTGGAG GGATTCCTGG GAGGGTGCAT 
ATTTCCCAGA CCACTAAGGA CAGTCTGCAC GGAGAGTTTG AACTGGAGCC GGGGGATGGT 
GGAGAGAGGT GCGAGTACCT GCTGGAGAAA GGCATCAACA CTTACTTGGT TCTTGTGTCT 
AGTAAACAGA TGGCAAATGT ATTCAGCGGA AATAAACCAG GTACATTGTC CAACAGAAAT 
TCAAACCAGT TGATCAACAC TACAACAACC AATGGGAACG CAGCCTCACC CCAATCCACA 
CCCACTGAGT CTAAACAGGA GAGGAATAAG ATAGTGGAGG GACAAGTGAT CAACAGACGA 
CTGCAGCAGG AGCTACTAGA GAGAGAAACA CAGCAAATAA TGAAGGATCA CAAGATCAAC 
CCGCTGTCAT TGCGGTTTGT AGACTGGAAT TTGGAGGAGC ACTACTCCTC AGAGAAGGAG 
AAACGAAGCG GAGCGGCCTT CTGCTGCTGC ATCATAGTGC TCCTCTTCAT TACAGCAATG 
GAGGTGTTCA TAGACCCACT GTTGTATGTG AACTATGTGA CTCTCGCAAT CGGAGAGGTA 
TTGTTGCTTA TCCTCACACT GTGCTCCCTG GCTGCCATCT ACCCCAGGAT GTTCTCCAAG 
AGGTTGGTGT CTTTCTCAAT GTGGATTGAT CATACACGAT GGGCCAGAAA CACATGGGCC 
ATGGCGGCAA TATTTGTTCT CACCATGGCT GTGATCGCTG ACATGTTGAG CTGTGTCCCG 
CCGTCCCTTC GAGTCTTTAA CAGCACCTCA GGCCCCGTGT TGGAGTCATT CGGTGACCGA 
GGCTGCGCAG AGAATCCAAA ACACTACAGC TTCATGGCTG TGATGTCGCT CATCGCTGCC 
GCCATGTTGG TACAGGTCAG CCACCTGATC AAACTGGGCC TCATGGTGCT GGTAGTCACA 
GCAACTGGAG CTGTTAATAT CTATAGTTGG CGGGACATAT ATGATGTGTA TGACTATATA 
CATTTCATTC ATGAACAACA GAAAGTGATG GTTGTATCAT CATTACCCTG CCTCTATCTT 
TGTTTTTCCA GAACATCCAT AGTGCCATCC AAATACCTCA TGACCATGAT GATCATCGTT 
ATGATGATTG GCTTCTACTT CTTTGCCCGC CACTTGGAGC GTCAGTCCAG GAAGCTGTTC 
CTGTGGAAGA TCGGTGTGCA CGACCAGAAG GAGAGGGTGT TTGAGATGAG ACGCTGGAAC 
GAAGCACTGG TCACCAACAT GCTGCCGGAG CATGTGGCCA AACACTTCCT GGGCTCTAAG 
AAGAGAGATG AGGAGCTATA CAGCCAGTCT TACAGTGAAA TAGGCGTGAT GTTTGCTTCC 
ATCCCCAACT TTTCTGATTT CTACACTGAA GAGAGCATCA ACAACGGTGG CCTTGAGTGT 
CTCAGGATTC TCAATGAGAT AATCTCAGAC TTTGACAGCT TACTAGACAG AGATGAGTTC 
CGCTGCATCA CTAAGATTAA GACAATAGGA AGCACCTACA TGGCCGCTTC AGGACTCACT 
CCAGAGAGCA ACACAAACGG ATACAGCAAC CTCAAGCCAG AGGATCAGTC CCTGATTGAG 
CGCTGGCAGC ACCTCTCTGA CCTGGCAGAC TTTGCCTTGG CTATGAAAGT CACCCTCAAC 
AACCTCAACA AACAGTCTTT CAACAACTTC ATGCTCCGAA TTGGTCTGAA TAAGGGGGGA 
GTTTTGGCTG GAGTAATTGG AGCTCGTAAA CCTCACTATG ACATCTGGGG CAACACAGTC 
AATGTAGCCA GCAGAATGGA GTCAACCGGA GTGATGGGAA ACATCCAGGT GGTGGAAGAC 
TGCTATGACA TCCTGAAGGA CTATGGCTTC CGCTTCATCC GGAGAGGGCC TATATTTGTC 
AAAGGGAAAG GGGAGCTGCT AACCTTTTTC ATGAAAGGAA AAGACAAACC CAGTAGCAAC 
GACGGTCCAG TGACCACTGC CCTTCCACAC CAAGTTGCAG ACCTTTCTTG A