Nefl cDNA ORF clone, Jaculus jaculus(lesser Egyptian jerboa)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nefl
Entrez Gene ID	101617551
Full Name	neurofilament light
Gene Type	protein-coding
Organism	Jaculus jaculus(lesser Egyptian jerboa)

mRNA and Protein(s)

mRNA	Protein	Name
XM_004665820.2	XP_004665877.1	neurofilament light polypeptide

Pathways from BioSystems
Gene Ontology

The following Nefl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nefl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OJa97758	XM_004665820.2 Latest version!	Jaculus jaculus neurofilament, light polypeptide (Nefl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nefl ( XM_004665820.2 ) cDNA ORF clone, Jaculus jaculus(lesser Egyptian jerboa) -> XP_004665877.1 Jaculus jaculus neurofilament, light polypeptide (Nefl), mRNA.

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CloneID	OJa97758
Clone ID Related Accession (Same CDS sequence)	XM_004665820.2
Accession Version	XM_004665820.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1638bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-22
Organism	Jaculus jaculus(lesser Egyptian jerboa)
Product	neurofilament light polypeptide
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004504397.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_004665820.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Jaculus jaculus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCCT TCAGCTACGA GCCGTATTAT TCCACCTCGT ACAAGCGGCG CTACGTGGAG 
ACGCCCCGGG TGCACATCTC CAGCGTGCGC AGCGGCTACA GCTCGGCGCG CTCGGCGTAC 
TCCAGCTACT CGGCTCCCGT GTCGTCCTCG CTGTCCGTGC GCCGCAGCTA CTCGTCCAGC 
TCGAGTTCCT TGATGCCCAG CCTGGAGAAC CTCGATCTGA GCCAGGTGGC CGCCATCAGC 
AACGACCTCA AGTCCATCCG CACGCAGGAG AAGGCGCAGC TCCAGGACCT CAACGACCGC 
TTCGCCAGCT TCATCGAGCG CGTGCACGAG CTGGAGCAGC AGAACAAGGT CCTGGAAGCC 
GAGCTGCTCG TGCTGCGCCA GAAGCACTCG GAGCCGTCCC GCTTCCGGGC GCTGTACGAA 
CAGGAGATCC GTGACCTGCG CCTGGCTGCC GAGGACGCCA CCAACGAGAA GCAGGCGCTG 
CAGGGCGAGC GCGAGGGCCT GGAGGAGACG CTGCGCAGCC TGCAGGCGCG CTACGAGGAG 
GAGGTGCTGA GCCGCGAGGA CGCCGAGGGC CGCCTGATGG AGGCGCGCAA AGGCGCCGAC 
GAGGCCGCGC TGGCCCGCGC CGAGCTCGAG AAGCGCATCG ACAGCCTGAT GGACGAGATC 
GCCTTCCTGA AGAAAGTGCA CGAGGAGGAG ATCGCCGAGC TGCAGGCGCA GATCCAGTAC 
GCTCAGATCT CCGTGGAGAT GGACGTGTCC TCCAAGCCCG ACCTCTCCGC CGCGCTCAAG 
GACATCCGCG CCCAGTACGA GAAGCTGGCC GCCAAGAACA TGCAGAACGC CGAGGAGTGG 
TTCAAGAGCC GCTTCACCGT GCTCACCGAG AGCGCCGCCA AGAACACCGA CGCCGTGCGC 
GCCGCCAAGG ACGAGGTGTC CGAGAGCCGC CGCCTGCTGA AGGCCAAGAC CCTGGAGATC 
GAAGCCTGCC GGGGCATGAA CGAAGCGCTC GAGAAGCAGC TGCAGGAGCT GGAAGACAAG 
CAGAACGCTG ACATCAGTGC CATGCAGGAC ACAATCAACA AATTAGAAAA TGAGTTGAGG 
AGCACAAAGA GTGAGATGGC ACGGTACCTG AAGGAGTACC AGGACCTGCT CAACGTGAAG 
ATGGCTCTGG ATATTGAGAT TGCAGCCTAC AGGAAACTTT TGGAAGGCGA GGAGACACGG 
CTCAGTTTCA CCAGTGTGGG CAGCATAACC AGTGGCTACT CCCAGAGCTC CCAGGTCTTC 
GGCCGATCTG CCTACGGAGG TCTGCAGAGC AGCTCCTACC TGATGTCTGC TCGCTCCTTC 
CCTACGTACT ACACCAGCCA CGTCCAGGAG GAGCAGATCG AAGTGGAGGA GACCATCGAG 
GCAGCCAAGG CCGAGGAGGC CAAGGACGAG CCCCCCTCCG AAGGGGAAGC AGAGGAGGAG 
GAGAAGGAGA AGGAAGAGGG TGAGGAAGAG GAAGGAGGAG ATGAGGAAGA AGGTGCCAAG 
GAGGAATCCG AAGATGCAAA GGAGGAGGAA GAAGGAGGAG GGGAAGGCGA GGAAGGAGAG 
GAAACAAAAG AGTCAGAAGA GGATGAGAAG AAAGACGAAG GTGCTGTGGA GGAGCAAGCA 
GCCAAGAAGA AAGATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004665877.1
CDS	263..1900
Translation	MSSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSSARSAYSSYSAPVSSSLSVRRSYSSSSSSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRSLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEIAFLKKVHEEEIAELQAQIQYAQISVEMDVSSKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRSTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYGGLQSSSYLMSARSFPTYYTSHVQEEQIEVEETIEAAKAEEAKDEPPSEGEAEEEEKEKEEGEEEEGGDEEEGAKEESEDAKEEEEGGGEGEEGEETKESEEDEKKDEGAVEEQAAKKKD

Target ORF information:

RefSeq Version	XM_004665820.2
Organism	Jaculus jaculus(lesser Egyptian jerboa)
Definition	Jaculus jaculus neurofilament, light polypeptide (Nefl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004665820.2

ORF Insert Sequence:

ATGAGTTCCT TCAGCTACGA GCCGTATTAT TCCACCTCGT ACAAGCGGCG CTACGTGGAG 
ACGCCCCGGG TGCACATCTC CAGCGTGCGC AGCGGCTACA GCTCGGCGCG CTCGGCGTAC 
TCCAGCTACT CGGCTCCCGT GTCGTCCTCG CTGTCCGTGC GCCGCAGCTA CTCGTCCAGC 
TCGAGTTCCT TGATGCCCAG CCTGGAGAAC CTCGATCTGA GCCAGGTGGC CGCCATCAGC 
AACGACCTCA AGTCCATCCG CACGCAGGAG AAGGCGCAGC TCCAGGACCT CAACGACCGC 
TTCGCCAGCT TCATCGAGCG CGTGCACGAG CTGGAGCAGC AGAACAAGGT CCTGGAAGCC 
GAGCTGCTCG TGCTGCGCCA GAAGCACTCG GAGCCGTCCC GCTTCCGGGC GCTGTACGAA 
CAGGAGATCC GTGACCTGCG CCTGGCTGCC GAGGACGCCA CCAACGAGAA GCAGGCGCTG 
CAGGGCGAGC GCGAGGGCCT GGAGGAGACG CTGCGCAGCC TGCAGGCGCG CTACGAGGAG 
GAGGTGCTGA GCCGCGAGGA CGCCGAGGGC CGCCTGATGG AGGCGCGCAA AGGCGCCGAC 
GAGGCCGCGC TGGCCCGCGC CGAGCTCGAG AAGCGCATCG ACAGCCTGAT GGACGAGATC 
GCCTTCCTGA AGAAAGTGCA CGAGGAGGAG ATCGCCGAGC TGCAGGCGCA GATCCAGTAC 
GCTCAGATCT CCGTGGAGAT GGACGTGTCC TCCAAGCCCG ACCTCTCCGC CGCGCTCAAG 
GACATCCGCG CCCAGTACGA GAAGCTGGCC GCCAAGAACA TGCAGAACGC CGAGGAGTGG 
TTCAAGAGCC GCTTCACCGT GCTCACCGAG AGCGCCGCCA AGAACACCGA CGCCGTGCGC 
GCCGCCAAGG ACGAGGTGTC CGAGAGCCGC CGCCTGCTGA AGGCCAAGAC CCTGGAGATC 
GAAGCCTGCC GGGGCATGAA CGAAGCGCTC GAGAAGCAGC TGCAGGAGCT GGAAGACAAG 
CAGAACGCTG ACATCAGTGC CATGCAGGAC ACAATCAACA AATTAGAAAA TGAGTTGAGG 
AGCACAAAGA GTGAGATGGC ACGGTACCTG AAGGAGTACC AGGACCTGCT CAACGTGAAG 
ATGGCTCTGG ATATTGAGAT TGCAGCCTAC AGGAAACTTT TGGAAGGCGA GGAGACACGG 
CTCAGTTTCA CCAGTGTGGG CAGCATAACC AGTGGCTACT CCCAGAGCTC CCAGGTCTTC 
GGCCGATCTG CCTACGGAGG TCTGCAGAGC AGCTCCTACC TGATGTCTGC TCGCTCCTTC 
CCTACGTACT ACACCAGCCA CGTCCAGGAG GAGCAGATCG AAGTGGAGGA GACCATCGAG 
GCAGCCAAGG CCGAGGAGGC CAAGGACGAG CCCCCCTCCG AAGGGGAAGC AGAGGAGGAG 
GAGAAGGAGA AGGAAGAGGG TGAGGAAGAG GAAGGAGGAG ATGAGGAAGA AGGTGCCAAG 
GAGGAATCCG AAGATGCAAA GGAGGAGGAA GAAGGAGGAG GGGAAGGCGA GGAAGGAGAG 
GAAACAAAAG AGTCAGAAGA GGATGAGAAG AAAGACGAAG GTGCTGTGGA GGAGCAAGCA 
GCCAAGAAGA AAGATTGA