GPRASP1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GPRASP1
Entrez Gene ID	9737
Full Name	G protein-coupled receptor associated sorting protein 1
Synonyms	GASP,GASP-1,GASP1
General protein information	Preferred Names G protein-coupled receptor associated sorting protein 1 Names G-protein coupled receptor-associated sorting protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq22.1
Summary	This gene encodes a member of the GPRASP (G protein-coupled receptor associated sorting protein) family. The protein may modulate lysosomal sorting and functional down-regulation of a variety of G-protein coupled receptors. It targets receptors for degradation in lysosomes. The receptors interacting with this sorting protein include D2 dopamine receptor (DRD2), delta opioid receptor (OPRD1), beta-2 adrenergic receptor (ADRB2), D4 dopamine receptor (DRD4) and cannabinoid 1 receptor (CB1R). Multiple alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, May 2010].
Disorder MIM:	300417

mRNA and Protein(s)

mRNA	Protein	Name
NM_014710.5	NP_055525.3	G-protein coupled receptor-associated sorting protein 1
NM_001099411.1	NP_001092881.1	G-protein coupled receptor-associated sorting protein 1
NM_001184727.1	NP_001171656.1	G-protein coupled receptor-associated sorting protein 1
XM_017029981.1	XP_016885470.1	G-protein coupled receptor-associated sorting protein 1 isoform X1
XM_017029982.1	XP_016885471.1	G-protein coupled receptor-associated sorting protein 1 isoform X1
NM_014710.4	NP_055525.3	G-protein coupled receptor-associated sorting protein 1
NM_001184727.2	NP_001171656.1	G-protein coupled receptor-associated sorting protein 1
NM_001099410.1	NP_001092880.1	G-protein coupled receptor-associated sorting protein 1

Pathways from BioSystems

Homologies

	GPRASP1	XP_002806379.1
Canis lupus familiaris (dog)	GPRASP1	XP_005641653.1
Pan troglodytes (chimpanzee)	GPRASP1	XP_003317630.1
Homo sapiens (human)	GPRASP1	NP_055525.3
Mus musculus (house mouse)	Gprasp1	NP_001004359.1
Rattus norvegicus (Norway rat)	Gprasp1	NP_599213.1

Gene Ontology
Bibliography

Related articles in PubMed

Downregulation of 5-HT7 Serotonin Receptors by the Atypical Antipsychotics Clozapine and Olanzapine. Role of Motifs in the C-Terminal Domain and Interaction with GASP-1.
Manfra O, Van Craenenbroeck K, Skieterska K, Frimurer T, Schwartz TW, Levy FO, Andressen KW
ACS chemical neuroscience6(7)1206-18(2015 Jul)

Beclin 2 functions in autophagy, degradation of G protein-coupled receptors, and metabolism.
He C, Wei Y, Sun K, Li B, Dong X, Zou Z, Liu Y, Kinch LN, Khan S, Sinha S, Xavier RJ, Grishin NV, Xiao G, Eskelinen EL, Scherer PE, Whistler JL, Levine B
Cell154(5)1085-99(2013 Aug)

ARF6 and GASP-1 are post-endocytic sorting proteins selectively involved in the intracellular trafficking of dopamine D? receptors mediated by GRK and PKC in transfected cells.
Cho DI, Zheng M, Min C, Kwon KJ, Shin CY, Choi HK, Kim KM
British journal of pharmacology168(6)1355-74(2013 Mar)

G-protein coupled receptor-associated sorting protein 1 (GASP-1), a ubiquitous tumor marker.
Zheng X, Chang F, Zhang X, Rothman VL, Tuszynski GP
Experimental and molecular pathology93(1)111-5(2012 Aug)

Strength training with blood flow restriction diminishes myostatin gene expression.
Laurentino GC, Ugrinowitsch C, Roschel H, Aoki MS, Soares AG, Neves M Jr, Aihara AY, Fernandes Ada R, Tricoli V
Medicine and science in sports and exercise44(3)406-12(2012 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study demonstrated that binding of clozapine or olanzapine to the 5-HT7 receptor leads to antagonist-mediated lysosomal degradation by exposing key residues in the C-terminal tail that interact with GASP-1
Title: Downregulation of 5-HT7 Serotonin Receptors by the Atypical Antipsychotics Clozapine and Olanzapine. Role of Motifs in the C-Terminal Domain and Interaction with GASP-1.

Data suggest that specific serine/threonine residues in DRD2 (dopamine receptor D2) are involved in regulation of DRD2 endocytosis by GRK2 (G-protein coupled receptor kinase 2) and GASP1.
Title: ARF6 and GASP-1 are post-endocytic sorting proteins selectively involved in the intracellular trafficking of dopamine D? receptors mediated by GRK and PKC in transfected cells.

GASP1 was identified as a potential new serum and biomarker for brain, pancreatic, lung, and breast cancers, and gliomas.
Title: G-protein coupled receptor-associated sorting protein 1 (GASP-1), a ubiquitous tumor marker.

LIR was able to induce gains in 1RM and quadriceps CSA similar to those observed after traditional HI. These responses may be related to the concomitant decrease in MSTN and increase in FLST isoforms, GASP-1, and SMAD-7 mRNA gene expression.
Title: Strength training with blood flow restriction diminishes myostatin gene expression.

These studies identify GASP-1 as a potential new serum and tumor biomarker for breast cancer.
Title: G-protein coupled receptor-associated sorting protein 1 (GASP-1), a potential biomarker in breast cancer.

The following GPRASP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GPRASP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13433	NM_001099410.1 Latest version!	Homo sapiens G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu13433	NM_001184727.2 Latest version!	Homo sapiens G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu13433	NM_001099411.1 Latest version!	Homo sapiens G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu13433	NM_014710.5 Latest version!	Homo sapiens G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu13433	XM_017029981.1 Latest version!	Homo sapiens G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu13433	XM_017029982.1 Latest version!	Homo sapiens G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
View Old Accession Versions >>
OHu13433	NM_014710.4	Homo sapiens G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu13433	NM_001184727.1	Homo sapiens G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GPRASP1 ( NM_014710.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_055525.3 Homo sapiens G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant 1, mRNA.

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CloneID	OHu13433
Clone ID Related Accession (Same CDS sequence)	NM_014710.5 , NM_001099411.1 , NM_001184727.1 , XM_017029981.1 , XM_017029982.1 , NM_014710.4 , NM_001184727.2 , NM_001099410.1
Accession Version	NM_014710.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	G-protein coupled receptor-associated sorting protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA222893.1, BC114552.1, AY044233.1 and AW293629.1. On May 31, 2019 this sequence version replaced NM_014710.4. Transcript Variant: This variant (1) represents a dominant transcript. Variants 1-4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.105335.1, SRR1803615.139038.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTGGGG CAGAGATTGA GTCTGGTGCC CAGGTCAAGC CTGAAAAGAA GCCTGGGGAA 
GAGGTTGTAG GTGGGGCTGA GATAGAGAAT GATGTCCCTC TGGTGGTCAG ACCCAAGGTT 
AGGACCCAGG CCCAGATAAT GCCTGGGGCA AGGCCCAAGA ATAAGTCCAA GGTTATGCCT 
GGAGCAAGCA CCAAAGTTGA GACAAGTGCA GTGGGTGGGG CACGCCCTAA GAGTAAGGCC 
AAGGCAATAC CTGTTTCACG ATTTAAGGAA GAAGCCCAGA TGTGGGCTCA GCCCAGGTTT 
GGTGCTGAAA GATTGTCTAA GACAGAGAGA AACTCCCAGA CCAATATCAT AGCCTCTCCA 
CTTGTCAGTA CTGATTCTGT CTTGGTTGCT AAAACAAAGT ACCTGTCTGA GGATAGAGAA 
CTGGTTAATA CAGACACTGA GAGCTTTCCT AGAAGGAAGG CCCATTACCA AGCAGGATTC 
CAGCCTTCTT TTAGGTCAAA GGAGGAGACC AATATGGGGT CCTGGTGCTG TCCTAGGCCT 
ACATCCAAAC AAGAAGCCTC TCCTAATTCT GATTTCAAAT GGGTAGACAA ATCTGTGAGT 
TCCTTGTTCT GGAGTGGAGA TGAGGTCACT GCAAAATTTC ATCCTGGGAA TAGGGTAAAA 
GACAGTAACA GATCCATGCA CATGGCCAAT CAAGAGGCTA ATACCATGTC TAGGTCCCAA 
ACTAACCAGG AGCTCTATAT TGCATCTAGT TCTGGTTCTG AGGATGAGTC TGTTAAGACA 
CCCTGGTTCT GGGCCAGAGA TAAAACCAAT ACCTGGTCTG GGCCCAGGGA AGATCCCAAT 
AGCAGGTCCA GGTTTAGGTC TAAGAAAGAA GTCTATGTTG AATCAAGTTC TGGATCTGAG 
CATGAAGACC ATTTGGAGTC CTGGTTTGGG GCTGGAAAGG AGGCCAAATT CAGGTCCAAA 
ATGAGAGCTG GGAAGGAGGC CAATAACAGG GCCAGGCACA GGGCCAAGCG AGAAGCTTGC 
ATTGATTTCA TGCCTGGGTC TATAGATGTA ATTAAAAAAG AGTCCTGTTT CTGGCCTGAA 
GAAAATGCTA ATACCTTTTC AAGGCCCATG ATCAAGAAAG AGGCCAGGGC CAGAGCAATG 
ACAAAGGAAG AGGCCAAAAC CAAGGCCCGA GCCAGGGCCA AGCAAGAAGC CAGGTCAGAG 
GAGGAAGCCC TCATTGGGAC CTGGTTCTGG GCTACAGACG AGTCCAGCAT GGCAGATGAA 
GCCAGCATAG AGTCCAGTCT ACAAGTGGAG GATGAGTCCA TAATTGGGAG TTGGTTCTGG 
ACTGAAGAAG AGGCCAGTAT GGGGACTGGG GCTAGCAGTA AATCCAGACC AAGGACTGAT 
GGGGAGCGTA TTGGTGATTC CTTATTTGGG GCTAGGGAAA AGACCAGTAT GAAAACTGGG 
GCTGAGGCCA CCTCTGAATC TATACTAGCA GCTGATGATG AACAGGTCAT TATTGGTTCC 
TGGTTCTGGG CTGGTGAAGA GGTCAACCAA GAGGCTGAGG AAGAGACCAT TTTTGGGTCG 
TGGTTCTGGG TCATTGATGC GGCCAGTGTG GAATCTGGTG TTGGGGTCAG CTGTGAGTCC 
AGGACAAGGT CTGAGGAAGA AGAGGTCATT GGTCCCTGGT TTTGGTCTGG AGAACAAGTT 
GATATAGAGG CTGGAATCGG AGAAGAGGCC AGGCCAGGAG CTGAAGAAGA GACAATATTC 
GGGTCCTGGT TTTGGGCTGA AAACCAGACC TATATGGATT GTAGGGCTGA AACTAGCTGT 
GACACCATGC AAGGGGCTGA GGAGGAGGAG CCCATTATTG GGTCCTGGTT TTGGACCAGA 
GTAGAAGCTT GTGTGGAGGG TGATGTCAAC AGCAAGTCTA GCCTGGAGGA CAAGGAAGAG 
GCCATGATAC CATGTTTTGG AGCCAAAGAA GAGGTCAGTA TGAAGCATGG GACTGGTGTC 
AGATGCAGAT TTATGGCAGG GGCTGAGGAG ACCAATAATA AGTCTTGCTT CTGGGCAGAA 
AAAGAACCCT GTATGTATCC TGCCGGTGGA GGAAGTTGGA AGTCTAGGCC AGAGGAGGAA 
GAGGACATTG TCAATTCGTG GTTCTGGTCC AGAAAATACA CAAAGCCAGA GGCCATTATA 
GGGTCCTGGT TATGGGCTAC AGAAGAGAGT AATATAGATG GGACTGGAGA AAAGGCCAAG 
TTACTGACTG AAGAGGAGAC CATAATCAAT TCCTGGTTCT GGAAAGAAGA TGAAGCCATT 
TCAGAGGCTA CTGACAGAGA AGAGTCCAGG CCAGAAGCTG AGGAGGGGGA CATTATTGGT 
TCTTGGTTCT GGGCTGGAGA AGAGGACAGA CTAGAGCCAG CTGCTGAGAC TAGAGAAGAA 
GACAGGCTAG CAGCTGAGAA AGAAGGTATT GTTGGGTCCT GGTTTGGGGC CAGAGAAGAG 
ACCATTAGAA GAGAGGCTGG GTCTTGCAGC AAATCCAGTC CTAAAGCTGA AGAGGAAGAA 
GTCATTATTG GGTCCTGGTT CTGGGAAGAA GAGGCCAGTC CGGAGGCAGT GGCAGGAGTC 
GGCTTTGAGT CAAAGCCTGG GACTGAGGAG GAAGAAATCA CTGTTGGGTC CTGGTTCTGG 
CCTGAAGAAG AAGCCAGTAT ACAGGCTGGA TCTCAGGCAG TAGAGGAAAT GGAGTCAGAG 
ACTGAAGAGG AAACCATTTT TGGGTCCTGG TTCTGGGATG GAAAAGAAGT CAGTGAAGAA 
GCAGGACCAT GCTGTGTATC CAAGCCAGAG GATGATGAAG AGATGATTGT TGAGTCCTGG 
TTCTGGTCTA GAGACAAAGC CATTAAGGAA ACTGGAACTG TGGCCACCTG TGAGTCCAAG 
CCAGAAAATG AGGAAGGGGC CATTGTTGGG TCTTGGTTTG AGGCTGAAGA TGAGGTAGAT 
AACAGGACTG ACAATGGAAG CAACTGTGGG TCCAGGACAT TAGCTGATGA AGATGAGGCC 
ATAGTGGGGT CCTGGTTCTG GGCAGGAGAT GAGGCCCATT TTGAATCAAA TCCTAGCCCC 
GTGTTCAGGG CCATTTGCAG GTCCACGTGT TCAGTTGAAC AGGAGCCTGA TCCTTCACGC 
AGGCCTCAGA GTTGGGAGGA GGTCACTGTT CAGTTCAAGC CTGGTCCATG GGGTAGGGTC 
GGCTTCCCAT CTATAAGCCC CTTTAGATTT CCGAAAGAGG CAGCATCTTT ATTCTGTGAA 
ATGTTTGGGG GCAAACCCAG GAACATGGTA CTTAGCCCAG AAGGGGAAGA TCAGGAATCT 
TTGCTTCAGC CTGATCAGCC TAGTCCTGAG TTCCCATTTC AGTATGATCC TTCCTACAGG 
TCAGTCCAGG AAATTCGAGA GCATCTTAGG GCCAAGGAGA GTACAGAGCC TGAGAGTTCA 
TCCTGTAACT GCATACAATG TGAGCTGAAA ATTGGTTCTG AAGAGTTTGA AGAACTCCTT 
TTATTAATGG AAAAAATTCG GGATCCTTTT ATTCATGAAA TATCTAAAAT CGCAATGGGT 
ATGAGAAGTG CTTCTCAATT TACCCGAGAT TTCATTCGAG ATTCAGGTGT TGTCTCACTT 
ATTGAAACCT TGCTTAATTA TCCGTCCTCC CGAGTTAGAA CAAGTTTTTT GGAAAATATG 
ATTCGCATGG CCCCACCTTA TCCGAATCTA AACATAATTC AGACATACAT ATGTAAAGTG 
TGTGAGGAAA CCCTTGCTTA TAGCGTGGAT TCCCCGGAAC AGCTGTCTGG AATAAGGATG 
ATTAGACATC TCACTACTAC TACTGACTAT CACACACTGG TTGCCAATTA TATGTCTGGG 
TTTCTCTCCT TATTAGCTAC AGGCAATGCC AAAACAAGGT TTCATGTTTT GAAAATGCTA 
CTGAATTTGT CTGAAAATCT TTTCATGACA AAAGAACTAC TCAGTGCTGA AGCAGTGTCA 
GAATTTATAG GCCTCTTTAA CAGGGAAGAG ACAAATGACA ATATTCAAAT TGTTCTTGCA 
ATATTTGAGA ATATTGGCAA CAATATCAAA AAAGAAACAG TGTTCTCTGA TGATGATTTC 
AATATTGAGC CGCTTATTTC TGCATTCCAC AAAGTTGAGA AATTTGCTAA GGAACTGCAA 
GGCAAAACAG ACAATCAAAA TGACCCTGAA GGGGACCAAG AAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055525.3
CDS	685..4872
Translation	MTGAEIESGAQVKPEKKPGEEVVGGAEIENDVPLVVRPKVRTQAQIMPGARPKNKSKVMPGASTKVETSAVGGARPKSKAKAIPVSRFKEEAQMWAQPRFGAERLSKTERNSQTNIIASPLVSTDSVLVAKTKYLSEDRELVNTDTESFPRRKAHYQAGFQPSFRSKEETNMGSWCCPRPTSKQEASPNSDFKWVDKSVSSLFWSGDEVTAKFHPGNRVKDSNRSMHMANQEANTMSRSQTNQELYIASSSGSEDESVKTPWFWARDKTNTWSGPREDPNSRSRFRSKKEVYVESSSGSEHEDHLESWFGAGKEAKFRSKMRAGKEANNRARHRAKREACIDFMPGSIDVIKKESCFWPEENANTFSRPMIKKEARARAMTKEEAKTKARARAKQEARSEEEALIGTWFWATDESSMADEASIESSLQVEDESIIGSWFWTEEEASMGTGASSKSRPRTDGERIGDSLFGAREKTSMKTGAEATSESILAADDEQVIIGSWFWAGEEVNQEAEEETIFGSWFWVIDAASVESGVGVSCESRTRSEEEEVIGPWFWSGEQVDIEAGIGEEARPGAEEETIFGSWFWAENQTYMDCRAETSCDTMQGAEEEEPIIGSWFWTRVEACVEGDVNSKSSLEDKEEAMIPCFGAKEEVSMKHGTGVRCRFMAGAEETNNKSCFWAEKEPCMYPAGGGSWKSRPEEEEDIVNSWFWSRKYTKPEAIIGSWLWATEESNIDGTGEKAKLLTEEETIINSWFWKEDEAISEATDREESRPEAEEGDIIGSWFWAGEEDRLEPAAETREEDRLAAEKEGIVGSWFGAREETIRREAGSCSKSSPKAEEEEVIIGSWFWEEEASPEAVAGVGFESKPGTEEEEITVGSWFWPEEEASIQAGSQAVEEMESETEEETIFGSWFWDGKEVSEEAGPCCVSKPEDDEEMIVESWFWSRDKAIKETGTVATCESKPENEEGAIVGSWFEAEDEVDNRTDNGSNCGSRTLADEDEAIVGSWFWAGDEAHFESNPSPVFRAICRSTCSVEQEPDPSRRPQSWEEVTVQFKPGPWGRVGFPSISPFRFPKEAASLFCEMFGGKPRNMVLSPEGEDQESLLQPDQPSPEFPFQYDPSYRSVQEIREHLRAKESTEPESSSCNCIQCELKIGSEEFEELLLLMEKIRDPFIHEISKIAMGMRSASQFTRDFIRDSGVVSLIETLLNYPSSRVRTSFLENMIRMAPPYPNLNIIQTYICKVCEETLAYSVDSPEQLSGIRMIRHLTTTTDYHTLVANYMSGFLSLLATGNAKTRFHVLKMLLNLSENLFMTKELLSAEAVSEFIGLFNREETNDNIQIVLAIFENIGNNIKKETVFSDDDFNIEPLISAFHKVEKFAKELQGKTDNQNDPEGDQEN

Target ORF information:

RefSeq Version	NM_014710.5
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014710.5

ORF Insert Sequence:

ATGACTGGGG CAGAGATTGA GTCTGGTGCC CAGGTCAAGC CTGAAAAGAA GCCTGGGGAA 
GAGGTTGTAG GTGGGGCTGA GATAGAGAAT GATGTCCCTC TGGTGGTCAG ACCCAAGGTT 
AGGACCCAGG CCCAGATAAT GCCTGGGGCA AGGCCCAAGA ATAAGTCCAA GGTTATGCCT 
GGAGCAAGCA CCAAAGTTGA GACAAGTGCA GTGGGTGGGG CACGCCCTAA GAGTAAGGCC 
AAGGCAATAC CTGTTTCACG ATTTAAGGAA GAAGCCCAGA TGTGGGCTCA GCCCAGGTTT 
GGTGCTGAAA GATTGTCTAA GACAGAGAGA AACTCCCAGA CCAATATCAT AGCCTCTCCA 
CTTGTCAGTA CTGATTCTGT CTTGGTTGCT AAAACAAAGT ACCTGTCTGA GGATAGAGAA 
CTGGTTAATA CAGACACTGA GAGCTTTCCT AGAAGGAAGG CCCATTACCA AGCAGGATTC 
CAGCCTTCTT TTAGGTCAAA GGAGGAGACC AATATGGGGT CCTGGTGCTG TCCTAGGCCT 
ACATCCAAAC AAGAAGCCTC TCCTAATTCT GATTTCAAAT GGGTAGACAA ATCTGTGAGT 
TCCTTGTTCT GGAGTGGAGA TGAGGTCACT GCAAAATTTC ATCCTGGGAA TAGGGTAAAA 
GACAGTAACA GATCCATGCA CATGGCCAAT CAAGAGGCTA ATACCATGTC TAGGTCCCAA 
ACTAACCAGG AGCTCTATAT TGCATCTAGT TCTGGTTCTG AGGATGAGTC TGTTAAGACA 
CCCTGGTTCT GGGCCAGAGA TAAAACCAAT ACCTGGTCTG GGCCCAGGGA AGATCCCAAT 
AGCAGGTCCA GGTTTAGGTC TAAGAAAGAA GTCTATGTTG AATCAAGTTC TGGATCTGAG 
CATGAAGACC ATTTGGAGTC CTGGTTTGGG GCTGGAAAGG AGGCCAAATT CAGGTCCAAA 
ATGAGAGCTG GGAAGGAGGC CAATAACAGG GCCAGGCACA GGGCCAAGCG AGAAGCTTGC 
ATTGATTTCA TGCCTGGGTC TATAGATGTA ATTAAAAAAG AGTCCTGTTT CTGGCCTGAA 
GAAAATGCTA ATACCTTTTC AAGGCCCATG ATCAAGAAAG AGGCCAGGGC CAGAGCAATG 
ACAAAGGAAG AGGCCAAAAC CAAGGCCCGA GCCAGGGCCA AGCAAGAAGC CAGGTCAGAG 
GAGGAAGCCC TCATTGGGAC CTGGTTCTGG GCTACAGACG AGTCCAGCAT GGCAGATGAA 
GCCAGCATAG AGTCCAGTCT ACAAGTGGAG GATGAGTCCA TAATTGGGAG TTGGTTCTGG 
ACTGAAGAAG AGGCCAGTAT GGGGACTGGG GCTAGCAGTA AATCCAGACC AAGGACTGAT 
GGGGAGCGTA TTGGTGATTC CTTATTTGGG GCTAGGGAAA AGACCAGTAT GAAAACTGGG 
GCTGAGGCCA CCTCTGAATC TATACTAGCA GCTGATGATG AACAGGTCAT TATTGGTTCC 
TGGTTCTGGG CTGGTGAAGA GGTCAACCAA GAGGCTGAGG AAGAGACCAT TTTTGGGTCG 
TGGTTCTGGG TCATTGATGC GGCCAGTGTG GAATCTGGTG TTGGGGTCAG CTGTGAGTCC 
AGGACAAGGT CTGAGGAAGA AGAGGTCATT GGTCCCTGGT TTTGGTCTGG AGAACAAGTT 
GATATAGAGG CTGGAATCGG AGAAGAGGCC AGGCCAGGAG CTGAAGAAGA GACAATATTC 
GGGTCCTGGT TTTGGGCTGA AAACCAGACC TATATGGATT GTAGGGCTGA AACTAGCTGT 
GACACCATGC AAGGGGCTGA GGAGGAGGAG CCCATTATTG GGTCCTGGTT TTGGACCAGA 
GTAGAAGCTT GTGTGGAGGG TGATGTCAAC AGCAAGTCTA GCCTGGAGGA CAAGGAAGAG 
GCCATGATAC CATGTTTTGG AGCCAAAGAA GAGGTCAGTA TGAAGCATGG GACTGGTGTC 
AGATGCAGAT TTATGGCAGG GGCTGAGGAG ACCAATAATA AGTCTTGCTT CTGGGCAGAA 
AAAGAACCCT GTATGTATCC TGCCGGTGGA GGAAGTTGGA AGTCTAGGCC AGAGGAGGAA 
GAGGACATTG TCAATTCGTG GTTCTGGTCC AGAAAATACA CAAAGCCAGA GGCCATTATA 
GGGTCCTGGT TATGGGCTAC AGAAGAGAGT AATATAGATG GGACTGGAGA AAAGGCCAAG 
TTACTGACTG AAGAGGAGAC CATAATCAAT TCCTGGTTCT GGAAAGAAGA TGAAGCCATT 
TCAGAGGCTA CTGACAGAGA AGAGTCCAGG CCAGAAGCTG AGGAGGGGGA CATTATTGGT 
TCTTGGTTCT GGGCTGGAGA AGAGGACAGA CTAGAGCCAG CTGCTGAGAC TAGAGAAGAA 
GACAGGCTAG CAGCTGAGAA AGAAGGTATT GTTGGGTCCT GGTTTGGGGC CAGAGAAGAG 
ACCATTAGAA GAGAGGCTGG GTCTTGCAGC AAATCCAGTC CTAAAGCTGA AGAGGAAGAA 
GTCATTATTG GGTCCTGGTT CTGGGAAGAA GAGGCCAGTC CGGAGGCAGT GGCAGGAGTC 
GGCTTTGAGT CAAAGCCTGG GACTGAGGAG GAAGAAATCA CTGTTGGGTC CTGGTTCTGG 
CCTGAAGAAG AAGCCAGTAT ACAGGCTGGA TCTCAGGCAG TAGAGGAAAT GGAGTCAGAG 
ACTGAAGAGG AAACCATTTT TGGGTCCTGG TTCTGGGATG GAAAAGAAGT CAGTGAAGAA 
GCAGGACCAT GCTGTGTATC CAAGCCAGAG GATGATGAAG AGATGATTGT TGAGTCCTGG 
TTCTGGTCTA GAGACAAAGC CATTAAGGAA ACTGGAACTG TGGCCACCTG TGAGTCCAAG 
CCAGAAAATG AGGAAGGGGC CATTGTTGGG TCTTGGTTTG AGGCTGAAGA TGAGGTAGAT 
AACAGGACTG ACAATGGAAG CAACTGTGGG TCCAGGACAT TAGCTGATGA AGATGAGGCC 
ATAGTGGGGT CCTGGTTCTG GGCAGGAGAT GAGGCCCATT TTGAATCAAA TCCTAGCCCC 
GTGTTCAGGG CCATTTGCAG GTCCACGTGT TCAGTTGAAC AGGAGCCTGA TCCTTCACGC 
AGGCCTCAGA GTTGGGAGGA GGTCACTGTT CAGTTCAAGC CTGGTCCATG GGGTAGGGTC 
GGCTTCCCAT CTATAAGCCC CTTTAGATTT CCGAAAGAGG CAGCATCTTT ATTCTGTGAA 
ATGTTTGGGG GCAAACCCAG GAACATGGTA CTTAGCCCAG AAGGGGAAGA TCAGGAATCT 
TTGCTTCAGC CTGATCAGCC TAGTCCTGAG TTCCCATTTC AGTATGATCC TTCCTACAGG 
TCAGTCCAGG AAATTCGAGA GCATCTTAGG GCCAAGGAGA GTACAGAGCC TGAGAGTTCA 
TCCTGTAACT GCATACAATG TGAGCTGAAA ATTGGTTCTG AAGAGTTTGA AGAACTCCTT 
TTATTAATGG AAAAAATTCG GGATCCTTTT ATTCATGAAA TATCTAAAAT CGCAATGGGT 
ATGAGAAGTG CTTCTCAATT TACCCGAGAT TTCATTCGAG ATTCAGGTGT TGTCTCACTT 
ATTGAAACCT TGCTTAATTA TCCGTCCTCC CGAGTTAGAA CAAGTTTTTT GGAAAATATG 
ATTCGCATGG CCCCACCTTA TCCGAATCTA AACATAATTC AGACATACAT ATGTAAAGTG 
TGTGAGGAAA CCCTTGCTTA TAGCGTGGAT TCCCCGGAAC AGCTGTCTGG AATAAGGATG 
ATTAGACATC TCACTACTAC TACTGACTAT CACACACTGG TTGCCAATTA TATGTCTGGG 
TTTCTCTCCT TATTAGCTAC AGGCAATGCC AAAACAAGGT TTCATGTTTT GAAAATGCTA 
CTGAATTTGT CTGAAAATCT TTTCATGACA AAAGAACTAC TCAGTGCTGA AGCAGTGTCA 
GAATTTATAG GCCTCTTTAA CAGGGAAGAG ACAAATGACA ATATTCAAAT TGTTCTTGCA 
ATATTTGAGA ATATTGGCAA CAATATCAAA AAAGAAACAG TGTTCTCTGA TGATGATTTC 
AATATTGAGC CGCTTATTTC TGCATTCCAC AAAGTTGAGA AATTTGCTAA GGAACTGCAA 
GGCAAAACAG ACAATCAAAA TGACCCTGAA GGGGACCAAG AAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPRASP1 ( NM_001099411.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001092881.1 Homo sapiens G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant 3, mRNA.

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CloneID	OHu13433
Clone ID Related Accession (Same CDS sequence)	NM_014710.5 , NM_001099411.1 , NM_001184727.1 , XM_017029981.1 , XM_017029982.1 , NM_014710.4 , NM_001184727.2 , NM_001099410.1
Accession Version	NM_001099411.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	G-protein coupled receptor-associated sorting protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP193624.1, DA094297.1, BC114552.1, AY044233.1 and AW293629.1. Transcript Variant: This variant (3) lacks two exons in the 5' UTR and encodes the same protein, as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.89124.1, SRR1803612.39189.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTGGGG CAGAGATTGA GTCTGGTGCC CAGGTCAAGC CTGAAAAGAA GCCTGGGGAA 
GAGGTTGTAG GTGGGGCTGA GATAGAGAAT GATGTCCCTC TGGTGGTCAG ACCCAAGGTT 
AGGACCCAGG CCCAGATAAT GCCTGGGGCA AGGCCCAAGA ATAAGTCCAA GGTTATGCCT 
GGAGCAAGCA CCAAAGTTGA GACAAGTGCA GTGGGTGGGG CACGCCCTAA GAGTAAGGCC 
AAGGCAATAC CTGTTTCACG ATTTAAGGAA GAAGCCCAGA TGTGGGCTCA GCCCAGGTTT 
GGTGCTGAAA GATTGTCTAA GACAGAGAGA AACTCCCAGA CCAATATCAT AGCCTCTCCA 
CTTGTCAGTA CTGATTCTGT CTTGGTTGCT AAAACAAAGT ACCTGTCTGA GGATAGAGAA 
CTGGTTAATA CAGACACTGA GAGCTTTCCT AGAAGGAAGG CCCATTACCA AGCAGGATTC 
CAGCCTTCTT TTAGGTCAAA GGAGGAGACC AATATGGGGT CCTGGTGCTG TCCTAGGCCT 
ACATCCAAAC AAGAAGCCTC TCCTAATTCT GATTTCAAAT GGGTAGACAA ATCTGTGAGT 
TCCTTGTTCT GGAGTGGAGA TGAGGTCACT GCAAAATTTC ATCCTGGGAA TAGGGTAAAA 
GACAGTAACA GATCCATGCA CATGGCCAAT CAAGAGGCTA ATACCATGTC TAGGTCCCAA 
ACTAACCAGG AGCTCTATAT TGCATCTAGT TCTGGTTCTG AGGATGAGTC TGTTAAGACA 
CCCTGGTTCT GGGCCAGAGA TAAAACCAAT ACCTGGTCTG GGCCCAGGGA AGATCCCAAT 
AGCAGGTCCA GGTTTAGGTC TAAGAAAGAA GTCTATGTTG AATCAAGTTC TGGATCTGAG 
CATGAAGACC ATTTGGAGTC CTGGTTTGGG GCTGGAAAGG AGGCCAAATT CAGGTCCAAA 
ATGAGAGCTG GGAAGGAGGC CAATAACAGG GCCAGGCACA GGGCCAAGCG AGAAGCTTGC 
ATTGATTTCA TGCCTGGGTC TATAGATGTA ATTAAAAAAG AGTCCTGTTT CTGGCCTGAA 
GAAAATGCTA ATACCTTTTC AAGGCCCATG ATCAAGAAAG AGGCCAGGGC CAGAGCAATG 
ACAAAGGAAG AGGCCAAAAC CAAGGCCCGA GCCAGGGCCA AGCAAGAAGC CAGGTCAGAG 
GAGGAAGCCC TCATTGGGAC CTGGTTCTGG GCTACAGACG AGTCCAGCAT GGCAGATGAA 
GCCAGCATAG AGTCCAGTCT ACAAGTGGAG GATGAGTCCA TAATTGGGAG TTGGTTCTGG 
ACTGAAGAAG AGGCCAGTAT GGGGACTGGG GCTAGCAGTA AATCCAGACC AAGGACTGAT 
GGGGAGCGTA TTGGTGATTC CTTATTTGGG GCTAGGGAAA AGACCAGTAT GAAAACTGGG 
GCTGAGGCCA CCTCTGAATC TATACTAGCA GCTGATGATG AACAGGTCAT TATTGGTTCC 
TGGTTCTGGG CTGGTGAAGA GGTCAACCAA GAGGCTGAGG AAGAGACCAT TTTTGGGTCG 
TGGTTCTGGG TCATTGATGC GGCCAGTGTG GAATCTGGTG TTGGGGTCAG CTGTGAGTCC 
AGGACAAGGT CTGAGGAAGA AGAGGTCATT GGTCCCTGGT TTTGGTCTGG AGAACAAGTT 
GATATAGAGG CTGGAATCGG AGAAGAGGCC AGGCCAGGAG CTGAAGAAGA GACAATATTC 
GGGTCCTGGT TTTGGGCTGA AAACCAGACC TATATGGATT GTAGGGCTGA AACTAGCTGT 
GACACCATGC AAGGGGCTGA GGAGGAGGAG CCCATTATTG GGTCCTGGTT TTGGACCAGA 
GTAGAAGCTT GTGTGGAGGG TGATGTCAAC AGCAAGTCTA GCCTGGAGGA CAAGGAAGAG 
GCCATGATAC CATGTTTTGG AGCCAAAGAA GAGGTCAGTA TGAAGCATGG GACTGGTGTC 
AGATGCAGAT TTATGGCAGG GGCTGAGGAG ACCAATAATA AGTCTTGCTT CTGGGCAGAA 
AAAGAACCCT GTATGTATCC TGCCGGTGGA GGAAGTTGGA AGTCTAGGCC AGAGGAGGAA 
GAGGACATTG TCAATTCGTG GTTCTGGTCC AGAAAATACA CAAAGCCAGA GGCCATTATA 
GGGTCCTGGT TATGGGCTAC AGAAGAGAGT AATATAGATG GGACTGGAGA AAAGGCCAAG 
TTACTGACTG AAGAGGAGAC CATAATCAAT TCCTGGTTCT GGAAAGAAGA TGAAGCCATT 
TCAGAGGCTA CTGACAGAGA AGAGTCCAGG CCAGAAGCTG AGGAGGGGGA CATTATTGGT 
TCTTGGTTCT GGGCTGGAGA AGAGGACAGA CTAGAGCCAG CTGCTGAGAC TAGAGAAGAA 
GACAGGCTAG CAGCTGAGAA AGAAGGTATT GTTGGGTCCT GGTTTGGGGC CAGAGAAGAG 
ACCATTAGAA GAGAGGCTGG GTCTTGCAGC AAATCCAGTC CTAAAGCTGA AGAGGAAGAA 
GTCATTATTG GGTCCTGGTT CTGGGAAGAA GAGGCCAGTC CGGAGGCAGT GGCAGGAGTC 
GGCTTTGAGT CAAAGCCTGG GACTGAGGAG GAAGAAATCA CTGTTGGGTC CTGGTTCTGG 
CCTGAAGAAG AAGCCAGTAT ACAGGCTGGA TCTCAGGCAG TAGAGGAAAT GGAGTCAGAG 
ACTGAAGAGG AAACCATTTT TGGGTCCTGG TTCTGGGATG GAAAAGAAGT CAGTGAAGAA 
GCAGGACCAT GCTGTGTATC CAAGCCAGAG GATGATGAAG AGATGATTGT TGAGTCCTGG 
TTCTGGTCTA GAGACAAAGC CATTAAGGAA ACTGGAACTG TGGCCACCTG TGAGTCCAAG 
CCAGAAAATG AGGAAGGGGC CATTGTTGGG TCTTGGTTTG AGGCTGAAGA TGAGGTAGAT 
AACAGGACTG ACAATGGAAG CAACTGTGGG TCCAGGACAT TAGCTGATGA AGATGAGGCC 
ATAGTGGGGT CCTGGTTCTG GGCAGGAGAT GAGGCCCATT TTGAATCAAA TCCTAGCCCC 
GTGTTCAGGG CCATTTGCAG GTCCACGTGT TCAGTTGAAC AGGAGCCTGA TCCTTCACGC 
AGGCCTCAGA GTTGGGAGGA GGTCACTGTT CAGTTCAAGC CTGGTCCATG GGGTAGGGTC 
GGCTTCCCAT CTATAAGCCC CTTTAGATTT CCGAAAGAGG CAGCATCTTT ATTCTGTGAA 
ATGTTTGGGG GCAAACCCAG GAACATGGTA CTTAGCCCAG AAGGGGAAGA TCAGGAATCT 
TTGCTTCAGC CTGATCAGCC TAGTCCTGAG TTCCCATTTC AGTATGATCC TTCCTACAGG 
TCAGTCCAGG AAATTCGAGA GCATCTTAGG GCCAAGGAGA GTACAGAGCC TGAGAGTTCA 
TCCTGTAACT GCATACAATG TGAGCTGAAA ATTGGTTCTG AAGAGTTTGA AGAACTCCTT 
TTATTAATGG AAAAAATTCG GGATCCTTTT ATTCATGAAA TATCTAAAAT CGCAATGGGT 
ATGAGAAGTG CTTCTCAATT TACCCGAGAT TTCATTCGAG ATTCAGGTGT TGTCTCACTT 
ATTGAAACCT TGCTTAATTA TCCGTCCTCC CGAGTTAGAA CAAGTTTTTT GGAAAATATG 
ATTCGCATGG CCCCACCTTA TCCGAATCTA AACATAATTC AGACATACAT ATGTAAAGTG 
TGTGAGGAAA CCCTTGCTTA TAGCGTGGAT TCCCCGGAAC AGCTGTCTGG AATAAGGATG 
ATTAGACATC TCACTACTAC TACTGACTAT CACACACTGG TTGCCAATTA TATGTCTGGG 
TTTCTCTCCT TATTAGCTAC AGGCAATGCC AAAACAAGGT TTCATGTTTT GAAAATGCTA 
CTGAATTTGT CTGAAAATCT TTTCATGACA AAAGAACTAC TCAGTGCTGA AGCAGTGTCA 
GAATTTATAG GCCTCTTTAA CAGGGAAGAG ACAAATGACA ATATTCAAAT TGTTCTTGCA 
ATATTTGAGA ATATTGGCAA CAATATCAAA AAAGAAACAG TGTTCTCTGA TGATGATTTC 
AATATTGAGC CGCTTATTTC TGCATTCCAC AAAGTTGAGA AATTTGCTAA GGAACTGCAA 
GGCAAAACAG ACAATCAAAA TGACCCTGAA GGGGACCAAG AAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092881.1
CDS	643..4830
Translation	MTGAEIESGAQVKPEKKPGEEVVGGAEIENDVPLVVRPKVRTQAQIMPGARPKNKSKVMPGASTKVETSAVGGARPKSKAKAIPVSRFKEEAQMWAQPRFGAERLSKTERNSQTNIIASPLVSTDSVLVAKTKYLSEDRELVNTDTESFPRRKAHYQAGFQPSFRSKEETNMGSWCCPRPTSKQEASPNSDFKWVDKSVSSLFWSGDEVTAKFHPGNRVKDSNRSMHMANQEANTMSRSQTNQELYIASSSGSEDESVKTPWFWARDKTNTWSGPREDPNSRSRFRSKKEVYVESSSGSEHEDHLESWFGAGKEAKFRSKMRAGKEANNRARHRAKREACIDFMPGSIDVIKKESCFWPEENANTFSRPMIKKEARARAMTKEEAKTKARARAKQEARSEEEALIGTWFWATDESSMADEASIESSLQVEDESIIGSWFWTEEEASMGTGASSKSRPRTDGERIGDSLFGAREKTSMKTGAEATSESILAADDEQVIIGSWFWAGEEVNQEAEEETIFGSWFWVIDAASVESGVGVSCESRTRSEEEEVIGPWFWSGEQVDIEAGIGEEARPGAEEETIFGSWFWAENQTYMDCRAETSCDTMQGAEEEEPIIGSWFWTRVEACVEGDVNSKSSLEDKEEAMIPCFGAKEEVSMKHGTGVRCRFMAGAEETNNKSCFWAEKEPCMYPAGGGSWKSRPEEEEDIVNSWFWSRKYTKPEAIIGSWLWATEESNIDGTGEKAKLLTEEETIINSWFWKEDEAISEATDREESRPEAEEGDIIGSWFWAGEEDRLEPAAETREEDRLAAEKEGIVGSWFGAREETIRREAGSCSKSSPKAEEEEVIIGSWFWEEEASPEAVAGVGFESKPGTEEEEITVGSWFWPEEEASIQAGSQAVEEMESETEEETIFGSWFWDGKEVSEEAGPCCVSKPEDDEEMIVESWFWSRDKAIKETGTVATCESKPENEEGAIVGSWFEAEDEVDNRTDNGSNCGSRTLADEDEAIVGSWFWAGDEAHFESNPSPVFRAICRSTCSVEQEPDPSRRPQSWEEVTVQFKPGPWGRVGFPSISPFRFPKEAASLFCEMFGGKPRNMVLSPEGEDQESLLQPDQPSPEFPFQYDPSYRSVQEIREHLRAKESTEPESSSCNCIQCELKIGSEEFEELLLLMEKIRDPFIHEISKIAMGMRSASQFTRDFIRDSGVVSLIETLLNYPSSRVRTSFLENMIRMAPPYPNLNIIQTYICKVCEETLAYSVDSPEQLSGIRMIRHLTTTTDYHTLVANYMSGFLSLLATGNAKTRFHVLKMLLNLSENLFMTKELLSAEAVSEFIGLFNREETNDNIQIVLAIFENIGNNIKKETVFSDDDFNIEPLISAFHKVEKFAKELQGKTDNQNDPEGDQEN

Target ORF information:

RefSeq Version	NM_001099411.1
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001099411.1

ORF Insert Sequence:

ATGACTGGGG CAGAGATTGA GTCTGGTGCC CAGGTCAAGC CTGAAAAGAA GCCTGGGGAA 
GAGGTTGTAG GTGGGGCTGA GATAGAGAAT GATGTCCCTC TGGTGGTCAG ACCCAAGGTT 
AGGACCCAGG CCCAGATAAT GCCTGGGGCA AGGCCCAAGA ATAAGTCCAA GGTTATGCCT 
GGAGCAAGCA CCAAAGTTGA GACAAGTGCA GTGGGTGGGG CACGCCCTAA GAGTAAGGCC 
AAGGCAATAC CTGTTTCACG ATTTAAGGAA GAAGCCCAGA TGTGGGCTCA GCCCAGGTTT 
GGTGCTGAAA GATTGTCTAA GACAGAGAGA AACTCCCAGA CCAATATCAT AGCCTCTCCA 
CTTGTCAGTA CTGATTCTGT CTTGGTTGCT AAAACAAAGT ACCTGTCTGA GGATAGAGAA 
CTGGTTAATA CAGACACTGA GAGCTTTCCT AGAAGGAAGG CCCATTACCA AGCAGGATTC 
CAGCCTTCTT TTAGGTCAAA GGAGGAGACC AATATGGGGT CCTGGTGCTG TCCTAGGCCT 
ACATCCAAAC AAGAAGCCTC TCCTAATTCT GATTTCAAAT GGGTAGACAA ATCTGTGAGT 
TCCTTGTTCT GGAGTGGAGA TGAGGTCACT GCAAAATTTC ATCCTGGGAA TAGGGTAAAA 
GACAGTAACA GATCCATGCA CATGGCCAAT CAAGAGGCTA ATACCATGTC TAGGTCCCAA 
ACTAACCAGG AGCTCTATAT TGCATCTAGT TCTGGTTCTG AGGATGAGTC TGTTAAGACA 
CCCTGGTTCT GGGCCAGAGA TAAAACCAAT ACCTGGTCTG GGCCCAGGGA AGATCCCAAT 
AGCAGGTCCA GGTTTAGGTC TAAGAAAGAA GTCTATGTTG AATCAAGTTC TGGATCTGAG 
CATGAAGACC ATTTGGAGTC CTGGTTTGGG GCTGGAAAGG AGGCCAAATT CAGGTCCAAA 
ATGAGAGCTG GGAAGGAGGC CAATAACAGG GCCAGGCACA GGGCCAAGCG AGAAGCTTGC 
ATTGATTTCA TGCCTGGGTC TATAGATGTA ATTAAAAAAG AGTCCTGTTT CTGGCCTGAA 
GAAAATGCTA ATACCTTTTC AAGGCCCATG ATCAAGAAAG AGGCCAGGGC CAGAGCAATG 
ACAAAGGAAG AGGCCAAAAC CAAGGCCCGA GCCAGGGCCA AGCAAGAAGC CAGGTCAGAG 
GAGGAAGCCC TCATTGGGAC CTGGTTCTGG GCTACAGACG AGTCCAGCAT GGCAGATGAA 
GCCAGCATAG AGTCCAGTCT ACAAGTGGAG GATGAGTCCA TAATTGGGAG TTGGTTCTGG 
ACTGAAGAAG AGGCCAGTAT GGGGACTGGG GCTAGCAGTA AATCCAGACC AAGGACTGAT 
GGGGAGCGTA TTGGTGATTC CTTATTTGGG GCTAGGGAAA AGACCAGTAT GAAAACTGGG 
GCTGAGGCCA CCTCTGAATC TATACTAGCA GCTGATGATG AACAGGTCAT TATTGGTTCC 
TGGTTCTGGG CTGGTGAAGA GGTCAACCAA GAGGCTGAGG AAGAGACCAT TTTTGGGTCG 
TGGTTCTGGG TCATTGATGC GGCCAGTGTG GAATCTGGTG TTGGGGTCAG CTGTGAGTCC 
AGGACAAGGT CTGAGGAAGA AGAGGTCATT GGTCCCTGGT TTTGGTCTGG AGAACAAGTT 
GATATAGAGG CTGGAATCGG AGAAGAGGCC AGGCCAGGAG CTGAAGAAGA GACAATATTC 
GGGTCCTGGT TTTGGGCTGA AAACCAGACC TATATGGATT GTAGGGCTGA AACTAGCTGT 
GACACCATGC AAGGGGCTGA GGAGGAGGAG CCCATTATTG GGTCCTGGTT TTGGACCAGA 
GTAGAAGCTT GTGTGGAGGG TGATGTCAAC AGCAAGTCTA GCCTGGAGGA CAAGGAAGAG 
GCCATGATAC CATGTTTTGG AGCCAAAGAA GAGGTCAGTA TGAAGCATGG GACTGGTGTC 
AGATGCAGAT TTATGGCAGG GGCTGAGGAG ACCAATAATA AGTCTTGCTT CTGGGCAGAA 
AAAGAACCCT GTATGTATCC TGCCGGTGGA GGAAGTTGGA AGTCTAGGCC AGAGGAGGAA 
GAGGACATTG TCAATTCGTG GTTCTGGTCC AGAAAATACA CAAAGCCAGA GGCCATTATA 
GGGTCCTGGT TATGGGCTAC AGAAGAGAGT AATATAGATG GGACTGGAGA AAAGGCCAAG 
TTACTGACTG AAGAGGAGAC CATAATCAAT TCCTGGTTCT GGAAAGAAGA TGAAGCCATT 
TCAGAGGCTA CTGACAGAGA AGAGTCCAGG CCAGAAGCTG AGGAGGGGGA CATTATTGGT 
TCTTGGTTCT GGGCTGGAGA AGAGGACAGA CTAGAGCCAG CTGCTGAGAC TAGAGAAGAA 
GACAGGCTAG CAGCTGAGAA AGAAGGTATT GTTGGGTCCT GGTTTGGGGC CAGAGAAGAG 
ACCATTAGAA GAGAGGCTGG GTCTTGCAGC AAATCCAGTC CTAAAGCTGA AGAGGAAGAA 
GTCATTATTG GGTCCTGGTT CTGGGAAGAA GAGGCCAGTC CGGAGGCAGT GGCAGGAGTC 
GGCTTTGAGT CAAAGCCTGG GACTGAGGAG GAAGAAATCA CTGTTGGGTC CTGGTTCTGG 
CCTGAAGAAG AAGCCAGTAT ACAGGCTGGA TCTCAGGCAG TAGAGGAAAT GGAGTCAGAG 
ACTGAAGAGG AAACCATTTT TGGGTCCTGG TTCTGGGATG GAAAAGAAGT CAGTGAAGAA 
GCAGGACCAT GCTGTGTATC CAAGCCAGAG GATGATGAAG AGATGATTGT TGAGTCCTGG 
TTCTGGTCTA GAGACAAAGC CATTAAGGAA ACTGGAACTG TGGCCACCTG TGAGTCCAAG 
CCAGAAAATG AGGAAGGGGC CATTGTTGGG TCTTGGTTTG AGGCTGAAGA TGAGGTAGAT 
AACAGGACTG ACAATGGAAG CAACTGTGGG TCCAGGACAT TAGCTGATGA AGATGAGGCC 
ATAGTGGGGT CCTGGTTCTG GGCAGGAGAT GAGGCCCATT TTGAATCAAA TCCTAGCCCC 
GTGTTCAGGG CCATTTGCAG GTCCACGTGT TCAGTTGAAC AGGAGCCTGA TCCTTCACGC 
AGGCCTCAGA GTTGGGAGGA GGTCACTGTT CAGTTCAAGC CTGGTCCATG GGGTAGGGTC 
GGCTTCCCAT CTATAAGCCC CTTTAGATTT CCGAAAGAGG CAGCATCTTT ATTCTGTGAA 
ATGTTTGGGG GCAAACCCAG GAACATGGTA CTTAGCCCAG AAGGGGAAGA TCAGGAATCT 
TTGCTTCAGC CTGATCAGCC TAGTCCTGAG TTCCCATTTC AGTATGATCC TTCCTACAGG 
TCAGTCCAGG AAATTCGAGA GCATCTTAGG GCCAAGGAGA GTACAGAGCC TGAGAGTTCA 
TCCTGTAACT GCATACAATG TGAGCTGAAA ATTGGTTCTG AAGAGTTTGA AGAACTCCTT 
TTATTAATGG AAAAAATTCG GGATCCTTTT ATTCATGAAA TATCTAAAAT CGCAATGGGT 
ATGAGAAGTG CTTCTCAATT TACCCGAGAT TTCATTCGAG ATTCAGGTGT TGTCTCACTT 
ATTGAAACCT TGCTTAATTA TCCGTCCTCC CGAGTTAGAA CAAGTTTTTT GGAAAATATG 
ATTCGCATGG CCCCACCTTA TCCGAATCTA AACATAATTC AGACATACAT ATGTAAAGTG 
TGTGAGGAAA CCCTTGCTTA TAGCGTGGAT TCCCCGGAAC AGCTGTCTGG AATAAGGATG 
ATTAGACATC TCACTACTAC TACTGACTAT CACACACTGG TTGCCAATTA TATGTCTGGG 
TTTCTCTCCT TATTAGCTAC AGGCAATGCC AAAACAAGGT TTCATGTTTT GAAAATGCTA 
CTGAATTTGT CTGAAAATCT TTTCATGACA AAAGAACTAC TCAGTGCTGA AGCAGTGTCA 
GAATTTATAG GCCTCTTTAA CAGGGAAGAG ACAAATGACA ATATTCAAAT TGTTCTTGCA 
ATATTTGAGA ATATTGGCAA CAATATCAAA AAAGAAACAG TGTTCTCTGA TGATGATTTC 
AATATTGAGC CGCTTATTTC TGCATTCCAC AAAGTTGAGA AATTTGCTAA GGAACTGCAA 
GGCAAAACAG ACAATCAAAA TGACCCTGAA GGGGACCAAG AAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPRASP1 ( NM_001184727.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171656.1 Homo sapiens G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu13433
Clone ID Related Accession (Same CDS sequence)	NM_014710.5 , NM_001099411.1 , NM_001184727.1 , XM_017029981.1 , XM_017029982.1 , NM_014710.4 , NM_001184727.2 , NM_001099410.1
Accession Version	NM_001184727.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	G-protein coupled receptor-associated sorting protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX954816.1, BX489726.1, BC114552.1, AY044233.1 and AW293629.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (4) has an additional exon in the 5' UTR and encodes the same protein, as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.156277.1, SRR1803612.256013.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGACTGGGG CAGAGATTGA GTCTGGTGCC CAGGTCAAGC CTGAAAAGAA GCCTGGGGAA 
GAGGTTGTAG GTGGGGCTGA GATAGAGAAT GATGTCCCTC TGGTGGTCAG ACCCAAGGTT 
AGGACCCAGG CCCAGATAAT GCCTGGGGCA AGGCCCAAGA ATAAGTCCAA GGTTATGCCT 
GGAGCAAGCA CCAAAGTTGA GACAAGTGCA GTGGGTGGGG CACGCCCTAA GAGTAAGGCC 
AAGGCAATAC CTGTTTCACG ATTTAAGGAA GAAGCCCAGA TGTGGGCTCA GCCCAGGTTT 
GGTGCTGAAA GATTGTCTAA GACAGAGAGA AACTCCCAGA CCAATATCAT AGCCTCTCCA 
CTTGTCAGTA CTGATTCTGT CTTGGTTGCT AAAACAAAGT ACCTGTCTGA GGATAGAGAA 
CTGGTTAATA CAGACACTGA GAGCTTTCCT AGAAGGAAGG CCCATTACCA AGCAGGATTC 
CAGCCTTCTT TTAGGTCAAA GGAGGAGACC AATATGGGGT CCTGGTGCTG TCCTAGGCCT 
ACATCCAAAC AAGAAGCCTC TCCTAATTCT GATTTCAAAT GGGTAGACAA ATCTGTGAGT 
TCCTTGTTCT GGAGTGGAGA TGAGGTCACT GCAAAATTTC ATCCTGGGAA TAGGGTAAAA 
GACAGTAACA GATCCATGCA CATGGCCAAT CAAGAGGCTA ATACCATGTC TAGGTCCCAA 
ACTAACCAGG AGCTCTATAT TGCATCTAGT TCTGGTTCTG AGGATGAGTC TGTTAAGACA 
CCCTGGTTCT GGGCCAGAGA TAAAACCAAT ACCTGGTCTG GGCCCAGGGA AGATCCCAAT 
AGCAGGTCCA GGTTTAGGTC TAAGAAAGAA GTCTATGTTG AATCAAGTTC TGGATCTGAG 
CATGAAGACC ATTTGGAGTC CTGGTTTGGG GCTGGAAAGG AGGCCAAATT CAGGTCCAAA 
ATGAGAGCTG GGAAGGAGGC CAATAACAGG GCCAGGCACA GGGCCAAGCG AGAAGCTTGC 
ATTGATTTCA TGCCTGGGTC TATAGATGTA ATTAAAAAAG AGTCCTGTTT CTGGCCTGAA 
GAAAATGCTA ATACCTTTTC AAGGCCCATG ATCAAGAAAG AGGCCAGGGC CAGAGCAATG 
ACAAAGGAAG AGGCCAAAAC CAAGGCCCGA GCCAGGGCCA AGCAAGAAGC CAGGTCAGAG 
GAGGAAGCCC TCATTGGGAC CTGGTTCTGG GCTACAGACG AGTCCAGCAT GGCAGATGAA 
GCCAGCATAG AGTCCAGTCT ACAAGTGGAG GATGAGTCCA TAATTGGGAG TTGGTTCTGG 
ACTGAAGAAG AGGCCAGTAT GGGGACTGGG GCTAGCAGTA AATCCAGACC AAGGACTGAT 
GGGGAGCGTA TTGGTGATTC CTTATTTGGG GCTAGGGAAA AGACCAGTAT GAAAACTGGG 
GCTGAGGCCA CCTCTGAATC TATACTAGCA GCTGATGATG AACAGGTCAT TATTGGTTCC 
TGGTTCTGGG CTGGTGAAGA GGTCAACCAA GAGGCTGAGG AAGAGACCAT TTTTGGGTCG 
TGGTTCTGGG TCATTGATGC GGCCAGTGTG GAATCTGGTG TTGGGGTCAG CTGTGAGTCC 
AGGACAAGGT CTGAGGAAGA AGAGGTCATT GGTCCCTGGT TTTGGTCTGG AGAACAAGTT 
GATATAGAGG CTGGAATCGG AGAAGAGGCC AGGCCAGGAG CTGAAGAAGA GACAATATTC 
GGGTCCTGGT TTTGGGCTGA AAACCAGACC TATATGGATT GTAGGGCTGA AACTAGCTGT 
GACACCATGC AAGGGGCTGA GGAGGAGGAG CCCATTATTG GGTCCTGGTT TTGGACCAGA 
GTAGAAGCTT GTGTGGAGGG TGATGTCAAC AGCAAGTCTA GCCTGGAGGA CAAGGAAGAG 
GCCATGATAC CATGTTTTGG AGCCAAAGAA GAGGTCAGTA TGAAGCATGG GACTGGTGTC 
AGATGCAGAT TTATGGCAGG GGCTGAGGAG ACCAATAATA AGTCTTGCTT CTGGGCAGAA 
AAAGAACCCT GTATGTATCC TGCCGGTGGA GGAAGTTGGA AGTCTAGGCC AGAGGAGGAA 
GAGGACATTG TCAATTCGTG GTTCTGGTCC AGAAAATACA CAAAGCCAGA GGCCATTATA 
GGGTCCTGGT TATGGGCTAC AGAAGAGAGT AATATAGATG GGACTGGAGA AAAGGCCAAG 
TTACTGACTG AAGAGGAGAC CATAATCAAT TCCTGGTTCT GGAAAGAAGA TGAAGCCATT 
TCAGAGGCTA CTGACAGAGA AGAGTCCAGG CCAGAAGCTG AGGAGGGGGA CATTATTGGT 
TCTTGGTTCT GGGCTGGAGA AGAGGACAGA CTAGAGCCAG CTGCTGAGAC TAGAGAAGAA 
GACAGGCTAG CAGCTGAGAA AGAAGGTATT GTTGGGTCCT GGTTTGGGGC CAGAGAAGAG 
ACCATTAGAA GAGAGGCTGG GTCTTGCAGC AAATCCAGTC CTAAAGCTGA AGAGGAAGAA 
GTCATTATTG GGTCCTGGTT CTGGGAAGAA GAGGCCAGTC CGGAGGCAGT GGCAGGAGTC 
GGCTTTGAGT CAAAGCCTGG GACTGAGGAG GAAGAAATCA CTGTTGGGTC CTGGTTCTGG 
CCTGAAGAAG AAGCCAGTAT ACAGGCTGGA TCTCAGGCAG TAGAGGAAAT GGAGTCAGAG 
ACTGAAGAGG AAACCATTTT TGGGTCCTGG TTCTGGGATG GAAAAGAAGT CAGTGAAGAA 
GCAGGACCAT GCTGTGTATC CAAGCCAGAG GATGATGAAG AGATGATTGT TGAGTCCTGG 
TTCTGGTCTA GAGACAAAGC CATTAAGGAA ACTGGAACTG TGGCCACCTG TGAGTCCAAG 
CCAGAAAATG AGGAAGGGGC CATTGTTGGG TCTTGGTTTG AGGCTGAAGA TGAGGTAGAT 
AACAGGACTG ACAATGGAAG CAACTGTGGG TCCAGGACAT TAGCTGATGA AGATGAGGCC 
ATAGTGGGGT CCTGGTTCTG GGCAGGAGAT GAGGCCCATT TTGAATCAAA TCCTAGCCCC 
GTGTTCAGGG CCATTTGCAG GTCCACGTGT TCAGTTGAAC AGGAGCCTGA TCCTTCACGC 
AGGCCTCAGA GTTGGGAGGA GGTCACTGTT CAGTTCAAGC CTGGTCCATG GGGTAGGGTC 
GGCTTCCCAT CTATAAGCCC CTTTAGATTT CCGAAAGAGG CAGCATCTTT ATTCTGTGAA 
ATGTTTGGGG GCAAACCCAG GAACATGGTA CTTAGCCCAG AAGGGGAAGA TCAGGAATCT 
TTGCTTCAGC CTGATCAGCC TAGTCCTGAG TTCCCATTTC AGTATGATCC TTCCTACAGG 
TCAGTCCAGG AAATTCGAGA GCATCTTAGG GCCAAGGAGA GTACAGAGCC TGAGAGTTCA 
TCCTGTAACT GCATACAATG TGAGCTGAAA ATTGGTTCTG AAGAGTTTGA AGAACTCCTT 
TTATTAATGG AAAAAATTCG GGATCCTTTT ATTCATGAAA TATCTAAAAT CGCAATGGGT 
ATGAGAAGTG CTTCTCAATT TACCCGAGAT TTCATTCGAG ATTCAGGTGT TGTCTCACTT 
ATTGAAACCT TGCTTAATTA TCCGTCCTCC CGAGTTAGAA CAAGTTTTTT GGAAAATATG 
ATTCGCATGG CCCCACCTTA TCCGAATCTA AACATAATTC AGACATACAT ATGTAAAGTG 
TGTGAGGAAA CCCTTGCTTA TAGCGTGGAT TCCCCGGAAC AGCTGTCTGG AATAAGGATG 
ATTAGACATC TCACTACTAC TACTGACTAT CACACACTGG TTGCCAATTA TATGTCTGGG 
TTTCTCTCCT TATTAGCTAC AGGCAATGCC AAAACAAGGT TTCATGTTTT GAAAATGCTA 
CTGAATTTGT CTGAAAATCT TTTCATGACA AAAGAACTAC TCAGTGCTGA AGCAGTGTCA 
GAATTTATAG GCCTCTTTAA CAGGGAAGAG ACAAATGACA ATATTCAAAT TGTTCTTGCA 
ATATTTGAGA ATATTGGCAA CAATATCAAA AAAGAAACAG TGTTCTCTGA TGATGATTTC 
AATATTGAGC CGCTTATTTC TGCATTCCAC AAAGTTGAGA AATTTGCTAA GGAACTGCAA 
GGCAAAACAG ACAATCAAAA TGACCCTGAA GGGGACCAAG AAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171656.1
CDS	814..5001
Translation	MTGAEIESGAQVKPEKKPGEEVVGGAEIENDVPLVVRPKVRTQAQIMPGARPKNKSKVMPGASTKVETSAVGGARPKSKAKAIPVSRFKEEAQMWAQPRFGAERLSKTERNSQTNIIASPLVSTDSVLVAKTKYLSEDRELVNTDTESFPRRKAHYQAGFQPSFRSKEETNMGSWCCPRPTSKQEASPNSDFKWVDKSVSSLFWSGDEVTAKFHPGNRVKDSNRSMHMANQEANTMSRSQTNQELYIASSSGSEDESVKTPWFWARDKTNTWSGPREDPNSRSRFRSKKEVYVESSSGSEHEDHLESWFGAGKEAKFRSKMRAGKEANNRARHRAKREACIDFMPGSIDVIKKESCFWPEENANTFSRPMIKKEARARAMTKEEAKTKARARAKQEARSEEEALIGTWFWATDESSMADEASIESSLQVEDESIIGSWFWTEEEASMGTGASSKSRPRTDGERIGDSLFGAREKTSMKTGAEATSESILAADDEQVIIGSWFWAGEEVNQEAEEETIFGSWFWVIDAASVESGVGVSCESRTRSEEEEVIGPWFWSGEQVDIEAGIGEEARPGAEEETIFGSWFWAENQTYMDCRAETSCDTMQGAEEEEPIIGSWFWTRVEACVEGDVNSKSSLEDKEEAMIPCFGAKEEVSMKHGTGVRCRFMAGAEETNNKSCFWAEKEPCMYPAGGGSWKSRPEEEEDIVNSWFWSRKYTKPEAIIGSWLWATEESNIDGTGEKAKLLTEEETIINSWFWKEDEAISEATDREESRPEAEEGDIIGSWFWAGEEDRLEPAAETREEDRLAAEKEGIVGSWFGAREETIRREAGSCSKSSPKAEEEEVIIGSWFWEEEASPEAVAGVGFESKPGTEEEEITVGSWFWPEEEASIQAGSQAVEEMESETEEETIFGSWFWDGKEVSEEAGPCCVSKPEDDEEMIVESWFWSRDKAIKETGTVATCESKPENEEGAIVGSWFEAEDEVDNRTDNGSNCGSRTLADEDEAIVGSWFWAGDEAHFESNPSPVFRAICRSTCSVEQEPDPSRRPQSWEEVTVQFKPGPWGRVGFPSISPFRFPKEAASLFCEMFGGKPRNMVLSPEGEDQESLLQPDQPSPEFPFQYDPSYRSVQEIREHLRAKESTEPESSSCNCIQCELKIGSEEFEELLLLMEKIRDPFIHEISKIAMGMRSASQFTRDFIRDSGVVSLIETLLNYPSSRVRTSFLENMIRMAPPYPNLNIIQTYICKVCEETLAYSVDSPEQLSGIRMIRHLTTTTDYHTLVANYMSGFLSLLATGNAKTRFHVLKMLLNLSENLFMTKELLSAEAVSEFIGLFNREETNDNIQIVLAIFENIGNNIKKETVFSDDDFNIEPLISAFHKVEKFAKELQGKTDNQNDPEGDQEN

Target ORF information:

RefSeq Version	NM_001184727.1
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001184727.1

ORF Insert Sequence:

ATGACTGGGG CAGAGATTGA GTCTGGTGCC CAGGTCAAGC CTGAAAAGAA GCCTGGGGAA 
GAGGTTGTAG GTGGGGCTGA GATAGAGAAT GATGTCCCTC TGGTGGTCAG ACCCAAGGTT 
AGGACCCAGG CCCAGATAAT GCCTGGGGCA AGGCCCAAGA ATAAGTCCAA GGTTATGCCT 
GGAGCAAGCA CCAAAGTTGA GACAAGTGCA GTGGGTGGGG CACGCCCTAA GAGTAAGGCC 
AAGGCAATAC CTGTTTCACG ATTTAAGGAA GAAGCCCAGA TGTGGGCTCA GCCCAGGTTT 
GGTGCTGAAA GATTGTCTAA GACAGAGAGA AACTCCCAGA CCAATATCAT AGCCTCTCCA 
CTTGTCAGTA CTGATTCTGT CTTGGTTGCT AAAACAAAGT ACCTGTCTGA GGATAGAGAA 
CTGGTTAATA CAGACACTGA GAGCTTTCCT AGAAGGAAGG CCCATTACCA AGCAGGATTC 
CAGCCTTCTT TTAGGTCAAA GGAGGAGACC AATATGGGGT CCTGGTGCTG TCCTAGGCCT 
ACATCCAAAC AAGAAGCCTC TCCTAATTCT GATTTCAAAT GGGTAGACAA ATCTGTGAGT 
TCCTTGTTCT GGAGTGGAGA TGAGGTCACT GCAAAATTTC ATCCTGGGAA TAGGGTAAAA 
GACAGTAACA GATCCATGCA CATGGCCAAT CAAGAGGCTA ATACCATGTC TAGGTCCCAA 
ACTAACCAGG AGCTCTATAT TGCATCTAGT TCTGGTTCTG AGGATGAGTC TGTTAAGACA 
CCCTGGTTCT GGGCCAGAGA TAAAACCAAT ACCTGGTCTG GGCCCAGGGA AGATCCCAAT 
AGCAGGTCCA GGTTTAGGTC TAAGAAAGAA GTCTATGTTG AATCAAGTTC TGGATCTGAG 
CATGAAGACC ATTTGGAGTC CTGGTTTGGG GCTGGAAAGG AGGCCAAATT CAGGTCCAAA 
ATGAGAGCTG GGAAGGAGGC CAATAACAGG GCCAGGCACA GGGCCAAGCG AGAAGCTTGC 
ATTGATTTCA TGCCTGGGTC TATAGATGTA ATTAAAAAAG AGTCCTGTTT CTGGCCTGAA 
GAAAATGCTA ATACCTTTTC AAGGCCCATG ATCAAGAAAG AGGCCAGGGC CAGAGCAATG 
ACAAAGGAAG AGGCCAAAAC CAAGGCCCGA GCCAGGGCCA AGCAAGAAGC CAGGTCAGAG 
GAGGAAGCCC TCATTGGGAC CTGGTTCTGG GCTACAGACG AGTCCAGCAT GGCAGATGAA 
GCCAGCATAG AGTCCAGTCT ACAAGTGGAG GATGAGTCCA TAATTGGGAG TTGGTTCTGG 
ACTGAAGAAG AGGCCAGTAT GGGGACTGGG GCTAGCAGTA AATCCAGACC AAGGACTGAT 
GGGGAGCGTA TTGGTGATTC CTTATTTGGG GCTAGGGAAA AGACCAGTAT GAAAACTGGG 
GCTGAGGCCA CCTCTGAATC TATACTAGCA GCTGATGATG AACAGGTCAT TATTGGTTCC 
TGGTTCTGGG CTGGTGAAGA GGTCAACCAA GAGGCTGAGG AAGAGACCAT TTTTGGGTCG 
TGGTTCTGGG TCATTGATGC GGCCAGTGTG GAATCTGGTG TTGGGGTCAG CTGTGAGTCC 
AGGACAAGGT CTGAGGAAGA AGAGGTCATT GGTCCCTGGT TTTGGTCTGG AGAACAAGTT 
GATATAGAGG CTGGAATCGG AGAAGAGGCC AGGCCAGGAG CTGAAGAAGA GACAATATTC 
GGGTCCTGGT TTTGGGCTGA AAACCAGACC TATATGGATT GTAGGGCTGA AACTAGCTGT 
GACACCATGC AAGGGGCTGA GGAGGAGGAG CCCATTATTG GGTCCTGGTT TTGGACCAGA 
GTAGAAGCTT GTGTGGAGGG TGATGTCAAC AGCAAGTCTA GCCTGGAGGA CAAGGAAGAG 
GCCATGATAC CATGTTTTGG AGCCAAAGAA GAGGTCAGTA TGAAGCATGG GACTGGTGTC 
AGATGCAGAT TTATGGCAGG GGCTGAGGAG ACCAATAATA AGTCTTGCTT CTGGGCAGAA 
AAAGAACCCT GTATGTATCC TGCCGGTGGA GGAAGTTGGA AGTCTAGGCC AGAGGAGGAA 
GAGGACATTG TCAATTCGTG GTTCTGGTCC AGAAAATACA CAAAGCCAGA GGCCATTATA 
GGGTCCTGGT TATGGGCTAC AGAAGAGAGT AATATAGATG GGACTGGAGA AAAGGCCAAG 
TTACTGACTG AAGAGGAGAC CATAATCAAT TCCTGGTTCT GGAAAGAAGA TGAAGCCATT 
TCAGAGGCTA CTGACAGAGA AGAGTCCAGG CCAGAAGCTG AGGAGGGGGA CATTATTGGT 
TCTTGGTTCT GGGCTGGAGA AGAGGACAGA CTAGAGCCAG CTGCTGAGAC TAGAGAAGAA 
GACAGGCTAG CAGCTGAGAA AGAAGGTATT GTTGGGTCCT GGTTTGGGGC CAGAGAAGAG 
ACCATTAGAA GAGAGGCTGG GTCTTGCAGC AAATCCAGTC CTAAAGCTGA AGAGGAAGAA 
GTCATTATTG GGTCCTGGTT CTGGGAAGAA GAGGCCAGTC CGGAGGCAGT GGCAGGAGTC 
GGCTTTGAGT CAAAGCCTGG GACTGAGGAG GAAGAAATCA CTGTTGGGTC CTGGTTCTGG 
CCTGAAGAAG AAGCCAGTAT ACAGGCTGGA TCTCAGGCAG TAGAGGAAAT GGAGTCAGAG 
ACTGAAGAGG AAACCATTTT TGGGTCCTGG TTCTGGGATG GAAAAGAAGT CAGTGAAGAA 
GCAGGACCAT GCTGTGTATC CAAGCCAGAG GATGATGAAG AGATGATTGT TGAGTCCTGG 
TTCTGGTCTA GAGACAAAGC CATTAAGGAA ACTGGAACTG TGGCCACCTG TGAGTCCAAG 
CCAGAAAATG AGGAAGGGGC CATTGTTGGG TCTTGGTTTG AGGCTGAAGA TGAGGTAGAT 
AACAGGACTG ACAATGGAAG CAACTGTGGG TCCAGGACAT TAGCTGATGA AGATGAGGCC 
ATAGTGGGGT CCTGGTTCTG GGCAGGAGAT GAGGCCCATT TTGAATCAAA TCCTAGCCCC 
GTGTTCAGGG CCATTTGCAG GTCCACGTGT TCAGTTGAAC AGGAGCCTGA TCCTTCACGC 
AGGCCTCAGA GTTGGGAGGA GGTCACTGTT CAGTTCAAGC CTGGTCCATG GGGTAGGGTC 
GGCTTCCCAT CTATAAGCCC CTTTAGATTT CCGAAAGAGG CAGCATCTTT ATTCTGTGAA 
ATGTTTGGGG GCAAACCCAG GAACATGGTA CTTAGCCCAG AAGGGGAAGA TCAGGAATCT 
TTGCTTCAGC CTGATCAGCC TAGTCCTGAG TTCCCATTTC AGTATGATCC TTCCTACAGG 
TCAGTCCAGG AAATTCGAGA GCATCTTAGG GCCAAGGAGA GTACAGAGCC TGAGAGTTCA 
TCCTGTAACT GCATACAATG TGAGCTGAAA ATTGGTTCTG AAGAGTTTGA AGAACTCCTT 
TTATTAATGG AAAAAATTCG GGATCCTTTT ATTCATGAAA TATCTAAAAT CGCAATGGGT 
ATGAGAAGTG CTTCTCAATT TACCCGAGAT TTCATTCGAG ATTCAGGTGT TGTCTCACTT 
ATTGAAACCT TGCTTAATTA TCCGTCCTCC CGAGTTAGAA CAAGTTTTTT GGAAAATATG 
ATTCGCATGG CCCCACCTTA TCCGAATCTA AACATAATTC AGACATACAT ATGTAAAGTG 
TGTGAGGAAA CCCTTGCTTA TAGCGTGGAT TCCCCGGAAC AGCTGTCTGG AATAAGGATG 
ATTAGACATC TCACTACTAC TACTGACTAT CACACACTGG TTGCCAATTA TATGTCTGGG 
TTTCTCTCCT TATTAGCTAC AGGCAATGCC AAAACAAGGT TTCATGTTTT GAAAATGCTA 
CTGAATTTGT CTGAAAATCT TTTCATGACA AAAGAACTAC TCAGTGCTGA AGCAGTGTCA 
GAATTTATAG GCCTCTTTAA CAGGGAAGAG ACAAATGACA ATATTCAAAT TGTTCTTGCA 
ATATTTGAGA ATATTGGCAA CAATATCAAA AAAGAAACAG TGTTCTCTGA TGATGATTTC 
AATATTGAGC CGCTTATTTC TGCATTCCAC AAAGTTGAGA AATTTGCTAA GGAACTGCAA 
GGCAAAACAG ACAATCAAAA TGACCCTGAA GGGGACCAAG AAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPRASP1 ( NM_014710.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_055525.3 Homo sapiens G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant 1, mRNA.

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CloneID	OHu13433
Clone ID Related Accession (Same CDS sequence)	NM_014710.5 , NM_001099411.1 , NM_001184727.1 , XM_017029981.1 , XM_017029982.1 , NM_014710.4 , NM_001184727.2 , NM_001099410.1
Accession Version	NM_014710.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Homo sapiens(human)
Product	G-protein coupled receptor-associated sorting protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP193624.1, DA222893.1, BC114552.1, AY044233.1 and AW293629.1. On or before Aug 31, 2013 this sequence version replaced XM_005262231.1, NM_014710.3. Transcript Variant: This variant (1) represents a dominant transcript. Variants 1-4 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP193624.1, SRR1803615.105335.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTGGGG CAGAGATTGA GTCTGGTGCC CAGGTCAAGC CTGAAAAGAA GCCTGGGGAA 
GAGGTTGTAG GTGGGGCTGA GATAGAGAAT GATGTCCCTC TGGTGGTCAG ACCCAAGGTT 
AGGACCCAGG CCCAGATAAT GCCTGGGGCA AGGCCCAAGA ATAAGTCCAA GGTTATGCCT 
GGAGCAAGCA CCAAAGTTGA GACAAGTGCA GTGGGTGGGG CACGCCCTAA GAGTAAGGCC 
AAGGCAATAC CTGTTTCACG ATTTAAGGAA GAAGCCCAGA TGTGGGCTCA GCCCAGGTTT 
GGTGCTGAAA GATTGTCTAA GACAGAGAGA AACTCCCAGA CCAATATCAT AGCCTCTCCA 
CTTGTCAGTA CTGATTCTGT CTTGGTTGCT AAAACAAAGT ACCTGTCTGA GGATAGAGAA 
CTGGTTAATA CAGACACTGA GAGCTTTCCT AGAAGGAAGG CCCATTACCA AGCAGGATTC 
CAGCCTTCTT TTAGGTCAAA GGAGGAGACC AATATGGGGT CCTGGTGCTG TCCTAGGCCT 
ACATCCAAAC AAGAAGCCTC TCCTAATTCT GATTTCAAAT GGGTAGACAA ATCTGTGAGT 
TCCTTGTTCT GGAGTGGAGA TGAGGTCACT GCAAAATTTC ATCCTGGGAA TAGGGTAAAA 
GACAGTAACA GATCCATGCA CATGGCCAAT CAAGAGGCTA ATACCATGTC TAGGTCCCAA 
ACTAACCAGG AGCTCTATAT TGCATCTAGT TCTGGTTCTG AGGATGAGTC TGTTAAGACA 
CCCTGGTTCT GGGCCAGAGA TAAAACCAAT ACCTGGTCTG GGCCCAGGGA AGATCCCAAT 
AGCAGGTCCA GGTTTAGGTC TAAGAAAGAA GTCTATGTTG AATCAAGTTC TGGATCTGAG 
CATGAAGACC ATTTGGAGTC CTGGTTTGGG GCTGGAAAGG AGGCCAAATT CAGGTCCAAA 
ATGAGAGCTG GGAAGGAGGC CAATAACAGG GCCAGGCACA GGGCCAAGCG AGAAGCTTGC 
ATTGATTTCA TGCCTGGGTC TATAGATGTA ATTAAAAAAG AGTCCTGTTT CTGGCCTGAA 
GAAAATGCTA ATACCTTTTC AAGGCCCATG ATCAAGAAAG AGGCCAGGGC CAGAGCAATG 
ACAAAGGAAG AGGCCAAAAC CAAGGCCCGA GCCAGGGCCA AGCAAGAAGC CAGGTCAGAG 
GAGGAAGCCC TCATTGGGAC CTGGTTCTGG GCTACAGACG AGTCCAGCAT GGCAGATGAA 
GCCAGCATAG AGTCCAGTCT ACAAGTGGAG GATGAGTCCA TAATTGGGAG TTGGTTCTGG 
ACTGAAGAAG AGGCCAGTAT GGGGACTGGG GCTAGCAGTA AATCCAGACC AAGGACTGAT 
GGGGAGCGTA TTGGTGATTC CTTATTTGGG GCTAGGGAAA AGACCAGTAT GAAAACTGGG 
GCTGAGGCCA CCTCTGAATC TATACTAGCA GCTGATGATG AACAGGTCAT TATTGGTTCC 
TGGTTCTGGG CTGGTGAAGA GGTCAACCAA GAGGCTGAGG AAGAGACCAT TTTTGGGTCG 
TGGTTCTGGG TCATTGATGC GGCCAGTGTG GAATCTGGTG TTGGGGTCAG CTGTGAGTCC 
AGGACAAGGT CTGAGGAAGA AGAGGTCATT GGTCCCTGGT TTTGGTCTGG AGAACAAGTT 
GATATAGAGG CTGGAATCGG AGAAGAGGCC AGGCCAGGAG CTGAAGAAGA GACAATATTC 
GGGTCCTGGT TTTGGGCTGA AAACCAGACC TATATGGATT GTAGGGCTGA AACTAGCTGT 
GACACCATGC AAGGGGCTGA GGAGGAGGAG CCCATTATTG GGTCCTGGTT TTGGACCAGA 
GTAGAAGCTT GTGTGGAGGG TGATGTCAAC AGCAAGTCTA GCCTGGAGGA CAAGGAAGAG 
GCCATGATAC CATGTTTTGG AGCCAAAGAA GAGGTCAGTA TGAAGCATGG GACTGGTGTC 
AGATGCAGAT TTATGGCAGG GGCTGAGGAG ACCAATAATA AGTCTTGCTT CTGGGCAGAA 
AAAGAACCCT GTATGTATCC TGCCGGTGGA GGAAGTTGGA AGTCTAGGCC AGAGGAGGAA 
GAGGACATTG TCAATTCGTG GTTCTGGTCC AGAAAATACA CAAAGCCAGA GGCCATTATA 
GGGTCCTGGT TATGGGCTAC AGAAGAGAGT AATATAGATG GGACTGGAGA AAAGGCCAAG 
TTACTGACTG AAGAGGAGAC CATAATCAAT TCCTGGTTCT GGAAAGAAGA TGAAGCCATT 
TCAGAGGCTA CTGACAGAGA AGAGTCCAGG CCAGAAGCTG AGGAGGGGGA CATTATTGGT 
TCTTGGTTCT GGGCTGGAGA AGAGGACAGA CTAGAGCCAG CTGCTGAGAC TAGAGAAGAA 
GACAGGCTAG CAGCTGAGAA AGAAGGTATT GTTGGGTCCT GGTTTGGGGC CAGAGAAGAG 
ACCATTAGAA GAGAGGCTGG GTCTTGCAGC AAATCCAGTC CTAAAGCTGA AGAGGAAGAA 
GTCATTATTG GGTCCTGGTT CTGGGAAGAA GAGGCCAGTC CGGAGGCAGT GGCAGGAGTC 
GGCTTTGAGT CAAAGCCTGG GACTGAGGAG GAAGAAATCA CTGTTGGGTC CTGGTTCTGG 
CCTGAAGAAG AAGCCAGTAT ACAGGCTGGA TCTCAGGCAG TAGAGGAAAT GGAGTCAGAG 
ACTGAAGAGG AAACCATTTT TGGGTCCTGG TTCTGGGATG GAAAAGAAGT CAGTGAAGAA 
GCAGGACCAT GCTGTGTATC CAAGCCAGAG GATGATGAAG AGATGATTGT TGAGTCCTGG 
TTCTGGTCTA GAGACAAAGC CATTAAGGAA ACTGGAACTG TGGCCACCTG TGAGTCCAAG 
CCAGAAAATG AGGAAGGGGC CATTGTTGGG TCTTGGTTTG AGGCTGAAGA TGAGGTAGAT 
AACAGGACTG ACAATGGAAG CAACTGTGGG TCCAGGACAT TAGCTGATGA AGATGAGGCC 
ATAGTGGGGT CCTGGTTCTG GGCAGGAGAT GAGGCCCATT TTGAATCAAA TCCTAGCCCC 
GTGTTCAGGG CCATTTGCAG GTCCACGTGT TCAGTTGAAC AGGAGCCTGA TCCTTCACGC 
AGGCCTCAGA GTTGGGAGGA GGTCACTGTT CAGTTCAAGC CTGGTCCATG GGGTAGGGTC 
GGCTTCCCAT CTATAAGCCC CTTTAGATTT CCGAAAGAGG CAGCATCTTT ATTCTGTGAA 
ATGTTTGGGG GCAAACCCAG GAACATGGTA CTTAGCCCAG AAGGGGAAGA TCAGGAATCT 
TTGCTTCAGC CTGATCAGCC TAGTCCTGAG TTCCCATTTC AGTATGATCC TTCCTACAGG 
TCAGTCCAGG AAATTCGAGA GCATCTTAGG GCCAAGGAGA GTACAGAGCC TGAGAGTTCA 
TCCTGTAACT GCATACAATG TGAGCTGAAA ATTGGTTCTG AAGAGTTTGA AGAACTCCTT 
TTATTAATGG AAAAAATTCG GGATCCTTTT ATTCATGAAA TATCTAAAAT CGCAATGGGT 
ATGAGAAGTG CTTCTCAATT TACCCGAGAT TTCATTCGAG ATTCAGGTGT TGTCTCACTT 
ATTGAAACCT TGCTTAATTA TCCGTCCTCC CGAGTTAGAA CAAGTTTTTT GGAAAATATG 
ATTCGCATGG CCCCACCTTA TCCGAATCTA AACATAATTC AGACATACAT ATGTAAAGTG 
TGTGAGGAAA CCCTTGCTTA TAGCGTGGAT TCCCCGGAAC AGCTGTCTGG AATAAGGATG 
ATTAGACATC TCACTACTAC TACTGACTAT CACACACTGG TTGCCAATTA TATGTCTGGG 
TTTCTCTCCT TATTAGCTAC AGGCAATGCC AAAACAAGGT TTCATGTTTT GAAAATGCTA 
CTGAATTTGT CTGAAAATCT TTTCATGACA AAAGAACTAC TCAGTGCTGA AGCAGTGTCA 
GAATTTATAG GCCTCTTTAA CAGGGAAGAG ACAAATGACA ATATTCAAAT TGTTCTTGCA 
ATATTTGAGA ATATTGGCAA CAATATCAAA AAAGAAACAG TGTTCTCTGA TGATGATTTC 
AATATTGAGC CGCTTATTTC TGCATTCCAC AAAGTTGAGA AATTTGCTAA GGAACTGCAA 
GGCAAAACAG ACAATCAAAA TGACCCTGAA GGGGACCAAG AAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055525.3
CDS	802..4989
Translation	MTGAEIESGAQVKPEKKPGEEVVGGAEIENDVPLVVRPKVRTQAQIMPGARPKNKSKVMPGASTKVETSAVGGARPKSKAKAIPVSRFKEEAQMWAQPRFGAERLSKTERNSQTNIIASPLVSTDSVLVAKTKYLSEDRELVNTDTESFPRRKAHYQAGFQPSFRSKEETNMGSWCCPRPTSKQEASPNSDFKWVDKSVSSLFWSGDEVTAKFHPGNRVKDSNRSMHMANQEANTMSRSQTNQELYIASSSGSEDESVKTPWFWARDKTNTWSGPREDPNSRSRFRSKKEVYVESSSGSEHEDHLESWFGAGKEAKFRSKMRAGKEANNRARHRAKREACIDFMPGSIDVIKKESCFWPEENANTFSRPMIKKEARARAMTKEEAKTKARARAKQEARSEEEALIGTWFWATDESSMADEASIESSLQVEDESIIGSWFWTEEEASMGTGASSKSRPRTDGERIGDSLFGAREKTSMKTGAEATSESILAADDEQVIIGSWFWAGEEVNQEAEEETIFGSWFWVIDAASVESGVGVSCESRTRSEEEEVIGPWFWSGEQVDIEAGIGEEARPGAEEETIFGSWFWAENQTYMDCRAETSCDTMQGAEEEEPIIGSWFWTRVEACVEGDVNSKSSLEDKEEAMIPCFGAKEEVSMKHGTGVRCRFMAGAEETNNKSCFWAEKEPCMYPAGGGSWKSRPEEEEDIVNSWFWSRKYTKPEAIIGSWLWATEESNIDGTGEKAKLLTEEETIINSWFWKEDEAISEATDREESRPEAEEGDIIGSWFWAGEEDRLEPAAETREEDRLAAEKEGIVGSWFGAREETIRREAGSCSKSSPKAEEEEVIIGSWFWEEEASPEAVAGVGFESKPGTEEEEITVGSWFWPEEEASIQAGSQAVEEMESETEEETIFGSWFWDGKEVSEEAGPCCVSKPEDDEEMIVESWFWSRDKAIKETGTVATCESKPENEEGAIVGSWFEAEDEVDNRTDNGSNCGSRTLADEDEAIVGSWFWAGDEAHFESNPSPVFRAICRSTCSVEQEPDPSRRPQSWEEVTVQFKPGPWGRVGFPSISPFRFPKEAASLFCEMFGGKPRNMVLSPEGEDQESLLQPDQPSPEFPFQYDPSYRSVQEIREHLRAKESTEPESSSCNCIQCELKIGSEEFEELLLLMEKIRDPFIHEISKIAMGMRSASQFTRDFIRDSGVVSLIETLLNYPSSRVRTSFLENMIRMAPPYPNLNIIQTYICKVCEETLAYSVDSPEQLSGIRMIRHLTTTTDYHTLVANYMSGFLSLLATGNAKTRFHVLKMLLNLSENLFMTKELLSAEAVSEFIGLFNREETNDNIQIVLAIFENIGNNIKKETVFSDDDFNIEPLISAFHKVEKFAKELQGKTDNQNDPEGDQEN

Target ORF information:

RefSeq Version	NM_014710.4
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014710.4

ORF Insert Sequence:

ATGACTGGGG CAGAGATTGA GTCTGGTGCC CAGGTCAAGC CTGAAAAGAA GCCTGGGGAA 
GAGGTTGTAG GTGGGGCTGA GATAGAGAAT GATGTCCCTC TGGTGGTCAG ACCCAAGGTT 
AGGACCCAGG CCCAGATAAT GCCTGGGGCA AGGCCCAAGA ATAAGTCCAA GGTTATGCCT 
GGAGCAAGCA CCAAAGTTGA GACAAGTGCA GTGGGTGGGG CACGCCCTAA GAGTAAGGCC 
AAGGCAATAC CTGTTTCACG ATTTAAGGAA GAAGCCCAGA TGTGGGCTCA GCCCAGGTTT 
GGTGCTGAAA GATTGTCTAA GACAGAGAGA AACTCCCAGA CCAATATCAT AGCCTCTCCA 
CTTGTCAGTA CTGATTCTGT CTTGGTTGCT AAAACAAAGT ACCTGTCTGA GGATAGAGAA 
CTGGTTAATA CAGACACTGA GAGCTTTCCT AGAAGGAAGG CCCATTACCA AGCAGGATTC 
CAGCCTTCTT TTAGGTCAAA GGAGGAGACC AATATGGGGT CCTGGTGCTG TCCTAGGCCT 
ACATCCAAAC AAGAAGCCTC TCCTAATTCT GATTTCAAAT GGGTAGACAA ATCTGTGAGT 
TCCTTGTTCT GGAGTGGAGA TGAGGTCACT GCAAAATTTC ATCCTGGGAA TAGGGTAAAA 
GACAGTAACA GATCCATGCA CATGGCCAAT CAAGAGGCTA ATACCATGTC TAGGTCCCAA 
ACTAACCAGG AGCTCTATAT TGCATCTAGT TCTGGTTCTG AGGATGAGTC TGTTAAGACA 
CCCTGGTTCT GGGCCAGAGA TAAAACCAAT ACCTGGTCTG GGCCCAGGGA AGATCCCAAT 
AGCAGGTCCA GGTTTAGGTC TAAGAAAGAA GTCTATGTTG AATCAAGTTC TGGATCTGAG 
CATGAAGACC ATTTGGAGTC CTGGTTTGGG GCTGGAAAGG AGGCCAAATT CAGGTCCAAA 
ATGAGAGCTG GGAAGGAGGC CAATAACAGG GCCAGGCACA GGGCCAAGCG AGAAGCTTGC 
ATTGATTTCA TGCCTGGGTC TATAGATGTA ATTAAAAAAG AGTCCTGTTT CTGGCCTGAA 
GAAAATGCTA ATACCTTTTC AAGGCCCATG ATCAAGAAAG AGGCCAGGGC CAGAGCAATG 
ACAAAGGAAG AGGCCAAAAC CAAGGCCCGA GCCAGGGCCA AGCAAGAAGC CAGGTCAGAG 
GAGGAAGCCC TCATTGGGAC CTGGTTCTGG GCTACAGACG AGTCCAGCAT GGCAGATGAA 
GCCAGCATAG AGTCCAGTCT ACAAGTGGAG GATGAGTCCA TAATTGGGAG TTGGTTCTGG 
ACTGAAGAAG AGGCCAGTAT GGGGACTGGG GCTAGCAGTA AATCCAGACC AAGGACTGAT 
GGGGAGCGTA TTGGTGATTC CTTATTTGGG GCTAGGGAAA AGACCAGTAT GAAAACTGGG 
GCTGAGGCCA CCTCTGAATC TATACTAGCA GCTGATGATG AACAGGTCAT TATTGGTTCC 
TGGTTCTGGG CTGGTGAAGA GGTCAACCAA GAGGCTGAGG AAGAGACCAT TTTTGGGTCG 
TGGTTCTGGG TCATTGATGC GGCCAGTGTG GAATCTGGTG TTGGGGTCAG CTGTGAGTCC 
AGGACAAGGT CTGAGGAAGA AGAGGTCATT GGTCCCTGGT TTTGGTCTGG AGAACAAGTT 
GATATAGAGG CTGGAATCGG AGAAGAGGCC AGGCCAGGAG CTGAAGAAGA GACAATATTC 
GGGTCCTGGT TTTGGGCTGA AAACCAGACC TATATGGATT GTAGGGCTGA AACTAGCTGT 
GACACCATGC AAGGGGCTGA GGAGGAGGAG CCCATTATTG GGTCCTGGTT TTGGACCAGA 
GTAGAAGCTT GTGTGGAGGG TGATGTCAAC AGCAAGTCTA GCCTGGAGGA CAAGGAAGAG 
GCCATGATAC CATGTTTTGG AGCCAAAGAA GAGGTCAGTA TGAAGCATGG GACTGGTGTC 
AGATGCAGAT TTATGGCAGG GGCTGAGGAG ACCAATAATA AGTCTTGCTT CTGGGCAGAA 
AAAGAACCCT GTATGTATCC TGCCGGTGGA GGAAGTTGGA AGTCTAGGCC AGAGGAGGAA 
GAGGACATTG TCAATTCGTG GTTCTGGTCC AGAAAATACA CAAAGCCAGA GGCCATTATA 
GGGTCCTGGT TATGGGCTAC AGAAGAGAGT AATATAGATG GGACTGGAGA AAAGGCCAAG 
TTACTGACTG AAGAGGAGAC CATAATCAAT TCCTGGTTCT GGAAAGAAGA TGAAGCCATT 
TCAGAGGCTA CTGACAGAGA AGAGTCCAGG CCAGAAGCTG AGGAGGGGGA CATTATTGGT 
TCTTGGTTCT GGGCTGGAGA AGAGGACAGA CTAGAGCCAG CTGCTGAGAC TAGAGAAGAA 
GACAGGCTAG CAGCTGAGAA AGAAGGTATT GTTGGGTCCT GGTTTGGGGC CAGAGAAGAG 
ACCATTAGAA GAGAGGCTGG GTCTTGCAGC AAATCCAGTC CTAAAGCTGA AGAGGAAGAA 
GTCATTATTG GGTCCTGGTT CTGGGAAGAA GAGGCCAGTC CGGAGGCAGT GGCAGGAGTC 
GGCTTTGAGT CAAAGCCTGG GACTGAGGAG GAAGAAATCA CTGTTGGGTC CTGGTTCTGG 
CCTGAAGAAG AAGCCAGTAT ACAGGCTGGA TCTCAGGCAG TAGAGGAAAT GGAGTCAGAG 
ACTGAAGAGG AAACCATTTT TGGGTCCTGG TTCTGGGATG GAAAAGAAGT CAGTGAAGAA 
GCAGGACCAT GCTGTGTATC CAAGCCAGAG GATGATGAAG AGATGATTGT TGAGTCCTGG 
TTCTGGTCTA GAGACAAAGC CATTAAGGAA ACTGGAACTG TGGCCACCTG TGAGTCCAAG 
CCAGAAAATG AGGAAGGGGC CATTGTTGGG TCTTGGTTTG AGGCTGAAGA TGAGGTAGAT 
AACAGGACTG ACAATGGAAG CAACTGTGGG TCCAGGACAT TAGCTGATGA AGATGAGGCC 
ATAGTGGGGT CCTGGTTCTG GGCAGGAGAT GAGGCCCATT TTGAATCAAA TCCTAGCCCC 
GTGTTCAGGG CCATTTGCAG GTCCACGTGT TCAGTTGAAC AGGAGCCTGA TCCTTCACGC 
AGGCCTCAGA GTTGGGAGGA GGTCACTGTT CAGTTCAAGC CTGGTCCATG GGGTAGGGTC 
GGCTTCCCAT CTATAAGCCC CTTTAGATTT CCGAAAGAGG CAGCATCTTT ATTCTGTGAA 
ATGTTTGGGG GCAAACCCAG GAACATGGTA CTTAGCCCAG AAGGGGAAGA TCAGGAATCT 
TTGCTTCAGC CTGATCAGCC TAGTCCTGAG TTCCCATTTC AGTATGATCC TTCCTACAGG 
TCAGTCCAGG AAATTCGAGA GCATCTTAGG GCCAAGGAGA GTACAGAGCC TGAGAGTTCA 
TCCTGTAACT GCATACAATG TGAGCTGAAA ATTGGTTCTG AAGAGTTTGA AGAACTCCTT 
TTATTAATGG AAAAAATTCG GGATCCTTTT ATTCATGAAA TATCTAAAAT CGCAATGGGT 
ATGAGAAGTG CTTCTCAATT TACCCGAGAT TTCATTCGAG ATTCAGGTGT TGTCTCACTT 
ATTGAAACCT TGCTTAATTA TCCGTCCTCC CGAGTTAGAA CAAGTTTTTT GGAAAATATG 
ATTCGCATGG CCCCACCTTA TCCGAATCTA AACATAATTC AGACATACAT ATGTAAAGTG 
TGTGAGGAAA CCCTTGCTTA TAGCGTGGAT TCCCCGGAAC AGCTGTCTGG AATAAGGATG 
ATTAGACATC TCACTACTAC TACTGACTAT CACACACTGG TTGCCAATTA TATGTCTGGG 
TTTCTCTCCT TATTAGCTAC AGGCAATGCC AAAACAAGGT TTCATGTTTT GAAAATGCTA 
CTGAATTTGT CTGAAAATCT TTTCATGACA AAAGAACTAC TCAGTGCTGA AGCAGTGTCA 
GAATTTATAG GCCTCTTTAA CAGGGAAGAG ACAAATGACA ATATTCAAAT TGTTCTTGCA 
ATATTTGAGA ATATTGGCAA CAATATCAAA AAAGAAACAG TGTTCTCTGA TGATGATTTC 
AATATTGAGC CGCTTATTTC TGCATTCCAC AAAGTTGAGA AATTTGCTAA GGAACTGCAA 
GGCAAAACAG ACAATCAAAA TGACCCTGAA GGGGACCAAG AAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPRASP1 ( NM_001184727.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001171656.1 Homo sapiens G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu13433
Clone ID Related Accession (Same CDS sequence)	NM_014710.5 , NM_001099411.1 , NM_001184727.1 , XM_017029981.1 , XM_017029982.1 , NM_014710.4 , NM_001184727.2 , NM_001099410.1
Accession Version	NM_001184727.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	G-protein coupled receptor-associated sorting protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX954816.1, BX489726.1, BC114552.1, AY044233.1 and AW293629.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_001184727.1. Transcript Variant: This variant (4) has an additional exon in the 5' UTR and encodes the same protein, as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.256013.1, SRR1803613.156277.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000537097.2/ ENSP00000445683.1 RefSeq Select criteria :: based on expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACTGGGG CAGAGATTGA GTCTGGTGCC CAGGTCAAGC CTGAAAAGAA GCCTGGGGAA 
GAGGTTGTAG GTGGGGCTGA GATAGAGAAT GATGTCCCTC TGGTGGTCAG ACCCAAGGTT 
AGGACCCAGG CCCAGATAAT GCCTGGGGCA AGGCCCAAGA ATAAGTCCAA GGTTATGCCT 
GGAGCAAGCA CCAAAGTTGA GACAAGTGCA GTGGGTGGGG CACGCCCTAA GAGTAAGGCC 
AAGGCAATAC CTGTTTCACG ATTTAAGGAA GAAGCCCAGA TGTGGGCTCA GCCCAGGTTT 
GGTGCTGAAA GATTGTCTAA GACAGAGAGA AACTCCCAGA CCAATATCAT AGCCTCTCCA 
CTTGTCAGTA CTGATTCTGT CTTGGTTGCT AAAACAAAGT ACCTGTCTGA GGATAGAGAA 
CTGGTTAATA CAGACACTGA GAGCTTTCCT AGAAGGAAGG CCCATTACCA AGCAGGATTC 
CAGCCTTCTT TTAGGTCAAA GGAGGAGACC AATATGGGGT CCTGGTGCTG TCCTAGGCCT 
ACATCCAAAC AAGAAGCCTC TCCTAATTCT GATTTCAAAT GGGTAGACAA ATCTGTGAGT 
TCCTTGTTCT GGAGTGGAGA TGAGGTCACT GCAAAATTTC ATCCTGGGAA TAGGGTAAAA 
GACAGTAACA GATCCATGCA CATGGCCAAT CAAGAGGCTA ATACCATGTC TAGGTCCCAA 
ACTAACCAGG AGCTCTATAT TGCATCTAGT TCTGGTTCTG AGGATGAGTC TGTTAAGACA 
CCCTGGTTCT GGGCCAGAGA TAAAACCAAT ACCTGGTCTG GGCCCAGGGA AGATCCCAAT 
AGCAGGTCCA GGTTTAGGTC TAAGAAAGAA GTCTATGTTG AATCAAGTTC TGGATCTGAG 
CATGAAGACC ATTTGGAGTC CTGGTTTGGG GCTGGAAAGG AGGCCAAATT CAGGTCCAAA 
ATGAGAGCTG GGAAGGAGGC CAATAACAGG GCCAGGCACA GGGCCAAGCG AGAAGCTTGC 
ATTGATTTCA TGCCTGGGTC TATAGATGTA ATTAAAAAAG AGTCCTGTTT CTGGCCTGAA 
GAAAATGCTA ATACCTTTTC AAGGCCCATG ATCAAGAAAG AGGCCAGGGC CAGAGCAATG 
ACAAAGGAAG AGGCCAAAAC CAAGGCCCGA GCCAGGGCCA AGCAAGAAGC CAGGTCAGAG 
GAGGAAGCCC TCATTGGGAC CTGGTTCTGG GCTACAGACG AGTCCAGCAT GGCAGATGAA 
GCCAGCATAG AGTCCAGTCT ACAAGTGGAG GATGAGTCCA TAATTGGGAG TTGGTTCTGG 
ACTGAAGAAG AGGCCAGTAT GGGGACTGGG GCTAGCAGTA AATCCAGACC AAGGACTGAT 
GGGGAGCGTA TTGGTGATTC CTTATTTGGG GCTAGGGAAA AGACCAGTAT GAAAACTGGG 
GCTGAGGCCA CCTCTGAATC TATACTAGCA GCTGATGATG AACAGGTCAT TATTGGTTCC 
TGGTTCTGGG CTGGTGAAGA GGTCAACCAA GAGGCTGAGG AAGAGACCAT TTTTGGGTCG 
TGGTTCTGGG TCATTGATGC GGCCAGTGTG GAATCTGGTG TTGGGGTCAG CTGTGAGTCC 
AGGACAAGGT CTGAGGAAGA AGAGGTCATT GGTCCCTGGT TTTGGTCTGG AGAACAAGTT 
GATATAGAGG CTGGAATCGG AGAAGAGGCC AGGCCAGGAG CTGAAGAAGA GACAATATTC 
GGGTCCTGGT TTTGGGCTGA AAACCAGACC TATATGGATT GTAGGGCTGA AACTAGCTGT 
GACACCATGC AAGGGGCTGA GGAGGAGGAG CCCATTATTG GGTCCTGGTT TTGGACCAGA 
GTAGAAGCTT GTGTGGAGGG TGATGTCAAC AGCAAGTCTA GCCTGGAGGA CAAGGAAGAG 
GCCATGATAC CATGTTTTGG AGCCAAAGAA GAGGTCAGTA TGAAGCATGG GACTGGTGTC 
AGATGCAGAT TTATGGCAGG GGCTGAGGAG ACCAATAATA AGTCTTGCTT CTGGGCAGAA 
AAAGAACCCT GTATGTATCC TGCCGGTGGA GGAAGTTGGA AGTCTAGGCC AGAGGAGGAA 
GAGGACATTG TCAATTCGTG GTTCTGGTCC AGAAAATACA CAAAGCCAGA GGCCATTATA 
GGGTCCTGGT TATGGGCTAC AGAAGAGAGT AATATAGATG GGACTGGAGA AAAGGCCAAG 
TTACTGACTG AAGAGGAGAC CATAATCAAT TCCTGGTTCT GGAAAGAAGA TGAAGCCATT 
TCAGAGGCTA CTGACAGAGA AGAGTCCAGG CCAGAAGCTG AGGAGGGGGA CATTATTGGT 
TCTTGGTTCT GGGCTGGAGA AGAGGACAGA CTAGAGCCAG CTGCTGAGAC TAGAGAAGAA 
GACAGGCTAG CAGCTGAGAA AGAAGGTATT GTTGGGTCCT GGTTTGGGGC CAGAGAAGAG 
ACCATTAGAA GAGAGGCTGG GTCTTGCAGC AAATCCAGTC CTAAAGCTGA AGAGGAAGAA 
GTCATTATTG GGTCCTGGTT CTGGGAAGAA GAGGCCAGTC CGGAGGCAGT GGCAGGAGTC 
GGCTTTGAGT CAAAGCCTGG GACTGAGGAG GAAGAAATCA CTGTTGGGTC CTGGTTCTGG 
CCTGAAGAAG AAGCCAGTAT ACAGGCTGGA TCTCAGGCAG TAGAGGAAAT GGAGTCAGAG 
ACTGAAGAGG AAACCATTTT TGGGTCCTGG TTCTGGGATG GAAAAGAAGT CAGTGAAGAA 
GCAGGACCAT GCTGTGTATC CAAGCCAGAG GATGATGAAG AGATGATTGT TGAGTCCTGG 
TTCTGGTCTA GAGACAAAGC CATTAAGGAA ACTGGAACTG TGGCCACCTG TGAGTCCAAG 
CCAGAAAATG AGGAAGGGGC CATTGTTGGG TCTTGGTTTG AGGCTGAAGA TGAGGTAGAT 
AACAGGACTG ACAATGGAAG CAACTGTGGG TCCAGGACAT TAGCTGATGA AGATGAGGCC 
ATAGTGGGGT CCTGGTTCTG GGCAGGAGAT GAGGCCCATT TTGAATCAAA TCCTAGCCCC 
GTGTTCAGGG CCATTTGCAG GTCCACGTGT TCAGTTGAAC AGGAGCCTGA TCCTTCACGC 
AGGCCTCAGA GTTGGGAGGA GGTCACTGTT CAGTTCAAGC CTGGTCCATG GGGTAGGGTC 
GGCTTCCCAT CTATAAGCCC CTTTAGATTT CCGAAAGAGG CAGCATCTTT ATTCTGTGAA 
ATGTTTGGGG GCAAACCCAG GAACATGGTA CTTAGCCCAG AAGGGGAAGA TCAGGAATCT 
TTGCTTCAGC CTGATCAGCC TAGTCCTGAG TTCCCATTTC AGTATGATCC TTCCTACAGG 
TCAGTCCAGG AAATTCGAGA GCATCTTAGG GCCAAGGAGA GTACAGAGCC TGAGAGTTCA 
TCCTGTAACT GCATACAATG TGAGCTGAAA ATTGGTTCTG AAGAGTTTGA AGAACTCCTT 
TTATTAATGG AAAAAATTCG GGATCCTTTT ATTCATGAAA TATCTAAAAT CGCAATGGGT 
ATGAGAAGTG CTTCTCAATT TACCCGAGAT TTCATTCGAG ATTCAGGTGT TGTCTCACTT 
ATTGAAACCT TGCTTAATTA TCCGTCCTCC CGAGTTAGAA CAAGTTTTTT GGAAAATATG 
ATTCGCATGG CCCCACCTTA TCCGAATCTA AACATAATTC AGACATACAT ATGTAAAGTG 
TGTGAGGAAA CCCTTGCTTA TAGCGTGGAT TCCCCGGAAC AGCTGTCTGG AATAAGGATG 
ATTAGACATC TCACTACTAC TACTGACTAT CACACACTGG TTGCCAATTA TATGTCTGGG 
TTTCTCTCCT TATTAGCTAC AGGCAATGCC AAAACAAGGT TTCATGTTTT GAAAATGCTA 
CTGAATTTGT CTGAAAATCT TTTCATGACA AAAGAACTAC TCAGTGCTGA AGCAGTGTCA 
GAATTTATAG GCCTCTTTAA CAGGGAAGAG ACAAATGACA ATATTCAAAT TGTTCTTGCA 
ATATTTGAGA ATATTGGCAA CAATATCAAA AAAGAAACAG TGTTCTCTGA TGATGATTTC 
AATATTGAGC CGCTTATTTC TGCATTCCAC AAAGTTGAGA AATTTGCTAA GGAACTGCAA 
GGCAAAACAG ACAATCAAAA TGACCCTGAA GGGGACCAAG AAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001171656.1
CDS	732..4919
Translation	MTGAEIESGAQVKPEKKPGEEVVGGAEIENDVPLVVRPKVRTQAQIMPGARPKNKSKVMPGASTKVETSAVGGARPKSKAKAIPVSRFKEEAQMWAQPRFGAERLSKTERNSQTNIIASPLVSTDSVLVAKTKYLSEDRELVNTDTESFPRRKAHYQAGFQPSFRSKEETNMGSWCCPRPTSKQEASPNSDFKWVDKSVSSLFWSGDEVTAKFHPGNRVKDSNRSMHMANQEANTMSRSQTNQELYIASSSGSEDESVKTPWFWARDKTNTWSGPREDPNSRSRFRSKKEVYVESSSGSEHEDHLESWFGAGKEAKFRSKMRAGKEANNRARHRAKREACIDFMPGSIDVIKKESCFWPEENANTFSRPMIKKEARARAMTKEEAKTKARARAKQEARSEEEALIGTWFWATDESSMADEASIESSLQVEDESIIGSWFWTEEEASMGTGASSKSRPRTDGERIGDSLFGAREKTSMKTGAEATSESILAADDEQVIIGSWFWAGEEVNQEAEEETIFGSWFWVIDAASVESGVGVSCESRTRSEEEEVIGPWFWSGEQVDIEAGIGEEARPGAEEETIFGSWFWAENQTYMDCRAETSCDTMQGAEEEEPIIGSWFWTRVEACVEGDVNSKSSLEDKEEAMIPCFGAKEEVSMKHGTGVRCRFMAGAEETNNKSCFWAEKEPCMYPAGGGSWKSRPEEEEDIVNSWFWSRKYTKPEAIIGSWLWATEESNIDGTGEKAKLLTEEETIINSWFWKEDEAISEATDREESRPEAEEGDIIGSWFWAGEEDRLEPAAETREEDRLAAEKEGIVGSWFGAREETIRREAGSCSKSSPKAEEEEVIIGSWFWEEEASPEAVAGVGFESKPGTEEEEITVGSWFWPEEEASIQAGSQAVEEMESETEEETIFGSWFWDGKEVSEEAGPCCVSKPEDDEEMIVESWFWSRDKAIKETGTVATCESKPENEEGAIVGSWFEAEDEVDNRTDNGSNCGSRTLADEDEAIVGSWFWAGDEAHFESNPSPVFRAICRSTCSVEQEPDPSRRPQSWEEVTVQFKPGPWGRVGFPSISPFRFPKEAASLFCEMFGGKPRNMVLSPEGEDQESLLQPDQPSPEFPFQYDPSYRSVQEIREHLRAKESTEPESSSCNCIQCELKIGSEEFEELLLLMEKIRDPFIHEISKIAMGMRSASQFTRDFIRDSGVVSLIETLLNYPSSRVRTSFLENMIRMAPPYPNLNIIQTYICKVCEETLAYSVDSPEQLSGIRMIRHLTTTTDYHTLVANYMSGFLSLLATGNAKTRFHVLKMLLNLSENLFMTKELLSAEAVSEFIGLFNREETNDNIQIVLAIFENIGNNIKKETVFSDDDFNIEPLISAFHKVEKFAKELQGKTDNQNDPEGDQEN

Target ORF information:

RefSeq Version	NM_001184727.2
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001184727.2

ORF Insert Sequence:

ATGACTGGGG CAGAGATTGA GTCTGGTGCC CAGGTCAAGC CTGAAAAGAA GCCTGGGGAA 
GAGGTTGTAG GTGGGGCTGA GATAGAGAAT GATGTCCCTC TGGTGGTCAG ACCCAAGGTT 
AGGACCCAGG CCCAGATAAT GCCTGGGGCA AGGCCCAAGA ATAAGTCCAA GGTTATGCCT 
GGAGCAAGCA CCAAAGTTGA GACAAGTGCA GTGGGTGGGG CACGCCCTAA GAGTAAGGCC 
AAGGCAATAC CTGTTTCACG ATTTAAGGAA GAAGCCCAGA TGTGGGCTCA GCCCAGGTTT 
GGTGCTGAAA GATTGTCTAA GACAGAGAGA AACTCCCAGA CCAATATCAT AGCCTCTCCA 
CTTGTCAGTA CTGATTCTGT CTTGGTTGCT AAAACAAAGT ACCTGTCTGA GGATAGAGAA 
CTGGTTAATA CAGACACTGA GAGCTTTCCT AGAAGGAAGG CCCATTACCA AGCAGGATTC 
CAGCCTTCTT TTAGGTCAAA GGAGGAGACC AATATGGGGT CCTGGTGCTG TCCTAGGCCT 
ACATCCAAAC AAGAAGCCTC TCCTAATTCT GATTTCAAAT GGGTAGACAA ATCTGTGAGT 
TCCTTGTTCT GGAGTGGAGA TGAGGTCACT GCAAAATTTC ATCCTGGGAA TAGGGTAAAA 
GACAGTAACA GATCCATGCA CATGGCCAAT CAAGAGGCTA ATACCATGTC TAGGTCCCAA 
ACTAACCAGG AGCTCTATAT TGCATCTAGT TCTGGTTCTG AGGATGAGTC TGTTAAGACA 
CCCTGGTTCT GGGCCAGAGA TAAAACCAAT ACCTGGTCTG GGCCCAGGGA AGATCCCAAT 
AGCAGGTCCA GGTTTAGGTC TAAGAAAGAA GTCTATGTTG AATCAAGTTC TGGATCTGAG 
CATGAAGACC ATTTGGAGTC CTGGTTTGGG GCTGGAAAGG AGGCCAAATT CAGGTCCAAA 
ATGAGAGCTG GGAAGGAGGC CAATAACAGG GCCAGGCACA GGGCCAAGCG AGAAGCTTGC 
ATTGATTTCA TGCCTGGGTC TATAGATGTA ATTAAAAAAG AGTCCTGTTT CTGGCCTGAA 
GAAAATGCTA ATACCTTTTC AAGGCCCATG ATCAAGAAAG AGGCCAGGGC CAGAGCAATG 
ACAAAGGAAG AGGCCAAAAC CAAGGCCCGA GCCAGGGCCA AGCAAGAAGC CAGGTCAGAG 
GAGGAAGCCC TCATTGGGAC CTGGTTCTGG GCTACAGACG AGTCCAGCAT GGCAGATGAA 
GCCAGCATAG AGTCCAGTCT ACAAGTGGAG GATGAGTCCA TAATTGGGAG TTGGTTCTGG 
ACTGAAGAAG AGGCCAGTAT GGGGACTGGG GCTAGCAGTA AATCCAGACC AAGGACTGAT 
GGGGAGCGTA TTGGTGATTC CTTATTTGGG GCTAGGGAAA AGACCAGTAT GAAAACTGGG 
GCTGAGGCCA CCTCTGAATC TATACTAGCA GCTGATGATG AACAGGTCAT TATTGGTTCC 
TGGTTCTGGG CTGGTGAAGA GGTCAACCAA GAGGCTGAGG AAGAGACCAT TTTTGGGTCG 
TGGTTCTGGG TCATTGATGC GGCCAGTGTG GAATCTGGTG TTGGGGTCAG CTGTGAGTCC 
AGGACAAGGT CTGAGGAAGA AGAGGTCATT GGTCCCTGGT TTTGGTCTGG AGAACAAGTT 
GATATAGAGG CTGGAATCGG AGAAGAGGCC AGGCCAGGAG CTGAAGAAGA GACAATATTC 
GGGTCCTGGT TTTGGGCTGA AAACCAGACC TATATGGATT GTAGGGCTGA AACTAGCTGT 
GACACCATGC AAGGGGCTGA GGAGGAGGAG CCCATTATTG GGTCCTGGTT TTGGACCAGA 
GTAGAAGCTT GTGTGGAGGG TGATGTCAAC AGCAAGTCTA GCCTGGAGGA CAAGGAAGAG 
GCCATGATAC CATGTTTTGG AGCCAAAGAA GAGGTCAGTA TGAAGCATGG GACTGGTGTC 
AGATGCAGAT TTATGGCAGG GGCTGAGGAG ACCAATAATA AGTCTTGCTT CTGGGCAGAA 
AAAGAACCCT GTATGTATCC TGCCGGTGGA GGAAGTTGGA AGTCTAGGCC AGAGGAGGAA 
GAGGACATTG TCAATTCGTG GTTCTGGTCC AGAAAATACA CAAAGCCAGA GGCCATTATA 
GGGTCCTGGT TATGGGCTAC AGAAGAGAGT AATATAGATG GGACTGGAGA AAAGGCCAAG 
TTACTGACTG AAGAGGAGAC CATAATCAAT TCCTGGTTCT GGAAAGAAGA TGAAGCCATT 
TCAGAGGCTA CTGACAGAGA AGAGTCCAGG CCAGAAGCTG AGGAGGGGGA CATTATTGGT 
TCTTGGTTCT GGGCTGGAGA AGAGGACAGA CTAGAGCCAG CTGCTGAGAC TAGAGAAGAA 
GACAGGCTAG CAGCTGAGAA AGAAGGTATT GTTGGGTCCT GGTTTGGGGC CAGAGAAGAG 
ACCATTAGAA GAGAGGCTGG GTCTTGCAGC AAATCCAGTC CTAAAGCTGA AGAGGAAGAA 
GTCATTATTG GGTCCTGGTT CTGGGAAGAA GAGGCCAGTC CGGAGGCAGT GGCAGGAGTC 
GGCTTTGAGT CAAAGCCTGG GACTGAGGAG GAAGAAATCA CTGTTGGGTC CTGGTTCTGG 
CCTGAAGAAG AAGCCAGTAT ACAGGCTGGA TCTCAGGCAG TAGAGGAAAT GGAGTCAGAG 
ACTGAAGAGG AAACCATTTT TGGGTCCTGG TTCTGGGATG GAAAAGAAGT CAGTGAAGAA 
GCAGGACCAT GCTGTGTATC CAAGCCAGAG GATGATGAAG AGATGATTGT TGAGTCCTGG 
TTCTGGTCTA GAGACAAAGC CATTAAGGAA ACTGGAACTG TGGCCACCTG TGAGTCCAAG 
CCAGAAAATG AGGAAGGGGC CATTGTTGGG TCTTGGTTTG AGGCTGAAGA TGAGGTAGAT 
AACAGGACTG ACAATGGAAG CAACTGTGGG TCCAGGACAT TAGCTGATGA AGATGAGGCC 
ATAGTGGGGT CCTGGTTCTG GGCAGGAGAT GAGGCCCATT TTGAATCAAA TCCTAGCCCC 
GTGTTCAGGG CCATTTGCAG GTCCACGTGT TCAGTTGAAC AGGAGCCTGA TCCTTCACGC 
AGGCCTCAGA GTTGGGAGGA GGTCACTGTT CAGTTCAAGC CTGGTCCATG GGGTAGGGTC 
GGCTTCCCAT CTATAAGCCC CTTTAGATTT CCGAAAGAGG CAGCATCTTT ATTCTGTGAA 
ATGTTTGGGG GCAAACCCAG GAACATGGTA CTTAGCCCAG AAGGGGAAGA TCAGGAATCT 
TTGCTTCAGC CTGATCAGCC TAGTCCTGAG TTCCCATTTC AGTATGATCC TTCCTACAGG 
TCAGTCCAGG AAATTCGAGA GCATCTTAGG GCCAAGGAGA GTACAGAGCC TGAGAGTTCA 
TCCTGTAACT GCATACAATG TGAGCTGAAA ATTGGTTCTG AAGAGTTTGA AGAACTCCTT 
TTATTAATGG AAAAAATTCG GGATCCTTTT ATTCATGAAA TATCTAAAAT CGCAATGGGT 
ATGAGAAGTG CTTCTCAATT TACCCGAGAT TTCATTCGAG ATTCAGGTGT TGTCTCACTT 
ATTGAAACCT TGCTTAATTA TCCGTCCTCC CGAGTTAGAA CAAGTTTTTT GGAAAATATG 
ATTCGCATGG CCCCACCTTA TCCGAATCTA AACATAATTC AGACATACAT ATGTAAAGTG 
TGTGAGGAAA CCCTTGCTTA TAGCGTGGAT TCCCCGGAAC AGCTGTCTGG AATAAGGATG 
ATTAGACATC TCACTACTAC TACTGACTAT CACACACTGG TTGCCAATTA TATGTCTGGG 
TTTCTCTCCT TATTAGCTAC AGGCAATGCC AAAACAAGGT TTCATGTTTT GAAAATGCTA 
CTGAATTTGT CTGAAAATCT TTTCATGACA AAAGAACTAC TCAGTGCTGA AGCAGTGTCA 
GAATTTATAG GCCTCTTTAA CAGGGAAGAG ACAAATGACA ATATTCAAAT TGTTCTTGCA 
ATATTTGAGA ATATTGGCAA CAATATCAAA AAAGAAACAG TGTTCTCTGA TGATGATTTC 
AATATTGAGC CGCTTATTTC TGCATTCCAC AAAGTTGAGA AATTTGCTAA GGAACTGCAA 
GGCAAAACAG ACAATCAAAA TGACCCTGAA GGGGACCAAG AAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPRASP1 ( NM_001099410.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001092880.1 Homo sapiens G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu13433
Clone ID Related Accession (Same CDS sequence)	NM_014710.5 , NM_001099411.1 , NM_001184727.1 , XM_017029981.1 , XM_017029982.1 , NM_014710.4 , NM_001184727.2 , NM_001099410.1
Accession Version	NM_001099410.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-23
Organism	Homo sapiens(human)
Product	G-protein coupled receptor-associated sorting protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP193624.1, DA104338.1, BC114552.1, AY044233.1 and AW293629.1. Transcript Variant: This variant (2) lacks an exon in the 5' UTR and encodes the same protein, as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.198005.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTGGGG CAGAGATTGA GTCTGGTGCC CAGGTCAAGC CTGAAAAGAA GCCTGGGGAA 
GAGGTTGTAG GTGGGGCTGA GATAGAGAAT GATGTCCCTC TGGTGGTCAG ACCCAAGGTT 
AGGACCCAGG CCCAGATAAT GCCTGGGGCA AGGCCCAAGA ATAAGTCCAA GGTTATGCCT 
GGAGCAAGCA CCAAAGTTGA GACAAGTGCA GTGGGTGGGG CACGCCCTAA GAGTAAGGCC 
AAGGCAATAC CTGTTTCACG ATTTAAGGAA GAAGCCCAGA TGTGGGCTCA GCCCAGGTTT 
GGTGCTGAAA GATTGTCTAA GACAGAGAGA AACTCCCAGA CCAATATCAT AGCCTCTCCA 
CTTGTCAGTA CTGATTCTGT CTTGGTTGCT AAAACAAAGT ACCTGTCTGA GGATAGAGAA 
CTGGTTAATA CAGACACTGA GAGCTTTCCT AGAAGGAAGG CCCATTACCA AGCAGGATTC 
CAGCCTTCTT TTAGGTCAAA GGAGGAGACC AATATGGGGT CCTGGTGCTG TCCTAGGCCT 
ACATCCAAAC AAGAAGCCTC TCCTAATTCT GATTTCAAAT GGGTAGACAA ATCTGTGAGT 
TCCTTGTTCT GGAGTGGAGA TGAGGTCACT GCAAAATTTC ATCCTGGGAA TAGGGTAAAA 
GACAGTAACA GATCCATGCA CATGGCCAAT CAAGAGGCTA ATACCATGTC TAGGTCCCAA 
ACTAACCAGG AGCTCTATAT TGCATCTAGT TCTGGTTCTG AGGATGAGTC TGTTAAGACA 
CCCTGGTTCT GGGCCAGAGA TAAAACCAAT ACCTGGTCTG GGCCCAGGGA AGATCCCAAT 
AGCAGGTCCA GGTTTAGGTC TAAGAAAGAA GTCTATGTTG AATCAAGTTC TGGATCTGAG 
CATGAAGACC ATTTGGAGTC CTGGTTTGGG GCTGGAAAGG AGGCCAAATT CAGGTCCAAA 
ATGAGAGCTG GGAAGGAGGC CAATAACAGG GCCAGGCACA GGGCCAAGCG AGAAGCTTGC 
ATTGATTTCA TGCCTGGGTC TATAGATGTA ATTAAAAAAG AGTCCTGTTT CTGGCCTGAA 
GAAAATGCTA ATACCTTTTC AAGGCCCATG ATCAAGAAAG AGGCCAGGGC CAGAGCAATG 
ACAAAGGAAG AGGCCAAAAC CAAGGCCCGA GCCAGGGCCA AGCAAGAAGC CAGGTCAGAG 
GAGGAAGCCC TCATTGGGAC CTGGTTCTGG GCTACAGACG AGTCCAGCAT GGCAGATGAA 
GCCAGCATAG AGTCCAGTCT ACAAGTGGAG GATGAGTCCA TAATTGGGAG TTGGTTCTGG 
ACTGAAGAAG AGGCCAGTAT GGGGACTGGG GCTAGCAGTA AATCCAGACC AAGGACTGAT 
GGGGAGCGTA TTGGTGATTC CTTATTTGGG GCTAGGGAAA AGACCAGTAT GAAAACTGGG 
GCTGAGGCCA CCTCTGAATC TATACTAGCA GCTGATGATG AACAGGTCAT TATTGGTTCC 
TGGTTCTGGG CTGGTGAAGA GGTCAACCAA GAGGCTGAGG AAGAGACCAT TTTTGGGTCG 
TGGTTCTGGG TCATTGATGC GGCCAGTGTG GAATCTGGTG TTGGGGTCAG CTGTGAGTCC 
AGGACAAGGT CTGAGGAAGA AGAGGTCATT GGTCCCTGGT TTTGGTCTGG AGAACAAGTT 
GATATAGAGG CTGGAATCGG AGAAGAGGCC AGGCCAGGAG CTGAAGAAGA GACAATATTC 
GGGTCCTGGT TTTGGGCTGA AAACCAGACC TATATGGATT GTAGGGCTGA AACTAGCTGT 
GACACCATGC AAGGGGCTGA GGAGGAGGAG CCCATTATTG GGTCCTGGTT TTGGACCAGA 
GTAGAAGCTT GTGTGGAGGG TGATGTCAAC AGCAAGTCTA GCCTGGAGGA CAAGGAAGAG 
GCCATGATAC CATGTTTTGG AGCCAAAGAA GAGGTCAGTA TGAAGCATGG GACTGGTGTC 
AGATGCAGAT TTATGGCAGG GGCTGAGGAG ACCAATAATA AGTCTTGCTT CTGGGCAGAA 
AAAGAACCCT GTATGTATCC TGCCGGTGGA GGAAGTTGGA AGTCTAGGCC AGAGGAGGAA 
GAGGACATTG TCAATTCGTG GTTCTGGTCC AGAAAATACA CAAAGCCAGA GGCCATTATA 
GGGTCCTGGT TATGGGCTAC AGAAGAGAGT AATATAGATG GGACTGGAGA AAAGGCCAAG 
TTACTGACTG AAGAGGAGAC CATAATCAAT TCCTGGTTCT GGAAAGAAGA TGAAGCCATT 
TCAGAGGCTA CTGACAGAGA AGAGTCCAGG CCAGAAGCTG AGGAGGGGGA CATTATTGGT 
TCTTGGTTCT GGGCTGGAGA AGAGGACAGA CTAGAGCCAG CTGCTGAGAC TAGAGAAGAA 
GACAGGCTAG CAGCTGAGAA AGAAGGTATT GTTGGGTCCT GGTTTGGGGC CAGAGAAGAG 
ACCATTAGAA GAGAGGCTGG GTCTTGCAGC AAATCCAGTC CTAAAGCTGA AGAGGAAGAA 
GTCATTATTG GGTCCTGGTT CTGGGAAGAA GAGGCCAGTC CGGAGGCAGT GGCAGGAGTC 
GGCTTTGAGT CAAAGCCTGG GACTGAGGAG GAAGAAATCA CTGTTGGGTC CTGGTTCTGG 
CCTGAAGAAG AAGCCAGTAT ACAGGCTGGA TCTCAGGCAG TAGAGGAAAT GGAGTCAGAG 
ACTGAAGAGG AAACCATTTT TGGGTCCTGG TTCTGGGATG GAAAAGAAGT CAGTGAAGAA 
GCAGGACCAT GCTGTGTATC CAAGCCAGAG GATGATGAAG AGATGATTGT TGAGTCCTGG 
TTCTGGTCTA GAGACAAAGC CATTAAGGAA ACTGGAACTG TGGCCACCTG TGAGTCCAAG 
CCAGAAAATG AGGAAGGGGC CATTGTTGGG TCTTGGTTTG AGGCTGAAGA TGAGGTAGAT 
AACAGGACTG ACAATGGAAG CAACTGTGGG TCCAGGACAT TAGCTGATGA AGATGAGGCC 
ATAGTGGGGT CCTGGTTCTG GGCAGGAGAT GAGGCCCATT TTGAATCAAA TCCTAGCCCC 
GTGTTCAGGG CCATTTGCAG GTCCACGTGT TCAGTTGAAC AGGAGCCTGA TCCTTCACGC 
AGGCCTCAGA GTTGGGAGGA GGTCACTGTT CAGTTCAAGC CTGGTCCATG GGGTAGGGTC 
GGCTTCCCAT CTATAAGCCC CTTTAGATTT CCGAAAGAGG CAGCATCTTT ATTCTGTGAA 
ATGTTTGGGG GCAAACCCAG GAACATGGTA CTTAGCCCAG AAGGGGAAGA TCAGGAATCT 
TTGCTTCAGC CTGATCAGCC TAGTCCTGAG TTCCCATTTC AGTATGATCC TTCCTACAGG 
TCAGTCCAGG AAATTCGAGA GCATCTTAGG GCCAAGGAGA GTACAGAGCC TGAGAGTTCA 
TCCTGTAACT GCATACAATG TGAGCTGAAA ATTGGTTCTG AAGAGTTTGA AGAACTCCTT 
TTATTAATGG AAAAAATTCG GGATCCTTTT ATTCATGAAA TATCTAAAAT CGCAATGGGT 
ATGAGAAGTG CTTCTCAATT TACCCGAGAT TTCATTCGAG ATTCAGGTGT TGTCTCACTT 
ATTGAAACCT TGCTTAATTA TCCGTCCTCC CGAGTTAGAA CAAGTTTTTT GGAAAATATG 
ATTCGCATGG CCCCACCTTA TCCGAATCTA AACATAATTC AGACATACAT ATGTAAAGTG 
TGTGAGGAAA CCCTTGCTTA TAGCGTGGAT TCCCCGGAAC AGCTGTCTGG AATAAGGATG 
ATTAGACATC TCACTACTAC TACTGACTAT CACACACTGG TTGCCAATTA TATGTCTGGG 
TTTCTCTCCT TATTAGCTAC AGGCAATGCC AAAACAAGGT TTCATGTTTT GAAAATGCTA 
CTGAATTTGT CTGAAAATCT TTTCATGACA AAAGAACTAC TCAGTGCTGA AGCAGTGTCA 
GAATTTATAG GCCTCTTTAA CAGGGAAGAG ACAAATGACA ATATTCAAAT TGTTCTTGCA 
ATATTTGAGA ATATTGGCAA CAATATCAAA AAAGAAACAG TGTTCTCTGA TGATGATTTC 
AATATTGAGC CGCTTATTTC TGCATTCCAC AAAGTTGAGA AATTTGCTAA GGAACTGCAA 
GGCAAAACAG ACAATCAAAA TGACCCTGAA GGGGACCAAG AAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001092880.1
CDS	711..4898
Translation	MTGAEIESGAQVKPEKKPGEEVVGGAEIENDVPLVVRPKVRTQAQIMPGARPKNKSKVMPGASTKVETSAVGGARPKSKAKAIPVSRFKEEAQMWAQPRFGAERLSKTERNSQTNIIASPLVSTDSVLVAKTKYLSEDRELVNTDTESFPRRKAHYQAGFQPSFRSKEETNMGSWCCPRPTSKQEASPNSDFKWVDKSVSSLFWSGDEVTAKFHPGNRVKDSNRSMHMANQEANTMSRSQTNQELYIASSSGSEDESVKTPWFWARDKTNTWSGPREDPNSRSRFRSKKEVYVESSSGSEHEDHLESWFGAGKEAKFRSKMRAGKEANNRARHRAKREACIDFMPGSIDVIKKESCFWPEENANTFSRPMIKKEARARAMTKEEAKTKARARAKQEARSEEEALIGTWFWATDESSMADEASIESSLQVEDESIIGSWFWTEEEASMGTGASSKSRPRTDGERIGDSLFGAREKTSMKTGAEATSESILAADDEQVIIGSWFWAGEEVNQEAEEETIFGSWFWVIDAASVESGVGVSCESRTRSEEEEVIGPWFWSGEQVDIEAGIGEEARPGAEEETIFGSWFWAENQTYMDCRAETSCDTMQGAEEEEPIIGSWFWTRVEACVEGDVNSKSSLEDKEEAMIPCFGAKEEVSMKHGTGVRCRFMAGAEETNNKSCFWAEKEPCMYPAGGGSWKSRPEEEEDIVNSWFWSRKYTKPEAIIGSWLWATEESNIDGTGEKAKLLTEEETIINSWFWKEDEAISEATDREESRPEAEEGDIIGSWFWAGEEDRLEPAAETREEDRLAAEKEGIVGSWFGAREETIRREAGSCSKSSPKAEEEEVIIGSWFWEEEASPEAVAGVGFESKPGTEEEEITVGSWFWPEEEASIQAGSQAVEEMESETEEETIFGSWFWDGKEVSEEAGPCCVSKPEDDEEMIVESWFWSRDKAIKETGTVATCESKPENEEGAIVGSWFEAEDEVDNRTDNGSNCGSRTLADEDEAIVGSWFWAGDEAHFESNPSPVFRAICRSTCSVEQEPDPSRRPQSWEEVTVQFKPGPWGRVGFPSISPFRFPKEAASLFCEMFGGKPRNMVLSPEGEDQESLLQPDQPSPEFPFQYDPSYRSVQEIREHLRAKESTEPESSSCNCIQCELKIGSEEFEELLLLMEKIRDPFIHEISKIAMGMRSASQFTRDFIRDSGVVSLIETLLNYPSSRVRTSFLENMIRMAPPYPNLNIIQTYICKVCEETLAYSVDSPEQLSGIRMIRHLTTTTDYHTLVANYMSGFLSLLATGNAKTRFHVLKMLLNLSENLFMTKELLSAEAVSEFIGLFNREETNDNIQIVLAIFENIGNNIKKETVFSDDDFNIEPLISAFHKVEKFAKELQGKTDNQNDPEGDQEN

Target ORF information:

RefSeq Version	NM_001099410.1
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001099410.1

ORF Insert Sequence:

ATGACTGGGG CAGAGATTGA GTCTGGTGCC CAGGTCAAGC CTGAAAAGAA GCCTGGGGAA 
GAGGTTGTAG GTGGGGCTGA GATAGAGAAT GATGTCCCTC TGGTGGTCAG ACCCAAGGTT 
AGGACCCAGG CCCAGATAAT GCCTGGGGCA AGGCCCAAGA ATAAGTCCAA GGTTATGCCT 
GGAGCAAGCA CCAAAGTTGA GACAAGTGCA GTGGGTGGGG CACGCCCTAA GAGTAAGGCC 
AAGGCAATAC CTGTTTCACG ATTTAAGGAA GAAGCCCAGA TGTGGGCTCA GCCCAGGTTT 
GGTGCTGAAA GATTGTCTAA GACAGAGAGA AACTCCCAGA CCAATATCAT AGCCTCTCCA 
CTTGTCAGTA CTGATTCTGT CTTGGTTGCT AAAACAAAGT ACCTGTCTGA GGATAGAGAA 
CTGGTTAATA CAGACACTGA GAGCTTTCCT AGAAGGAAGG CCCATTACCA AGCAGGATTC 
CAGCCTTCTT TTAGGTCAAA GGAGGAGACC AATATGGGGT CCTGGTGCTG TCCTAGGCCT 
ACATCCAAAC AAGAAGCCTC TCCTAATTCT GATTTCAAAT GGGTAGACAA ATCTGTGAGT 
TCCTTGTTCT GGAGTGGAGA TGAGGTCACT GCAAAATTTC ATCCTGGGAA TAGGGTAAAA 
GACAGTAACA GATCCATGCA CATGGCCAAT CAAGAGGCTA ATACCATGTC TAGGTCCCAA 
ACTAACCAGG AGCTCTATAT TGCATCTAGT TCTGGTTCTG AGGATGAGTC TGTTAAGACA 
CCCTGGTTCT GGGCCAGAGA TAAAACCAAT ACCTGGTCTG GGCCCAGGGA AGATCCCAAT 
AGCAGGTCCA GGTTTAGGTC TAAGAAAGAA GTCTATGTTG AATCAAGTTC TGGATCTGAG 
CATGAAGACC ATTTGGAGTC CTGGTTTGGG GCTGGAAAGG AGGCCAAATT CAGGTCCAAA 
ATGAGAGCTG GGAAGGAGGC CAATAACAGG GCCAGGCACA GGGCCAAGCG AGAAGCTTGC 
ATTGATTTCA TGCCTGGGTC TATAGATGTA ATTAAAAAAG AGTCCTGTTT CTGGCCTGAA 
GAAAATGCTA ATACCTTTTC AAGGCCCATG ATCAAGAAAG AGGCCAGGGC CAGAGCAATG 
ACAAAGGAAG AGGCCAAAAC CAAGGCCCGA GCCAGGGCCA AGCAAGAAGC CAGGTCAGAG 
GAGGAAGCCC TCATTGGGAC CTGGTTCTGG GCTACAGACG AGTCCAGCAT GGCAGATGAA 
GCCAGCATAG AGTCCAGTCT ACAAGTGGAG GATGAGTCCA TAATTGGGAG TTGGTTCTGG 
ACTGAAGAAG AGGCCAGTAT GGGGACTGGG GCTAGCAGTA AATCCAGACC AAGGACTGAT 
GGGGAGCGTA TTGGTGATTC CTTATTTGGG GCTAGGGAAA AGACCAGTAT GAAAACTGGG 
GCTGAGGCCA CCTCTGAATC TATACTAGCA GCTGATGATG AACAGGTCAT TATTGGTTCC 
TGGTTCTGGG CTGGTGAAGA GGTCAACCAA GAGGCTGAGG AAGAGACCAT TTTTGGGTCG 
TGGTTCTGGG TCATTGATGC GGCCAGTGTG GAATCTGGTG TTGGGGTCAG CTGTGAGTCC 
AGGACAAGGT CTGAGGAAGA AGAGGTCATT GGTCCCTGGT TTTGGTCTGG AGAACAAGTT 
GATATAGAGG CTGGAATCGG AGAAGAGGCC AGGCCAGGAG CTGAAGAAGA GACAATATTC 
GGGTCCTGGT TTTGGGCTGA AAACCAGACC TATATGGATT GTAGGGCTGA AACTAGCTGT 
GACACCATGC AAGGGGCTGA GGAGGAGGAG CCCATTATTG GGTCCTGGTT TTGGACCAGA 
GTAGAAGCTT GTGTGGAGGG TGATGTCAAC AGCAAGTCTA GCCTGGAGGA CAAGGAAGAG 
GCCATGATAC CATGTTTTGG AGCCAAAGAA GAGGTCAGTA TGAAGCATGG GACTGGTGTC 
AGATGCAGAT TTATGGCAGG GGCTGAGGAG ACCAATAATA AGTCTTGCTT CTGGGCAGAA 
AAAGAACCCT GTATGTATCC TGCCGGTGGA GGAAGTTGGA AGTCTAGGCC AGAGGAGGAA 
GAGGACATTG TCAATTCGTG GTTCTGGTCC AGAAAATACA CAAAGCCAGA GGCCATTATA 
GGGTCCTGGT TATGGGCTAC AGAAGAGAGT AATATAGATG GGACTGGAGA AAAGGCCAAG 
TTACTGACTG AAGAGGAGAC CATAATCAAT TCCTGGTTCT GGAAAGAAGA TGAAGCCATT 
TCAGAGGCTA CTGACAGAGA AGAGTCCAGG CCAGAAGCTG AGGAGGGGGA CATTATTGGT 
TCTTGGTTCT GGGCTGGAGA AGAGGACAGA CTAGAGCCAG CTGCTGAGAC TAGAGAAGAA 
GACAGGCTAG CAGCTGAGAA AGAAGGTATT GTTGGGTCCT GGTTTGGGGC CAGAGAAGAG 
ACCATTAGAA GAGAGGCTGG GTCTTGCAGC AAATCCAGTC CTAAAGCTGA AGAGGAAGAA 
GTCATTATTG GGTCCTGGTT CTGGGAAGAA GAGGCCAGTC CGGAGGCAGT GGCAGGAGTC 
GGCTTTGAGT CAAAGCCTGG GACTGAGGAG GAAGAAATCA CTGTTGGGTC CTGGTTCTGG 
CCTGAAGAAG AAGCCAGTAT ACAGGCTGGA TCTCAGGCAG TAGAGGAAAT GGAGTCAGAG 
ACTGAAGAGG AAACCATTTT TGGGTCCTGG TTCTGGGATG GAAAAGAAGT CAGTGAAGAA 
GCAGGACCAT GCTGTGTATC CAAGCCAGAG GATGATGAAG AGATGATTGT TGAGTCCTGG 
TTCTGGTCTA GAGACAAAGC CATTAAGGAA ACTGGAACTG TGGCCACCTG TGAGTCCAAG 
CCAGAAAATG AGGAAGGGGC CATTGTTGGG TCTTGGTTTG AGGCTGAAGA TGAGGTAGAT 
AACAGGACTG ACAATGGAAG CAACTGTGGG TCCAGGACAT TAGCTGATGA AGATGAGGCC 
ATAGTGGGGT CCTGGTTCTG GGCAGGAGAT GAGGCCCATT TTGAATCAAA TCCTAGCCCC 
GTGTTCAGGG CCATTTGCAG GTCCACGTGT TCAGTTGAAC AGGAGCCTGA TCCTTCACGC 
AGGCCTCAGA GTTGGGAGGA GGTCACTGTT CAGTTCAAGC CTGGTCCATG GGGTAGGGTC 
GGCTTCCCAT CTATAAGCCC CTTTAGATTT CCGAAAGAGG CAGCATCTTT ATTCTGTGAA 
ATGTTTGGGG GCAAACCCAG GAACATGGTA CTTAGCCCAG AAGGGGAAGA TCAGGAATCT 
TTGCTTCAGC CTGATCAGCC TAGTCCTGAG TTCCCATTTC AGTATGATCC TTCCTACAGG 
TCAGTCCAGG AAATTCGAGA GCATCTTAGG GCCAAGGAGA GTACAGAGCC TGAGAGTTCA 
TCCTGTAACT GCATACAATG TGAGCTGAAA ATTGGTTCTG AAGAGTTTGA AGAACTCCTT 
TTATTAATGG AAAAAATTCG GGATCCTTTT ATTCATGAAA TATCTAAAAT CGCAATGGGT 
ATGAGAAGTG CTTCTCAATT TACCCGAGAT TTCATTCGAG ATTCAGGTGT TGTCTCACTT 
ATTGAAACCT TGCTTAATTA TCCGTCCTCC CGAGTTAGAA CAAGTTTTTT GGAAAATATG 
ATTCGCATGG CCCCACCTTA TCCGAATCTA AACATAATTC AGACATACAT ATGTAAAGTG 
TGTGAGGAAA CCCTTGCTTA TAGCGTGGAT TCCCCGGAAC AGCTGTCTGG AATAAGGATG 
ATTAGACATC TCACTACTAC TACTGACTAT CACACACTGG TTGCCAATTA TATGTCTGGG 
TTTCTCTCCT TATTAGCTAC AGGCAATGCC AAAACAAGGT TTCATGTTTT GAAAATGCTA 
CTGAATTTGT CTGAAAATCT TTTCATGACA AAAGAACTAC TCAGTGCTGA AGCAGTGTCA 
GAATTTATAG GCCTCTTTAA CAGGGAAGAG ACAAATGACA ATATTCAAAT TGTTCTTGCA 
ATATTTGAGA ATATTGGCAA CAATATCAAA AAAGAAACAG TGTTCTCTGA TGATGATTTC 
AATATTGAGC CGCTTATTTC TGCATTCCAC AAAGTTGAGA AATTTGCTAA GGAACTGCAA 
GGCAAAACAG ACAATCAAAA TGACCCTGAA GGGGACCAAG AAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPRASP1 ( XM_017029981.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885470.1 Homo sapiens G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant X1, mRNA.

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CloneID	OHu13433
Clone ID Related Accession (Same CDS sequence)	NM_014710.5 , NM_001099411.1 , NM_001184727.1 , XM_017029981.1 , XM_017029982.1 , NM_014710.4 , NM_001184727.2 , NM_001099410.1
Accession Version	XM_017029981.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	G-protein coupled receptor-associated sorting protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGGGG CAGAGATTGA GTCTGGTGCC CAGGTCAAGC CTGAAAAGAA GCCTGGGGAA 
GAGGTTGTAG GTGGGGCTGA GATAGAGAAT GATGTCCCTC TGGTGGTCAG ACCCAAGGTT 
AGGACCCAGG CCCAGATAAT GCCTGGGGCA AGGCCCAAGA ATAAGTCCAA GGTTATGCCT 
GGAGCAAGCA CCAAAGTTGA GACAAGTGCA GTGGGTGGGG CACGCCCTAA GAGTAAGGCC 
AAGGCAATAC CTGTTTCACG ATTTAAGGAA GAAGCCCAGA TGTGGGCTCA GCCCAGGTTT 
GGTGCTGAAA GATTGTCTAA GACAGAGAGA AACTCCCAGA CCAATATCAT AGCCTCTCCA 
CTTGTCAGTA CTGATTCTGT CTTGGTTGCT AAAACAAAGT ACCTGTCTGA GGATAGAGAA 
CTGGTTAATA CAGACACTGA GAGCTTTCCT AGAAGGAAGG CCCATTACCA AGCAGGATTC 
CAGCCTTCTT TTAGGTCAAA GGAGGAGACC AATATGGGGT CCTGGTGCTG TCCTAGGCCT 
ACATCCAAAC AAGAAGCCTC TCCTAATTCT GATTTCAAAT GGGTAGACAA ATCTGTGAGT 
TCCTTGTTCT GGAGTGGAGA TGAGGTCACT GCAAAATTTC ATCCTGGGAA TAGGGTAAAA 
GACAGTAACA GATCCATGCA CATGGCCAAT CAAGAGGCTA ATACCATGTC TAGGTCCCAA 
ACTAACCAGG AGCTCTATAT TGCATCTAGT TCTGGTTCTG AGGATGAGTC TGTTAAGACA 
CCCTGGTTCT GGGCCAGAGA TAAAACCAAT ACCTGGTCTG GGCCCAGGGA AGATCCCAAT 
AGCAGGTCCA GGTTTAGGTC TAAGAAAGAA GTCTATGTTG AATCAAGTTC TGGATCTGAG 
CATGAAGACC ATTTGGAGTC CTGGTTTGGG GCTGGAAAGG AGGCCAAATT CAGGTCCAAA 
ATGAGAGCTG GGAAGGAGGC CAATAACAGG GCCAGGCACA GGGCCAAGCG AGAAGCTTGC 
ATTGATTTCA TGCCTGGGTC TATAGATGTA ATTAAAAAAG AGTCCTGTTT CTGGCCTGAA 
GAAAATGCTA ATACCTTTTC AAGGCCCATG ATCAAGAAAG AGGCCAGGGC CAGAGCAATG 
ACAAAGGAAG AGGCCAAAAC CAAGGCCCGA GCCAGGGCCA AGCAAGAAGC CAGGTCAGAG 
GAGGAAGCCC TCATTGGGAC CTGGTTCTGG GCTACAGACG AGTCCAGCAT GGCAGATGAA 
GCCAGCATAG AGTCCAGTCT ACAAGTGGAG GATGAGTCCA TAATTGGGAG TTGGTTCTGG 
ACTGAAGAAG AGGCCAGTAT GGGGACTGGG GCTAGCAGTA AATCCAGACC AAGGACTGAT 
GGGGAGCGTA TTGGTGATTC CTTATTTGGG GCTAGGGAAA AGACCAGTAT GAAAACTGGG 
GCTGAGGCCA CCTCTGAATC TATACTAGCA GCTGATGATG AACAGGTCAT TATTGGTTCC 
TGGTTCTGGG CTGGTGAAGA GGTCAACCAA GAGGCTGAGG AAGAGACCAT TTTTGGGTCG 
TGGTTCTGGG TCATTGATGC GGCCAGTGTG GAATCTGGTG TTGGGGTCAG CTGTGAGTCC 
AGGACAAGGT CTGAGGAAGA AGAGGTCATT GGTCCCTGGT TTTGGTCTGG AGAACAAGTT 
GATATAGAGG CTGGAATCGG AGAAGAGGCC AGGCCAGGAG CTGAAGAAGA GACAATATTC 
GGGTCCTGGT TTTGGGCTGA AAACCAGACC TATATGGATT GTAGGGCTGA AACTAGCTGT 
GACACCATGC AAGGGGCTGA GGAGGAGGAG CCCATTATTG GGTCCTGGTT TTGGACCAGA 
GTAGAAGCTT GTGTGGAGGG TGATGTCAAC AGCAAGTCTA GCCTGGAGGA CAAGGAAGAG 
GCCATGATAC CATGTTTTGG AGCCAAAGAA GAGGTCAGTA TGAAGCATGG GACTGGTGTC 
AGATGCAGAT TTATGGCAGG GGCTGAGGAG ACCAATAATA AGTCTTGCTT CTGGGCAGAA 
AAAGAACCCT GTATGTATCC TGCCGGTGGA GGAAGTTGGA AGTCTAGGCC AGAGGAGGAA 
GAGGACATTG TCAATTCGTG GTTCTGGTCC AGAAAATACA CAAAGCCAGA GGCCATTATA 
GGGTCCTGGT TATGGGCTAC AGAAGAGAGT AATATAGATG GGACTGGAGA AAAGGCCAAG 
TTACTGACTG AAGAGGAGAC CATAATCAAT TCCTGGTTCT GGAAAGAAGA TGAAGCCATT 
TCAGAGGCTA CTGACAGAGA AGAGTCCAGG CCAGAAGCTG AGGAGGGGGA CATTATTGGT 
TCTTGGTTCT GGGCTGGAGA AGAGGACAGA CTAGAGCCAG CTGCTGAGAC TAGAGAAGAA 
GACAGGCTAG CAGCTGAGAA AGAAGGTATT GTTGGGTCCT GGTTTGGGGC CAGAGAAGAG 
ACCATTAGAA GAGAGGCTGG GTCTTGCAGC AAATCCAGTC CTAAAGCTGA AGAGGAAGAA 
GTCATTATTG GGTCCTGGTT CTGGGAAGAA GAGGCCAGTC CGGAGGCAGT GGCAGGAGTC 
GGCTTTGAGT CAAAGCCTGG GACTGAGGAG GAAGAAATCA CTGTTGGGTC CTGGTTCTGG 
CCTGAAGAAG AAGCCAGTAT ACAGGCTGGA TCTCAGGCAG TAGAGGAAAT GGAGTCAGAG 
ACTGAAGAGG AAACCATTTT TGGGTCCTGG TTCTGGGATG GAAAAGAAGT CAGTGAAGAA 
GCAGGACCAT GCTGTGTATC CAAGCCAGAG GATGATGAAG AGATGATTGT TGAGTCCTGG 
TTCTGGTCTA GAGACAAAGC CATTAAGGAA ACTGGAACTG TGGCCACCTG TGAGTCCAAG 
CCAGAAAATG AGGAAGGGGC CATTGTTGGG TCTTGGTTTG AGGCTGAAGA TGAGGTAGAT 
AACAGGACTG ACAATGGAAG CAACTGTGGG TCCAGGACAT TAGCTGATGA AGATGAGGCC 
ATAGTGGGGT CCTGGTTCTG GGCAGGAGAT GAGGCCCATT TTGAATCAAA TCCTAGCCCC 
GTGTTCAGGG CCATTTGCAG GTCCACGTGT TCAGTTGAAC AGGAGCCTGA TCCTTCACGC 
AGGCCTCAGA GTTGGGAGGA GGTCACTGTT CAGTTCAAGC CTGGTCCATG GGGTAGGGTC 
GGCTTCCCAT CTATAAGCCC CTTTAGATTT CCGAAAGAGG CAGCATCTTT ATTCTGTGAA 
ATGTTTGGGG GCAAACCCAG GAACATGGTA CTTAGCCCAG AAGGGGAAGA TCAGGAATCT 
TTGCTTCAGC CTGATCAGCC TAGTCCTGAG TTCCCATTTC AGTATGATCC TTCCTACAGG 
TCAGTCCAGG AAATTCGAGA GCATCTTAGG GCCAAGGAGA GTACAGAGCC TGAGAGTTCA 
TCCTGTAACT GCATACAATG TGAGCTGAAA ATTGGTTCTG AAGAGTTTGA AGAACTCCTT 
TTATTAATGG AAAAAATTCG GGATCCTTTT ATTCATGAAA TATCTAAAAT CGCAATGGGT 
ATGAGAAGTG CTTCTCAATT TACCCGAGAT TTCATTCGAG ATTCAGGTGT TGTCTCACTT 
ATTGAAACCT TGCTTAATTA TCCGTCCTCC CGAGTTAGAA CAAGTTTTTT GGAAAATATG 
ATTCGCATGG CCCCACCTTA TCCGAATCTA AACATAATTC AGACATACAT ATGTAAAGTG 
TGTGAGGAAA CCCTTGCTTA TAGCGTGGAT TCCCCGGAAC AGCTGTCTGG AATAAGGATG 
ATTAGACATC TCACTACTAC TACTGACTAT CACACACTGG TTGCCAATTA TATGTCTGGG 
TTTCTCTCCT TATTAGCTAC AGGCAATGCC AAAACAAGGT TTCATGTTTT GAAAATGCTA 
CTGAATTTGT CTGAAAATCT TTTCATGACA AAAGAACTAC TCAGTGCTGA AGCAGTGTCA 
GAATTTATAG GCCTCTTTAA CAGGGAAGAG ACAAATGACA ATATTCAAAT TGTTCTTGCA 
ATATTTGAGA ATATTGGCAA CAATATCAAA AAAGAAACAG TGTTCTCTGA TGATGATTTC 
AATATTGAGC CGCTTATTTC TGCATTCCAC AAAGTTGAGA AATTTGCTAA GGAACTGCAA 
GGCAAAACAG ACAATCAAAA TGACCCTGAA GGGGACCAAG AAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885470.1
CDS	691..4878
Translation	MTGAEIESGAQVKPEKKPGEEVVGGAEIENDVPLVVRPKVRTQAQIMPGARPKNKSKVMPGASTKVETSAVGGARPKSKAKAIPVSRFKEEAQMWAQPRFGAERLSKTERNSQTNIIASPLVSTDSVLVAKTKYLSEDRELVNTDTESFPRRKAHYQAGFQPSFRSKEETNMGSWCCPRPTSKQEASPNSDFKWVDKSVSSLFWSGDEVTAKFHPGNRVKDSNRSMHMANQEANTMSRSQTNQELYIASSSGSEDESVKTPWFWARDKTNTWSGPREDPNSRSRFRSKKEVYVESSSGSEHEDHLESWFGAGKEAKFRSKMRAGKEANNRARHRAKREACIDFMPGSIDVIKKESCFWPEENANTFSRPMIKKEARARAMTKEEAKTKARARAKQEARSEEEALIGTWFWATDESSMADEASIESSLQVEDESIIGSWFWTEEEASMGTGASSKSRPRTDGERIGDSLFGAREKTSMKTGAEATSESILAADDEQVIIGSWFWAGEEVNQEAEEETIFGSWFWVIDAASVESGVGVSCESRTRSEEEEVIGPWFWSGEQVDIEAGIGEEARPGAEEETIFGSWFWAENQTYMDCRAETSCDTMQGAEEEEPIIGSWFWTRVEACVEGDVNSKSSLEDKEEAMIPCFGAKEEVSMKHGTGVRCRFMAGAEETNNKSCFWAEKEPCMYPAGGGSWKSRPEEEEDIVNSWFWSRKYTKPEAIIGSWLWATEESNIDGTGEKAKLLTEEETIINSWFWKEDEAISEATDREESRPEAEEGDIIGSWFWAGEEDRLEPAAETREEDRLAAEKEGIVGSWFGAREETIRREAGSCSKSSPKAEEEEVIIGSWFWEEEASPEAVAGVGFESKPGTEEEEITVGSWFWPEEEASIQAGSQAVEEMESETEEETIFGSWFWDGKEVSEEAGPCCVSKPEDDEEMIVESWFWSRDKAIKETGTVATCESKPENEEGAIVGSWFEAEDEVDNRTDNGSNCGSRTLADEDEAIVGSWFWAGDEAHFESNPSPVFRAICRSTCSVEQEPDPSRRPQSWEEVTVQFKPGPWGRVGFPSISPFRFPKEAASLFCEMFGGKPRNMVLSPEGEDQESLLQPDQPSPEFPFQYDPSYRSVQEIREHLRAKESTEPESSSCNCIQCELKIGSEEFEELLLLMEKIRDPFIHEISKIAMGMRSASQFTRDFIRDSGVVSLIETLLNYPSSRVRTSFLENMIRMAPPYPNLNIIQTYICKVCEETLAYSVDSPEQLSGIRMIRHLTTTTDYHTLVANYMSGFLSLLATGNAKTRFHVLKMLLNLSENLFMTKELLSAEAVSEFIGLFNREETNDNIQIVLAIFENIGNNIKKETVFSDDDFNIEPLISAFHKVEKFAKELQGKTDNQNDPEGDQEN

Target ORF information:

RefSeq Version	XM_017029981.1
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029981.1

ORF Insert Sequence:

ATGACTGGGG CAGAGATTGA GTCTGGTGCC CAGGTCAAGC CTGAAAAGAA GCCTGGGGAA 
GAGGTTGTAG GTGGGGCTGA GATAGAGAAT GATGTCCCTC TGGTGGTCAG ACCCAAGGTT 
AGGACCCAGG CCCAGATAAT GCCTGGGGCA AGGCCCAAGA ATAAGTCCAA GGTTATGCCT 
GGAGCAAGCA CCAAAGTTGA GACAAGTGCA GTGGGTGGGG CACGCCCTAA GAGTAAGGCC 
AAGGCAATAC CTGTTTCACG ATTTAAGGAA GAAGCCCAGA TGTGGGCTCA GCCCAGGTTT 
GGTGCTGAAA GATTGTCTAA GACAGAGAGA AACTCCCAGA CCAATATCAT AGCCTCTCCA 
CTTGTCAGTA CTGATTCTGT CTTGGTTGCT AAAACAAAGT ACCTGTCTGA GGATAGAGAA 
CTGGTTAATA CAGACACTGA GAGCTTTCCT AGAAGGAAGG CCCATTACCA AGCAGGATTC 
CAGCCTTCTT TTAGGTCAAA GGAGGAGACC AATATGGGGT CCTGGTGCTG TCCTAGGCCT 
ACATCCAAAC AAGAAGCCTC TCCTAATTCT GATTTCAAAT GGGTAGACAA ATCTGTGAGT 
TCCTTGTTCT GGAGTGGAGA TGAGGTCACT GCAAAATTTC ATCCTGGGAA TAGGGTAAAA 
GACAGTAACA GATCCATGCA CATGGCCAAT CAAGAGGCTA ATACCATGTC TAGGTCCCAA 
ACTAACCAGG AGCTCTATAT TGCATCTAGT TCTGGTTCTG AGGATGAGTC TGTTAAGACA 
CCCTGGTTCT GGGCCAGAGA TAAAACCAAT ACCTGGTCTG GGCCCAGGGA AGATCCCAAT 
AGCAGGTCCA GGTTTAGGTC TAAGAAAGAA GTCTATGTTG AATCAAGTTC TGGATCTGAG 
CATGAAGACC ATTTGGAGTC CTGGTTTGGG GCTGGAAAGG AGGCCAAATT CAGGTCCAAA 
ATGAGAGCTG GGAAGGAGGC CAATAACAGG GCCAGGCACA GGGCCAAGCG AGAAGCTTGC 
ATTGATTTCA TGCCTGGGTC TATAGATGTA ATTAAAAAAG AGTCCTGTTT CTGGCCTGAA 
GAAAATGCTA ATACCTTTTC AAGGCCCATG ATCAAGAAAG AGGCCAGGGC CAGAGCAATG 
ACAAAGGAAG AGGCCAAAAC CAAGGCCCGA GCCAGGGCCA AGCAAGAAGC CAGGTCAGAG 
GAGGAAGCCC TCATTGGGAC CTGGTTCTGG GCTACAGACG AGTCCAGCAT GGCAGATGAA 
GCCAGCATAG AGTCCAGTCT ACAAGTGGAG GATGAGTCCA TAATTGGGAG TTGGTTCTGG 
ACTGAAGAAG AGGCCAGTAT GGGGACTGGG GCTAGCAGTA AATCCAGACC AAGGACTGAT 
GGGGAGCGTA TTGGTGATTC CTTATTTGGG GCTAGGGAAA AGACCAGTAT GAAAACTGGG 
GCTGAGGCCA CCTCTGAATC TATACTAGCA GCTGATGATG AACAGGTCAT TATTGGTTCC 
TGGTTCTGGG CTGGTGAAGA GGTCAACCAA GAGGCTGAGG AAGAGACCAT TTTTGGGTCG 
TGGTTCTGGG TCATTGATGC GGCCAGTGTG GAATCTGGTG TTGGGGTCAG CTGTGAGTCC 
AGGACAAGGT CTGAGGAAGA AGAGGTCATT GGTCCCTGGT TTTGGTCTGG AGAACAAGTT 
GATATAGAGG CTGGAATCGG AGAAGAGGCC AGGCCAGGAG CTGAAGAAGA GACAATATTC 
GGGTCCTGGT TTTGGGCTGA AAACCAGACC TATATGGATT GTAGGGCTGA AACTAGCTGT 
GACACCATGC AAGGGGCTGA GGAGGAGGAG CCCATTATTG GGTCCTGGTT TTGGACCAGA 
GTAGAAGCTT GTGTGGAGGG TGATGTCAAC AGCAAGTCTA GCCTGGAGGA CAAGGAAGAG 
GCCATGATAC CATGTTTTGG AGCCAAAGAA GAGGTCAGTA TGAAGCATGG GACTGGTGTC 
AGATGCAGAT TTATGGCAGG GGCTGAGGAG ACCAATAATA AGTCTTGCTT CTGGGCAGAA 
AAAGAACCCT GTATGTATCC TGCCGGTGGA GGAAGTTGGA AGTCTAGGCC AGAGGAGGAA 
GAGGACATTG TCAATTCGTG GTTCTGGTCC AGAAAATACA CAAAGCCAGA GGCCATTATA 
GGGTCCTGGT TATGGGCTAC AGAAGAGAGT AATATAGATG GGACTGGAGA AAAGGCCAAG 
TTACTGACTG AAGAGGAGAC CATAATCAAT TCCTGGTTCT GGAAAGAAGA TGAAGCCATT 
TCAGAGGCTA CTGACAGAGA AGAGTCCAGG CCAGAAGCTG AGGAGGGGGA CATTATTGGT 
TCTTGGTTCT GGGCTGGAGA AGAGGACAGA CTAGAGCCAG CTGCTGAGAC TAGAGAAGAA 
GACAGGCTAG CAGCTGAGAA AGAAGGTATT GTTGGGTCCT GGTTTGGGGC CAGAGAAGAG 
ACCATTAGAA GAGAGGCTGG GTCTTGCAGC AAATCCAGTC CTAAAGCTGA AGAGGAAGAA 
GTCATTATTG GGTCCTGGTT CTGGGAAGAA GAGGCCAGTC CGGAGGCAGT GGCAGGAGTC 
GGCTTTGAGT CAAAGCCTGG GACTGAGGAG GAAGAAATCA CTGTTGGGTC CTGGTTCTGG 
CCTGAAGAAG AAGCCAGTAT ACAGGCTGGA TCTCAGGCAG TAGAGGAAAT GGAGTCAGAG 
ACTGAAGAGG AAACCATTTT TGGGTCCTGG TTCTGGGATG GAAAAGAAGT CAGTGAAGAA 
GCAGGACCAT GCTGTGTATC CAAGCCAGAG GATGATGAAG AGATGATTGT TGAGTCCTGG 
TTCTGGTCTA GAGACAAAGC CATTAAGGAA ACTGGAACTG TGGCCACCTG TGAGTCCAAG 
CCAGAAAATG AGGAAGGGGC CATTGTTGGG TCTTGGTTTG AGGCTGAAGA TGAGGTAGAT 
AACAGGACTG ACAATGGAAG CAACTGTGGG TCCAGGACAT TAGCTGATGA AGATGAGGCC 
ATAGTGGGGT CCTGGTTCTG GGCAGGAGAT GAGGCCCATT TTGAATCAAA TCCTAGCCCC 
GTGTTCAGGG CCATTTGCAG GTCCACGTGT TCAGTTGAAC AGGAGCCTGA TCCTTCACGC 
AGGCCTCAGA GTTGGGAGGA GGTCACTGTT CAGTTCAAGC CTGGTCCATG GGGTAGGGTC 
GGCTTCCCAT CTATAAGCCC CTTTAGATTT CCGAAAGAGG CAGCATCTTT ATTCTGTGAA 
ATGTTTGGGG GCAAACCCAG GAACATGGTA CTTAGCCCAG AAGGGGAAGA TCAGGAATCT 
TTGCTTCAGC CTGATCAGCC TAGTCCTGAG TTCCCATTTC AGTATGATCC TTCCTACAGG 
TCAGTCCAGG AAATTCGAGA GCATCTTAGG GCCAAGGAGA GTACAGAGCC TGAGAGTTCA 
TCCTGTAACT GCATACAATG TGAGCTGAAA ATTGGTTCTG AAGAGTTTGA AGAACTCCTT 
TTATTAATGG AAAAAATTCG GGATCCTTTT ATTCATGAAA TATCTAAAAT CGCAATGGGT 
ATGAGAAGTG CTTCTCAATT TACCCGAGAT TTCATTCGAG ATTCAGGTGT TGTCTCACTT 
ATTGAAACCT TGCTTAATTA TCCGTCCTCC CGAGTTAGAA CAAGTTTTTT GGAAAATATG 
ATTCGCATGG CCCCACCTTA TCCGAATCTA AACATAATTC AGACATACAT ATGTAAAGTG 
TGTGAGGAAA CCCTTGCTTA TAGCGTGGAT TCCCCGGAAC AGCTGTCTGG AATAAGGATG 
ATTAGACATC TCACTACTAC TACTGACTAT CACACACTGG TTGCCAATTA TATGTCTGGG 
TTTCTCTCCT TATTAGCTAC AGGCAATGCC AAAACAAGGT TTCATGTTTT GAAAATGCTA 
CTGAATTTGT CTGAAAATCT TTTCATGACA AAAGAACTAC TCAGTGCTGA AGCAGTGTCA 
GAATTTATAG GCCTCTTTAA CAGGGAAGAG ACAAATGACA ATATTCAAAT TGTTCTTGCA 
ATATTTGAGA ATATTGGCAA CAATATCAAA AAAGAAACAG TGTTCTCTGA TGATGATTTC 
AATATTGAGC CGCTTATTTC TGCATTCCAC AAAGTTGAGA AATTTGCTAA GGAACTGCAA 
GGCAAAACAG ACAATCAAAA TGACCCTGAA GGGGACCAAG AAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GPRASP1 ( XM_017029982.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885471.1 Homo sapiens G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant X2, mRNA.

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CloneID	OHu13433
Clone ID Related Accession (Same CDS sequence)	NM_014710.5 , NM_001099411.1 , NM_001184727.1 , XM_017029981.1 , XM_017029982.1 , NM_014710.4 , NM_001184727.2 , NM_001099410.1
Accession Version	XM_017029982.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	G-protein coupled receptor-associated sorting protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGGGG CAGAGATTGA GTCTGGTGCC CAGGTCAAGC CTGAAAAGAA GCCTGGGGAA 
GAGGTTGTAG GTGGGGCTGA GATAGAGAAT GATGTCCCTC TGGTGGTCAG ACCCAAGGTT 
AGGACCCAGG CCCAGATAAT GCCTGGGGCA AGGCCCAAGA ATAAGTCCAA GGTTATGCCT 
GGAGCAAGCA CCAAAGTTGA GACAAGTGCA GTGGGTGGGG CACGCCCTAA GAGTAAGGCC 
AAGGCAATAC CTGTTTCACG ATTTAAGGAA GAAGCCCAGA TGTGGGCTCA GCCCAGGTTT 
GGTGCTGAAA GATTGTCTAA GACAGAGAGA AACTCCCAGA CCAATATCAT AGCCTCTCCA 
CTTGTCAGTA CTGATTCTGT CTTGGTTGCT AAAACAAAGT ACCTGTCTGA GGATAGAGAA 
CTGGTTAATA CAGACACTGA GAGCTTTCCT AGAAGGAAGG CCCATTACCA AGCAGGATTC 
CAGCCTTCTT TTAGGTCAAA GGAGGAGACC AATATGGGGT CCTGGTGCTG TCCTAGGCCT 
ACATCCAAAC AAGAAGCCTC TCCTAATTCT GATTTCAAAT GGGTAGACAA ATCTGTGAGT 
TCCTTGTTCT GGAGTGGAGA TGAGGTCACT GCAAAATTTC ATCCTGGGAA TAGGGTAAAA 
GACAGTAACA GATCCATGCA CATGGCCAAT CAAGAGGCTA ATACCATGTC TAGGTCCCAA 
ACTAACCAGG AGCTCTATAT TGCATCTAGT TCTGGTTCTG AGGATGAGTC TGTTAAGACA 
CCCTGGTTCT GGGCCAGAGA TAAAACCAAT ACCTGGTCTG GGCCCAGGGA AGATCCCAAT 
AGCAGGTCCA GGTTTAGGTC TAAGAAAGAA GTCTATGTTG AATCAAGTTC TGGATCTGAG 
CATGAAGACC ATTTGGAGTC CTGGTTTGGG GCTGGAAAGG AGGCCAAATT CAGGTCCAAA 
ATGAGAGCTG GGAAGGAGGC CAATAACAGG GCCAGGCACA GGGCCAAGCG AGAAGCTTGC 
ATTGATTTCA TGCCTGGGTC TATAGATGTA ATTAAAAAAG AGTCCTGTTT CTGGCCTGAA 
GAAAATGCTA ATACCTTTTC AAGGCCCATG ATCAAGAAAG AGGCCAGGGC CAGAGCAATG 
ACAAAGGAAG AGGCCAAAAC CAAGGCCCGA GCCAGGGCCA AGCAAGAAGC CAGGTCAGAG 
GAGGAAGCCC TCATTGGGAC CTGGTTCTGG GCTACAGACG AGTCCAGCAT GGCAGATGAA 
GCCAGCATAG AGTCCAGTCT ACAAGTGGAG GATGAGTCCA TAATTGGGAG TTGGTTCTGG 
ACTGAAGAAG AGGCCAGTAT GGGGACTGGG GCTAGCAGTA AATCCAGACC AAGGACTGAT 
GGGGAGCGTA TTGGTGATTC CTTATTTGGG GCTAGGGAAA AGACCAGTAT GAAAACTGGG 
GCTGAGGCCA CCTCTGAATC TATACTAGCA GCTGATGATG AACAGGTCAT TATTGGTTCC 
TGGTTCTGGG CTGGTGAAGA GGTCAACCAA GAGGCTGAGG AAGAGACCAT TTTTGGGTCG 
TGGTTCTGGG TCATTGATGC GGCCAGTGTG GAATCTGGTG TTGGGGTCAG CTGTGAGTCC 
AGGACAAGGT CTGAGGAAGA AGAGGTCATT GGTCCCTGGT TTTGGTCTGG AGAACAAGTT 
GATATAGAGG CTGGAATCGG AGAAGAGGCC AGGCCAGGAG CTGAAGAAGA GACAATATTC 
GGGTCCTGGT TTTGGGCTGA AAACCAGACC TATATGGATT GTAGGGCTGA AACTAGCTGT 
GACACCATGC AAGGGGCTGA GGAGGAGGAG CCCATTATTG GGTCCTGGTT TTGGACCAGA 
GTAGAAGCTT GTGTGGAGGG TGATGTCAAC AGCAAGTCTA GCCTGGAGGA CAAGGAAGAG 
GCCATGATAC CATGTTTTGG AGCCAAAGAA GAGGTCAGTA TGAAGCATGG GACTGGTGTC 
AGATGCAGAT TTATGGCAGG GGCTGAGGAG ACCAATAATA AGTCTTGCTT CTGGGCAGAA 
AAAGAACCCT GTATGTATCC TGCCGGTGGA GGAAGTTGGA AGTCTAGGCC AGAGGAGGAA 
GAGGACATTG TCAATTCGTG GTTCTGGTCC AGAAAATACA CAAAGCCAGA GGCCATTATA 
GGGTCCTGGT TATGGGCTAC AGAAGAGAGT AATATAGATG GGACTGGAGA AAAGGCCAAG 
TTACTGACTG AAGAGGAGAC CATAATCAAT TCCTGGTTCT GGAAAGAAGA TGAAGCCATT 
TCAGAGGCTA CTGACAGAGA AGAGTCCAGG CCAGAAGCTG AGGAGGGGGA CATTATTGGT 
TCTTGGTTCT GGGCTGGAGA AGAGGACAGA CTAGAGCCAG CTGCTGAGAC TAGAGAAGAA 
GACAGGCTAG CAGCTGAGAA AGAAGGTATT GTTGGGTCCT GGTTTGGGGC CAGAGAAGAG 
ACCATTAGAA GAGAGGCTGG GTCTTGCAGC AAATCCAGTC CTAAAGCTGA AGAGGAAGAA 
GTCATTATTG GGTCCTGGTT CTGGGAAGAA GAGGCCAGTC CGGAGGCAGT GGCAGGAGTC 
GGCTTTGAGT CAAAGCCTGG GACTGAGGAG GAAGAAATCA CTGTTGGGTC CTGGTTCTGG 
CCTGAAGAAG AAGCCAGTAT ACAGGCTGGA TCTCAGGCAG TAGAGGAAAT GGAGTCAGAG 
ACTGAAGAGG AAACCATTTT TGGGTCCTGG TTCTGGGATG GAAAAGAAGT CAGTGAAGAA 
GCAGGACCAT GCTGTGTATC CAAGCCAGAG GATGATGAAG AGATGATTGT TGAGTCCTGG 
TTCTGGTCTA GAGACAAAGC CATTAAGGAA ACTGGAACTG TGGCCACCTG TGAGTCCAAG 
CCAGAAAATG AGGAAGGGGC CATTGTTGGG TCTTGGTTTG AGGCTGAAGA TGAGGTAGAT 
AACAGGACTG ACAATGGAAG CAACTGTGGG TCCAGGACAT TAGCTGATGA AGATGAGGCC 
ATAGTGGGGT CCTGGTTCTG GGCAGGAGAT GAGGCCCATT TTGAATCAAA TCCTAGCCCC 
GTGTTCAGGG CCATTTGCAG GTCCACGTGT TCAGTTGAAC AGGAGCCTGA TCCTTCACGC 
AGGCCTCAGA GTTGGGAGGA GGTCACTGTT CAGTTCAAGC CTGGTCCATG GGGTAGGGTC 
GGCTTCCCAT CTATAAGCCC CTTTAGATTT CCGAAAGAGG CAGCATCTTT ATTCTGTGAA 
ATGTTTGGGG GCAAACCCAG GAACATGGTA CTTAGCCCAG AAGGGGAAGA TCAGGAATCT 
TTGCTTCAGC CTGATCAGCC TAGTCCTGAG TTCCCATTTC AGTATGATCC TTCCTACAGG 
TCAGTCCAGG AAATTCGAGA GCATCTTAGG GCCAAGGAGA GTACAGAGCC TGAGAGTTCA 
TCCTGTAACT GCATACAATG TGAGCTGAAA ATTGGTTCTG AAGAGTTTGA AGAACTCCTT 
TTATTAATGG AAAAAATTCG GGATCCTTTT ATTCATGAAA TATCTAAAAT CGCAATGGGT 
ATGAGAAGTG CTTCTCAATT TACCCGAGAT TTCATTCGAG ATTCAGGTGT TGTCTCACTT 
ATTGAAACCT TGCTTAATTA TCCGTCCTCC CGAGTTAGAA CAAGTTTTTT GGAAAATATG 
ATTCGCATGG CCCCACCTTA TCCGAATCTA AACATAATTC AGACATACAT ATGTAAAGTG 
TGTGAGGAAA CCCTTGCTTA TAGCGTGGAT TCCCCGGAAC AGCTGTCTGG AATAAGGATG 
ATTAGACATC TCACTACTAC TACTGACTAT CACACACTGG TTGCCAATTA TATGTCTGGG 
TTTCTCTCCT TATTAGCTAC AGGCAATGCC AAAACAAGGT TTCATGTTTT GAAAATGCTA 
CTGAATTTGT CTGAAAATCT TTTCATGACA AAAGAACTAC TCAGTGCTGA AGCAGTGTCA 
GAATTTATAG GCCTCTTTAA CAGGGAAGAG ACAAATGACA ATATTCAAAT TGTTCTTGCA 
ATATTTGAGA ATATTGGCAA CAATATCAAA AAAGAAACAG TGTTCTCTGA TGATGATTTC 
AATATTGAGC CGCTTATTTC TGCATTCCAC AAAGTTGAGA AATTTGCTAA GGAACTGCAA 
GGCAAAACAG ACAATCAAAA TGACCCTGAA GGGGACCAAG AAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885471.1
CDS	726..4913
Translation	MTGAEIESGAQVKPEKKPGEEVVGGAEIENDVPLVVRPKVRTQAQIMPGARPKNKSKVMPGASTKVETSAVGGARPKSKAKAIPVSRFKEEAQMWAQPRFGAERLSKTERNSQTNIIASPLVSTDSVLVAKTKYLSEDRELVNTDTESFPRRKAHYQAGFQPSFRSKEETNMGSWCCPRPTSKQEASPNSDFKWVDKSVSSLFWSGDEVTAKFHPGNRVKDSNRSMHMANQEANTMSRSQTNQELYIASSSGSEDESVKTPWFWARDKTNTWSGPREDPNSRSRFRSKKEVYVESSSGSEHEDHLESWFGAGKEAKFRSKMRAGKEANNRARHRAKREACIDFMPGSIDVIKKESCFWPEENANTFSRPMIKKEARARAMTKEEAKTKARARAKQEARSEEEALIGTWFWATDESSMADEASIESSLQVEDESIIGSWFWTEEEASMGTGASSKSRPRTDGERIGDSLFGAREKTSMKTGAEATSESILAADDEQVIIGSWFWAGEEVNQEAEEETIFGSWFWVIDAASVESGVGVSCESRTRSEEEEVIGPWFWSGEQVDIEAGIGEEARPGAEEETIFGSWFWAENQTYMDCRAETSCDTMQGAEEEEPIIGSWFWTRVEACVEGDVNSKSSLEDKEEAMIPCFGAKEEVSMKHGTGVRCRFMAGAEETNNKSCFWAEKEPCMYPAGGGSWKSRPEEEEDIVNSWFWSRKYTKPEAIIGSWLWATEESNIDGTGEKAKLLTEEETIINSWFWKEDEAISEATDREESRPEAEEGDIIGSWFWAGEEDRLEPAAETREEDRLAAEKEGIVGSWFGAREETIRREAGSCSKSSPKAEEEEVIIGSWFWEEEASPEAVAGVGFESKPGTEEEEITVGSWFWPEEEASIQAGSQAVEEMESETEEETIFGSWFWDGKEVSEEAGPCCVSKPEDDEEMIVESWFWSRDKAIKETGTVATCESKPENEEGAIVGSWFEAEDEVDNRTDNGSNCGSRTLADEDEAIVGSWFWAGDEAHFESNPSPVFRAICRSTCSVEQEPDPSRRPQSWEEVTVQFKPGPWGRVGFPSISPFRFPKEAASLFCEMFGGKPRNMVLSPEGEDQESLLQPDQPSPEFPFQYDPSYRSVQEIREHLRAKESTEPESSSCNCIQCELKIGSEEFEELLLLMEKIRDPFIHEISKIAMGMRSASQFTRDFIRDSGVVSLIETLLNYPSSRVRTSFLENMIRMAPPYPNLNIIQTYICKVCEETLAYSVDSPEQLSGIRMIRHLTTTTDYHTLVANYMSGFLSLLATGNAKTRFHVLKMLLNLSENLFMTKELLSAEAVSEFIGLFNREETNDNIQIVLAIFENIGNNIKKETVFSDDDFNIEPLISAFHKVEKFAKELQGKTDNQNDPEGDQEN

Target ORF information:

RefSeq Version	XM_017029982.1
Organism	Homo sapiens(human)
Definition	Homo sapiens G protein-coupled receptor associated sorting protein 1 (GPRASP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029982.1

ORF Insert Sequence:

ATGACTGGGG CAGAGATTGA GTCTGGTGCC CAGGTCAAGC CTGAAAAGAA GCCTGGGGAA 
GAGGTTGTAG GTGGGGCTGA GATAGAGAAT GATGTCCCTC TGGTGGTCAG ACCCAAGGTT 
AGGACCCAGG CCCAGATAAT GCCTGGGGCA AGGCCCAAGA ATAAGTCCAA GGTTATGCCT 
GGAGCAAGCA CCAAAGTTGA GACAAGTGCA GTGGGTGGGG CACGCCCTAA GAGTAAGGCC 
AAGGCAATAC CTGTTTCACG ATTTAAGGAA GAAGCCCAGA TGTGGGCTCA GCCCAGGTTT 
GGTGCTGAAA GATTGTCTAA GACAGAGAGA AACTCCCAGA CCAATATCAT AGCCTCTCCA 
CTTGTCAGTA CTGATTCTGT CTTGGTTGCT AAAACAAAGT ACCTGTCTGA GGATAGAGAA 
CTGGTTAATA CAGACACTGA GAGCTTTCCT AGAAGGAAGG CCCATTACCA AGCAGGATTC 
CAGCCTTCTT TTAGGTCAAA GGAGGAGACC AATATGGGGT CCTGGTGCTG TCCTAGGCCT 
ACATCCAAAC AAGAAGCCTC TCCTAATTCT GATTTCAAAT GGGTAGACAA ATCTGTGAGT 
TCCTTGTTCT GGAGTGGAGA TGAGGTCACT GCAAAATTTC ATCCTGGGAA TAGGGTAAAA 
GACAGTAACA GATCCATGCA CATGGCCAAT CAAGAGGCTA ATACCATGTC TAGGTCCCAA 
ACTAACCAGG AGCTCTATAT TGCATCTAGT TCTGGTTCTG AGGATGAGTC TGTTAAGACA 
CCCTGGTTCT GGGCCAGAGA TAAAACCAAT ACCTGGTCTG GGCCCAGGGA AGATCCCAAT 
AGCAGGTCCA GGTTTAGGTC TAAGAAAGAA GTCTATGTTG AATCAAGTTC TGGATCTGAG 
CATGAAGACC ATTTGGAGTC CTGGTTTGGG GCTGGAAAGG AGGCCAAATT CAGGTCCAAA 
ATGAGAGCTG GGAAGGAGGC CAATAACAGG GCCAGGCACA GGGCCAAGCG AGAAGCTTGC 
ATTGATTTCA TGCCTGGGTC TATAGATGTA ATTAAAAAAG AGTCCTGTTT CTGGCCTGAA 
GAAAATGCTA ATACCTTTTC AAGGCCCATG ATCAAGAAAG AGGCCAGGGC CAGAGCAATG 
ACAAAGGAAG AGGCCAAAAC CAAGGCCCGA GCCAGGGCCA AGCAAGAAGC CAGGTCAGAG 
GAGGAAGCCC TCATTGGGAC CTGGTTCTGG GCTACAGACG AGTCCAGCAT GGCAGATGAA 
GCCAGCATAG AGTCCAGTCT ACAAGTGGAG GATGAGTCCA TAATTGGGAG TTGGTTCTGG 
ACTGAAGAAG AGGCCAGTAT GGGGACTGGG GCTAGCAGTA AATCCAGACC AAGGACTGAT 
GGGGAGCGTA TTGGTGATTC CTTATTTGGG GCTAGGGAAA AGACCAGTAT GAAAACTGGG 
GCTGAGGCCA CCTCTGAATC TATACTAGCA GCTGATGATG AACAGGTCAT TATTGGTTCC 
TGGTTCTGGG CTGGTGAAGA GGTCAACCAA GAGGCTGAGG AAGAGACCAT TTTTGGGTCG 
TGGTTCTGGG TCATTGATGC GGCCAGTGTG GAATCTGGTG TTGGGGTCAG CTGTGAGTCC 
AGGACAAGGT CTGAGGAAGA AGAGGTCATT GGTCCCTGGT TTTGGTCTGG AGAACAAGTT 
GATATAGAGG CTGGAATCGG AGAAGAGGCC AGGCCAGGAG CTGAAGAAGA GACAATATTC 
GGGTCCTGGT TTTGGGCTGA AAACCAGACC TATATGGATT GTAGGGCTGA AACTAGCTGT 
GACACCATGC AAGGGGCTGA GGAGGAGGAG CCCATTATTG GGTCCTGGTT TTGGACCAGA 
GTAGAAGCTT GTGTGGAGGG TGATGTCAAC AGCAAGTCTA GCCTGGAGGA CAAGGAAGAG 
GCCATGATAC CATGTTTTGG AGCCAAAGAA GAGGTCAGTA TGAAGCATGG GACTGGTGTC 
AGATGCAGAT TTATGGCAGG GGCTGAGGAG ACCAATAATA AGTCTTGCTT CTGGGCAGAA 
AAAGAACCCT GTATGTATCC TGCCGGTGGA GGAAGTTGGA AGTCTAGGCC AGAGGAGGAA 
GAGGACATTG TCAATTCGTG GTTCTGGTCC AGAAAATACA CAAAGCCAGA GGCCATTATA 
GGGTCCTGGT TATGGGCTAC AGAAGAGAGT AATATAGATG GGACTGGAGA AAAGGCCAAG 
TTACTGACTG AAGAGGAGAC CATAATCAAT TCCTGGTTCT GGAAAGAAGA TGAAGCCATT 
TCAGAGGCTA CTGACAGAGA AGAGTCCAGG CCAGAAGCTG AGGAGGGGGA CATTATTGGT 
TCTTGGTTCT GGGCTGGAGA AGAGGACAGA CTAGAGCCAG CTGCTGAGAC TAGAGAAGAA 
GACAGGCTAG CAGCTGAGAA AGAAGGTATT GTTGGGTCCT GGTTTGGGGC CAGAGAAGAG 
ACCATTAGAA GAGAGGCTGG GTCTTGCAGC AAATCCAGTC CTAAAGCTGA AGAGGAAGAA 
GTCATTATTG GGTCCTGGTT CTGGGAAGAA GAGGCCAGTC CGGAGGCAGT GGCAGGAGTC 
GGCTTTGAGT CAAAGCCTGG GACTGAGGAG GAAGAAATCA CTGTTGGGTC CTGGTTCTGG 
CCTGAAGAAG AAGCCAGTAT ACAGGCTGGA TCTCAGGCAG TAGAGGAAAT GGAGTCAGAG 
ACTGAAGAGG AAACCATTTT TGGGTCCTGG TTCTGGGATG GAAAAGAAGT CAGTGAAGAA 
GCAGGACCAT GCTGTGTATC CAAGCCAGAG GATGATGAAG AGATGATTGT TGAGTCCTGG 
TTCTGGTCTA GAGACAAAGC CATTAAGGAA ACTGGAACTG TGGCCACCTG TGAGTCCAAG 
CCAGAAAATG AGGAAGGGGC CATTGTTGGG TCTTGGTTTG AGGCTGAAGA TGAGGTAGAT 
AACAGGACTG ACAATGGAAG CAACTGTGGG TCCAGGACAT TAGCTGATGA AGATGAGGCC 
ATAGTGGGGT CCTGGTTCTG GGCAGGAGAT GAGGCCCATT TTGAATCAAA TCCTAGCCCC 
GTGTTCAGGG CCATTTGCAG GTCCACGTGT TCAGTTGAAC AGGAGCCTGA TCCTTCACGC 
AGGCCTCAGA GTTGGGAGGA GGTCACTGTT CAGTTCAAGC CTGGTCCATG GGGTAGGGTC 
GGCTTCCCAT CTATAAGCCC CTTTAGATTT CCGAAAGAGG CAGCATCTTT ATTCTGTGAA 
ATGTTTGGGG GCAAACCCAG GAACATGGTA CTTAGCCCAG AAGGGGAAGA TCAGGAATCT 
TTGCTTCAGC CTGATCAGCC TAGTCCTGAG TTCCCATTTC AGTATGATCC TTCCTACAGG 
TCAGTCCAGG AAATTCGAGA GCATCTTAGG GCCAAGGAGA GTACAGAGCC TGAGAGTTCA 
TCCTGTAACT GCATACAATG TGAGCTGAAA ATTGGTTCTG AAGAGTTTGA AGAACTCCTT 
TTATTAATGG AAAAAATTCG GGATCCTTTT ATTCATGAAA TATCTAAAAT CGCAATGGGT 
ATGAGAAGTG CTTCTCAATT TACCCGAGAT TTCATTCGAG ATTCAGGTGT TGTCTCACTT 
ATTGAAACCT TGCTTAATTA TCCGTCCTCC CGAGTTAGAA CAAGTTTTTT GGAAAATATG 
ATTCGCATGG CCCCACCTTA TCCGAATCTA AACATAATTC AGACATACAT ATGTAAAGTG 
TGTGAGGAAA CCCTTGCTTA TAGCGTGGAT TCCCCGGAAC AGCTGTCTGG AATAAGGATG 
ATTAGACATC TCACTACTAC TACTGACTAT CACACACTGG TTGCCAATTA TATGTCTGGG 
TTTCTCTCCT TATTAGCTAC AGGCAATGCC AAAACAAGGT TTCATGTTTT GAAAATGCTA 
CTGAATTTGT CTGAAAATCT TTTCATGACA AAAGAACTAC TCAGTGCTGA AGCAGTGTCA 
GAATTTATAG GCCTCTTTAA CAGGGAAGAG ACAAATGACA ATATTCAAAT TGTTCTTGCA 
ATATTTGAGA ATATTGGCAA CAATATCAAA AAAGAAACAG TGTTCTCTGA TGATGATTTC 
AATATTGAGC CGCTTATTTC TGCATTCCAC AAAGTTGAGA AATTTGCTAA GGAACTGCAA 
GGCAAAACAG ACAATCAAAA TGACCCTGAA GGGGACCAAG AAAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Downregulation of 5-HT7 Serotonin Receptors by the Atypical Antipsychotics Clozapine and Olanzapine. Role of Motifs in the C-Terminal Domain and Interaction with GASP-1.
ACS chemical neuroscience6(7)1206-18(2015 Jul)
Manfra O,Van Craenenbroeck K,Skieterska K,Frimurer T,Schwartz TW,Levy FO,Andressen KW

Beclin 2 functions in autophagy, degradation of G protein-coupled receptors, and metabolism.
Cell154(5)1085-99(2013 Aug)
He C,Wei Y,Sun K,Li B,Dong X,Zou Z,Liu Y,Kinch LN,Khan S,Sinha S,Xavier RJ,Grishin NV,Xiao G,Eskelinen EL,Scherer PE,Whistler JL,Levine B

ARF6 and GASP-1 are post-endocytic sorting proteins selectively involved in the intracellular trafficking of dopamine D? receptors mediated by GRK and PKC in transfected cells.
British journal of pharmacology168(6)1355-74(2013 Mar)
Cho DI,Zheng M,Min C,Kwon KJ,Shin CY,Choi HK,Kim KM

G-protein coupled receptor-associated sorting protein 1 (GASP-1), a ubiquitous tumor marker.
Experimental and molecular pathology93(1)111-5(2012 Aug)
Zheng X,Chang F,Zhang X,Rothman VL,Tuszynski GP

Strength training with blood flow restriction diminishes myostatin gene expression.
Medicine and science in sports and exercise44(3)406-12(2012 Mar)
Laurentino GC,Ugrinowitsch C,Roschel H,Aoki MS,Soares AG,Neves M Jr,Aihara AY,Fernandes Ada R,Tricoli V

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GPRASP1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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