DIRAS3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	DIRAS3
Entrez Gene ID	9077
Full Name	DIRAS family GTPase 3
Synonyms	ARHI,NOEY2
General protein information	Preferred Names DIRAS family GTPase 3 Names GTP-binding protein Di-Ras3 DIRAS family, GTP-binding RAS-like 3 distinct subgroup of the Ras family member 3 ras homolog gene family, member I rho-related GTP-binding protein RhoI
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p31.3
Summary	This gene encodes a member of the ras superfamily. This gene is imprinted gene with monoallelic expression of the paternal allele which is associated with growth suppression. The encoded protein acts as a tumor suppressor whose function is abrogated in many ovarian and breast cancers. This protein may also play a role autophagy in certain cancer cells by regulating the autophagosome initiation complex. [provided by RefSeq, Nov 2015].
Disorder MIM:	605193

mRNA and Protein(s)

mRNA	Protein	Name
NM_004675.3	NP_004666.1	GTP-binding protein Di-Ras3 precursor
NM_004675.4	NP_004666.1	GTP-binding protein Di-Ras3 precursor
XM_017002773.1	XP_016858262.1	GTP-binding protein Di-Ras3 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	DIRAS3	NP_004666.1
Pan troglodytes (chimpanzee)	DIRAS3	XP_513477.1
	DIRAS3	XP_001095631.1
Bos taurus (cattle)	DIRAS3	NP_001029387.1

Gene Ontology
Bibliography

Related articles in PubMed

Long Noncoding RNA GAS5 Suppresses Cell Growth and Epithelial-Mesenchymal Transition in Osteosarcoma by Regulating the miR-221/ARHI Pathway.
Ye K, Wang S, Zhang H, Han H, Ma B, Nan W
Journal of cellular biochemistry118(12)4772-4781(2017 Dec)

Silencing of the small GTPase DIRAS3 induces cellular senescence in human white adipose stromal/progenitor cells.
Ejaz A, Mattesich M, Zwerschke W
Aging9(3)860-879(2017 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

lncRNA GAS5 functions as a competing endogenous RNA for miR-221 to suppress cell growth and EMT in osteosarcoma by regulating the miR-221/ARHI pathway.
Title: Long Noncoding RNA GAS5 Suppresses Cell Growth and Epithelial-Mesenchymal Transition in Osteosarcoma by Regulating the miR-221/ARHI Pathway.

We demonstrate that DIRAS3 knock-down (KD) in adipose stromal/progenitor cells (ASCs) induces activation of Akt-mTOR signaling and proliferation arrest. DIRAS3 KD ASCs lose the potential to form colonies and are negative for Ki-67. Moreover, silencing of DIRAS3 results in a premature senescence phenotype.
Title: Silencing of the small GTPase DIRAS3 induces cellular senescence in human white adipose stromal/progenitor cells.

The overexpression of ARHI promotes colon cancer SW480 cell apoptosis by inhibiting the level of Akt.
Title: [Overexpression of aplysia Ras homolog I (ARHI) increases apoptosis in colon cancer SW480 cells].

DiRas3 binds to KSR1 independently of its interaction with activated Ras and RAF.
Title: Stabilization of C-RAF:KSR1 complex by DiRas3 reduces availability of C-RAF for dimerization with B-RAF.

ARHI-mediated up-regulation of glycolysis and glutaminolysis was found to be autophagy-dependent and inhibition of these metabolic pathways resulted in decreased cell viability.
Title: Induction of autophagy by ARHI (DIRAS3) alters fundamental metabolic pathways in ovarian cancer models.

The following DIRAS3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DIRAS3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17916	NM_004675.4 Latest version!	Homo sapiens DIRAS family GTPase 3 (DIRAS3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu17916	XM_017002773.1 Latest version!	Homo sapiens DIRAS family GTPase 3 (DIRAS3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
View Old Accession Versions >>
OHu17916	NM_004675.3	Homo sapiens DIRAS family GTPase 3 (DIRAS3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DIRAS3 ( NM_004675.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004666.1 Homo sapiens DIRAS family GTPase 3 (DIRAS3), mRNA.

Price & Availability↑

CloneID	OHu17916
Clone ID Related Accession (Same CDS sequence)	NM_004675.3 , NM_004675.4 , XM_017002773.1
Accession Version	NM_004675.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	GTP-binding protein Di-Ras3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK096393.1 and U96750.1. This sequence is a reference standard in the RefSeqGene project. On Nov 6, 2015 this sequence version replaced NM_004675.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK096393.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 9874798 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGTAACG CCAGCTTTGG CTCCAAGGAA CAGAAGCTGC TGAAGCGGTT GCGGCTTCTG 
CCCGCCCTGC TTATCCTCCG CGCCTTCAAG CCCCACAGGA AGATCAGAGA TTACCGCGTC 
GTGGTAGTCG GCACCGCTGG TGTGGGGAAA AGTACGCTGC TGCACAAGTG GGCGAGCGGC 
AACTTCCGTC ATGAGTACCT GCCGACCATT GAAAATACCT ACTGCCAGTT GCTGGGCTGC 
AGCCACGGTG TGCTTTCCCT GCACATCACC GACAGCAAGA GTGGCGACGG CAACCGCGCT 
CTGCAGCGCC ACGTTATAGC CCGGGGCCAC GCCTTCGTCC TGGTCTACTC AGTCACCAAG 
AAGGAAACCC TGGAAGAGCT GAAGGCCTTC TATGAGCTGA TCTGCAAGAT CAAAGGTAAC 
AACCTGCATA AGTTCCCCAT CGTGCTGGTG GGCAATAAAA GTGATGACAC CCACCGGGAG 
GTGGCCCTGA ATGATGGTGC CACCTGTGCG ATGGAGTGGA ATTGCGCCTT CATGGAGATT 
TCAGCCAAGA CCGATGTGAA TGTGCAGGAG CTGTTCCACA TGCTGCTGAA TTACAAGAAA 
AAGCCCACCA CCGGCCTCCA GGAGCCCGAG AAGAAATCCC AGATGCCCAA CACCACTGAG 
AAGCTGCTTG ACAAGTGCAT AATCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004666.1
CDS	638..1327
Translation	MGNASFGSKEQKLLKRLRLLPALLILRAFKPHRKIRDYRVVVVGTAGVGKSTLLHKWASGNFRHEYLPTIENTYCQLLGCSHGVLSLHITDSKSGDGNRALQRHVIARGHAFVLVYSVTKKETLEELKAFYELICKIKGNNLHKFPIVLVGNKSDDTHREVALNDGATCAMEWNCAFMEISAKTDVNVQELFHMLLNYKKKPTTGLQEPEKKSQMPNTTEKLLDKCIIM

Target ORF information:

RefSeq Version	NM_004675.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DIRAS family GTPase 3 (DIRAS3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004675.3

ORF Insert Sequence:

ATGGGTAACG CCAGCTTTGG CTCCAAGGAA CAGAAGCTGC TGAAGCGGTT GCGGCTTCTG 
CCCGCCCTGC TTATCCTCCG CGCCTTCAAG CCCCACAGGA AGATCAGAGA TTACCGCGTC 
GTGGTAGTCG GCACCGCTGG TGTGGGGAAA AGTACGCTGC TGCACAAGTG GGCGAGCGGC 
AACTTCCGTC ATGAGTACCT GCCGACCATT GAAAATACCT ACTGCCAGTT GCTGGGCTGC 
AGCCACGGTG TGCTTTCCCT GCACATCACC GACAGCAAGA GTGGCGACGG CAACCGCGCT 
CTGCAGCGCC ACGTTATAGC CCGGGGCCAC GCCTTCGTCC TGGTCTACTC AGTCACCAAG 
AAGGAAACCC TGGAAGAGCT GAAGGCCTTC TATGAGCTGA TCTGCAAGAT CAAAGGTAAC 
AACCTGCATA AGTTCCCCAT CGTGCTGGTG GGCAATAAAA GTGATGACAC CCACCGGGAG 
GTGGCCCTGA ATGATGGTGC CACCTGTGCG ATGGAGTGGA ATTGCGCCTT CATGGAGATT 
TCAGCCAAGA CCGATGTGAA TGTGCAGGAG CTGTTCCACA TGCTGCTGAA TTACAAGAAA 
AAGCCCACCA CCGGCCTCCA GGAGCCCGAG AAGAAATCCC AGATGCCCAA CACCACTGAG 
AAGCTGCTTG ACAAGTGCAT AATCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIRAS3 ( NM_004675.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004666.1 Homo sapiens DIRAS family GTPase 3 (DIRAS3), mRNA.

Price & Availability↑

CloneID	OHu17916
Clone ID Related Accession (Same CDS sequence)	NM_004675.3 , NM_004675.4 , XM_017002773.1
Accession Version	NM_004675.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Homo sapiens(human)
Product	GTP-binding protein Di-Ras3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK096393.1, U96750.1 and AL157407.11. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_004675.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK096393.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1966682, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## imprinted gene :: PMID: 9874798 MANE Ensembl match :: ENST00000370981.3/ ENSP00000360020.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGTAACG CCAGCTTTGG CTCCAAGGAA CAGAAGCTGC TGAAGCGGTT GCGGCTTCTG 
CCCGCCCTGC TTATCCTCCG CGCCTTCAAG CCCCACAGGA AGATCAGAGA TTACCGCGTC 
GTGGTAGTCG GCACCGCTGG TGTGGGGAAA AGTACGCTGC TGCACAAGTG GGCGAGCGGC 
AACTTCCGTC ATGAGTACCT GCCGACCATT GAAAATACCT ACTGCCAGTT GCTGGGCTGC 
AGCCACGGTG TGCTTTCCCT GCACATCACC GACAGCAAGA GTGGCGACGG CAACCGCGCT 
CTGCAGCGCC ACGTTATAGC CCGGGGCCAC GCCTTCGTCC TGGTCTACTC AGTCACCAAG 
AAGGAAACCC TGGAAGAGCT GAAGGCCTTC TATGAGCTGA TCTGCAAGAT CAAAGGTAAC 
AACCTGCATA AGTTCCCCAT CGTGCTGGTG GGCAATAAAA GTGATGACAC CCACCGGGAG 
GTGGCCCTGA ATGATGGTGC CACCTGTGCG ATGGAGTGGA ATTGCGCCTT CATGGAGATT 
TCAGCCAAGA CCGATGTGAA TGTGCAGGAG CTGTTCCACA TGCTGCTGAA TTACAAGAAA 
AAGCCCACCA CCGGCCTCCA GGAGCCCGAG AAGAAATCCC AGATGCCCAA CACCACTGAG 
AAGCTGCTTG ACAAGTGCAT AATCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004666.1
CDS	638..1327
Translation	MGNASFGSKEQKLLKRLRLLPALLILRAFKPHRKIRDYRVVVVGTAGVGKSTLLHKWASGNFRHEYLPTIENTYCQLLGCSHGVLSLHITDSKSGDGNRALQRHVIARGHAFVLVYSVTKKETLEELKAFYELICKIKGNNLHKFPIVLVGNKSDDTHREVALNDGATCAMEWNCAFMEISAKTDVNVQELFHMLLNYKKKPTTGLQEPEKKSQMPNTTEKLLDKCIIM

Target ORF information:

RefSeq Version	NM_004675.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DIRAS family GTPase 3 (DIRAS3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004675.4

ORF Insert Sequence:

ATGGGTAACG CCAGCTTTGG CTCCAAGGAA CAGAAGCTGC TGAAGCGGTT GCGGCTTCTG 
CCCGCCCTGC TTATCCTCCG CGCCTTCAAG CCCCACAGGA AGATCAGAGA TTACCGCGTC 
GTGGTAGTCG GCACCGCTGG TGTGGGGAAA AGTACGCTGC TGCACAAGTG GGCGAGCGGC 
AACTTCCGTC ATGAGTACCT GCCGACCATT GAAAATACCT ACTGCCAGTT GCTGGGCTGC 
AGCCACGGTG TGCTTTCCCT GCACATCACC GACAGCAAGA GTGGCGACGG CAACCGCGCT 
CTGCAGCGCC ACGTTATAGC CCGGGGCCAC GCCTTCGTCC TGGTCTACTC AGTCACCAAG 
AAGGAAACCC TGGAAGAGCT GAAGGCCTTC TATGAGCTGA TCTGCAAGAT CAAAGGTAAC 
AACCTGCATA AGTTCCCCAT CGTGCTGGTG GGCAATAAAA GTGATGACAC CCACCGGGAG 
GTGGCCCTGA ATGATGGTGC CACCTGTGCG ATGGAGTGGA ATTGCGCCTT CATGGAGATT 
TCAGCCAAGA CCGATGTGAA TGTGCAGGAG CTGTTCCACA TGCTGCTGAA TTACAAGAAA 
AAGCCCACCA CCGGCCTCCA GGAGCCCGAG AAGAAATCCC AGATGCCCAA CACCACTGAG 
AAGCTGCTTG ACAAGTGCAT AATCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DIRAS3 ( XM_017002773.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858262.1 Homo sapiens DIRAS family GTPase 3 (DIRAS3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu17916
Clone ID Related Accession (Same CDS sequence)	NM_004675.3 , NM_004675.4 , XM_017002773.1
Accession Version	XM_017002773.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 690bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	GTP-binding protein Di-Ras3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTAACG CCAGCTTTGG CTCCAAGGAA CAGAAGCTGC TGAAGCGGTT GCGGCTTCTG 
CCCGCCCTGC TTATCCTCCG CGCCTTCAAG CCCCACAGGA AGATCAGAGA TTACCGCGTC 
GTGGTAGTCG GCACCGCTGG TGTGGGGAAA AGTACGCTGC TGCACAAGTG GGCGAGCGGC 
AACTTCCGTC ATGAGTACCT GCCGACCATT GAAAATACCT ACTGCCAGTT GCTGGGCTGC 
AGCCACGGTG TGCTTTCCCT GCACATCACC GACAGCAAGA GTGGCGACGG CAACCGCGCT 
CTGCAGCGCC ACGTTATAGC CCGGGGCCAC GCCTTCGTCC TGGTCTACTC AGTCACCAAG 
AAGGAAACCC TGGAAGAGCT GAAGGCCTTC TATGAGCTGA TCTGCAAGAT CAAAGGTAAC 
AACCTGCATA AGTTCCCCAT CGTGCTGGTG GGCAATAAAA GTGATGACAC CCACCGGGAG 
GTGGCCCTGA ATGATGGTGC CACCTGTGCG ATGGAGTGGA ATTGCGCCTT CATGGAGATT 
TCAGCCAAGA CCGATGTGAA TGTGCAGGAG CTGTTCCACA TGCTGCTGAA TTACAAGAAA 
AAGCCCACCA CCGGCCTCCA GGAGCCCGAG AAGAAATCCC AGATGCCCAA CACCACTGAG 
AAGCTGCTTG ACAAGTGCAT AATCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858262.1
CDS	1281..1970
Translation	MGNASFGSKEQKLLKRLRLLPALLILRAFKPHRKIRDYRVVVVGTAGVGKSTLLHKWASGNFRHEYLPTIENTYCQLLGCSHGVLSLHITDSKSGDGNRALQRHVIARGHAFVLVYSVTKKETLEELKAFYELICKIKGNNLHKFPIVLVGNKSDDTHREVALNDGATCAMEWNCAFMEISAKTDVNVQELFHMLLNYKKKPTTGLQEPEKKSQMPNTTEKLLDKCIIM

Target ORF information:

RefSeq Version	XM_017002773.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DIRAS family GTPase 3 (DIRAS3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002773.1

ORF Insert Sequence:

ATGGGTAACG CCAGCTTTGG CTCCAAGGAA CAGAAGCTGC TGAAGCGGTT GCGGCTTCTG 
CCCGCCCTGC TTATCCTCCG CGCCTTCAAG CCCCACAGGA AGATCAGAGA TTACCGCGTC 
GTGGTAGTCG GCACCGCTGG TGTGGGGAAA AGTACGCTGC TGCACAAGTG GGCGAGCGGC 
AACTTCCGTC ATGAGTACCT GCCGACCATT GAAAATACCT ACTGCCAGTT GCTGGGCTGC 
AGCCACGGTG TGCTTTCCCT GCACATCACC GACAGCAAGA GTGGCGACGG CAACCGCGCT 
CTGCAGCGCC ACGTTATAGC CCGGGGCCAC GCCTTCGTCC TGGTCTACTC AGTCACCAAG 
AAGGAAACCC TGGAAGAGCT GAAGGCCTTC TATGAGCTGA TCTGCAAGAT CAAAGGTAAC 
AACCTGCATA AGTTCCCCAT CGTGCTGGTG GGCAATAAAA GTGATGACAC CCACCGGGAG 
GTGGCCCTGA ATGATGGTGC CACCTGTGCG ATGGAGTGGA ATTGCGCCTT CATGGAGATT 
TCAGCCAAGA CCGATGTGAA TGTGCAGGAG CTGTTCCACA TGCTGCTGAA TTACAAGAAA 
AAGCCCACCA CCGGCCTCCA GGAGCCCGAG AAGAAATCCC AGATGCCCAA CACCACTGAG 
AAGCTGCTTG ACAAGTGCAT AATCATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Long Noncoding RNA GAS5 Suppresses Cell Growth and Epithelial-Mesenchymal Transition in Osteosarcoma by Regulating the miR-221/ARHI Pathway.
Journal of cellular biochemistry118(12)4772-4781(2017 Dec)
Ye K,Wang S,Zhang H,Han H,Ma B,Nan W

Silencing of the small GTPase DIRAS3 induces cellular senescence in human white adipose stromal/progenitor cells.
Aging9(3)860-879(2017 Mar)
Ejaz A,Mattesich M,Zwerschke W

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DIRAS3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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