TNFSF12 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TNFSF12
Entrez Gene ID	8742
Full Name	TNF superfamily member 12
Synonyms	APO3L,DR3LG,TNLG4A,TWEAK
General protein information	Preferred Names TNF superfamily member 12 Names tumor necrosis factor ligand superfamily member 12 APO3 ligand APO3/DR3 ligand TNF-related WEAK inducer of apoptosis tumor necrosis factor (ligand) superfamily, member 12 tumor necrosis factor ligand 4A tumor necrosis factor superfamily member 12
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17p13.1
Summary	The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is a ligand for the FN14/TWEAKR receptor. This cytokine has overlapping signaling functions with TNF, but displays a much wider tissue distribution. This cytokine, which exists in both membrane-bound and secreted forms, can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. This cytokine is also found to promote proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. Alternative splicing results in multiple transcript variants. Some transcripts skip the last exon of this gene and continue into the second exon of the neighboring TNFSF13 gene; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13. [provided by RefSeq, Oct 2010].
Disorder MIM:	602695

mRNA and Protein(s)

mRNA	Protein	Name
NM_003809.2	NP_003800.1	tumor necrosis factor ligand superfamily member 12 proprotein
NM_003809.3	NP_003800.1	tumor necrosis factor ligand superfamily member 12 proprotein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TNFSF12	NP_001192058.1
Rattus norvegicus (Norway rat)	Tnfsf12	NP_001001513.2
Danio rerio (zebrafish)	tnfsf12	NP_001070075.1
Canis lupus familiaris (dog)	TNFSF12	NP_001192097.1
Mus musculus (house mouse)	Tnfsf12	NP_035744.1
Homo sapiens (human)	TNFSF12	NP_003800.1
Bos taurus (cattle)	TNFSF12	NP_001192072.1

Gene Ontology
Bibliography

Related articles in PubMed

Fn14?TRAIL fusion protein is oligomerized by TWEAK into a superefficient TRAIL analog.
Prigozhina TB, Szafer F, Aronin A, Tzdaka K, Amsili S, Makdasi E, Shani N, Dranitzki Elhalel M
Cancer letters40099-109(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Fn14.TRAIL can be converted into a highly effective TRAIL oligomer upon binding to TWEAK which induces lymphoblast apoptosis.
Title: Fn14?TRAIL fusion protein is oligomerized by TWEAK into a superefficient TRAIL analog.

TWEAK may contribute to the pathogenesis of BP by reducing BP180 expression and cellular adherence, involving the activation of ERK and NF-kappaB pathways. TWEAK may serve as a biomarker or therapeutic target of BP.
Title: TWEAK/Fn14 Activation Contributes to the Pathogenesis of Bullous Pemphigoid.

TWEAK upregulated the expression of Fn14.
Title: TWEAK/Fn14 promotes pro-inflammatory cytokine secretion in hepatic stellate cells via NF-?B/STAT3 pathways.

Plasma TWEAK levels do not reflect disease activity or the grade of inflammation in patients with newly diagnosed inflammatory bowel disease.
Title: TWEAK is not elevated in patients with newly diagnosed inflammatory bowel disease.

Data show that aurintricarboxylic acid (ATA) targets the TNF-related WEAK inducer of apoptosis (TWEAK)-fibroblast growth factor-inducible 14 (Fn14) signaling axis, which could potentially be developed as a new therapeutic agent for treatment of glioblastoma (GBM) patients.
Title: Identification of aurintricarboxylic acid as a selective inhibitor of the TWEAK-Fn14 signaling pathway in glioblastoma cells.

The following TNFSF12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TNFSF12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16549	NM_003809.3 Latest version!	Homo sapiens TNF superfamily member 12 (TNFSF12), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$134.50-$188.30 $269.00
View Old Accession Versions >>
OHu16549	NM_003809.2	Homo sapiens TNF superfamily member 12 (TNFSF12), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$134.50-$188.30 $269.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TNFSF12 ( NM_003809.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_003800.1 Homo sapiens TNF superfamily member 12 (TNFSF12), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu16549
Clone ID Related Accession (Same CDS sequence)	NM_003809.2 , NM_003809.3
Accession Version	NM_003809.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-17
Organism	Homo sapiens(human)
Product	tumor necrosis factor ligand superfamily member 12 proprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI091441.1, BC104420.1, AF030099.1 and AF055872.1. This sequence is a reference standard in the RefSeqGene project. On Oct 7, 2002 this sequence version replaced NM_003809.1. Transcript Variant: This variant (1) represents the shorter transcript but encodes the functional protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF055872.1, ERR279848.9170.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGCCC GTCGGAGCCA GAGGCGGAGG GGGCGCCGGG GGGAGCCGGG CACCGCCCTG 
CTGGTCCCGC TCGCGCTGGG CCTGGGCCTG GCGCTGGCCT GCCTCGGCCT CCTGCTGGCC 
GTGGTCAGTT TGGGGAGCCG GGCATCGCTG TCCGCCCAGG AGCCTGCCCA GGAGGAGCTG 
GTGGCAGAGG AGGACCAGGA CCCGTCGGAA CTGAATCCCC AGACAGAAGA AAGCCAGGAT 
CCTGCGCCTT TCCTGAACCG ACTAGTTCGG CCTCGCAGAA GTGCACCTAA AGGCCGGAAA 
ACACGGGCTC GAAGAGCGAT CGCAGCCCAT TATGAAGTTC ATCCACGACC TGGACAGGAC 
GGAGCGCAGG CAGGTGTGGA CGGGACAGTG AGTGGCTGGG AGGAAGCCAG AATCAACAGC 
TCCAGCCCTC TGCGCTACAA CCGCCAGATC GGGGAGTTTA TAGTCACCCG GGCTGGGCTC 
TACTACCTGT ACTGTCAGGT GCACTTTGAT GAGGGGAAGG CTGTCTACCT GAAGCTGGAC 
TTGCTGGTGG ATGGTGTGCT GGCCCTGCGC TGCCTGGAGG AATTCTCAGC CACTGCGGCG 
AGTTCCCTCG GGCCCCAGCT CCGCCTCTGC CAGGTGTCTG GGCTGTTGGC CCTGCGGCCA 
GGGTCCTCCC TGCGGATCCG CACCCTCCCC TGGGCCCATC TCAAGGCTGC CCCCTTCCTC 
ACCTACTTCG GACTCTTCCA GGTTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003800.1
CDS	97..846
Translation	MAARRSQRRRGRRGEPGTALLVPLALGLGLALACLGLLLAVVSLGSRASLSAQEPAQEELVAEEDQDPSELNPQTEESQDPAPFLNRLVRPRRSAPKGRKTRARRAIAAHYEVHPRPGQDGAQAGVDGTVSGWEEARINSSSPLRYNRQIGEFIVTRAGLYYLYCQVHFDEGKAVYLKLDLLVDGVLALRCLEEFSATAASSLGPQLRLCQVSGLLALRPGSSLRIRTLPWAHLKAAPFLTYFGLFQVH

Target ORF information:

RefSeq Version	NM_003809.2
Organism	Homo sapiens(human)
Definition	Homo sapiens TNF superfamily member 12 (TNFSF12), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003809.2

ORF Insert Sequence:

ATGGCCGCCC GTCGGAGCCA GAGGCGGAGG GGGCGCCGGG GGGAGCCGGG CACCGCCCTG 
CTGGTCCCGC TCGCGCTGGG CCTGGGCCTG GCGCTGGCCT GCCTCGGCCT CCTGCTGGCC 
GTGGTCAGTT TGGGGAGCCG GGCATCGCTG TCCGCCCAGG AGCCTGCCCA GGAGGAGCTG 
GTGGCAGAGG AGGACCAGGA CCCGTCGGAA CTGAATCCCC AGACAGAAGA AAGCCAGGAT 
CCTGCGCCTT TCCTGAACCG ACTAGTTCGG CCTCGCAGAA GTGCACCTAA AGGCCGGAAA 
ACACGGGCTC GAAGAGCGAT CGCAGCCCAT TATGAAGTTC ATCCACGACC TGGACAGGAC 
GGAGCGCAGG CAGGTGTGGA CGGGACAGTG AGTGGCTGGG AGGAAGCCAG AATCAACAGC 
TCCAGCCCTC TGCGCTACAA CCGCCAGATC GGGGAGTTTA TAGTCACCCG GGCTGGGCTC 
TACTACCTGT ACTGTCAGGT GCACTTTGAT GAGGGGAAGG CTGTCTACCT GAAGCTGGAC 
TTGCTGGTGG ATGGTGTGCT GGCCCTGCGC TGCCTGGAGG AATTCTCAGC CACTGCGGCG 
AGTTCCCTCG GGCCCCAGCT CCGCCTCTGC CAGGTGTCTG GGCTGTTGGC CCTGCGGCCA 
GGGTCCTCCC TGCGGATCCG CACCCTCCCC TGGGCCCATC TCAAGGCTGC CCCCTTCCTC 
ACCTACTTCG GACTCTTCCA GGTTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNFSF12 ( NM_003809.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003800.1 Homo sapiens TNF superfamily member 12 (TNFSF12), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu16549
Clone ID Related Accession (Same CDS sequence)	NM_003809.2 , NM_003809.3
Accession Version	NM_003809.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	tumor necrosis factor ligand superfamily member 12 proprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AI091441.1, BC104420.1 and AF030099.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_003809.2. Transcript Variant: This variant (1) represents the shorter transcript but encodes the functional protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF055872.1, AY358870.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000293825.11/ ENSP00000293825.6 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCGCCC GTCGGAGCCA GAGGCGGAGG GGGCGCCGGG GGGAGCCGGG CACCGCCCTG 
CTGGTCCCGC TCGCGCTGGG CCTGGGCCTG GCGCTGGCCT GCCTCGGCCT CCTGCTGGCC 
GTGGTCAGTT TGGGGAGCCG GGCATCGCTG TCCGCCCAGG AGCCTGCCCA GGAGGAGCTG 
GTGGCAGAGG AGGACCAGGA CCCGTCGGAA CTGAATCCCC AGACAGAAGA AAGCCAGGAT 
CCTGCGCCTT TCCTGAACCG ACTAGTTCGG CCTCGCAGAA GTGCACCTAA AGGCCGGAAA 
ACACGGGCTC GAAGAGCGAT CGCAGCCCAT TATGAAGTTC ATCCACGACC TGGACAGGAC 
GGAGCGCAGG CAGGTGTGGA CGGGACAGTG AGTGGCTGGG AGGAAGCCAG AATCAACAGC 
TCCAGCCCTC TGCGCTACAA CCGCCAGATC GGGGAGTTTA TAGTCACCCG GGCTGGGCTC 
TACTACCTGT ACTGTCAGGT GCACTTTGAT GAGGGGAAGG CTGTCTACCT GAAGCTGGAC 
TTGCTGGTGG ATGGTGTGCT GGCCCTGCGC TGCCTGGAGG AATTCTCAGC CACTGCGGCG 
AGTTCCCTCG GGCCCCAGCT CCGCCTCTGC CAGGTGTCTG GGCTGTTGGC CCTGCGGCCA 
GGGTCCTCCC TGCGGATCCG CACCCTCCCC TGGGCCCATC TCAAGGCTGC CCCCTTCCTC 
ACCTACTTCG GACTCTTCCA GGTTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003800.1
CDS	97..846
Translation	MAARRSQRRRGRRGEPGTALLVPLALGLGLALACLGLLLAVVSLGSRASLSAQEPAQEELVAEEDQDPSELNPQTEESQDPAPFLNRLVRPRRSAPKGRKTRARRAIAAHYEVHPRPGQDGAQAGVDGTVSGWEEARINSSSPLRYNRQIGEFIVTRAGLYYLYCQVHFDEGKAVYLKLDLLVDGVLALRCLEEFSATAASSLGPQLRLCQVSGLLALRPGSSLRIRTLPWAHLKAAPFLTYFGLFQVH

Target ORF information:

RefSeq Version	NM_003809.3
Organism	Homo sapiens(human)
Definition	Homo sapiens TNF superfamily member 12 (TNFSF12), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003809.3

ORF Insert Sequence:

ATGGCCGCCC GTCGGAGCCA GAGGCGGAGG GGGCGCCGGG GGGAGCCGGG CACCGCCCTG 
CTGGTCCCGC TCGCGCTGGG CCTGGGCCTG GCGCTGGCCT GCCTCGGCCT CCTGCTGGCC 
GTGGTCAGTT TGGGGAGCCG GGCATCGCTG TCCGCCCAGG AGCCTGCCCA GGAGGAGCTG 
GTGGCAGAGG AGGACCAGGA CCCGTCGGAA CTGAATCCCC AGACAGAAGA AAGCCAGGAT 
CCTGCGCCTT TCCTGAACCG ACTAGTTCGG CCTCGCAGAA GTGCACCTAA AGGCCGGAAA 
ACACGGGCTC GAAGAGCGAT CGCAGCCCAT TATGAAGTTC ATCCACGACC TGGACAGGAC 
GGAGCGCAGG CAGGTGTGGA CGGGACAGTG AGTGGCTGGG AGGAAGCCAG AATCAACAGC 
TCCAGCCCTC TGCGCTACAA CCGCCAGATC GGGGAGTTTA TAGTCACCCG GGCTGGGCTC 
TACTACCTGT ACTGTCAGGT GCACTTTGAT GAGGGGAAGG CTGTCTACCT GAAGCTGGAC 
TTGCTGGTGG ATGGTGTGCT GGCCCTGCGC TGCCTGGAGG AATTCTCAGC CACTGCGGCG 
AGTTCCCTCG GGCCCCAGCT CCGCCTCTGC CAGGTGTCTG GGCTGTTGGC CCTGCGGCCA 
GGGTCCTCCC TGCGGATCCG CACCCTCCCC TGGGCCCATC TCAAGGCTGC CCCCTTCCTC 
ACCTACTTCG GACTCTTCCA GGTTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Fn14?TRAIL fusion protein is oligomerized by TWEAK into a superefficient TRAIL analog.
Cancer letters40099-109(2017 Aug)
Prigozhina TB,Szafer F,Aronin A,Tzdaka K,Amsili S,Makdasi E,Shani N,Dranitzki Elhalel M

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TNFSF12 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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