CST7 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CST7
Entrez Gene ID	8530
Full Name	cystatin F
Synonyms	CMAP
General protein information	Preferred Names cystatin F Names cystatin-F cystatin-7 cystatin-like metastasis-associated protein leukocystatin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20p11.21
Summary	The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. This gene encodes a glycosylated cysteine protease inhibitor with a putative role in immune regulation through inhibition of a unique target in the hematopoietic system. Expression of the protein has been observed in various human cancer cell lines established from malignant tumors. [provided by RefSeq, Jul 2008].
Disorder MIM:	603253

mRNA and Protein(s)

mRNA	Protein	Name
NM_003650.4	NP_003641.3	cystatin-F precursor
NM_003650.3	NP_003641.3	cystatin-F precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Cst7	NP_001099993.1
	CST7	XP_001098076.1
Bos taurus (cattle)	CST7	XP_002692220.1
Canis lupus familiaris (dog)	CST7	XP_005634686.1
Homo sapiens (human)	CST7	NP_003641.3
Pan troglodytes (chimpanzee)	CST7	XP_003316900.1
Mus musculus (house mouse)	Cst7	NP_034107.2
Gallus gallus (chicken)	CST7	NP_001186323.1
Xenopus tropicalis (tropical clawed frog)	cst7	NP_001011317.1

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of cathepsins S and L by cystatin F during maturation of dendritic cells.
Magister S, Obermajer N, Mirkovi? B, Svajger U, Renko M, Softi? A, Romih R, Colbert JD, Watts C, Kos J
European journal of cell biology91(5)391-401(2012 May)

Microglial cystatin F expression is a sensitive indicator for ongoing demyelination with concurrent remyelination.
Ma J, Tanaka KF, Shimizu T, Bernard CC, Kakita A, Takahashi H, Pfeiffer SE, Ikenaka K
Journal of neuroscience research89(5)639-49(2011 May)

Glycosylation directs targeting and activation of cystatin f from intracellular and extracellular sources.
Colbert JD, Plechanovov? A, Watts C
Traffic (Copenhagen, Denmark)10(4)425-37(2009 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Regulation of cathepsins S and L by cystatin F during maturation of dendritic cells.
Title: Regulation of cathepsins S and L by cystatin F during maturation of dendritic cells.

Transgenic cystatin F is expressed in model mice only during demyelination; in chronic demyelination, remyelinating ability is lost.
Title: Microglial cystatin F expression is a sensitive indicator for ongoing demyelination with concurrent remyelination.

Data demonstrate the addition of N-linked sugars to an Asn-X-Cys motif in cystatin F and suggest that the mannose 6-phosphate sorting machinery is used to divert cystatin F from the secretory pathway and to mediate its uptake from extracellular pools.
Title: Glycosylation directs targeting and activation of cystatin f from intracellular and extracellular sources.

A latent protease inhibitor which is itself regulated by proteolysis in the endocytic pathway.
Title: Cystatin F is a cathepsin C-directed protease inhibitor regulated by proteolysis.

analysis of reduction-dependent activation of human cystatin F
Title: Structural basis of reduction-dependent activation of human cystatin F.

The following CST7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CST7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu55714	NM_003650.4 Latest version!	Homo sapiens cystatin F (CST7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu55714	NM_003650.3	Homo sapiens cystatin F (CST7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CST7 ( NM_003650.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003641.3 Homo sapiens cystatin F (CST7), mRNA.

Price & Availability↑

CloneID	OHu55714
Clone ID Related Accession (Same CDS sequence)	NM_003650.4 , NM_003650.3
Accession Version	NM_003650.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	cystatin-F precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG547285.1, AF031824.1 and CA449286.1. On Nov 23, 2018 this sequence version replaced NM_003650.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM917046.1, SRR5189655.149795.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:9733783) MANE Ensembl match :: ENST00000480798.2/ ENSP00000420384.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCGAGCGG CTGGAACTCT GCTGGCCTTC TGCTGCCTGG TCTTGAGCAC CACTGGGGGC 
CCTTCCCCAG ATACTTGTTC CCAGGACCTT AACTCACGTG TGAAGCCAGG ATTTCCTAAA 
ACAATAAAGA CCAATGACCC AGGAGTCCTC CAAGCAGCCA GATACAGTGT TGAAAAGTTC 
AACAACTGCA CGAACGACAT GTTCTTGTTC AAGGAGTCCC GCATCACAAG GGCCCTAGTT 
CAGATAGTGA AAGGCCTGAA ATATATGCTG GAGGTGGAAA TTGGCAGAAC TACCTGCAAG 
AAAAACCAGC ACCTGCGTCT GGATGACTGT GACTTCCAAA CCAACCACAC CTTGAAGCAG 
ACTCTGAGCT GCTACTCTGA AGTCTGGGTC GTGCCCTGGC TCCAGCACTT CGAGGTGCCT 
GTTCTCCGTT GTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003641.3
CDS	238..675
Translation	MRAAGTLLAFCCLVLSTTGGPSPDTCSQDLNSRVKPGFPKTIKTNDPGVLQAARYSVEKFNNCTNDMFLFKESRITRALVQIVKGLKYMLEVEIGRTTCKKNQHLRLDDCDFQTNHTLKQTLSCYSEVWVVPWLQHFEVPVLRCH

Target ORF information:

RefSeq Version	NM_003650.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cystatin F (CST7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003650.4

ORF Insert Sequence:

ATGCGAGCGG CTGGAACTCT GCTGGCCTTC TGCTGCCTGG TCTTGAGCAC CACTGGGGGC 
CCTTCCCCAG ATACTTGTTC CCAGGACCTT AACTCACGTG TGAAGCCAGG ATTTCCTAAA 
ACAATAAAGA CCAATGACCC AGGAGTCCTC CAAGCAGCCA GATACAGTGT TGAAAAGTTC 
AACAACTGCA CGAACGACAT GTTCTTGTTC AAGGAGTCCC GCATCACAAG GGCCCTAGTT 
CAGATAGTGA AAGGCCTGAA ATATATGCTG GAGGTGGAAA TTGGCAGAAC TACCTGCAAG 
AAAAACCAGC ACCTGCGTCT GGATGACTGT GACTTCCAAA CCAACCACAC CTTGAAGCAG 
ACTCTGAGCT GCTACTCTGA AGTCTGGGTC GTGCCCTGGC TCCAGCACTT CGAGGTGCCT 
GTTCTCCGTT GTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CST7 ( NM_003650.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003641.3 Homo sapiens cystatin F (CST7), mRNA.

Price & Availability↑

CloneID	OHu55714
Clone ID Related Accession (Same CDS sequence)	NM_003650.4 , NM_003650.3
Accession Version	NM_003650.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	cystatin-F precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG547285.1, AF031824.1 and CA449286.1. On Oct 31, 2009 this sequence version replaced NM_003650.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM917046.1, AL559379.3 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:9733783) ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGAGCGG CTGGAACTCT GCTGGCCTTC TGCTGCCTGG TCTTGAGCAC CACTGGGGGC 
CCTTCCCCAG ATACTTGTTC CCAGGACCTT AACTCACGTG TGAAGCCAGG ATTTCCTAAA 
ACAATAAAGA CCAATGACCC AGGAGTCCTC CAAGCAGCCA GATACAGTGT TGAAAAGTTC 
AACAACTGCA CGAACGACAT GTTCTTGTTC AAGGAGTCCC GCATCACAAG GGCCCTAGTT 
CAGATAGTGA AAGGCCTGAA ATATATGCTG GAGGTGGAAA TTGGCAGAAC TACCTGCAAG 
AAAAACCAGC ACCTGCGTCT GGATGACTGT GACTTCCAAA CCAACCACAC CTTGAAGCAG 
ACTCTGAGCT GCTACTCTGA AGTCTGGGTC GTGCCCTGGC TCCAGCACTT CGAGGTGCCT 
GTTCTCCGTT GTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003641.3
CDS	277..714
Translation	MRAAGTLLAFCCLVLSTTGGPSPDTCSQDLNSRVKPGFPKTIKTNDPGVLQAARYSVEKFNNCTNDMFLFKESRITRALVQIVKGLKYMLEVEIGRTTCKKNQHLRLDDCDFQTNHTLKQTLSCYSEVWVVPWLQHFEVPVLRCH

Target ORF information:

RefSeq Version	NM_003650.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cystatin F (CST7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003650.3

ORF Insert Sequence:

ATGCGAGCGG CTGGAACTCT GCTGGCCTTC TGCTGCCTGG TCTTGAGCAC CACTGGGGGC 
CCTTCCCCAG ATACTTGTTC CCAGGACCTT AACTCACGTG TGAAGCCAGG ATTTCCTAAA 
ACAATAAAGA CCAATGACCC AGGAGTCCTC CAAGCAGCCA GATACAGTGT TGAAAAGTTC 
AACAACTGCA CGAACGACAT GTTCTTGTTC AAGGAGTCCC GCATCACAAG GGCCCTAGTT 
CAGATAGTGA AAGGCCTGAA ATATATGCTG GAGGTGGAAA TTGGCAGAAC TACCTGCAAG 
AAAAACCAGC ACCTGCGTCT GGATGACTGT GACTTCCAAA CCAACCACAC CTTGAAGCAG 
ACTCTGAGCT GCTACTCTGA AGTCTGGGTC GTGCCCTGGC TCCAGCACTT CGAGGTGCCT 
GTTCTCCGTT GTCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of cathepsins S and L by cystatin F during maturation of dendritic cells.
European journal of cell biology91(5)391-401(2012 May)
Magister S,Obermajer N,Mirkovi? B,Svajger U,Renko M,Softi? A,Romih R,Colbert JD,Watts C,Kos J

Microglial cystatin F expression is a sensitive indicator for ongoing demyelination with concurrent remyelination.
Journal of neuroscience research89(5)639-49(2011 May)
Ma J,Tanaka KF,Shimizu T,Bernard CC,Kakita A,Takahashi H,Pfeiffer SE,Ikenaka K

Glycosylation directs targeting and activation of cystatin f from intracellular and extracellular sources.
Traffic (Copenhagen, Denmark)10(4)425-37(2009 Apr)
Colbert JD,Plechanovov? A,Watts C

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CST7 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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