RITA1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RITA1
Entrez Gene ID	84934
Full Name	RBPJ interacting and tubulin associated 1
Synonyms	C12orf52,RITA
General protein information	Preferred Names RBPJ interacting and tubulin associated 1 Names RBPJ-interacting and tubulin-associated protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q24.13

mRNA and Protein(s)

mRNA	Protein	Name
XM_005253972.2	XP_005254029.1	RBPJ-interacting and tubulin-associated protein 1 isoform X1
XM_005253972.1	XP_005254029.1	RBPJ-interacting and tubulin-associated protein 1 isoform X1
NM_032848.2	NP_116237.1	RBPJ-interacting and tubulin-associated protein 1 isoform 1
NM_032848.3	NP_116237.1	RBPJ-interacting and tubulin-associated protein 1 isoform 1
XM_005253972.3	XP_005254029.1	RBPJ-interacting and tubulin-associated protein 1 isoform X1
NM_001286215.1	NP_001273144.1	RBPJ-interacting and tubulin-associated protein 1 isoform 2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	RITA1	NP_001039809.1
Homo sapiens (human)	RITA1	NP_001273144.1
Rattus norvegicus (Norway rat)	RGD1306772	NP_001037691.1
Pan troglodytes (chimpanzee)	C12H12orf52	XP_001152471.1
Canis lupus familiaris (dog)	C26H12orf52	XP_005636310.1
Mus musculus (house mouse)	Rita1	NP_083372.1
	C11H12orf52	XP_001111319.2
Xenopus tropicalis (tropical clawed frog)	LOC100491156	XP_002934379.1

Gene Ontology
Bibliography

Related articles in PubMed

Structure-function analysis of RBP-J-interacting and tubulin-associated (RITA) reveals regions critical for repression of Notch target genes.
Tabaja N, Yuan Z, Oswald F, Kovall RA
The Journal of biological chemistry292(25)10549-10563(2017 06)

Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
Li X, Wang W, Wang J, Malovannaya A, Xi Y, Li W, Guerra R, Hawke DH, Qin J, Chen J
Molecular systems biology11(1)775(2015 Jan)

Analysis of the interaction between human RITA and Drosophila Suppressor of Hairless.
Brockmann B, Mastel H, Oswald F, Maier D
Hereditas151(6)209-19(2014 Dec)

RBP-J-interacting and tubulin-associated protein induces apoptosis and cell cycle arrest in human hepatocellular carcinoma by activating the p53-Fbxw7 pathway.
Wang H, Yang Z, Liu C, Huang S, Wang H, Chen Y, Chen G
Biochemical and biophysical research communications454(1)71-7(2014 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

structural and biophysical studies demonstrate that RITA binds RBP-J similarly to the RAM (RBP-J-associated molecule) domain of Notch, our biochemical and cellular assays suggest that RITA interacts with additional regions in RBP-J.
Title: Structure-function analysis of RBP-J-interacting and tubulin-associated (RITA) reveals regions critical for repression of Notch target genes.

human RITA, binding to Drosophila Su(H) and tubulin, cannot influence the Notch signalling pathway in the fly
Title: Analysis of the interaction between human RITA and Drosophila Suppressor of Hairless.

RITA overexpression increased expression of p53 and Fbxw7 and downregulated expression of cyclin D1, cyclin E, CDK2, Hes-1 and NF-kappaB p65. These changes led to growth inhibition and induced G0/G1 cell cycle arrest and apoptosis in SMMC7721 and HepG2 cells.
Title: RBP-J-interacting and tubulin-associated protein induces apoptosis and cell cycle arrest in human hepatocellular carcinoma by activating the p53-Fbxw7 pathway.

RITA might suppress tumor growth and induce apoptosis in hepatocellular carcinoma.
Title: RITA inhibits growth of human hepatocellular carcinoma through induction of apoptosis.

The following RITA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RITA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu09536	NM_032848.3 Latest version!	Homo sapiens RBPJ interacting and tubulin associated 1 (RITA1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu09537	NM_001286215.1 Latest version!	Homo sapiens RBPJ interacting and tubulin associated 1 (RITA1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu09536	XM_005253972.3 Latest version!	Homo sapiens RBPJ interacting and tubulin associated 1 (RITA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu09536	XM_005253972.1	Homo sapiens RBPJ interacting and tubulin associated 1 (RITA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu09536	NM_032848.2	Homo sapiens RBPJ interacting and tubulin associated 1 (RITA1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu09536	XM_005253972.2	Homo sapiens RBPJ interacting and tubulin associated 1 (RITA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RITA1 ( NM_032848.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_116237.1 Homo sapiens RBPJ interacting and tubulin associated 1 (RITA1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09536
Clone ID Related Accession (Same CDS sequence)	XM_005253972.2 , XM_005253972.1 , NM_032848.2 , NM_032848.3 , XM_005253972.3
Accession Version	NM_032848.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	RBPJ-interacting and tubulin-associated protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BP212183.1, DA207985.1, AK027733.1 and AA594422.1. On Oct 31, 2013 this sequence version replaced NM_032848.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the shorter isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1803617.220068.1, SRR1660807.6555.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGACCC CCGTGGAGCT GGCCGTCAGT GGGATGCAGA CCCTCGGCCT TCAGCACCGC 
TGCCGAGGTG GCTACCGGGT CAAGGCCAGG ACGTCATATG TGGATGAGAC TCTGTTTGGC 
AGCCCAGCAG GCACCCGGCC TACCCCACCG GACTTCGATC CGCCCTGGGT GGAGAAGGCT 
AACAGAACCA GAGGCGTGGG CAAGGAGGCA TCGAAGGCCT TGGGGGCAAA GGGGAGCTGT 
GAGACCACCC CCTCAAGGGG CAGCACCCCC ACCCTCACAC CAAGGAAGAA GAACAAATAC 
AGACCCATCA GCCACACCCC GTCTTACTGT GATGAGTCGC TGTTTGGCTC CCGATCTGAA 
GGCGCCAGCT TCGGGGCCCC GCGGATGGCG AAGGGGGATG CCGCAAAGCT CCGTGCTCTC 
TTGTGGACGC CACCACCTAC CCCCAGGGGT AGCCACTCGC CCCGCCCCAG GGAGGCACCA 
CTGCGAGCCA TTCACCCAGC TGGTCCCTCC AAGACAGAGC CGGGGCCAGC GGCAGACTCC 
CAGAAGTTAT CTATGGGTGG GTTACACTCT TCACGCCCCC TGAAGCGGGG ACTTTCCCAT 
TCCCTCACCC ACCTGAATGT CCCCAGCACT GGTCATCCAG CCACCAGTGC CCCCCACACA 
AATGGGCCTC AGGATCTCAG GCCTTCCACG TCAGGGGTGA CCTTCCGGAG CCCCCTGGTG 
ACTTCCAGGG CTCGCTCAGT TAGCATTTCA GTGCCATCTA CCCCACGACG AGGTGGGGCC 
ACCCAGAAAC CAAAGCCCCC TTGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116237.1
CDS	693..1502
Translation	MKTPVELAVSGMQTLGLQHRCRGGYRVKARTSYVDETLFGSPAGTRPTPPDFDPPWVEKANRTRGVGKEASKALGAKGSCETTPSRGSTPTLTPRKKNKYRPISHTPSYCDESLFGSRSEGASFGAPRMAKGDAAKLRALLWTPPPTPRGSHSPRPREAPLRAIHPAGPSKTEPGPAADSQKLSMGGLHSSRPLKRGLSHSLTHLNVPSTGHPATSAPHTNGPQDLRPSTSGVTFRSPLVTSRARSVSISVPSTPRRGGATQKPKPPWK

Target ORF information:

RefSeq Version	NM_032848.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RBPJ interacting and tubulin associated 1 (RITA1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032848.2

ORF Insert Sequence:

ATGAAGACCC CCGTGGAGCT GGCCGTCAGT GGGATGCAGA CCCTCGGCCT TCAGCACCGC 
TGCCGAGGTG GCTACCGGGT CAAGGCCAGG ACGTCATATG TGGATGAGAC TCTGTTTGGC 
AGCCCAGCAG GCACCCGGCC TACCCCACCG GACTTCGATC CGCCCTGGGT GGAGAAGGCT 
AACAGAACCA GAGGCGTGGG CAAGGAGGCA TCGAAGGCCT TGGGGGCAAA GGGGAGCTGT 
GAGACCACCC CCTCAAGGGG CAGCACCCCC ACCCTCACAC CAAGGAAGAA GAACAAATAC 
AGACCCATCA GCCACACCCC GTCTTACTGT GATGAGTCGC TGTTTGGCTC CCGATCTGAA 
GGCGCCAGCT TCGGGGCCCC GCGGATGGCG AAGGGGGATG CCGCAAAGCT CCGTGCTCTC 
TTGTGGACGC CACCACCTAC CCCCAGGGGT AGCCACTCGC CCCGCCCCAG GGAGGCACCA 
CTGCGAGCCA TTCACCCAGC TGGTCCCTCC AAGACAGAGC CGGGGCCAGC GGCAGACTCC 
CAGAAGTTAT CTATGGGTGG GTTACACTCT TCACGCCCCC TGAAGCGGGG ACTTTCCCAT 
TCCCTCACCC ACCTGAATGT CCCCAGCACT GGTCATCCAG CCACCAGTGC CCCCCACACA 
AATGGGCCTC AGGATCTCAG GCCTTCCACG TCAGGGGTGA CCTTCCGGAG CCCCCTGGTG 
ACTTCCAGGG CTCGCTCAGT TAGCATTTCA GTGCCATCTA CCCCACGACG AGGTGGGGCC 
ACCCAGAAAC CAAAGCCCCC TTGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RITA1 ( NM_032848.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_116237.1 Homo sapiens RBPJ interacting and tubulin associated 1 (RITA1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu09536
Clone ID Related Accession (Same CDS sequence)	XM_005253972.2 , XM_005253972.1 , NM_032848.2 , NM_032848.3 , XM_005253972.3
Accession Version	NM_032848.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	RBPJ-interacting and tubulin-associated protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA207985.1, AK027733.1 and AA594422.1. On Nov 23, 2018 this sequence version replaced NM_032848.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the shorter isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR3476690.684349.1, SRR3476690.1160026.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000548278.2/ ENSP00000449841.1 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAAGACCC CCGTGGAGCT GGCCGTCAGT GGGATGCAGA CCCTCGGCCT TCAGCACCGC 
TGCCGAGGTG GCTACCGGGT CAAGGCCAGG ACGTCATATG TGGATGAGAC TCTGTTTGGC 
AGCCCAGCAG GCACCCGGCC TACCCCACCG GACTTCGATC CGCCCTGGGT GGAGAAGGCT 
AACAGAACCA GAGGCGTGGG CAAGGAGGCA TCGAAGGCCT TGGGGGCAAA GGGGAGCTGT 
GAGACCACCC CCTCAAGGGG CAGCACCCCC ACCCTCACAC CAAGGAAGAA GAACAAATAC 
AGACCCATCA GCCACACCCC GTCTTACTGT GATGAGTCGC TGTTTGGCTC CCGATCTGAA 
GGCGCCAGCT TCGGGGCCCC GCGGATGGCG AAGGGGGATG CCGCAAAGCT CCGTGCTCTC 
TTGTGGACGC CACCACCTAC CCCCAGGGGT AGCCACTCGC CCCGCCCCAG GGAGGCACCA 
CTGCGAGCCA TTCACCCAGC TGGTCCCTCC AAGACAGAGC CGGGGCCAGC GGCAGACTCC 
CAGAAGTTAT CTATGGGTGG GTTACACTCT TCACGCCCCC TGAAGCGGGG ACTTTCCCAT 
TCCCTCACCC ACCTGAATGT CCCCAGCACT GGTCATCCAG CCACCAGTGC CCCCCACACA 
AATGGGCCTC AGGATCTCAG GCCTTCCACG TCAGGGGTGA CCTTCCGGAG CCCCCTGGTG 
ACTTCCAGGG CTCGCTCAGT TAGCATTTCA GTGCCATCTA CCCCACGACG AGGTGGGGCC 
ACCCAGAAAC CAAAGCCCCC TTGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116237.1
CDS	498..1307
Translation	MKTPVELAVSGMQTLGLQHRCRGGYRVKARTSYVDETLFGSPAGTRPTPPDFDPPWVEKANRTRGVGKEASKALGAKGSCETTPSRGSTPTLTPRKKNKYRPISHTPSYCDESLFGSRSEGASFGAPRMAKGDAAKLRALLWTPPPTPRGSHSPRPREAPLRAIHPAGPSKTEPGPAADSQKLSMGGLHSSRPLKRGLSHSLTHLNVPSTGHPATSAPHTNGPQDLRPSTSGVTFRSPLVTSRARSVSISVPSTPRRGGATQKPKPPWK

Target ORF information:

RefSeq Version	NM_032848.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RBPJ interacting and tubulin associated 1 (RITA1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032848.3

ORF Insert Sequence:

ATGAAGACCC CCGTGGAGCT GGCCGTCAGT GGGATGCAGA CCCTCGGCCT TCAGCACCGC 
TGCCGAGGTG GCTACCGGGT CAAGGCCAGG ACGTCATATG TGGATGAGAC TCTGTTTGGC 
AGCCCAGCAG GCACCCGGCC TACCCCACCG GACTTCGATC CGCCCTGGGT GGAGAAGGCT 
AACAGAACCA GAGGCGTGGG CAAGGAGGCA TCGAAGGCCT TGGGGGCAAA GGGGAGCTGT 
GAGACCACCC CCTCAAGGGG CAGCACCCCC ACCCTCACAC CAAGGAAGAA GAACAAATAC 
AGACCCATCA GCCACACCCC GTCTTACTGT GATGAGTCGC TGTTTGGCTC CCGATCTGAA 
GGCGCCAGCT TCGGGGCCCC GCGGATGGCG AAGGGGGATG CCGCAAAGCT CCGTGCTCTC 
TTGTGGACGC CACCACCTAC CCCCAGGGGT AGCCACTCGC CCCGCCCCAG GGAGGCACCA 
CTGCGAGCCA TTCACCCAGC TGGTCCCTCC AAGACAGAGC CGGGGCCAGC GGCAGACTCC 
CAGAAGTTAT CTATGGGTGG GTTACACTCT TCACGCCCCC TGAAGCGGGG ACTTTCCCAT 
TCCCTCACCC ACCTGAATGT CCCCAGCACT GGTCATCCAG CCACCAGTGC CCCCCACACA 
AATGGGCCTC AGGATCTCAG GCCTTCCACG TCAGGGGTGA CCTTCCGGAG CCCCCTGGTG 
ACTTCCAGGG CTCGCTCAGT TAGCATTTCA GTGCCATCTA CCCCACGACG AGGTGGGGCC 
ACCCAGAAAC CAAAGCCCCC TTGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RITA1 ( NM_001286215.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273144.1 Homo sapiens RBPJ interacting and tubulin associated 1 (RITA1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu09537
Clone ID Related Accession (Same CDS sequence)	NM_001286215.1
Accession Version	NM_001286215.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	RBPJ-interacting and tubulin-associated protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BP212183.1, DA207985.1, BX366456.2, AK027733.1 and AA594422.1. On Oct 31, 2013 this sequence version replaced XM_005253971.1. Transcript Variant: This variant (2) lacks an alternate exon in the 5' region and initiates translation at an alternate upstream start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus and is longer than isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.219640.1, BX366455.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTCAGGG AGCCTCGGAA GCAGGGCCTG GCCGGCAGAG CACACCTGCT GTCACCAGGG 
ACCACAGGCA GCATGAAGAC CCCCGTGGAG CTGGCCGTCA GTGGGATGCA GACCCTCGGC 
CTTCAGCACC GCTGCCGAGG TGGCTACCGG GTCAAGGCCA GGACGTCATA TGTGGATGAG 
ACTCTGTTTG GCAGCCCAGC AGGCACCCGG CCTACCCCAC CGGACTTCGA TCCGCCCTGG 
GTGGAGAAGG CTAACAGAAC CAGAGGCGTG GGCAAGGAGG CATCGAAGGC CTTGGGGGCA 
AAGGGGAGCT GTGAGACCAC CCCCTCAAGG GGCAGCACCC CCACCCTCAC ACCAAGGAAG 
AAGAACAAAT ACAGACCCAT CAGCCACACC CCGTCTTACT GTGATGAGTC GCTGTTTGGC 
TCCCGATCTG AAGGCGCCAG CTTCGGGGCC CCGCGGATGG CGAAGGGGGA TGCCGCAAAG 
CTCCGTGCTC TCTTGTGGAC GCCACCACCT ACCCCCAGGG GTAGCCACTC GCCCCGCCCC 
AGGGAGGCAC CACTGCGAGC CATTCACCCA GCTGGTCCCT CCAAGACAGA GCCGGGGCCA 
GCGGCAGACT CCCAGAAGTT ATCTATGGGT GGGTTACACT CTTCACGCCC CCTGAAGCGG 
GGACTTTCCC ATTCCCTCAC CCACCTGAAT GTCCCCAGCA CTGGTCATCC AGCCACCAGT 
GCCCCCCACA CAAATGGGCC TCAGGATCTC AGGCCTTCCA CGTCAGGGGT GACCTTCCGG 
AGCCCCCTGG TGACTTCCAG GGCTCGCTCA GTTAGCATTT CAGTGCCATC TACCCCACGA 
CGAGGTGGGG CCACCCAGAA ACCAAAGCCC CCTTGGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273144.1
CDS	489..1370
Translation	MLREPRKQGLAGRAHLLSPGTTGSMKTPVELAVSGMQTLGLQHRCRGGYRVKARTSYVDETLFGSPAGTRPTPPDFDPPWVEKANRTRGVGKEASKALGAKGSCETTPSRGSTPTLTPRKKNKYRPISHTPSYCDESLFGSRSEGASFGAPRMAKGDAAKLRALLWTPPPTPRGSHSPRPREAPLRAIHPAGPSKTEPGPAADSQKLSMGGLHSSRPLKRGLSHSLTHLNVPSTGHPATSAPHTNGPQDLRPSTSGVTFRSPLVTSRARSVSISVPSTPRRGGATQKPKPPWK

Target ORF information:

RefSeq Version	NM_001286215.1
Organism	Homo sapiens(human)
Definition	Homo sapiens RBPJ interacting and tubulin associated 1 (RITA1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286215.1

ORF Insert Sequence:

ATGCTCAGGG AGCCTCGGAA GCAGGGCCTG GCCGGCAGAG CACACCTGCT GTCACCAGGG 
ACCACAGGCA GCATGAAGAC CCCCGTGGAG CTGGCCGTCA GTGGGATGCA GACCCTCGGC 
CTTCAGCACC GCTGCCGAGG TGGCTACCGG GTCAAGGCCA GGACGTCATA TGTGGATGAG 
ACTCTGTTTG GCAGCCCAGC AGGCACCCGG CCTACCCCAC CGGACTTCGA TCCGCCCTGG 
GTGGAGAAGG CTAACAGAAC CAGAGGCGTG GGCAAGGAGG CATCGAAGGC CTTGGGGGCA 
AAGGGGAGCT GTGAGACCAC CCCCTCAAGG GGCAGCACCC CCACCCTCAC ACCAAGGAAG 
AAGAACAAAT ACAGACCCAT CAGCCACACC CCGTCTTACT GTGATGAGTC GCTGTTTGGC 
TCCCGATCTG AAGGCGCCAG CTTCGGGGCC CCGCGGATGG CGAAGGGGGA TGCCGCAAAG 
CTCCGTGCTC TCTTGTGGAC GCCACCACCT ACCCCCAGGG GTAGCCACTC GCCCCGCCCC 
AGGGAGGCAC CACTGCGAGC CATTCACCCA GCTGGTCCCT CCAAGACAGA GCCGGGGCCA 
GCGGCAGACT CCCAGAAGTT ATCTATGGGT GGGTTACACT CTTCACGCCC CCTGAAGCGG 
GGACTTTCCC ATTCCCTCAC CCACCTGAAT GTCCCCAGCA CTGGTCATCC AGCCACCAGT 
GCCCCCCACA CAAATGGGCC TCAGGATCTC AGGCCTTCCA CGTCAGGGGT GACCTTCCGG 
AGCCCCCTGG TGACTTCCAG GGCTCGCTCA GTTAGCATTT CAGTGCCATC TACCCCACGA 
CGAGGTGGGG CCACCCAGAA ACCAAAGCCC CCTTGGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RITA1 ( XM_005253972.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005254029.1 Homo sapiens RBPJ interacting and tubulin associated 1 (RITA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu09536
Clone ID Related Accession (Same CDS sequence)	XM_005253972.2 , XM_005253972.1 , NM_032848.2 , NM_032848.3 , XM_005253972.3
Accession Version	XM_005253972.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	RBPJ-interacting and tubulin-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005253972.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACCC CCGTGGAGCT GGCCGTCAGT GGGATGCAGA CCCTCGGCCT TCAGCACCGC 
TGCCGAGGTG GCTACCGGGT CAAGGCCAGG ACGTCATATG TGGATGAGAC TCTGTTTGGC 
AGCCCAGCAG GCACCCGGCC TACCCCACCG GACTTCGATC CGCCCTGGGT GGAGAAGGCT 
AACAGAACCA GAGGCGTGGG CAAGGAGGCA TCGAAGGCCT TGGGGGCAAA GGGGAGCTGT 
GAGACCACCC CCTCAAGGGG CAGCACCCCC ACCCTCACAC CAAGGAAGAA GAACAAATAC 
AGACCCATCA GCCACACCCC GTCTTACTGT GATGAGTCGC TGTTTGGCTC CCGATCTGAA 
GGCGCCAGCT TCGGGGCCCC GCGGATGGCG AAGGGGGATG CCGCAAAGCT CCGTGCTCTC 
TTGTGGACGC CACCACCTAC CCCCAGGGGT AGCCACTCGC CCCGCCCCAG GGAGGCACCA 
CTGCGAGCCA TTCACCCAGC TGGTCCCTCC AAGACAGAGC CGGGGCCAGC GGCAGACTCC 
CAGAAGTTAT CTATGGGTGG GTTACACTCT TCACGCCCCC TGAAGCGGGG ACTTTCCCAT 
TCCCTCACCC ACCTGAATGT CCCCAGCACT GGTCATCCAG CCACCAGTGC CCCCCACACA 
AATGGGCCTC AGGATCTCAG GCCTTCCACG TCAGGGGTGA CCTTCCGGAG CCCCCTGGTG 
ACTTCCAGGG CTCGCTCAGT TAGCATTTCA GTGCCATCTA CCCCACGACG AGGTGGGGCC 
ACCCAGAAAC CAAAGCCCCC TTGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005254029.1
CDS	665..1474
Translation	MKTPVELAVSGMQTLGLQHRCRGGYRVKARTSYVDETLFGSPAGTRPTPPDFDPPWVEKANRTRGVGKEASKALGAKGSCETTPSRGSTPTLTPRKKNKYRPISHTPSYCDESLFGSRSEGASFGAPRMAKGDAAKLRALLWTPPPTPRGSHSPRPREAPLRAIHPAGPSKTEPGPAADSQKLSMGGLHSSRPLKRGLSHSLTHLNVPSTGHPATSAPHTNGPQDLRPSTSGVTFRSPLVTSRARSVSISVPSTPRRGGATQKPKPPWK

Target ORF information:

RefSeq Version	XM_005253972.2
Organism	Homo sapiens(human)
Definition	Homo sapiens RBPJ interacting and tubulin associated 1 (RITA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005253972.2

ORF Insert Sequence:

ATGAAGACCC CCGTGGAGCT GGCCGTCAGT GGGATGCAGA CCCTCGGCCT TCAGCACCGC 
TGCCGAGGTG GCTACCGGGT CAAGGCCAGG ACGTCATATG TGGATGAGAC TCTGTTTGGC 
AGCCCAGCAG GCACCCGGCC TACCCCACCG GACTTCGATC CGCCCTGGGT GGAGAAGGCT 
AACAGAACCA GAGGCGTGGG CAAGGAGGCA TCGAAGGCCT TGGGGGCAAA GGGGAGCTGT 
GAGACCACCC CCTCAAGGGG CAGCACCCCC ACCCTCACAC CAAGGAAGAA GAACAAATAC 
AGACCCATCA GCCACACCCC GTCTTACTGT GATGAGTCGC TGTTTGGCTC CCGATCTGAA 
GGCGCCAGCT TCGGGGCCCC GCGGATGGCG AAGGGGGATG CCGCAAAGCT CCGTGCTCTC 
TTGTGGACGC CACCACCTAC CCCCAGGGGT AGCCACTCGC CCCGCCCCAG GGAGGCACCA 
CTGCGAGCCA TTCACCCAGC TGGTCCCTCC AAGACAGAGC CGGGGCCAGC GGCAGACTCC 
CAGAAGTTAT CTATGGGTGG GTTACACTCT TCACGCCCCC TGAAGCGGGG ACTTTCCCAT 
TCCCTCACCC ACCTGAATGT CCCCAGCACT GGTCATCCAG CCACCAGTGC CCCCCACACA 
AATGGGCCTC AGGATCTCAG GCCTTCCACG TCAGGGGTGA CCTTCCGGAG CCCCCTGGTG 
ACTTCCAGGG CTCGCTCAGT TAGCATTTCA GTGCCATCTA CCCCACGACG AGGTGGGGCC 
ACCCAGAAAC CAAAGCCCCC TTGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RITA1 ( XM_005253972.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005254029.1 Homo sapiens RBPJ interacting and tubulin associated 1 (RITA1), transcript variant X1, mRNA.

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CloneID	OHu09536
Clone ID Related Accession (Same CDS sequence)	XM_005253972.2 , XM_005253972.1 , NM_032848.2 , NM_032848.3 , XM_005253972.3
Accession Version	XM_005253972.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	RBPJ-interacting and tubulin-associated protein 1 isoform X1
Comment

ATGAAGACCC CCGTGGAGCT GGCCGTCAGT GGGATGCAGA CCCTCGGCCT TCAGCACCGC 
TGCCGAGGTG GCTACCGGGT CAAGGCCAGG ACGTCATATG TGGATGAGAC TCTGTTTGGC 
AGCCCAGCAG GCACCCGGCC TACCCCACCG GACTTCGATC CGCCCTGGGT GGAGAAGGCT 
AACAGAACCA GAGGCGTGGG CAAGGAGGCA TCGAAGGCCT TGGGGGCAAA GGGGAGCTGT 
GAGACCACCC CCTCAAGGGG CAGCACCCCC ACCCTCACAC CAAGGAAGAA GAACAAATAC 
AGACCCATCA GCCACACCCC GTCTTACTGT GATGAGTCGC TGTTTGGCTC CCGATCTGAA 
GGCGCCAGCT TCGGGGCCCC GCGGATGGCG AAGGGGGATG CCGCAAAGCT CCGTGCTCTC 
TTGTGGACGC CACCACCTAC CCCCAGGGGT AGCCACTCGC CCCGCCCCAG GGAGGCACCA 
CTGCGAGCCA TTCACCCAGC TGGTCCCTCC AAGACAGAGC CGGGGCCAGC GGCAGACTCC 
CAGAAGTTAT CTATGGGTGG GTTACACTCT TCACGCCCCC TGAAGCGGGG ACTTTCCCAT 
TCCCTCACCC ACCTGAATGT CCCCAGCACT GGTCATCCAG CCACCAGTGC CCCCCACACA 
AATGGGCCTC AGGATCTCAG GCCTTCCACG TCAGGGGTGA CCTTCCGGAG CCCCCTGGTG 
ACTTCCAGGG CTCGCTCAGT TAGCATTTCA GTGCCATCTA CCCCACGACG AGGTGGGGCC 
ACCCAGAAAC CAAAGCCCCC TTGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005254029.1
Translation	MKTPVELAVSGMQTLGLQHRCRGGYRVKARTSYVDETLFGSPAGTRPTPPDFDPPWVEKANRTRGVGKEASKALGAKGSCETTPSRGSTPTLTPRKKNKYRPISHTPSYCDESLFGSRSEGASFGAPRMAKGDAAKLRALLWTPPPTPRGSHSPRPREAPLRAIHPAGPSKTEPGPAADSQKLSMGGLHSSRPLKRGLSHSLTHLNVPSTGHPATSAPHTNGPQDLRPSTSGVTFRSPLVTSRARSVSISVPSTPRRGGATQKPKPPWK

Target ORF information:

RefSeq Version	XM_005253972.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens RBPJ interacting and tubulin associated 1 (RITA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005253972.1

ORF Insert Sequence:

ATGAAGACCC CCGTGGAGCT GGCCGTCAGT GGGATGCAGA CCCTCGGCCT TCAGCACCGC 
TGCCGAGGTG GCTACCGGGT CAAGGCCAGG ACGTCATATG TGGATGAGAC TCTGTTTGGC 
AGCCCAGCAG GCACCCGGCC TACCCCACCG GACTTCGATC CGCCCTGGGT GGAGAAGGCT 
AACAGAACCA GAGGCGTGGG CAAGGAGGCA TCGAAGGCCT TGGGGGCAAA GGGGAGCTGT 
GAGACCACCC CCTCAAGGGG CAGCACCCCC ACCCTCACAC CAAGGAAGAA GAACAAATAC 
AGACCCATCA GCCACACCCC GTCTTACTGT GATGAGTCGC TGTTTGGCTC CCGATCTGAA 
GGCGCCAGCT TCGGGGCCCC GCGGATGGCG AAGGGGGATG CCGCAAAGCT CCGTGCTCTC 
TTGTGGACGC CACCACCTAC CCCCAGGGGT AGCCACTCGC CCCGCCCCAG GGAGGCACCA 
CTGCGAGCCA TTCACCCAGC TGGTCCCTCC AAGACAGAGC CGGGGCCAGC GGCAGACTCC 
CAGAAGTTAT CTATGGGTGG GTTACACTCT TCACGCCCCC TGAAGCGGGG ACTTTCCCAT 
TCCCTCACCC ACCTGAATGT CCCCAGCACT GGTCATCCAG CCACCAGTGC CCCCCACACA 
AATGGGCCTC AGGATCTCAG GCCTTCCACG TCAGGGGTGA CCTTCCGGAG CCCCCTGGTG 
ACTTCCAGGG CTCGCTCAGT TAGCATTTCA GTGCCATCTA CCCCACGACG AGGTGGGGCC 
ACCCAGAAAC CAAAGCCCCC TTGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RITA1 ( XM_005253972.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005254029.1 Homo sapiens RBPJ interacting and tubulin associated 1 (RITA1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu09536
Clone ID Related Accession (Same CDS sequence)	XM_005253972.2 , XM_005253972.1 , NM_032848.2 , NM_032848.3 , XM_005253972.3
Accession Version	XM_005253972.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	RBPJ-interacting and tubulin-associated protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005253972.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGACCC CCGTGGAGCT GGCCGTCAGT GGGATGCAGA CCCTCGGCCT TCAGCACCGC 
TGCCGAGGTG GCTACCGGGT CAAGGCCAGG ACGTCATATG TGGATGAGAC TCTGTTTGGC 
AGCCCAGCAG GCACCCGGCC TACCCCACCG GACTTCGATC CGCCCTGGGT GGAGAAGGCT 
AACAGAACCA GAGGCGTGGG CAAGGAGGCA TCGAAGGCCT TGGGGGCAAA GGGGAGCTGT 
GAGACCACCC CCTCAAGGGG CAGCACCCCC ACCCTCACAC CAAGGAAGAA GAACAAATAC 
AGACCCATCA GCCACACCCC GTCTTACTGT GATGAGTCGC TGTTTGGCTC CCGATCTGAA 
GGCGCCAGCT TCGGGGCCCC GCGGATGGCG AAGGGGGATG CCGCAAAGCT CCGTGCTCTC 
TTGTGGACGC CACCACCTAC CCCCAGGGGT AGCCACTCGC CCCGCCCCAG GGAGGCACCA 
CTGCGAGCCA TTCACCCAGC TGGTCCCTCC AAGACAGAGC CGGGGCCAGC GGCAGACTCC 
CAGAAGTTAT CTATGGGTGG GTTACACTCT TCACGCCCCC TGAAGCGGGG ACTTTCCCAT 
TCCCTCACCC ACCTGAATGT CCCCAGCACT GGTCATCCAG CCACCAGTGC CCCCCACACA 
AATGGGCCTC AGGATCTCAG GCCTTCCACG TCAGGGGTGA CCTTCCGGAG CCCCCTGGTG 
ACTTCCAGGG CTCGCTCAGT TAGCATTTCA GTGCCATCTA CCCCACGACG AGGTGGGGCC 
ACCCAGAAAC CAAAGCCCCC TTGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005254029.1
CDS	651..1460
Translation	MKTPVELAVSGMQTLGLQHRCRGGYRVKARTSYVDETLFGSPAGTRPTPPDFDPPWVEKANRTRGVGKEASKALGAKGSCETTPSRGSTPTLTPRKKNKYRPISHTPSYCDESLFGSRSEGASFGAPRMAKGDAAKLRALLWTPPPTPRGSHSPRPREAPLRAIHPAGPSKTEPGPAADSQKLSMGGLHSSRPLKRGLSHSLTHLNVPSTGHPATSAPHTNGPQDLRPSTSGVTFRSPLVTSRARSVSISVPSTPRRGGATQKPKPPWK

Target ORF information:

RefSeq Version	XM_005253972.3
Organism	Homo sapiens(human)
Definition	Homo sapiens RBPJ interacting and tubulin associated 1 (RITA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005253972.3

ORF Insert Sequence:

ATGAAGACCC CCGTGGAGCT GGCCGTCAGT GGGATGCAGA CCCTCGGCCT TCAGCACCGC 
TGCCGAGGTG GCTACCGGGT CAAGGCCAGG ACGTCATATG TGGATGAGAC TCTGTTTGGC 
AGCCCAGCAG GCACCCGGCC TACCCCACCG GACTTCGATC CGCCCTGGGT GGAGAAGGCT 
AACAGAACCA GAGGCGTGGG CAAGGAGGCA TCGAAGGCCT TGGGGGCAAA GGGGAGCTGT 
GAGACCACCC CCTCAAGGGG CAGCACCCCC ACCCTCACAC CAAGGAAGAA GAACAAATAC 
AGACCCATCA GCCACACCCC GTCTTACTGT GATGAGTCGC TGTTTGGCTC CCGATCTGAA 
GGCGCCAGCT TCGGGGCCCC GCGGATGGCG AAGGGGGATG CCGCAAAGCT CCGTGCTCTC 
TTGTGGACGC CACCACCTAC CCCCAGGGGT AGCCACTCGC CCCGCCCCAG GGAGGCACCA 
CTGCGAGCCA TTCACCCAGC TGGTCCCTCC AAGACAGAGC CGGGGCCAGC GGCAGACTCC 
CAGAAGTTAT CTATGGGTGG GTTACACTCT TCACGCCCCC TGAAGCGGGG ACTTTCCCAT 
TCCCTCACCC ACCTGAATGT CCCCAGCACT GGTCATCCAG CCACCAGTGC CCCCCACACA 
AATGGGCCTC AGGATCTCAG GCCTTCCACG TCAGGGGTGA CCTTCCGGAG CCCCCTGGTG 
ACTTCCAGGG CTCGCTCAGT TAGCATTTCA GTGCCATCTA CCCCACGACG AGGTGGGGCC 
ACCCAGAAAC CAAAGCCCCC TTGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structure-function analysis of RBP-J-interacting and tubulin-associated (RITA) reveals regions critical for repression of Notch target genes.
The Journal of biological chemistry292(25)10549-10563(2017 06)
Tabaja N,Yuan Z,Oswald F,Kovall RA

Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes.
Molecular systems biology11(1)775(2015 Jan)
Li X,Wang W,Wang J,Malovannaya A,Xi Y,Li W,Guerra R,Hawke DH,Qin J,Chen J

Analysis of the interaction between human RITA and Drosophila Suppressor of Hairless.
Hereditas151(6)209-19(2014 Dec)
Brockmann B,Mastel H,Oswald F,Maier D

RBP-J-interacting and tubulin-associated protein induces apoptosis and cell cycle arrest in human hepatocellular carcinoma by activating the p53-Fbxw7 pathway.
Biochemical and biophysical research communications454(1)71-7(2014 Nov)
Wang H,Yang Z,Liu C,Huang S,Wang H,Chen Y,Chen G

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RITA1 cDNA ORF clone, Homo sapiens(human)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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