PPP1R15B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PPP1R15B
Entrez Gene ID	84919
Full Name	protein phosphatase 1 regulatory subunit 15B
Synonyms	CREP,MSSGM2
General protein information	Preferred Names protein phosphatase 1 regulatory subunit 15B Names protein phosphatase 1 regulatory subunit 15B protein phosphatase 1, regulatory (inhibitor) subunit 15B
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q32.1
Summary	This gene encodes a protein phosphatase I-interacting protein that promotes the dephosphorylation of eukaryotic translation initiation factor 2A to regulate translation under conditions of cellular stress. The transcribed messenger RNA contains two upstream open reading frames (ORFs) that repress translation of the main protein coding ORF under normal conditions, while the protein coding ORF is expressed at high levels in response to stress. Continual translation of the mRNA under conditions of eukaryotic translation initiation factor 2A inactivation is thought to create a feedback loop for reactivation of the gene during recovery from stress. In addition, it has been shown that this protein plays a role in membrane traffic that is independent of translation and that it is required for exocytosis from erythroleukemia cells. Allelic variants of this gene are associated with microcephaly, short stature, and impaired glucose metabolism. [provided by RefSeq, Feb 2016].
Disorder MIM:	613257
Disorder Html:	Microcephaly, short stature, and impaired glucose metabolism 2, 616817 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005245551.5	XP_005245608.2	protein phosphatase 1 regulatory subunit 15B isoform X1
NM_032833.4	NP_116222.4	protein phosphatase 1 regulatory subunit 15B
XM_005245551.4	XP_005245608.2	protein phosphatase 1 regulatory subunit 15B isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PPP1R15B	XP_545680.3
Bos taurus (cattle)	PPP1R15B	XP_002693950.1
Gallus gallus (chicken)	PPP1R15B	XP_003642789.2
Homo sapiens (human)	PPP1R15B	NP_116222.3
	PPP1R15B	XP_001097695.1
Pan troglodytes (chimpanzee)	PPP1R15B	XP_001159260.1
Mus musculus (house mouse)	Ppp1r15b	NP_598580.1
Rattus norvegicus (Norway rat)	Ppp1r15b	NP_001100645.1

Gene Ontology
Bibliography

Related articles in PubMed

Systematic Analysis of Human Protein Phosphatase Interactions and Dynamics.
Yadav L, Tamene F, G??s H, van Drogen A, Katainen R, Aebersold R, Gstaiger M, Varjosalo M
Cell systems4(4)430-444.e5(2017 Apr)

A Missense Mutation in PPP1R15B Causes a Syndrome Including Diabetes, Short Stature, and Microcephaly.
Abdulkarim B, Nicolino M, Igoillo-Esteve M, Daures M, Romero S, Philippi A, Sen?e V, Lopes M, Cunha DA, Harding HP, Derbois C, Bendelac N, Hattersley AT, Eizirik DL, Ron D, Cnop M, Julier C
Diabetes64(11)3951-62(2015 Nov)

Homozygous mutation in the eukaryotic translation initiation factor 2alpha phosphatase gene, PPP1R15B, is associated with severe microcephaly, short stature and intellectual disability.
Kernohan KD, T?treault M, Liwak-Muir U, Geraghty MT, Qin W, Venkateswaran S, Davila J, , Holcik M, Majewski J, Richer J, Boycott KM
Human molecular genetics24(22)6293-300(2015 Nov)

G-actin provides substrate-specificity to eukaryotic initiation factor 2? holophosphatases.
Chen R, Rato C, Yan Y, Crespillo-Casado A, Clarke HJ, Harding HP, Marciniak SJ, Read RJ, Ron D
eLife4(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the first homozygous mutation in the PPP1R15B gene encoding the regulatory subunit of an eIF2alpha-specific phosphatase in two siblings affected by a novel syndrome of diabetes of youth with short stature, intellectual disability, and microcephaly
Title: A Missense Mutation in PPP1R15B Causes a Syndrome Including Diabetes, Short Stature, and Microcephaly.

Whole-exome sequencing identified a homozygous missense mutation, c.1972G>A; p.Arg658Cys, in protein phosphatase 1, regulatory subunit 15b (PPP1R15B)
Title: Homozygous mutation in the eukaryotic translation initiation factor 2alpha phosphatase gene, PPP1R15B, is associated with severe microcephaly, short stature and intellectual disability.

CIP2A regulates cancer metabolism and CREB phosphorylation in non-small cell lung cancer
Title: CIP2A regulates cancer metabolism and CREB phosphorylation in non-small cell lung cancer.

the mammalian traffic machinery co-opts p-eIF2alpha and CReP, regulators of translation initiation.
Title: A subunit of eukaryotic translation initiation factor 2?-phosphatase (CreP/PPP1R15B) regulates membrane traffic.

The following PPP1R15B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PPP1R15B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu16126	NM_032833.4 Latest version!	Homo sapiens protein phosphatase 1 regulatory subunit 15B (PPP1R15B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu34759	XM_005245551.5 Latest version!	Homo sapiens protein phosphatase 1 regulatory subunit 15B (PPP1R15B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OHu34759	XM_005245551.4	Homo sapiens protein phosphatase 1 regulatory subunit 15B (PPP1R15B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PPP1R15B ( NM_032833.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_116222.4 Homo sapiens protein phosphatase 1 regulatory subunit 15B (PPP1R15B), mRNA.

Price & Availability↑

CloneID	OHu16126
Clone ID Related Accession (Same CDS sequence)	NM_032833.4
Accession Version	NM_032833.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	protein phosphatase 1 regulatory subunit 15B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL606489.26 and KF459739.1. This sequence is a reference standard in the RefSeqGene project. On Apr 18, 2014 this sequence version replaced NM_032833.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK027650.1, SRR1803613.212816.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein regulatory uORF :: PMID: 25621764 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGCCGG GGACAGGCGG ATCGCGGAAA CGGCTTGGCC CTCGGGCGGG CTTCCGGTTC 
TGGCCACCCT TTTTCCCTCG GCGATCGCAA GCAGGCTCTT CTAAGTTCCC GACGCCTCTT 
GGCCCGGAAA ACTCCGGGAA CCCCACACTG CTTTCCTCTG CCCAGCCCGA GACTCGGGTC 
AGTTACTGGA CGAAACTGCT CTCCCAGCTC CTTGCGCCGC TCCCCGGATT GCTTCAGAAG 
GTGCTAATTT GGAGCCAACT TTTCGGTGGA ATGTTTCCGA CCAGATGGCT AGATTTTGCT 
GGAGTCTACA GCGCCCTGAG AGCCCTGAAG GGACGGGAGA AACCAGCCGC CCCCACAGCG 
CAGAAATCTT TGAGTTCGCT GCAGCTCGAC TCCTCAGACC CCTCGGTCAC CAGTCCCCTT 
GATTGGCTAG AGGAGGGGAT CCACTGGCAA TACTCGCCCC CAGACCTAAA ATTGGAGCTT 
AAGGCCAAGG GAAGTGCTTT GGACCCTGCA GCACAGGCTT TTCTCTTAGA GCAGCAGCTG 
TGGGGAGTGG AGCTGTTGCC CAGTAGCCTT CAATCCCGTC TGTACTCTAA CCGGGAACTT 
GGCTCTTCGC CCTCTGGGCC TCTAAACATT CAACGCATAG ACAATTTCAG TGTGGTATCC 
TATTTGCTGA ACCCTTCCTA CCTGGACTGC TTTCCTAGGC TAGAAGTCAG CTATCAGAAC 
AGTGATGGAA ATAGCGAGGT AGTCGGCTTC CAGACACTAA CCCCAGAGAG CAGCTGCCTG 
AGAGAGGACC ATTGTCATCC CCAGCCGCTG AGTGCAGAAC TCATTCCGGC CTCGTGGCAG 
GGATGTCCAC CTCTTTCTAC GGAAGGCCTA CCAGAAATTC ACCATCTTCG CATGAAACGG 
CTGGAATTCC TTCAACAGGC TAGCAAGGGG CAAGATTTAC CCACCCCTGA CCAGGATAAT 
GGCTACCACA GCCTGGAGGA GGAACACAGC CTTCTCCGGA TGGATCCAAA ACACTGCAGA 
GATAACCCAA CACAGTTTGT TCCTGCTGCT GGAGACATTC CTGGAAACAC CCAGGAATCC 
ACTGAAGAAA AAATAGAATT ATTAACTACA GAGGTTCCAC TTGCTTTGGA AGAAGAGAGC 
CCTTCTGAGG GCTGTCCATC TAGTGAGATA CCTATGGAAA AGGAGCCTGG AGAGGGCCGA 
ATAAGTGTAG TTGATTACTC ATACCTAGAA GGTGACCTTC CCATTTCTGC CAGACCAGCT 
TGTAGTAACA AACTGATAGA TTATATTTTG GGAGGTGCAT CCAGTGACCT GGAAACAAGT 
TCTGATCCAG AAGGTGAGGA TTGGGATGAG GAAGCTGAGG ATGATGGTTT TGATAGTGAT 
AGCTCACTGT CAGACTCAGA CCTTGAACAA GACCCTGAAG GGCTTCACCT TTGGAACTCT 
TTCTGCAGTG TAGATCCTTA TAATCCCCAG AACTTTACAG CAACAATTCA GACTGCTGCC 
AGAATTGTTC CTGAAGAGCC TTCTGATTCA GAGAAGGATT TGTCTGGCAA GTCTGATCTA 
GAGAATTCCT CCCAGTCTGG AAGCCTTCCT GAGACCCCTG AGCATAGTTC TGGGGAGGAA 
GATGACTGGG AATCTAGTGC AGATGAAGCA GAGAGTCTCA AACTGTGGAA CTCATTCTGT 
AATTCTGATG ACCCCTACAA CCCTTTAAAT TTTAAGGCTC CTTTTCAAAC ATCAGGGGAA 
AATGAGAAAG GCTGTCGTGA CTCAAAGACC CCATCTGAGT CCATTGTGGC CATTTCTGAG 
TGTCACACCT TACTTTCTTG TAAGGTGCAG CTGTTGGGGA GCCAAGAAAG TGAATGTCCA 
GACTCGGTAC AGCGTGACGT TCTTTCTGGA GGAAGACACA CACATGTCAA AAGAAAAAAG 
GTAACCTTCC TTGAAGAAGT TACTGAGTAT TATATAAGTG GTGATGAGGA TCGCAAAGGA 
CCATGGGAAG AATTTGCAAG GGATGGATGC AGGTTCCAGA AACGAATTCA AGAAACAGAA 
GATGCTATTG GATATTGCTT GACATTTGAA CACAGAGAAA GAATGTTTAA TAGACTCCAG 
GGAACATGCT TCAAAGGACT TAATGTTCTC AAGCAATGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116222.4
CDS	407..2548
Translation	MEPGTGGSRKRLGPRAGFRFWPPFFPRRSQAGSSKFPTPLGPENSGNPTLLSSAQPETRVSYWTKLLSQLLAPLPGLLQKVLIWSQLFGGMFPTRWLDFAGVYSALRALKGREKPAAPTAQKSLSSLQLDSSDPSVTSPLDWLEEGIHWQYSPPDLKLELKAKGSALDPAAQAFLLEQQLWGVELLPSSLQSRLYSNRELGSSPSGPLNIQRIDNFSVVSYLLNPSYLDCFPRLEVSYQNSDGNSEVVGFQTLTPESSCLREDHCHPQPLSAELIPASWQGCPPLSTEGLPEIHHLRMKRLEFLQQASKGQDLPTPDQDNGYHSLEEEHSLLRMDPKHCRDNPTQFVPAAGDIPGNTQESTEEKIELLTTEVPLALEEESPSEGCPSSEIPMEKEPGEGRISVVDYSYLEGDLPISARPACSNKLIDYILGGASSDLETSSDPEGEDWDEEAEDDGFDSDSSLSDSDLEQDPEGLHLWNSFCSVDPYNPQNFTATIQTAARIVPEEPSDSEKDLSGKSDLENSSQSGSLPETPEHSSGEEDDWESSADEAESLKLWNSFCNSDDPYNPLNFKAPFQTSGENEKGCRDSKTPSESIVAISECHTLLSCKVQLLGSQESECPDSVQRDVLSGGRHTHVKRKKVTFLEEVTEYYISGDEDRKGPWEEFARDGCRFQKRIQETEDAIGYCLTFEHRERMFNRLQGTCFKGLNVLKQC

Target ORF information:

RefSeq Version	NM_032833.4
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 1 regulatory subunit 15B (PPP1R15B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032833.4

ORF Insert Sequence:

ATGGAGCCGG GGACAGGCGG ATCGCGGAAA CGGCTTGGCC CTCGGGCGGG CTTCCGGTTC 
TGGCCACCCT TTTTCCCTCG GCGATCGCAA GCAGGCTCTT CTAAGTTCCC GACGCCTCTT 
GGCCCGGAAA ACTCCGGGAA CCCCACACTG CTTTCCTCTG CCCAGCCCGA GACTCGGGTC 
AGTTACTGGA CGAAACTGCT CTCCCAGCTC CTTGCGCCGC TCCCCGGATT GCTTCAGAAG 
GTGCTAATTT GGAGCCAACT TTTCGGTGGA ATGTTTCCGA CCAGATGGCT AGATTTTGCT 
GGAGTCTACA GCGCCCTGAG AGCCCTGAAG GGACGGGAGA AACCAGCCGC CCCCACAGCG 
CAGAAATCTT TGAGTTCGCT GCAGCTCGAC TCCTCAGACC CCTCGGTCAC CAGTCCCCTT 
GATTGGCTAG AGGAGGGGAT CCACTGGCAA TACTCGCCCC CAGACCTAAA ATTGGAGCTT 
AAGGCCAAGG GAAGTGCTTT GGACCCTGCA GCACAGGCTT TTCTCTTAGA GCAGCAGCTG 
TGGGGAGTGG AGCTGTTGCC CAGTAGCCTT CAATCCCGTC TGTACTCTAA CCGGGAACTT 
GGCTCTTCGC CCTCTGGGCC TCTAAACATT CAACGCATAG ACAATTTCAG TGTGGTATCC 
TATTTGCTGA ACCCTTCCTA CCTGGACTGC TTTCCTAGGC TAGAAGTCAG CTATCAGAAC 
AGTGATGGAA ATAGCGAGGT AGTCGGCTTC CAGACACTAA CCCCAGAGAG CAGCTGCCTG 
AGAGAGGACC ATTGTCATCC CCAGCCGCTG AGTGCAGAAC TCATTCCGGC CTCGTGGCAG 
GGATGTCCAC CTCTTTCTAC GGAAGGCCTA CCAGAAATTC ACCATCTTCG CATGAAACGG 
CTGGAATTCC TTCAACAGGC TAGCAAGGGG CAAGATTTAC CCACCCCTGA CCAGGATAAT 
GGCTACCACA GCCTGGAGGA GGAACACAGC CTTCTCCGGA TGGATCCAAA ACACTGCAGA 
GATAACCCAA CACAGTTTGT TCCTGCTGCT GGAGACATTC CTGGAAACAC CCAGGAATCC 
ACTGAAGAAA AAATAGAATT ATTAACTACA GAGGTTCCAC TTGCTTTGGA AGAAGAGAGC 
CCTTCTGAGG GCTGTCCATC TAGTGAGATA CCTATGGAAA AGGAGCCTGG AGAGGGCCGA 
ATAAGTGTAG TTGATTACTC ATACCTAGAA GGTGACCTTC CCATTTCTGC CAGACCAGCT 
TGTAGTAACA AACTGATAGA TTATATTTTG GGAGGTGCAT CCAGTGACCT GGAAACAAGT 
TCTGATCCAG AAGGTGAGGA TTGGGATGAG GAAGCTGAGG ATGATGGTTT TGATAGTGAT 
AGCTCACTGT CAGACTCAGA CCTTGAACAA GACCCTGAAG GGCTTCACCT TTGGAACTCT 
TTCTGCAGTG TAGATCCTTA TAATCCCCAG AACTTTACAG CAACAATTCA GACTGCTGCC 
AGAATTGTTC CTGAAGAGCC TTCTGATTCA GAGAAGGATT TGTCTGGCAA GTCTGATCTA 
GAGAATTCCT CCCAGTCTGG AAGCCTTCCT GAGACCCCTG AGCATAGTTC TGGGGAGGAA 
GATGACTGGG AATCTAGTGC AGATGAAGCA GAGAGTCTCA AACTGTGGAA CTCATTCTGT 
AATTCTGATG ACCCCTACAA CCCTTTAAAT TTTAAGGCTC CTTTTCAAAC ATCAGGGGAA 
AATGAGAAAG GCTGTCGTGA CTCAAAGACC CCATCTGAGT CCATTGTGGC CATTTCTGAG 
TGTCACACCT TACTTTCTTG TAAGGTGCAG CTGTTGGGGA GCCAAGAAAG TGAATGTCCA 
GACTCGGTAC AGCGTGACGT TCTTTCTGGA GGAAGACACA CACATGTCAA AAGAAAAAAG 
GTAACCTTCC TTGAAGAAGT TACTGAGTAT TATATAAGTG GTGATGAGGA TCGCAAAGGA 
CCATGGGAAG AATTTGCAAG GGATGGATGC AGGTTCCAGA AACGAATTCA AGAAACAGAA 
GATGCTATTG GATATTGCTT GACATTTGAA CACAGAGAAA GAATGTTTAA TAGACTCCAG 
GGAACATGCT TCAAAGGACT TAATGTTCTC AAGCAATGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP1R15B ( XM_005245551.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245608.2 Homo sapiens protein phosphatase 1 regulatory subunit 15B (PPP1R15B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu34759
Clone ID Related Accession (Same CDS sequence)	XM_005245551.5 , XM_005245551.4
Accession Version	XM_005245551.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein phosphatase 1 regulatory subunit 15B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005245551.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGG GGACAGGCGG ATCGCGGAAA CGGCTTGGCC CTCGGGCGGG CTTCCGGTTC 
TGGCCACCCT TTTTCCCTCG GCGATCGCAA GCAGGCTCTT CTAAGTTCCC GACGCCTCTT 
GGCCCGGAAA ACTCCGGGAA CCCCACACTG CTTTCCTCTG CCCAGCCCGA GACTCGGGTC 
AGTTACTGGA CGAAACTGCT CTCCCAGCTC CTTGCGCCGC TCCCCGGATT GCTTCAGAAG 
GTGCTAATTT GGAGCCAACT TTTCGGTGGA ATGTTTCCGA CCAGATGGCT AGATTTTGCT 
GGAGTCTACA GCGCCCTGAG AGCCCTGAAG GGACGGGAGA AACCAGCCGC CCCCACAGCG 
CAGAAATCTT TGAGTTCGCT GCAGCTCGAC TCCTCAGACC CCTCGGTCAC CAGTCCCCTT 
GATTGGCTAG AGGAGGGGAT CCACTGGCAA TACTCGCCCC CAGACCTAAA ATTGGAGCTT 
AAGGCCAAGG GAAGTGCTTT GGACCCTGCA GCACAGGCTT TTCTCTTAGA GCAGCAGCTG 
TGGGGAGTGG AGCTGTTGCC CAGTAGCCTT CAATCCCGTC TGTACTCTAA CCGGGAACTT 
GGCTCTTCGC CCTCTGGGCC TCTAAACATT CAACGCATAG ACAATTTCAG TGTGGTATCC 
TATTTGCTGA ACCCTTCCTA CCTGGACTGC TTTCCTAGGC TAGAAGTCAG CTATCAGAAC 
AGTGATGGAA ATAGCGAGGT AGTCGGCTTC CAGACACTAA CCCCAGAGAG CAGCTGCCTG 
AGAGAGGACC ATTGTCATCC CCAGCCGCTG AGTGCAGAAC TCATTCCGGC CTCGTGGCAG 
GGATGTCCAC CTCTTTCTAC GGAAGGCCTA CCAGAAATTC ACCATCTTCG CATGAAACGG 
CTGGAATTCC TTCAACAGGC TAGCAAGGGG CAAGATTTAC CCACCCCTGA CCAGGATAAT 
GGCTACCACA GCCTGGAGGA GGAACACAGC CTTCTCCGGA TGGATCCAAA ACACTGCAGA 
GATAACCCAA CACAGTTTGT TCCTGCTGCT GGAGACATTC CTGGAAACAC CCAGGAATCC 
ACTGAAGAAA AAATAGAATT ATTAACTACA GAGGTTCCAC TTGCTTTGGA AGAAGAGAGC 
CCTTCTGAGG GCTGTCCATC TAGTGAGATA CCTATGGAAA AGGAGCCTGG AGAGGGCCGA 
ATAAGTGTAG TTGATTACTC ATACCTAGAA GGTGACCTTC CCATTTCTGC CAGACCAGCT 
TGTAGTAACA AACTGATAGA TTATATTTTG GGAGGTGCAT CCAGTGACCT GGAAACAAGT 
TCTGATCCAG AAGGTGAGGA TTGGGATGAG GAAGCTGAGG ATGATGGTTT TGATAGTGAT 
AGCTCACTGT CAGACTCAGA CCTTGAACAA GACCCTGAAG GGCTTCACCT TTGGAACTCT 
TTCTGCAGTG TAGATCCTTA TAATCCCCAG AACTTTACAG CAACAATTCA GACTGCTGCC 
AGAATTGTTC CTGAAGAGCC TTCTGATTCA GAGAAGGATT TGTCTGGCAA GTCTGATCTA 
GAGAATTCCT CCCAGTCTGG AAGCCTTCCT GAGACCCCTG AGCATAGTTC TGGGGAGGAA 
GATGACTGGG AATCTAGTGC AGATGAAGCA GAGAGTCTCA AACTGTGGAA CTCATTCTGT 
AATTCTGATG ACCCCTACAA CCCTTTAAAT TTTAAGGCTC CTTTTCAAAC ATCAGGGGAA 
AATGAGAAAG GCTGTCGTGA CTCAAAGACC CCATCTGAGT CCATTGTGGC CATTTCTGAG 
TGTCACACCT TACTTTCTTG TAAGGTGCAG CTGTTGGGGA GCCAAGAAAG TGAATGTCCA 
GACTCGGTAC AGCGTGACGT TCTTTCTGGA GGAAGACACA CACATGTCAA AAGAAAAAAG 
ATCCCTACCA AGAAGAAGGA TGAAGAACTT AATAGCAAAA GAAGAGCACA ATTGCCAGAG 
ATGCGAGAAG AAAGGACCTC ACACGGTTCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245608.2
CDS	1044..3056
Translation	MEPGTGGSRKRLGPRAGFRFWPPFFPRRSQAGSSKFPTPLGPENSGNPTLLSSAQPETRVSYWTKLLSQLLAPLPGLLQKVLIWSQLFGGMFPTRWLDFAGVYSALRALKGREKPAAPTAQKSLSSLQLDSSDPSVTSPLDWLEEGIHWQYSPPDLKLELKAKGSALDPAAQAFLLEQQLWGVELLPSSLQSRLYSNRELGSSPSGPLNIQRIDNFSVVSYLLNPSYLDCFPRLEVSYQNSDGNSEVVGFQTLTPESSCLREDHCHPQPLSAELIPASWQGCPPLSTEGLPEIHHLRMKRLEFLQQASKGQDLPTPDQDNGYHSLEEEHSLLRMDPKHCRDNPTQFVPAAGDIPGNTQESTEEKIELLTTEVPLALEEESPSEGCPSSEIPMEKEPGEGRISVVDYSYLEGDLPISARPACSNKLIDYILGGASSDLETSSDPEGEDWDEEAEDDGFDSDSSLSDSDLEQDPEGLHLWNSFCSVDPYNPQNFTATIQTAARIVPEEPSDSEKDLSGKSDLENSSQSGSLPETPEHSSGEEDDWESSADEAESLKLWNSFCNSDDPYNPLNFKAPFQTSGENEKGCRDSKTPSESIVAISECHTLLSCKVQLLGSQESECPDSVQRDVLSGGRHTHVKRKKIPTKKKDEELNSKRRAQLPEMREERTSHGS

Target ORF information:

RefSeq Version	XM_005245551.5
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 1 regulatory subunit 15B (PPP1R15B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005245551.5

ORF Insert Sequence:

ATGGAGCCGG GGACAGGCGG ATCGCGGAAA CGGCTTGGCC CTCGGGCGGG CTTCCGGTTC 
TGGCCACCCT TTTTCCCTCG GCGATCGCAA GCAGGCTCTT CTAAGTTCCC GACGCCTCTT 
GGCCCGGAAA ACTCCGGGAA CCCCACACTG CTTTCCTCTG CCCAGCCCGA GACTCGGGTC 
AGTTACTGGA CGAAACTGCT CTCCCAGCTC CTTGCGCCGC TCCCCGGATT GCTTCAGAAG 
GTGCTAATTT GGAGCCAACT TTTCGGTGGA ATGTTTCCGA CCAGATGGCT AGATTTTGCT 
GGAGTCTACA GCGCCCTGAG AGCCCTGAAG GGACGGGAGA AACCAGCCGC CCCCACAGCG 
CAGAAATCTT TGAGTTCGCT GCAGCTCGAC TCCTCAGACC CCTCGGTCAC CAGTCCCCTT 
GATTGGCTAG AGGAGGGGAT CCACTGGCAA TACTCGCCCC CAGACCTAAA ATTGGAGCTT 
AAGGCCAAGG GAAGTGCTTT GGACCCTGCA GCACAGGCTT TTCTCTTAGA GCAGCAGCTG 
TGGGGAGTGG AGCTGTTGCC CAGTAGCCTT CAATCCCGTC TGTACTCTAA CCGGGAACTT 
GGCTCTTCGC CCTCTGGGCC TCTAAACATT CAACGCATAG ACAATTTCAG TGTGGTATCC 
TATTTGCTGA ACCCTTCCTA CCTGGACTGC TTTCCTAGGC TAGAAGTCAG CTATCAGAAC 
AGTGATGGAA ATAGCGAGGT AGTCGGCTTC CAGACACTAA CCCCAGAGAG CAGCTGCCTG 
AGAGAGGACC ATTGTCATCC CCAGCCGCTG AGTGCAGAAC TCATTCCGGC CTCGTGGCAG 
GGATGTCCAC CTCTTTCTAC GGAAGGCCTA CCAGAAATTC ACCATCTTCG CATGAAACGG 
CTGGAATTCC TTCAACAGGC TAGCAAGGGG CAAGATTTAC CCACCCCTGA CCAGGATAAT 
GGCTACCACA GCCTGGAGGA GGAACACAGC CTTCTCCGGA TGGATCCAAA ACACTGCAGA 
GATAACCCAA CACAGTTTGT TCCTGCTGCT GGAGACATTC CTGGAAACAC CCAGGAATCC 
ACTGAAGAAA AAATAGAATT ATTAACTACA GAGGTTCCAC TTGCTTTGGA AGAAGAGAGC 
CCTTCTGAGG GCTGTCCATC TAGTGAGATA CCTATGGAAA AGGAGCCTGG AGAGGGCCGA 
ATAAGTGTAG TTGATTACTC ATACCTAGAA GGTGACCTTC CCATTTCTGC CAGACCAGCT 
TGTAGTAACA AACTGATAGA TTATATTTTG GGAGGTGCAT CCAGTGACCT GGAAACAAGT 
TCTGATCCAG AAGGTGAGGA TTGGGATGAG GAAGCTGAGG ATGATGGTTT TGATAGTGAT 
AGCTCACTGT CAGACTCAGA CCTTGAACAA GACCCTGAAG GGCTTCACCT TTGGAACTCT 
TTCTGCAGTG TAGATCCTTA TAATCCCCAG AACTTTACAG CAACAATTCA GACTGCTGCC 
AGAATTGTTC CTGAAGAGCC TTCTGATTCA GAGAAGGATT TGTCTGGCAA GTCTGATCTA 
GAGAATTCCT CCCAGTCTGG AAGCCTTCCT GAGACCCCTG AGCATAGTTC TGGGGAGGAA 
GATGACTGGG AATCTAGTGC AGATGAAGCA GAGAGTCTCA AACTGTGGAA CTCATTCTGT 
AATTCTGATG ACCCCTACAA CCCTTTAAAT TTTAAGGCTC CTTTTCAAAC ATCAGGGGAA 
AATGAGAAAG GCTGTCGTGA CTCAAAGACC CCATCTGAGT CCATTGTGGC CATTTCTGAG 
TGTCACACCT TACTTTCTTG TAAGGTGCAG CTGTTGGGGA GCCAAGAAAG TGAATGTCCA 
GACTCGGTAC AGCGTGACGT TCTTTCTGGA GGAAGACACA CACATGTCAA AAGAAAAAAG 
ATCCCTACCA AGAAGAAGGA TGAAGAACTT AATAGCAAAA GAAGAGCACA ATTGCCAGAG 
ATGCGAGAAG AAAGGACCTC ACACGGTTCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PPP1R15B ( XM_005245551.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005245608.2 Homo sapiens protein phosphatase 1 regulatory subunit 15B (PPP1R15B), transcript variant X1, mRNA.

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CloneID	OHu34759
Clone ID Related Accession (Same CDS sequence)	XM_005245551.5 , XM_005245551.4
Accession Version	XM_005245551.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	protein phosphatase 1 regulatory subunit 15B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005245551.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCCGG GGACAGGCGG ATCGCGGAAA CGGCTTGGCC CTCGGGCGGG CTTCCGGTTC 
TGGCCACCCT TTTTCCCTCG GCGATCGCAA GCAGGCTCTT CTAAGTTCCC GACGCCTCTT 
GGCCCGGAAA ACTCCGGGAA CCCCACACTG CTTTCCTCTG CCCAGCCCGA GACTCGGGTC 
AGTTACTGGA CGAAACTGCT CTCCCAGCTC CTTGCGCCGC TCCCCGGATT GCTTCAGAAG 
GTGCTAATTT GGAGCCAACT TTTCGGTGGA ATGTTTCCGA CCAGATGGCT AGATTTTGCT 
GGAGTCTACA GCGCCCTGAG AGCCCTGAAG GGACGGGAGA AACCAGCCGC CCCCACAGCG 
CAGAAATCTT TGAGTTCGCT GCAGCTCGAC TCCTCAGACC CCTCGGTCAC CAGTCCCCTT 
GATTGGCTAG AGGAGGGGAT CCACTGGCAA TACTCGCCCC CAGACCTAAA ATTGGAGCTT 
AAGGCCAAGG GAAGTGCTTT GGACCCTGCA GCACAGGCTT TTCTCTTAGA GCAGCAGCTG 
TGGGGAGTGG AGCTGTTGCC CAGTAGCCTT CAATCCCGTC TGTACTCTAA CCGGGAACTT 
GGCTCTTCGC CCTCTGGGCC TCTAAACATT CAACGCATAG ACAATTTCAG TGTGGTATCC 
TATTTGCTGA ACCCTTCCTA CCTGGACTGC TTTCCTAGGC TAGAAGTCAG CTATCAGAAC 
AGTGATGGAA ATAGCGAGGT AGTCGGCTTC CAGACACTAA CCCCAGAGAG CAGCTGCCTG 
AGAGAGGACC ATTGTCATCC CCAGCCGCTG AGTGCAGAAC TCATTCCGGC CTCGTGGCAG 
GGATGTCCAC CTCTTTCTAC GGAAGGCCTA CCAGAAATTC ACCATCTTCG CATGAAACGG 
CTGGAATTCC TTCAACAGGC TAGCAAGGGG CAAGATTTAC CCACCCCTGA CCAGGATAAT 
GGCTACCACA GCCTGGAGGA GGAACACAGC CTTCTCCGGA TGGATCCAAA ACACTGCAGA 
GATAACCCAA CACAGTTTGT TCCTGCTGCT GGAGACATTC CTGGAAACAC CCAGGAATCC 
ACTGAAGAAA AAATAGAATT ATTAACTACA GAGGTTCCAC TTGCTTTGGA AGAAGAGAGC 
CCTTCTGAGG GCTGTCCATC TAGTGAGATA CCTATGGAAA AGGAGCCTGG AGAGGGCCGA 
ATAAGTGTAG TTGATTACTC ATACCTAGAA GGTGACCTTC CCATTTCTGC CAGACCAGCT 
TGTAGTAACA AACTGATAGA TTATATTTTG GGAGGTGCAT CCAGTGACCT GGAAACAAGT 
TCTGATCCAG AAGGTGAGGA TTGGGATGAG GAAGCTGAGG ATGATGGTTT TGATAGTGAT 
AGCTCACTGT CAGACTCAGA CCTTGAACAA GACCCTGAAG GGCTTCACCT TTGGAACTCT 
TTCTGCAGTG TAGATCCTTA TAATCCCCAG AACTTTACAG CAACAATTCA GACTGCTGCC 
AGAATTGTTC CTGAAGAGCC TTCTGATTCA GAGAAGGATT TGTCTGGCAA GTCTGATCTA 
GAGAATTCCT CCCAGTCTGG AAGCCTTCCT GAGACCCCTG AGCATAGTTC TGGGGAGGAA 
GATGACTGGG AATCTAGTGC AGATGAAGCA GAGAGTCTCA AACTGTGGAA CTCATTCTGT 
AATTCTGATG ACCCCTACAA CCCTTTAAAT TTTAAGGCTC CTTTTCAAAC ATCAGGGGAA 
AATGAGAAAG GCTGTCGTGA CTCAAAGACC CCATCTGAGT CCATTGTGGC CATTTCTGAG 
TGTCACACCT TACTTTCTTG TAAGGTGCAG CTGTTGGGGA GCCAAGAAAG TGAATGTCCA 
GACTCGGTAC AGCGTGACGT TCTTTCTGGA GGAAGACACA CACATGTCAA AAGAAAAAAG 
ATCCCTACCA AGAAGAAGGA TGAAGAACTT AATAGCAAAA GAAGAGCACA ATTGCCAGAG 
ATGCGAGAAG AAAGGACCTC ACACGGTTCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005245608.2
CDS	470..2482
Translation	MEPGTGGSRKRLGPRAGFRFWPPFFPRRSQAGSSKFPTPLGPENSGNPTLLSSAQPETRVSYWTKLLSQLLAPLPGLLQKVLIWSQLFGGMFPTRWLDFAGVYSALRALKGREKPAAPTAQKSLSSLQLDSSDPSVTSPLDWLEEGIHWQYSPPDLKLELKAKGSALDPAAQAFLLEQQLWGVELLPSSLQSRLYSNRELGSSPSGPLNIQRIDNFSVVSYLLNPSYLDCFPRLEVSYQNSDGNSEVVGFQTLTPESSCLREDHCHPQPLSAELIPASWQGCPPLSTEGLPEIHHLRMKRLEFLQQASKGQDLPTPDQDNGYHSLEEEHSLLRMDPKHCRDNPTQFVPAAGDIPGNTQESTEEKIELLTTEVPLALEEESPSEGCPSSEIPMEKEPGEGRISVVDYSYLEGDLPISARPACSNKLIDYILGGASSDLETSSDPEGEDWDEEAEDDGFDSDSSLSDSDLEQDPEGLHLWNSFCSVDPYNPQNFTATIQTAARIVPEEPSDSEKDLSGKSDLENSSQSGSLPETPEHSSGEEDDWESSADEAESLKLWNSFCNSDDPYNPLNFKAPFQTSGENEKGCRDSKTPSESIVAISECHTLLSCKVQLLGSQESECPDSVQRDVLSGGRHTHVKRKKIPTKKKDEELNSKRRAQLPEMREERTSHGS

Target ORF information:

RefSeq Version	XM_005245551.4
Organism	Homo sapiens(human)
Definition	Homo sapiens protein phosphatase 1 regulatory subunit 15B (PPP1R15B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005245551.4

ORF Insert Sequence:

ATGGAGCCGG GGACAGGCGG ATCGCGGAAA CGGCTTGGCC CTCGGGCGGG CTTCCGGTTC 
TGGCCACCCT TTTTCCCTCG GCGATCGCAA GCAGGCTCTT CTAAGTTCCC GACGCCTCTT 
GGCCCGGAAA ACTCCGGGAA CCCCACACTG CTTTCCTCTG CCCAGCCCGA GACTCGGGTC 
AGTTACTGGA CGAAACTGCT CTCCCAGCTC CTTGCGCCGC TCCCCGGATT GCTTCAGAAG 
GTGCTAATTT GGAGCCAACT TTTCGGTGGA ATGTTTCCGA CCAGATGGCT AGATTTTGCT 
GGAGTCTACA GCGCCCTGAG AGCCCTGAAG GGACGGGAGA AACCAGCCGC CCCCACAGCG 
CAGAAATCTT TGAGTTCGCT GCAGCTCGAC TCCTCAGACC CCTCGGTCAC CAGTCCCCTT 
GATTGGCTAG AGGAGGGGAT CCACTGGCAA TACTCGCCCC CAGACCTAAA ATTGGAGCTT 
AAGGCCAAGG GAAGTGCTTT GGACCCTGCA GCACAGGCTT TTCTCTTAGA GCAGCAGCTG 
TGGGGAGTGG AGCTGTTGCC CAGTAGCCTT CAATCCCGTC TGTACTCTAA CCGGGAACTT 
GGCTCTTCGC CCTCTGGGCC TCTAAACATT CAACGCATAG ACAATTTCAG TGTGGTATCC 
TATTTGCTGA ACCCTTCCTA CCTGGACTGC TTTCCTAGGC TAGAAGTCAG CTATCAGAAC 
AGTGATGGAA ATAGCGAGGT AGTCGGCTTC CAGACACTAA CCCCAGAGAG CAGCTGCCTG 
AGAGAGGACC ATTGTCATCC CCAGCCGCTG AGTGCAGAAC TCATTCCGGC CTCGTGGCAG 
GGATGTCCAC CTCTTTCTAC GGAAGGCCTA CCAGAAATTC ACCATCTTCG CATGAAACGG 
CTGGAATTCC TTCAACAGGC TAGCAAGGGG CAAGATTTAC CCACCCCTGA CCAGGATAAT 
GGCTACCACA GCCTGGAGGA GGAACACAGC CTTCTCCGGA TGGATCCAAA ACACTGCAGA 
GATAACCCAA CACAGTTTGT TCCTGCTGCT GGAGACATTC CTGGAAACAC CCAGGAATCC 
ACTGAAGAAA AAATAGAATT ATTAACTACA GAGGTTCCAC TTGCTTTGGA AGAAGAGAGC 
CCTTCTGAGG GCTGTCCATC TAGTGAGATA CCTATGGAAA AGGAGCCTGG AGAGGGCCGA 
ATAAGTGTAG TTGATTACTC ATACCTAGAA GGTGACCTTC CCATTTCTGC CAGACCAGCT 
TGTAGTAACA AACTGATAGA TTATATTTTG GGAGGTGCAT CCAGTGACCT GGAAACAAGT 
TCTGATCCAG AAGGTGAGGA TTGGGATGAG GAAGCTGAGG ATGATGGTTT TGATAGTGAT 
AGCTCACTGT CAGACTCAGA CCTTGAACAA GACCCTGAAG GGCTTCACCT TTGGAACTCT 
TTCTGCAGTG TAGATCCTTA TAATCCCCAG AACTTTACAG CAACAATTCA GACTGCTGCC 
AGAATTGTTC CTGAAGAGCC TTCTGATTCA GAGAAGGATT TGTCTGGCAA GTCTGATCTA 
GAGAATTCCT CCCAGTCTGG AAGCCTTCCT GAGACCCCTG AGCATAGTTC TGGGGAGGAA 
GATGACTGGG AATCTAGTGC AGATGAAGCA GAGAGTCTCA AACTGTGGAA CTCATTCTGT 
AATTCTGATG ACCCCTACAA CCCTTTAAAT TTTAAGGCTC CTTTTCAAAC ATCAGGGGAA 
AATGAGAAAG GCTGTCGTGA CTCAAAGACC CCATCTGAGT CCATTGTGGC CATTTCTGAG 
TGTCACACCT TACTTTCTTG TAAGGTGCAG CTGTTGGGGA GCCAAGAAAG TGAATGTCCA 
GACTCGGTAC AGCGTGACGT TCTTTCTGGA GGAAGACACA CACATGTCAA AAGAAAAAAG 
ATCCCTACCA AGAAGAAGGA TGAAGAACTT AATAGCAAAA GAAGAGCACA ATTGCCAGAG 
ATGCGAGAAG AAAGGACCTC ACACGGTTCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Systematic Analysis of Human Protein Phosphatase Interactions and Dynamics.
Cell systems4(4)430-444.e5(2017 Apr)
Yadav L,Tamene F,G??s H,van Drogen A,Katainen R,Aebersold R,Gstaiger M,Varjosalo M

A Missense Mutation in PPP1R15B Causes a Syndrome Including Diabetes, Short Stature, and Microcephaly.
Diabetes64(11)3951-62(2015 Nov)
Abdulkarim B,Nicolino M,Igoillo-Esteve M,Daures M,Romero S,Philippi A,Sen?e V,Lopes M,Cunha DA,Harding HP,Derbois C,Bendelac N,Hattersley AT,Eizirik DL,Ron D,Cnop M,Julier C

Homozygous mutation in the eukaryotic translation initiation factor 2alpha phosphatase gene, PPP1R15B, is associated with severe microcephaly, short stature and intellectual disability.
Human molecular genetics24(22)6293-300(2015 Nov)
Kernohan KD,T?treault M,Liwak-Muir U,Geraghty MT,Qin W,Venkateswaran S,Davila J,,Holcik M,Majewski J,Richer J,Boycott KM

G-actin provides substrate-specificity to eukaryotic initiation factor 2? holophosphatases.
eLife4(2015 Mar)
Chen R,Rato C,Yan Y,Crespillo-Casado A,Clarke HJ,Harding HP,Marciniak SJ,Read RJ,Ron D

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PPP1R15B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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