CEP89 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CEP89
Entrez Gene ID	84902
Full Name	centrosomal protein 89
Synonyms	CCDC123,CEP123
General protein information	Preferred Names centrosomal protein 89 Names centrosomal protein of 89 kDa centrosomal protein 123 centrosomal protein 89kDa coiled-coil domain containing 123 coiled-coil domain-containing protein 123, mitochondrial
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.11
Disorder MIM:	615470

mRNA and Protein(s)

mRNA	Protein	Name
NM_032816.4	NP_116205.3	centrosomal protein of 89 kDa
XM_011527425.2	XP_011525727.1	centrosomal protein of 89 kDa isoform X3
XM_017027398.1	XP_016882887.1	centrosomal protein of 89 kDa isoform X2
XM_024451745.1	XP_024307513.1	centrosomal protein of 89 kDa isoform X3
NM_032816.5	NP_116205.3	centrosomal protein of 89 kDa
XM_005259344.3	XP_005259401.1	centrosomal protein of 89 kDa isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CEP89	XP_533706.5
Homo sapiens (human)	CEP89	NP_116205.3
Pan troglodytes (chimpanzee)	CEP89	XP_524203.4
Rattus norvegicus (Norway rat)	Cep89	NP_001121071.1
Danio rerio (zebrafish)	cep89	NP_957172.1
	CEP89	XP_002801221.1
Bos taurus (cattle)	CEP89	XP_005219104.1
Mus musculus (house mouse)	Cep89	NP_082396.1
Gallus gallus (chicken)	CEP89	XP_414131.1
Xenopus tropicalis (tropical clawed frog)	cep89	XP_004913612.1

Gene Ontology
Bibliography

Related articles in PubMed

CEP89 is required for mitochondrial metabolism and neuronal function in man and fly.
van Bon BW, Oortveld MA, Nijtmans LG, Fenckova M, Nijhof B, Besseling J, Vos M, Kramer JM, de Leeuw N, Castells-Nobau A, Asztalos L, Viragh E, Ruiter M, Hofmann F, Eshuis L, Collavin L, Huynen MA, Asztalos Z, Verstreken P, Rodenburg RJ, Smeitink JA, de Vries BB, Schenck A
Human molecular genetics22(15)3138-51(2013 Aug)

Primary ciliogenesis requires the distal appendage component Cep123.
Sillibourne JE, Hurbain I, Grand-Perret T, Goud B, Tran P, Bornens M
Biology open2(6)535-45(2013 Jun)

Centriole distal appendages promote membrane docking, leading to cilia initiation.
Tanos BE, Yang HJ, Soni R, Wang WJ, Macaluso FP, Asara JM, Tsou MF
Genes & development27(2)163-8(2013 Jan)

Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods.
Jakobsen L, Vanselow K, Skogs M, Toyoda Y, Lundberg E, Poser I, Falkenby LG, Bennetzen M, Westendorf J, Nigg EA, Uhlen M, Hyman AA, Andersen JS
The EMBO journal30(8)1520-35(2011 Apr)

Genetic loci influencing kidney function and chronic kidney disease.
Chambers JC, Zhang W, Lord GM, van der Harst P, Lawlor DA, Sehmi JS, Gale DP, Wass MN, Ahmadi KR, Bakker SJ, Beckmann J, Bilo HJ, Bochud M, Brown MJ, Caulfield MJ, Connell JM, Cook HT, Cotlarciuc I, Davey Smith G, de Silva R, Deng G, Devuyst O, Dikkeschei LD, Dimkovic N, Dockrell M, Dominiczak A, Ebrahim S, Eggermann T, Farrall M, Ferrucci L, Floege J, Forouhi NG, Gansevoort RT, Han X, Hedblad B, Homan van der Heide JJ, Hepkema BG, Hernandez-Fuentes M, Hypponen E, Johnson T, de Jong PE, Kleefstra N, Lagou V, Lapsley M, Li Y, Loos RJ, Luan J, Luttropp K, Mar?chal C, Melander O, Munroe PB, Nordfors L, Parsa A, Peltonen L, Penninx BW, Perucha E, Pouta A, Prokopenko I, Roderick PJ, Ruokonen A, Samani NJ, Sanna S, Schalling M, Schlessinger D, Schlieper G, Seelen MA, Shuldiner AR, Sj?gren M, Smit JH, Snieder H, Soranzo N, Spector TD, Stenvinkel P, Sternberg MJ, Swaminathan R, Tanaka T, Ubink-Veltmaat LJ, Uda M, Vollenweider P, Wallace C, Waterworth D, Zerres K, Waeber G, Wareham NJ, Maxwell PH, McCarthy MI, Jarvelin MR, Mooser V, Abecasis GR, Lightstone L, Scott J, Navis G, Elliott P, Kooner JS
Nature genetics42(5)373-5(2010 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CEP89 proteins play an important role in mitochondrial metabolism, especially complex IV activity, and are required for neuronal and cognitive function across evolution.
Title: CEP89 is required for mitochondrial metabolism and neuronal function in man and fly.

The following CEP89 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CEP89 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu52551	XM_024451745.1 Latest version!	Homo sapiens centrosomal protein 89 (CEP89), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu52551	XM_011527425.2 Latest version!	Homo sapiens centrosomal protein 89 (CEP89), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu73828	NM_032816.5 Latest version!	Homo sapiens centrosomal protein 89 (CEP89), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu52550	XM_005259344.3 Latest version!	Homo sapiens centrosomal protein 89 (CEP89), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu88775	XM_017027398.1 Latest version!	Homo sapiens centrosomal protein 89 (CEP89), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu73828	NM_032816.4	Homo sapiens centrosomal protein 89 (CEP89), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CEP89 ( XM_011527425.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011525727.1 Homo sapiens centrosomal protein 89 (CEP89), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu52551
Clone ID Related Accession (Same CDS sequence)	XM_011527425.2 , XM_024451745.1
Accession Version	XM_011527425.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	centrosomal protein of 89 kDa isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011527425.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTAA ACAATATGAA TCAAAGCCTT ACCCTTGAAC TAAACACAAT GAAACAAGCA 
ATGAAAGAAC TACAGTTAAA ACTTAAGGGA ATGGAAAAAG AGAAGAGAAA GCTCAAAGAG 
GCTGAGAAGG CGTCGTCACA GGAAGTTGCT GCACCTGAAT TACTTTATCT GCGAAAACAA 
GCTCAAGAAC TGGTGGATGA AAATGATGGA TTGAAAATGA CTGTCCATCG TTTGAATGTA 
GAACTCAGTC GATATCAGAC AAAATTCAGG CATTTGTCCA AGGAAGAGAG CTTAAATATT 
GAAGGCCTCC CATCCAAGGG CCCTATACCA CCCTGGTTGT TGGATATAAA GTACCTGTCA 
CCATTGTTGC TGGCTTATGA AGATATGATG AAAGAGAAGG ACGAGCTCAA TGCCACCCTC 
AAGGAGGAAA TGAGAATGTT TAGGATGCGA GTCCAAGAAG TGGTGAAAGA AAATGAAGAA 
TTGCACCAAG AGTTAAATAA GAGTAGTGCT GTTACCAGTG AGGAATGGCG TCAGCTTCAG 
ACTCAAGCAA AACTGGTTTT AGAGGAAAAC AAGTTGTTGC TGGAGCAGTT GGAGATTCAG 
CAAAGGAAAG CCAAGGACAG CCACCAGGAG CGCCTCCAAG AAGTTTCTAA GCTGACTAAA 
CAACTAATGC TCCTGGAGGC AAAAACCCAC GGCCAGGAAA AGGAGCTGGC GGAGAACAGG 
GAACAGCTGG AGATTTTACG TGCCAAATGC CAAGAACTCA AAACACACTC GGATGGCAAA 
ATCGCAGTGG AAGTTCATAA ATCAATTGTG AATGAATTAA AAAGCCAATT ACAGAAGGAA 
GAAGAGAAAG AAAGGGCTGA GATGGAGGAG TTGATGGAGA AGCTGACAGT CCTGCAAGCG 
CAGAAGAAGA GCCTGCTGTT AGAGAAGAAC AGTTTGACAG AGCAAAACAA AGCACTGGAA 
GCCGAACTTG AACGAGCACA GAAAATCAAT AGGAAATCTC AAAAGAAAAT TGAGGTCCTC 
AAAAAGCAGG TGGAAAAAGC CATGGGGAAC GAAATGTCTG CTCATCAGTA CCTGGCAAAC 
CTTGTTGGCC TGGCAGAAAA CATAACCCAG GAACGTGACA GTCTTATGTG TTTGGCAAAA 
TGTTTAGAAA GTGAGAAGGA TGGAGTGCTT AATAAAGTCA TAAAAAGCAA CATTCGCCTG 
GGAAAGTTAG AGGAAAAAGT CAAGGGCTAC AAGAAGCAGG CAGCACTGAA GCTGGGGGAC 
ATCAGTCACC GTCTGCTGGA GCAGCAGGAG GACTTCGCCG GCAAGACAGC CCAGTACCGG 
CAGGAGATGC GGCACCTGCA CCAGGTGCTG AAGGACAAGC AGGAGGTGCT GGACCAGGCG 
CTGCAGCAGA ACAGAGAAAT GGAAGGTGAA CTTGAAGTTA TTTGGGAATC TACCTTCAGG 
GAAAACCGAA GAATCCGAGA ACTTCTCCAG GACACACTCA CGAGGACAGG CGTGCAGGAC 
AACCCCAGAG CTCTGGTTGC CCCCAGCCTC AATGGCGTCT CTCAGGCAGA CCTGCTGGAC 
GGCTGCGATG TCTGCTCCTA TGACCTGAAG TCTCATGCCC CCACCTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011525727.1
CDS	344..1954
Translation	MDLNNMNQSLTLELNTMKQAMKELQLKLKGMEKEKRKLKEAEKASSQEVAAPELLYLRKQAQELVDENDGLKMTVHRLNVELSRYQTKFRHLSKEESLNIEGLPSKGPIPPWLLDIKYLSPLLLAYEDMMKEKDELNATLKEEMRMFRMRVQEVVKENEELHQELNKSSAVTSEEWRQLQTQAKLVLEENKLLLEQLEIQQRKAKDSHQERLQEVSKLTKQLMLLEAKTHGQEKELAENREQLEILRAKCQELKTHSDGKIAVEVHKSIVNELKSQLQKEEEKERAEMEELMEKLTVLQAQKKSLLLEKNSLTEQNKALEAELERAQKINRKSQKKIEVLKKQVEKAMGNEMSAHQYLANLVGLAENITQERDSLMCLAKCLESEKDGVLNKVIKSNIRLGKLEEKVKGYKKQAALKLGDISHRLLEQQEDFAGKTAQYRQEMRHLHQVLKDKQEVLDQALQQNREMEGELEVIWESTFRENRRIRELLQDTLTRTGVQDNPRALVAPSLNGVSQADLLDGCDVCSYDLKSHAPTC

Target ORF information:

RefSeq Version	XM_011527425.2
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 89 (CEP89), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527425.2

ORF Insert Sequence:

ATGGACCTAA ACAATATGAA TCAAAGCCTT ACCCTTGAAC TAAACACAAT GAAACAAGCA 
ATGAAAGAAC TACAGTTAAA ACTTAAGGGA ATGGAAAAAG AGAAGAGAAA GCTCAAAGAG 
GCTGAGAAGG CGTCGTCACA GGAAGTTGCT GCACCTGAAT TACTTTATCT GCGAAAACAA 
GCTCAAGAAC TGGTGGATGA AAATGATGGA TTGAAAATGA CTGTCCATCG TTTGAATGTA 
GAACTCAGTC GATATCAGAC AAAATTCAGG CATTTGTCCA AGGAAGAGAG CTTAAATATT 
GAAGGCCTCC CATCCAAGGG CCCTATACCA CCCTGGTTGT TGGATATAAA GTACCTGTCA 
CCATTGTTGC TGGCTTATGA AGATATGATG AAAGAGAAGG ACGAGCTCAA TGCCACCCTC 
AAGGAGGAAA TGAGAATGTT TAGGATGCGA GTCCAAGAAG TGGTGAAAGA AAATGAAGAA 
TTGCACCAAG AGTTAAATAA GAGTAGTGCT GTTACCAGTG AGGAATGGCG TCAGCTTCAG 
ACTCAAGCAA AACTGGTTTT AGAGGAAAAC AAGTTGTTGC TGGAGCAGTT GGAGATTCAG 
CAAAGGAAAG CCAAGGACAG CCACCAGGAG CGCCTCCAAG AAGTTTCTAA GCTGACTAAA 
CAACTAATGC TCCTGGAGGC AAAAACCCAC GGCCAGGAAA AGGAGCTGGC GGAGAACAGG 
GAACAGCTGG AGATTTTACG TGCCAAATGC CAAGAACTCA AAACACACTC GGATGGCAAA 
ATCGCAGTGG AAGTTCATAA ATCAATTGTG AATGAATTAA AAAGCCAATT ACAGAAGGAA 
GAAGAGAAAG AAAGGGCTGA GATGGAGGAG TTGATGGAGA AGCTGACAGT CCTGCAAGCG 
CAGAAGAAGA GCCTGCTGTT AGAGAAGAAC AGTTTGACAG AGCAAAACAA AGCACTGGAA 
GCCGAACTTG AACGAGCACA GAAAATCAAT AGGAAATCTC AAAAGAAAAT TGAGGTCCTC 
AAAAAGCAGG TGGAAAAAGC CATGGGGAAC GAAATGTCTG CTCATCAGTA CCTGGCAAAC 
CTTGTTGGCC TGGCAGAAAA CATAACCCAG GAACGTGACA GTCTTATGTG TTTGGCAAAA 
TGTTTAGAAA GTGAGAAGGA TGGAGTGCTT AATAAAGTCA TAAAAAGCAA CATTCGCCTG 
GGAAAGTTAG AGGAAAAAGT CAAGGGCTAC AAGAAGCAGG CAGCACTGAA GCTGGGGGAC 
ATCAGTCACC GTCTGCTGGA GCAGCAGGAG GACTTCGCCG GCAAGACAGC CCAGTACCGG 
CAGGAGATGC GGCACCTGCA CCAGGTGCTG AAGGACAAGC AGGAGGTGCT GGACCAGGCG 
CTGCAGCAGA ACAGAGAAAT GGAAGGTGAA CTTGAAGTTA TTTGGGAATC TACCTTCAGG 
GAAAACCGAA GAATCCGAGA ACTTCTCCAG GACACACTCA CGAGGACAGG CGTGCAGGAC 
AACCCCAGAG CTCTGGTTGC CCCCAGCCTC AATGGCGTCT CTCAGGCAGA CCTGCTGGAC 
GGCTGCGATG TCTGCTCCTA TGACCTGAAG TCTCATGCCC CCACCTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP89 ( XM_024451745.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024307513.1 Homo sapiens centrosomal protein 89 (CEP89), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu52551
Clone ID Related Accession (Same CDS sequence)	XM_011527425.2 , XM_024451745.1
Accession Version	XM_024451745.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	centrosomal protein of 89 kDa isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTAA ACAATATGAA TCAAAGCCTT ACCCTTGAAC TAAACACAAT GAAACAAGCA 
ATGAAAGAAC TACAGTTAAA ACTTAAGGGA ATGGAAAAAG AGAAGAGAAA GCTCAAAGAG 
GCTGAGAAGG CGTCGTCACA GGAAGTTGCT GCACCTGAAT TACTTTATCT GCGAAAACAA 
GCTCAAGAAC TGGTGGATGA AAATGATGGA TTGAAAATGA CTGTCCATCG TTTGAATGTA 
GAACTCAGTC GATATCAGAC AAAATTCAGG CATTTGTCCA AGGAAGAGAG CTTAAATATT 
GAAGGCCTCC CATCCAAGGG CCCTATACCA CCCTGGTTGT TGGATATAAA GTACCTGTCA 
CCATTGTTGC TGGCTTATGA AGATATGATG AAAGAGAAGG ACGAGCTCAA TGCCACCCTC 
AAGGAGGAAA TGAGAATGTT TAGGATGCGA GTCCAAGAAG TGGTGAAAGA AAATGAAGAA 
TTGCACCAAG AGTTAAATAA GAGTAGTGCT GTTACCAGTG AGGAATGGCG TCAGCTTCAG 
ACTCAAGCAA AACTGGTTTT AGAGGAAAAC AAGTTGTTGC TGGAGCAGTT GGAGATTCAG 
CAAAGGAAAG CCAAGGACAG CCACCAGGAG CGCCTCCAAG AAGTTTCTAA GCTGACTAAA 
CAACTAATGC TCCTGGAGGC AAAAACCCAC GGCCAGGAAA AGGAGCTGGC GGAGAACAGG 
GAACAGCTGG AGATTTTACG TGCCAAATGC CAAGAACTCA AAACACACTC GGATGGCAAA 
ATCGCAGTGG AAGTTCATAA ATCAATTGTG AATGAATTAA AAAGCCAATT ACAGAAGGAA 
GAAGAGAAAG AAAGGGCTGA GATGGAGGAG TTGATGGAGA AGCTGACAGT CCTGCAAGCG 
CAGAAGAAGA GCCTGCTGTT AGAGAAGAAC AGTTTGACAG AGCAAAACAA AGCACTGGAA 
GCCGAACTTG AACGAGCACA GAAAATCAAT AGGAAATCTC AAAAGAAAAT TGAGGTCCTC 
AAAAAGCAGG TGGAAAAAGC CATGGGGAAC GAAATGTCTG CTCATCAGTA CCTGGCAAAC 
CTTGTTGGCC TGGCAGAAAA CATAACCCAG GAACGTGACA GTCTTATGTG TTTGGCAAAA 
TGTTTAGAAA GTGAGAAGGA TGGAGTGCTT AATAAAGTCA TAAAAAGCAA CATTCGCCTG 
GGAAAGTTAG AGGAAAAAGT CAAGGGCTAC AAGAAGCAGG CAGCACTGAA GCTGGGGGAC 
ATCAGTCACC GTCTGCTGGA GCAGCAGGAG GACTTCGCCG GCAAGACAGC CCAGTACCGG 
CAGGAGATGC GGCACCTGCA CCAGGTGCTG AAGGACAAGC AGGAGGTGCT GGACCAGGCG 
CTGCAGCAGA ACAGAGAAAT GGAAGGTGAA CTTGAAGTTA TTTGGGAATC TACCTTCAGG 
GAAAACCGAA GAATCCGAGA ACTTCTCCAG GACACACTCA CGAGGACAGG CGTGCAGGAC 
AACCCCAGAG CTCTGGTTGC CCCCAGCCTC AATGGCGTCT CTCAGGCAGA CCTGCTGGAC 
GGCTGCGATG TCTGCTCCTA TGACCTGAAG TCTCATGCCC CCACCTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024307513.1
CDS	962..2572
Translation	MDLNNMNQSLTLELNTMKQAMKELQLKLKGMEKEKRKLKEAEKASSQEVAAPELLYLRKQAQELVDENDGLKMTVHRLNVELSRYQTKFRHLSKEESLNIEGLPSKGPIPPWLLDIKYLSPLLLAYEDMMKEKDELNATLKEEMRMFRMRVQEVVKENEELHQELNKSSAVTSEEWRQLQTQAKLVLEENKLLLEQLEIQQRKAKDSHQERLQEVSKLTKQLMLLEAKTHGQEKELAENREQLEILRAKCQELKTHSDGKIAVEVHKSIVNELKSQLQKEEEKERAEMEELMEKLTVLQAQKKSLLLEKNSLTEQNKALEAELERAQKINRKSQKKIEVLKKQVEKAMGNEMSAHQYLANLVGLAENITQERDSLMCLAKCLESEKDGVLNKVIKSNIRLGKLEEKVKGYKKQAALKLGDISHRLLEQQEDFAGKTAQYRQEMRHLHQVLKDKQEVLDQALQQNREMEGELEVIWESTFRENRRIRELLQDTLTRTGVQDNPRALVAPSLNGVSQADLLDGCDVCSYDLKSHAPTC

Target ORF information:

RefSeq Version	XM_024451745.1
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 89 (CEP89), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451745.1

ORF Insert Sequence:

ATGGACCTAA ACAATATGAA TCAAAGCCTT ACCCTTGAAC TAAACACAAT GAAACAAGCA 
ATGAAAGAAC TACAGTTAAA ACTTAAGGGA ATGGAAAAAG AGAAGAGAAA GCTCAAAGAG 
GCTGAGAAGG CGTCGTCACA GGAAGTTGCT GCACCTGAAT TACTTTATCT GCGAAAACAA 
GCTCAAGAAC TGGTGGATGA AAATGATGGA TTGAAAATGA CTGTCCATCG TTTGAATGTA 
GAACTCAGTC GATATCAGAC AAAATTCAGG CATTTGTCCA AGGAAGAGAG CTTAAATATT 
GAAGGCCTCC CATCCAAGGG CCCTATACCA CCCTGGTTGT TGGATATAAA GTACCTGTCA 
CCATTGTTGC TGGCTTATGA AGATATGATG AAAGAGAAGG ACGAGCTCAA TGCCACCCTC 
AAGGAGGAAA TGAGAATGTT TAGGATGCGA GTCCAAGAAG TGGTGAAAGA AAATGAAGAA 
TTGCACCAAG AGTTAAATAA GAGTAGTGCT GTTACCAGTG AGGAATGGCG TCAGCTTCAG 
ACTCAAGCAA AACTGGTTTT AGAGGAAAAC AAGTTGTTGC TGGAGCAGTT GGAGATTCAG 
CAAAGGAAAG CCAAGGACAG CCACCAGGAG CGCCTCCAAG AAGTTTCTAA GCTGACTAAA 
CAACTAATGC TCCTGGAGGC AAAAACCCAC GGCCAGGAAA AGGAGCTGGC GGAGAACAGG 
GAACAGCTGG AGATTTTACG TGCCAAATGC CAAGAACTCA AAACACACTC GGATGGCAAA 
ATCGCAGTGG AAGTTCATAA ATCAATTGTG AATGAATTAA AAAGCCAATT ACAGAAGGAA 
GAAGAGAAAG AAAGGGCTGA GATGGAGGAG TTGATGGAGA AGCTGACAGT CCTGCAAGCG 
CAGAAGAAGA GCCTGCTGTT AGAGAAGAAC AGTTTGACAG AGCAAAACAA AGCACTGGAA 
GCCGAACTTG AACGAGCACA GAAAATCAAT AGGAAATCTC AAAAGAAAAT TGAGGTCCTC 
AAAAAGCAGG TGGAAAAAGC CATGGGGAAC GAAATGTCTG CTCATCAGTA CCTGGCAAAC 
CTTGTTGGCC TGGCAGAAAA CATAACCCAG GAACGTGACA GTCTTATGTG TTTGGCAAAA 
TGTTTAGAAA GTGAGAAGGA TGGAGTGCTT AATAAAGTCA TAAAAAGCAA CATTCGCCTG 
GGAAAGTTAG AGGAAAAAGT CAAGGGCTAC AAGAAGCAGG CAGCACTGAA GCTGGGGGAC 
ATCAGTCACC GTCTGCTGGA GCAGCAGGAG GACTTCGCCG GCAAGACAGC CCAGTACCGG 
CAGGAGATGC GGCACCTGCA CCAGGTGCTG AAGGACAAGC AGGAGGTGCT GGACCAGGCG 
CTGCAGCAGA ACAGAGAAAT GGAAGGTGAA CTTGAAGTTA TTTGGGAATC TACCTTCAGG 
GAAAACCGAA GAATCCGAGA ACTTCTCCAG GACACACTCA CGAGGACAGG CGTGCAGGAC 
AACCCCAGAG CTCTGGTTGC CCCCAGCCTC AATGGCGTCT CTCAGGCAGA CCTGCTGGAC 
GGCTGCGATG TCTGCTCCTA TGACCTGAAG TCTCATGCCC CCACCTGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP89 ( NM_032816.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_116205.3 Homo sapiens centrosomal protein 89 (CEP89), mRNA.

Price & Availability↑

CloneID	OHu73828
Clone ID Related Accession (Same CDS sequence)	NM_032816.4 , NM_032816.5
Accession Version	NM_032816.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	centrosomal protein of 89 kDa
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA040903.1, BC136328.1, BU196203.1, DB539713.2 and BU628988.1. On Dec 14, 2014 this sequence version replaced NM_032816.3. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.112968.1, SRR1803617.77499.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTCCTGG GATTTCGGAG AGGCCGCAGG AGTCATTTCA AACACATCAT CCATGGCCTT 
TTACCTGCAG CCAGCGTTGC TCCGAAGGCA GCTGTGCCAC GCACACCTCC TCCCCGCAGC 
CCCAACCCAT CTCCAGAGAG ACCAAGATCT GCTCTGGCAG CAGCCATTCT GGCGACAACA 
TTGACTGGGC GGACGGTTGC TATTCCTCAG CCTCGCCAGA GGTCCCGGTC TGAGAGTGAT 
GTGAGCAGTG TTGAACAGGA CAGCTTCATC GAGCCCTATG CCACCACCTC ACAGCTGAGG 
CCTCGGCCAA ATTGGCAGAG TGAGATGGGA AGAAGATCTT CTTTGCCATC CTTTGAAACA 
CTGGACTATG GGGACGAAGA GGACATTGAA ACTCAGCTGT CATCCAGCGG CAAGGAATTG 
GGGGATGTCA GTGCCCGGGA GGACAGAGGA GGCCACAGTG ATGACCTGTA CGCTGTGCCA 
CACAGAAATC AGGTGCCATT GTTACATGAG GTGAACAGTG AAGACGATGA AAATATTTCT 
CATCAAGATG GGTTTCCAGG CTCCCCTCCT GCACCACAGC GGACACAACA AAAAGATGGT 
AAACACCCTG TTCTGAATTT AAAGGATGAA AAACCTCCAT TATGTGAGAA ACCTCCACCC 
TCCCCAGATA TAACTGGTAG AGCACGTCAA AGATATACAG AAATAACCAG AGAAAAGTTT 
GAGGCATTAA AAGAAGAAAA TATGGACCTA AACAATATGA ATCAAAGCCT TACCCTTGAA 
CTAAACACAA TGAAACAAGC AATGAAAGAA CTACAGTTAA AACTTAAGGG AATGGAAAAA 
GAGAAGAGAA AGCTCAAAGA GGCTGAGAAG GCGTCGTCAC AGGAAGTTGC TGCACCTGAA 
TTACTTTATC TGCGAAAACA AGCTCAAGAA CTGGTGGATG AAAATGATGG ATTGAAAATG 
ACTGTCCATC GTTTGAATGT AGAACTCAGT CGATATCAGA CAAAATTCAG GCATTTGTCC 
AAGGAAGAGA GCTTAAATAT TGAAGGCCTC CCATCCAAGG GCCCTATACC ACCCTGGTTG 
TTGGATATAA AGTACCTGTC ACCATTGTTG CTGGCTTATG AAGATATGAT GAAAGAGAAG 
GACGAGCTCA ATGCCACCCT CAAGGAGGAA ATGAGAATGT TTAGGATGCG AGTCCAAGAA 
GTGGTGAAAG AAAATGAAGA ATTGCACCAA GAGTTAAATA AGAGTAGTGC TGTTACCAGT 
GAGGAATGGC GTCAGCTTCA GACTCAAGCA AAACTGGTTT TAGAGGAAAA CAAGTTGTTG 
CTGGAGCAGT TGGAGATTCA GCAAAGGAAA GCCAAGGACA GCCACCAGGA GCGCCTCCAA 
GAAGTTTCTA AGCTGACTAA ACAACTAATG CTCCTGGAGG CAAAAACCCA CGGCCAGGAA 
AAGGAGCTGG CGGAGAACAG GGAACAGCTG GAGATTTTAC GTGCCAAATG CCAAGAACTC 
AAAACACACT CGGATGGCAA AATCGCAGTG GAAGTTCATA AATCAATTGT GAATGAATTA 
AAAAGCCAAT TACAGAAGGA AGAAGAGAAA GAAAGGGCTG AGATGGAGGA GTTGATGGAG 
AAGCTGACAG TCCTGCAAGC GCAGAAGAAG AGCCTGCTGT TAGAGAAGAA CAGTTTGACA 
GAGCAAAACA AAGCACTGGA AGCCGAACTT GAACGAGCAC AGAAAATCAA TAGGAAATCT 
CAAAAGAAAA TTGAGGTCCT CAAAAAGCAG GTGGAAAAAG CCATGGGGAA CGAAATGTCT 
GCTCATCAGT ACCTGGCAAA CCTTGTTGGC CTGGCAGAAA ACATAACCCA GGAACGTGAC 
AGTCTTATGT GTTTGGCAAA ATGTTTAGAA AGTGAGAAGG ATGGAGTGCT TAATAAAGTC 
ATAAAAAGCA ACATTCGCCT GGGAAAGTTA GAGGAAAAAG TCAAGGGCTA CAAGAAGCAG 
GCAGCACTGA AGCTGGGGGA CATCAGTCAC CGTCTGCTGG AGCAGCAGGA GGACTTCGCC 
GGCAAGACAG CCCAGTACCG GCAGGAGATG CGGCACCTGC ACCAGGTGCT GAAGGACAAG 
CAGGAGGTGC TGGACCAGGC GCTGCAGCAG AACAGAGAAA TGGAAGGTGA ACTTGAAGTT 
ATTTGGGAAT CTACCTTCAG GGAAAACCGA AGAATCCGAG AACTTCTCCA GGACACACTC 
ACGAGGACAG GCGTGCAGGA CAACCCCAGA GCTCTGGTTG CCCCCAGCCT CAATGGCGTC 
TCTCAGGCAG ACCTGCTGGA CGGCTGCGAT GTCTGCTCCT ATGACCTGAA GTCTCATGCC 
CCCACCTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116205.3
CDS	156..2507
Translation	MLLGFRRGRRSHFKHIIHGLLPAASVAPKAAVPRTPPPRSPNPSPERPRSALAAAILATTLTGRTVAIPQPRQRSRSESDVSSVEQDSFIEPYATTSQLRPRPNWQSEMGRRSSLPSFETLDYGDEEDIETQLSSSGKELGDVSAREDRGGHSDDLYAVPHRNQVPLLHEVNSEDDENISHQDGFPGSPPAPQRTQQKDGKHPVLNLKDEKPPLCEKPPPSPDITGRARQRYTEITREKFEALKEENMDLNNMNQSLTLELNTMKQAMKELQLKLKGMEKEKRKLKEAEKASSQEVAAPELLYLRKQAQELVDENDGLKMTVHRLNVELSRYQTKFRHLSKEESLNIEGLPSKGPIPPWLLDIKYLSPLLLAYEDMMKEKDELNATLKEEMRMFRMRVQEVVKENEELHQELNKSSAVTSEEWRQLQTQAKLVLEENKLLLEQLEIQQRKAKDSHQERLQEVSKLTKQLMLLEAKTHGQEKELAENREQLEILRAKCQELKTHSDGKIAVEVHKSIVNELKSQLQKEEEKERAEMEELMEKLTVLQAQKKSLLLEKNSLTEQNKALEAELERAQKINRKSQKKIEVLKKQVEKAMGNEMSAHQYLANLVGLAENITQERDSLMCLAKCLESEKDGVLNKVIKSNIRLGKLEEKVKGYKKQAALKLGDISHRLLEQQEDFAGKTAQYRQEMRHLHQVLKDKQEVLDQALQQNREMEGELEVIWESTFRENRRIRELLQDTLTRTGVQDNPRALVAPSLNGVSQADLLDGCDVCSYDLKSHAPTC

Target ORF information:

RefSeq Version	NM_032816.4
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 89 (CEP89), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032816.4

ORF Insert Sequence:

ATGCTCCTGG GATTTCGGAG AGGCCGCAGG AGTCATTTCA AACACATCAT CCATGGCCTT 
TTACCTGCAG CCAGCGTTGC TCCGAAGGCA GCTGTGCCAC GCACACCTCC TCCCCGCAGC 
CCCAACCCAT CTCCAGAGAG ACCAAGATCT GCTCTGGCAG CAGCCATTCT GGCGACAACA 
TTGACTGGGC GGACGGTTGC TATTCCTCAG CCTCGCCAGA GGTCCCGGTC TGAGAGTGAT 
GTGAGCAGTG TTGAACAGGA CAGCTTCATC GAGCCCTATG CCACCACCTC ACAGCTGAGG 
CCTCGGCCAA ATTGGCAGAG TGAGATGGGA AGAAGATCTT CTTTGCCATC CTTTGAAACA 
CTGGACTATG GGGACGAAGA GGACATTGAA ACTCAGCTGT CATCCAGCGG CAAGGAATTG 
GGGGATGTCA GTGCCCGGGA GGACAGAGGA GGCCACAGTG ATGACCTGTA CGCTGTGCCA 
CACAGAAATC AGGTGCCATT GTTACATGAG GTGAACAGTG AAGACGATGA AAATATTTCT 
CATCAAGATG GGTTTCCAGG CTCCCCTCCT GCACCACAGC GGACACAACA AAAAGATGGT 
AAACACCCTG TTCTGAATTT AAAGGATGAA AAACCTCCAT TATGTGAGAA ACCTCCACCC 
TCCCCAGATA TAACTGGTAG AGCACGTCAA AGATATACAG AAATAACCAG AGAAAAGTTT 
GAGGCATTAA AAGAAGAAAA TATGGACCTA AACAATATGA ATCAAAGCCT TACCCTTGAA 
CTAAACACAA TGAAACAAGC AATGAAAGAA CTACAGTTAA AACTTAAGGG AATGGAAAAA 
GAGAAGAGAA AGCTCAAAGA GGCTGAGAAG GCGTCGTCAC AGGAAGTTGC TGCACCTGAA 
TTACTTTATC TGCGAAAACA AGCTCAAGAA CTGGTGGATG AAAATGATGG ATTGAAAATG 
ACTGTCCATC GTTTGAATGT AGAACTCAGT CGATATCAGA CAAAATTCAG GCATTTGTCC 
AAGGAAGAGA GCTTAAATAT TGAAGGCCTC CCATCCAAGG GCCCTATACC ACCCTGGTTG 
TTGGATATAA AGTACCTGTC ACCATTGTTG CTGGCTTATG AAGATATGAT GAAAGAGAAG 
GACGAGCTCA ATGCCACCCT CAAGGAGGAA ATGAGAATGT TTAGGATGCG AGTCCAAGAA 
GTGGTGAAAG AAAATGAAGA ATTGCACCAA GAGTTAAATA AGAGTAGTGC TGTTACCAGT 
GAGGAATGGC GTCAGCTTCA GACTCAAGCA AAACTGGTTT TAGAGGAAAA CAAGTTGTTG 
CTGGAGCAGT TGGAGATTCA GCAAAGGAAA GCCAAGGACA GCCACCAGGA GCGCCTCCAA 
GAAGTTTCTA AGCTGACTAA ACAACTAATG CTCCTGGAGG CAAAAACCCA CGGCCAGGAA 
AAGGAGCTGG CGGAGAACAG GGAACAGCTG GAGATTTTAC GTGCCAAATG CCAAGAACTC 
AAAACACACT CGGATGGCAA AATCGCAGTG GAAGTTCATA AATCAATTGT GAATGAATTA 
AAAAGCCAAT TACAGAAGGA AGAAGAGAAA GAAAGGGCTG AGATGGAGGA GTTGATGGAG 
AAGCTGACAG TCCTGCAAGC GCAGAAGAAG AGCCTGCTGT TAGAGAAGAA CAGTTTGACA 
GAGCAAAACA AAGCACTGGA AGCCGAACTT GAACGAGCAC AGAAAATCAA TAGGAAATCT 
CAAAAGAAAA TTGAGGTCCT CAAAAAGCAG GTGGAAAAAG CCATGGGGAA CGAAATGTCT 
GCTCATCAGT ACCTGGCAAA CCTTGTTGGC CTGGCAGAAA ACATAACCCA GGAACGTGAC 
AGTCTTATGT GTTTGGCAAA ATGTTTAGAA AGTGAGAAGG ATGGAGTGCT TAATAAAGTC 
ATAAAAAGCA ACATTCGCCT GGGAAAGTTA GAGGAAAAAG TCAAGGGCTA CAAGAAGCAG 
GCAGCACTGA AGCTGGGGGA CATCAGTCAC CGTCTGCTGG AGCAGCAGGA GGACTTCGCC 
GGCAAGACAG CCCAGTACCG GCAGGAGATG CGGCACCTGC ACCAGGTGCT GAAGGACAAG 
CAGGAGGTGC TGGACCAGGC GCTGCAGCAG AACAGAGAAA TGGAAGGTGA ACTTGAAGTT 
ATTTGGGAAT CTACCTTCAG GGAAAACCGA AGAATCCGAG AACTTCTCCA GGACACACTC 
ACGAGGACAG GCGTGCAGGA CAACCCCAGA GCTCTGGTTG CCCCCAGCCT CAATGGCGTC 
TCTCAGGCAG ACCTGCTGGA CGGCTGCGAT GTCTGCTCCT ATGACCTGAA GTCTCATGCC 
CCCACCTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP89 ( NM_032816.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_116205.3 Homo sapiens centrosomal protein 89 (CEP89), mRNA.

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CloneID	OHu73828
Clone ID Related Accession (Same CDS sequence)	NM_032816.4 , NM_032816.5
Accession Version	NM_032816.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	centrosomal protein of 89 kDa
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA040903.1, BC136328.1, BU196203.1, DB539713.2, BU628988.1 and AC008805.8. On Nov 23, 2018 this sequence version replaced NM_032816.4. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.112968.1, SRR1803615.232532.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000305768.10/ ENSP00000306105.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTCCTGG GATTTCGGAG AGGCCGCAGG AGTCATTTCA AACACATCAT CCATGGCCTT 
TTACCTGCAG CCAGCGTTGC TCCGAAGGCA GCTGTGCCAC GCACACCTCC TCCCCGCAGC 
CCCAACCCAT CTCCAGAGAG ACCAAGATCT GCTCTGGCAG CAGCCATTCT GGCGACAACA 
TTGACTGGGC GGACGGTTGC TATTCCTCAG CCTCGCCAGA GGTCCCGGTC TGAGAGTGAT 
GTGAGCAGTG TTGAACAGGA CAGCTTCATC GAGCCCTATG CCACCACCTC ACAGCTGAGG 
CCTCGGCCAA ATTGGCAGAG TGAGATGGGA AGAAGATCTT CTTTGCCATC CTTTGAAACA 
CTGGACTATG GGGACGAAGA GGACATTGAA ACTCAGCTGT CATCCAGCGG CAAGGAATTG 
GGGGATGTCA GTGCCCGGGA GGACAGAGGA GGCCACAGTG ATGACCTGTA CGCTGTGCCA 
CACAGAAATC AGGTGCCATT GTTACATGAG GTGAACAGTG AAGACGATGA AAATATTTCT 
CATCAAGATG GGTTTCCAGG CTCCCCTCCT GCACCACAGC GGACACAACA AAAAGATGGT 
AAACACCCTG TTCTGAATTT AAAGGATGAA AAACCTCCAT TATGTGAGAA ACCTCCACCC 
TCCCCAGATA TAACTGGTAG AGCACGTCAA AGATATACAG AAATAACCAG AGAAAAGTTT 
GAGGCATTAA AAGAAGAAAA TATGGACCTA AACAATATGA ATCAAAGCCT TACCCTTGAA 
CTAAACACAA TGAAACAAGC AATGAAAGAA CTACAGTTAA AACTTAAGGG AATGGAAAAA 
GAGAAGAGAA AGCTCAAAGA GGCTGAGAAG GCGTCGTCAC AGGAAGTTGC TGCACCTGAA 
TTACTTTATC TGCGAAAACA AGCTCAAGAA CTGGTGGATG AAAATGATGG ATTGAAAATG 
ACTGTCCATC GTTTGAATGT AGAACTCAGT CGATATCAGA CAAAATTCAG GCATTTGTCC 
AAGGAAGAGA GCTTAAATAT TGAAGGCCTC CCATCCAAGG GCCCTATACC ACCCTGGTTG 
TTGGATATAA AGTACCTGTC ACCATTGTTG CTGGCTTATG AAGATATGAT GAAAGAGAAG 
GACGAGCTCA ATGCCACCCT CAAGGAGGAA ATGAGAATGT TTAGGATGCG AGTCCAAGAA 
GTGGTGAAAG AAAATGAAGA ATTGCACCAA GAGTTAAATA AGAGTAGTGC TGTTACCAGT 
GAGGAATGGC GTCAGCTTCA GACTCAAGCA AAACTGGTTT TAGAGGAAAA CAAGTTGTTG 
CTGGAGCAGT TGGAGATTCA GCAAAGGAAA GCCAAGGACA GCCACCAGGA GCGCCTCCAA 
GAAGTTTCTA AGCTGACTAA ACAACTAATG CTCCTGGAGG CAAAAACCCA CGGCCAGGAA 
AAGGAGCTGG CGGAGAACAG GGAACAGCTG GAGATTTTAC GTGCCAAATG CCAAGAACTC 
AAAACACACT CGGATGGCAA AATCGCAGTG GAAGTTCATA AATCAATTGT GAATGAATTA 
AAAAGCCAAT TACAGAAGGA AGAAGAGAAA GAAAGGGCTG AGATGGAGGA GTTGATGGAG 
AAGCTGACAG TCCTGCAAGC GCAGAAGAAG AGCCTGCTGT TAGAGAAGAA CAGTTTGACA 
GAGCAAAACA AAGCACTGGA AGCCGAACTT GAACGAGCAC AGAAAATCAA TAGGAAATCT 
CAAAAGAAAA TTGAGGTCCT CAAAAAGCAG GTGGAAAAAG CCATGGGGAA CGAAATGTCT 
GCTCATCAGT ACCTGGCAAA CCTTGTTGGC CTGGCAGAAA ACATAACCCA GGAACGTGAC 
AGTCTTATGT GTTTGGCAAA ATGTTTAGAA AGTGAGAAGG ATGGAGTGCT TAATAAAGTC 
ATAAAAAGCA ACATTCGCCT GGGAAAGTTA GAGGAAAAAG TCAAGGGCTA CAAGAAGCAG 
GCAGCACTGA AGCTGGGGGA CATCAGTCAC CGTCTGCTGG AGCAGCAGGA GGACTTCGCC 
GGCAAGACAG CCCAGTACCG GCAGGAGATG CGGCACCTGC ACCAGGTGCT GAAGGACAAG 
CAGGAGGTGC TGGACCAGGC GCTGCAGCAG AACAGAGAAA TGGAAGGTGA ACTTGAAGTT 
ATTTGGGAAT CTACCTTCAG GGAAAACCGA AGAATCCGAG AACTTCTCCA GGACACACTC 
ACGAGGACAG GCGTGCAGGA CAACCCCAGA GCTCTGGTTG CCCCCAGCCT CAATGGCGTC 
TCTCAGGCAG ACCTGCTGGA CGGCTGCGAT GTCTGCTCCT ATGACCTGAA GTCTCATGCC 
CCCACCTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116205.3
CDS	85..2436
Translation	MLLGFRRGRRSHFKHIIHGLLPAASVAPKAAVPRTPPPRSPNPSPERPRSALAAAILATTLTGRTVAIPQPRQRSRSESDVSSVEQDSFIEPYATTSQLRPRPNWQSEMGRRSSLPSFETLDYGDEEDIETQLSSSGKELGDVSAREDRGGHSDDLYAVPHRNQVPLLHEVNSEDDENISHQDGFPGSPPAPQRTQQKDGKHPVLNLKDEKPPLCEKPPPSPDITGRARQRYTEITREKFEALKEENMDLNNMNQSLTLELNTMKQAMKELQLKLKGMEKEKRKLKEAEKASSQEVAAPELLYLRKQAQELVDENDGLKMTVHRLNVELSRYQTKFRHLSKEESLNIEGLPSKGPIPPWLLDIKYLSPLLLAYEDMMKEKDELNATLKEEMRMFRMRVQEVVKENEELHQELNKSSAVTSEEWRQLQTQAKLVLEENKLLLEQLEIQQRKAKDSHQERLQEVSKLTKQLMLLEAKTHGQEKELAENREQLEILRAKCQELKTHSDGKIAVEVHKSIVNELKSQLQKEEEKERAEMEELMEKLTVLQAQKKSLLLEKNSLTEQNKALEAELERAQKINRKSQKKIEVLKKQVEKAMGNEMSAHQYLANLVGLAENITQERDSLMCLAKCLESEKDGVLNKVIKSNIRLGKLEEKVKGYKKQAALKLGDISHRLLEQQEDFAGKTAQYRQEMRHLHQVLKDKQEVLDQALQQNREMEGELEVIWESTFRENRRIRELLQDTLTRTGVQDNPRALVAPSLNGVSQADLLDGCDVCSYDLKSHAPTC

Target ORF information:

RefSeq Version	NM_032816.5
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 89 (CEP89), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032816.5

ORF Insert Sequence:

ATGCTCCTGG GATTTCGGAG AGGCCGCAGG AGTCATTTCA AACACATCAT CCATGGCCTT 
TTACCTGCAG CCAGCGTTGC TCCGAAGGCA GCTGTGCCAC GCACACCTCC TCCCCGCAGC 
CCCAACCCAT CTCCAGAGAG ACCAAGATCT GCTCTGGCAG CAGCCATTCT GGCGACAACA 
TTGACTGGGC GGACGGTTGC TATTCCTCAG CCTCGCCAGA GGTCCCGGTC TGAGAGTGAT 
GTGAGCAGTG TTGAACAGGA CAGCTTCATC GAGCCCTATG CCACCACCTC ACAGCTGAGG 
CCTCGGCCAA ATTGGCAGAG TGAGATGGGA AGAAGATCTT CTTTGCCATC CTTTGAAACA 
CTGGACTATG GGGACGAAGA GGACATTGAA ACTCAGCTGT CATCCAGCGG CAAGGAATTG 
GGGGATGTCA GTGCCCGGGA GGACAGAGGA GGCCACAGTG ATGACCTGTA CGCTGTGCCA 
CACAGAAATC AGGTGCCATT GTTACATGAG GTGAACAGTG AAGACGATGA AAATATTTCT 
CATCAAGATG GGTTTCCAGG CTCCCCTCCT GCACCACAGC GGACACAACA AAAAGATGGT 
AAACACCCTG TTCTGAATTT AAAGGATGAA AAACCTCCAT TATGTGAGAA ACCTCCACCC 
TCCCCAGATA TAACTGGTAG AGCACGTCAA AGATATACAG AAATAACCAG AGAAAAGTTT 
GAGGCATTAA AAGAAGAAAA TATGGACCTA AACAATATGA ATCAAAGCCT TACCCTTGAA 
CTAAACACAA TGAAACAAGC AATGAAAGAA CTACAGTTAA AACTTAAGGG AATGGAAAAA 
GAGAAGAGAA AGCTCAAAGA GGCTGAGAAG GCGTCGTCAC AGGAAGTTGC TGCACCTGAA 
TTACTTTATC TGCGAAAACA AGCTCAAGAA CTGGTGGATG AAAATGATGG ATTGAAAATG 
ACTGTCCATC GTTTGAATGT AGAACTCAGT CGATATCAGA CAAAATTCAG GCATTTGTCC 
AAGGAAGAGA GCTTAAATAT TGAAGGCCTC CCATCCAAGG GCCCTATACC ACCCTGGTTG 
TTGGATATAA AGTACCTGTC ACCATTGTTG CTGGCTTATG AAGATATGAT GAAAGAGAAG 
GACGAGCTCA ATGCCACCCT CAAGGAGGAA ATGAGAATGT TTAGGATGCG AGTCCAAGAA 
GTGGTGAAAG AAAATGAAGA ATTGCACCAA GAGTTAAATA AGAGTAGTGC TGTTACCAGT 
GAGGAATGGC GTCAGCTTCA GACTCAAGCA AAACTGGTTT TAGAGGAAAA CAAGTTGTTG 
CTGGAGCAGT TGGAGATTCA GCAAAGGAAA GCCAAGGACA GCCACCAGGA GCGCCTCCAA 
GAAGTTTCTA AGCTGACTAA ACAACTAATG CTCCTGGAGG CAAAAACCCA CGGCCAGGAA 
AAGGAGCTGG CGGAGAACAG GGAACAGCTG GAGATTTTAC GTGCCAAATG CCAAGAACTC 
AAAACACACT CGGATGGCAA AATCGCAGTG GAAGTTCATA AATCAATTGT GAATGAATTA 
AAAAGCCAAT TACAGAAGGA AGAAGAGAAA GAAAGGGCTG AGATGGAGGA GTTGATGGAG 
AAGCTGACAG TCCTGCAAGC GCAGAAGAAG AGCCTGCTGT TAGAGAAGAA CAGTTTGACA 
GAGCAAAACA AAGCACTGGA AGCCGAACTT GAACGAGCAC AGAAAATCAA TAGGAAATCT 
CAAAAGAAAA TTGAGGTCCT CAAAAAGCAG GTGGAAAAAG CCATGGGGAA CGAAATGTCT 
GCTCATCAGT ACCTGGCAAA CCTTGTTGGC CTGGCAGAAA ACATAACCCA GGAACGTGAC 
AGTCTTATGT GTTTGGCAAA ATGTTTAGAA AGTGAGAAGG ATGGAGTGCT TAATAAAGTC 
ATAAAAAGCA ACATTCGCCT GGGAAAGTTA GAGGAAAAAG TCAAGGGCTA CAAGAAGCAG 
GCAGCACTGA AGCTGGGGGA CATCAGTCAC CGTCTGCTGG AGCAGCAGGA GGACTTCGCC 
GGCAAGACAG CCCAGTACCG GCAGGAGATG CGGCACCTGC ACCAGGTGCT GAAGGACAAG 
CAGGAGGTGC TGGACCAGGC GCTGCAGCAG AACAGAGAAA TGGAAGGTGA ACTTGAAGTT 
ATTTGGGAAT CTACCTTCAG GGAAAACCGA AGAATCCGAG AACTTCTCCA GGACACACTC 
ACGAGGACAG GCGTGCAGGA CAACCCCAGA GCTCTGGTTG CCCCCAGCCT CAATGGCGTC 
TCTCAGGCAG ACCTGCTGGA CGGCTGCGAT GTCTGCTCCT ATGACCTGAA GTCTCATGCC 
CCCACCTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP89 ( XM_005259344.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005259401.1 Homo sapiens centrosomal protein 89 (CEP89), transcript variant X1, mRNA.

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CloneID	OHu52550
Clone ID Related Accession (Same CDS sequence)	XM_005259344.3
Accession Version	XM_005259344.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	centrosomal protein of 89 kDa isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005259344.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTGG GATTTCGGAG AGGCCGCAGG AGTCATTTCA AACACATCAT CCATGGCCTT 
TTACCTGCAG CCAGCGTTGC TCCGAAGGCA GCTGTGCCAC GCACACCTCC TCCCCGCAGC 
CCCAACCCAT CTCCAGAGAG ACCAAGATCT GCTCTGGCAG CAGCCATTCT GGCGACAACA 
TTGACTGGGC GGACGGTTGC TATTCCTCAG CCTCGCCAGA GGTCCCGGTC TGAGAGTGAT 
GTGAGCAGTG TTGAACAGGA CAGCTTCATC GAGCCCTATG CCACCACCTC ACAGCTGAGG 
CCTCGGCCAA ATTGGCAGAG TGAGATGGGA AGAAGATCTT CTTTGCCATC CTTTGAAACA 
CTGGACTATG GGGACGAAGA GGACATTGAA ACTCAGCTGT CATCCAGCGG CAAGGAATTG 
GGGGATGTCA GTGCCCGGGA GGACAGAGGA GGCCACAGTG ATGACCTGTA CGCTGTGCCA 
CACAGAAATC AGGTGCCATT GTTACATGAG GTGAACAGTG AAGACGATGA AAATATTTCT 
CATCAAGATG GGTTTCCAGG CTCCCCTCCT GCACCACAGC GGACACAACA AAAAGATGGT 
AAACACCCTG TTCTGAATTT AAAGGATGAA AAACCTCCAT TATGTGAGAA ACCTCCACCC 
TCCCCAGATA TAACTGGTAG AGCACGTCAA AGATATACAG AAATAACCAG AGAAAAGTTT 
GAGGCATTAA AAGAAGAAAA TATGGACCTA AACAATATGA ATCAAAGCCT TACCCTTGAA 
CTAAACACAA TGAAACAAGC AATGAAAGAA CTACAGTTAA AACTTAAGGG AATGGAAAAA 
GAGAAGAGAA AGCTCAAAGA GGCTGAGAAG GCGTCGTCAC AGGAAGTTGC TGCACCTGAA 
TTACTTTATC TGCGAAAACA AGCTCAAGAA CTGGTGGATG AAAATGATGG ATTGAAAATG 
ACTGTCCATC GTTTGAATGT AGAACTCAGT CGATATCAGA CAAAATTCAG GCATTTGTCC 
AAGGAAGAGA GCTTAAATAT TGAAGGCCTC CCATCCAAGG GCCCTATACC ACCCTGGTTG 
TTGGATATAA AGTACCTGTC ACCATTGTTG CTGGCTTATG AAGATATGAT GAAAGAGAAG 
GACGAGCTCA ATGCCACCCT CAAGGCGTCA GCTTCAGACT CAAGCAAAAC TGGTTTTAGA 
GGAAAACAAG TTGTTGCTGG AGCAGTTGGA GATTCAGCAA AGGAAAGCCA AGGACAGCCA 
CCAGGAGCGC CTCCAAGAAG AGCTGCGGGC CTGGGAGGCG ACATCACTGC AGTTTCTAAG 
CTGACTAAAC AACTAATGCT CCTGGAGGCA AAAACCCACG GCCAGGAAAA GGAGCTGGCG 
GAGAACAGGG AACAGCTGGA GATTTTACGT GCCAAATGCC AAGAACTCAA AACACACTCG 
GATGGCAAAA TCGCAGTGGA AGTTCATAAA TCAATTGTGA ATGAATTAAA AAGCCAATTA 
CAGAAGGAAG AAGAGAAAGA AAGGGCTGAG ATGGAGGAGT TGATGGAGAA GCTGACAGTC 
CTGCAAGCGC AGAAGAAGAG CCTGCTGTTA GAGAAGAACA GTTTGACAGA GCAAAACAAA 
GCACTGGAAG CCGAACTTGA ACGAGCACAG AAAATCAATA GGAAATCTCA AAAGAAAATT 
GAGGTCCTCA AAAAGCAGGT GGAAAAAGCC ATGGGGAACG AAATGTCTGC TCATCAGTAC 
CTGGCAAACC TTGTTGGCCT GGCAGAAAAC ATAACCCAGG AACGTGACAG TCTTATGTGT 
TTGGCAAAAT GTTTAGAAAG TGAGAAGGAT GGAGTGCTTA ATAAAGTCAT AAAAAGCAAC 
ATTCGCCTGG GAAAGTTAGA GGAAAAAGTC AAGGGCTACA AGAAGCAGGC AGCACTGAAG 
CTGGGGGACA TCAGTCACCG TCTGCTGGAG CAGCAGGAGG ACTTCGCCGG CAAGACAGCC 
CAGTACCGGC AGGAGATGCG GCACCTGCAC CAGGTGCTGA AGGACAAGCA GGAGGTGCTG 
GACCAGGCGC TGCAGCAGAA CAGAGAAATG GAAGGTGAAC TTGAAGTTAT TTGGGAATCT 
ACCTTCAGGG AAAACCGAAG AATCCGAGAA CTTCTCCAGG ACACACTCAC GAGGACAGGC 
GTGCAGGACA ACCCCAGAGC TCTGGTTGCC CCCAGCCTCA ATGGCGTCTC TCAGGCAGAC 
CTGCTGGACG GCTGCGATGT CTGCTCCTAT GACCTGAAGT CTCATGCCCC CACCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005259401.1
CDS	151..2430
Translation	MLLGFRRGRRSHFKHIIHGLLPAASVAPKAAVPRTPPPRSPNPSPERPRSALAAAILATTLTGRTVAIPQPRQRSRSESDVSSVEQDSFIEPYATTSQLRPRPNWQSEMGRRSSLPSFETLDYGDEEDIETQLSSSGKELGDVSAREDRGGHSDDLYAVPHRNQVPLLHEVNSEDDENISHQDGFPGSPPAPQRTQQKDGKHPVLNLKDEKPPLCEKPPPSPDITGRARQRYTEITREKFEALKEENMDLNNMNQSLTLELNTMKQAMKELQLKLKGMEKEKRKLKEAEKASSQEVAAPELLYLRKQAQELVDENDGLKMTVHRLNVELSRYQTKFRHLSKEESLNIEGLPSKGPIPPWLLDIKYLSPLLLAYEDMMKEKDELNATLKASASDSSKTGFRGKQVVAGAVGDSAKESQGQPPGAPPRRAAGLGGDITAVSKLTKQLMLLEAKTHGQEKELAENREQLEILRAKCQELKTHSDGKIAVEVHKSIVNELKSQLQKEEEKERAEMEELMEKLTVLQAQKKSLLLEKNSLTEQNKALEAELERAQKINRKSQKKIEVLKKQVEKAMGNEMSAHQYLANLVGLAENITQERDSLMCLAKCLESEKDGVLNKVIKSNIRLGKLEEKVKGYKKQAALKLGDISHRLLEQQEDFAGKTAQYRQEMRHLHQVLKDKQEVLDQALQQNREMEGELEVIWESTFRENRRIRELLQDTLTRTGVQDNPRALVAPSLNGVSQADLLDGCDVCSYDLKSHAPTC

Target ORF information:

RefSeq Version	XM_005259344.3
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 89 (CEP89), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005259344.3

ORF Insert Sequence:

ATGCTCCTGG GATTTCGGAG AGGCCGCAGG AGTCATTTCA AACACATCAT CCATGGCCTT 
TTACCTGCAG CCAGCGTTGC TCCGAAGGCA GCTGTGCCAC GCACACCTCC TCCCCGCAGC 
CCCAACCCAT CTCCAGAGAG ACCAAGATCT GCTCTGGCAG CAGCCATTCT GGCGACAACA 
TTGACTGGGC GGACGGTTGC TATTCCTCAG CCTCGCCAGA GGTCCCGGTC TGAGAGTGAT 
GTGAGCAGTG TTGAACAGGA CAGCTTCATC GAGCCCTATG CCACCACCTC ACAGCTGAGG 
CCTCGGCCAA ATTGGCAGAG TGAGATGGGA AGAAGATCTT CTTTGCCATC CTTTGAAACA 
CTGGACTATG GGGACGAAGA GGACATTGAA ACTCAGCTGT CATCCAGCGG CAAGGAATTG 
GGGGATGTCA GTGCCCGGGA GGACAGAGGA GGCCACAGTG ATGACCTGTA CGCTGTGCCA 
CACAGAAATC AGGTGCCATT GTTACATGAG GTGAACAGTG AAGACGATGA AAATATTTCT 
CATCAAGATG GGTTTCCAGG CTCCCCTCCT GCACCACAGC GGACACAACA AAAAGATGGT 
AAACACCCTG TTCTGAATTT AAAGGATGAA AAACCTCCAT TATGTGAGAA ACCTCCACCC 
TCCCCAGATA TAACTGGTAG AGCACGTCAA AGATATACAG AAATAACCAG AGAAAAGTTT 
GAGGCATTAA AAGAAGAAAA TATGGACCTA AACAATATGA ATCAAAGCCT TACCCTTGAA 
CTAAACACAA TGAAACAAGC AATGAAAGAA CTACAGTTAA AACTTAAGGG AATGGAAAAA 
GAGAAGAGAA AGCTCAAAGA GGCTGAGAAG GCGTCGTCAC AGGAAGTTGC TGCACCTGAA 
TTACTTTATC TGCGAAAACA AGCTCAAGAA CTGGTGGATG AAAATGATGG ATTGAAAATG 
ACTGTCCATC GTTTGAATGT AGAACTCAGT CGATATCAGA CAAAATTCAG GCATTTGTCC 
AAGGAAGAGA GCTTAAATAT TGAAGGCCTC CCATCCAAGG GCCCTATACC ACCCTGGTTG 
TTGGATATAA AGTACCTGTC ACCATTGTTG CTGGCTTATG AAGATATGAT GAAAGAGAAG 
GACGAGCTCA ATGCCACCCT CAAGGCGTCA GCTTCAGACT CAAGCAAAAC TGGTTTTAGA 
GGAAAACAAG TTGTTGCTGG AGCAGTTGGA GATTCAGCAA AGGAAAGCCA AGGACAGCCA 
CCAGGAGCGC CTCCAAGAAG AGCTGCGGGC CTGGGAGGCG ACATCACTGC AGTTTCTAAG 
CTGACTAAAC AACTAATGCT CCTGGAGGCA AAAACCCACG GCCAGGAAAA GGAGCTGGCG 
GAGAACAGGG AACAGCTGGA GATTTTACGT GCCAAATGCC AAGAACTCAA AACACACTCG 
GATGGCAAAA TCGCAGTGGA AGTTCATAAA TCAATTGTGA ATGAATTAAA AAGCCAATTA 
CAGAAGGAAG AAGAGAAAGA AAGGGCTGAG ATGGAGGAGT TGATGGAGAA GCTGACAGTC 
CTGCAAGCGC AGAAGAAGAG CCTGCTGTTA GAGAAGAACA GTTTGACAGA GCAAAACAAA 
GCACTGGAAG CCGAACTTGA ACGAGCACAG AAAATCAATA GGAAATCTCA AAAGAAAATT 
GAGGTCCTCA AAAAGCAGGT GGAAAAAGCC ATGGGGAACG AAATGTCTGC TCATCAGTAC 
CTGGCAAACC TTGTTGGCCT GGCAGAAAAC ATAACCCAGG AACGTGACAG TCTTATGTGT 
TTGGCAAAAT GTTTAGAAAG TGAGAAGGAT GGAGTGCTTA ATAAAGTCAT AAAAAGCAAC 
ATTCGCCTGG GAAAGTTAGA GGAAAAAGTC AAGGGCTACA AGAAGCAGGC AGCACTGAAG 
CTGGGGGACA TCAGTCACCG TCTGCTGGAG CAGCAGGAGG ACTTCGCCGG CAAGACAGCC 
CAGTACCGGC AGGAGATGCG GCACCTGCAC CAGGTGCTGA AGGACAAGCA GGAGGTGCTG 
GACCAGGCGC TGCAGCAGAA CAGAGAAATG GAAGGTGAAC TTGAAGTTAT TTGGGAATCT 
ACCTTCAGGG AAAACCGAAG AATCCGAGAA CTTCTCCAGG ACACACTCAC GAGGACAGGC 
GTGCAGGACA ACCCCAGAGC TCTGGTTGCC CCCAGCCTCA ATGGCGTCTC TCAGGCAGAC 
CTGCTGGACG GCTGCGATGT CTGCTCCTAT GACCTGAAGT CTCATGCCCC CACCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CEP89 ( XM_017027398.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882887.1 Homo sapiens centrosomal protein 89 (CEP89), transcript variant X2, mRNA.

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CloneID	OHu88775
Clone ID Related Accession (Same CDS sequence)	XM_017027398.1
Accession Version	XM_017027398.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	centrosomal protein of 89 kDa isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCCTGG GATTTCGGAG AGGCCGCAGG AGTCATTTCA AACACATCAT CCATGGCCTT 
TTACCTGCAG CCAGCGTTGC TCCGAAGGCA GCTGTGCCAC GCACACCTCC TCCCCGCAGC 
CCCAACCCAT CTCCAGAGAG ACCAAGATCT GCTCTGGCAG CAGCCATTCT GGCGACAACA 
TTGACTGGGC GGACGGTTGC TATTCCTCAG CCTCGCCAGA GGTCCCGGTC TGAGAGTGAT 
GTGAGCAGTG TTGAACAGGA CAGCTTCATC GAGCCCTATG CCACCACCTC ACAGCTGAGG 
CCTCGGCCAA ATTGGCAGAG TGAGATGGGA AGAAGATCTT CTTTGCCATC CTTTGAAACA 
CTGGACTATG GGGACGAAGA GGACATTGAA ACTCAGCTGT CATCCAGCGG CAAGGAATTG 
GGGGATGTCA GTGCCCGGGA GGACAGAGGA GGCCACAGTG ATGACCTGTA CGCTGTGCCA 
CACAGAAATC AGGTGCCATT GTTACATGAG GTGAACAGTG AAGACGATGA AAATATTTCT 
CATCAAGATG GGTTTCCAGG CTCCCCTCCT GCACCACAGC GGACACAACA AAAAGATGGT 
AAACACCCTG TTCTGAATTT AAAGGATGAA AAACCTCCAT TATGTGAGAA ACCTCCACCC 
TCCCCAGATA TAACTGGTAG AGCACGTCAA AGATATACAG AAATAACCAG AGAAAAGTTT 
GAGGCATTAA AAGAAGAAAA TATGGACCTA AACAATATGA ATCAAAGCCT TACCCTTGAA 
CTAAACACAA TGAAACAAGC AATGAAAGAA CTACAGTTAA AACTTAAGGG AATGGAAAAA 
GAGAAGAGAA AGCTCAAAGA GGCTGAGAAG GCGTCGTCAC AGGAAGTTGC TGCACCTGAA 
TTACTTTATC TGCGAAAACA AGCTCAAGAA CTGGTGGATG AAAATGATGG ATTGAAAATG 
ACTGTCCATC GTTTGAATGT AGAACTCAGT CGATATCAGA CAAAATTCAG GCATTTGTCC 
AAGGAAGAGA GCTTAAATAT TGAAGGCCTC CCATCCAAGG GCCCTATACC ACCCTGGTTG 
TTGGATATAA AGTACCTGTC ACCATTGTTG CTGGCTTATG AAGATATGAT GAAAGAGAAG 
GACGAGCTCA ATGCCACCCT CAAGGAGGAA ATGAGAATGT TTAGGATGCG AGTCCAAGAA 
GTGGTGAAAG AAAATGAAGA ATTGCACCAA GAGTTAAATA AGAGTAGTGC TGTTACCAGT 
GAGGAATGGC GTCAGCTTCA GACTCAAGCA AAACTGGTTT TAGAGGAAAA CAAGTTGTTG 
CTGGAGCAGT TGGAGATTCA GCAAAGGAAA GCCAAGGACA GCCACCAGGA GCGCCTCCAA 
GAAGTTTCTA AGCTGACTAA ACAACTAATG CTCCTGGAGG CAAAAACCCA CGGCCAGGAA 
AAGGAGCTGG CGGAGAACAG GGAACAGCTG GAGATTTTAC GTGCCAAATG CCAAGAACTC 
AAAACACACT CGGATGGCAA AATCGCAGTG GAAGTTCATA AATCAATTGT GAATGAATTA 
AAAAGCCAAT TACAGAAGGA AGAAGAGAAA GAAAGGGCTG AGATGGAGGA GTTGATGGAG 
AAGCTGACAG TCCTGCAAGC GCAGAAGAAG AGCCTGCTGT TAGAGAAGAA CAGTTTGACA 
GAGCAAAACA AAGCACTGGA AGCCGAACTT GAACGAGCAC AGAAAATCAA TAGGAAATCT 
CAAAAGAAAA TTGAGGTCCT CAAAAAGCAG GTGGAAAAAG CCATGGGGAA CGAAATGTCT 
GCTCATCAGT ACCTGGCAAA CCTTGTTGGC CTGGCAGAAA ACATAACCCA GGAACGTGAC 
AGTCTTATGT GTTTGCCCTT TGAATATATC GTCTCATTCT CTCCTGGCCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882887.1
CDS	151..2064
Translation	MLLGFRRGRRSHFKHIIHGLLPAASVAPKAAVPRTPPPRSPNPSPERPRSALAAAILATTLTGRTVAIPQPRQRSRSESDVSSVEQDSFIEPYATTSQLRPRPNWQSEMGRRSSLPSFETLDYGDEEDIETQLSSSGKELGDVSAREDRGGHSDDLYAVPHRNQVPLLHEVNSEDDENISHQDGFPGSPPAPQRTQQKDGKHPVLNLKDEKPPLCEKPPPSPDITGRARQRYTEITREKFEALKEENMDLNNMNQSLTLELNTMKQAMKELQLKLKGMEKEKRKLKEAEKASSQEVAAPELLYLRKQAQELVDENDGLKMTVHRLNVELSRYQTKFRHLSKEESLNIEGLPSKGPIPPWLLDIKYLSPLLLAYEDMMKEKDELNATLKEEMRMFRMRVQEVVKENEELHQELNKSSAVTSEEWRQLQTQAKLVLEENKLLLEQLEIQQRKAKDSHQERLQEVSKLTKQLMLLEAKTHGQEKELAENREQLEILRAKCQELKTHSDGKIAVEVHKSIVNELKSQLQKEEEKERAEMEELMEKLTVLQAQKKSLLLEKNSLTEQNKALEAELERAQKINRKSQKKIEVLKKQVEKAMGNEMSAHQYLANLVGLAENITQERDSLMCLPFEYIVSFSPGL

Target ORF information:

RefSeq Version	XM_017027398.1
Organism	Homo sapiens(human)
Definition	Homo sapiens centrosomal protein 89 (CEP89), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017027398.1

ORF Insert Sequence:

ATGCTCCTGG GATTTCGGAG AGGCCGCAGG AGTCATTTCA AACACATCAT CCATGGCCTT 
TTACCTGCAG CCAGCGTTGC TCCGAAGGCA GCTGTGCCAC GCACACCTCC TCCCCGCAGC 
CCCAACCCAT CTCCAGAGAG ACCAAGATCT GCTCTGGCAG CAGCCATTCT GGCGACAACA 
TTGACTGGGC GGACGGTTGC TATTCCTCAG CCTCGCCAGA GGTCCCGGTC TGAGAGTGAT 
GTGAGCAGTG TTGAACAGGA CAGCTTCATC GAGCCCTATG CCACCACCTC ACAGCTGAGG 
CCTCGGCCAA ATTGGCAGAG TGAGATGGGA AGAAGATCTT CTTTGCCATC CTTTGAAACA 
CTGGACTATG GGGACGAAGA GGACATTGAA ACTCAGCTGT CATCCAGCGG CAAGGAATTG 
GGGGATGTCA GTGCCCGGGA GGACAGAGGA GGCCACAGTG ATGACCTGTA CGCTGTGCCA 
CACAGAAATC AGGTGCCATT GTTACATGAG GTGAACAGTG AAGACGATGA AAATATTTCT 
CATCAAGATG GGTTTCCAGG CTCCCCTCCT GCACCACAGC GGACACAACA AAAAGATGGT 
AAACACCCTG TTCTGAATTT AAAGGATGAA AAACCTCCAT TATGTGAGAA ACCTCCACCC 
TCCCCAGATA TAACTGGTAG AGCACGTCAA AGATATACAG AAATAACCAG AGAAAAGTTT 
GAGGCATTAA AAGAAGAAAA TATGGACCTA AACAATATGA ATCAAAGCCT TACCCTTGAA 
CTAAACACAA TGAAACAAGC AATGAAAGAA CTACAGTTAA AACTTAAGGG AATGGAAAAA 
GAGAAGAGAA AGCTCAAAGA GGCTGAGAAG GCGTCGTCAC AGGAAGTTGC TGCACCTGAA 
TTACTTTATC TGCGAAAACA AGCTCAAGAA CTGGTGGATG AAAATGATGG ATTGAAAATG 
ACTGTCCATC GTTTGAATGT AGAACTCAGT CGATATCAGA CAAAATTCAG GCATTTGTCC 
AAGGAAGAGA GCTTAAATAT TGAAGGCCTC CCATCCAAGG GCCCTATACC ACCCTGGTTG 
TTGGATATAA AGTACCTGTC ACCATTGTTG CTGGCTTATG AAGATATGAT GAAAGAGAAG 
GACGAGCTCA ATGCCACCCT CAAGGAGGAA ATGAGAATGT TTAGGATGCG AGTCCAAGAA 
GTGGTGAAAG AAAATGAAGA ATTGCACCAA GAGTTAAATA AGAGTAGTGC TGTTACCAGT 
GAGGAATGGC GTCAGCTTCA GACTCAAGCA AAACTGGTTT TAGAGGAAAA CAAGTTGTTG 
CTGGAGCAGT TGGAGATTCA GCAAAGGAAA GCCAAGGACA GCCACCAGGA GCGCCTCCAA 
GAAGTTTCTA AGCTGACTAA ACAACTAATG CTCCTGGAGG CAAAAACCCA CGGCCAGGAA 
AAGGAGCTGG CGGAGAACAG GGAACAGCTG GAGATTTTAC GTGCCAAATG CCAAGAACTC 
AAAACACACT CGGATGGCAA AATCGCAGTG GAAGTTCATA AATCAATTGT GAATGAATTA 
AAAAGCCAAT TACAGAAGGA AGAAGAGAAA GAAAGGGCTG AGATGGAGGA GTTGATGGAG 
AAGCTGACAG TCCTGCAAGC GCAGAAGAAG AGCCTGCTGT TAGAGAAGAA CAGTTTGACA 
GAGCAAAACA AAGCACTGGA AGCCGAACTT GAACGAGCAC AGAAAATCAA TAGGAAATCT 
CAAAAGAAAA TTGAGGTCCT CAAAAAGCAG GTGGAAAAAG CCATGGGGAA CGAAATGTCT 
GCTCATCAGT ACCTGGCAAA CCTTGTTGGC CTGGCAGAAA ACATAACCCA GGAACGTGAC 
AGTCTTATGT GTTTGCCCTT TGAATATATC GTCTCATTCT CTCCTGGCCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CEP89 is required for mitochondrial metabolism and neuronal function in man and fly.
Human molecular genetics22(15)3138-51(2013 Aug)
van Bon BW,Oortveld MA,Nijtmans LG,Fenckova M,Nijhof B,Besseling J,Vos M,Kramer JM,de Leeuw N,Castells-Nobau A,Asztalos L,Viragh E,Ruiter M,Hofmann F,Eshuis L,Collavin L,Huynen MA,Asztalos Z,Verstreken P,Rodenburg RJ,Smeitink JA,de Vries BB,Schenck A

Primary ciliogenesis requires the distal appendage component Cep123.
Biology open2(6)535-45(2013 Jun)
Sillibourne JE,Hurbain I,Grand-Perret T,Goud B,Tran P,Bornens M

Centriole distal appendages promote membrane docking, leading to cilia initiation.
Genes & development27(2)163-8(2013 Jan)
Tanos BE,Yang HJ,Soni R,Wang WJ,Macaluso FP,Asara JM,Tsou MF

Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods.
The EMBO journal30(8)1520-35(2011 Apr)
Jakobsen L,Vanselow K,Skogs M,Toyoda Y,Lundberg E,Poser I,Falkenby LG,Bennetzen M,Westendorf J,Nigg EA,Uhlen M,Hyman AA,Andersen JS

Genetic loci influencing kidney function and chronic kidney disease.
Nature genetics42(5)373-5(2010 May)
Chambers JC,Zhang W,Lord GM,van der Harst P,Lawlor DA,Sehmi JS,Gale DP,Wass MN,Ahmadi KR,Bakker SJ,Beckmann J,Bilo HJ,Bochud M,Brown MJ,Caulfield MJ,Connell JM,Cook HT,Cotlarciuc I,Davey Smith G,de Silva R,Deng G,Devuyst O,Dikkeschei LD,Dimkovic N,Dockrell M,Dominiczak A,Ebrahim S,Eggermann T,Farrall M,Ferrucci L,Floege J,Forouhi NG,Gansevoort RT,Han X,Hedblad B,Homan van der Heide JJ,Hepkema BG,Hernandez-Fuentes M,Hypponen E,Johnson T,de Jong PE,Kleefstra N,Lagou V,Lapsley M,Li Y,Loos RJ,Luan J,Luttropp K,Mar?chal C,Melander O,Munroe PB,Nordfors L,Parsa A,Peltonen L,Penninx BW,Perucha E,Pouta A,Prokopenko I,Roderick PJ,Ruokonen A,Samani NJ,Sanna S,Schalling M,Schlessinger D,Schlieper G,Seelen MA,Shuldiner AR,Sj?gren M,Smit JH,Snieder H,Soranzo N,Spector TD,Stenvinkel P,Sternberg MJ,Swaminathan R,Tanaka T,Ubink-Veltmaat LJ,Uda M,Vollenweider P,Wallace C,Waterworth D,Zerres K,Waeber G,Wareham NJ,Maxwell PH,McCarthy MI,Jarvelin MR,Mooser V,Abecasis GR,Lightstone L,Scott J,Navis G,Elliott P,Kooner JS

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CEP89 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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