TRIM63 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRIM63
Entrez Gene ID	84676
Full Name	tripartite motif containing 63
Synonyms	IRF,MURF1,MURF2,RNF28,SMRZ
General protein information	Preferred Names tripartite motif containing 63 Names E3 ubiquitin-protein ligase TRIM63 RING-type E3 ubiquitin transferase TRIM63 iris ring finger protein muscle specific ring finger protein 2 muscle-specific RING finger protein 1 ring finger protein 28 striated muscle RING zinc finger protein tripartite motif containing 63, E3 ubiquitin protein ligase tripartite motif-containing protein 63
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.11
Summary	This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is an E3 ubiquitin ligase that localizes to the Z-line and M-line lattices of myofibrils. This protein plays an important role in the atrophy of skeletal and cardiac muscle and is required for the degradation of myosin heavy chain proteins, myosin light chain, myosin binding protein, and for muscle-type creatine kinase. [provided by RefSeq, Feb 2012].
Disorder MIM:	606131

mRNA and Protein(s)

mRNA	Protein	Name
NM_032588.3	NP_115977.2	E3 ubiquitin-protein ligase TRIM63
XM_017002559.2	XP_016858048.1	E3 ubiquitin-protein ligase TRIM63 isoform X1
XM_017002559.1	XP_016858048.1	E3 ubiquitin-protein ligase TRIM63 isoform X1

Pathways from BioSystems

Homologies

	TRIM63	XP_001108306.1
Bos taurus (cattle)	TRIM63	NP_001039760.1
Mus musculus (house mouse)	Trim63	NP_001034137.2
Rattus norvegicus (Norway rat)	Trim63	NP_543179.1
Canis lupus familiaris (dog)	TRIM63	XP_005617821.1
Xenopus tropicalis (tropical clawed frog)	trim63	NP_001072557.1
Homo sapiens (human)	TRIM63	NP_115977.2
Pan troglodytes (chimpanzee)	TRIM63	XP_524625.1
Gallus gallus (chicken)	TRIM63	XP_424369.4

Gene Ontology
Bibliography

Related articles in PubMed

TRIM63 (MuRF-1) Gene Polymorphism is Associated with Biomarkers of Exercise-Induced Muscle Damage.
Baumert P, Lake MJ, Drust B, Stewart C, Erskine RM
Physiological genomics(2017 Dec)

STING-IRF3 Triggers Endothelial Inflammation in Response to Free Fatty Acid-Induced Mitochondrial Damage in Diet-Induced Obesity.
Mao Y, Luo W, Zhang L, Wu W, Yuan L, Xu H, Song J, Fujiwara K, Abe JI, LeMaire SA, Wang XL, Shen YH
Arteriosclerosis, thrombosis, and vascular biology37(5)920-929(2017 May)

The role of E3 ubiquitin-ligases MuRF-1 and MAFbx in loss of skeletal muscle mass.
Rom O, Reznick AZ
Free radical biology & medicine98218-230(2016 09)

Transcriptional activator TAp63 is upregulated in muscular atrophy during ALS and induces the pro-atrophic ubiquitin ligase Trim63.
von Grabowiecki Y, Abreu P, Blanchard O, Palamiuc L, Benosman S, M?riaux S, Devignot V, Gross I, Mellitzer G, Gonzalez de Aguilar JL, Gaiddon C
eLife5(2016 Feb)

Involvement of the FoxO1/MuRF1/Atrogin-1 Signaling Pathway in the Oxidative Stress-Induced Atrophy of Cultured Chronic Obstructive Pulmonary Disease Myotubes.
Pomi?s P, Blaqui?re M, Maury J, Mercier J, Gouzi F, Hayot M
PloS one11(8)e0160092(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The mitochondrial damage-cGAS-STING-IRF3 pathway is critically involved in metabolic stress-induced endothelial inflammation.
Title: STING-IRF3 Triggers Endothelial Inflammation in Response to Free Fatty Acid-Induced Mitochondrial Damage in Diet-Induced Obesity.

role of the muscle specific E3s MuRF-1 and MAFbx in skeletal muscle wasting during various pathologies, as well as their regulation by modifiable lifestyle factors, were explored (review)
Title: The role of E3 ubiquitin-ligases MuRF-1 and MAFbx in loss of skeletal muscle mass.

Altogether, these results suggest a novel function for p63 as a contributor to muscular atrophic processes via the regulation of multiple genes, including the muscle atrophy gene Trim63.
Title: Transcriptional activator TAp63 is upregulated in muscular atrophy during ALS and induces the pro-atrophic ubiquitin ligase Trim63.

the involvement of oxidative stress in the atrophy of COPD peripheral muscle cells in vitro, via the FoxO1/MuRF1/atrogin-1 signaling pathway of the ubiquitin/proteasome system
Title: Involvement of the FoxO1/MuRF1/Atrogin-1 Signaling Pathway in the Oxidative Stress-Induced Atrophy of Cultured Chronic Obstructive Pulmonary Disease Myotubes.

Skeletal muscle atrophy induced by Angiotensin II involves activation of MuRF1 expression.
Title: Angiotensin II Induces Skeletal Muscle Atrophy by Activating TFEB-Mediated MuRF1 Expression.

The following TRIM63 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRIM63 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26876	NM_032588.3 Latest version!	Homo sapiens tripartite motif containing 63 (TRIM63), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu90666	XM_017002559.2 Latest version!	Homo sapiens tripartite motif containing 63 (TRIM63), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu90666	XM_017002559.1	Homo sapiens tripartite motif containing 63 (TRIM63), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRIM63 ( NM_032588.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_115977.2 Homo sapiens tripartite motif containing 63 (TRIM63), mRNA.

Price & Availability↑

CloneID	OHu26876
Clone ID Related Accession (Same CDS sequence)	NM_032588.3
Accession Version	NM_032588.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIM63
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA900348.1, BC080529.1 and AI351421.1. This sequence is a reference standard in the RefSeqGene project. On Feb 28, 2012 this sequence version replaced NM_032588.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF353673.1, AK056942.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267764 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGATTATA AGTCGAGCCT GATCCAGGAT GGGAATCCCA TGGAGAACTT GGAGAAGCAG 
CTGATCTGCC CTATCTGCCT GGAGATGTTT ACCAAGCCAG TGGTCATCTT GCCGTGCCAG 
CACAACCTGT GCCGGAAGTG TGCCAATGAC ATCTTCCAGG CTGCAAATCC CTACTGGACC 
AGCCGGGGCA GCTCAGTGTC CATGTCTGGA GGCCGTTTCC GCTGCCCCAC CTGCCGCCAC 
GAGGTGATCA TGGATCGTCA CGGAGTGTAC GGCCTGCAGA GGAACCTGCT GGTGGAGAAC 
ATCATCGACA TCTACAAACA GGAGTGCTCC AGTCGGCCGC TGCAGAAGGG CAGTCACCCC 
ATGTGCAAGG AGCACGAAGA TGAGAAAATC AACATCTACT GTCTCACGTG TGAGGTGCCC 
ACCTGCTCCA TGTGCAAGGT GTTTGGGATC CACAAGGCCT GCGAGGTGGC CCCATTGCAG 
AGTGTCTTCC AGGGACAAAA GACTGAACTG AATAACTGTA TCTCCATGCT GGTGGCGGGG 
AATGACCGTG TGCAGACCAT CATCACTCAG CTGGAGGATT CCCGTCGAGT GACCAAGGAG 
AACAGTCACC AGGTAAAGGA AGAGCTGAGC CAGAAGTTTG ACACGTTGTA TGCCATCCTG 
GATGAGAAGA AAAGTGAGTT GCTGCAGCGG ATCACGCAGG AGCAGGAGAA AAAGCTTAGC 
TTCATCGAGG CCCTCATCCA GCAGTACCAG GAGCAGCTGG ACAAGTCCAC AAAGCTGGTG 
GAAACTGCCA TCCAGTCCCT GGACGAGCCT GGGGGAGCCA CCTTCCTCTT GACTGCCAAG 
CAACTCATCA AAAGCATTGT GGAAGCTTCC AAGGGCTGCC AGCTGGGGAA GACAGAGCAG 
GGCTTTGAGA ACATGGACTT CTTTACTTTG GATTTAGAGC ACATAGCAGA CGCCCTGAGA 
GCCATTGACT TTGGGACAGA TGAGGAAGAG GAAGAATTCA TTGAAGAAGA AGATCAGGAA 
GAGGAAGAGT CCACAGAAGG GAAGGAAGAA GGACACCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115977.2
CDS	141..1202
Translation	MDYKSSLIQDGNPMENLEKQLICPICLEMFTKPVVILPCQHNLCRKCANDIFQAANPYWTSRGSSVSMSGGRFRCPTCRHEVIMDRHGVYGLQRNLLVENIIDIYKQECSSRPLQKGSHPMCKEHEDEKINIYCLTCEVPTCSMCKVFGIHKACEVAPLQSVFQGQKTELNNCISMLVAGNDRVQTIITQLEDSRRVTKENSHQVKEELSQKFDTLYAILDEKKSELLQRITQEQEKKLSFIEALIQQYQEQLDKSTKLVETAIQSLDEPGGATFLLTAKQLIKSIVEASKGCQLGKTEQGFENMDFFTLDLEHIADALRAIDFGTDEEEEEFIEEEDQEEEESTEGKEEGHQ

Target ORF information:

RefSeq Version	NM_032588.3
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 63 (TRIM63), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032588.3

ORF Insert Sequence:

ATGGATTATA AGTCGAGCCT GATCCAGGAT GGGAATCCCA TGGAGAACTT GGAGAAGCAG 
CTGATCTGCC CTATCTGCCT GGAGATGTTT ACCAAGCCAG TGGTCATCTT GCCGTGCCAG 
CACAACCTGT GCCGGAAGTG TGCCAATGAC ATCTTCCAGG CTGCAAATCC CTACTGGACC 
AGCCGGGGCA GCTCAGTGTC CATGTCTGGA GGCCGTTTCC GCTGCCCCAC CTGCCGCCAC 
GAGGTGATCA TGGATCGTCA CGGAGTGTAC GGCCTGCAGA GGAACCTGCT GGTGGAGAAC 
ATCATCGACA TCTACAAACA GGAGTGCTCC AGTCGGCCGC TGCAGAAGGG CAGTCACCCC 
ATGTGCAAGG AGCACGAAGA TGAGAAAATC AACATCTACT GTCTCACGTG TGAGGTGCCC 
ACCTGCTCCA TGTGCAAGGT GTTTGGGATC CACAAGGCCT GCGAGGTGGC CCCATTGCAG 
AGTGTCTTCC AGGGACAAAA GACTGAACTG AATAACTGTA TCTCCATGCT GGTGGCGGGG 
AATGACCGTG TGCAGACCAT CATCACTCAG CTGGAGGATT CCCGTCGAGT GACCAAGGAG 
AACAGTCACC AGGTAAAGGA AGAGCTGAGC CAGAAGTTTG ACACGTTGTA TGCCATCCTG 
GATGAGAAGA AAAGTGAGTT GCTGCAGCGG ATCACGCAGG AGCAGGAGAA AAAGCTTAGC 
TTCATCGAGG CCCTCATCCA GCAGTACCAG GAGCAGCTGG ACAAGTCCAC AAAGCTGGTG 
GAAACTGCCA TCCAGTCCCT GGACGAGCCT GGGGGAGCCA CCTTCCTCTT GACTGCCAAG 
CAACTCATCA AAAGCATTGT GGAAGCTTCC AAGGGCTGCC AGCTGGGGAA GACAGAGCAG 
GGCTTTGAGA ACATGGACTT CTTTACTTTG GATTTAGAGC ACATAGCAGA CGCCCTGAGA 
GCCATTGACT TTGGGACAGA TGAGGAAGAG GAAGAATTCA TTGAAGAAGA AGATCAGGAA 
GAGGAAGAGT CCACAGAAGG GAAGGAAGAA GGACACCAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM63 ( XM_017002559.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858048.1 Homo sapiens tripartite motif containing 63 (TRIM63), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu90666
Clone ID Related Accession (Same CDS sequence)	XM_017002559.2 , XM_017002559.1
Accession Version	XM_017002559.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIM63 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017002559.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTATA AGTCGAGCCT GATCCAGGAT GGGAATCCCA TGGAGAACTT GGAGAAGCAG 
CTGATCTGCC CTATCTGCCT GGAGATGTTT ACCAAGCCAG TGGTCATCTT GCCGTGCCAG 
CACAACCTGT GCCGGAAGTG TGCCAATGAC ATCTTCCAGG CTGCAAATCC CTACTGGACC 
AGCCGGGGCA GCTCAGTGTC CATGTCTGGA GGCCGTTTCC GCTGCCCCAC CTGCCGCCAC 
GAGGTGATCA TGGATCGTCA CGGAGTGTAC GGCCTGCAGA GGAACCTGCT GGTGGAGAAC 
ATCATCGACA TCTACAAACA GGAGTGCTCC AGTCGGCCGC TGCAGAAGGG CAGTCACCCC 
ATGTGCAAGG AGCACGAAGA TGAGAAAATC AACATCTACT GTCTCACGTG TGAGGTGCCC 
ACCTGCTCCA TGTGCAAGGT GTTTGGGATC CACAAGGCCT GCGAGGTGGC CCCATTGCAG 
AGTGTCTTCC AGGGACAAAA GACTGAACTG AATAACTGTA TCTCCATGCT GGTGGCGGGG 
AATGACCGTG TGCAGACCAT CATCACTCAG CTGGAGGATT CCCGTCGAGT GACCAAGGAG 
AACAGTCACC AGGTAAAGGA AGAGCTGAGC CAGAAGTTTG ACACGTTGTA TGCCATCCTG 
GATGAGAAGA AAAGTGAGTT GCTGCAGCGG ATCACGCAGG AGCAGGAGAA AAAGCTTAGC 
TTCATCGAGG CCCTCATCCA GCAGTACCAG GAGCAGCTGG ACAAGTCCAC AAAGCTGGTG 
GAAACTGCCA TCCAGTCCCT GGACGAGCCT GGGGGAGCCA CCTTCCTCTT GACTGCCAAG 
CAACTCATCA AAAGCATTGT GGAAGCTTCC AAGGGCTGCC AGCTGGGGAA GACAGAGCAG 
GGCTTTGAGA ACATGGACTT CTTTACTTTG GATTTAGAGC ACATAGCAGA CGCCCTGAGA 
GCCATTGACT TTGGGACAGA TGAGGAAGAG GAAGAATTCA TTGAAGAAGA AGATCAGGAA 
GAGGAAGAGT CCACAGAAGG GAAGGAAGAA GGTAAGAAAT TCATCTACCT CCATTCCTTC 
GTTCCTTCAT TCATTCCACA ACAATCTGAG GTGCCTACTG TATGTGAAGC ACTGTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858048.1
CDS	140..1279
Translation	MDYKSSLIQDGNPMENLEKQLICPICLEMFTKPVVILPCQHNLCRKCANDIFQAANPYWTSRGSSVSMSGGRFRCPTCRHEVIMDRHGVYGLQRNLLVENIIDIYKQECSSRPLQKGSHPMCKEHEDEKINIYCLTCEVPTCSMCKVFGIHKACEVAPLQSVFQGQKTELNNCISMLVAGNDRVQTIITQLEDSRRVTKENSHQVKEELSQKFDTLYAILDEKKSELLQRITQEQEKKLSFIEALIQQYQEQLDKSTKLVETAIQSLDEPGGATFLLTAKQLIKSIVEASKGCQLGKTEQGFENMDFFTLDLEHIADALRAIDFGTDEEEEEFIEEEDQEEEESTEGKEEGKKFIYLHSFVPSFIPQQSEVPTVCEALC

Target ORF information:

RefSeq Version	XM_017002559.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 63 (TRIM63), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002559.2

ORF Insert Sequence:

ATGGATTATA AGTCGAGCCT GATCCAGGAT GGGAATCCCA TGGAGAACTT GGAGAAGCAG 
CTGATCTGCC CTATCTGCCT GGAGATGTTT ACCAAGCCAG TGGTCATCTT GCCGTGCCAG 
CACAACCTGT GCCGGAAGTG TGCCAATGAC ATCTTCCAGG CTGCAAATCC CTACTGGACC 
AGCCGGGGCA GCTCAGTGTC CATGTCTGGA GGCCGTTTCC GCTGCCCCAC CTGCCGCCAC 
GAGGTGATCA TGGATCGTCA CGGAGTGTAC GGCCTGCAGA GGAACCTGCT GGTGGAGAAC 
ATCATCGACA TCTACAAACA GGAGTGCTCC AGTCGGCCGC TGCAGAAGGG CAGTCACCCC 
ATGTGCAAGG AGCACGAAGA TGAGAAAATC AACATCTACT GTCTCACGTG TGAGGTGCCC 
ACCTGCTCCA TGTGCAAGGT GTTTGGGATC CACAAGGCCT GCGAGGTGGC CCCATTGCAG 
AGTGTCTTCC AGGGACAAAA GACTGAACTG AATAACTGTA TCTCCATGCT GGTGGCGGGG 
AATGACCGTG TGCAGACCAT CATCACTCAG CTGGAGGATT CCCGTCGAGT GACCAAGGAG 
AACAGTCACC AGGTAAAGGA AGAGCTGAGC CAGAAGTTTG ACACGTTGTA TGCCATCCTG 
GATGAGAAGA AAAGTGAGTT GCTGCAGCGG ATCACGCAGG AGCAGGAGAA AAAGCTTAGC 
TTCATCGAGG CCCTCATCCA GCAGTACCAG GAGCAGCTGG ACAAGTCCAC AAAGCTGGTG 
GAAACTGCCA TCCAGTCCCT GGACGAGCCT GGGGGAGCCA CCTTCCTCTT GACTGCCAAG 
CAACTCATCA AAAGCATTGT GGAAGCTTCC AAGGGCTGCC AGCTGGGGAA GACAGAGCAG 
GGCTTTGAGA ACATGGACTT CTTTACTTTG GATTTAGAGC ACATAGCAGA CGCCCTGAGA 
GCCATTGACT TTGGGACAGA TGAGGAAGAG GAAGAATTCA TTGAAGAAGA AGATCAGGAA 
GAGGAAGAGT CCACAGAAGG GAAGGAAGAA GGTAAGAAAT TCATCTACCT CCATTCCTTC 
GTTCCTTCAT TCATTCCACA ACAATCTGAG GTGCCTACTG TATGTGAAGC ACTGTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRIM63 ( XM_017002559.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016858048.1 Homo sapiens tripartite motif containing 63 (TRIM63), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu90666
Clone ID Related Accession (Same CDS sequence)	XM_017002559.2 , XM_017002559.1
Accession Version	XM_017002559.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase TRIM63 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTATA AGTCGAGCCT GATCCAGGAT GGGAATCCCA TGGAGAACTT GGAGAAGCAG 
CTGATCTGCC CTATCTGCCT GGAGATGTTT ACCAAGCCAG TGGTCATCTT GCCGTGCCAG 
CACAACCTGT GCCGGAAGTG TGCCAATGAC ATCTTCCAGG CTGCAAATCC CTACTGGACC 
AGCCGGGGCA GCTCAGTGTC CATGTCTGGA GGCCGTTTCC GCTGCCCCAC CTGCCGCCAC 
GAGGTGATCA TGGATCGTCA CGGAGTGTAC GGCCTGCAGA GGAACCTGCT GGTGGAGAAC 
ATCATCGACA TCTACAAACA GGAGTGCTCC AGTCGGCCGC TGCAGAAGGG CAGTCACCCC 
ATGTGCAAGG AGCACGAAGA TGAGAAAATC AACATCTACT GTCTCACGTG TGAGGTGCCC 
ACCTGCTCCA TGTGCAAGGT GTTTGGGATC CACAAGGCCT GCGAGGTGGC CCCATTGCAG 
AGTGTCTTCC AGGGACAAAA GACTGAACTG AATAACTGTA TCTCCATGCT GGTGGCGGGG 
AATGACCGTG TGCAGACCAT CATCACTCAG CTGGAGGATT CCCGTCGAGT GACCAAGGAG 
AACAGTCACC AGGTAAAGGA AGAGCTGAGC CAGAAGTTTG ACACGTTGTA TGCCATCCTG 
GATGAGAAGA AAAGTGAGTT GCTGCAGCGG ATCACGCAGG AGCAGGAGAA AAAGCTTAGC 
TTCATCGAGG CCCTCATCCA GCAGTACCAG GAGCAGCTGG ACAAGTCCAC AAAGCTGGTG 
GAAACTGCCA TCCAGTCCCT GGACGAGCCT GGGGGAGCCA CCTTCCTCTT GACTGCCAAG 
CAACTCATCA AAAGCATTGT GGAAGCTTCC AAGGGCTGCC AGCTGGGGAA GACAGAGCAG 
GGCTTTGAGA ACATGGACTT CTTTACTTTG GATTTAGAGC ACATAGCAGA CGCCCTGAGA 
GCCATTGACT TTGGGACAGA TGAGGAAGAG GAAGAATTCA TTGAAGAAGA AGATCAGGAA 
GAGGAAGAGT CCACAGAAGG GAAGGAAGAA GGTAAGAAAT TCATCTACCT CCATTCCTTC 
GTTCCTTCAT TCATTCCACA ACAATCTGAG GTGCCTACTG TATGTGAAGC ACTGTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016858048.1
CDS	141..1280
Translation	MDYKSSLIQDGNPMENLEKQLICPICLEMFTKPVVILPCQHNLCRKCANDIFQAANPYWTSRGSSVSMSGGRFRCPTCRHEVIMDRHGVYGLQRNLLVENIIDIYKQECSSRPLQKGSHPMCKEHEDEKINIYCLTCEVPTCSMCKVFGIHKACEVAPLQSVFQGQKTELNNCISMLVAGNDRVQTIITQLEDSRRVTKENSHQVKEELSQKFDTLYAILDEKKSELLQRITQEQEKKLSFIEALIQQYQEQLDKSTKLVETAIQSLDEPGGATFLLTAKQLIKSIVEASKGCQLGKTEQGFENMDFFTLDLEHIADALRAIDFGTDEEEEEFIEEEDQEEEESTEGKEEGKKFIYLHSFVPSFIPQQSEVPTVCEALC

Target ORF information:

RefSeq Version	XM_017002559.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tripartite motif containing 63 (TRIM63), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017002559.1

ORF Insert Sequence:

ATGGATTATA AGTCGAGCCT GATCCAGGAT GGGAATCCCA TGGAGAACTT GGAGAAGCAG 
CTGATCTGCC CTATCTGCCT GGAGATGTTT ACCAAGCCAG TGGTCATCTT GCCGTGCCAG 
CACAACCTGT GCCGGAAGTG TGCCAATGAC ATCTTCCAGG CTGCAAATCC CTACTGGACC 
AGCCGGGGCA GCTCAGTGTC CATGTCTGGA GGCCGTTTCC GCTGCCCCAC CTGCCGCCAC 
GAGGTGATCA TGGATCGTCA CGGAGTGTAC GGCCTGCAGA GGAACCTGCT GGTGGAGAAC 
ATCATCGACA TCTACAAACA GGAGTGCTCC AGTCGGCCGC TGCAGAAGGG CAGTCACCCC 
ATGTGCAAGG AGCACGAAGA TGAGAAAATC AACATCTACT GTCTCACGTG TGAGGTGCCC 
ACCTGCTCCA TGTGCAAGGT GTTTGGGATC CACAAGGCCT GCGAGGTGGC CCCATTGCAG 
AGTGTCTTCC AGGGACAAAA GACTGAACTG AATAACTGTA TCTCCATGCT GGTGGCGGGG 
AATGACCGTG TGCAGACCAT CATCACTCAG CTGGAGGATT CCCGTCGAGT GACCAAGGAG 
AACAGTCACC AGGTAAAGGA AGAGCTGAGC CAGAAGTTTG ACACGTTGTA TGCCATCCTG 
GATGAGAAGA AAAGTGAGTT GCTGCAGCGG ATCACGCAGG AGCAGGAGAA AAAGCTTAGC 
TTCATCGAGG CCCTCATCCA GCAGTACCAG GAGCAGCTGG ACAAGTCCAC AAAGCTGGTG 
GAAACTGCCA TCCAGTCCCT GGACGAGCCT GGGGGAGCCA CCTTCCTCTT GACTGCCAAG 
CAACTCATCA AAAGCATTGT GGAAGCTTCC AAGGGCTGCC AGCTGGGGAA GACAGAGCAG 
GGCTTTGAGA ACATGGACTT CTTTACTTTG GATTTAGAGC ACATAGCAGA CGCCCTGAGA 
GCCATTGACT TTGGGACAGA TGAGGAAGAG GAAGAATTCA TTGAAGAAGA AGATCAGGAA 
GAGGAAGAGT CCACAGAAGG GAAGGAAGAA GGTAAGAAAT TCATCTACCT CCATTCCTTC 
GTTCCTTCAT TCATTCCACA ACAATCTGAG GTGCCTACTG TATGTGAAGC ACTGTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TRIM63 (MuRF-1) Gene Polymorphism is Associated with Biomarkers of Exercise-Induced Muscle Damage.
Physiological genomics(2017 Dec)
Baumert P,Lake MJ,Drust B,Stewart C,Erskine RM

STING-IRF3 Triggers Endothelial Inflammation in Response to Free Fatty Acid-Induced Mitochondrial Damage in Diet-Induced Obesity.
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TRIM63 cDNA ORF clone, Homo sapiens(human)

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