MEGF10 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017009988.1	XP_016865477.1	multiple epidermal growth factor-like domains protein 10 isoform X3
NM_001308119.1	NP_001295048.1	multiple epidermal growth factor-like domains protein 10 isoform b precursor
NM_001308121.1	NP_001295050.1	multiple epidermal growth factor-like domains protein 10 isoform b precursor
NM_001256545.1	NP_001243474.1	multiple epidermal growth factor-like domains protein 10 isoform a precursor
NM_001256545.2	NP_001243474.1	multiple epidermal growth factor-like domains protein 10 isoform a precursor
NM_032446.3	NP_115822.1	multiple epidermal growth factor-like domains protein 10 isoform a precursor
XM_017009987.1	XP_016865476.1	multiple epidermal growth factor-like domains protein 10 isoform X1
XM_011543694.1	XP_011541996.1	multiple epidermal growth factor-like domains protein 10 isoform X2
NM_032446.2	NP_115822.1	multiple epidermal growth factor-like domains protein 10 isoform a precursor

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu12082	NM_032446.3 Latest version!	Homo sapiens multiple EGF like domains 10 (MEGF10), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu12082	NM_001256545.2 Latest version!	Homo sapiens multiple EGF like domains 10 (MEGF10), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu12082	XM_011543694.1 Latest version!	Homo sapiens multiple EGF like domains 10 (MEGF10), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu73554	NM_001308121.1 Latest version!	Homo sapiens multiple EGF like domains 10 (MEGF10), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu73554	NM_001308119.1 Latest version!	Homo sapiens multiple EGF like domains 10 (MEGF10), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu96164	XM_017009988.1 Latest version!	Homo sapiens multiple EGF like domains 10 (MEGF10), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu96163	XM_017009987.1 Latest version!	Homo sapiens multiple EGF like domains 10 (MEGF10), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OHu12082	NM_001256545.1	Homo sapiens multiple EGF like domains 10 (MEGF10), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu12082	NM_032446.2	Homo sapiens multiple EGF like domains 10 (MEGF10), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
Hide Old Accession Versions >>

MEGF10 ( NM_001256545.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243474.1 Homo sapiens multiple EGF like domains 10 (MEGF10), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu12082
Clone ID Related Accession (Same CDS sequence)	NM_001256545.1 , NM_001256545.2 , NM_032446.3 , XM_011543694.1 , NM_032446.2
Accession Version	NM_001256545.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-17
Organism	Homo sapiens(human)
Product	multiple epidermal growth factor-like domains protein 10 isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA240513.1, AC008402.6, AC008682.6, DA738723.1 and AC010424.9. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR749437.1, SRR1660805.16218.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTTATTT CTTTGAACTC ATGCCTGAGC TTTATTTGTT TATTGTTATG CCACTGGATT 
GGGACAGCAT CACCTCTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC 
TCAGTGACTG TGCAAGAGTC ATACCCACAT CCCTTTGATC AAATTTACTA CACGAGCTGC 
ACTGACATTC TAAACTGGTT TAAATGCACG CGGCACAGAG TCAGCTATCG GACAGCCTAT 
CGACATGGGG AGAAGACTAT GTATAGGCGC AAGTCTCAGT GTTGTCCTGG ATTTTATGAA 
AGCGGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGTATTGCT 
CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGAGGGACCA ACTGCTCCAG TGCCTGCGAT 
GGTGATCACT GGGGTCCCCA CTGCACCAGC CGGTGCCAGT GCAAAAATGG GGCTCTGTGC 
AACCCCATCA CCGGGGCTTG CCACTGTGCT GCGGGCTTCC GGGGCTGGCG CTGCGAGGAC 
CGCTGTGAGC AGGGCACCTA TGGTAACGAC TGTCATCAGA GATGCCAGTG CCAGAATGGA 
GCCACCTGCG ACCACGTCAC GGGGGAATGC CGCTGCCCAC CAGGATACAC CGGAGCCTTC 
TGTGAGGATC TTTGTCCTCC TGGTAAACAT GGTCCACAGT GTGAGCAGAG ATGCCCTTGT 
CAAAATGGAG GAGTGTGTCA TCACGTCACT GGAGAATGCT CTTGCCCTTC TGGCTGGATG 
GGCACAGTGT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCCCAAGAA 
TGCCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA 
GGATACACAG GGGAACGGTG CCAGGATGAG TGTCCTGTTG GGACCTATGG CGTTCTCTGT 
GCTGAGACCT GCCAGTGTGT CAACGGAGGG AAGTGTTACC ACGTGAGCGG CGCATGCCTC 
TGTGAAGCAG GCTTTGCTGG CGAGCGCTGC GAAGCACGCC TGTGTCCTGA GGGGCTCTAC 
GGCATCAAAT GTGACAAACG GTGTCCCTGC CACCTGGAAA ACACTCATAG CTGTCACCCC 
ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA TGAGACATGT 
TCTCCTGGAT TCTACGGGGA AGCTTGCCAG CAGATCTGCA GCTGCCAAAA TGGGGCAGAC 
TGTGACAGTG TGACTGGAAA GTGCACCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT 
ACCCCATGCC CTCTGGGAAC CTATGGGATA AACTGTTCCT CTCGCTGTGG CTGTAAAAAT 
GATGCAGTCT GCTCTCCTGT GGACGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG 
GACTGCTCCA TCAGATGTCC CAGTGGCACA TGGGGCTTTG GCTGTAACTT AACATGCCAG 
TGCCTCAACG GGGGAGCCTG CAACACCCTG GACGGGACCT GCACGTGTGC ACCTGGATGG 
CGCGGGGAGA AATGCGAACT TCCCTGCCAG GATGGCACGT ACGGGCTGAA CTGTGCTGAG 
CGCTGCGACT GCAGCCACGC AGATGGCTGC CACCCTACCA CGGGCCATTG CCGCTGCCTC 
CCCGGATGGT CAGGTGTCCA CTGTGACAGC GTGTGTGCTG AGGGACGCTG GGGCCCCAAC 
TGCTCCCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCTGATGA TGGCATCTGC 
GAGTGTGCAC CAGGCTTCCG AGGCACCACT TGTCAGAGGA TCTGCTCCCC TGGTTTTTAT 
GGGCATCGCT GCAGCCAGAC ATGCCCACAG TGCGTTCACA GCAGCGGGCC CTGCCACCAC 
ATCACCGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGCG CCCTCTGCAA TGAAGTGTGT 
CCCAGTGGCA GATTTGGGAA AAACTGTGCA GGAATTTGTA CCTGCACCAA CAACGGAACC 
TGTAACCCCA TTGACAGATC TTGTCAGTGT TACCCCGGTT GGATTGGCAG TGACTGCTCT 
CAACCATGTC CACCTGCCCA CTGGGGCCCA AACTGCATCC ACACGTGCAA CTGCCATAAT 
GGAGCTTTCT GCAGCGCCTA CGATGGGGAA TGTAAATGCA CTCCTGGCTG GACAGGGCTC 
TACTGCACTC AGAGATGTCC TCTAGGGTTT TATGGAAAAG ATTGTGCACT GATATGCCAA 
TGTCAAAACG GAGCTGACTG CGACCACATT TCTGGGCAGT GTACTTGCCG CACTGGATTC 
ATGGGACGGC ACTGTGAGCA GAAGTGCCCT TCAGGAACAT ATGGCTATGG CTGTCGCCAG 
ATATGTGATT GTCTGAACAA CTCCACCTGC GACCACATCA CTGGGACCTG TTACTGCAGC 
CCCGGATGGA AGGGAGCGAG ATGTGATCAA GCTGGTGTTA TCATAGTTGG AAATCTGAAC 
AGCTTAAGCC GAACCAGTAC TGCTCTCCCT GCTGATTCCT ACCAGATCGG GGCCATTGCA 
GGCATCATCA TTCTTGTCCT AGTTGTTCTC TTCCTACTGG CATTGTTCAT TATTTATAGA 
CACAAGCAGA AGGGAAAGGA ATCAAGCATG CCAGCAGTTA CCTACACCCC TGCTATGAGG 
GTCGTCAATG CAGATTATAC CATTTCAGGA ACCCTTCCTC ACAGCAATGG TGGAAACGCT 
AATAGCCACT ACTTCACCAA TCCCAGTTAC CACACGCTCA CCCAGTGTGC CACATCCCCT 
CACGTCAACA ACAGGGACAG GATGACTGTC ACGAAGTCAA AAAACAATCA ACTGTTTGTG 
AATCTTAAAA ATGTGAACCC TGGGAAGAGA GGCCCTGTGG GGGACTGCAC TGGGACATTG 
CCGGCTGACT GGAAACATGG CGGCTACCTC AACGAGCTCG GTGCTTTTGG ACTTGACAGA 
AGCTATATGG GAAAATCCTT AAAAGACCTG GGAAAGAATT CTGAATATAA TTCAAGTAAC 
TGCTCCCTAA GCAGTTCTGA GAACCCATAT GCCACTATTA AAGACCCACC TGTACTTATC 
CCGAAAAGCT CAGAGTGTGG TTATGTGGAG ATGAAATCGC CGGCACGAAG AGATTCCCCA 
TATGCAGAGA TCAATAACTC AACTTCAGCC AACAGGAATG TCTATGAAGT TGAACCTACA 
GTGAGTGTTG TCCAAGGAGT ATTCAGCAAT AATGGGCGTC TCTCCCAGGA TCCATATGAC 
CTCCCAAAGA ACAGTCACAT CCCTTGTCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA 
TCCTCCCCTA AGCAAGAGGA CAGTGGTGGT AGCAGCAGCA ACAGCAGCAG CAGCAGTGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243474.1
CDS	312..3734
Translation	MVISLNSCLSFICLLLCHWIGTASPLNLEDPNVCSHWESYSVTVQESYPHPFDQIYYTSCTDILNWFKCTRHRVSYRTAYRHGEKTMYRRKSQCCPGFYESGEMCVPHCADKCVHGRCIAPNTCQCEPGWGGTNCSSACDGDHWGPHCTSRCQCKNGALCNPITGACHCAAGFRGWRCEDRCEQGTYGNDCHQRCQCQNGATCDHVTGECRCPPGYTGAFCEDLCPPGKHGPQCEQRCPCQNGGVCHHVTGECSCPSGWMGTVCGQPCPEGRFGKNCSQECQCHNGGTCDAATGQCHCSPGYTGERCQDECPVGTYGVLCAETCQCVNGGKCYHVSGACLCEAGFAGERCEARLCPEGLYGIKCDKRCPCHLENTHSCHPMSGECACKPGWSGLYCNETCSPGFYGEACQQICSCQNGADCDSVTGKCTCAPGFKGIDCSTPCPLGTYGINCSSRCGCKNDAVCSPVDGSCTCKAGWHGVDCSIRCPSGTWGFGCNLTCQCLNGGACNTLDGTCTCAPGWRGEKCELPCQDGTYGLNCAERCDCSHADGCHPTTGHCRCLPGWSGVHCDSVCAEGRWGPNCSLPCYCKNGASCSPDDGICECAPGFRGTTCQRICSPGFYGHRCSQTCPQCVHSSGPCHHITGLCDCLPGFTGALCNEVCPSGRFGKNCAGICTCTNNGTCNPIDRSCQCYPGWIGSDCSQPCPPAHWGPNCIHTCNCHNGAFCSAYDGECKCTPGWTGLYCTQRCPLGFYGKDCALICQCQNGADCDHISGQCTCRTGFMGRHCEQKCPSGTYGYGCRQICDCLNNSTCDHITGTCYCSPGWKGARCDQAGVIIVGNLNSLSRTSTALPADSYQIGAIAGIIILVLVVLFLLALFIIYRHKQKGKESSMPAVTYTPAMRVVNADYTISGTLPHSNGGNANSHYFTNPSYHTLTQCATSPHVNNRDRMTVTKSKNNQLFVNLKNVNPGKRGPVGDCTGTLPADWKHGGYLNELGAFGLDRSYMGKSLKDLGKNSEYNSSNCSLSSSENPYATIKDPPVLIPKSSECGYVEMKSPARRDSPYAEINNSTSANRNVYEVEPTVSVVQGVFSNNGRLSQDPYDLPKNSHIPCHYDLLPVRDSSSSPKQEDSGGSSSNSSSSSE

Target ORF information:

RefSeq Version	NM_001256545.1
Organism	Homo sapiens(human)
Definition	Homo sapiens multiple EGF like domains 10 (MEGF10), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256545.1

ORF Insert Sequence:

ATGGTTATTT CTTTGAACTC ATGCCTGAGC TTTATTTGTT TATTGTTATG CCACTGGATT 
GGGACAGCAT CACCTCTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC 
TCAGTGACTG TGCAAGAGTC ATACCCACAT CCCTTTGATC AAATTTACTA CACGAGCTGC 
ACTGACATTC TAAACTGGTT TAAATGCACG CGGCACAGAG TCAGCTATCG GACAGCCTAT 
CGACATGGGG AGAAGACTAT GTATAGGCGC AAGTCTCAGT GTTGTCCTGG ATTTTATGAA 
AGCGGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGTATTGCT 
CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGAGGGACCA ACTGCTCCAG TGCCTGCGAT 
GGTGATCACT GGGGTCCCCA CTGCACCAGC CGGTGCCAGT GCAAAAATGG GGCTCTGTGC 
AACCCCATCA CCGGGGCTTG CCACTGTGCT GCGGGCTTCC GGGGCTGGCG CTGCGAGGAC 
CGCTGTGAGC AGGGCACCTA TGGTAACGAC TGTCATCAGA GATGCCAGTG CCAGAATGGA 
GCCACCTGCG ACCACGTCAC GGGGGAATGC CGCTGCCCAC CAGGATACAC CGGAGCCTTC 
TGTGAGGATC TTTGTCCTCC TGGTAAACAT GGTCCACAGT GTGAGCAGAG ATGCCCTTGT 
CAAAATGGAG GAGTGTGTCA TCACGTCACT GGAGAATGCT CTTGCCCTTC TGGCTGGATG 
GGCACAGTGT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCCCAAGAA 
TGCCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA 
GGATACACAG GGGAACGGTG CCAGGATGAG TGTCCTGTTG GGACCTATGG CGTTCTCTGT 
GCTGAGACCT GCCAGTGTGT CAACGGAGGG AAGTGTTACC ACGTGAGCGG CGCATGCCTC 
TGTGAAGCAG GCTTTGCTGG CGAGCGCTGC GAAGCACGCC TGTGTCCTGA GGGGCTCTAC 
GGCATCAAAT GTGACAAACG GTGTCCCTGC CACCTGGAAA ACACTCATAG CTGTCACCCC 
ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA TGAGACATGT 
TCTCCTGGAT TCTACGGGGA AGCTTGCCAG CAGATCTGCA GCTGCCAAAA TGGGGCAGAC 
TGTGACAGTG TGACTGGAAA GTGCACCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT 
ACCCCATGCC CTCTGGGAAC CTATGGGATA AACTGTTCCT CTCGCTGTGG CTGTAAAAAT 
GATGCAGTCT GCTCTCCTGT GGACGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG 
GACTGCTCCA TCAGATGTCC CAGTGGCACA TGGGGCTTTG GCTGTAACTT AACATGCCAG 
TGCCTCAACG GGGGAGCCTG CAACACCCTG GACGGGACCT GCACGTGTGC ACCTGGATGG 
CGCGGGGAGA AATGCGAACT TCCCTGCCAG GATGGCACGT ACGGGCTGAA CTGTGCTGAG 
CGCTGCGACT GCAGCCACGC AGATGGCTGC CACCCTACCA CGGGCCATTG CCGCTGCCTC 
CCCGGATGGT CAGGTGTCCA CTGTGACAGC GTGTGTGCTG AGGGACGCTG GGGCCCCAAC 
TGCTCCCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCTGATGA TGGCATCTGC 
GAGTGTGCAC CAGGCTTCCG AGGCACCACT TGTCAGAGGA TCTGCTCCCC TGGTTTTTAT 
GGGCATCGCT GCAGCCAGAC ATGCCCACAG TGCGTTCACA GCAGCGGGCC CTGCCACCAC 
ATCACCGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGCG CCCTCTGCAA TGAAGTGTGT 
CCCAGTGGCA GATTTGGGAA AAACTGTGCA GGAATTTGTA CCTGCACCAA CAACGGAACC 
TGTAACCCCA TTGACAGATC TTGTCAGTGT TACCCCGGTT GGATTGGCAG TGACTGCTCT 
CAACCATGTC CACCTGCCCA CTGGGGCCCA AACTGCATCC ACACGTGCAA CTGCCATAAT 
GGAGCTTTCT GCAGCGCCTA CGATGGGGAA TGTAAATGCA CTCCTGGCTG GACAGGGCTC 
TACTGCACTC AGAGATGTCC TCTAGGGTTT TATGGAAAAG ATTGTGCACT GATATGCCAA 
TGTCAAAACG GAGCTGACTG CGACCACATT TCTGGGCAGT GTACTTGCCG CACTGGATTC 
ATGGGACGGC ACTGTGAGCA GAAGTGCCCT TCAGGAACAT ATGGCTATGG CTGTCGCCAG 
ATATGTGATT GTCTGAACAA CTCCACCTGC GACCACATCA CTGGGACCTG TTACTGCAGC 
CCCGGATGGA AGGGAGCGAG ATGTGATCAA GCTGGTGTTA TCATAGTTGG AAATCTGAAC 
AGCTTAAGCC GAACCAGTAC TGCTCTCCCT GCTGATTCCT ACCAGATCGG GGCCATTGCA 
GGCATCATCA TTCTTGTCCT AGTTGTTCTC TTCCTACTGG CATTGTTCAT TATTTATAGA 
CACAAGCAGA AGGGAAAGGA ATCAAGCATG CCAGCAGTTA CCTACACCCC TGCTATGAGG 
GTCGTCAATG CAGATTATAC CATTTCAGGA ACCCTTCCTC ACAGCAATGG TGGAAACGCT 
AATAGCCACT ACTTCACCAA TCCCAGTTAC CACACGCTCA CCCAGTGTGC CACATCCCCT 
CACGTCAACA ACAGGGACAG GATGACTGTC ACGAAGTCAA AAAACAATCA ACTGTTTGTG 
AATCTTAAAA ATGTGAACCC TGGGAAGAGA GGCCCTGTGG GGGACTGCAC TGGGACATTG 
CCGGCTGACT GGAAACATGG CGGCTACCTC AACGAGCTCG GTGCTTTTGG ACTTGACAGA 
AGCTATATGG GAAAATCCTT AAAAGACCTG GGAAAGAATT CTGAATATAA TTCAAGTAAC 
TGCTCCCTAA GCAGTTCTGA GAACCCATAT GCCACTATTA AAGACCCACC TGTACTTATC 
CCGAAAAGCT CAGAGTGTGG TTATGTGGAG ATGAAATCGC CGGCACGAAG AGATTCCCCA 
TATGCAGAGA TCAATAACTC AACTTCAGCC AACAGGAATG TCTATGAAGT TGAACCTACA 
GTGAGTGTTG TCCAAGGAGT ATTCAGCAAT AATGGGCGTC TCTCCCAGGA TCCATATGAC 
CTCCCAAAGA ACAGTCACAT CCCTTGTCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA 
TCCTCCCCTA AGCAAGAGGA CAGTGGTGGT AGCAGCAGCA ACAGCAGCAG CAGCAGTGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEGF10 ( NM_001256545.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243474.1 Homo sapiens multiple EGF like domains 10 (MEGF10), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu12082
Clone ID Related Accession (Same CDS sequence)	NM_001256545.1 , NM_001256545.2 , NM_032446.3 , XM_011543694.1 , NM_032446.2
Accession Version	NM_001256545.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-11
Organism	Homo sapiens(human)
Product	multiple epidermal growth factor-like domains protein 10 isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA240513.1, AC008402.6, AC008682.6, DA738723.1 and AC010424.9. On Nov 22, 2018 this sequence version replaced NM_001256545.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR749437.1, SRR1660805.16218.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000503335.7/ ENSP00000423354.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTTATTT CTTTGAACTC ATGCCTGAGC TTTATTTGTT TATTGTTATG CCACTGGATT 
GGGACAGCAT CACCTCTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC 
TCAGTGACTG TGCAAGAGTC ATACCCACAT CCCTTTGATC AAATTTACTA CACGAGCTGC 
ACTGACATTC TAAACTGGTT TAAATGCACG CGGCACAGAG TCAGCTATCG GACAGCCTAT 
CGACATGGGG AGAAGACTAT GTATAGGCGC AAGTCTCAGT GTTGTCCTGG ATTTTATGAA 
AGCGGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGTATTGCT 
CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGAGGGACCA ACTGCTCCAG TGCCTGCGAT 
GGTGATCACT GGGGTCCCCA CTGCACCAGC CGGTGCCAGT GCAAAAATGG GGCTCTGTGC 
AACCCCATCA CCGGGGCTTG CCACTGTGCT GCGGGCTTCC GGGGCTGGCG CTGCGAGGAC 
CGCTGTGAGC AGGGCACCTA TGGTAACGAC TGTCATCAGA GATGCCAGTG CCAGAATGGA 
GCCACCTGCG ACCACGTCAC GGGGGAATGC CGCTGCCCAC CAGGATACAC CGGAGCCTTC 
TGTGAGGATC TTTGTCCTCC TGGTAAACAT GGTCCACAGT GTGAGCAGAG ATGCCCTTGT 
CAAAATGGAG GAGTGTGTCA TCACGTCACT GGAGAATGCT CTTGCCCTTC TGGCTGGATG 
GGCACAGTGT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCCCAAGAA 
TGCCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA 
GGATACACAG GGGAACGGTG CCAGGATGAG TGTCCTGTTG GGACCTATGG CGTTCTCTGT 
GCTGAGACCT GCCAGTGTGT CAACGGAGGG AAGTGTTACC ACGTGAGCGG CGCATGCCTC 
TGTGAAGCAG GCTTTGCTGG CGAGCGCTGC GAAGCACGCC TGTGTCCTGA GGGGCTCTAC 
GGCATCAAAT GTGACAAACG GTGTCCCTGC CACCTGGAAA ACACTCATAG CTGTCACCCC 
ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA TGAGACATGT 
TCTCCTGGAT TCTACGGGGA AGCTTGCCAG CAGATCTGCA GCTGCCAAAA TGGGGCAGAC 
TGTGACAGTG TGACTGGAAA GTGCACCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT 
ACCCCATGCC CTCTGGGAAC CTATGGGATA AACTGTTCCT CTCGCTGTGG CTGTAAAAAT 
GATGCAGTCT GCTCTCCTGT GGACGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG 
GACTGCTCCA TCAGATGTCC CAGTGGCACA TGGGGCTTTG GCTGTAACTT AACATGCCAG 
TGCCTCAACG GGGGAGCCTG CAACACCCTG GACGGGACCT GCACGTGTGC ACCTGGATGG 
CGCGGGGAGA AATGCGAACT TCCCTGCCAG GATGGCACGT ACGGGCTGAA CTGTGCTGAG 
CGCTGCGACT GCAGCCACGC AGATGGCTGC CACCCTACCA CGGGCCATTG CCGCTGCCTC 
CCCGGATGGT CAGGTGTCCA CTGTGACAGC GTGTGTGCTG AGGGACGCTG GGGCCCCAAC 
TGCTCCCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCTGATGA TGGCATCTGC 
GAGTGTGCAC CAGGCTTCCG AGGCACCACT TGTCAGAGGA TCTGCTCCCC TGGTTTTTAT 
GGGCATCGCT GCAGCCAGAC ATGCCCACAG TGCGTTCACA GCAGCGGGCC CTGCCACCAC 
ATCACCGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGCG CCCTCTGCAA TGAAGTGTGT 
CCCAGTGGCA GATTTGGGAA AAACTGTGCA GGAATTTGTA CCTGCACCAA CAACGGAACC 
TGTAACCCCA TTGACAGATC TTGTCAGTGT TACCCCGGTT GGATTGGCAG TGACTGCTCT 
CAACCATGTC CACCTGCCCA CTGGGGCCCA AACTGCATCC ACACGTGCAA CTGCCATAAT 
GGAGCTTTCT GCAGCGCCTA CGATGGGGAA TGTAAATGCA CTCCTGGCTG GACAGGGCTC 
TACTGCACTC AGAGATGTCC TCTAGGGTTT TATGGAAAAG ATTGTGCACT GATATGCCAA 
TGTCAAAACG GAGCTGACTG CGACCACATT TCTGGGCAGT GTACTTGCCG CACTGGATTC 
ATGGGACGGC ACTGTGAGCA GAAGTGCCCT TCAGGAACAT ATGGCTATGG CTGTCGCCAG 
ATATGTGATT GTCTGAACAA CTCCACCTGC GACCACATCA CTGGGACCTG TTACTGCAGC 
CCCGGATGGA AGGGAGCGAG ATGTGATCAA GCTGGTGTTA TCATAGTTGG AAATCTGAAC 
AGCTTAAGCC GAACCAGTAC TGCTCTCCCT GCTGATTCCT ACCAGATCGG GGCCATTGCA 
GGCATCATCA TTCTTGTCCT AGTTGTTCTC TTCCTACTGG CATTGTTCAT TATTTATAGA 
CACAAGCAGA AGGGAAAGGA ATCAAGCATG CCAGCAGTTA CCTACACCCC TGCTATGAGG 
GTCGTCAATG CAGATTATAC CATTTCAGGA ACCCTTCCTC ACAGCAATGG TGGAAACGCT 
AATAGCCACT ACTTCACCAA TCCCAGTTAC CACACGCTCA CCCAGTGTGC CACATCCCCT 
CACGTCAACA ACAGGGACAG GATGACTGTC ACGAAGTCAA AAAACAATCA ACTGTTTGTG 
AATCTTAAAA ATGTGAACCC TGGGAAGAGA GGCCCTGTGG GGGACTGCAC TGGGACATTG 
CCGGCTGACT GGAAACATGG CGGCTACCTC AACGAGCTCG GTGCTTTTGG ACTTGACAGA 
AGCTATATGG GAAAATCCTT AAAAGACCTG GGAAAGAATT CTGAATATAA TTCAAGTAAC 
TGCTCCCTAA GCAGTTCTGA GAACCCATAT GCCACTATTA AAGACCCACC TGTACTTATC 
CCGAAAAGCT CAGAGTGTGG TTATGTGGAG ATGAAATCGC CGGCACGAAG AGATTCCCCA 
TATGCAGAGA TCAATAACTC AACTTCAGCC AACAGGAATG TCTATGAAGT TGAACCTACA 
GTGAGTGTTG TCCAAGGAGT ATTCAGCAAT AATGGGCGTC TCTCCCAGGA TCCATATGAC 
CTCCCAAAGA ACAGTCACAT CCCTTGTCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA 
TCCTCCCCTA AGCAAGAGGA CAGTGGTGGT AGCAGCAGCA ACAGCAGCAG CAGCAGTGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243474.1
CDS	280..3702
Translation	MVISLNSCLSFICLLLCHWIGTASPLNLEDPNVCSHWESYSVTVQESYPHPFDQIYYTSCTDILNWFKCTRHRVSYRTAYRHGEKTMYRRKSQCCPGFYESGEMCVPHCADKCVHGRCIAPNTCQCEPGWGGTNCSSACDGDHWGPHCTSRCQCKNGALCNPITGACHCAAGFRGWRCEDRCEQGTYGNDCHQRCQCQNGATCDHVTGECRCPPGYTGAFCEDLCPPGKHGPQCEQRCPCQNGGVCHHVTGECSCPSGWMGTVCGQPCPEGRFGKNCSQECQCHNGGTCDAATGQCHCSPGYTGERCQDECPVGTYGVLCAETCQCVNGGKCYHVSGACLCEAGFAGERCEARLCPEGLYGIKCDKRCPCHLENTHSCHPMSGECACKPGWSGLYCNETCSPGFYGEACQQICSCQNGADCDSVTGKCTCAPGFKGIDCSTPCPLGTYGINCSSRCGCKNDAVCSPVDGSCTCKAGWHGVDCSIRCPSGTWGFGCNLTCQCLNGGACNTLDGTCTCAPGWRGEKCELPCQDGTYGLNCAERCDCSHADGCHPTTGHCRCLPGWSGVHCDSVCAEGRWGPNCSLPCYCKNGASCSPDDGICECAPGFRGTTCQRICSPGFYGHRCSQTCPQCVHSSGPCHHITGLCDCLPGFTGALCNEVCPSGRFGKNCAGICTCTNNGTCNPIDRSCQCYPGWIGSDCSQPCPPAHWGPNCIHTCNCHNGAFCSAYDGECKCTPGWTGLYCTQRCPLGFYGKDCALICQCQNGADCDHISGQCTCRTGFMGRHCEQKCPSGTYGYGCRQICDCLNNSTCDHITGTCYCSPGWKGARCDQAGVIIVGNLNSLSRTSTALPADSYQIGAIAGIIILVLVVLFLLALFIIYRHKQKGKESSMPAVTYTPAMRVVNADYTISGTLPHSNGGNANSHYFTNPSYHTLTQCATSPHVNNRDRMTVTKSKNNQLFVNLKNVNPGKRGPVGDCTGTLPADWKHGGYLNELGAFGLDRSYMGKSLKDLGKNSEYNSSNCSLSSSENPYATIKDPPVLIPKSSECGYVEMKSPARRDSPYAEINNSTSANRNVYEVEPTVSVVQGVFSNNGRLSQDPYDLPKNSHIPCHYDLLPVRDSSSSPKQEDSGGSSSNSSSSSE

Target ORF information:

RefSeq Version	NM_001256545.2
Organism	Homo sapiens(human)
Definition	Homo sapiens multiple EGF like domains 10 (MEGF10), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256545.2

ORF Insert Sequence:

ATGGTTATTT CTTTGAACTC ATGCCTGAGC TTTATTTGTT TATTGTTATG CCACTGGATT 
GGGACAGCAT CACCTCTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC 
TCAGTGACTG TGCAAGAGTC ATACCCACAT CCCTTTGATC AAATTTACTA CACGAGCTGC 
ACTGACATTC TAAACTGGTT TAAATGCACG CGGCACAGAG TCAGCTATCG GACAGCCTAT 
CGACATGGGG AGAAGACTAT GTATAGGCGC AAGTCTCAGT GTTGTCCTGG ATTTTATGAA 
AGCGGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGTATTGCT 
CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGAGGGACCA ACTGCTCCAG TGCCTGCGAT 
GGTGATCACT GGGGTCCCCA CTGCACCAGC CGGTGCCAGT GCAAAAATGG GGCTCTGTGC 
AACCCCATCA CCGGGGCTTG CCACTGTGCT GCGGGCTTCC GGGGCTGGCG CTGCGAGGAC 
CGCTGTGAGC AGGGCACCTA TGGTAACGAC TGTCATCAGA GATGCCAGTG CCAGAATGGA 
GCCACCTGCG ACCACGTCAC GGGGGAATGC CGCTGCCCAC CAGGATACAC CGGAGCCTTC 
TGTGAGGATC TTTGTCCTCC TGGTAAACAT GGTCCACAGT GTGAGCAGAG ATGCCCTTGT 
CAAAATGGAG GAGTGTGTCA TCACGTCACT GGAGAATGCT CTTGCCCTTC TGGCTGGATG 
GGCACAGTGT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCCCAAGAA 
TGCCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA 
GGATACACAG GGGAACGGTG CCAGGATGAG TGTCCTGTTG GGACCTATGG CGTTCTCTGT 
GCTGAGACCT GCCAGTGTGT CAACGGAGGG AAGTGTTACC ACGTGAGCGG CGCATGCCTC 
TGTGAAGCAG GCTTTGCTGG CGAGCGCTGC GAAGCACGCC TGTGTCCTGA GGGGCTCTAC 
GGCATCAAAT GTGACAAACG GTGTCCCTGC CACCTGGAAA ACACTCATAG CTGTCACCCC 
ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA TGAGACATGT 
TCTCCTGGAT TCTACGGGGA AGCTTGCCAG CAGATCTGCA GCTGCCAAAA TGGGGCAGAC 
TGTGACAGTG TGACTGGAAA GTGCACCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT 
ACCCCATGCC CTCTGGGAAC CTATGGGATA AACTGTTCCT CTCGCTGTGG CTGTAAAAAT 
GATGCAGTCT GCTCTCCTGT GGACGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG 
GACTGCTCCA TCAGATGTCC CAGTGGCACA TGGGGCTTTG GCTGTAACTT AACATGCCAG 
TGCCTCAACG GGGGAGCCTG CAACACCCTG GACGGGACCT GCACGTGTGC ACCTGGATGG 
CGCGGGGAGA AATGCGAACT TCCCTGCCAG GATGGCACGT ACGGGCTGAA CTGTGCTGAG 
CGCTGCGACT GCAGCCACGC AGATGGCTGC CACCCTACCA CGGGCCATTG CCGCTGCCTC 
CCCGGATGGT CAGGTGTCCA CTGTGACAGC GTGTGTGCTG AGGGACGCTG GGGCCCCAAC 
TGCTCCCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCTGATGA TGGCATCTGC 
GAGTGTGCAC CAGGCTTCCG AGGCACCACT TGTCAGAGGA TCTGCTCCCC TGGTTTTTAT 
GGGCATCGCT GCAGCCAGAC ATGCCCACAG TGCGTTCACA GCAGCGGGCC CTGCCACCAC 
ATCACCGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGCG CCCTCTGCAA TGAAGTGTGT 
CCCAGTGGCA GATTTGGGAA AAACTGTGCA GGAATTTGTA CCTGCACCAA CAACGGAACC 
TGTAACCCCA TTGACAGATC TTGTCAGTGT TACCCCGGTT GGATTGGCAG TGACTGCTCT 
CAACCATGTC CACCTGCCCA CTGGGGCCCA AACTGCATCC ACACGTGCAA CTGCCATAAT 
GGAGCTTTCT GCAGCGCCTA CGATGGGGAA TGTAAATGCA CTCCTGGCTG GACAGGGCTC 
TACTGCACTC AGAGATGTCC TCTAGGGTTT TATGGAAAAG ATTGTGCACT GATATGCCAA 
TGTCAAAACG GAGCTGACTG CGACCACATT TCTGGGCAGT GTACTTGCCG CACTGGATTC 
ATGGGACGGC ACTGTGAGCA GAAGTGCCCT TCAGGAACAT ATGGCTATGG CTGTCGCCAG 
ATATGTGATT GTCTGAACAA CTCCACCTGC GACCACATCA CTGGGACCTG TTACTGCAGC 
CCCGGATGGA AGGGAGCGAG ATGTGATCAA GCTGGTGTTA TCATAGTTGG AAATCTGAAC 
AGCTTAAGCC GAACCAGTAC TGCTCTCCCT GCTGATTCCT ACCAGATCGG GGCCATTGCA 
GGCATCATCA TTCTTGTCCT AGTTGTTCTC TTCCTACTGG CATTGTTCAT TATTTATAGA 
CACAAGCAGA AGGGAAAGGA ATCAAGCATG CCAGCAGTTA CCTACACCCC TGCTATGAGG 
GTCGTCAATG CAGATTATAC CATTTCAGGA ACCCTTCCTC ACAGCAATGG TGGAAACGCT 
AATAGCCACT ACTTCACCAA TCCCAGTTAC CACACGCTCA CCCAGTGTGC CACATCCCCT 
CACGTCAACA ACAGGGACAG GATGACTGTC ACGAAGTCAA AAAACAATCA ACTGTTTGTG 
AATCTTAAAA ATGTGAACCC TGGGAAGAGA GGCCCTGTGG GGGACTGCAC TGGGACATTG 
CCGGCTGACT GGAAACATGG CGGCTACCTC AACGAGCTCG GTGCTTTTGG ACTTGACAGA 
AGCTATATGG GAAAATCCTT AAAAGACCTG GGAAAGAATT CTGAATATAA TTCAAGTAAC 
TGCTCCCTAA GCAGTTCTGA GAACCCATAT GCCACTATTA AAGACCCACC TGTACTTATC 
CCGAAAAGCT CAGAGTGTGG TTATGTGGAG ATGAAATCGC CGGCACGAAG AGATTCCCCA 
TATGCAGAGA TCAATAACTC AACTTCAGCC AACAGGAATG TCTATGAAGT TGAACCTACA 
GTGAGTGTTG TCCAAGGAGT ATTCAGCAAT AATGGGCGTC TCTCCCAGGA TCCATATGAC 
CTCCCAAAGA ACAGTCACAT CCCTTGTCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA 
TCCTCCCCTA AGCAAGAGGA CAGTGGTGGT AGCAGCAGCA ACAGCAGCAG CAGCAGTGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEGF10 ( NM_032446.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_115822.1 Homo sapiens multiple EGF like domains 10 (MEGF10), transcript variant 1, mRNA.

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CloneID	OHu12082
Clone ID Related Accession (Same CDS sequence)	NM_001256545.1 , NM_001256545.2 , NM_032446.3 , XM_011543694.1 , NM_032446.2
Accession Version	NM_032446.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-11
Organism	Homo sapiens(human)
Product	multiple epidermal growth factor-like domains protein 10 isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA240513.1, AB058676.1, AC008682.6 and AC010424.9. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_032446.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Both variants 1 and 2 encode isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC152478.1, AB058676.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTTATTT CTTTGAACTC ATGCCTGAGC TTTATTTGTT TATTGTTATG CCACTGGATT 
GGGACAGCAT CACCTCTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC 
TCAGTGACTG TGCAAGAGTC ATACCCACAT CCCTTTGATC AAATTTACTA CACGAGCTGC 
ACTGACATTC TAAACTGGTT TAAATGCACG CGGCACAGAG TCAGCTATCG GACAGCCTAT 
CGACATGGGG AGAAGACTAT GTATAGGCGC AAGTCTCAGT GTTGTCCTGG ATTTTATGAA 
AGCGGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGTATTGCT 
CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGAGGGACCA ACTGCTCCAG TGCCTGCGAT 
GGTGATCACT GGGGTCCCCA CTGCACCAGC CGGTGCCAGT GCAAAAATGG GGCTCTGTGC 
AACCCCATCA CCGGGGCTTG CCACTGTGCT GCGGGCTTCC GGGGCTGGCG CTGCGAGGAC 
CGCTGTGAGC AGGGCACCTA TGGTAACGAC TGTCATCAGA GATGCCAGTG CCAGAATGGA 
GCCACCTGCG ACCACGTCAC GGGGGAATGC CGCTGCCCAC CAGGATACAC CGGAGCCTTC 
TGTGAGGATC TTTGTCCTCC TGGTAAACAT GGTCCACAGT GTGAGCAGAG ATGCCCTTGT 
CAAAATGGAG GAGTGTGTCA TCACGTCACT GGAGAATGCT CTTGCCCTTC TGGCTGGATG 
GGCACAGTGT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCCCAAGAA 
TGCCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA 
GGATACACAG GGGAACGGTG CCAGGATGAG TGTCCTGTTG GGACCTATGG CGTTCTCTGT 
GCTGAGACCT GCCAGTGTGT CAACGGAGGG AAGTGTTACC ACGTGAGCGG CGCATGCCTC 
TGTGAAGCAG GCTTTGCTGG CGAGCGCTGC GAAGCACGCC TGTGTCCTGA GGGGCTCTAC 
GGCATCAAAT GTGACAAACG GTGTCCCTGC CACCTGGAAA ACACTCATAG CTGTCACCCC 
ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA TGAGACATGT 
TCTCCTGGAT TCTACGGGGA AGCTTGCCAG CAGATCTGCA GCTGCCAAAA TGGGGCAGAC 
TGTGACAGTG TGACTGGAAA GTGCACCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT 
ACCCCATGCC CTCTGGGAAC CTATGGGATA AACTGTTCCT CTCGCTGTGG CTGTAAAAAT 
GATGCAGTCT GCTCTCCTGT GGACGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG 
GACTGCTCCA TCAGATGTCC CAGTGGCACA TGGGGCTTTG GCTGTAACTT AACATGCCAG 
TGCCTCAACG GGGGAGCCTG CAACACCCTG GACGGGACCT GCACGTGTGC ACCTGGATGG 
CGCGGGGAGA AATGCGAACT TCCCTGCCAG GATGGCACGT ACGGGCTGAA CTGTGCTGAG 
CGCTGCGACT GCAGCCACGC AGATGGCTGC CACCCTACCA CGGGCCATTG CCGCTGCCTC 
CCCGGATGGT CAGGTGTCCA CTGTGACAGC GTGTGTGCTG AGGGACGCTG GGGCCCCAAC 
TGCTCCCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCTGATGA TGGCATCTGC 
GAGTGTGCAC CAGGCTTCCG AGGCACCACT TGTCAGAGGA TCTGCTCCCC TGGTTTTTAT 
GGGCATCGCT GCAGCCAGAC ATGCCCACAG TGCGTTCACA GCAGCGGGCC CTGCCACCAC 
ATCACCGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGCG CCCTCTGCAA TGAAGTGTGT 
CCCAGTGGCA GATTTGGGAA AAACTGTGCA GGAATTTGTA CCTGCACCAA CAACGGAACC 
TGTAACCCCA TTGACAGATC TTGTCAGTGT TACCCCGGTT GGATTGGCAG TGACTGCTCT 
CAACCATGTC CACCTGCCCA CTGGGGCCCA AACTGCATCC ACACGTGCAA CTGCCATAAT 
GGAGCTTTCT GCAGCGCCTA CGATGGGGAA TGTAAATGCA CTCCTGGCTG GACAGGGCTC 
TACTGCACTC AGAGATGTCC TCTAGGGTTT TATGGAAAAG ATTGTGCACT GATATGCCAA 
TGTCAAAACG GAGCTGACTG CGACCACATT TCTGGGCAGT GTACTTGCCG CACTGGATTC 
ATGGGACGGC ACTGTGAGCA GAAGTGCCCT TCAGGAACAT ATGGCTATGG CTGTCGCCAG 
ATATGTGATT GTCTGAACAA CTCCACCTGC GACCACATCA CTGGGACCTG TTACTGCAGC 
CCCGGATGGA AGGGAGCGAG ATGTGATCAA GCTGGTGTTA TCATAGTTGG AAATCTGAAC 
AGCTTAAGCC GAACCAGTAC TGCTCTCCCT GCTGATTCCT ACCAGATCGG GGCCATTGCA 
GGCATCATCA TTCTTGTCCT AGTTGTTCTC TTCCTACTGG CATTGTTCAT TATTTATAGA 
CACAAGCAGA AGGGAAAGGA ATCAAGCATG CCAGCAGTTA CCTACACCCC TGCTATGAGG 
GTCGTCAATG CAGATTATAC CATTTCAGGA ACCCTTCCTC ACAGCAATGG TGGAAACGCT 
AATAGCCACT ACTTCACCAA TCCCAGTTAC CACACGCTCA CCCAGTGTGC CACATCCCCT 
CACGTCAACA ACAGGGACAG GATGACTGTC ACGAAGTCAA AAAACAATCA ACTGTTTGTG 
AATCTTAAAA ATGTGAACCC TGGGAAGAGA GGCCCTGTGG GGGACTGCAC TGGGACATTG 
CCGGCTGACT GGAAACATGG CGGCTACCTC AACGAGCTCG GTGCTTTTGG ACTTGACAGA 
AGCTATATGG GAAAATCCTT AAAAGACCTG GGAAAGAATT CTGAATATAA TTCAAGTAAC 
TGCTCCCTAA GCAGTTCTGA GAACCCATAT GCCACTATTA AAGACCCACC TGTACTTATC 
CCGAAAAGCT CAGAGTGTGG TTATGTGGAG ATGAAATCGC CGGCACGAAG AGATTCCCCA 
TATGCAGAGA TCAATAACTC AACTTCAGCC AACAGGAATG TCTATGAAGT TGAACCTACA 
GTGAGTGTTG TCCAAGGAGT ATTCAGCAAT AATGGGCGTC TCTCCCAGGA TCCATATGAC 
CTCCCAAAGA ACAGTCACAT CCCTTGTCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA 
TCCTCCCCTA AGCAAGAGGA CAGTGGTGGT AGCAGCAGCA ACAGCAGCAG CAGCAGTGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115822.1
CDS	331..3753
Translation	MVISLNSCLSFICLLLCHWIGTASPLNLEDPNVCSHWESYSVTVQESYPHPFDQIYYTSCTDILNWFKCTRHRVSYRTAYRHGEKTMYRRKSQCCPGFYESGEMCVPHCADKCVHGRCIAPNTCQCEPGWGGTNCSSACDGDHWGPHCTSRCQCKNGALCNPITGACHCAAGFRGWRCEDRCEQGTYGNDCHQRCQCQNGATCDHVTGECRCPPGYTGAFCEDLCPPGKHGPQCEQRCPCQNGGVCHHVTGECSCPSGWMGTVCGQPCPEGRFGKNCSQECQCHNGGTCDAATGQCHCSPGYTGERCQDECPVGTYGVLCAETCQCVNGGKCYHVSGACLCEAGFAGERCEARLCPEGLYGIKCDKRCPCHLENTHSCHPMSGECACKPGWSGLYCNETCSPGFYGEACQQICSCQNGADCDSVTGKCTCAPGFKGIDCSTPCPLGTYGINCSSRCGCKNDAVCSPVDGSCTCKAGWHGVDCSIRCPSGTWGFGCNLTCQCLNGGACNTLDGTCTCAPGWRGEKCELPCQDGTYGLNCAERCDCSHADGCHPTTGHCRCLPGWSGVHCDSVCAEGRWGPNCSLPCYCKNGASCSPDDGICECAPGFRGTTCQRICSPGFYGHRCSQTCPQCVHSSGPCHHITGLCDCLPGFTGALCNEVCPSGRFGKNCAGICTCTNNGTCNPIDRSCQCYPGWIGSDCSQPCPPAHWGPNCIHTCNCHNGAFCSAYDGECKCTPGWTGLYCTQRCPLGFYGKDCALICQCQNGADCDHISGQCTCRTGFMGRHCEQKCPSGTYGYGCRQICDCLNNSTCDHITGTCYCSPGWKGARCDQAGVIIVGNLNSLSRTSTALPADSYQIGAIAGIIILVLVVLFLLALFIIYRHKQKGKESSMPAVTYTPAMRVVNADYTISGTLPHSNGGNANSHYFTNPSYHTLTQCATSPHVNNRDRMTVTKSKNNQLFVNLKNVNPGKRGPVGDCTGTLPADWKHGGYLNELGAFGLDRSYMGKSLKDLGKNSEYNSSNCSLSSSENPYATIKDPPVLIPKSSECGYVEMKSPARRDSPYAEINNSTSANRNVYEVEPTVSVVQGVFSNNGRLSQDPYDLPKNSHIPCHYDLLPVRDSSSSPKQEDSGGSSSNSSSSSE

Target ORF information:

RefSeq Version	NM_032446.3
Organism	Homo sapiens(human)
Definition	Homo sapiens multiple EGF like domains 10 (MEGF10), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032446.3

ORF Insert Sequence:

ATGGTTATTT CTTTGAACTC ATGCCTGAGC TTTATTTGTT TATTGTTATG CCACTGGATT 
GGGACAGCAT CACCTCTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC 
TCAGTGACTG TGCAAGAGTC ATACCCACAT CCCTTTGATC AAATTTACTA CACGAGCTGC 
ACTGACATTC TAAACTGGTT TAAATGCACG CGGCACAGAG TCAGCTATCG GACAGCCTAT 
CGACATGGGG AGAAGACTAT GTATAGGCGC AAGTCTCAGT GTTGTCCTGG ATTTTATGAA 
AGCGGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGTATTGCT 
CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGAGGGACCA ACTGCTCCAG TGCCTGCGAT 
GGTGATCACT GGGGTCCCCA CTGCACCAGC CGGTGCCAGT GCAAAAATGG GGCTCTGTGC 
AACCCCATCA CCGGGGCTTG CCACTGTGCT GCGGGCTTCC GGGGCTGGCG CTGCGAGGAC 
CGCTGTGAGC AGGGCACCTA TGGTAACGAC TGTCATCAGA GATGCCAGTG CCAGAATGGA 
GCCACCTGCG ACCACGTCAC GGGGGAATGC CGCTGCCCAC CAGGATACAC CGGAGCCTTC 
TGTGAGGATC TTTGTCCTCC TGGTAAACAT GGTCCACAGT GTGAGCAGAG ATGCCCTTGT 
CAAAATGGAG GAGTGTGTCA TCACGTCACT GGAGAATGCT CTTGCCCTTC TGGCTGGATG 
GGCACAGTGT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCCCAAGAA 
TGCCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA 
GGATACACAG GGGAACGGTG CCAGGATGAG TGTCCTGTTG GGACCTATGG CGTTCTCTGT 
GCTGAGACCT GCCAGTGTGT CAACGGAGGG AAGTGTTACC ACGTGAGCGG CGCATGCCTC 
TGTGAAGCAG GCTTTGCTGG CGAGCGCTGC GAAGCACGCC TGTGTCCTGA GGGGCTCTAC 
GGCATCAAAT GTGACAAACG GTGTCCCTGC CACCTGGAAA ACACTCATAG CTGTCACCCC 
ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA TGAGACATGT 
TCTCCTGGAT TCTACGGGGA AGCTTGCCAG CAGATCTGCA GCTGCCAAAA TGGGGCAGAC 
TGTGACAGTG TGACTGGAAA GTGCACCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT 
ACCCCATGCC CTCTGGGAAC CTATGGGATA AACTGTTCCT CTCGCTGTGG CTGTAAAAAT 
GATGCAGTCT GCTCTCCTGT GGACGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG 
GACTGCTCCA TCAGATGTCC CAGTGGCACA TGGGGCTTTG GCTGTAACTT AACATGCCAG 
TGCCTCAACG GGGGAGCCTG CAACACCCTG GACGGGACCT GCACGTGTGC ACCTGGATGG 
CGCGGGGAGA AATGCGAACT TCCCTGCCAG GATGGCACGT ACGGGCTGAA CTGTGCTGAG 
CGCTGCGACT GCAGCCACGC AGATGGCTGC CACCCTACCA CGGGCCATTG CCGCTGCCTC 
CCCGGATGGT CAGGTGTCCA CTGTGACAGC GTGTGTGCTG AGGGACGCTG GGGCCCCAAC 
TGCTCCCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCTGATGA TGGCATCTGC 
GAGTGTGCAC CAGGCTTCCG AGGCACCACT TGTCAGAGGA TCTGCTCCCC TGGTTTTTAT 
GGGCATCGCT GCAGCCAGAC ATGCCCACAG TGCGTTCACA GCAGCGGGCC CTGCCACCAC 
ATCACCGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGCG CCCTCTGCAA TGAAGTGTGT 
CCCAGTGGCA GATTTGGGAA AAACTGTGCA GGAATTTGTA CCTGCACCAA CAACGGAACC 
TGTAACCCCA TTGACAGATC TTGTCAGTGT TACCCCGGTT GGATTGGCAG TGACTGCTCT 
CAACCATGTC CACCTGCCCA CTGGGGCCCA AACTGCATCC ACACGTGCAA CTGCCATAAT 
GGAGCTTTCT GCAGCGCCTA CGATGGGGAA TGTAAATGCA CTCCTGGCTG GACAGGGCTC 
TACTGCACTC AGAGATGTCC TCTAGGGTTT TATGGAAAAG ATTGTGCACT GATATGCCAA 
TGTCAAAACG GAGCTGACTG CGACCACATT TCTGGGCAGT GTACTTGCCG CACTGGATTC 
ATGGGACGGC ACTGTGAGCA GAAGTGCCCT TCAGGAACAT ATGGCTATGG CTGTCGCCAG 
ATATGTGATT GTCTGAACAA CTCCACCTGC GACCACATCA CTGGGACCTG TTACTGCAGC 
CCCGGATGGA AGGGAGCGAG ATGTGATCAA GCTGGTGTTA TCATAGTTGG AAATCTGAAC 
AGCTTAAGCC GAACCAGTAC TGCTCTCCCT GCTGATTCCT ACCAGATCGG GGCCATTGCA 
GGCATCATCA TTCTTGTCCT AGTTGTTCTC TTCCTACTGG CATTGTTCAT TATTTATAGA 
CACAAGCAGA AGGGAAAGGA ATCAAGCATG CCAGCAGTTA CCTACACCCC TGCTATGAGG 
GTCGTCAATG CAGATTATAC CATTTCAGGA ACCCTTCCTC ACAGCAATGG TGGAAACGCT 
AATAGCCACT ACTTCACCAA TCCCAGTTAC CACACGCTCA CCCAGTGTGC CACATCCCCT 
CACGTCAACA ACAGGGACAG GATGACTGTC ACGAAGTCAA AAAACAATCA ACTGTTTGTG 
AATCTTAAAA ATGTGAACCC TGGGAAGAGA GGCCCTGTGG GGGACTGCAC TGGGACATTG 
CCGGCTGACT GGAAACATGG CGGCTACCTC AACGAGCTCG GTGCTTTTGG ACTTGACAGA 
AGCTATATGG GAAAATCCTT AAAAGACCTG GGAAAGAATT CTGAATATAA TTCAAGTAAC 
TGCTCCCTAA GCAGTTCTGA GAACCCATAT GCCACTATTA AAGACCCACC TGTACTTATC 
CCGAAAAGCT CAGAGTGTGG TTATGTGGAG ATGAAATCGC CGGCACGAAG AGATTCCCCA 
TATGCAGAGA TCAATAACTC AACTTCAGCC AACAGGAATG TCTATGAAGT TGAACCTACA 
GTGAGTGTTG TCCAAGGAGT ATTCAGCAAT AATGGGCGTC TCTCCCAGGA TCCATATGAC 
CTCCCAAAGA ACAGTCACAT CCCTTGTCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA 
TCCTCCCCTA AGCAAGAGGA CAGTGGTGGT AGCAGCAGCA ACAGCAGCAG CAGCAGTGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEGF10 ( NM_032446.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_115822.1 Homo sapiens multiple EGF like domains 10 (MEGF10), transcript variant 1, mRNA.

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CloneID	OHu12082
Clone ID Related Accession (Same CDS sequence)	NM_001256545.1 , NM_001256545.2 , NM_032446.3 , XM_011543694.1 , NM_032446.2
Accession Version	NM_032446.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Homo sapiens(human)
Product	multiple epidermal growth factor-like domains protein 10 isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA240513.1, AB058676.1, AC008682.6 and AC010424.9. This sequence is a reference standard in the RefSeqGene project. On Apr 10, 2008 this sequence version replaced NM_032446.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (a). Both variants 1 and 2 encode isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC152478.1, AB058676.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTTATTT CTTTGAACTC ATGCCTGAGC TTTATTTGTT TATTGTTATG CCACTGGATT 
GGGACAGCAT CACCTCTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC 
TCAGTGACTG TGCAAGAGTC ATACCCACAT CCCTTTGATC AAATTTACTA CACGAGCTGC 
ACTGACATTC TAAACTGGTT TAAATGCACG CGGCACAGAG TCAGCTATCG GACAGCCTAT 
CGACATGGGG AGAAGACTAT GTATAGGCGC AAGTCTCAGT GTTGTCCTGG ATTTTATGAA 
AGCGGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGTATTGCT 
CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGAGGGACCA ACTGCTCCAG TGCCTGCGAT 
GGTGATCACT GGGGTCCCCA CTGCACCAGC CGGTGCCAGT GCAAAAATGG GGCTCTGTGC 
AACCCCATCA CCGGGGCTTG CCACTGTGCT GCGGGCTTCC GGGGCTGGCG CTGCGAGGAC 
CGCTGTGAGC AGGGCACCTA TGGTAACGAC TGTCATCAGA GATGCCAGTG CCAGAATGGA 
GCCACCTGCG ACCACGTCAC GGGGGAATGC CGCTGCCCAC CAGGATACAC CGGAGCCTTC 
TGTGAGGATC TTTGTCCTCC TGGTAAACAT GGTCCACAGT GTGAGCAGAG ATGCCCTTGT 
CAAAATGGAG GAGTGTGTCA TCACGTCACT GGAGAATGCT CTTGCCCTTC TGGCTGGATG 
GGCACAGTGT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCCCAAGAA 
TGCCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA 
GGATACACAG GGGAACGGTG CCAGGATGAG TGTCCTGTTG GGACCTATGG CGTTCTCTGT 
GCTGAGACCT GCCAGTGTGT CAACGGAGGG AAGTGTTACC ACGTGAGCGG CGCATGCCTC 
TGTGAAGCAG GCTTTGCTGG CGAGCGCTGC GAAGCACGCC TGTGTCCTGA GGGGCTCTAC 
GGCATCAAAT GTGACAAACG GTGTCCCTGC CACCTGGAAA ACACTCATAG CTGTCACCCC 
ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA TGAGACATGT 
TCTCCTGGAT TCTACGGGGA AGCTTGCCAG CAGATCTGCA GCTGCCAAAA TGGGGCAGAC 
TGTGACAGTG TGACTGGAAA GTGCACCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT 
ACCCCATGCC CTCTGGGAAC CTATGGGATA AACTGTTCCT CTCGCTGTGG CTGTAAAAAT 
GATGCAGTCT GCTCTCCTGT GGACGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG 
GACTGCTCCA TCAGATGTCC CAGTGGCACA TGGGGCTTTG GCTGTAACTT AACATGCCAG 
TGCCTCAACG GGGGAGCCTG CAACACCCTG GACGGGACCT GCACGTGTGC ACCTGGATGG 
CGCGGGGAGA AATGCGAACT TCCCTGCCAG GATGGCACGT ACGGGCTGAA CTGTGCTGAG 
CGCTGCGACT GCAGCCACGC AGATGGCTGC CACCCTACCA CGGGCCATTG CCGCTGCCTC 
CCCGGATGGT CAGGTGTCCA CTGTGACAGC GTGTGTGCTG AGGGACGCTG GGGCCCCAAC 
TGCTCCCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCTGATGA TGGCATCTGC 
GAGTGTGCAC CAGGCTTCCG AGGCACCACT TGTCAGAGGA TCTGCTCCCC TGGTTTTTAT 
GGGCATCGCT GCAGCCAGAC ATGCCCACAG TGCGTTCACA GCAGCGGGCC CTGCCACCAC 
ATCACCGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGCG CCCTCTGCAA TGAAGTGTGT 
CCCAGTGGCA GATTTGGGAA AAACTGTGCA GGAATTTGTA CCTGCACCAA CAACGGAACC 
TGTAACCCCA TTGACAGATC TTGTCAGTGT TACCCCGGTT GGATTGGCAG TGACTGCTCT 
CAACCATGTC CACCTGCCCA CTGGGGCCCA AACTGCATCC ACACGTGCAA CTGCCATAAT 
GGAGCTTTCT GCAGCGCCTA CGATGGGGAA TGTAAATGCA CTCCTGGCTG GACAGGGCTC 
TACTGCACTC AGAGATGTCC TCTAGGGTTT TATGGAAAAG ATTGTGCACT GATATGCCAA 
TGTCAAAACG GAGCTGACTG CGACCACATT TCTGGGCAGT GTACTTGCCG CACTGGATTC 
ATGGGACGGC ACTGTGAGCA GAAGTGCCCT TCAGGAACAT ATGGCTATGG CTGTCGCCAG 
ATATGTGATT GTCTGAACAA CTCCACCTGC GACCACATCA CTGGGACCTG TTACTGCAGC 
CCCGGATGGA AGGGAGCGAG ATGTGATCAA GCTGGTGTTA TCATAGTTGG AAATCTGAAC 
AGCTTAAGCC GAACCAGTAC TGCTCTCCCT GCTGATTCCT ACCAGATCGG GGCCATTGCA 
GGCATCATCA TTCTTGTCCT AGTTGTTCTC TTCCTACTGG CATTGTTCAT TATTTATAGA 
CACAAGCAGA AGGGAAAGGA ATCAAGCATG CCAGCAGTTA CCTACACCCC TGCTATGAGG 
GTCGTCAATG CAGATTATAC CATTTCAGGA ACCCTTCCTC ACAGCAATGG TGGAAACGCT 
AATAGCCACT ACTTCACCAA TCCCAGTTAC CACACGCTCA CCCAGTGTGC CACATCCCCT 
CACGTCAACA ACAGGGACAG GATGACTGTC ACGAAGTCAA AAAACAATCA ACTGTTTGTG 
AATCTTAAAA ATGTGAACCC TGGGAAGAGA GGCCCTGTGG GGGACTGCAC TGGGACATTG 
CCGGCTGACT GGAAACATGG CGGCTACCTC AACGAGCTCG GTGCTTTTGG ACTTGACAGA 
AGCTATATGG GAAAATCCTT AAAAGACCTG GGAAAGAATT CTGAATATAA TTCAAGTAAC 
TGCTCCCTAA GCAGTTCTGA GAACCCATAT GCCACTATTA AAGACCCACC TGTACTTATC 
CCGAAAAGCT CAGAGTGTGG TTATGTGGAG ATGAAATCGC CGGCACGAAG AGATTCCCCA 
TATGCAGAGA TCAATAACTC AACTTCAGCC AACAGGAATG TCTATGAAGT TGAACCTACA 
GTGAGTGTTG TCCAAGGAGT ATTCAGCAAT AATGGGCGTC TCTCCCAGGA TCCATATGAC 
CTCCCAAAGA ACAGTCACAT CCCTTGTCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA 
TCCTCCCCTA AGCAAGAGGA CAGTGGTGGT AGCAGCAGCA ACAGCAGCAG CAGCAGTGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115822.1
CDS	363..3785
Translation	MVISLNSCLSFICLLLCHWIGTASPLNLEDPNVCSHWESYSVTVQESYPHPFDQIYYTSCTDILNWFKCTRHRVSYRTAYRHGEKTMYRRKSQCCPGFYESGEMCVPHCADKCVHGRCIAPNTCQCEPGWGGTNCSSACDGDHWGPHCTSRCQCKNGALCNPITGACHCAAGFRGWRCEDRCEQGTYGNDCHQRCQCQNGATCDHVTGECRCPPGYTGAFCEDLCPPGKHGPQCEQRCPCQNGGVCHHVTGECSCPSGWMGTVCGQPCPEGRFGKNCSQECQCHNGGTCDAATGQCHCSPGYTGERCQDECPVGTYGVLCAETCQCVNGGKCYHVSGACLCEAGFAGERCEARLCPEGLYGIKCDKRCPCHLENTHSCHPMSGECACKPGWSGLYCNETCSPGFYGEACQQICSCQNGADCDSVTGKCTCAPGFKGIDCSTPCPLGTYGINCSSRCGCKNDAVCSPVDGSCTCKAGWHGVDCSIRCPSGTWGFGCNLTCQCLNGGACNTLDGTCTCAPGWRGEKCELPCQDGTYGLNCAERCDCSHADGCHPTTGHCRCLPGWSGVHCDSVCAEGRWGPNCSLPCYCKNGASCSPDDGICECAPGFRGTTCQRICSPGFYGHRCSQTCPQCVHSSGPCHHITGLCDCLPGFTGALCNEVCPSGRFGKNCAGICTCTNNGTCNPIDRSCQCYPGWIGSDCSQPCPPAHWGPNCIHTCNCHNGAFCSAYDGECKCTPGWTGLYCTQRCPLGFYGKDCALICQCQNGADCDHISGQCTCRTGFMGRHCEQKCPSGTYGYGCRQICDCLNNSTCDHITGTCYCSPGWKGARCDQAGVIIVGNLNSLSRTSTALPADSYQIGAIAGIIILVLVVLFLLALFIIYRHKQKGKESSMPAVTYTPAMRVVNADYTISGTLPHSNGGNANSHYFTNPSYHTLTQCATSPHVNNRDRMTVTKSKNNQLFVNLKNVNPGKRGPVGDCTGTLPADWKHGGYLNELGAFGLDRSYMGKSLKDLGKNSEYNSSNCSLSSSENPYATIKDPPVLIPKSSECGYVEMKSPARRDSPYAEINNSTSANRNVYEVEPTVSVVQGVFSNNGRLSQDPYDLPKNSHIPCHYDLLPVRDSSSSPKQEDSGGSSSNSSSSSE

Target ORF information:

RefSeq Version	NM_032446.2
Organism	Homo sapiens(human)
Definition	Homo sapiens multiple EGF like domains 10 (MEGF10), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032446.2

ORF Insert Sequence:

ATGGTTATTT CTTTGAACTC ATGCCTGAGC TTTATTTGTT TATTGTTATG CCACTGGATT 
GGGACAGCAT CACCTCTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC 
TCAGTGACTG TGCAAGAGTC ATACCCACAT CCCTTTGATC AAATTTACTA CACGAGCTGC 
ACTGACATTC TAAACTGGTT TAAATGCACG CGGCACAGAG TCAGCTATCG GACAGCCTAT 
CGACATGGGG AGAAGACTAT GTATAGGCGC AAGTCTCAGT GTTGTCCTGG ATTTTATGAA 
AGCGGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGTATTGCT 
CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGAGGGACCA ACTGCTCCAG TGCCTGCGAT 
GGTGATCACT GGGGTCCCCA CTGCACCAGC CGGTGCCAGT GCAAAAATGG GGCTCTGTGC 
AACCCCATCA CCGGGGCTTG CCACTGTGCT GCGGGCTTCC GGGGCTGGCG CTGCGAGGAC 
CGCTGTGAGC AGGGCACCTA TGGTAACGAC TGTCATCAGA GATGCCAGTG CCAGAATGGA 
GCCACCTGCG ACCACGTCAC GGGGGAATGC CGCTGCCCAC CAGGATACAC CGGAGCCTTC 
TGTGAGGATC TTTGTCCTCC TGGTAAACAT GGTCCACAGT GTGAGCAGAG ATGCCCTTGT 
CAAAATGGAG GAGTGTGTCA TCACGTCACT GGAGAATGCT CTTGCCCTTC TGGCTGGATG 
GGCACAGTGT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCCCAAGAA 
TGCCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA 
GGATACACAG GGGAACGGTG CCAGGATGAG TGTCCTGTTG GGACCTATGG CGTTCTCTGT 
GCTGAGACCT GCCAGTGTGT CAACGGAGGG AAGTGTTACC ACGTGAGCGG CGCATGCCTC 
TGTGAAGCAG GCTTTGCTGG CGAGCGCTGC GAAGCACGCC TGTGTCCTGA GGGGCTCTAC 
GGCATCAAAT GTGACAAACG GTGTCCCTGC CACCTGGAAA ACACTCATAG CTGTCACCCC 
ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA TGAGACATGT 
TCTCCTGGAT TCTACGGGGA AGCTTGCCAG CAGATCTGCA GCTGCCAAAA TGGGGCAGAC 
TGTGACAGTG TGACTGGAAA GTGCACCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT 
ACCCCATGCC CTCTGGGAAC CTATGGGATA AACTGTTCCT CTCGCTGTGG CTGTAAAAAT 
GATGCAGTCT GCTCTCCTGT GGACGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG 
GACTGCTCCA TCAGATGTCC CAGTGGCACA TGGGGCTTTG GCTGTAACTT AACATGCCAG 
TGCCTCAACG GGGGAGCCTG CAACACCCTG GACGGGACCT GCACGTGTGC ACCTGGATGG 
CGCGGGGAGA AATGCGAACT TCCCTGCCAG GATGGCACGT ACGGGCTGAA CTGTGCTGAG 
CGCTGCGACT GCAGCCACGC AGATGGCTGC CACCCTACCA CGGGCCATTG CCGCTGCCTC 
CCCGGATGGT CAGGTGTCCA CTGTGACAGC GTGTGTGCTG AGGGACGCTG GGGCCCCAAC 
TGCTCCCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCTGATGA TGGCATCTGC 
GAGTGTGCAC CAGGCTTCCG AGGCACCACT TGTCAGAGGA TCTGCTCCCC TGGTTTTTAT 
GGGCATCGCT GCAGCCAGAC ATGCCCACAG TGCGTTCACA GCAGCGGGCC CTGCCACCAC 
ATCACCGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGCG CCCTCTGCAA TGAAGTGTGT 
CCCAGTGGCA GATTTGGGAA AAACTGTGCA GGAATTTGTA CCTGCACCAA CAACGGAACC 
TGTAACCCCA TTGACAGATC TTGTCAGTGT TACCCCGGTT GGATTGGCAG TGACTGCTCT 
CAACCATGTC CACCTGCCCA CTGGGGCCCA AACTGCATCC ACACGTGCAA CTGCCATAAT 
GGAGCTTTCT GCAGCGCCTA CGATGGGGAA TGTAAATGCA CTCCTGGCTG GACAGGGCTC 
TACTGCACTC AGAGATGTCC TCTAGGGTTT TATGGAAAAG ATTGTGCACT GATATGCCAA 
TGTCAAAACG GAGCTGACTG CGACCACATT TCTGGGCAGT GTACTTGCCG CACTGGATTC 
ATGGGACGGC ACTGTGAGCA GAAGTGCCCT TCAGGAACAT ATGGCTATGG CTGTCGCCAG 
ATATGTGATT GTCTGAACAA CTCCACCTGC GACCACATCA CTGGGACCTG TTACTGCAGC 
CCCGGATGGA AGGGAGCGAG ATGTGATCAA GCTGGTGTTA TCATAGTTGG AAATCTGAAC 
AGCTTAAGCC GAACCAGTAC TGCTCTCCCT GCTGATTCCT ACCAGATCGG GGCCATTGCA 
GGCATCATCA TTCTTGTCCT AGTTGTTCTC TTCCTACTGG CATTGTTCAT TATTTATAGA 
CACAAGCAGA AGGGAAAGGA ATCAAGCATG CCAGCAGTTA CCTACACCCC TGCTATGAGG 
GTCGTCAATG CAGATTATAC CATTTCAGGA ACCCTTCCTC ACAGCAATGG TGGAAACGCT 
AATAGCCACT ACTTCACCAA TCCCAGTTAC CACACGCTCA CCCAGTGTGC CACATCCCCT 
CACGTCAACA ACAGGGACAG GATGACTGTC ACGAAGTCAA AAAACAATCA ACTGTTTGTG 
AATCTTAAAA ATGTGAACCC TGGGAAGAGA GGCCCTGTGG GGGACTGCAC TGGGACATTG 
CCGGCTGACT GGAAACATGG CGGCTACCTC AACGAGCTCG GTGCTTTTGG ACTTGACAGA 
AGCTATATGG GAAAATCCTT AAAAGACCTG GGAAAGAATT CTGAATATAA TTCAAGTAAC 
TGCTCCCTAA GCAGTTCTGA GAACCCATAT GCCACTATTA AAGACCCACC TGTACTTATC 
CCGAAAAGCT CAGAGTGTGG TTATGTGGAG ATGAAATCGC CGGCACGAAG AGATTCCCCA 
TATGCAGAGA TCAATAACTC AACTTCAGCC AACAGGAATG TCTATGAAGT TGAACCTACA 
GTGAGTGTTG TCCAAGGAGT ATTCAGCAAT AATGGGCGTC TCTCCCAGGA TCCATATGAC 
CTCCCAAAGA ACAGTCACAT CCCTTGTCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA 
TCCTCCCCTA AGCAAGAGGA CAGTGGTGGT AGCAGCAGCA ACAGCAGCAG CAGCAGTGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEGF10 ( XM_011543694.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541996.1 Homo sapiens multiple EGF like domains 10 (MEGF10), transcript variant X2, mRNA.

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CloneID	OHu12082
Clone ID Related Accession (Same CDS sequence)	NM_001256545.1 , NM_001256545.2 , NM_032446.3 , XM_011543694.1 , NM_032446.2
Accession Version	XM_011543694.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	multiple epidermal growth factor-like domains protein 10 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTATTT CTTTGAACTC ATGCCTGAGC TTTATTTGTT TATTGTTATG CCACTGGATT 
GGGACAGCAT CACCTCTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC 
TCAGTGACTG TGCAAGAGTC ATACCCACAT CCCTTTGATC AAATTTACTA CACGAGCTGC 
ACTGACATTC TAAACTGGTT TAAATGCACG CGGCACAGAG TCAGCTATCG GACAGCCTAT 
CGACATGGGG AGAAGACTAT GTATAGGCGC AAGTCTCAGT GTTGTCCTGG ATTTTATGAA 
AGCGGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGTATTGCT 
CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGAGGGACCA ACTGCTCCAG TGCCTGCGAT 
GGTGATCACT GGGGTCCCCA CTGCACCAGC CGGTGCCAGT GCAAAAATGG GGCTCTGTGC 
AACCCCATCA CCGGGGCTTG CCACTGTGCT GCGGGCTTCC GGGGCTGGCG CTGCGAGGAC 
CGCTGTGAGC AGGGCACCTA TGGTAACGAC TGTCATCAGA GATGCCAGTG CCAGAATGGA 
GCCACCTGCG ACCACGTCAC GGGGGAATGC CGCTGCCCAC CAGGATACAC CGGAGCCTTC 
TGTGAGGATC TTTGTCCTCC TGGTAAACAT GGTCCACAGT GTGAGCAGAG ATGCCCTTGT 
CAAAATGGAG GAGTGTGTCA TCACGTCACT GGAGAATGCT CTTGCCCTTC TGGCTGGATG 
GGCACAGTGT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCCCAAGAA 
TGCCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA 
GGATACACAG GGGAACGGTG CCAGGATGAG TGTCCTGTTG GGACCTATGG CGTTCTCTGT 
GCTGAGACCT GCCAGTGTGT CAACGGAGGG AAGTGTTACC ACGTGAGCGG CGCATGCCTC 
TGTGAAGCAG GCTTTGCTGG CGAGCGCTGC GAAGCACGCC TGTGTCCTGA GGGGCTCTAC 
GGCATCAAAT GTGACAAACG GTGTCCCTGC CACCTGGAAA ACACTCATAG CTGTCACCCC 
ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA TGAGACATGT 
TCTCCTGGAT TCTACGGGGA AGCTTGCCAG CAGATCTGCA GCTGCCAAAA TGGGGCAGAC 
TGTGACAGTG TGACTGGAAA GTGCACCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT 
ACCCCATGCC CTCTGGGAAC CTATGGGATA AACTGTTCCT CTCGCTGTGG CTGTAAAAAT 
GATGCAGTCT GCTCTCCTGT GGACGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG 
GACTGCTCCA TCAGATGTCC CAGTGGCACA TGGGGCTTTG GCTGTAACTT AACATGCCAG 
TGCCTCAACG GGGGAGCCTG CAACACCCTG GACGGGACCT GCACGTGTGC ACCTGGATGG 
CGCGGGGAGA AATGCGAACT TCCCTGCCAG GATGGCACGT ACGGGCTGAA CTGTGCTGAG 
CGCTGCGACT GCAGCCACGC AGATGGCTGC CACCCTACCA CGGGCCATTG CCGCTGCCTC 
CCCGGATGGT CAGGTGTCCA CTGTGACAGC GTGTGTGCTG AGGGACGCTG GGGCCCCAAC 
TGCTCCCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCTGATGA TGGCATCTGC 
GAGTGTGCAC CAGGCTTCCG AGGCACCACT TGTCAGAGGA TCTGCTCCCC TGGTTTTTAT 
GGGCATCGCT GCAGCCAGAC ATGCCCACAG TGCGTTCACA GCAGCGGGCC CTGCCACCAC 
ATCACCGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGCG CCCTCTGCAA TGAAGTGTGT 
CCCAGTGGCA GATTTGGGAA AAACTGTGCA GGAATTTGTA CCTGCACCAA CAACGGAACC 
TGTAACCCCA TTGACAGATC TTGTCAGTGT TACCCCGGTT GGATTGGCAG TGACTGCTCT 
CAACCATGTC CACCTGCCCA CTGGGGCCCA AACTGCATCC ACACGTGCAA CTGCCATAAT 
GGAGCTTTCT GCAGCGCCTA CGATGGGGAA TGTAAATGCA CTCCTGGCTG GACAGGGCTC 
TACTGCACTC AGAGATGTCC TCTAGGGTTT TATGGAAAAG ATTGTGCACT GATATGCCAA 
TGTCAAAACG GAGCTGACTG CGACCACATT TCTGGGCAGT GTACTTGCCG CACTGGATTC 
ATGGGACGGC ACTGTGAGCA GAAGTGCCCT TCAGGAACAT ATGGCTATGG CTGTCGCCAG 
ATATGTGATT GTCTGAACAA CTCCACCTGC GACCACATCA CTGGGACCTG TTACTGCAGC 
CCCGGATGGA AGGGAGCGAG ATGTGATCAA GCTGGTGTTA TCATAGTTGG AAATCTGAAC 
AGCTTAAGCC GAACCAGTAC TGCTCTCCCT GCTGATTCCT ACCAGATCGG GGCCATTGCA 
GGCATCATCA TTCTTGTCCT AGTTGTTCTC TTCCTACTGG CATTGTTCAT TATTTATAGA 
CACAAGCAGA AGGGAAAGGA ATCAAGCATG CCAGCAGTTA CCTACACCCC TGCTATGAGG 
GTCGTCAATG CAGATTATAC CATTTCAGGA ACCCTTCCTC ACAGCAATGG TGGAAACGCT 
AATAGCCACT ACTTCACCAA TCCCAGTTAC CACACGCTCA CCCAGTGTGC CACATCCCCT 
CACGTCAACA ACAGGGACAG GATGACTGTC ACGAAGTCAA AAAACAATCA ACTGTTTGTG 
AATCTTAAAA ATGTGAACCC TGGGAAGAGA GGCCCTGTGG GGGACTGCAC TGGGACATTG 
CCGGCTGACT GGAAACATGG CGGCTACCTC AACGAGCTCG GTGCTTTTGG ACTTGACAGA 
AGCTATATGG GAAAATCCTT AAAAGACCTG GGAAAGAATT CTGAATATAA TTCAAGTAAC 
TGCTCCCTAA GCAGTTCTGA GAACCCATAT GCCACTATTA AAGACCCACC TGTACTTATC 
CCGAAAAGCT CAGAGTGTGG TTATGTGGAG ATGAAATCGC CGGCACGAAG AGATTCCCCA 
TATGCAGAGA TCAATAACTC AACTTCAGCC AACAGGAATG TCTATGAAGT TGAACCTACA 
GTGAGTGTTG TCCAAGGAGT ATTCAGCAAT AATGGGCGTC TCTCCCAGGA TCCATATGAC 
CTCCCAAAGA ACAGTCACAT CCCTTGTCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA 
TCCTCCCCTA AGCAAGAGGA CAGTGGTGGT AGCAGCAGCA ACAGCAGCAG CAGCAGTGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541996.1
CDS	75..3497
Translation	MVISLNSCLSFICLLLCHWIGTASPLNLEDPNVCSHWESYSVTVQESYPHPFDQIYYTSCTDILNWFKCTRHRVSYRTAYRHGEKTMYRRKSQCCPGFYESGEMCVPHCADKCVHGRCIAPNTCQCEPGWGGTNCSSACDGDHWGPHCTSRCQCKNGALCNPITGACHCAAGFRGWRCEDRCEQGTYGNDCHQRCQCQNGATCDHVTGECRCPPGYTGAFCEDLCPPGKHGPQCEQRCPCQNGGVCHHVTGECSCPSGWMGTVCGQPCPEGRFGKNCSQECQCHNGGTCDAATGQCHCSPGYTGERCQDECPVGTYGVLCAETCQCVNGGKCYHVSGACLCEAGFAGERCEARLCPEGLYGIKCDKRCPCHLENTHSCHPMSGECACKPGWSGLYCNETCSPGFYGEACQQICSCQNGADCDSVTGKCTCAPGFKGIDCSTPCPLGTYGINCSSRCGCKNDAVCSPVDGSCTCKAGWHGVDCSIRCPSGTWGFGCNLTCQCLNGGACNTLDGTCTCAPGWRGEKCELPCQDGTYGLNCAERCDCSHADGCHPTTGHCRCLPGWSGVHCDSVCAEGRWGPNCSLPCYCKNGASCSPDDGICECAPGFRGTTCQRICSPGFYGHRCSQTCPQCVHSSGPCHHITGLCDCLPGFTGALCNEVCPSGRFGKNCAGICTCTNNGTCNPIDRSCQCYPGWIGSDCSQPCPPAHWGPNCIHTCNCHNGAFCSAYDGECKCTPGWTGLYCTQRCPLGFYGKDCALICQCQNGADCDHISGQCTCRTGFMGRHCEQKCPSGTYGYGCRQICDCLNNSTCDHITGTCYCSPGWKGARCDQAGVIIVGNLNSLSRTSTALPADSYQIGAIAGIIILVLVVLFLLALFIIYRHKQKGKESSMPAVTYTPAMRVVNADYTISGTLPHSNGGNANSHYFTNPSYHTLTQCATSPHVNNRDRMTVTKSKNNQLFVNLKNVNPGKRGPVGDCTGTLPADWKHGGYLNELGAFGLDRSYMGKSLKDLGKNSEYNSSNCSLSSSENPYATIKDPPVLIPKSSECGYVEMKSPARRDSPYAEINNSTSANRNVYEVEPTVSVVQGVFSNNGRLSQDPYDLPKNSHIPCHYDLLPVRDSSSSPKQEDSGGSSSNSSSSSE

Target ORF information:

RefSeq Version	XM_011543694.1
Organism	Homo sapiens(human)
Definition	Homo sapiens multiple EGF like domains 10 (MEGF10), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543694.1

ORF Insert Sequence:

ATGGTTATTT CTTTGAACTC ATGCCTGAGC TTTATTTGTT TATTGTTATG CCACTGGATT 
GGGACAGCAT CACCTCTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC 
TCAGTGACTG TGCAAGAGTC ATACCCACAT CCCTTTGATC AAATTTACTA CACGAGCTGC 
ACTGACATTC TAAACTGGTT TAAATGCACG CGGCACAGAG TCAGCTATCG GACAGCCTAT 
CGACATGGGG AGAAGACTAT GTATAGGCGC AAGTCTCAGT GTTGTCCTGG ATTTTATGAA 
AGCGGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGTATTGCT 
CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGAGGGACCA ACTGCTCCAG TGCCTGCGAT 
GGTGATCACT GGGGTCCCCA CTGCACCAGC CGGTGCCAGT GCAAAAATGG GGCTCTGTGC 
AACCCCATCA CCGGGGCTTG CCACTGTGCT GCGGGCTTCC GGGGCTGGCG CTGCGAGGAC 
CGCTGTGAGC AGGGCACCTA TGGTAACGAC TGTCATCAGA GATGCCAGTG CCAGAATGGA 
GCCACCTGCG ACCACGTCAC GGGGGAATGC CGCTGCCCAC CAGGATACAC CGGAGCCTTC 
TGTGAGGATC TTTGTCCTCC TGGTAAACAT GGTCCACAGT GTGAGCAGAG ATGCCCTTGT 
CAAAATGGAG GAGTGTGTCA TCACGTCACT GGAGAATGCT CTTGCCCTTC TGGCTGGATG 
GGCACAGTGT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCCCAAGAA 
TGCCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA 
GGATACACAG GGGAACGGTG CCAGGATGAG TGTCCTGTTG GGACCTATGG CGTTCTCTGT 
GCTGAGACCT GCCAGTGTGT CAACGGAGGG AAGTGTTACC ACGTGAGCGG CGCATGCCTC 
TGTGAAGCAG GCTTTGCTGG CGAGCGCTGC GAAGCACGCC TGTGTCCTGA GGGGCTCTAC 
GGCATCAAAT GTGACAAACG GTGTCCCTGC CACCTGGAAA ACACTCATAG CTGTCACCCC 
ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA TGAGACATGT 
TCTCCTGGAT TCTACGGGGA AGCTTGCCAG CAGATCTGCA GCTGCCAAAA TGGGGCAGAC 
TGTGACAGTG TGACTGGAAA GTGCACCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT 
ACCCCATGCC CTCTGGGAAC CTATGGGATA AACTGTTCCT CTCGCTGTGG CTGTAAAAAT 
GATGCAGTCT GCTCTCCTGT GGACGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG 
GACTGCTCCA TCAGATGTCC CAGTGGCACA TGGGGCTTTG GCTGTAACTT AACATGCCAG 
TGCCTCAACG GGGGAGCCTG CAACACCCTG GACGGGACCT GCACGTGTGC ACCTGGATGG 
CGCGGGGAGA AATGCGAACT TCCCTGCCAG GATGGCACGT ACGGGCTGAA CTGTGCTGAG 
CGCTGCGACT GCAGCCACGC AGATGGCTGC CACCCTACCA CGGGCCATTG CCGCTGCCTC 
CCCGGATGGT CAGGTGTCCA CTGTGACAGC GTGTGTGCTG AGGGACGCTG GGGCCCCAAC 
TGCTCCCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCTGATGA TGGCATCTGC 
GAGTGTGCAC CAGGCTTCCG AGGCACCACT TGTCAGAGGA TCTGCTCCCC TGGTTTTTAT 
GGGCATCGCT GCAGCCAGAC ATGCCCACAG TGCGTTCACA GCAGCGGGCC CTGCCACCAC 
ATCACCGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGCG CCCTCTGCAA TGAAGTGTGT 
CCCAGTGGCA GATTTGGGAA AAACTGTGCA GGAATTTGTA CCTGCACCAA CAACGGAACC 
TGTAACCCCA TTGACAGATC TTGTCAGTGT TACCCCGGTT GGATTGGCAG TGACTGCTCT 
CAACCATGTC CACCTGCCCA CTGGGGCCCA AACTGCATCC ACACGTGCAA CTGCCATAAT 
GGAGCTTTCT GCAGCGCCTA CGATGGGGAA TGTAAATGCA CTCCTGGCTG GACAGGGCTC 
TACTGCACTC AGAGATGTCC TCTAGGGTTT TATGGAAAAG ATTGTGCACT GATATGCCAA 
TGTCAAAACG GAGCTGACTG CGACCACATT TCTGGGCAGT GTACTTGCCG CACTGGATTC 
ATGGGACGGC ACTGTGAGCA GAAGTGCCCT TCAGGAACAT ATGGCTATGG CTGTCGCCAG 
ATATGTGATT GTCTGAACAA CTCCACCTGC GACCACATCA CTGGGACCTG TTACTGCAGC 
CCCGGATGGA AGGGAGCGAG ATGTGATCAA GCTGGTGTTA TCATAGTTGG AAATCTGAAC 
AGCTTAAGCC GAACCAGTAC TGCTCTCCCT GCTGATTCCT ACCAGATCGG GGCCATTGCA 
GGCATCATCA TTCTTGTCCT AGTTGTTCTC TTCCTACTGG CATTGTTCAT TATTTATAGA 
CACAAGCAGA AGGGAAAGGA ATCAAGCATG CCAGCAGTTA CCTACACCCC TGCTATGAGG 
GTCGTCAATG CAGATTATAC CATTTCAGGA ACCCTTCCTC ACAGCAATGG TGGAAACGCT 
AATAGCCACT ACTTCACCAA TCCCAGTTAC CACACGCTCA CCCAGTGTGC CACATCCCCT 
CACGTCAACA ACAGGGACAG GATGACTGTC ACGAAGTCAA AAAACAATCA ACTGTTTGTG 
AATCTTAAAA ATGTGAACCC TGGGAAGAGA GGCCCTGTGG GGGACTGCAC TGGGACATTG 
CCGGCTGACT GGAAACATGG CGGCTACCTC AACGAGCTCG GTGCTTTTGG ACTTGACAGA 
AGCTATATGG GAAAATCCTT AAAAGACCTG GGAAAGAATT CTGAATATAA TTCAAGTAAC 
TGCTCCCTAA GCAGTTCTGA GAACCCATAT GCCACTATTA AAGACCCACC TGTACTTATC 
CCGAAAAGCT CAGAGTGTGG TTATGTGGAG ATGAAATCGC CGGCACGAAG AGATTCCCCA 
TATGCAGAGA TCAATAACTC AACTTCAGCC AACAGGAATG TCTATGAAGT TGAACCTACA 
GTGAGTGTTG TCCAAGGAGT ATTCAGCAAT AATGGGCGTC TCTCCCAGGA TCCATATGAC 
CTCCCAAAGA ACAGTCACAT CCCTTGTCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA 
TCCTCCCCTA AGCAAGAGGA CAGTGGTGGT AGCAGCAGCA ACAGCAGCAG CAGCAGTGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEGF10 ( NM_001308119.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001295048.1 Homo sapiens multiple EGF like domains 10 (MEGF10), transcript variant 3, mRNA.

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CloneID	OHu73554
Clone ID Related Accession (Same CDS sequence)	NM_001308119.1 , NM_001308121.1
Accession Version	NM_001308119.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-11
Organism	Homo sapiens(human)
Product	multiple epidermal growth factor-like domains protein 10 isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA240513.1, BC020198.1, AC008682.6 and AK307919.1. On Apr 22, 2015 this sequence version replaced XM_011543695.1. Transcript Variant: This variant (3) lacks several 3' exons but contains an alternate 3' terminal exon, and it thus differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (b) has a distinct C-terminus and is significantly shorter than isoform a. Both variants 3 and 4 encode isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.233143.1, BC020198.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTTATTT CTTTGAACTC ATGCCTGAGC TTTATTTGTT TATTGTTATG CCACTGGATT 
GGGACAGCAT CACCTCTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC 
TCAGTGACTG TGCAAGAGTC ATACCCACAT CCCTTTGATC AAATTTACTA CACGAGCTGC 
ACTGACATTC TAAACTGGTT TAAATGCACG CGGCACAGAG TCAGCTATCG GACAGCCTAT 
CGACATGGGG AGAAGACTAT GTATAGGCGC AAGTCTCAGT GTTGTCCTGG ATTTTATGAA 
AGCGGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGTATTGCT 
CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGAGGGACCA ACTGCTCCAG TGCCTGCGAT 
GGTGATCACT GGGGTCCCCA CTGCACCAGC CGGTGCCAGT GCAAAAATGG GGCTCTGTGC 
AACCCCATCA CCGGGGCTTG CCACTGTGCT GCGGGCTTCC GGGGCTGGCG CTGCGAGGAC 
CGCTGTGAGC AGGGCACCTA TGGTAACGAC TGTCATCAGA GATGCCAGTG CCAGAATGGA 
GCCACCTGCG ACCACGTCAC GGGGGAATGC CGCTGCCCAC CAGGATACAC CGGAGCCTTC 
TGTGAGGATC TTTGTCCTCC TGGTAAACAT GGTCCACAGT GTGAGCAGAG ATGCCCTTGT 
CAAAATGGAG GAGTGTGTCA TCACGTCACT GGAGAATGCT CTTGCCCTTC TGGCTGGATG 
GGCACAGTGT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCCCAAGAA 
TGCCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA 
GGATACACAG GGGAACGGTG CCAGGATGAG TGTCCTGTTG GGACCTATGG CGTTCTCTGT 
GCTGAGACCT GCCAGTGTGT CAACGGAGGG AAGTGTTACC ACGTGAGCGG CGCATGCCTC 
TGTGAAGCAG GCTTTGCTGG CGAGCGCTGC GAAGCACGCC TGTGTCCTGA GGGGCTCTAC 
GGCATCAAAT GTGACAAACG GTGTCCCTGC CACCTGGAAA ACACTCATAG CTGTCACCCC 
ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA TGAGACATGT 
TCTCCTGGAT TCTACGGGGA AGCTTGCCAG CAGATCTGCA GCTGCCAAAA TGGGGCAGAC 
TGTGACAGTG TGACTGGAAA GTGCACCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT 
ACCCCATGCC CTCTGGGAAC CTATGGGATA AACTGTTCCT CTCGCTGTGG CTGTAAAAAT 
GATGCAGTCT GCTCTCCTGT GGACGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG 
GACTGCTCCA TCAGATGTCC CAGTGGCACA TGGGGCTTTG GCTGTAACTT AACATGCCAG 
TGCCTCAACG GGGGAGCCTG CAACACCCTG GACGGGACCT GCACGTGTGC ACCTGGATGG 
CGCGGGGAGA AATGCGAACT TCCCTGCCAG GATGGCACGT ACGGGCTGAA CTGTGCTGAG 
CGCTGCGACT GCAGCCACGC AGATGGCTGC CACCCTACCA CGGGCCATTG CCGCTGCCTC 
CCCGGATGGT CAGGATTGTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001295048.1
CDS	363..2066
Translation	MVISLNSCLSFICLLLCHWIGTASPLNLEDPNVCSHWESYSVTVQESYPHPFDQIYYTSCTDILNWFKCTRHRVSYRTAYRHGEKTMYRRKSQCCPGFYESGEMCVPHCADKCVHGRCIAPNTCQCEPGWGGTNCSSACDGDHWGPHCTSRCQCKNGALCNPITGACHCAAGFRGWRCEDRCEQGTYGNDCHQRCQCQNGATCDHVTGECRCPPGYTGAFCEDLCPPGKHGPQCEQRCPCQNGGVCHHVTGECSCPSGWMGTVCGQPCPEGRFGKNCSQECQCHNGGTCDAATGQCHCSPGYTGERCQDECPVGTYGVLCAETCQCVNGGKCYHVSGACLCEAGFAGERCEARLCPEGLYGIKCDKRCPCHLENTHSCHPMSGECACKPGWSGLYCNETCSPGFYGEACQQICSCQNGADCDSVTGKCTCAPGFKGIDCSTPCPLGTYGINCSSRCGCKNDAVCSPVDGSCTCKAGWHGVDCSIRCPSGTWGFGCNLTCQCLNGGACNTLDGTCTCAPGWRGEKCELPCQDGTYGLNCAERCDCSHADGCHPTTGHCRCLPGWSGLF

Target ORF information:

RefSeq Version	NM_001308119.1
Organism	Homo sapiens(human)
Definition	Homo sapiens multiple EGF like domains 10 (MEGF10), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308119.1

ORF Insert Sequence:

ATGGTTATTT CTTTGAACTC ATGCCTGAGC TTTATTTGTT TATTGTTATG CCACTGGATT 
GGGACAGCAT CACCTCTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC 
TCAGTGACTG TGCAAGAGTC ATACCCACAT CCCTTTGATC AAATTTACTA CACGAGCTGC 
ACTGACATTC TAAACTGGTT TAAATGCACG CGGCACAGAG TCAGCTATCG GACAGCCTAT 
CGACATGGGG AGAAGACTAT GTATAGGCGC AAGTCTCAGT GTTGTCCTGG ATTTTATGAA 
AGCGGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGTATTGCT 
CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGAGGGACCA ACTGCTCCAG TGCCTGCGAT 
GGTGATCACT GGGGTCCCCA CTGCACCAGC CGGTGCCAGT GCAAAAATGG GGCTCTGTGC 
AACCCCATCA CCGGGGCTTG CCACTGTGCT GCGGGCTTCC GGGGCTGGCG CTGCGAGGAC 
CGCTGTGAGC AGGGCACCTA TGGTAACGAC TGTCATCAGA GATGCCAGTG CCAGAATGGA 
GCCACCTGCG ACCACGTCAC GGGGGAATGC CGCTGCCCAC CAGGATACAC CGGAGCCTTC 
TGTGAGGATC TTTGTCCTCC TGGTAAACAT GGTCCACAGT GTGAGCAGAG ATGCCCTTGT 
CAAAATGGAG GAGTGTGTCA TCACGTCACT GGAGAATGCT CTTGCCCTTC TGGCTGGATG 
GGCACAGTGT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCCCAAGAA 
TGCCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA 
GGATACACAG GGGAACGGTG CCAGGATGAG TGTCCTGTTG GGACCTATGG CGTTCTCTGT 
GCTGAGACCT GCCAGTGTGT CAACGGAGGG AAGTGTTACC ACGTGAGCGG CGCATGCCTC 
TGTGAAGCAG GCTTTGCTGG CGAGCGCTGC GAAGCACGCC TGTGTCCTGA GGGGCTCTAC 
GGCATCAAAT GTGACAAACG GTGTCCCTGC CACCTGGAAA ACACTCATAG CTGTCACCCC 
ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA TGAGACATGT 
TCTCCTGGAT TCTACGGGGA AGCTTGCCAG CAGATCTGCA GCTGCCAAAA TGGGGCAGAC 
TGTGACAGTG TGACTGGAAA GTGCACCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT 
ACCCCATGCC CTCTGGGAAC CTATGGGATA AACTGTTCCT CTCGCTGTGG CTGTAAAAAT 
GATGCAGTCT GCTCTCCTGT GGACGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG 
GACTGCTCCA TCAGATGTCC CAGTGGCACA TGGGGCTTTG GCTGTAACTT AACATGCCAG 
TGCCTCAACG GGGGAGCCTG CAACACCCTG GACGGGACCT GCACGTGTGC ACCTGGATGG 
CGCGGGGAGA AATGCGAACT TCCCTGCCAG GATGGCACGT ACGGGCTGAA CTGTGCTGAG 
CGCTGCGACT GCAGCCACGC AGATGGCTGC CACCCTACCA CGGGCCATTG CCGCTGCCTC 
CCCGGATGGT CAGGATTGTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEGF10 ( NM_001308121.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001295050.1 Homo sapiens multiple EGF like domains 10 (MEGF10), transcript variant 4, mRNA.

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CloneID	OHu73554
Clone ID Related Accession (Same CDS sequence)	NM_001308119.1 , NM_001308121.1
Accession Version	NM_001308121.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1704bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-11
Organism	Homo sapiens(human)
Product	multiple epidermal growth factor-like domains protein 10 isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA240513.1, AK307919.1, AC008682.6 and BC020198.1. Transcript Variant: This variant (4) differs in the 5' UTR, and lacks several 3' exons but contains an alternate 3' terminal exon, and it thus differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (b) has a distinct C-terminus and is significantly shorter than isoform a. Both variants 3 and 4 encode isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK307919.1, SRR1660805.137518.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTTATTT CTTTGAACTC ATGCCTGAGC TTTATTTGTT TATTGTTATG CCACTGGATT 
GGGACAGCAT CACCTCTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC 
TCAGTGACTG TGCAAGAGTC ATACCCACAT CCCTTTGATC AAATTTACTA CACGAGCTGC 
ACTGACATTC TAAACTGGTT TAAATGCACG CGGCACAGAG TCAGCTATCG GACAGCCTAT 
CGACATGGGG AGAAGACTAT GTATAGGCGC AAGTCTCAGT GTTGTCCTGG ATTTTATGAA 
AGCGGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGTATTGCT 
CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGAGGGACCA ACTGCTCCAG TGCCTGCGAT 
GGTGATCACT GGGGTCCCCA CTGCACCAGC CGGTGCCAGT GCAAAAATGG GGCTCTGTGC 
AACCCCATCA CCGGGGCTTG CCACTGTGCT GCGGGCTTCC GGGGCTGGCG CTGCGAGGAC 
CGCTGTGAGC AGGGCACCTA TGGTAACGAC TGTCATCAGA GATGCCAGTG CCAGAATGGA 
GCCACCTGCG ACCACGTCAC GGGGGAATGC CGCTGCCCAC CAGGATACAC CGGAGCCTTC 
TGTGAGGATC TTTGTCCTCC TGGTAAACAT GGTCCACAGT GTGAGCAGAG ATGCCCTTGT 
CAAAATGGAG GAGTGTGTCA TCACGTCACT GGAGAATGCT CTTGCCCTTC TGGCTGGATG 
GGCACAGTGT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCCCAAGAA 
TGCCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA 
GGATACACAG GGGAACGGTG CCAGGATGAG TGTCCTGTTG GGACCTATGG CGTTCTCTGT 
GCTGAGACCT GCCAGTGTGT CAACGGAGGG AAGTGTTACC ACGTGAGCGG CGCATGCCTC 
TGTGAAGCAG GCTTTGCTGG CGAGCGCTGC GAAGCACGCC TGTGTCCTGA GGGGCTCTAC 
GGCATCAAAT GTGACAAACG GTGTCCCTGC CACCTGGAAA ACACTCATAG CTGTCACCCC 
ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA TGAGACATGT 
TCTCCTGGAT TCTACGGGGA AGCTTGCCAG CAGATCTGCA GCTGCCAAAA TGGGGCAGAC 
TGTGACAGTG TGACTGGAAA GTGCACCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT 
ACCCCATGCC CTCTGGGAAC CTATGGGATA AACTGTTCCT CTCGCTGTGG CTGTAAAAAT 
GATGCAGTCT GCTCTCCTGT GGACGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG 
GACTGCTCCA TCAGATGTCC CAGTGGCACA TGGGGCTTTG GCTGTAACTT AACATGCCAG 
TGCCTCAACG GGGGAGCCTG CAACACCCTG GACGGGACCT GCACGTGTGC ACCTGGATGG 
CGCGGGGAGA AATGCGAACT TCCCTGCCAG GATGGCACGT ACGGGCTGAA CTGTGCTGAG 
CGCTGCGACT GCAGCCACGC AGATGGCTGC CACCCTACCA CGGGCCATTG CCGCTGCCTC 
CCCGGATGGT CAGGATTGTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001295050.1
CDS	312..2015
Translation	MVISLNSCLSFICLLLCHWIGTASPLNLEDPNVCSHWESYSVTVQESYPHPFDQIYYTSCTDILNWFKCTRHRVSYRTAYRHGEKTMYRRKSQCCPGFYESGEMCVPHCADKCVHGRCIAPNTCQCEPGWGGTNCSSACDGDHWGPHCTSRCQCKNGALCNPITGACHCAAGFRGWRCEDRCEQGTYGNDCHQRCQCQNGATCDHVTGECRCPPGYTGAFCEDLCPPGKHGPQCEQRCPCQNGGVCHHVTGECSCPSGWMGTVCGQPCPEGRFGKNCSQECQCHNGGTCDAATGQCHCSPGYTGERCQDECPVGTYGVLCAETCQCVNGGKCYHVSGACLCEAGFAGERCEARLCPEGLYGIKCDKRCPCHLENTHSCHPMSGECACKPGWSGLYCNETCSPGFYGEACQQICSCQNGADCDSVTGKCTCAPGFKGIDCSTPCPLGTYGINCSSRCGCKNDAVCSPVDGSCTCKAGWHGVDCSIRCPSGTWGFGCNLTCQCLNGGACNTLDGTCTCAPGWRGEKCELPCQDGTYGLNCAERCDCSHADGCHPTTGHCRCLPGWSGLF

Target ORF information:

RefSeq Version	NM_001308121.1
Organism	Homo sapiens(human)
Definition	Homo sapiens multiple EGF like domains 10 (MEGF10), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001308121.1

ORF Insert Sequence:

ATGGTTATTT CTTTGAACTC ATGCCTGAGC TTTATTTGTT TATTGTTATG CCACTGGATT 
GGGACAGCAT CACCTCTGAA TCTTGAAGAC CCTAATGTGT GTAGCCACTG GGAAAGCTAC 
TCAGTGACTG TGCAAGAGTC ATACCCACAT CCCTTTGATC AAATTTACTA CACGAGCTGC 
ACTGACATTC TAAACTGGTT TAAATGCACG CGGCACAGAG TCAGCTATCG GACAGCCTAT 
CGACATGGGG AGAAGACTAT GTATAGGCGC AAGTCTCAGT GTTGTCCTGG ATTTTATGAA 
AGCGGGGAAA TGTGTGTCCC CCACTGTGCT GATAAATGTG TCCATGGTCG CTGTATTGCT 
CCAAACACCT GTCAGTGTGA GCCTGGCTGG GGAGGGACCA ACTGCTCCAG TGCCTGCGAT 
GGTGATCACT GGGGTCCCCA CTGCACCAGC CGGTGCCAGT GCAAAAATGG GGCTCTGTGC 
AACCCCATCA CCGGGGCTTG CCACTGTGCT GCGGGCTTCC GGGGCTGGCG CTGCGAGGAC 
CGCTGTGAGC AGGGCACCTA TGGTAACGAC TGTCATCAGA GATGCCAGTG CCAGAATGGA 
GCCACCTGCG ACCACGTCAC GGGGGAATGC CGCTGCCCAC CAGGATACAC CGGAGCCTTC 
TGTGAGGATC TTTGTCCTCC TGGTAAACAT GGTCCACAGT GTGAGCAGAG ATGCCCTTGT 
CAAAATGGAG GAGTGTGTCA TCACGTCACT GGAGAATGCT CTTGCCCTTC TGGCTGGATG 
GGCACAGTGT GTGGTCAGCC TTGCCCCGAG GGTCGCTTTG GAAAGAACTG TTCCCAAGAA 
TGCCAGTGCC ATAATGGAGG GACGTGTGAT GCTGCCACAG GCCAATGTCA TTGCAGTCCA 
GGATACACAG GGGAACGGTG CCAGGATGAG TGTCCTGTTG GGACCTATGG CGTTCTCTGT 
GCTGAGACCT GCCAGTGTGT CAACGGAGGG AAGTGTTACC ACGTGAGCGG CGCATGCCTC 
TGTGAAGCAG GCTTTGCTGG CGAGCGCTGC GAAGCACGCC TGTGTCCTGA GGGGCTCTAC 
GGCATCAAAT GTGACAAACG GTGTCCCTGC CACCTGGAAA ACACTCATAG CTGTCACCCC 
ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA TGAGACATGT 
TCTCCTGGAT TCTACGGGGA AGCTTGCCAG CAGATCTGCA GCTGCCAAAA TGGGGCAGAC 
TGTGACAGTG TGACTGGAAA GTGCACCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT 
ACCCCATGCC CTCTGGGAAC CTATGGGATA AACTGTTCCT CTCGCTGTGG CTGTAAAAAT 
GATGCAGTCT GCTCTCCTGT GGACGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG 
GACTGCTCCA TCAGATGTCC CAGTGGCACA TGGGGCTTTG GCTGTAACTT AACATGCCAG 
TGCCTCAACG GGGGAGCCTG CAACACCCTG GACGGGACCT GCACGTGTGC ACCTGGATGG 
CGCGGGGAGA AATGCGAACT TCCCTGCCAG GATGGCACGT ACGGGCTGAA CTGTGCTGAG 
CGCTGCGACT GCAGCCACGC AGATGGCTGC CACCCTACCA CGGGCCATTG CCGCTGCCTC 
CCCGGATGGT CAGGATTGTT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEGF10 ( XM_017009988.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865477.1 Homo sapiens multiple EGF like domains 10 (MEGF10), transcript variant X3, mRNA.

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CloneID	OHu96164
Clone ID Related Accession (Same CDS sequence)	XM_017009988.1
Accession Version	XM_017009988.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2283bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	multiple epidermal growth factor-like domains protein 10 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA TGAGACATGT 
TCTCCTGGAT TCTACGGGGA AGCTTGCCAG CAGATCTGCA GCTGCCAAAA TGGGGCAGAC 
TGTGACAGTG TGACTGGAAA GTGCACCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT 
ACCCCATGCC CTCTGGGAAC CTATGGGATA AACTGTTCCT CTCGCTGTGG CTGTAAAAAT 
GATGCAGTCT GCTCTCCTGT GGACGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG 
GACTGCTCCA TCAGATGTCC CAGTGGCACA TGGGGCTTTG GCTGTAACTT AACATGCCAG 
TGCCTCAACG GGGGAGCCTG CAACACCCTG GACGGGACCT GCACGTGTGC ACCTGGATGG 
CGCGGGGAGA AATGCGAACT TCCCTGCCAG GATGGCACGT ACGGGCTGAA CTGTGCTGAG 
CGCTGCGACT GCAGCCACGC AGATGGCTGC CACCCTACCA CGGGCCATTG CCGCTGCCTC 
CCCGGATGGT CAGGTGTCCA CTGTGACAGC GTGTGTGCTG AGGGACGCTG GGGCCCCAAC 
TGCTCCCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCTGATGA TGGCATCTGC 
GAGTGTGCAC CAGGCTTCCG AGGCACCACT TGTCAGAGGA TCTGCTCCCC TGGTTTTTAT 
GGGCATCGCT GCAGCCAGAC ATGCCCACAG TGCGTTCACA GCAGCGGGCC CTGCCACCAC 
ATCACCGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGCG CCCTCTGCAA TGAAGTGTGT 
CCCAGTGGCA GATTTGGGAA AAACTGTGCA GGAATTTGTA CCTGCACCAA CAACGGAACC 
TGTAACCCCA TTGACAGATC TTGTCAGTGT TACCCCGGTT GGATTGGCAG TGACTGCTCT 
CAACCATGTC CACCTGCCCA CTGGGGCCCA AACTGCATCC ACACGTGCAA CTGCCATAAT 
GGAGCTTTCT GCAGCGCCTA CGATGGGGAA TGTAAATGCA CTCCTGGCTG GACAGGGCTC 
TACTGCACTC AGAGATGTCC TCTAGGGTTT TATGGAAAAG ATTGTGCACT GATATGCCAA 
TGTCAAAACG GAGCTGACTG CGACCACATT TCTGGGCAGT GTACTTGCCG CACTGGATTC 
ATGGGACGGC ACTGTGAGCA GAAGTGCCCT TCAGGAACAT ATGGCTATGG CTGTCGCCAG 
ATATGTGATT GTCTGAACAA CTCCACCTGC GACCACATCA CTGGGACCTG TTACTGCAGC 
CCCGGATGGA AGGGAGCGAG ATGTGATCAA GCTGGTGTTA TCATAGTTGG AAATCTGAAC 
AGCTTAAGCC GAACCAGTAC TGCTCTCCCT GCTGATTCCT ACCAGATCGG GGCCATTGCA 
GGCATCATCA TTCTTGTCCT AGTTGTTCTC TTCCTACTGG CATTGTTCAT TATTTATAGA 
CACAAGCAGA AGGGAAAGGA ATCAAGCATG CCAGCAGTTA CCTACACCCC TGCTATGAGG 
GTCGTCAATG CAGATTATAC CATTTCAGGA ACCCTTCCTC ACAGCAATGG TGGAAACGCT 
AATAGCCACT ACTTCACCAA TCCCAGTTAC CACACGCTCA CCCAGTGTGC CACATCCCCT 
CACGTCAACA ACAGGGACAG GATGACTGTC ACGAAGTCAA AAAACAATCA ACTGTTTGTG 
AATCTTAAAA ATGTGAACCC TGGGAAGAGA GGCCCTGTGG GGGACTGCAC TGGGACATTG 
CCGGCTGACT GGAAACATGG CGGCTACCTC AACGAGCTCG GTGCTTTTGG ACTTGACAGA 
AGCTATATGG GAAAATCCTT AAAAGACCTG GGAAAGAATT CTGAATATAA TTCAAGTAAC 
TGCTCCCTAA GCAGTTCTGA GAACCCATAT GCCACTATTA AAGACCCACC TGTACTTATC 
CCGAAAAGCT CAGAGTGTGG TTATGTGGAG ATGAAATCGC CGGCACGAAG AGATTCCCCA 
TATGCAGAGA TCAATAACTC AACTTCAGCC AACAGGAATG TCTATGAAGT TGAACCTACA 
GTGAGTGTTG TCCAAGGAGT ATTCAGCAAT AATGGGCGTC TCTCCCAGGA TCCATATGAC 
CTCCCAAAGA ACAGTCACAT CCCTTGTCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA 
TCCTCCCCTA AGCAAGAGGA CAGTGGTGGT AGCAGCAGCA ACAGCAGCAG CAGCAGTGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865477.1
CDS	521..2803
Translation	MSGECACKPGWSGLYCNETCSPGFYGEACQQICSCQNGADCDSVTGKCTCAPGFKGIDCSTPCPLGTYGINCSSRCGCKNDAVCSPVDGSCTCKAGWHGVDCSIRCPSGTWGFGCNLTCQCLNGGACNTLDGTCTCAPGWRGEKCELPCQDGTYGLNCAERCDCSHADGCHPTTGHCRCLPGWSGVHCDSVCAEGRWGPNCSLPCYCKNGASCSPDDGICECAPGFRGTTCQRICSPGFYGHRCSQTCPQCVHSSGPCHHITGLCDCLPGFTGALCNEVCPSGRFGKNCAGICTCTNNGTCNPIDRSCQCYPGWIGSDCSQPCPPAHWGPNCIHTCNCHNGAFCSAYDGECKCTPGWTGLYCTQRCPLGFYGKDCALICQCQNGADCDHISGQCTCRTGFMGRHCEQKCPSGTYGYGCRQICDCLNNSTCDHITGTCYCSPGWKGARCDQAGVIIVGNLNSLSRTSTALPADSYQIGAIAGIIILVLVVLFLLALFIIYRHKQKGKESSMPAVTYTPAMRVVNADYTISGTLPHSNGGNANSHYFTNPSYHTLTQCATSPHVNNRDRMTVTKSKNNQLFVNLKNVNPGKRGPVGDCTGTLPADWKHGGYLNELGAFGLDRSYMGKSLKDLGKNSEYNSSNCSLSSSENPYATIKDPPVLIPKSSECGYVEMKSPARRDSPYAEINNSTSANRNVYEVEPTVSVVQGVFSNNGRLSQDPYDLPKNSHIPCHYDLLPVRDSSSSPKQEDSGGSSSNSSSSSE

Target ORF information:

RefSeq Version	XM_017009988.1
Organism	Homo sapiens(human)
Definition	Homo sapiens multiple EGF like domains 10 (MEGF10), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009988.1

ORF Insert Sequence:

ATGTCTGGAG AGTGTGCCTG CAAGCCGGGC TGGTCAGGAC TCTACTGTAA TGAGACATGT 
TCTCCTGGAT TCTACGGGGA AGCTTGCCAG CAGATCTGCA GCTGCCAAAA TGGGGCAGAC 
TGTGACAGTG TGACTGGAAA GTGCACCTGT GCCCCAGGAT TCAAAGGAAT TGACTGCTCT 
ACCCCATGCC CTCTGGGAAC CTATGGGATA AACTGTTCCT CTCGCTGTGG CTGTAAAAAT 
GATGCAGTCT GCTCTCCTGT GGACGGGTCT TGTACTTGCA AGGCAGGCTG GCACGGGGTG 
GACTGCTCCA TCAGATGTCC CAGTGGCACA TGGGGCTTTG GCTGTAACTT AACATGCCAG 
TGCCTCAACG GGGGAGCCTG CAACACCCTG GACGGGACCT GCACGTGTGC ACCTGGATGG 
CGCGGGGAGA AATGCGAACT TCCCTGCCAG GATGGCACGT ACGGGCTGAA CTGTGCTGAG 
CGCTGCGACT GCAGCCACGC AGATGGCTGC CACCCTACCA CGGGCCATTG CCGCTGCCTC 
CCCGGATGGT CAGGTGTCCA CTGTGACAGC GTGTGTGCTG AGGGACGCTG GGGCCCCAAC 
TGCTCCCTGC CCTGCTACTG TAAAAATGGG GCTTCATGCT CCCCTGATGA TGGCATCTGC 
GAGTGTGCAC CAGGCTTCCG AGGCACCACT TGTCAGAGGA TCTGCTCCCC TGGTTTTTAT 
GGGCATCGCT GCAGCCAGAC ATGCCCACAG TGCGTTCACA GCAGCGGGCC CTGCCACCAC 
ATCACCGGCC TGTGTGACTG CTTGCCTGGC TTCACAGGCG CCCTCTGCAA TGAAGTGTGT 
CCCAGTGGCA GATTTGGGAA AAACTGTGCA GGAATTTGTA CCTGCACCAA CAACGGAACC 
TGTAACCCCA TTGACAGATC TTGTCAGTGT TACCCCGGTT GGATTGGCAG TGACTGCTCT 
CAACCATGTC CACCTGCCCA CTGGGGCCCA AACTGCATCC ACACGTGCAA CTGCCATAAT 
GGAGCTTTCT GCAGCGCCTA CGATGGGGAA TGTAAATGCA CTCCTGGCTG GACAGGGCTC 
TACTGCACTC AGAGATGTCC TCTAGGGTTT TATGGAAAAG ATTGTGCACT GATATGCCAA 
TGTCAAAACG GAGCTGACTG CGACCACATT TCTGGGCAGT GTACTTGCCG CACTGGATTC 
ATGGGACGGC ACTGTGAGCA GAAGTGCCCT TCAGGAACAT ATGGCTATGG CTGTCGCCAG 
ATATGTGATT GTCTGAACAA CTCCACCTGC GACCACATCA CTGGGACCTG TTACTGCAGC 
CCCGGATGGA AGGGAGCGAG ATGTGATCAA GCTGGTGTTA TCATAGTTGG AAATCTGAAC 
AGCTTAAGCC GAACCAGTAC TGCTCTCCCT GCTGATTCCT ACCAGATCGG GGCCATTGCA 
GGCATCATCA TTCTTGTCCT AGTTGTTCTC TTCCTACTGG CATTGTTCAT TATTTATAGA 
CACAAGCAGA AGGGAAAGGA ATCAAGCATG CCAGCAGTTA CCTACACCCC TGCTATGAGG 
GTCGTCAATG CAGATTATAC CATTTCAGGA ACCCTTCCTC ACAGCAATGG TGGAAACGCT 
AATAGCCACT ACTTCACCAA TCCCAGTTAC CACACGCTCA CCCAGTGTGC CACATCCCCT 
CACGTCAACA ACAGGGACAG GATGACTGTC ACGAAGTCAA AAAACAATCA ACTGTTTGTG 
AATCTTAAAA ATGTGAACCC TGGGAAGAGA GGCCCTGTGG GGGACTGCAC TGGGACATTG 
CCGGCTGACT GGAAACATGG CGGCTACCTC AACGAGCTCG GTGCTTTTGG ACTTGACAGA 
AGCTATATGG GAAAATCCTT AAAAGACCTG GGAAAGAATT CTGAATATAA TTCAAGTAAC 
TGCTCCCTAA GCAGTTCTGA GAACCCATAT GCCACTATTA AAGACCCACC TGTACTTATC 
CCGAAAAGCT CAGAGTGTGG TTATGTGGAG ATGAAATCGC CGGCACGAAG AGATTCCCCA 
TATGCAGAGA TCAATAACTC AACTTCAGCC AACAGGAATG TCTATGAAGT TGAACCTACA 
GTGAGTGTTG TCCAAGGAGT ATTCAGCAAT AATGGGCGTC TCTCCCAGGA TCCATATGAC 
CTCCCAAAGA ACAGTCACAT CCCTTGTCAT TATGACCTGC TGCCAGTCCG AGACAGTTCA 
TCCTCCCCTA AGCAAGAGGA CAGTGGTGGT AGCAGCAGCA ACAGCAGCAG CAGCAGTGAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEGF10 ( XM_017009987.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865476.1 Homo sapiens multiple EGF like domains 10 (MEGF10), transcript variant X1, mRNA.

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CloneID	OHu96163
Clone ID Related Accession (Same CDS sequence)	XM_017009987.1
Accession Version	XM_017009987.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	multiple epidermal growth factor-like domains protein 10 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCAA AGGAAGTAAG CCCGGGAAGA GAAAGCAGTT TTTGGACAAC AGTTCCTTCA 
CCTCAGCATT TTTCATGGAT TTCAAATAAG TGTGCCACCA TGGAGTTTGT GGAGAGTTGG 
ACTGAACCAG CACTGGGATT TTGCCGGGTT GTTCTTCAGA AAAAAATGGT TATTTCTTTG 
AACTCATGCC TGAGCTTTAT TTGTTTATTG TTATGCCACT GGATTGGGAC AGCATCACCT 
CTGAATCTTG AAGACCCTAA TGTGTGTAGC CACTGGGAAA GCTACTCAGT GACTGTGCAA 
GAGTCATACC CACATCCCTT TGATCAAATT TACTACACGA GCTGCACTGA CATTCTAAAC 
TGGTTTAAAT GCACGCGGCA CAGAGTCAGC TATCGGACAG CCTATCGACA TGGGGAGAAG 
ACTATGTATA GGCGCAAGTC TCAGTGTTGT CCTGGATTTT ATGAAAGCGG GGAAATGTGT 
GTCCCCCACT GTGCTGATAA ATGTGTCCAT GGTCGCTGTA TTGCTCCAAA CACCTGTCAG 
TGTGAGCCTG GCTGGGGAGG GACCAACTGC TCCAGTGCCT GCGATGGTGA TCACTGGGGT 
CCCCACTGCA CCAGCCGGTG CCAGTGCAAA AATGGGGCTC TGTGCAACCC CATCACCGGG 
GCTTGCCACT GTGCTGCGGG CTTCCGGGGC TGGCGCTGCG AGGACCGCTG TGAGCAGGGC 
ACCTATGGTA ACGACTGTCA TCAGAGATGC CAGTGCCAGA ATGGAGCCAC CTGCGACCAC 
GTCACGGGGG AATGCCGCTG CCCACCAGGA TACACCGGAG CCTTCTGTGA GGATCTTTGT 
CCTCCTGGTA AACATGGTCC ACAGTGTGAG CAGAGATGCC CTTGTCAAAA TGGAGGAGTG 
TGTCATCACG TCACTGGAGA ATGCTCTTGC CCTTCTGGCT GGATGGGCAC AGTGTGTGGT 
CAGCCTTGCC CCGAGGGTCG CTTTGGAAAG AACTGTTCCC AAGAATGCCA GTGCCATAAT 
GGAGGGACGT GTGATGCTGC CACAGGCCAA TGTCATTGCA GTCCAGGATA CACAGGGGAA 
CGGTGCCAGG ATGAGTGTCC TGTTGGGACC TATGGCGTTC TCTGTGCTGA GACCTGCCAG 
TGTGTCAACG GAGGGAAGTG TTACCACGTG AGCGGCGCAT GCCTCTGTGA AGCAGGCTTT 
GCTGGCGAGC GCTGCGAAGC ACGCCTGTGT CCTGAGGGGC TCTACGGCAT CAAATGTGAC 
AAACGGTGTC CCTGCCACCT GGAAAACACT CATAGCTGTC ACCCCATGTC TGGAGAGTGT 
GCCTGCAAGC CGGGCTGGTC AGGACTCTAC TGTAATGAGA CATGTTCTCC TGGATTCTAC 
GGGGAAGCTT GCCAGCAGAT CTGCAGCTGC CAAAATGGGG CAGACTGTGA CAGTGTGACT 
GGAAAGTGCA CCTGTGCCCC AGGATTCAAA GGAATTGACT GCTCTACCCC ATGCCCTCTG 
GGAACCTATG GGATAAACTG TTCCTCTCGC TGTGGCTGTA AAAATGATGC AGTCTGCTCT 
CCTGTGGACG GGTCTTGTAC TTGCAAGGCA GGCTGGCACG GGGTGGACTG CTCCATCAGA 
TGTCCCAGTG GCACATGGGG CTTTGGCTGT AACTTAACAT GCCAGTGCCT CAACGGGGGA 
GCCTGCAACA CCCTGGACGG GACCTGCACG TGTGCACCTG GATGGCGCGG GGAGAAATGC 
GAACTTCCCT GCCAGGATGG CACGTACGGG CTGAACTGTG CTGAGCGCTG CGACTGCAGC 
CACGCAGATG GCTGCCACCC TACCACGGGC CATTGCCGCT GCCTCCCCGG ATGGTCAGGT 
GTCCACTGTG ACAGCGTGTG TGCTGAGGGA CGCTGGGGCC CCAACTGCTC CCTGCCCTGC 
TACTGTAAAA ATGGGGCTTC ATGCTCCCCT GATGATGGCA TCTGCGAGTG TGCACCAGGC 
TTCCGAGGCA CCACTTGTCA GAGGATCTGC TCCCCTGGTT TTTATGGGCA TCGCTGCAGC 
CAGACATGCC CACAGTGCGT TCACAGCAGC GGGCCCTGCC ACCACATCAC CGGCCTGTGT 
GACTGCTTGC CTGGCTTCAC AGGCGCCCTC TGCAATGAAG TGTGTCCCAG TGGCAGATTT 
GGGAAAAACT GTGCAGGAAT TTGTACCTGC ACCAACAACG GAACCTGTAA CCCCATTGAC 
AGATCTTGTC AGTGTTACCC CGGTTGGATT GGCAGTGACT GCTCTCAACC ATGTCCACCT 
GCCCACTGGG GCCCAAACTG CATCCACACG TGCAACTGCC ATAATGGAGC TTTCTGCAGC 
GCCTACGATG GGGAATGTAA ATGCACTCCT GGCTGGACAG GGCTCTACTG CACTCAGAGA 
TGTCCTCTAG GGTTTTATGG AAAAGATTGT GCACTGATAT GCCAATGTCA AAACGGAGCT 
GACTGCGACC ACATTTCTGG GCAGTGTACT TGCCGCACTG GATTCATGGG ACGGCACTGT 
GAGCAGAAGT GCCCTTCAGG AACATATGGC TATGGCTGTC GCCAGATATG TGATTGTCTG 
AACAACTCCA CCTGCGACCA CATCACTGGG ACCTGTTACT GCAGCCCCGG ATGGAAGGGA 
GCGAGATGTG ATCAAGCTGG TGTTATCATA GTTGGAAATC TGAACAGCTT AAGCCGAACC 
AGTACTGCTC TCCCTGCTGA TTCCTACCAG ATCGGGGCCA TTGCAGGCAT CATCATTCTT 
GTCCTAGTTG TTCTCTTCCT ACTGGCATTG TTCATTATTT ATAGACACAA GCAGAAGGGA 
AAGGAATCAA GCATGCCAGC AGTTACCTAC ACCCCTGCTA TGAGGGTCGT CAATGCAGAT 
TATACCATTT CAGGAACCCT TCCTCACAGC AATGGTGGAA ACGCTAATAG CCACTACTTC 
ACCAATCCCA GTTACCACAC GCTCACCCAG TGTGCCACAT CCCCTCACGT CAACAACAGG 
GACAGGATGA CTGTCACGAA GTCAAAAAAC AATCAACTGT TTGTGAATCT TAAAAATGTG 
AACCCTGGGA AGAGAGGCCC TGTGGGGGAC TGCACTGGGA CATTGCCGGC TGACTGGAAA 
CATGGCGGCT ACCTCAACGA GCTCGGTGCT TTTGGACTTG ACAGAAGCTA TATGGGAAAA 
TCCTTAAAAG ACCTGGGAAA GAATTCTGAA TATAATTCAA GTAACTGCTC CCTAAGCAGT 
TCTGAGAACC CATATGCCAC TATTAAAGAC CCACCTGTAC TTATCCCGAA AAGCTCAGAG 
TGTGGTTATG TGGAGATGAA ATCGCCGGCA CGAAGAGATT CCCCATATGC AGAGATCAAT 
AACTCAACTT CAGCCAACAG GAATGTCTAT GAAGTTGAAC CTACAGTGAG TGTTGTCCAA 
GGAGTATTCA GCAATAATGG GCGTCTCTCC CAGGATCCAT ATGACCTCCC AAAGAACAGT 
CACATCCCTT GTCATTATGA CCTGCTGCCA GTCCGAGACA GTTCATCCTC CCCTAAGCAA 
GAGGACAGTG GTGGTAGCAG CAGCAACAGC AGCAGCAGCA GTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865476.1
CDS	443..4030
Translation	MKAKEVSPGRESSFWTTVPSPQHFSWISNKCATMEFVESWTEPALGFCRVVLQKKMVISLNSCLSFICLLLCHWIGTASPLNLEDPNVCSHWESYSVTVQESYPHPFDQIYYTSCTDILNWFKCTRHRVSYRTAYRHGEKTMYRRKSQCCPGFYESGEMCVPHCADKCVHGRCIAPNTCQCEPGWGGTNCSSACDGDHWGPHCTSRCQCKNGALCNPITGACHCAAGFRGWRCEDRCEQGTYGNDCHQRCQCQNGATCDHVTGECRCPPGYTGAFCEDLCPPGKHGPQCEQRCPCQNGGVCHHVTGECSCPSGWMGTVCGQPCPEGRFGKNCSQECQCHNGGTCDAATGQCHCSPGYTGERCQDECPVGTYGVLCAETCQCVNGGKCYHVSGACLCEAGFAGERCEARLCPEGLYGIKCDKRCPCHLENTHSCHPMSGECACKPGWSGLYCNETCSPGFYGEACQQICSCQNGADCDSVTGKCTCAPGFKGIDCSTPCPLGTYGINCSSRCGCKNDAVCSPVDGSCTCKAGWHGVDCSIRCPSGTWGFGCNLTCQCLNGGACNTLDGTCTCAPGWRGEKCELPCQDGTYGLNCAERCDCSHADGCHPTTGHCRCLPGWSGVHCDSVCAEGRWGPNCSLPCYCKNGASCSPDDGICECAPGFRGTTCQRICSPGFYGHRCSQTCPQCVHSSGPCHHITGLCDCLPGFTGALCNEVCPSGRFGKNCAGICTCTNNGTCNPIDRSCQCYPGWIGSDCSQPCPPAHWGPNCIHTCNCHNGAFCSAYDGECKCTPGWTGLYCTQRCPLGFYGKDCALICQCQNGADCDHISGQCTCRTGFMGRHCEQKCPSGTYGYGCRQICDCLNNSTCDHITGTCYCSPGWKGARCDQAGVIIVGNLNSLSRTSTALPADSYQIGAIAGIIILVLVVLFLLALFIIYRHKQKGKESSMPAVTYTPAMRVVNADYTISGTLPHSNGGNANSHYFTNPSYHTLTQCATSPHVNNRDRMTVTKSKNNQLFVNLKNVNPGKRGPVGDCTGTLPADWKHGGYLNELGAFGLDRSYMGKSLKDLGKNSEYNSSNCSLSSSENPYATIKDPPVLIPKSSECGYVEMKSPARRDSPYAEINNSTSANRNVYEVEPTVSVVQGVFSNNGRLSQDPYDLPKNSHIPCHYDLLPVRDSSSSPKQEDSGGSSSNSSSSSE

Target ORF information:

RefSeq Version	XM_017009987.1
Organism	Homo sapiens(human)
Definition	Homo sapiens multiple EGF like domains 10 (MEGF10), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009987.1

ORF Insert Sequence:

ATGAAGGCAA AGGAAGTAAG CCCGGGAAGA GAAAGCAGTT TTTGGACAAC AGTTCCTTCA 
CCTCAGCATT TTTCATGGAT TTCAAATAAG TGTGCCACCA TGGAGTTTGT GGAGAGTTGG 
ACTGAACCAG CACTGGGATT TTGCCGGGTT GTTCTTCAGA AAAAAATGGT TATTTCTTTG 
AACTCATGCC TGAGCTTTAT TTGTTTATTG TTATGCCACT GGATTGGGAC AGCATCACCT 
CTGAATCTTG AAGACCCTAA TGTGTGTAGC CACTGGGAAA GCTACTCAGT GACTGTGCAA 
GAGTCATACC CACATCCCTT TGATCAAATT TACTACACGA GCTGCACTGA CATTCTAAAC 
TGGTTTAAAT GCACGCGGCA CAGAGTCAGC TATCGGACAG CCTATCGACA TGGGGAGAAG 
ACTATGTATA GGCGCAAGTC TCAGTGTTGT CCTGGATTTT ATGAAAGCGG GGAAATGTGT 
GTCCCCCACT GTGCTGATAA ATGTGTCCAT GGTCGCTGTA TTGCTCCAAA CACCTGTCAG 
TGTGAGCCTG GCTGGGGAGG GACCAACTGC TCCAGTGCCT GCGATGGTGA TCACTGGGGT 
CCCCACTGCA CCAGCCGGTG CCAGTGCAAA AATGGGGCTC TGTGCAACCC CATCACCGGG 
GCTTGCCACT GTGCTGCGGG CTTCCGGGGC TGGCGCTGCG AGGACCGCTG TGAGCAGGGC 
ACCTATGGTA ACGACTGTCA TCAGAGATGC CAGTGCCAGA ATGGAGCCAC CTGCGACCAC 
GTCACGGGGG AATGCCGCTG CCCACCAGGA TACACCGGAG CCTTCTGTGA GGATCTTTGT 
CCTCCTGGTA AACATGGTCC ACAGTGTGAG CAGAGATGCC CTTGTCAAAA TGGAGGAGTG 
TGTCATCACG TCACTGGAGA ATGCTCTTGC CCTTCTGGCT GGATGGGCAC AGTGTGTGGT 
CAGCCTTGCC CCGAGGGTCG CTTTGGAAAG AACTGTTCCC AAGAATGCCA GTGCCATAAT 
GGAGGGACGT GTGATGCTGC CACAGGCCAA TGTCATTGCA GTCCAGGATA CACAGGGGAA 
CGGTGCCAGG ATGAGTGTCC TGTTGGGACC TATGGCGTTC TCTGTGCTGA GACCTGCCAG 
TGTGTCAACG GAGGGAAGTG TTACCACGTG AGCGGCGCAT GCCTCTGTGA AGCAGGCTTT 
GCTGGCGAGC GCTGCGAAGC ACGCCTGTGT CCTGAGGGGC TCTACGGCAT CAAATGTGAC 
AAACGGTGTC CCTGCCACCT GGAAAACACT CATAGCTGTC ACCCCATGTC TGGAGAGTGT 
GCCTGCAAGC CGGGCTGGTC AGGACTCTAC TGTAATGAGA CATGTTCTCC TGGATTCTAC 
GGGGAAGCTT GCCAGCAGAT CTGCAGCTGC CAAAATGGGG CAGACTGTGA CAGTGTGACT 
GGAAAGTGCA CCTGTGCCCC AGGATTCAAA GGAATTGACT GCTCTACCCC ATGCCCTCTG 
GGAACCTATG GGATAAACTG TTCCTCTCGC TGTGGCTGTA AAAATGATGC AGTCTGCTCT 
CCTGTGGACG GGTCTTGTAC TTGCAAGGCA GGCTGGCACG GGGTGGACTG CTCCATCAGA 
TGTCCCAGTG GCACATGGGG CTTTGGCTGT AACTTAACAT GCCAGTGCCT CAACGGGGGA 
GCCTGCAACA CCCTGGACGG GACCTGCACG TGTGCACCTG GATGGCGCGG GGAGAAATGC 
GAACTTCCCT GCCAGGATGG CACGTACGGG CTGAACTGTG CTGAGCGCTG CGACTGCAGC 
CACGCAGATG GCTGCCACCC TACCACGGGC CATTGCCGCT GCCTCCCCGG ATGGTCAGGT 
GTCCACTGTG ACAGCGTGTG TGCTGAGGGA CGCTGGGGCC CCAACTGCTC CCTGCCCTGC 
TACTGTAAAA ATGGGGCTTC ATGCTCCCCT GATGATGGCA TCTGCGAGTG TGCACCAGGC 
TTCCGAGGCA CCACTTGTCA GAGGATCTGC TCCCCTGGTT TTTATGGGCA TCGCTGCAGC 
CAGACATGCC CACAGTGCGT TCACAGCAGC GGGCCCTGCC ACCACATCAC CGGCCTGTGT 
GACTGCTTGC CTGGCTTCAC AGGCGCCCTC TGCAATGAAG TGTGTCCCAG TGGCAGATTT 
GGGAAAAACT GTGCAGGAAT TTGTACCTGC ACCAACAACG GAACCTGTAA CCCCATTGAC 
AGATCTTGTC AGTGTTACCC CGGTTGGATT GGCAGTGACT GCTCTCAACC ATGTCCACCT 
GCCCACTGGG GCCCAAACTG CATCCACACG TGCAACTGCC ATAATGGAGC TTTCTGCAGC 
GCCTACGATG GGGAATGTAA ATGCACTCCT GGCTGGACAG GGCTCTACTG CACTCAGAGA 
TGTCCTCTAG GGTTTTATGG AAAAGATTGT GCACTGATAT GCCAATGTCA AAACGGAGCT 
GACTGCGACC ACATTTCTGG GCAGTGTACT TGCCGCACTG GATTCATGGG ACGGCACTGT 
GAGCAGAAGT GCCCTTCAGG AACATATGGC TATGGCTGTC GCCAGATATG TGATTGTCTG 
AACAACTCCA CCTGCGACCA CATCACTGGG ACCTGTTACT GCAGCCCCGG ATGGAAGGGA 
GCGAGATGTG ATCAAGCTGG TGTTATCATA GTTGGAAATC TGAACAGCTT AAGCCGAACC 
AGTACTGCTC TCCCTGCTGA TTCCTACCAG ATCGGGGCCA TTGCAGGCAT CATCATTCTT 
GTCCTAGTTG TTCTCTTCCT ACTGGCATTG TTCATTATTT ATAGACACAA GCAGAAGGGA 
AAGGAATCAA GCATGCCAGC AGTTACCTAC ACCCCTGCTA TGAGGGTCGT CAATGCAGAT 
TATACCATTT CAGGAACCCT TCCTCACAGC AATGGTGGAA ACGCTAATAG CCACTACTTC 
ACCAATCCCA GTTACCACAC GCTCACCCAG TGTGCCACAT CCCCTCACGT CAACAACAGG 
GACAGGATGA CTGTCACGAA GTCAAAAAAC AATCAACTGT TTGTGAATCT TAAAAATGTG 
AACCCTGGGA AGAGAGGCCC TGTGGGGGAC TGCACTGGGA CATTGCCGGC TGACTGGAAA 
CATGGCGGCT ACCTCAACGA GCTCGGTGCT TTTGGACTTG ACAGAAGCTA TATGGGAAAA 
TCCTTAAAAG ACCTGGGAAA GAATTCTGAA TATAATTCAA GTAACTGCTC CCTAAGCAGT 
TCTGAGAACC CATATGCCAC TATTAAAGAC CCACCTGTAC TTATCCCGAA AAGCTCAGAG 
TGTGGTTATG TGGAGATGAA ATCGCCGGCA CGAAGAGATT CCCCATATGC AGAGATCAAT 
AACTCAACTT CAGCCAACAG GAATGTCTAT GAAGTTGAAC CTACAGTGAG TGTTGTCCAA 
GGAGTATTCA GCAATAATGG GCGTCTCTCC CAGGATCCAT ATGACCTCCC AAAGAACAGT 
CACATCCCTT GTCATTATGA CCTGCTGCCA GTCCGAGACA GTTCATCCTC CCCTAAGCAA 
GAGGACAGTG GTGGTAGCAG CAGCAACAGC AGCAGCAGCA GTGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MEGF10
Entrez Gene ID	84466
Full Name	multiple EGF like domains 10
Synonyms	EMARDD
General protein information	Preferred Names multiple EGF like domains 10 Names multiple epidermal growth factor-like domains protein 10
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q23.2
Summary	This gene encodes a member of the multiple epidermal growth factor-like domains protein family. The encoded protein plays a role in cell adhesion, motility and proliferation, and is a critical mediator of apoptotic cell phagocytosis as well as amyloid-beta peptide uptake in the brain. Expression of this gene may be associated with schizophrenia, and mutations in this gene are a cause of early-onset myopathy, areflexia, respiratory distress, and dysphagia (EMARDD) as well as congenital myopathy with minicores. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Apr 2012].
Disorder MIM:	612453
Disorder Html:	Myopathy, areflexia, respiratory distress, and dysphagia, early-onset, 614399 (3); Myopathy, areflexia, respiratory distress, and dysphagia, early-onset, mild variant, 614399 (3)

Mus musculus (house mouse)	Megf10	NP_001001979.1
Danio rerio (zebrafish)	megf10	XP_001922524.2
Xenopus tropicalis (tropical clawed frog)	megf10	NP_001072726.1
Pan troglodytes (chimpanzee)	MEGF10	XP_517907.2
Bos taurus (cattle)	MEGF10	NP_001039916.2
	MEGF10	XP_001097941.1
Canis lupus familiaris (dog)	MEGF10	XP_005626533.1
Homo sapiens (human)	MEGF10	NP_115822.1
Rattus norvegicus (Norway rat)	Megf10	NP_001094127.1
Gallus gallus (chicken)	MEGF10	XP_424719.3

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MEGF10 cDNA ORF clone, Homo sapiens(human)

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gRNAs

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