ALPK1 cDNA ORF clone, Homo sapiens(human)

The following ALPK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALPK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu25440 NM_001102406.2
Latest version!
Homo sapiens alpha kinase 1 (ALPK1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu25440 NM_025144.4
Latest version!
Homo sapiens alpha kinase 1 (ALPK1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu95411 XM_017008639.1
Latest version!
Homo sapiens alpha kinase 1 (ALPK1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu95411 XM_017008638.2
Latest version!
Homo sapiens alpha kinase 1 (ALPK1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu95411 XM_017008637.1
Latest version!
Homo sapiens alpha kinase 1 (ALPK1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu95411 XM_017008640.1
Latest version!
Homo sapiens alpha kinase 1 (ALPK1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu95411 XM_017008641.2
Latest version!
Homo sapiens alpha kinase 1 (ALPK1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu95411 XM_017008634.1
Latest version!
Homo sapiens alpha kinase 1 (ALPK1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu95411 XM_017008635.1
Latest version!
Homo sapiens alpha kinase 1 (ALPK1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu95411 XM_017008633.1
Latest version!
Homo sapiens alpha kinase 1 (ALPK1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu95411 XM_017008636.1
Latest version!
Homo sapiens alpha kinase 1 (ALPK1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu95412 XM_017008642.1
Latest version!
Homo sapiens alpha kinase 1 (ALPK1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu95413 XM_017008643.1
Latest version!
Homo sapiens alpha kinase 1 (ALPK1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu95414 XM_017008644.1
Latest version!
Homo sapiens alpha kinase 1 (ALPK1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu95415 XM_017008645.1
Latest version!
Homo sapiens alpha kinase 1 (ALPK1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu95416 XM_017008646.1
Latest version!
Homo sapiens alpha kinase 1 (ALPK1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu95417 XM_017008648.1
Latest version!
Homo sapiens alpha kinase 1 (ALPK1), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu95417 XM_017008647.1
Latest version!
Homo sapiens alpha kinase 1 (ALPK1), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu95418 XM_017008649.2
Latest version!
Homo sapiens alpha kinase 1 (ALPK1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu95419 XM_017008651.1
Latest version!
Homo sapiens alpha kinase 1 (ALPK1), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu95419 XM_017008650.1
Latest version!
Homo sapiens alpha kinase 1 (ALPK1), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu14756 NM_001253884.1
Latest version!
Homo sapiens alpha kinase 1 (ALPK1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu39100 XM_005263246.4
Latest version!
Homo sapiens alpha kinase 1 (ALPK1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu39100 XM_005263245.4
Latest version!
Homo sapiens alpha kinase 1 (ALPK1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu39101 XM_006714326.3
Latest version!
Homo sapiens alpha kinase 1 (ALPK1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
View Old Accession Versions >>
OHu95418 XM_017008649.1 Homo sapiens alpha kinase 1 (ALPK1), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OHu25440 NM_001102406.1 Homo sapiens alpha kinase 1 (ALPK1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu95411 XM_017008638.1 Homo sapiens alpha kinase 1 (ALPK1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu95411 XM_017008641.1 Homo sapiens alpha kinase 1 (ALPK1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu25440 NM_025144.3 Homo sapiens alpha kinase 1 (ALPK1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu25440
    Clone ID Related Accession (Same CDS sequence) NM_025144.3 , NM_001102406.2 , NM_001102406.1 , NM_025144.4
    Accession Version NM_025144.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3735bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-01
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB250317.1, AY044164.1, BC060780.1, AK026715.1, BE155590.1 and AC004049.1. On Aug 22, 2007 this sequence version replaced NM_025144.2. Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.21906.1, SRR1660809.114154.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGAGCCT GCTGAAGGAG TTTGACCACC ATTTGCTGTC CGCTGCAGAA
    GCCTGCAAGC TGGCAGCTGC CTTCAGTGCC TATACGCCGC TCTTCGTGCT CACAGCTGTG
    AATATCCGTG GCACGTGTTT ATTGTCCTAC AGTAGTTCAA ATGACTGTCC TCCAGAATTG
    AAAAACTTAC ATCTGTGTGA AGCCAAAGAG GCCTTTGAGA TTGGCCTCCT CACCAAGAGA
    GATGATGAGC CTGTTACTGG AAAACAGGAG CTTCACAGCT TTGTCAAAGC TGCTTTCGGT
    CTCACCACAG TGCACAGAAG GCTCCATGGG GAGACAGGGA CGGTCCATGC AGCAAGTCAG
    CTCTGTAAGG AAGCAATGGG GAAGCTGTAC AATTTCAGCA CTTCCTCCAG AAGTCAGGAC
    AGAGAAGCTC TGTCTCAAGA AGTTATGTCT GTGATTGCCC AGGTGAAGGA ACATTTACAA
    GTTCAAAGCT TCTCAAATGT AGATGACAGA TCTTATGTTC CCGAGAGTTT CGAGTGCAGG
    TTGGATAAAC TTATCTTGCA TGGGCAAGGG GATTTCCAAA AAATCCTTGA CACCTATTCA
    CAGCACCATA CTTCGGTGTG TGAAGTATTT GAAAGTGATT GTGGAAACAA CAAAAATGAA
    CAGAAAGATG CAAAAACAGG AGTCTGCATC ACTGCTCTAA AAACAGAAAT AAAAAACATA
    GATACTGTGA GTACTACTCA AGAAAAGCCA CATTGTCAAA GAGACACAGG AATATCTTCC
    TCCCTAATGG GTAAGAATGT TCAGAGGGAA CTCAGAAGGG GAGGAAGGAG AAACTGGACC
    CATTCTGATG CATTTCGAGT CTCCTTGGAT CAAGATGTGG AGACTGAGAC TGAGCCATCG
    GACTACAGCA ATGGTGAGGG AGCTGTTTTC AACAAGTCTC TGAGTGGCAG CCAGACTTCC
    AGTGCTTGGA GCAACTTATC AGGGTTTAGT TCCTCTGCAA GCTGGGAGGA AGTGAATTAT
    CACGTTGACG ACAGGTCAGC CAGAAAAGAG CCTGGCAAAG AACATCTGGT GGACACTCAG
    TGTTCCACTG CCTTGTCTGA GGAGCTAGAG AATGACAGGG AAGGCAGAGC TATGCATTCA
    TTGCATTCAC AGCTTCATGA TCTCTCTCTT CAGGAACCCA ACAATGACAA TTTGGAGCCT
    TCTCAAAATC AGCCACAGCA ACAGATGCCC TTGACACCCT TCTCGCCTCA TAATACCCCA
    GGCATTTTCT TGGCCCCTGG TGCAGGGCTT CTAGAAGGAG CTCCAGAAGG TATCCAGGAA
    GTCAGAAATA TGGGACCCAG AAATACTTCT GCTCACTCCA GACCCTCATA TCGTTCTGCT
    TCTTGGTCTT CTGATTCTGG TAGGCCCAAG AATATGGGCA CACATCCTTC AGTCCAAAAA
    GAAGAAGCCT TTGAAATAAT TGTTGAGTTT CCAGAAACCA ACTGCGATGT CAAAGACAGG
    CAGGGGAAAG AGCAGGGAGA AGAAATTAGT GAAAGAGGCG CAGGCCCTAC ATTTAAAGCT
    AGTCCCTCCT GGGTTGACCC AGAAGGAGAA ACAGCAGAAA GCACTGAAGA TGCACCCTTA
    GACTTTCACA GGGTCCTGCA CAATTCTCTG GGAAACATTT CCATGCTGCC ATGTAGCTCC
    TTCACCCCTA ATTGGCCTGT TCAAAATCCT GACTCCAGAA AAAGTGGTGG CCCAGTCGCA
    GAGCAGGGCA TCGACCCTGA TGCCTCCACA GTGGATGAGG AGGGGCAACT GCTCGACAGC
    ATGGATGTTC CCTGCACAAA TGGGCACGGC TCTCATAGAC TGTGCATTCT GAGACAGCCG
    CCTGGTCAGA GGGCGGAGAC CCCCAATTCC TCTGTAAGCG GTAACATCCT CTTCCCTGTC
    CTCAGCGAGG ACTGCACTAC CACAGAGGAA GGAAATCAGC CTGGAAACAT GCTAAACTGC
    AGCCAGAACT CCAGCTCATC CTCAGTGTGG TGGCTGAAAT CACCTGCATT TTCCAGTGGT
    TCTTCTGAGG GGGACAGCCC TTGGTCCTAT CTGAATTCCA GTGGGAGTTC TTGGGTTTCA
    TTGCCGGGAA AGATGAGGAA AGAGATCCTT GAGGCTCGCA CCTTGCAACC TGATGACTTT
    GAAAAGCTGT TGGCAGGAGT GAGGCATGAT TGGCTGTTTC AGAGACTAGA GAATACGGGG
    GTTTTTAAGC CCAGTCAACT CCACCGAGCA CATAGTGCTC TTTTGTTAAA ATATTCAAAA
    AAATCTGAAC TGTGGACGGC CCAGGAAACT ATTGTCTATT TGGGGGACTA CTTGACTGTG
    AAGAAAAAAG GCAGACAAAG AAATGCTTTT TGGGTTCATC ATCTTCATCA AGAAGAAATT
    CTGGGGAGGT ATGTTGGGAA AGACTATAAG GAGCAGAAGG GGCTCTGGCA CCACTTCACT
    GATGTGGAGC GACAGATGAC CGCACAGCAC TATGTGACAG AATTTAACAA GAGACTCTAT
    GAACAAAACA TTCCCACCCA GATATTCTAC ATCCCATCCA CAATACTACT GATTTTAGAG
    GACAAGACAA TAAAGGGATG TATCAGTGTG GAGCCTTACA TACTGGGAGA ATTTGTAAAA
    TTGTCAAATA ACACGAAAGT GGTGAAAACA GAATACAAAG CCACAGAATA TGGCTTGGCC
    TATGGCCATT TTTCTTATGA GTTTTCTAAT CATAGAGATG TTGTGGTCGA TTTACAAGGT
    TGGGTAACCG GTAATGGAAA AGGACTCATC TACCTCACAG ATCCCCAGAT TCACTCCGTT
    GATCAGAAAG TTTTCACTAC CAATTTTGGA AAGAGAGGAA TTTTTTACTT CTTTAATAAC
    CAGCATGTGG AATGTAATGA AATCTGCCAT CGTCTTTCTT TGACTAGACC TTCAATGGAG
    AAACCATGCA CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_079420.3
    CDS280..4014
    Translation

    Target ORF information:

    RefSeq Version NM_025144.3
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_025144.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGAGCCT GCTGAAGGAG TTTGACCACC ATTTGCTGTC CGCTGCAGAA
    GCCTGCAAGC TGGCAGCTGC CTTCAGTGCC TATACGCCGC TCTTCGTGCT CACAGCTGTG
    AATATCCGTG GCACGTGTTT ATTGTCCTAC AGTAGTTCAA ATGACTGTCC TCCAGAATTG
    AAAAACTTAC ATCTGTGTGA AGCCAAAGAG GCCTTTGAGA TTGGCCTCCT CACCAAGAGA
    GATGATGAGC CTGTTACTGG AAAACAGGAG CTTCACAGCT TTGTCAAAGC TGCTTTCGGT
    CTCACCACAG TGCACAGAAG GCTCCATGGG GAGACAGGGA CGGTCCATGC AGCAAGTCAG
    CTCTGTAAGG AAGCAATGGG GAAGCTGTAC AATTTCAGCA CTTCCTCCAG AAGTCAGGAC
    AGAGAAGCTC TGTCTCAAGA AGTTATGTCT GTGATTGCCC AGGTGAAGGA ACATTTACAA
    GTTCAAAGCT TCTCAAATGT AGATGACAGA TCTTATGTTC CCGAGAGTTT CGAGTGCAGG
    TTGGATAAAC TTATCTTGCA TGGGCAAGGG GATTTCCAAA AAATCCTTGA CACCTATTCA
    CAGCACCATA CTTCGGTGTG TGAAGTATTT GAAAGTGATT GTGGAAACAA CAAAAATGAA
    CAGAAAGATG CAAAAACAGG AGTCTGCATC ACTGCTCTAA AAACAGAAAT AAAAAACATA
    GATACTGTGA GTACTACTCA AGAAAAGCCA CATTGTCAAA GAGACACAGG AATATCTTCC
    TCCCTAATGG GTAAGAATGT TCAGAGGGAA CTCAGAAGGG GAGGAAGGAG AAACTGGACC
    CATTCTGATG CATTTCGAGT CTCCTTGGAT CAAGATGTGG AGACTGAGAC TGAGCCATCG
    GACTACAGCA ATGGTGAGGG AGCTGTTTTC AACAAGTCTC TGAGTGGCAG CCAGACTTCC
    AGTGCTTGGA GCAACTTATC AGGGTTTAGT TCCTCTGCAA GCTGGGAGGA AGTGAATTAT
    CACGTTGACG ACAGGTCAGC CAGAAAAGAG CCTGGCAAAG AACATCTGGT GGACACTCAG
    TGTTCCACTG CCTTGTCTGA GGAGCTAGAG AATGACAGGG AAGGCAGAGC TATGCATTCA
    TTGCATTCAC AGCTTCATGA TCTCTCTCTT CAGGAACCCA ACAATGACAA TTTGGAGCCT
    TCTCAAAATC AGCCACAGCA ACAGATGCCC TTGACACCCT TCTCGCCTCA TAATACCCCA
    GGCATTTTCT TGGCCCCTGG TGCAGGGCTT CTAGAAGGAG CTCCAGAAGG TATCCAGGAA
    GTCAGAAATA TGGGACCCAG AAATACTTCT GCTCACTCCA GACCCTCATA TCGTTCTGCT
    TCTTGGTCTT CTGATTCTGG TAGGCCCAAG AATATGGGCA CACATCCTTC AGTCCAAAAA
    GAAGAAGCCT TTGAAATAAT TGTTGAGTTT CCAGAAACCA ACTGCGATGT CAAAGACAGG
    CAGGGGAAAG AGCAGGGAGA AGAAATTAGT GAAAGAGGCG CAGGCCCTAC ATTTAAAGCT
    AGTCCCTCCT GGGTTGACCC AGAAGGAGAA ACAGCAGAAA GCACTGAAGA TGCACCCTTA
    GACTTTCACA GGGTCCTGCA CAATTCTCTG GGAAACATTT CCATGCTGCC ATGTAGCTCC
    TTCACCCCTA ATTGGCCTGT TCAAAATCCT GACTCCAGAA AAAGTGGTGG CCCAGTCGCA
    GAGCAGGGCA TCGACCCTGA TGCCTCCACA GTGGATGAGG AGGGGCAACT GCTCGACAGC
    ATGGATGTTC CCTGCACAAA TGGGCACGGC TCTCATAGAC TGTGCATTCT GAGACAGCCG
    CCTGGTCAGA GGGCGGAGAC CCCCAATTCC TCTGTAAGCG GTAACATCCT CTTCCCTGTC
    CTCAGCGAGG ACTGCACTAC CACAGAGGAA GGAAATCAGC CTGGAAACAT GCTAAACTGC
    AGCCAGAACT CCAGCTCATC CTCAGTGTGG TGGCTGAAAT CACCTGCATT TTCCAGTGGT
    TCTTCTGAGG GGGACAGCCC TTGGTCCTAT CTGAATTCCA GTGGGAGTTC TTGGGTTTCA
    TTGCCGGGAA AGATGAGGAA AGAGATCCTT GAGGCTCGCA CCTTGCAACC TGATGACTTT
    GAAAAGCTGT TGGCAGGAGT GAGGCATGAT TGGCTGTTTC AGAGACTAGA GAATACGGGG
    GTTTTTAAGC CCAGTCAACT CCACCGAGCA CATAGTGCTC TTTTGTTAAA ATATTCAAAA
    AAATCTGAAC TGTGGACGGC CCAGGAAACT ATTGTCTATT TGGGGGACTA CTTGACTGTG
    AAGAAAAAAG GCAGACAAAG AAATGCTTTT TGGGTTCATC ATCTTCATCA AGAAGAAATT
    CTGGGGAGGT ATGTTGGGAA AGACTATAAG GAGCAGAAGG GGCTCTGGCA CCACTTCACT
    GATGTGGAGC GACAGATGAC CGCACAGCAC TATGTGACAG AATTTAACAA GAGACTCTAT
    GAACAAAACA TTCCCACCCA GATATTCTAC ATCCCATCCA CAATACTACT GATTTTAGAG
    GACAAGACAA TAAAGGGATG TATCAGTGTG GAGCCTTACA TACTGGGAGA ATTTGTAAAA
    TTGTCAAATA ACACGAAAGT GGTGAAAACA GAATACAAAG CCACAGAATA TGGCTTGGCC
    TATGGCCATT TTTCTTATGA GTTTTCTAAT CATAGAGATG TTGTGGTCGA TTTACAAGGT
    TGGGTAACCG GTAATGGAAA AGGACTCATC TACCTCACAG ATCCCCAGAT TCACTCCGTT
    GATCAGAAAG TTTTCACTAC CAATTTTGGA AAGAGAGGAA TTTTTTACTT CTTTAATAAC
    CAGCATGTGG AATGTAATGA AATCTGCCAT CGTCTTTCTT TGACTAGACC TTCAATGGAG
    AAACCATGCA CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25440
    Clone ID Related Accession (Same CDS sequence) NM_025144.3 , NM_001102406.2 , NM_001102406.1 , NM_025144.4
    Accession Version NM_001102406.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3735bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-27
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB250317.1, BC060780.1, AY044164.1, AK026715.1, BE155590.1 and AC004049.1. On May 31, 2019 this sequence version replaced NM_001102406.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. Variants 1 and 2 encode the same protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC060780.1, BX648798.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGAGCCT GCTGAAGGAG TTTGACCACC ATTTGCTGTC CGCTGCAGAA
    GCCTGCAAGC TGGCAGCTGC CTTCAGTGCC TATACGCCGC TCTTCGTGCT CACAGCTGTG
    AATATCCGTG GCACGTGTTT ATTGTCCTAC AGTAGTTCAA ATGACTGTCC TCCAGAATTG
    AAAAACTTAC ATCTGTGTGA AGCCAAAGAG GCCTTTGAGA TTGGCCTCCT CACCAAGAGA
    GATGATGAGC CTGTTACTGG AAAACAGGAG CTTCACAGCT TTGTCAAAGC TGCTTTCGGT
    CTCACCACAG TGCACAGAAG GCTCCATGGG GAGACAGGGA CGGTCCATGC AGCAAGTCAG
    CTCTGTAAGG AAGCAATGGG GAAGCTGTAC AATTTCAGCA CTTCCTCCAG AAGTCAGGAC
    AGAGAAGCTC TGTCTCAAGA AGTTATGTCT GTGATTGCCC AGGTGAAGGA ACATTTACAA
    GTTCAAAGCT TCTCAAATGT AGATGACAGA TCTTATGTTC CCGAGAGTTT CGAGTGCAGG
    TTGGATAAAC TTATCTTGCA TGGGCAAGGG GATTTCCAAA AAATCCTTGA CACCTATTCA
    CAGCACCATA CTTCGGTGTG TGAAGTATTT GAAAGTGATT GTGGAAACAA CAAAAATGAA
    CAGAAAGATG CAAAAACAGG AGTCTGCATC ACTGCTCTAA AAACAGAAAT AAAAAACATA
    GATACTGTGA GTACTACTCA AGAAAAGCCA CATTGTCAAA GAGACACAGG AATATCTTCC
    TCCCTAATGG GTAAGAATGT TCAGAGGGAA CTCAGAAGGG GAGGAAGGAG AAACTGGACC
    CATTCTGATG CATTTCGAGT CTCCTTGGAT CAAGATGTGG AGACTGAGAC TGAGCCATCG
    GACTACAGCA ATGGTGAGGG AGCTGTTTTC AACAAGTCTC TGAGTGGCAG CCAGACTTCC
    AGTGCTTGGA GCAACTTATC AGGGTTTAGT TCCTCTGCAA GCTGGGAGGA AGTGAATTAT
    CACGTTGACG ACAGGTCAGC CAGAAAAGAG CCTGGCAAAG AACATCTGGT GGACACTCAG
    TGTTCCACTG CCTTGTCTGA GGAGCTAGAG AATGACAGGG AAGGCAGAGC TATGCATTCA
    TTGCATTCAC AGCTTCATGA TCTCTCTCTT CAGGAACCCA ACAATGACAA TTTGGAGCCT
    TCTCAAAATC AGCCACAGCA ACAGATGCCC TTGACACCCT TCTCGCCTCA TAATACCCCA
    GGCATTTTCT TGGCCCCTGG TGCAGGGCTT CTAGAAGGAG CTCCAGAAGG TATCCAGGAA
    GTCAGAAATA TGGGACCCAG AAATACTTCT GCTCACTCCA GACCCTCATA TCGTTCTGCT
    TCTTGGTCTT CTGATTCTGG TAGGCCCAAG AATATGGGCA CACATCCTTC AGTCCAAAAA
    GAAGAAGCCT TTGAAATAAT TGTTGAGTTT CCAGAAACCA ACTGCGATGT CAAAGACAGG
    CAGGGGAAAG AGCAGGGAGA AGAAATTAGT GAAAGAGGCG CAGGCCCTAC ATTTAAAGCT
    AGTCCCTCCT GGGTTGACCC AGAAGGAGAA ACAGCAGAAA GCACTGAAGA TGCACCCTTA
    GACTTTCACA GGGTCCTGCA CAATTCTCTG GGAAACATTT CCATGCTGCC ATGTAGCTCC
    TTCACCCCTA ATTGGCCTGT TCAAAATCCT GACTCCAGAA AAAGTGGTGG CCCAGTCGCA
    GAGCAGGGCA TCGACCCTGA TGCCTCCACA GTGGATGAGG AGGGGCAACT GCTCGACAGC
    ATGGATGTTC CCTGCACAAA TGGGCACGGC TCTCATAGAC TGTGCATTCT GAGACAGCCG
    CCTGGTCAGA GGGCGGAGAC CCCCAATTCC TCTGTAAGCG GTAACATCCT CTTCCCTGTC
    CTCAGCGAGG ACTGCACTAC CACAGAGGAA GGAAATCAGC CTGGAAACAT GCTAAACTGC
    AGCCAGAACT CCAGCTCATC CTCAGTGTGG TGGCTGAAAT CACCTGCATT TTCCAGTGGT
    TCTTCTGAGG GGGACAGCCC TTGGTCCTAT CTGAATTCCA GTGGGAGTTC TTGGGTTTCA
    TTGCCGGGAA AGATGAGGAA AGAGATCCTT GAGGCTCGCA CCTTGCAACC TGATGACTTT
    GAAAAGCTGT TGGCAGGAGT GAGGCATGAT TGGCTGTTTC AGAGACTAGA GAATACGGGG
    GTTTTTAAGC CCAGTCAACT CCACCGAGCA CATAGTGCTC TTTTGTTAAA ATATTCAAAA
    AAATCTGAAC TGTGGACGGC CCAGGAAACT ATTGTCTATT TGGGGGACTA CTTGACTGTG
    AAGAAAAAAG GCAGACAAAG AAATGCTTTT TGGGTTCATC ATCTTCATCA AGAAGAAATT
    CTGGGGAGGT ATGTTGGGAA AGACTATAAG GAGCAGAAGG GGCTCTGGCA CCACTTCACT
    GATGTGGAGC GACAGATGAC CGCACAGCAC TATGTGACAG AATTTAACAA GAGACTCTAT
    GAACAAAACA TTCCCACCCA GATATTCTAC ATCCCATCCA CAATACTACT GATTTTAGAG
    GACAAGACAA TAAAGGGATG TATCAGTGTG GAGCCTTACA TACTGGGAGA ATTTGTAAAA
    TTGTCAAATA ACACGAAAGT GGTGAAAACA GAATACAAAG CCACAGAATA TGGCTTGGCC
    TATGGCCATT TTTCTTATGA GTTTTCTAAT CATAGAGATG TTGTGGTCGA TTTACAAGGT
    TGGGTAACCG GTAATGGAAA AGGACTCATC TACCTCACAG ATCCCCAGAT TCACTCCGTT
    GATCAGAAAG TTTTCACTAC CAATTTTGGA AAGAGAGGAA TTTTTTACTT CTTTAATAAC
    CAGCATGTGG AATGTAATGA AATCTGCCAT CGTCTTTCTT TGACTAGACC TTCAATGGAG
    AAACCATGCA CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001095876.1
    CDS202..3936
    Translation

    Target ORF information:

    RefSeq Version NM_001102406.2
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001102406.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGAGCCT GCTGAAGGAG TTTGACCACC ATTTGCTGTC CGCTGCAGAA
    GCCTGCAAGC TGGCAGCTGC CTTCAGTGCC TATACGCCGC TCTTCGTGCT CACAGCTGTG
    AATATCCGTG GCACGTGTTT ATTGTCCTAC AGTAGTTCAA ATGACTGTCC TCCAGAATTG
    AAAAACTTAC ATCTGTGTGA AGCCAAAGAG GCCTTTGAGA TTGGCCTCCT CACCAAGAGA
    GATGATGAGC CTGTTACTGG AAAACAGGAG CTTCACAGCT TTGTCAAAGC TGCTTTCGGT
    CTCACCACAG TGCACAGAAG GCTCCATGGG GAGACAGGGA CGGTCCATGC AGCAAGTCAG
    CTCTGTAAGG AAGCAATGGG GAAGCTGTAC AATTTCAGCA CTTCCTCCAG AAGTCAGGAC
    AGAGAAGCTC TGTCTCAAGA AGTTATGTCT GTGATTGCCC AGGTGAAGGA ACATTTACAA
    GTTCAAAGCT TCTCAAATGT AGATGACAGA TCTTATGTTC CCGAGAGTTT CGAGTGCAGG
    TTGGATAAAC TTATCTTGCA TGGGCAAGGG GATTTCCAAA AAATCCTTGA CACCTATTCA
    CAGCACCATA CTTCGGTGTG TGAAGTATTT GAAAGTGATT GTGGAAACAA CAAAAATGAA
    CAGAAAGATG CAAAAACAGG AGTCTGCATC ACTGCTCTAA AAACAGAAAT AAAAAACATA
    GATACTGTGA GTACTACTCA AGAAAAGCCA CATTGTCAAA GAGACACAGG AATATCTTCC
    TCCCTAATGG GTAAGAATGT TCAGAGGGAA CTCAGAAGGG GAGGAAGGAG AAACTGGACC
    CATTCTGATG CATTTCGAGT CTCCTTGGAT CAAGATGTGG AGACTGAGAC TGAGCCATCG
    GACTACAGCA ATGGTGAGGG AGCTGTTTTC AACAAGTCTC TGAGTGGCAG CCAGACTTCC
    AGTGCTTGGA GCAACTTATC AGGGTTTAGT TCCTCTGCAA GCTGGGAGGA AGTGAATTAT
    CACGTTGACG ACAGGTCAGC CAGAAAAGAG CCTGGCAAAG AACATCTGGT GGACACTCAG
    TGTTCCACTG CCTTGTCTGA GGAGCTAGAG AATGACAGGG AAGGCAGAGC TATGCATTCA
    TTGCATTCAC AGCTTCATGA TCTCTCTCTT CAGGAACCCA ACAATGACAA TTTGGAGCCT
    TCTCAAAATC AGCCACAGCA ACAGATGCCC TTGACACCCT TCTCGCCTCA TAATACCCCA
    GGCATTTTCT TGGCCCCTGG TGCAGGGCTT CTAGAAGGAG CTCCAGAAGG TATCCAGGAA
    GTCAGAAATA TGGGACCCAG AAATACTTCT GCTCACTCCA GACCCTCATA TCGTTCTGCT
    TCTTGGTCTT CTGATTCTGG TAGGCCCAAG AATATGGGCA CACATCCTTC AGTCCAAAAA
    GAAGAAGCCT TTGAAATAAT TGTTGAGTTT CCAGAAACCA ACTGCGATGT CAAAGACAGG
    CAGGGGAAAG AGCAGGGAGA AGAAATTAGT GAAAGAGGCG CAGGCCCTAC ATTTAAAGCT
    AGTCCCTCCT GGGTTGACCC AGAAGGAGAA ACAGCAGAAA GCACTGAAGA TGCACCCTTA
    GACTTTCACA GGGTCCTGCA CAATTCTCTG GGAAACATTT CCATGCTGCC ATGTAGCTCC
    TTCACCCCTA ATTGGCCTGT TCAAAATCCT GACTCCAGAA AAAGTGGTGG CCCAGTCGCA
    GAGCAGGGCA TCGACCCTGA TGCCTCCACA GTGGATGAGG AGGGGCAACT GCTCGACAGC
    ATGGATGTTC CCTGCACAAA TGGGCACGGC TCTCATAGAC TGTGCATTCT GAGACAGCCG
    CCTGGTCAGA GGGCGGAGAC CCCCAATTCC TCTGTAAGCG GTAACATCCT CTTCCCTGTC
    CTCAGCGAGG ACTGCACTAC CACAGAGGAA GGAAATCAGC CTGGAAACAT GCTAAACTGC
    AGCCAGAACT CCAGCTCATC CTCAGTGTGG TGGCTGAAAT CACCTGCATT TTCCAGTGGT
    TCTTCTGAGG GGGACAGCCC TTGGTCCTAT CTGAATTCCA GTGGGAGTTC TTGGGTTTCA
    TTGCCGGGAA AGATGAGGAA AGAGATCCTT GAGGCTCGCA CCTTGCAACC TGATGACTTT
    GAAAAGCTGT TGGCAGGAGT GAGGCATGAT TGGCTGTTTC AGAGACTAGA GAATACGGGG
    GTTTTTAAGC CCAGTCAACT CCACCGAGCA CATAGTGCTC TTTTGTTAAA ATATTCAAAA
    AAATCTGAAC TGTGGACGGC CCAGGAAACT ATTGTCTATT TGGGGGACTA CTTGACTGTG
    AAGAAAAAAG GCAGACAAAG AAATGCTTTT TGGGTTCATC ATCTTCATCA AGAAGAAATT
    CTGGGGAGGT ATGTTGGGAA AGACTATAAG GAGCAGAAGG GGCTCTGGCA CCACTTCACT
    GATGTGGAGC GACAGATGAC CGCACAGCAC TATGTGACAG AATTTAACAA GAGACTCTAT
    GAACAAAACA TTCCCACCCA GATATTCTAC ATCCCATCCA CAATACTACT GATTTTAGAG
    GACAAGACAA TAAAGGGATG TATCAGTGTG GAGCCTTACA TACTGGGAGA ATTTGTAAAA
    TTGTCAAATA ACACGAAAGT GGTGAAAACA GAATACAAAG CCACAGAATA TGGCTTGGCC
    TATGGCCATT TTTCTTATGA GTTTTCTAAT CATAGAGATG TTGTGGTCGA TTTACAAGGT
    TGGGTAACCG GTAATGGAAA AGGACTCATC TACCTCACAG ATCCCCAGAT TCACTCCGTT
    GATCAGAAAG TTTTCACTAC CAATTTTGGA AAGAGAGGAA TTTTTTACTT CTTTAATAAC
    CAGCATGTGG AATGTAATGA AATCTGCCAT CGTCTTTCTT TGACTAGACC TTCAATGGAG
    AAACCATGCA CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25440
    Clone ID Related Accession (Same CDS sequence) NM_025144.3 , NM_001102406.2 , NM_001102406.1 , NM_025144.4
    Accession Version NM_001102406.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3735bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-10-20
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB250317.1, BC060780.1, AY044164.1, AK026715.1, BE155590.1 and AC004049.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. Variants 1 and 2 encode the same protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX648798.1, BC060780.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGAGCCT GCTGAAGGAG TTTGACCACC ATTTGCTGTC CGCTGCAGAA
    GCCTGCAAGC TGGCAGCTGC CTTCAGTGCC TATACGCCGC TCTTCGTGCT CACAGCTGTG
    AATATCCGTG GCACGTGTTT ATTGTCCTAC AGTAGTTCAA ATGACTGTCC TCCAGAATTG
    AAAAACTTAC ATCTGTGTGA AGCCAAAGAG GCCTTTGAGA TTGGCCTCCT CACCAAGAGA
    GATGATGAGC CTGTTACTGG AAAACAGGAG CTTCACAGCT TTGTCAAAGC TGCTTTCGGT
    CTCACCACAG TGCACAGAAG GCTCCATGGG GAGACAGGGA CGGTCCATGC AGCAAGTCAG
    CTCTGTAAGG AAGCAATGGG GAAGCTGTAC AATTTCAGCA CTTCCTCCAG AAGTCAGGAC
    AGAGAAGCTC TGTCTCAAGA AGTTATGTCT GTGATTGCCC AGGTGAAGGA ACATTTACAA
    GTTCAAAGCT TCTCAAATGT AGATGACAGA TCTTATGTTC CCGAGAGTTT CGAGTGCAGG
    TTGGATAAAC TTATCTTGCA TGGGCAAGGG GATTTCCAAA AAATCCTTGA CACCTATTCA
    CAGCACCATA CTTCGGTGTG TGAAGTATTT GAAAGTGATT GTGGAAACAA CAAAAATGAA
    CAGAAAGATG CAAAAACAGG AGTCTGCATC ACTGCTCTAA AAACAGAAAT AAAAAACATA
    GATACTGTGA GTACTACTCA AGAAAAGCCA CATTGTCAAA GAGACACAGG AATATCTTCC
    TCCCTAATGG GTAAGAATGT TCAGAGGGAA CTCAGAAGGG GAGGAAGGAG AAACTGGACC
    CATTCTGATG CATTTCGAGT CTCCTTGGAT CAAGATGTGG AGACTGAGAC TGAGCCATCG
    GACTACAGCA ATGGTGAGGG AGCTGTTTTC AACAAGTCTC TGAGTGGCAG CCAGACTTCC
    AGTGCTTGGA GCAACTTATC AGGGTTTAGT TCCTCTGCAA GCTGGGAGGA AGTGAATTAT
    CACGTTGACG ACAGGTCAGC CAGAAAAGAG CCTGGCAAAG AACATCTGGT GGACACTCAG
    TGTTCCACTG CCTTGTCTGA GGAGCTAGAG AATGACAGGG AAGGCAGAGC TATGCATTCA
    TTGCATTCAC AGCTTCATGA TCTCTCTCTT CAGGAACCCA ACAATGACAA TTTGGAGCCT
    TCTCAAAATC AGCCACAGCA ACAGATGCCC TTGACACCCT TCTCGCCTCA TAATACCCCA
    GGCATTTTCT TGGCCCCTGG TGCAGGGCTT CTAGAAGGAG CTCCAGAAGG TATCCAGGAA
    GTCAGAAATA TGGGACCCAG AAATACTTCT GCTCACTCCA GACCCTCATA TCGTTCTGCT
    TCTTGGTCTT CTGATTCTGG TAGGCCCAAG AATATGGGCA CACATCCTTC AGTCCAAAAA
    GAAGAAGCCT TTGAAATAAT TGTTGAGTTT CCAGAAACCA ACTGCGATGT CAAAGACAGG
    CAGGGGAAAG AGCAGGGAGA AGAAATTAGT GAAAGAGGCG CAGGCCCTAC ATTTAAAGCT
    AGTCCCTCCT GGGTTGACCC AGAAGGAGAA ACAGCAGAAA GCACTGAAGA TGCACCCTTA
    GACTTTCACA GGGTCCTGCA CAATTCTCTG GGAAACATTT CCATGCTGCC ATGTAGCTCC
    TTCACCCCTA ATTGGCCTGT TCAAAATCCT GACTCCAGAA AAAGTGGTGG CCCAGTCGCA
    GAGCAGGGCA TCGACCCTGA TGCCTCCACA GTGGATGAGG AGGGGCAACT GCTCGACAGC
    ATGGATGTTC CCTGCACAAA TGGGCACGGC TCTCATAGAC TGTGCATTCT GAGACAGCCG
    CCTGGTCAGA GGGCGGAGAC CCCCAATTCC TCTGTAAGCG GTAACATCCT CTTCCCTGTC
    CTCAGCGAGG ACTGCACTAC CACAGAGGAA GGAAATCAGC CTGGAAACAT GCTAAACTGC
    AGCCAGAACT CCAGCTCATC CTCAGTGTGG TGGCTGAAAT CACCTGCATT TTCCAGTGGT
    TCTTCTGAGG GGGACAGCCC TTGGTCCTAT CTGAATTCCA GTGGGAGTTC TTGGGTTTCA
    TTGCCGGGAA AGATGAGGAA AGAGATCCTT GAGGCTCGCA CCTTGCAACC TGATGACTTT
    GAAAAGCTGT TGGCAGGAGT GAGGCATGAT TGGCTGTTTC AGAGACTAGA GAATACGGGG
    GTTTTTAAGC CCAGTCAACT CCACCGAGCA CATAGTGCTC TTTTGTTAAA ATATTCAAAA
    AAATCTGAAC TGTGGACGGC CCAGGAAACT ATTGTCTATT TGGGGGACTA CTTGACTGTG
    AAGAAAAAAG GCAGACAAAG AAATGCTTTT TGGGTTCATC ATCTTCATCA AGAAGAAATT
    CTGGGGAGGT ATGTTGGGAA AGACTATAAG GAGCAGAAGG GGCTCTGGCA CCACTTCACT
    GATGTGGAGC GACAGATGAC CGCACAGCAC TATGTGACAG AATTTAACAA GAGACTCTAT
    GAACAAAACA TTCCCACCCA GATATTCTAC ATCCCATCCA CAATACTACT GATTTTAGAG
    GACAAGACAA TAAAGGGATG TATCAGTGTG GAGCCTTACA TACTGGGAGA ATTTGTAAAA
    TTGTCAAATA ACACGAAAGT GGTGAAAACA GAATACAAAG CCACAGAATA TGGCTTGGCC
    TATGGCCATT TTTCTTATGA GTTTTCTAAT CATAGAGATG TTGTGGTCGA TTTACAAGGT
    TGGGTAACCG GTAATGGAAA AGGACTCATC TACCTCACAG ATCCCCAGAT TCACTCCGTT
    GATCAGAAAG TTTTCACTAC CAATTTTGGA AAGAGAGGAA TTTTTTACTT CTTTAATAAC
    CAGCATGTGG AATGTAATGA AATCTGCCAT CGTCTTTCTT TGACTAGACC TTCAATGGAG
    AAACCATGCA CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001095876.1
    CDS228..3962
    Translation

    Target ORF information:

    RefSeq Version NM_001102406.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001102406.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGAGCCT GCTGAAGGAG TTTGACCACC ATTTGCTGTC CGCTGCAGAA
    GCCTGCAAGC TGGCAGCTGC CTTCAGTGCC TATACGCCGC TCTTCGTGCT CACAGCTGTG
    AATATCCGTG GCACGTGTTT ATTGTCCTAC AGTAGTTCAA ATGACTGTCC TCCAGAATTG
    AAAAACTTAC ATCTGTGTGA AGCCAAAGAG GCCTTTGAGA TTGGCCTCCT CACCAAGAGA
    GATGATGAGC CTGTTACTGG AAAACAGGAG CTTCACAGCT TTGTCAAAGC TGCTTTCGGT
    CTCACCACAG TGCACAGAAG GCTCCATGGG GAGACAGGGA CGGTCCATGC AGCAAGTCAG
    CTCTGTAAGG AAGCAATGGG GAAGCTGTAC AATTTCAGCA CTTCCTCCAG AAGTCAGGAC
    AGAGAAGCTC TGTCTCAAGA AGTTATGTCT GTGATTGCCC AGGTGAAGGA ACATTTACAA
    GTTCAAAGCT TCTCAAATGT AGATGACAGA TCTTATGTTC CCGAGAGTTT CGAGTGCAGG
    TTGGATAAAC TTATCTTGCA TGGGCAAGGG GATTTCCAAA AAATCCTTGA CACCTATTCA
    CAGCACCATA CTTCGGTGTG TGAAGTATTT GAAAGTGATT GTGGAAACAA CAAAAATGAA
    CAGAAAGATG CAAAAACAGG AGTCTGCATC ACTGCTCTAA AAACAGAAAT AAAAAACATA
    GATACTGTGA GTACTACTCA AGAAAAGCCA CATTGTCAAA GAGACACAGG AATATCTTCC
    TCCCTAATGG GTAAGAATGT TCAGAGGGAA CTCAGAAGGG GAGGAAGGAG AAACTGGACC
    CATTCTGATG CATTTCGAGT CTCCTTGGAT CAAGATGTGG AGACTGAGAC TGAGCCATCG
    GACTACAGCA ATGGTGAGGG AGCTGTTTTC AACAAGTCTC TGAGTGGCAG CCAGACTTCC
    AGTGCTTGGA GCAACTTATC AGGGTTTAGT TCCTCTGCAA GCTGGGAGGA AGTGAATTAT
    CACGTTGACG ACAGGTCAGC CAGAAAAGAG CCTGGCAAAG AACATCTGGT GGACACTCAG
    TGTTCCACTG CCTTGTCTGA GGAGCTAGAG AATGACAGGG AAGGCAGAGC TATGCATTCA
    TTGCATTCAC AGCTTCATGA TCTCTCTCTT CAGGAACCCA ACAATGACAA TTTGGAGCCT
    TCTCAAAATC AGCCACAGCA ACAGATGCCC TTGACACCCT TCTCGCCTCA TAATACCCCA
    GGCATTTTCT TGGCCCCTGG TGCAGGGCTT CTAGAAGGAG CTCCAGAAGG TATCCAGGAA
    GTCAGAAATA TGGGACCCAG AAATACTTCT GCTCACTCCA GACCCTCATA TCGTTCTGCT
    TCTTGGTCTT CTGATTCTGG TAGGCCCAAG AATATGGGCA CACATCCTTC AGTCCAAAAA
    GAAGAAGCCT TTGAAATAAT TGTTGAGTTT CCAGAAACCA ACTGCGATGT CAAAGACAGG
    CAGGGGAAAG AGCAGGGAGA AGAAATTAGT GAAAGAGGCG CAGGCCCTAC ATTTAAAGCT
    AGTCCCTCCT GGGTTGACCC AGAAGGAGAA ACAGCAGAAA GCACTGAAGA TGCACCCTTA
    GACTTTCACA GGGTCCTGCA CAATTCTCTG GGAAACATTT CCATGCTGCC ATGTAGCTCC
    TTCACCCCTA ATTGGCCTGT TCAAAATCCT GACTCCAGAA AAAGTGGTGG CCCAGTCGCA
    GAGCAGGGCA TCGACCCTGA TGCCTCCACA GTGGATGAGG AGGGGCAACT GCTCGACAGC
    ATGGATGTTC CCTGCACAAA TGGGCACGGC TCTCATAGAC TGTGCATTCT GAGACAGCCG
    CCTGGTCAGA GGGCGGAGAC CCCCAATTCC TCTGTAAGCG GTAACATCCT CTTCCCTGTC
    CTCAGCGAGG ACTGCACTAC CACAGAGGAA GGAAATCAGC CTGGAAACAT GCTAAACTGC
    AGCCAGAACT CCAGCTCATC CTCAGTGTGG TGGCTGAAAT CACCTGCATT TTCCAGTGGT
    TCTTCTGAGG GGGACAGCCC TTGGTCCTAT CTGAATTCCA GTGGGAGTTC TTGGGTTTCA
    TTGCCGGGAA AGATGAGGAA AGAGATCCTT GAGGCTCGCA CCTTGCAACC TGATGACTTT
    GAAAAGCTGT TGGCAGGAGT GAGGCATGAT TGGCTGTTTC AGAGACTAGA GAATACGGGG
    GTTTTTAAGC CCAGTCAACT CCACCGAGCA CATAGTGCTC TTTTGTTAAA ATATTCAAAA
    AAATCTGAAC TGTGGACGGC CCAGGAAACT ATTGTCTATT TGGGGGACTA CTTGACTGTG
    AAGAAAAAAG GCAGACAAAG AAATGCTTTT TGGGTTCATC ATCTTCATCA AGAAGAAATT
    CTGGGGAGGT ATGTTGGGAA AGACTATAAG GAGCAGAAGG GGCTCTGGCA CCACTTCACT
    GATGTGGAGC GACAGATGAC CGCACAGCAC TATGTGACAG AATTTAACAA GAGACTCTAT
    GAACAAAACA TTCCCACCCA GATATTCTAC ATCCCATCCA CAATACTACT GATTTTAGAG
    GACAAGACAA TAAAGGGATG TATCAGTGTG GAGCCTTACA TACTGGGAGA ATTTGTAAAA
    TTGTCAAATA ACACGAAAGT GGTGAAAACA GAATACAAAG CCACAGAATA TGGCTTGGCC
    TATGGCCATT TTTCTTATGA GTTTTCTAAT CATAGAGATG TTGTGGTCGA TTTACAAGGT
    TGGGTAACCG GTAATGGAAA AGGACTCATC TACCTCACAG ATCCCCAGAT TCACTCCGTT
    GATCAGAAAG TTTTCACTAC CAATTTTGGA AAGAGAGGAA TTTTTTACTT CTTTAATAAC
    CAGCATGTGG AATGTAATGA AATCTGCCAT CGTCTTTCTT TGACTAGACC TTCAATGGAG
    AAACCATGCA CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25440
    Clone ID Related Accession (Same CDS sequence) NM_025144.3 , NM_001102406.2 , NM_001102406.1 , NM_025144.4
    Accession Version NM_025144.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3735bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-27
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB250317.1, AY044164.1, BC060780.1, AK026715.1, BE155590.1 and AC004049.1. On Nov 23, 2018 this sequence version replaced NM_025144.3. Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same protein (isoform 1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.21906.1, SRR1660809.114154.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000650871.1/ ENSP00000498374.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGAGCCT GCTGAAGGAG TTTGACCACC ATTTGCTGTC CGCTGCAGAA
    GCCTGCAAGC TGGCAGCTGC CTTCAGTGCC TATACGCCGC TCTTCGTGCT CACAGCTGTG
    AATATCCGTG GCACGTGTTT ATTGTCCTAC AGTAGTTCAA ATGACTGTCC TCCAGAATTG
    AAAAACTTAC ATCTGTGTGA AGCCAAAGAG GCCTTTGAGA TTGGCCTCCT CACCAAGAGA
    GATGATGAGC CTGTTACTGG AAAACAGGAG CTTCACAGCT TTGTCAAAGC TGCTTTCGGT
    CTCACCACAG TGCACAGAAG GCTCCATGGG GAGACAGGGA CGGTCCATGC AGCAAGTCAG
    CTCTGTAAGG AAGCAATGGG GAAGCTGTAC AATTTCAGCA CTTCCTCCAG AAGTCAGGAC
    AGAGAAGCTC TGTCTCAAGA AGTTATGTCT GTGATTGCCC AGGTGAAGGA ACATTTACAA
    GTTCAAAGCT TCTCAAATGT AGATGACAGA TCTTATGTTC CCGAGAGTTT CGAGTGCAGG
    TTGGATAAAC TTATCTTGCA TGGGCAAGGG GATTTCCAAA AAATCCTTGA CACCTATTCA
    CAGCACCATA CTTCGGTGTG TGAAGTATTT GAAAGTGATT GTGGAAACAA CAAAAATGAA
    CAGAAAGATG CAAAAACAGG AGTCTGCATC ACTGCTCTAA AAACAGAAAT AAAAAACATA
    GATACTGTGA GTACTACTCA AGAAAAGCCA CATTGTCAAA GAGACACAGG AATATCTTCC
    TCCCTAATGG GTAAGAATGT TCAGAGGGAA CTCAGAAGGG GAGGAAGGAG AAACTGGACC
    CATTCTGATG CATTTCGAGT CTCCTTGGAT CAAGATGTGG AGACTGAGAC TGAGCCATCG
    GACTACAGCA ATGGTGAGGG AGCTGTTTTC AACAAGTCTC TGAGTGGCAG CCAGACTTCC
    AGTGCTTGGA GCAACTTATC AGGGTTTAGT TCCTCTGCAA GCTGGGAGGA AGTGAATTAT
    CACGTTGACG ACAGGTCAGC CAGAAAAGAG CCTGGCAAAG AACATCTGGT GGACACTCAG
    TGTTCCACTG CCTTGTCTGA GGAGCTAGAG AATGACAGGG AAGGCAGAGC TATGCATTCA
    TTGCATTCAC AGCTTCATGA TCTCTCTCTT CAGGAACCCA ACAATGACAA TTTGGAGCCT
    TCTCAAAATC AGCCACAGCA ACAGATGCCC TTGACACCCT TCTCGCCTCA TAATACCCCA
    GGCATTTTCT TGGCCCCTGG TGCAGGGCTT CTAGAAGGAG CTCCAGAAGG TATCCAGGAA
    GTCAGAAATA TGGGACCCAG AAATACTTCT GCTCACTCCA GACCCTCATA TCGTTCTGCT
    TCTTGGTCTT CTGATTCTGG TAGGCCCAAG AATATGGGCA CACATCCTTC AGTCCAAAAA
    GAAGAAGCCT TTGAAATAAT TGTTGAGTTT CCAGAAACCA ACTGCGATGT CAAAGACAGG
    CAGGGGAAAG AGCAGGGAGA AGAAATTAGT GAAAGAGGCG CAGGCCCTAC ATTTAAAGCT
    AGTCCCTCCT GGGTTGACCC AGAAGGAGAA ACAGCAGAAA GCACTGAAGA TGCACCCTTA
    GACTTTCACA GGGTCCTGCA CAATTCTCTG GGAAACATTT CCATGCTGCC ATGTAGCTCC
    TTCACCCCTA ATTGGCCTGT TCAAAATCCT GACTCCAGAA AAAGTGGTGG CCCAGTCGCA
    GAGCAGGGCA TCGACCCTGA TGCCTCCACA GTGGATGAGG AGGGGCAACT GCTCGACAGC
    ATGGATGTTC CCTGCACAAA TGGGCACGGC TCTCATAGAC TGTGCATTCT GAGACAGCCG
    CCTGGTCAGA GGGCGGAGAC CCCCAATTCC TCTGTAAGCG GTAACATCCT CTTCCCTGTC
    CTCAGCGAGG ACTGCACTAC CACAGAGGAA GGAAATCAGC CTGGAAACAT GCTAAACTGC
    AGCCAGAACT CCAGCTCATC CTCAGTGTGG TGGCTGAAAT CACCTGCATT TTCCAGTGGT
    TCTTCTGAGG GGGACAGCCC TTGGTCCTAT CTGAATTCCA GTGGGAGTTC TTGGGTTTCA
    TTGCCGGGAA AGATGAGGAA AGAGATCCTT GAGGCTCGCA CCTTGCAACC TGATGACTTT
    GAAAAGCTGT TGGCAGGAGT GAGGCATGAT TGGCTGTTTC AGAGACTAGA GAATACGGGG
    GTTTTTAAGC CCAGTCAACT CCACCGAGCA CATAGTGCTC TTTTGTTAAA ATATTCAAAA
    AAATCTGAAC TGTGGACGGC CCAGGAAACT ATTGTCTATT TGGGGGACTA CTTGACTGTG
    AAGAAAAAAG GCAGACAAAG AAATGCTTTT TGGGTTCATC ATCTTCATCA AGAAGAAATT
    CTGGGGAGGT ATGTTGGGAA AGACTATAAG GAGCAGAAGG GGCTCTGGCA CCACTTCACT
    GATGTGGAGC GACAGATGAC CGCACAGCAC TATGTGACAG AATTTAACAA GAGACTCTAT
    GAACAAAACA TTCCCACCCA GATATTCTAC ATCCCATCCA CAATACTACT GATTTTAGAG
    GACAAGACAA TAAAGGGATG TATCAGTGTG GAGCCTTACA TACTGGGAGA ATTTGTAAAA
    TTGTCAAATA ACACGAAAGT GGTGAAAACA GAATACAAAG CCACAGAATA TGGCTTGGCC
    TATGGCCATT TTTCTTATGA GTTTTCTAAT CATAGAGATG TTGTGGTCGA TTTACAAGGT
    TGGGTAACCG GTAATGGAAA AGGACTCATC TACCTCACAG ATCCCCAGAT TCACTCCGTT
    GATCAGAAAG TTTTCACTAC CAATTTTGGA AAGAGAGGAA TTTTTTACTT CTTTAATAAC
    CAGCATGTGG AATGTAATGA AATCTGCCAT CGTCTTTCTT TGACTAGACC TTCAATGGAG
    AAACCATGCA CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_079420.3
    CDS254..3988
    Translation

    Target ORF information:

    RefSeq Version NM_025144.4
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_025144.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGAGCCT GCTGAAGGAG TTTGACCACC ATTTGCTGTC CGCTGCAGAA
    GCCTGCAAGC TGGCAGCTGC CTTCAGTGCC TATACGCCGC TCTTCGTGCT CACAGCTGTG
    AATATCCGTG GCACGTGTTT ATTGTCCTAC AGTAGTTCAA ATGACTGTCC TCCAGAATTG
    AAAAACTTAC ATCTGTGTGA AGCCAAAGAG GCCTTTGAGA TTGGCCTCCT CACCAAGAGA
    GATGATGAGC CTGTTACTGG AAAACAGGAG CTTCACAGCT TTGTCAAAGC TGCTTTCGGT
    CTCACCACAG TGCACAGAAG GCTCCATGGG GAGACAGGGA CGGTCCATGC AGCAAGTCAG
    CTCTGTAAGG AAGCAATGGG GAAGCTGTAC AATTTCAGCA CTTCCTCCAG AAGTCAGGAC
    AGAGAAGCTC TGTCTCAAGA AGTTATGTCT GTGATTGCCC AGGTGAAGGA ACATTTACAA
    GTTCAAAGCT TCTCAAATGT AGATGACAGA TCTTATGTTC CCGAGAGTTT CGAGTGCAGG
    TTGGATAAAC TTATCTTGCA TGGGCAAGGG GATTTCCAAA AAATCCTTGA CACCTATTCA
    CAGCACCATA CTTCGGTGTG TGAAGTATTT GAAAGTGATT GTGGAAACAA CAAAAATGAA
    CAGAAAGATG CAAAAACAGG AGTCTGCATC ACTGCTCTAA AAACAGAAAT AAAAAACATA
    GATACTGTGA GTACTACTCA AGAAAAGCCA CATTGTCAAA GAGACACAGG AATATCTTCC
    TCCCTAATGG GTAAGAATGT TCAGAGGGAA CTCAGAAGGG GAGGAAGGAG AAACTGGACC
    CATTCTGATG CATTTCGAGT CTCCTTGGAT CAAGATGTGG AGACTGAGAC TGAGCCATCG
    GACTACAGCA ATGGTGAGGG AGCTGTTTTC AACAAGTCTC TGAGTGGCAG CCAGACTTCC
    AGTGCTTGGA GCAACTTATC AGGGTTTAGT TCCTCTGCAA GCTGGGAGGA AGTGAATTAT
    CACGTTGACG ACAGGTCAGC CAGAAAAGAG CCTGGCAAAG AACATCTGGT GGACACTCAG
    TGTTCCACTG CCTTGTCTGA GGAGCTAGAG AATGACAGGG AAGGCAGAGC TATGCATTCA
    TTGCATTCAC AGCTTCATGA TCTCTCTCTT CAGGAACCCA ACAATGACAA TTTGGAGCCT
    TCTCAAAATC AGCCACAGCA ACAGATGCCC TTGACACCCT TCTCGCCTCA TAATACCCCA
    GGCATTTTCT TGGCCCCTGG TGCAGGGCTT CTAGAAGGAG CTCCAGAAGG TATCCAGGAA
    GTCAGAAATA TGGGACCCAG AAATACTTCT GCTCACTCCA GACCCTCATA TCGTTCTGCT
    TCTTGGTCTT CTGATTCTGG TAGGCCCAAG AATATGGGCA CACATCCTTC AGTCCAAAAA
    GAAGAAGCCT TTGAAATAAT TGTTGAGTTT CCAGAAACCA ACTGCGATGT CAAAGACAGG
    CAGGGGAAAG AGCAGGGAGA AGAAATTAGT GAAAGAGGCG CAGGCCCTAC ATTTAAAGCT
    AGTCCCTCCT GGGTTGACCC AGAAGGAGAA ACAGCAGAAA GCACTGAAGA TGCACCCTTA
    GACTTTCACA GGGTCCTGCA CAATTCTCTG GGAAACATTT CCATGCTGCC ATGTAGCTCC
    TTCACCCCTA ATTGGCCTGT TCAAAATCCT GACTCCAGAA AAAGTGGTGG CCCAGTCGCA
    GAGCAGGGCA TCGACCCTGA TGCCTCCACA GTGGATGAGG AGGGGCAACT GCTCGACAGC
    ATGGATGTTC CCTGCACAAA TGGGCACGGC TCTCATAGAC TGTGCATTCT GAGACAGCCG
    CCTGGTCAGA GGGCGGAGAC CCCCAATTCC TCTGTAAGCG GTAACATCCT CTTCCCTGTC
    CTCAGCGAGG ACTGCACTAC CACAGAGGAA GGAAATCAGC CTGGAAACAT GCTAAACTGC
    AGCCAGAACT CCAGCTCATC CTCAGTGTGG TGGCTGAAAT CACCTGCATT TTCCAGTGGT
    TCTTCTGAGG GGGACAGCCC TTGGTCCTAT CTGAATTCCA GTGGGAGTTC TTGGGTTTCA
    TTGCCGGGAA AGATGAGGAA AGAGATCCTT GAGGCTCGCA CCTTGCAACC TGATGACTTT
    GAAAAGCTGT TGGCAGGAGT GAGGCATGAT TGGCTGTTTC AGAGACTAGA GAATACGGGG
    GTTTTTAAGC CCAGTCAACT CCACCGAGCA CATAGTGCTC TTTTGTTAAA ATATTCAAAA
    AAATCTGAAC TGTGGACGGC CCAGGAAACT ATTGTCTATT TGGGGGACTA CTTGACTGTG
    AAGAAAAAAG GCAGACAAAG AAATGCTTTT TGGGTTCATC ATCTTCATCA AGAAGAAATT
    CTGGGGAGGT ATGTTGGGAA AGACTATAAG GAGCAGAAGG GGCTCTGGCA CCACTTCACT
    GATGTGGAGC GACAGATGAC CGCACAGCAC TATGTGACAG AATTTAACAA GAGACTCTAT
    GAACAAAACA TTCCCACCCA GATATTCTAC ATCCCATCCA CAATACTACT GATTTTAGAG
    GACAAGACAA TAAAGGGATG TATCAGTGTG GAGCCTTACA TACTGGGAGA ATTTGTAAAA
    TTGTCAAATA ACACGAAAGT GGTGAAAACA GAATACAAAG CCACAGAATA TGGCTTGGCC
    TATGGCCATT TTTCTTATGA GTTTTCTAAT CATAGAGATG TTGTGGTCGA TTTACAAGGT
    TGGGTAACCG GTAATGGAAA AGGACTCATC TACCTCACAG ATCCCCAGAT TCACTCCGTT
    GATCAGAAAG TTTTCACTAC CAATTTTGGA AAGAGAGGAA TTTTTTACTT CTTTAATAAC
    CAGCATGTGG AATGTAATGA AATCTGCCAT CGTCTTTCTT TGACTAGACC TTCAATGGAG
    AAACCATGCA CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95411
    Clone ID Related Accession (Same CDS sequence) XM_017008638.2 , XM_017008636.1 , XM_017008635.1 , XM_017008640.1 , XM_017008638.1 , XM_017008633.1 , XM_017008634.1 , XM_017008637.1 , XM_017008639.1 , XM_017008641.2 , XM_017008641.1
    Accession Version XM_017008638.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3756bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017008638.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGCCTCC TGTTTTCTCA CAGAGCCTGC TGAAGGAGTT TGACCACCAT
    TTGCTGTCCG CTGCAGAAGC CTGCAAGCTG GCAGCTGCCT TCAGTGCCTA TACGCCGCTC
    TTCGTGCTCA CAGCTGTGAA TATCCGTGGC ACGTGTTTAT TGTCCTACAG TAGTTCAAAT
    GACTGTCCTC CAGAATTGAA AAACTTACAT CTGTGTGAAG CCAAAGAGGC CTTTGAGATT
    GGCCTCCTCA CCAAGAGAGA TGATGAGCCT GTTACTGGAA AACAGGAGCT TCACAGCTTT
    GTCAAAGCTG CTTTCGGTCT CACCACAGTG CACAGAAGGC TCCATGGGGA GACAGGGACG
    GTCCATGCAG CAAGTCAGCT CTGTAAGGAA GCAATGGGGA AGCTGTACAA TTTCAGCACT
    TCCTCCAGAA GTCAGGACAG AGAAGCTCTG TCTCAAGAAG TTATGTCTGT GATTGCCCAG
    GTGAAGGAAC ATTTACAAGT TCAAAGCTTC TCAAATGTAG ATGACAGATC TTATGTTCCC
    GAGAGTTTCG AGTGCAGGTT GGATAAACTT ATCTTGCATG GGCAAGGGGA TTTCCAAAAA
    ATCCTTGACA CCTATTCACA GCACCATACT TCGGTGTGTG AAGTATTTGA AAGTGATTGT
    GGAAACAACA AAAATGAACA GAAAGATGCA AAAACAGGAG TCTGCATCAC TGCTCTAAAA
    ACAGAAATAA AAAACATAGA TACTGTGAGT ACTACTCAAG AAAAGCCACA TTGTCAAAGA
    GACACAGGAA TATCTTCCTC CCTAATGGGT AAGAATGTTC AGAGGGAACT CAGAAGGGGA
    GGAAGGAGAA ACTGGACCCA TTCTGATGCA TTTCGAGTCT CCTTGGATCA AGATGTGGAG
    ACTGAGACTG AGCCATCGGA CTACAGCAAT GGTGAGGGAG CTGTTTTCAA CAAGTCTCTG
    AGTGGCAGCC AGACTTCCAG TGCTTGGAGC AACTTATCAG GGTTTAGTTC CTCTGCAAGC
    TGGGAGGAAG TGAATTATCA CGTTGACGAC AGGTCAGCCA GAAAAGAGCC TGGCAAAGAA
    CATCTGGTGG ACACTCAGTG TTCCACTGCC TTGTCTGAGG AGCTAGAGAA TGACAGGGAA
    GGCAGAGCTA TGCATTCATT GCATTCACAG CTTCATGATC TCTCTCTTCA GGAACCCAAC
    AATGACAATT TGGAGCCTTC TCAAAATCAG CCACAGCAAC AGATGCCCTT GACACCCTTC
    TCGCCTCATA ATACCCCAGG CATTTTCTTG GCCCCTGGTG CAGGGCTTCT AGAAGGAGCT
    CCAGAAGGTA TCCAGGAAGT CAGAAATATG GGACCCAGAA ATACTTCTGC TCACTCCAGA
    CCCTCATATC GTTCTGCTTC TTGGTCTTCT GATTCTGGTA GGCCCAAGAA TATGGGCACA
    CATCCTTCAG TCCAAAAAGA AGAAGCCTTT GAAATAATTG TTGAGTTTCC AGAAACCAAC
    TGCGATGTCA AAGACAGGCA GGGGAAAGAG CAGGGAGAAG AAATTAGTGA AAGAGGCGCA
    GGCCCTACAT TTAAAGCTAG TCCCTCCTGG GTTGACCCAG AAGGAGAAAC AGCAGAAAGC
    ACTGAAGATG CACCCTTAGA CTTTCACAGG GTCCTGCACA ATTCTCTGGG AAACATTTCC
    ATGCTGCCAT GTAGCTCCTT CACCCCTAAT TGGCCTGTTC AAAATCCTGA CTCCAGAAAA
    AGTGGTGGCC CAGTCGCAGA GCAGGGCATC GACCCTGATG CCTCCACAGT GGATGAGGAG
    GGGCAACTGC TCGACAGCAT GGATGTTCCC TGCACAAATG GGCACGGCTC TCATAGACTG
    TGCATTCTGA GACAGCCGCC TGGTCAGAGG GCGGAGACCC CCAATTCCTC TGTAAGCGGT
    AACATCCTCT TCCCTGTCCT CAGCGAGGAC TGCACTACCA CAGAGGAAGG AAATCAGCCT
    GGAAACATGC TAAACTGCAG CCAGAACTCC AGCTCATCCT CAGTGTGGTG GCTGAAATCA
    CCTGCATTTT CCAGTGGTTC TTCTGAGGGG GACAGCCCTT GGTCCTATCT GAATTCCAGT
    GGGAGTTCTT GGGTTTCATT GCCGGGAAAG ATGAGGAAAG AGATCCTTGA GGCTCGCACC
    TTGCAACCTG ATGACTTTGA AAAGCTGTTG GCAGGAGTGA GGCATGATTG GCTGTTTCAG
    AGACTAGAGA ATACGGGGGT TTTTAAGCCC AGTCAACTCC ACCGAGCACA TAGTGCTCTT
    TTGTTAAAAT ATTCAAAAAA ATCTGAACTG TGGACGGCCC AGGAAACTAT TGTCTATTTG
    GGGGACTACT TGACTGTGAA GAAAAAAGGC AGACAAAGAA ATGCTTTTTG GGTTCATCAT
    CTTCATCAAG AAGAAATTCT GGGGAGGTAT GTTGGGAAAG ACTATAAGGA GCAGAAGGGG
    CTCTGGCACC ACTTCACTGA TGTGGAGCGA CAGATGACCG CACAGCACTA TGTGACAGAA
    TTTAACAAGA GACTCTATGA ACAAAACATT CCCACCCAGA TATTCTACAT CCCATCCACA
    ATACTACTGA TTTTAGAGGA CAAGACAATA AAGGGATGTA TCAGTGTGGA GCCTTACATA
    CTGGGAGAAT TTGTAAAATT GTCAAATAAC ACGAAAGTGG TGAAAACAGA ATACAAAGCC
    ACAGAATATG GCTTGGCCTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT
    GTGGTCGATT TACAAGGTTG GGTAACCGGT AATGGAAAAG GACTCATCTA CCTCACAGAT
    CCCCAGATTC ACTCCGTTGA TCAGAAAGTT TTCACTACCA ATTTTGGAAA GAGAGGAATT
    TTTTACTTCT TTAATAACCA GCATGTGGAA TGTAATGAAA TCTGCCATCG TCTTTCTTTG
    ACTAGACCTT CAATGGAGAA ACCATGTAAG TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864127.1
    CDS794..4549
    Translation

    Target ORF information:

    RefSeq Version XM_017008638.2
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008638.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGCCTCC TGTTTTCTCA CAGAGCCTGC TGAAGGAGTT TGACCACCAT
    TTGCTGTCCG CTGCAGAAGC CTGCAAGCTG GCAGCTGCCT TCAGTGCCTA TACGCCGCTC
    TTCGTGCTCA CAGCTGTGAA TATCCGTGGC ACGTGTTTAT TGTCCTACAG TAGTTCAAAT
    GACTGTCCTC CAGAATTGAA AAACTTACAT CTGTGTGAAG CCAAAGAGGC CTTTGAGATT
    GGCCTCCTCA CCAAGAGAGA TGATGAGCCT GTTACTGGAA AACAGGAGCT TCACAGCTTT
    GTCAAAGCTG CTTTCGGTCT CACCACAGTG CACAGAAGGC TCCATGGGGA GACAGGGACG
    GTCCATGCAG CAAGTCAGCT CTGTAAGGAA GCAATGGGGA AGCTGTACAA TTTCAGCACT
    TCCTCCAGAA GTCAGGACAG AGAAGCTCTG TCTCAAGAAG TTATGTCTGT GATTGCCCAG
    GTGAAGGAAC ATTTACAAGT TCAAAGCTTC TCAAATGTAG ATGACAGATC TTATGTTCCC
    GAGAGTTTCG AGTGCAGGTT GGATAAACTT ATCTTGCATG GGCAAGGGGA TTTCCAAAAA
    ATCCTTGACA CCTATTCACA GCACCATACT TCGGTGTGTG AAGTATTTGA AAGTGATTGT
    GGAAACAACA AAAATGAACA GAAAGATGCA AAAACAGGAG TCTGCATCAC TGCTCTAAAA
    ACAGAAATAA AAAACATAGA TACTGTGAGT ACTACTCAAG AAAAGCCACA TTGTCAAAGA
    GACACAGGAA TATCTTCCTC CCTAATGGGT AAGAATGTTC AGAGGGAACT CAGAAGGGGA
    GGAAGGAGAA ACTGGACCCA TTCTGATGCA TTTCGAGTCT CCTTGGATCA AGATGTGGAG
    ACTGAGACTG AGCCATCGGA CTACAGCAAT GGTGAGGGAG CTGTTTTCAA CAAGTCTCTG
    AGTGGCAGCC AGACTTCCAG TGCTTGGAGC AACTTATCAG GGTTTAGTTC CTCTGCAAGC
    TGGGAGGAAG TGAATTATCA CGTTGACGAC AGGTCAGCCA GAAAAGAGCC TGGCAAAGAA
    CATCTGGTGG ACACTCAGTG TTCCACTGCC TTGTCTGAGG AGCTAGAGAA TGACAGGGAA
    GGCAGAGCTA TGCATTCATT GCATTCACAG CTTCATGATC TCTCTCTTCA GGAACCCAAC
    AATGACAATT TGGAGCCTTC TCAAAATCAG CCACAGCAAC AGATGCCCTT GACACCCTTC
    TCGCCTCATA ATACCCCAGG CATTTTCTTG GCCCCTGGTG CAGGGCTTCT AGAAGGAGCT
    CCAGAAGGTA TCCAGGAAGT CAGAAATATG GGACCCAGAA ATACTTCTGC TCACTCCAGA
    CCCTCATATC GTTCTGCTTC TTGGTCTTCT GATTCTGGTA GGCCCAAGAA TATGGGCACA
    CATCCTTCAG TCCAAAAAGA AGAAGCCTTT GAAATAATTG TTGAGTTTCC AGAAACCAAC
    TGCGATGTCA AAGACAGGCA GGGGAAAGAG CAGGGAGAAG AAATTAGTGA AAGAGGCGCA
    GGCCCTACAT TTAAAGCTAG TCCCTCCTGG GTTGACCCAG AAGGAGAAAC AGCAGAAAGC
    ACTGAAGATG CACCCTTAGA CTTTCACAGG GTCCTGCACA ATTCTCTGGG AAACATTTCC
    ATGCTGCCAT GTAGCTCCTT CACCCCTAAT TGGCCTGTTC AAAATCCTGA CTCCAGAAAA
    AGTGGTGGCC CAGTCGCAGA GCAGGGCATC GACCCTGATG CCTCCACAGT GGATGAGGAG
    GGGCAACTGC TCGACAGCAT GGATGTTCCC TGCACAAATG GGCACGGCTC TCATAGACTG
    TGCATTCTGA GACAGCCGCC TGGTCAGAGG GCGGAGACCC CCAATTCCTC TGTAAGCGGT
    AACATCCTCT TCCCTGTCCT CAGCGAGGAC TGCACTACCA CAGAGGAAGG AAATCAGCCT
    GGAAACATGC TAAACTGCAG CCAGAACTCC AGCTCATCCT CAGTGTGGTG GCTGAAATCA
    CCTGCATTTT CCAGTGGTTC TTCTGAGGGG GACAGCCCTT GGTCCTATCT GAATTCCAGT
    GGGAGTTCTT GGGTTTCATT GCCGGGAAAG ATGAGGAAAG AGATCCTTGA GGCTCGCACC
    TTGCAACCTG ATGACTTTGA AAAGCTGTTG GCAGGAGTGA GGCATGATTG GCTGTTTCAG
    AGACTAGAGA ATACGGGGGT TTTTAAGCCC AGTCAACTCC ACCGAGCACA TAGTGCTCTT
    TTGTTAAAAT ATTCAAAAAA ATCTGAACTG TGGACGGCCC AGGAAACTAT TGTCTATTTG
    GGGGACTACT TGACTGTGAA GAAAAAAGGC AGACAAAGAA ATGCTTTTTG GGTTCATCAT
    CTTCATCAAG AAGAAATTCT GGGGAGGTAT GTTGGGAAAG ACTATAAGGA GCAGAAGGGG
    CTCTGGCACC ACTTCACTGA TGTGGAGCGA CAGATGACCG CACAGCACTA TGTGACAGAA
    TTTAACAAGA GACTCTATGA ACAAAACATT CCCACCCAGA TATTCTACAT CCCATCCACA
    ATACTACTGA TTTTAGAGGA CAAGACAATA AAGGGATGTA TCAGTGTGGA GCCTTACATA
    CTGGGAGAAT TTGTAAAATT GTCAAATAAC ACGAAAGTGG TGAAAACAGA ATACAAAGCC
    ACAGAATATG GCTTGGCCTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT
    GTGGTCGATT TACAAGGTTG GGTAACCGGT AATGGAAAAG GACTCATCTA CCTCACAGAT
    CCCCAGATTC ACTCCGTTGA TCAGAAAGTT TTCACTACCA ATTTTGGAAA GAGAGGAATT
    TTTTACTTCT TTAATAACCA GCATGTGGAA TGTAATGAAA TCTGCCATCG TCTTTCTTTG
    ACTAGACCTT CAATGGAGAA ACCATGTAAG TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95411
    Clone ID Related Accession (Same CDS sequence) XM_017008638.2 , XM_017008636.1 , XM_017008635.1 , XM_017008640.1 , XM_017008638.1 , XM_017008633.1 , XM_017008634.1 , XM_017008637.1 , XM_017008639.1 , XM_017008641.2 , XM_017008641.1
    Accession Version XM_017008636.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3756bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGCCTCC TGTTTTCTCA CAGAGCCTGC TGAAGGAGTT TGACCACCAT
    TTGCTGTCCG CTGCAGAAGC CTGCAAGCTG GCAGCTGCCT TCAGTGCCTA TACGCCGCTC
    TTCGTGCTCA CAGCTGTGAA TATCCGTGGC ACGTGTTTAT TGTCCTACAG TAGTTCAAAT
    GACTGTCCTC CAGAATTGAA AAACTTACAT CTGTGTGAAG CCAAAGAGGC CTTTGAGATT
    GGCCTCCTCA CCAAGAGAGA TGATGAGCCT GTTACTGGAA AACAGGAGCT TCACAGCTTT
    GTCAAAGCTG CTTTCGGTCT CACCACAGTG CACAGAAGGC TCCATGGGGA GACAGGGACG
    GTCCATGCAG CAAGTCAGCT CTGTAAGGAA GCAATGGGGA AGCTGTACAA TTTCAGCACT
    TCCTCCAGAA GTCAGGACAG AGAAGCTCTG TCTCAAGAAG TTATGTCTGT GATTGCCCAG
    GTGAAGGAAC ATTTACAAGT TCAAAGCTTC TCAAATGTAG ATGACAGATC TTATGTTCCC
    GAGAGTTTCG AGTGCAGGTT GGATAAACTT ATCTTGCATG GGCAAGGGGA TTTCCAAAAA
    ATCCTTGACA CCTATTCACA GCACCATACT TCGGTGTGTG AAGTATTTGA AAGTGATTGT
    GGAAACAACA AAAATGAACA GAAAGATGCA AAAACAGGAG TCTGCATCAC TGCTCTAAAA
    ACAGAAATAA AAAACATAGA TACTGTGAGT ACTACTCAAG AAAAGCCACA TTGTCAAAGA
    GACACAGGAA TATCTTCCTC CCTAATGGGT AAGAATGTTC AGAGGGAACT CAGAAGGGGA
    GGAAGGAGAA ACTGGACCCA TTCTGATGCA TTTCGAGTCT CCTTGGATCA AGATGTGGAG
    ACTGAGACTG AGCCATCGGA CTACAGCAAT GGTGAGGGAG CTGTTTTCAA CAAGTCTCTG
    AGTGGCAGCC AGACTTCCAG TGCTTGGAGC AACTTATCAG GGTTTAGTTC CTCTGCAAGC
    TGGGAGGAAG TGAATTATCA CGTTGACGAC AGGTCAGCCA GAAAAGAGCC TGGCAAAGAA
    CATCTGGTGG ACACTCAGTG TTCCACTGCC TTGTCTGAGG AGCTAGAGAA TGACAGGGAA
    GGCAGAGCTA TGCATTCATT GCATTCACAG CTTCATGATC TCTCTCTTCA GGAACCCAAC
    AATGACAATT TGGAGCCTTC TCAAAATCAG CCACAGCAAC AGATGCCCTT GACACCCTTC
    TCGCCTCATA ATACCCCAGG CATTTTCTTG GCCCCTGGTG CAGGGCTTCT AGAAGGAGCT
    CCAGAAGGTA TCCAGGAAGT CAGAAATATG GGACCCAGAA ATACTTCTGC TCACTCCAGA
    CCCTCATATC GTTCTGCTTC TTGGTCTTCT GATTCTGGTA GGCCCAAGAA TATGGGCACA
    CATCCTTCAG TCCAAAAAGA AGAAGCCTTT GAAATAATTG TTGAGTTTCC AGAAACCAAC
    TGCGATGTCA AAGACAGGCA GGGGAAAGAG CAGGGAGAAG AAATTAGTGA AAGAGGCGCA
    GGCCCTACAT TTAAAGCTAG TCCCTCCTGG GTTGACCCAG AAGGAGAAAC AGCAGAAAGC
    ACTGAAGATG CACCCTTAGA CTTTCACAGG GTCCTGCACA ATTCTCTGGG AAACATTTCC
    ATGCTGCCAT GTAGCTCCTT CACCCCTAAT TGGCCTGTTC AAAATCCTGA CTCCAGAAAA
    AGTGGTGGCC CAGTCGCAGA GCAGGGCATC GACCCTGATG CCTCCACAGT GGATGAGGAG
    GGGCAACTGC TCGACAGCAT GGATGTTCCC TGCACAAATG GGCACGGCTC TCATAGACTG
    TGCATTCTGA GACAGCCGCC TGGTCAGAGG GCGGAGACCC CCAATTCCTC TGTAAGCGGT
    AACATCCTCT TCCCTGTCCT CAGCGAGGAC TGCACTACCA CAGAGGAAGG AAATCAGCCT
    GGAAACATGC TAAACTGCAG CCAGAACTCC AGCTCATCCT CAGTGTGGTG GCTGAAATCA
    CCTGCATTTT CCAGTGGTTC TTCTGAGGGG GACAGCCCTT GGTCCTATCT GAATTCCAGT
    GGGAGTTCTT GGGTTTCATT GCCGGGAAAG ATGAGGAAAG AGATCCTTGA GGCTCGCACC
    TTGCAACCTG ATGACTTTGA AAAGCTGTTG GCAGGAGTGA GGCATGATTG GCTGTTTCAG
    AGACTAGAGA ATACGGGGGT TTTTAAGCCC AGTCAACTCC ACCGAGCACA TAGTGCTCTT
    TTGTTAAAAT ATTCAAAAAA ATCTGAACTG TGGACGGCCC AGGAAACTAT TGTCTATTTG
    GGGGACTACT TGACTGTGAA GAAAAAAGGC AGACAAAGAA ATGCTTTTTG GGTTCATCAT
    CTTCATCAAG AAGAAATTCT GGGGAGGTAT GTTGGGAAAG ACTATAAGGA GCAGAAGGGG
    CTCTGGCACC ACTTCACTGA TGTGGAGCGA CAGATGACCG CACAGCACTA TGTGACAGAA
    TTTAACAAGA GACTCTATGA ACAAAACATT CCCACCCAGA TATTCTACAT CCCATCCACA
    ATACTACTGA TTTTAGAGGA CAAGACAATA AAGGGATGTA TCAGTGTGGA GCCTTACATA
    CTGGGAGAAT TTGTAAAATT GTCAAATAAC ACGAAAGTGG TGAAAACAGA ATACAAAGCC
    ACAGAATATG GCTTGGCCTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT
    GTGGTCGATT TACAAGGTTG GGTAACCGGT AATGGAAAAG GACTCATCTA CCTCACAGAT
    CCCCAGATTC ACTCCGTTGA TCAGAAAGTT TTCACTACCA ATTTTGGAAA GAGAGGAATT
    TTTTACTTCT TTAATAACCA GCATGTGGAA TGTAATGAAA TCTGCCATCG TCTTTCTTTG
    ACTAGACCTT CAATGGAGAA ACCATGTAAG TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864125.1
    CDS356..4111
    Translation

    Target ORF information:

    RefSeq Version XM_017008636.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008636.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGCCTCC TGTTTTCTCA CAGAGCCTGC TGAAGGAGTT TGACCACCAT
    TTGCTGTCCG CTGCAGAAGC CTGCAAGCTG GCAGCTGCCT TCAGTGCCTA TACGCCGCTC
    TTCGTGCTCA CAGCTGTGAA TATCCGTGGC ACGTGTTTAT TGTCCTACAG TAGTTCAAAT
    GACTGTCCTC CAGAATTGAA AAACTTACAT CTGTGTGAAG CCAAAGAGGC CTTTGAGATT
    GGCCTCCTCA CCAAGAGAGA TGATGAGCCT GTTACTGGAA AACAGGAGCT TCACAGCTTT
    GTCAAAGCTG CTTTCGGTCT CACCACAGTG CACAGAAGGC TCCATGGGGA GACAGGGACG
    GTCCATGCAG CAAGTCAGCT CTGTAAGGAA GCAATGGGGA AGCTGTACAA TTTCAGCACT
    TCCTCCAGAA GTCAGGACAG AGAAGCTCTG TCTCAAGAAG TTATGTCTGT GATTGCCCAG
    GTGAAGGAAC ATTTACAAGT TCAAAGCTTC TCAAATGTAG ATGACAGATC TTATGTTCCC
    GAGAGTTTCG AGTGCAGGTT GGATAAACTT ATCTTGCATG GGCAAGGGGA TTTCCAAAAA
    ATCCTTGACA CCTATTCACA GCACCATACT TCGGTGTGTG AAGTATTTGA AAGTGATTGT
    GGAAACAACA AAAATGAACA GAAAGATGCA AAAACAGGAG TCTGCATCAC TGCTCTAAAA
    ACAGAAATAA AAAACATAGA TACTGTGAGT ACTACTCAAG AAAAGCCACA TTGTCAAAGA
    GACACAGGAA TATCTTCCTC CCTAATGGGT AAGAATGTTC AGAGGGAACT CAGAAGGGGA
    GGAAGGAGAA ACTGGACCCA TTCTGATGCA TTTCGAGTCT CCTTGGATCA AGATGTGGAG
    ACTGAGACTG AGCCATCGGA CTACAGCAAT GGTGAGGGAG CTGTTTTCAA CAAGTCTCTG
    AGTGGCAGCC AGACTTCCAG TGCTTGGAGC AACTTATCAG GGTTTAGTTC CTCTGCAAGC
    TGGGAGGAAG TGAATTATCA CGTTGACGAC AGGTCAGCCA GAAAAGAGCC TGGCAAAGAA
    CATCTGGTGG ACACTCAGTG TTCCACTGCC TTGTCTGAGG AGCTAGAGAA TGACAGGGAA
    GGCAGAGCTA TGCATTCATT GCATTCACAG CTTCATGATC TCTCTCTTCA GGAACCCAAC
    AATGACAATT TGGAGCCTTC TCAAAATCAG CCACAGCAAC AGATGCCCTT GACACCCTTC
    TCGCCTCATA ATACCCCAGG CATTTTCTTG GCCCCTGGTG CAGGGCTTCT AGAAGGAGCT
    CCAGAAGGTA TCCAGGAAGT CAGAAATATG GGACCCAGAA ATACTTCTGC TCACTCCAGA
    CCCTCATATC GTTCTGCTTC TTGGTCTTCT GATTCTGGTA GGCCCAAGAA TATGGGCACA
    CATCCTTCAG TCCAAAAAGA AGAAGCCTTT GAAATAATTG TTGAGTTTCC AGAAACCAAC
    TGCGATGTCA AAGACAGGCA GGGGAAAGAG CAGGGAGAAG AAATTAGTGA AAGAGGCGCA
    GGCCCTACAT TTAAAGCTAG TCCCTCCTGG GTTGACCCAG AAGGAGAAAC AGCAGAAAGC
    ACTGAAGATG CACCCTTAGA CTTTCACAGG GTCCTGCACA ATTCTCTGGG AAACATTTCC
    ATGCTGCCAT GTAGCTCCTT CACCCCTAAT TGGCCTGTTC AAAATCCTGA CTCCAGAAAA
    AGTGGTGGCC CAGTCGCAGA GCAGGGCATC GACCCTGATG CCTCCACAGT GGATGAGGAG
    GGGCAACTGC TCGACAGCAT GGATGTTCCC TGCACAAATG GGCACGGCTC TCATAGACTG
    TGCATTCTGA GACAGCCGCC TGGTCAGAGG GCGGAGACCC CCAATTCCTC TGTAAGCGGT
    AACATCCTCT TCCCTGTCCT CAGCGAGGAC TGCACTACCA CAGAGGAAGG AAATCAGCCT
    GGAAACATGC TAAACTGCAG CCAGAACTCC AGCTCATCCT CAGTGTGGTG GCTGAAATCA
    CCTGCATTTT CCAGTGGTTC TTCTGAGGGG GACAGCCCTT GGTCCTATCT GAATTCCAGT
    GGGAGTTCTT GGGTTTCATT GCCGGGAAAG ATGAGGAAAG AGATCCTTGA GGCTCGCACC
    TTGCAACCTG ATGACTTTGA AAAGCTGTTG GCAGGAGTGA GGCATGATTG GCTGTTTCAG
    AGACTAGAGA ATACGGGGGT TTTTAAGCCC AGTCAACTCC ACCGAGCACA TAGTGCTCTT
    TTGTTAAAAT ATTCAAAAAA ATCTGAACTG TGGACGGCCC AGGAAACTAT TGTCTATTTG
    GGGGACTACT TGACTGTGAA GAAAAAAGGC AGACAAAGAA ATGCTTTTTG GGTTCATCAT
    CTTCATCAAG AAGAAATTCT GGGGAGGTAT GTTGGGAAAG ACTATAAGGA GCAGAAGGGG
    CTCTGGCACC ACTTCACTGA TGTGGAGCGA CAGATGACCG CACAGCACTA TGTGACAGAA
    TTTAACAAGA GACTCTATGA ACAAAACATT CCCACCCAGA TATTCTACAT CCCATCCACA
    ATACTACTGA TTTTAGAGGA CAAGACAATA AAGGGATGTA TCAGTGTGGA GCCTTACATA
    CTGGGAGAAT TTGTAAAATT GTCAAATAAC ACGAAAGTGG TGAAAACAGA ATACAAAGCC
    ACAGAATATG GCTTGGCCTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT
    GTGGTCGATT TACAAGGTTG GGTAACCGGT AATGGAAAAG GACTCATCTA CCTCACAGAT
    CCCCAGATTC ACTCCGTTGA TCAGAAAGTT TTCACTACCA ATTTTGGAAA GAGAGGAATT
    TTTTACTTCT TTAATAACCA GCATGTGGAA TGTAATGAAA TCTGCCATCG TCTTTCTTTG
    ACTAGACCTT CAATGGAGAA ACCATGTAAG TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95411
    Clone ID Related Accession (Same CDS sequence) XM_017008638.2 , XM_017008636.1 , XM_017008635.1 , XM_017008640.1 , XM_017008638.1 , XM_017008633.1 , XM_017008634.1 , XM_017008637.1 , XM_017008639.1 , XM_017008641.2 , XM_017008641.1
    Accession Version XM_017008635.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3756bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGCCTCC TGTTTTCTCA CAGAGCCTGC TGAAGGAGTT TGACCACCAT
    TTGCTGTCCG CTGCAGAAGC CTGCAAGCTG GCAGCTGCCT TCAGTGCCTA TACGCCGCTC
    TTCGTGCTCA CAGCTGTGAA TATCCGTGGC ACGTGTTTAT TGTCCTACAG TAGTTCAAAT
    GACTGTCCTC CAGAATTGAA AAACTTACAT CTGTGTGAAG CCAAAGAGGC CTTTGAGATT
    GGCCTCCTCA CCAAGAGAGA TGATGAGCCT GTTACTGGAA AACAGGAGCT TCACAGCTTT
    GTCAAAGCTG CTTTCGGTCT CACCACAGTG CACAGAAGGC TCCATGGGGA GACAGGGACG
    GTCCATGCAG CAAGTCAGCT CTGTAAGGAA GCAATGGGGA AGCTGTACAA TTTCAGCACT
    TCCTCCAGAA GTCAGGACAG AGAAGCTCTG TCTCAAGAAG TTATGTCTGT GATTGCCCAG
    GTGAAGGAAC ATTTACAAGT TCAAAGCTTC TCAAATGTAG ATGACAGATC TTATGTTCCC
    GAGAGTTTCG AGTGCAGGTT GGATAAACTT ATCTTGCATG GGCAAGGGGA TTTCCAAAAA
    ATCCTTGACA CCTATTCACA GCACCATACT TCGGTGTGTG AAGTATTTGA AAGTGATTGT
    GGAAACAACA AAAATGAACA GAAAGATGCA AAAACAGGAG TCTGCATCAC TGCTCTAAAA
    ACAGAAATAA AAAACATAGA TACTGTGAGT ACTACTCAAG AAAAGCCACA TTGTCAAAGA
    GACACAGGAA TATCTTCCTC CCTAATGGGT AAGAATGTTC AGAGGGAACT CAGAAGGGGA
    GGAAGGAGAA ACTGGACCCA TTCTGATGCA TTTCGAGTCT CCTTGGATCA AGATGTGGAG
    ACTGAGACTG AGCCATCGGA CTACAGCAAT GGTGAGGGAG CTGTTTTCAA CAAGTCTCTG
    AGTGGCAGCC AGACTTCCAG TGCTTGGAGC AACTTATCAG GGTTTAGTTC CTCTGCAAGC
    TGGGAGGAAG TGAATTATCA CGTTGACGAC AGGTCAGCCA GAAAAGAGCC TGGCAAAGAA
    CATCTGGTGG ACACTCAGTG TTCCACTGCC TTGTCTGAGG AGCTAGAGAA TGACAGGGAA
    GGCAGAGCTA TGCATTCATT GCATTCACAG CTTCATGATC TCTCTCTTCA GGAACCCAAC
    AATGACAATT TGGAGCCTTC TCAAAATCAG CCACAGCAAC AGATGCCCTT GACACCCTTC
    TCGCCTCATA ATACCCCAGG CATTTTCTTG GCCCCTGGTG CAGGGCTTCT AGAAGGAGCT
    CCAGAAGGTA TCCAGGAAGT CAGAAATATG GGACCCAGAA ATACTTCTGC TCACTCCAGA
    CCCTCATATC GTTCTGCTTC TTGGTCTTCT GATTCTGGTA GGCCCAAGAA TATGGGCACA
    CATCCTTCAG TCCAAAAAGA AGAAGCCTTT GAAATAATTG TTGAGTTTCC AGAAACCAAC
    TGCGATGTCA AAGACAGGCA GGGGAAAGAG CAGGGAGAAG AAATTAGTGA AAGAGGCGCA
    GGCCCTACAT TTAAAGCTAG TCCCTCCTGG GTTGACCCAG AAGGAGAAAC AGCAGAAAGC
    ACTGAAGATG CACCCTTAGA CTTTCACAGG GTCCTGCACA ATTCTCTGGG AAACATTTCC
    ATGCTGCCAT GTAGCTCCTT CACCCCTAAT TGGCCTGTTC AAAATCCTGA CTCCAGAAAA
    AGTGGTGGCC CAGTCGCAGA GCAGGGCATC GACCCTGATG CCTCCACAGT GGATGAGGAG
    GGGCAACTGC TCGACAGCAT GGATGTTCCC TGCACAAATG GGCACGGCTC TCATAGACTG
    TGCATTCTGA GACAGCCGCC TGGTCAGAGG GCGGAGACCC CCAATTCCTC TGTAAGCGGT
    AACATCCTCT TCCCTGTCCT CAGCGAGGAC TGCACTACCA CAGAGGAAGG AAATCAGCCT
    GGAAACATGC TAAACTGCAG CCAGAACTCC AGCTCATCCT CAGTGTGGTG GCTGAAATCA
    CCTGCATTTT CCAGTGGTTC TTCTGAGGGG GACAGCCCTT GGTCCTATCT GAATTCCAGT
    GGGAGTTCTT GGGTTTCATT GCCGGGAAAG ATGAGGAAAG AGATCCTTGA GGCTCGCACC
    TTGCAACCTG ATGACTTTGA AAAGCTGTTG GCAGGAGTGA GGCATGATTG GCTGTTTCAG
    AGACTAGAGA ATACGGGGGT TTTTAAGCCC AGTCAACTCC ACCGAGCACA TAGTGCTCTT
    TTGTTAAAAT ATTCAAAAAA ATCTGAACTG TGGACGGCCC AGGAAACTAT TGTCTATTTG
    GGGGACTACT TGACTGTGAA GAAAAAAGGC AGACAAAGAA ATGCTTTTTG GGTTCATCAT
    CTTCATCAAG AAGAAATTCT GGGGAGGTAT GTTGGGAAAG ACTATAAGGA GCAGAAGGGG
    CTCTGGCACC ACTTCACTGA TGTGGAGCGA CAGATGACCG CACAGCACTA TGTGACAGAA
    TTTAACAAGA GACTCTATGA ACAAAACATT CCCACCCAGA TATTCTACAT CCCATCCACA
    ATACTACTGA TTTTAGAGGA CAAGACAATA AAGGGATGTA TCAGTGTGGA GCCTTACATA
    CTGGGAGAAT TTGTAAAATT GTCAAATAAC ACGAAAGTGG TGAAAACAGA ATACAAAGCC
    ACAGAATATG GCTTGGCCTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT
    GTGGTCGATT TACAAGGTTG GGTAACCGGT AATGGAAAAG GACTCATCTA CCTCACAGAT
    CCCCAGATTC ACTCCGTTGA TCAGAAAGTT TTCACTACCA ATTTTGGAAA GAGAGGAATT
    TTTTACTTCT TTAATAACCA GCATGTGGAA TGTAATGAAA TCTGCCATCG TCTTTCTTTG
    ACTAGACCTT CAATGGAGAA ACCATGTAAG TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864124.1
    CDS203..3958
    Translation

    Target ORF information:

    RefSeq Version XM_017008635.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008635.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGCCTCC TGTTTTCTCA CAGAGCCTGC TGAAGGAGTT TGACCACCAT
    TTGCTGTCCG CTGCAGAAGC CTGCAAGCTG GCAGCTGCCT TCAGTGCCTA TACGCCGCTC
    TTCGTGCTCA CAGCTGTGAA TATCCGTGGC ACGTGTTTAT TGTCCTACAG TAGTTCAAAT
    GACTGTCCTC CAGAATTGAA AAACTTACAT CTGTGTGAAG CCAAAGAGGC CTTTGAGATT
    GGCCTCCTCA CCAAGAGAGA TGATGAGCCT GTTACTGGAA AACAGGAGCT TCACAGCTTT
    GTCAAAGCTG CTTTCGGTCT CACCACAGTG CACAGAAGGC TCCATGGGGA GACAGGGACG
    GTCCATGCAG CAAGTCAGCT CTGTAAGGAA GCAATGGGGA AGCTGTACAA TTTCAGCACT
    TCCTCCAGAA GTCAGGACAG AGAAGCTCTG TCTCAAGAAG TTATGTCTGT GATTGCCCAG
    GTGAAGGAAC ATTTACAAGT TCAAAGCTTC TCAAATGTAG ATGACAGATC TTATGTTCCC
    GAGAGTTTCG AGTGCAGGTT GGATAAACTT ATCTTGCATG GGCAAGGGGA TTTCCAAAAA
    ATCCTTGACA CCTATTCACA GCACCATACT TCGGTGTGTG AAGTATTTGA AAGTGATTGT
    GGAAACAACA AAAATGAACA GAAAGATGCA AAAACAGGAG TCTGCATCAC TGCTCTAAAA
    ACAGAAATAA AAAACATAGA TACTGTGAGT ACTACTCAAG AAAAGCCACA TTGTCAAAGA
    GACACAGGAA TATCTTCCTC CCTAATGGGT AAGAATGTTC AGAGGGAACT CAGAAGGGGA
    GGAAGGAGAA ACTGGACCCA TTCTGATGCA TTTCGAGTCT CCTTGGATCA AGATGTGGAG
    ACTGAGACTG AGCCATCGGA CTACAGCAAT GGTGAGGGAG CTGTTTTCAA CAAGTCTCTG
    AGTGGCAGCC AGACTTCCAG TGCTTGGAGC AACTTATCAG GGTTTAGTTC CTCTGCAAGC
    TGGGAGGAAG TGAATTATCA CGTTGACGAC AGGTCAGCCA GAAAAGAGCC TGGCAAAGAA
    CATCTGGTGG ACACTCAGTG TTCCACTGCC TTGTCTGAGG AGCTAGAGAA TGACAGGGAA
    GGCAGAGCTA TGCATTCATT GCATTCACAG CTTCATGATC TCTCTCTTCA GGAACCCAAC
    AATGACAATT TGGAGCCTTC TCAAAATCAG CCACAGCAAC AGATGCCCTT GACACCCTTC
    TCGCCTCATA ATACCCCAGG CATTTTCTTG GCCCCTGGTG CAGGGCTTCT AGAAGGAGCT
    CCAGAAGGTA TCCAGGAAGT CAGAAATATG GGACCCAGAA ATACTTCTGC TCACTCCAGA
    CCCTCATATC GTTCTGCTTC TTGGTCTTCT GATTCTGGTA GGCCCAAGAA TATGGGCACA
    CATCCTTCAG TCCAAAAAGA AGAAGCCTTT GAAATAATTG TTGAGTTTCC AGAAACCAAC
    TGCGATGTCA AAGACAGGCA GGGGAAAGAG CAGGGAGAAG AAATTAGTGA AAGAGGCGCA
    GGCCCTACAT TTAAAGCTAG TCCCTCCTGG GTTGACCCAG AAGGAGAAAC AGCAGAAAGC
    ACTGAAGATG CACCCTTAGA CTTTCACAGG GTCCTGCACA ATTCTCTGGG AAACATTTCC
    ATGCTGCCAT GTAGCTCCTT CACCCCTAAT TGGCCTGTTC AAAATCCTGA CTCCAGAAAA
    AGTGGTGGCC CAGTCGCAGA GCAGGGCATC GACCCTGATG CCTCCACAGT GGATGAGGAG
    GGGCAACTGC TCGACAGCAT GGATGTTCCC TGCACAAATG GGCACGGCTC TCATAGACTG
    TGCATTCTGA GACAGCCGCC TGGTCAGAGG GCGGAGACCC CCAATTCCTC TGTAAGCGGT
    AACATCCTCT TCCCTGTCCT CAGCGAGGAC TGCACTACCA CAGAGGAAGG AAATCAGCCT
    GGAAACATGC TAAACTGCAG CCAGAACTCC AGCTCATCCT CAGTGTGGTG GCTGAAATCA
    CCTGCATTTT CCAGTGGTTC TTCTGAGGGG GACAGCCCTT GGTCCTATCT GAATTCCAGT
    GGGAGTTCTT GGGTTTCATT GCCGGGAAAG ATGAGGAAAG AGATCCTTGA GGCTCGCACC
    TTGCAACCTG ATGACTTTGA AAAGCTGTTG GCAGGAGTGA GGCATGATTG GCTGTTTCAG
    AGACTAGAGA ATACGGGGGT TTTTAAGCCC AGTCAACTCC ACCGAGCACA TAGTGCTCTT
    TTGTTAAAAT ATTCAAAAAA ATCTGAACTG TGGACGGCCC AGGAAACTAT TGTCTATTTG
    GGGGACTACT TGACTGTGAA GAAAAAAGGC AGACAAAGAA ATGCTTTTTG GGTTCATCAT
    CTTCATCAAG AAGAAATTCT GGGGAGGTAT GTTGGGAAAG ACTATAAGGA GCAGAAGGGG
    CTCTGGCACC ACTTCACTGA TGTGGAGCGA CAGATGACCG CACAGCACTA TGTGACAGAA
    TTTAACAAGA GACTCTATGA ACAAAACATT CCCACCCAGA TATTCTACAT CCCATCCACA
    ATACTACTGA TTTTAGAGGA CAAGACAATA AAGGGATGTA TCAGTGTGGA GCCTTACATA
    CTGGGAGAAT TTGTAAAATT GTCAAATAAC ACGAAAGTGG TGAAAACAGA ATACAAAGCC
    ACAGAATATG GCTTGGCCTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT
    GTGGTCGATT TACAAGGTTG GGTAACCGGT AATGGAAAAG GACTCATCTA CCTCACAGAT
    CCCCAGATTC ACTCCGTTGA TCAGAAAGTT TTCACTACCA ATTTTGGAAA GAGAGGAATT
    TTTTACTTCT TTAATAACCA GCATGTGGAA TGTAATGAAA TCTGCCATCG TCTTTCTTTG
    ACTAGACCTT CAATGGAGAA ACCATGTAAG TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95411
    Clone ID Related Accession (Same CDS sequence) XM_017008638.2 , XM_017008636.1 , XM_017008635.1 , XM_017008640.1 , XM_017008638.1 , XM_017008633.1 , XM_017008634.1 , XM_017008637.1 , XM_017008639.1 , XM_017008641.2 , XM_017008641.1
    Accession Version XM_017008640.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3756bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGCCTCC TGTTTTCTCA CAGAGCCTGC TGAAGGAGTT TGACCACCAT
    TTGCTGTCCG CTGCAGAAGC CTGCAAGCTG GCAGCTGCCT TCAGTGCCTA TACGCCGCTC
    TTCGTGCTCA CAGCTGTGAA TATCCGTGGC ACGTGTTTAT TGTCCTACAG TAGTTCAAAT
    GACTGTCCTC CAGAATTGAA AAACTTACAT CTGTGTGAAG CCAAAGAGGC CTTTGAGATT
    GGCCTCCTCA CCAAGAGAGA TGATGAGCCT GTTACTGGAA AACAGGAGCT TCACAGCTTT
    GTCAAAGCTG CTTTCGGTCT CACCACAGTG CACAGAAGGC TCCATGGGGA GACAGGGACG
    GTCCATGCAG CAAGTCAGCT CTGTAAGGAA GCAATGGGGA AGCTGTACAA TTTCAGCACT
    TCCTCCAGAA GTCAGGACAG AGAAGCTCTG TCTCAAGAAG TTATGTCTGT GATTGCCCAG
    GTGAAGGAAC ATTTACAAGT TCAAAGCTTC TCAAATGTAG ATGACAGATC TTATGTTCCC
    GAGAGTTTCG AGTGCAGGTT GGATAAACTT ATCTTGCATG GGCAAGGGGA TTTCCAAAAA
    ATCCTTGACA CCTATTCACA GCACCATACT TCGGTGTGTG AAGTATTTGA AAGTGATTGT
    GGAAACAACA AAAATGAACA GAAAGATGCA AAAACAGGAG TCTGCATCAC TGCTCTAAAA
    ACAGAAATAA AAAACATAGA TACTGTGAGT ACTACTCAAG AAAAGCCACA TTGTCAAAGA
    GACACAGGAA TATCTTCCTC CCTAATGGGT AAGAATGTTC AGAGGGAACT CAGAAGGGGA
    GGAAGGAGAA ACTGGACCCA TTCTGATGCA TTTCGAGTCT CCTTGGATCA AGATGTGGAG
    ACTGAGACTG AGCCATCGGA CTACAGCAAT GGTGAGGGAG CTGTTTTCAA CAAGTCTCTG
    AGTGGCAGCC AGACTTCCAG TGCTTGGAGC AACTTATCAG GGTTTAGTTC CTCTGCAAGC
    TGGGAGGAAG TGAATTATCA CGTTGACGAC AGGTCAGCCA GAAAAGAGCC TGGCAAAGAA
    CATCTGGTGG ACACTCAGTG TTCCACTGCC TTGTCTGAGG AGCTAGAGAA TGACAGGGAA
    GGCAGAGCTA TGCATTCATT GCATTCACAG CTTCATGATC TCTCTCTTCA GGAACCCAAC
    AATGACAATT TGGAGCCTTC TCAAAATCAG CCACAGCAAC AGATGCCCTT GACACCCTTC
    TCGCCTCATA ATACCCCAGG CATTTTCTTG GCCCCTGGTG CAGGGCTTCT AGAAGGAGCT
    CCAGAAGGTA TCCAGGAAGT CAGAAATATG GGACCCAGAA ATACTTCTGC TCACTCCAGA
    CCCTCATATC GTTCTGCTTC TTGGTCTTCT GATTCTGGTA GGCCCAAGAA TATGGGCACA
    CATCCTTCAG TCCAAAAAGA AGAAGCCTTT GAAATAATTG TTGAGTTTCC AGAAACCAAC
    TGCGATGTCA AAGACAGGCA GGGGAAAGAG CAGGGAGAAG AAATTAGTGA AAGAGGCGCA
    GGCCCTACAT TTAAAGCTAG TCCCTCCTGG GTTGACCCAG AAGGAGAAAC AGCAGAAAGC
    ACTGAAGATG CACCCTTAGA CTTTCACAGG GTCCTGCACA ATTCTCTGGG AAACATTTCC
    ATGCTGCCAT GTAGCTCCTT CACCCCTAAT TGGCCTGTTC AAAATCCTGA CTCCAGAAAA
    AGTGGTGGCC CAGTCGCAGA GCAGGGCATC GACCCTGATG CCTCCACAGT GGATGAGGAG
    GGGCAACTGC TCGACAGCAT GGATGTTCCC TGCACAAATG GGCACGGCTC TCATAGACTG
    TGCATTCTGA GACAGCCGCC TGGTCAGAGG GCGGAGACCC CCAATTCCTC TGTAAGCGGT
    AACATCCTCT TCCCTGTCCT CAGCGAGGAC TGCACTACCA CAGAGGAAGG AAATCAGCCT
    GGAAACATGC TAAACTGCAG CCAGAACTCC AGCTCATCCT CAGTGTGGTG GCTGAAATCA
    CCTGCATTTT CCAGTGGTTC TTCTGAGGGG GACAGCCCTT GGTCCTATCT GAATTCCAGT
    GGGAGTTCTT GGGTTTCATT GCCGGGAAAG ATGAGGAAAG AGATCCTTGA GGCTCGCACC
    TTGCAACCTG ATGACTTTGA AAAGCTGTTG GCAGGAGTGA GGCATGATTG GCTGTTTCAG
    AGACTAGAGA ATACGGGGGT TTTTAAGCCC AGTCAACTCC ACCGAGCACA TAGTGCTCTT
    TTGTTAAAAT ATTCAAAAAA ATCTGAACTG TGGACGGCCC AGGAAACTAT TGTCTATTTG
    GGGGACTACT TGACTGTGAA GAAAAAAGGC AGACAAAGAA ATGCTTTTTG GGTTCATCAT
    CTTCATCAAG AAGAAATTCT GGGGAGGTAT GTTGGGAAAG ACTATAAGGA GCAGAAGGGG
    CTCTGGCACC ACTTCACTGA TGTGGAGCGA CAGATGACCG CACAGCACTA TGTGACAGAA
    TTTAACAAGA GACTCTATGA ACAAAACATT CCCACCCAGA TATTCTACAT CCCATCCACA
    ATACTACTGA TTTTAGAGGA CAAGACAATA AAGGGATGTA TCAGTGTGGA GCCTTACATA
    CTGGGAGAAT TTGTAAAATT GTCAAATAAC ACGAAAGTGG TGAAAACAGA ATACAAAGCC
    ACAGAATATG GCTTGGCCTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT
    GTGGTCGATT TACAAGGTTG GGTAACCGGT AATGGAAAAG GACTCATCTA CCTCACAGAT
    CCCCAGATTC ACTCCGTTGA TCAGAAAGTT TTCACTACCA ATTTTGGAAA GAGAGGAATT
    TTTTACTTCT TTAATAACCA GCATGTGGAA TGTAATGAAA TCTGCCATCG TCTTTCTTTG
    ACTAGACCTT CAATGGAGAA ACCATGTAAG TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864129.1
    CDS179..3934
    Translation

    Target ORF information:

    RefSeq Version XM_017008640.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008640.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGCCTCC TGTTTTCTCA CAGAGCCTGC TGAAGGAGTT TGACCACCAT
    TTGCTGTCCG CTGCAGAAGC CTGCAAGCTG GCAGCTGCCT TCAGTGCCTA TACGCCGCTC
    TTCGTGCTCA CAGCTGTGAA TATCCGTGGC ACGTGTTTAT TGTCCTACAG TAGTTCAAAT
    GACTGTCCTC CAGAATTGAA AAACTTACAT CTGTGTGAAG CCAAAGAGGC CTTTGAGATT
    GGCCTCCTCA CCAAGAGAGA TGATGAGCCT GTTACTGGAA AACAGGAGCT TCACAGCTTT
    GTCAAAGCTG CTTTCGGTCT CACCACAGTG CACAGAAGGC TCCATGGGGA GACAGGGACG
    GTCCATGCAG CAAGTCAGCT CTGTAAGGAA GCAATGGGGA AGCTGTACAA TTTCAGCACT
    TCCTCCAGAA GTCAGGACAG AGAAGCTCTG TCTCAAGAAG TTATGTCTGT GATTGCCCAG
    GTGAAGGAAC ATTTACAAGT TCAAAGCTTC TCAAATGTAG ATGACAGATC TTATGTTCCC
    GAGAGTTTCG AGTGCAGGTT GGATAAACTT ATCTTGCATG GGCAAGGGGA TTTCCAAAAA
    ATCCTTGACA CCTATTCACA GCACCATACT TCGGTGTGTG AAGTATTTGA AAGTGATTGT
    GGAAACAACA AAAATGAACA GAAAGATGCA AAAACAGGAG TCTGCATCAC TGCTCTAAAA
    ACAGAAATAA AAAACATAGA TACTGTGAGT ACTACTCAAG AAAAGCCACA TTGTCAAAGA
    GACACAGGAA TATCTTCCTC CCTAATGGGT AAGAATGTTC AGAGGGAACT CAGAAGGGGA
    GGAAGGAGAA ACTGGACCCA TTCTGATGCA TTTCGAGTCT CCTTGGATCA AGATGTGGAG
    ACTGAGACTG AGCCATCGGA CTACAGCAAT GGTGAGGGAG CTGTTTTCAA CAAGTCTCTG
    AGTGGCAGCC AGACTTCCAG TGCTTGGAGC AACTTATCAG GGTTTAGTTC CTCTGCAAGC
    TGGGAGGAAG TGAATTATCA CGTTGACGAC AGGTCAGCCA GAAAAGAGCC TGGCAAAGAA
    CATCTGGTGG ACACTCAGTG TTCCACTGCC TTGTCTGAGG AGCTAGAGAA TGACAGGGAA
    GGCAGAGCTA TGCATTCATT GCATTCACAG CTTCATGATC TCTCTCTTCA GGAACCCAAC
    AATGACAATT TGGAGCCTTC TCAAAATCAG CCACAGCAAC AGATGCCCTT GACACCCTTC
    TCGCCTCATA ATACCCCAGG CATTTTCTTG GCCCCTGGTG CAGGGCTTCT AGAAGGAGCT
    CCAGAAGGTA TCCAGGAAGT CAGAAATATG GGACCCAGAA ATACTTCTGC TCACTCCAGA
    CCCTCATATC GTTCTGCTTC TTGGTCTTCT GATTCTGGTA GGCCCAAGAA TATGGGCACA
    CATCCTTCAG TCCAAAAAGA AGAAGCCTTT GAAATAATTG TTGAGTTTCC AGAAACCAAC
    TGCGATGTCA AAGACAGGCA GGGGAAAGAG CAGGGAGAAG AAATTAGTGA AAGAGGCGCA
    GGCCCTACAT TTAAAGCTAG TCCCTCCTGG GTTGACCCAG AAGGAGAAAC AGCAGAAAGC
    ACTGAAGATG CACCCTTAGA CTTTCACAGG GTCCTGCACA ATTCTCTGGG AAACATTTCC
    ATGCTGCCAT GTAGCTCCTT CACCCCTAAT TGGCCTGTTC AAAATCCTGA CTCCAGAAAA
    AGTGGTGGCC CAGTCGCAGA GCAGGGCATC GACCCTGATG CCTCCACAGT GGATGAGGAG
    GGGCAACTGC TCGACAGCAT GGATGTTCCC TGCACAAATG GGCACGGCTC TCATAGACTG
    TGCATTCTGA GACAGCCGCC TGGTCAGAGG GCGGAGACCC CCAATTCCTC TGTAAGCGGT
    AACATCCTCT TCCCTGTCCT CAGCGAGGAC TGCACTACCA CAGAGGAAGG AAATCAGCCT
    GGAAACATGC TAAACTGCAG CCAGAACTCC AGCTCATCCT CAGTGTGGTG GCTGAAATCA
    CCTGCATTTT CCAGTGGTTC TTCTGAGGGG GACAGCCCTT GGTCCTATCT GAATTCCAGT
    GGGAGTTCTT GGGTTTCATT GCCGGGAAAG ATGAGGAAAG AGATCCTTGA GGCTCGCACC
    TTGCAACCTG ATGACTTTGA AAAGCTGTTG GCAGGAGTGA GGCATGATTG GCTGTTTCAG
    AGACTAGAGA ATACGGGGGT TTTTAAGCCC AGTCAACTCC ACCGAGCACA TAGTGCTCTT
    TTGTTAAAAT ATTCAAAAAA ATCTGAACTG TGGACGGCCC AGGAAACTAT TGTCTATTTG
    GGGGACTACT TGACTGTGAA GAAAAAAGGC AGACAAAGAA ATGCTTTTTG GGTTCATCAT
    CTTCATCAAG AAGAAATTCT GGGGAGGTAT GTTGGGAAAG ACTATAAGGA GCAGAAGGGG
    CTCTGGCACC ACTTCACTGA TGTGGAGCGA CAGATGACCG CACAGCACTA TGTGACAGAA
    TTTAACAAGA GACTCTATGA ACAAAACATT CCCACCCAGA TATTCTACAT CCCATCCACA
    ATACTACTGA TTTTAGAGGA CAAGACAATA AAGGGATGTA TCAGTGTGGA GCCTTACATA
    CTGGGAGAAT TTGTAAAATT GTCAAATAAC ACGAAAGTGG TGAAAACAGA ATACAAAGCC
    ACAGAATATG GCTTGGCCTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT
    GTGGTCGATT TACAAGGTTG GGTAACCGGT AATGGAAAAG GACTCATCTA CCTCACAGAT
    CCCCAGATTC ACTCCGTTGA TCAGAAAGTT TTCACTACCA ATTTTGGAAA GAGAGGAATT
    TTTTACTTCT TTAATAACCA GCATGTGGAA TGTAATGAAA TCTGCCATCG TCTTTCTTTG
    ACTAGACCTT CAATGGAGAA ACCATGTAAG TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95411
    Clone ID Related Accession (Same CDS sequence) XM_017008638.2 , XM_017008636.1 , XM_017008635.1 , XM_017008640.1 , XM_017008638.1 , XM_017008633.1 , XM_017008634.1 , XM_017008637.1 , XM_017008639.1 , XM_017008641.2 , XM_017008641.1
    Accession Version XM_017008638.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3756bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGCCTCC TGTTTTCTCA CAGAGCCTGC TGAAGGAGTT TGACCACCAT
    TTGCTGTCCG CTGCAGAAGC CTGCAAGCTG GCAGCTGCCT TCAGTGCCTA TACGCCGCTC
    TTCGTGCTCA CAGCTGTGAA TATCCGTGGC ACGTGTTTAT TGTCCTACAG TAGTTCAAAT
    GACTGTCCTC CAGAATTGAA AAACTTACAT CTGTGTGAAG CCAAAGAGGC CTTTGAGATT
    GGCCTCCTCA CCAAGAGAGA TGATGAGCCT GTTACTGGAA AACAGGAGCT TCACAGCTTT
    GTCAAAGCTG CTTTCGGTCT CACCACAGTG CACAGAAGGC TCCATGGGGA GACAGGGACG
    GTCCATGCAG CAAGTCAGCT CTGTAAGGAA GCAATGGGGA AGCTGTACAA TTTCAGCACT
    TCCTCCAGAA GTCAGGACAG AGAAGCTCTG TCTCAAGAAG TTATGTCTGT GATTGCCCAG
    GTGAAGGAAC ATTTACAAGT TCAAAGCTTC TCAAATGTAG ATGACAGATC TTATGTTCCC
    GAGAGTTTCG AGTGCAGGTT GGATAAACTT ATCTTGCATG GGCAAGGGGA TTTCCAAAAA
    ATCCTTGACA CCTATTCACA GCACCATACT TCGGTGTGTG AAGTATTTGA AAGTGATTGT
    GGAAACAACA AAAATGAACA GAAAGATGCA AAAACAGGAG TCTGCATCAC TGCTCTAAAA
    ACAGAAATAA AAAACATAGA TACTGTGAGT ACTACTCAAG AAAAGCCACA TTGTCAAAGA
    GACACAGGAA TATCTTCCTC CCTAATGGGT AAGAATGTTC AGAGGGAACT CAGAAGGGGA
    GGAAGGAGAA ACTGGACCCA TTCTGATGCA TTTCGAGTCT CCTTGGATCA AGATGTGGAG
    ACTGAGACTG AGCCATCGGA CTACAGCAAT GGTGAGGGAG CTGTTTTCAA CAAGTCTCTG
    AGTGGCAGCC AGACTTCCAG TGCTTGGAGC AACTTATCAG GGTTTAGTTC CTCTGCAAGC
    TGGGAGGAAG TGAATTATCA CGTTGACGAC AGGTCAGCCA GAAAAGAGCC TGGCAAAGAA
    CATCTGGTGG ACACTCAGTG TTCCACTGCC TTGTCTGAGG AGCTAGAGAA TGACAGGGAA
    GGCAGAGCTA TGCATTCATT GCATTCACAG CTTCATGATC TCTCTCTTCA GGAACCCAAC
    AATGACAATT TGGAGCCTTC TCAAAATCAG CCACAGCAAC AGATGCCCTT GACACCCTTC
    TCGCCTCATA ATACCCCAGG CATTTTCTTG GCCCCTGGTG CAGGGCTTCT AGAAGGAGCT
    CCAGAAGGTA TCCAGGAAGT CAGAAATATG GGACCCAGAA ATACTTCTGC TCACTCCAGA
    CCCTCATATC GTTCTGCTTC TTGGTCTTCT GATTCTGGTA GGCCCAAGAA TATGGGCACA
    CATCCTTCAG TCCAAAAAGA AGAAGCCTTT GAAATAATTG TTGAGTTTCC AGAAACCAAC
    TGCGATGTCA AAGACAGGCA GGGGAAAGAG CAGGGAGAAG AAATTAGTGA AAGAGGCGCA
    GGCCCTACAT TTAAAGCTAG TCCCTCCTGG GTTGACCCAG AAGGAGAAAC AGCAGAAAGC
    ACTGAAGATG CACCCTTAGA CTTTCACAGG GTCCTGCACA ATTCTCTGGG AAACATTTCC
    ATGCTGCCAT GTAGCTCCTT CACCCCTAAT TGGCCTGTTC AAAATCCTGA CTCCAGAAAA
    AGTGGTGGCC CAGTCGCAGA GCAGGGCATC GACCCTGATG CCTCCACAGT GGATGAGGAG
    GGGCAACTGC TCGACAGCAT GGATGTTCCC TGCACAAATG GGCACGGCTC TCATAGACTG
    TGCATTCTGA GACAGCCGCC TGGTCAGAGG GCGGAGACCC CCAATTCCTC TGTAAGCGGT
    AACATCCTCT TCCCTGTCCT CAGCGAGGAC TGCACTACCA CAGAGGAAGG AAATCAGCCT
    GGAAACATGC TAAACTGCAG CCAGAACTCC AGCTCATCCT CAGTGTGGTG GCTGAAATCA
    CCTGCATTTT CCAGTGGTTC TTCTGAGGGG GACAGCCCTT GGTCCTATCT GAATTCCAGT
    GGGAGTTCTT GGGTTTCATT GCCGGGAAAG ATGAGGAAAG AGATCCTTGA GGCTCGCACC
    TTGCAACCTG ATGACTTTGA AAAGCTGTTG GCAGGAGTGA GGCATGATTG GCTGTTTCAG
    AGACTAGAGA ATACGGGGGT TTTTAAGCCC AGTCAACTCC ACCGAGCACA TAGTGCTCTT
    TTGTTAAAAT ATTCAAAAAA ATCTGAACTG TGGACGGCCC AGGAAACTAT TGTCTATTTG
    GGGGACTACT TGACTGTGAA GAAAAAAGGC AGACAAAGAA ATGCTTTTTG GGTTCATCAT
    CTTCATCAAG AAGAAATTCT GGGGAGGTAT GTTGGGAAAG ACTATAAGGA GCAGAAGGGG
    CTCTGGCACC ACTTCACTGA TGTGGAGCGA CAGATGACCG CACAGCACTA TGTGACAGAA
    TTTAACAAGA GACTCTATGA ACAAAACATT CCCACCCAGA TATTCTACAT CCCATCCACA
    ATACTACTGA TTTTAGAGGA CAAGACAATA AAGGGATGTA TCAGTGTGGA GCCTTACATA
    CTGGGAGAAT TTGTAAAATT GTCAAATAAC ACGAAAGTGG TGAAAACAGA ATACAAAGCC
    ACAGAATATG GCTTGGCCTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT
    GTGGTCGATT TACAAGGTTG GGTAACCGGT AATGGAAAAG GACTCATCTA CCTCACAGAT
    CCCCAGATTC ACTCCGTTGA TCAGAAAGTT TTCACTACCA ATTTTGGAAA GAGAGGAATT
    TTTTACTTCT TTAATAACCA GCATGTGGAA TGTAATGAAA TCTGCCATCG TCTTTCTTTG
    ACTAGACCTT CAATGGAGAA ACCATGTAAG TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864127.1
    CDS369..4124
    Translation

    Target ORF information:

    RefSeq Version XM_017008638.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008638.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGCCTCC TGTTTTCTCA CAGAGCCTGC TGAAGGAGTT TGACCACCAT
    TTGCTGTCCG CTGCAGAAGC CTGCAAGCTG GCAGCTGCCT TCAGTGCCTA TACGCCGCTC
    TTCGTGCTCA CAGCTGTGAA TATCCGTGGC ACGTGTTTAT TGTCCTACAG TAGTTCAAAT
    GACTGTCCTC CAGAATTGAA AAACTTACAT CTGTGTGAAG CCAAAGAGGC CTTTGAGATT
    GGCCTCCTCA CCAAGAGAGA TGATGAGCCT GTTACTGGAA AACAGGAGCT TCACAGCTTT
    GTCAAAGCTG CTTTCGGTCT CACCACAGTG CACAGAAGGC TCCATGGGGA GACAGGGACG
    GTCCATGCAG CAAGTCAGCT CTGTAAGGAA GCAATGGGGA AGCTGTACAA TTTCAGCACT
    TCCTCCAGAA GTCAGGACAG AGAAGCTCTG TCTCAAGAAG TTATGTCTGT GATTGCCCAG
    GTGAAGGAAC ATTTACAAGT TCAAAGCTTC TCAAATGTAG ATGACAGATC TTATGTTCCC
    GAGAGTTTCG AGTGCAGGTT GGATAAACTT ATCTTGCATG GGCAAGGGGA TTTCCAAAAA
    ATCCTTGACA CCTATTCACA GCACCATACT TCGGTGTGTG AAGTATTTGA AAGTGATTGT
    GGAAACAACA AAAATGAACA GAAAGATGCA AAAACAGGAG TCTGCATCAC TGCTCTAAAA
    ACAGAAATAA AAAACATAGA TACTGTGAGT ACTACTCAAG AAAAGCCACA TTGTCAAAGA
    GACACAGGAA TATCTTCCTC CCTAATGGGT AAGAATGTTC AGAGGGAACT CAGAAGGGGA
    GGAAGGAGAA ACTGGACCCA TTCTGATGCA TTTCGAGTCT CCTTGGATCA AGATGTGGAG
    ACTGAGACTG AGCCATCGGA CTACAGCAAT GGTGAGGGAG CTGTTTTCAA CAAGTCTCTG
    AGTGGCAGCC AGACTTCCAG TGCTTGGAGC AACTTATCAG GGTTTAGTTC CTCTGCAAGC
    TGGGAGGAAG TGAATTATCA CGTTGACGAC AGGTCAGCCA GAAAAGAGCC TGGCAAAGAA
    CATCTGGTGG ACACTCAGTG TTCCACTGCC TTGTCTGAGG AGCTAGAGAA TGACAGGGAA
    GGCAGAGCTA TGCATTCATT GCATTCACAG CTTCATGATC TCTCTCTTCA GGAACCCAAC
    AATGACAATT TGGAGCCTTC TCAAAATCAG CCACAGCAAC AGATGCCCTT GACACCCTTC
    TCGCCTCATA ATACCCCAGG CATTTTCTTG GCCCCTGGTG CAGGGCTTCT AGAAGGAGCT
    CCAGAAGGTA TCCAGGAAGT CAGAAATATG GGACCCAGAA ATACTTCTGC TCACTCCAGA
    CCCTCATATC GTTCTGCTTC TTGGTCTTCT GATTCTGGTA GGCCCAAGAA TATGGGCACA
    CATCCTTCAG TCCAAAAAGA AGAAGCCTTT GAAATAATTG TTGAGTTTCC AGAAACCAAC
    TGCGATGTCA AAGACAGGCA GGGGAAAGAG CAGGGAGAAG AAATTAGTGA AAGAGGCGCA
    GGCCCTACAT TTAAAGCTAG TCCCTCCTGG GTTGACCCAG AAGGAGAAAC AGCAGAAAGC
    ACTGAAGATG CACCCTTAGA CTTTCACAGG GTCCTGCACA ATTCTCTGGG AAACATTTCC
    ATGCTGCCAT GTAGCTCCTT CACCCCTAAT TGGCCTGTTC AAAATCCTGA CTCCAGAAAA
    AGTGGTGGCC CAGTCGCAGA GCAGGGCATC GACCCTGATG CCTCCACAGT GGATGAGGAG
    GGGCAACTGC TCGACAGCAT GGATGTTCCC TGCACAAATG GGCACGGCTC TCATAGACTG
    TGCATTCTGA GACAGCCGCC TGGTCAGAGG GCGGAGACCC CCAATTCCTC TGTAAGCGGT
    AACATCCTCT TCCCTGTCCT CAGCGAGGAC TGCACTACCA CAGAGGAAGG AAATCAGCCT
    GGAAACATGC TAAACTGCAG CCAGAACTCC AGCTCATCCT CAGTGTGGTG GCTGAAATCA
    CCTGCATTTT CCAGTGGTTC TTCTGAGGGG GACAGCCCTT GGTCCTATCT GAATTCCAGT
    GGGAGTTCTT GGGTTTCATT GCCGGGAAAG ATGAGGAAAG AGATCCTTGA GGCTCGCACC
    TTGCAACCTG ATGACTTTGA AAAGCTGTTG GCAGGAGTGA GGCATGATTG GCTGTTTCAG
    AGACTAGAGA ATACGGGGGT TTTTAAGCCC AGTCAACTCC ACCGAGCACA TAGTGCTCTT
    TTGTTAAAAT ATTCAAAAAA ATCTGAACTG TGGACGGCCC AGGAAACTAT TGTCTATTTG
    GGGGACTACT TGACTGTGAA GAAAAAAGGC AGACAAAGAA ATGCTTTTTG GGTTCATCAT
    CTTCATCAAG AAGAAATTCT GGGGAGGTAT GTTGGGAAAG ACTATAAGGA GCAGAAGGGG
    CTCTGGCACC ACTTCACTGA TGTGGAGCGA CAGATGACCG CACAGCACTA TGTGACAGAA
    TTTAACAAGA GACTCTATGA ACAAAACATT CCCACCCAGA TATTCTACAT CCCATCCACA
    ATACTACTGA TTTTAGAGGA CAAGACAATA AAGGGATGTA TCAGTGTGGA GCCTTACATA
    CTGGGAGAAT TTGTAAAATT GTCAAATAAC ACGAAAGTGG TGAAAACAGA ATACAAAGCC
    ACAGAATATG GCTTGGCCTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT
    GTGGTCGATT TACAAGGTTG GGTAACCGGT AATGGAAAAG GACTCATCTA CCTCACAGAT
    CCCCAGATTC ACTCCGTTGA TCAGAAAGTT TTCACTACCA ATTTTGGAAA GAGAGGAATT
    TTTTACTTCT TTAATAACCA GCATGTGGAA TGTAATGAAA TCTGCCATCG TCTTTCTTTG
    ACTAGACCTT CAATGGAGAA ACCATGTAAG TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95411
    Clone ID Related Accession (Same CDS sequence) XM_017008638.2 , XM_017008636.1 , XM_017008635.1 , XM_017008640.1 , XM_017008638.1 , XM_017008633.1 , XM_017008634.1 , XM_017008637.1 , XM_017008639.1 , XM_017008641.2 , XM_017008641.1
    Accession Version XM_017008633.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3756bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGCCTCC TGTTTTCTCA CAGAGCCTGC TGAAGGAGTT TGACCACCAT
    TTGCTGTCCG CTGCAGAAGC CTGCAAGCTG GCAGCTGCCT TCAGTGCCTA TACGCCGCTC
    TTCGTGCTCA CAGCTGTGAA TATCCGTGGC ACGTGTTTAT TGTCCTACAG TAGTTCAAAT
    GACTGTCCTC CAGAATTGAA AAACTTACAT CTGTGTGAAG CCAAAGAGGC CTTTGAGATT
    GGCCTCCTCA CCAAGAGAGA TGATGAGCCT GTTACTGGAA AACAGGAGCT TCACAGCTTT
    GTCAAAGCTG CTTTCGGTCT CACCACAGTG CACAGAAGGC TCCATGGGGA GACAGGGACG
    GTCCATGCAG CAAGTCAGCT CTGTAAGGAA GCAATGGGGA AGCTGTACAA TTTCAGCACT
    TCCTCCAGAA GTCAGGACAG AGAAGCTCTG TCTCAAGAAG TTATGTCTGT GATTGCCCAG
    GTGAAGGAAC ATTTACAAGT TCAAAGCTTC TCAAATGTAG ATGACAGATC TTATGTTCCC
    GAGAGTTTCG AGTGCAGGTT GGATAAACTT ATCTTGCATG GGCAAGGGGA TTTCCAAAAA
    ATCCTTGACA CCTATTCACA GCACCATACT TCGGTGTGTG AAGTATTTGA AAGTGATTGT
    GGAAACAACA AAAATGAACA GAAAGATGCA AAAACAGGAG TCTGCATCAC TGCTCTAAAA
    ACAGAAATAA AAAACATAGA TACTGTGAGT ACTACTCAAG AAAAGCCACA TTGTCAAAGA
    GACACAGGAA TATCTTCCTC CCTAATGGGT AAGAATGTTC AGAGGGAACT CAGAAGGGGA
    GGAAGGAGAA ACTGGACCCA TTCTGATGCA TTTCGAGTCT CCTTGGATCA AGATGTGGAG
    ACTGAGACTG AGCCATCGGA CTACAGCAAT GGTGAGGGAG CTGTTTTCAA CAAGTCTCTG
    AGTGGCAGCC AGACTTCCAG TGCTTGGAGC AACTTATCAG GGTTTAGTTC CTCTGCAAGC
    TGGGAGGAAG TGAATTATCA CGTTGACGAC AGGTCAGCCA GAAAAGAGCC TGGCAAAGAA
    CATCTGGTGG ACACTCAGTG TTCCACTGCC TTGTCTGAGG AGCTAGAGAA TGACAGGGAA
    GGCAGAGCTA TGCATTCATT GCATTCACAG CTTCATGATC TCTCTCTTCA GGAACCCAAC
    AATGACAATT TGGAGCCTTC TCAAAATCAG CCACAGCAAC AGATGCCCTT GACACCCTTC
    TCGCCTCATA ATACCCCAGG CATTTTCTTG GCCCCTGGTG CAGGGCTTCT AGAAGGAGCT
    CCAGAAGGTA TCCAGGAAGT CAGAAATATG GGACCCAGAA ATACTTCTGC TCACTCCAGA
    CCCTCATATC GTTCTGCTTC TTGGTCTTCT GATTCTGGTA GGCCCAAGAA TATGGGCACA
    CATCCTTCAG TCCAAAAAGA AGAAGCCTTT GAAATAATTG TTGAGTTTCC AGAAACCAAC
    TGCGATGTCA AAGACAGGCA GGGGAAAGAG CAGGGAGAAG AAATTAGTGA AAGAGGCGCA
    GGCCCTACAT TTAAAGCTAG TCCCTCCTGG GTTGACCCAG AAGGAGAAAC AGCAGAAAGC
    ACTGAAGATG CACCCTTAGA CTTTCACAGG GTCCTGCACA ATTCTCTGGG AAACATTTCC
    ATGCTGCCAT GTAGCTCCTT CACCCCTAAT TGGCCTGTTC AAAATCCTGA CTCCAGAAAA
    AGTGGTGGCC CAGTCGCAGA GCAGGGCATC GACCCTGATG CCTCCACAGT GGATGAGGAG
    GGGCAACTGC TCGACAGCAT GGATGTTCCC TGCACAAATG GGCACGGCTC TCATAGACTG
    TGCATTCTGA GACAGCCGCC TGGTCAGAGG GCGGAGACCC CCAATTCCTC TGTAAGCGGT
    AACATCCTCT TCCCTGTCCT CAGCGAGGAC TGCACTACCA CAGAGGAAGG AAATCAGCCT
    GGAAACATGC TAAACTGCAG CCAGAACTCC AGCTCATCCT CAGTGTGGTG GCTGAAATCA
    CCTGCATTTT CCAGTGGTTC TTCTGAGGGG GACAGCCCTT GGTCCTATCT GAATTCCAGT
    GGGAGTTCTT GGGTTTCATT GCCGGGAAAG ATGAGGAAAG AGATCCTTGA GGCTCGCACC
    TTGCAACCTG ATGACTTTGA AAAGCTGTTG GCAGGAGTGA GGCATGATTG GCTGTTTCAG
    AGACTAGAGA ATACGGGGGT TTTTAAGCCC AGTCAACTCC ACCGAGCACA TAGTGCTCTT
    TTGTTAAAAT ATTCAAAAAA ATCTGAACTG TGGACGGCCC AGGAAACTAT TGTCTATTTG
    GGGGACTACT TGACTGTGAA GAAAAAAGGC AGACAAAGAA ATGCTTTTTG GGTTCATCAT
    CTTCATCAAG AAGAAATTCT GGGGAGGTAT GTTGGGAAAG ACTATAAGGA GCAGAAGGGG
    CTCTGGCACC ACTTCACTGA TGTGGAGCGA CAGATGACCG CACAGCACTA TGTGACAGAA
    TTTAACAAGA GACTCTATGA ACAAAACATT CCCACCCAGA TATTCTACAT CCCATCCACA
    ATACTACTGA TTTTAGAGGA CAAGACAATA AAGGGATGTA TCAGTGTGGA GCCTTACATA
    CTGGGAGAAT TTGTAAAATT GTCAAATAAC ACGAAAGTGG TGAAAACAGA ATACAAAGCC
    ACAGAATATG GCTTGGCCTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT
    GTGGTCGATT TACAAGGTTG GGTAACCGGT AATGGAAAAG GACTCATCTA CCTCACAGAT
    CCCCAGATTC ACTCCGTTGA TCAGAAAGTT TTCACTACCA ATTTTGGAAA GAGAGGAATT
    TTTTACTTCT TTAATAACCA GCATGTGGAA TGTAATGAAA TCTGCCATCG TCTTTCTTTG
    ACTAGACCTT CAATGGAGAA ACCATGTAAG TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864122.1
    CDS280..4035
    Translation

    Target ORF information:

    RefSeq Version XM_017008633.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008633.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGCCTCC TGTTTTCTCA CAGAGCCTGC TGAAGGAGTT TGACCACCAT
    TTGCTGTCCG CTGCAGAAGC CTGCAAGCTG GCAGCTGCCT TCAGTGCCTA TACGCCGCTC
    TTCGTGCTCA CAGCTGTGAA TATCCGTGGC ACGTGTTTAT TGTCCTACAG TAGTTCAAAT
    GACTGTCCTC CAGAATTGAA AAACTTACAT CTGTGTGAAG CCAAAGAGGC CTTTGAGATT
    GGCCTCCTCA CCAAGAGAGA TGATGAGCCT GTTACTGGAA AACAGGAGCT TCACAGCTTT
    GTCAAAGCTG CTTTCGGTCT CACCACAGTG CACAGAAGGC TCCATGGGGA GACAGGGACG
    GTCCATGCAG CAAGTCAGCT CTGTAAGGAA GCAATGGGGA AGCTGTACAA TTTCAGCACT
    TCCTCCAGAA GTCAGGACAG AGAAGCTCTG TCTCAAGAAG TTATGTCTGT GATTGCCCAG
    GTGAAGGAAC ATTTACAAGT TCAAAGCTTC TCAAATGTAG ATGACAGATC TTATGTTCCC
    GAGAGTTTCG AGTGCAGGTT GGATAAACTT ATCTTGCATG GGCAAGGGGA TTTCCAAAAA
    ATCCTTGACA CCTATTCACA GCACCATACT TCGGTGTGTG AAGTATTTGA AAGTGATTGT
    GGAAACAACA AAAATGAACA GAAAGATGCA AAAACAGGAG TCTGCATCAC TGCTCTAAAA
    ACAGAAATAA AAAACATAGA TACTGTGAGT ACTACTCAAG AAAAGCCACA TTGTCAAAGA
    GACACAGGAA TATCTTCCTC CCTAATGGGT AAGAATGTTC AGAGGGAACT CAGAAGGGGA
    GGAAGGAGAA ACTGGACCCA TTCTGATGCA TTTCGAGTCT CCTTGGATCA AGATGTGGAG
    ACTGAGACTG AGCCATCGGA CTACAGCAAT GGTGAGGGAG CTGTTTTCAA CAAGTCTCTG
    AGTGGCAGCC AGACTTCCAG TGCTTGGAGC AACTTATCAG GGTTTAGTTC CTCTGCAAGC
    TGGGAGGAAG TGAATTATCA CGTTGACGAC AGGTCAGCCA GAAAAGAGCC TGGCAAAGAA
    CATCTGGTGG ACACTCAGTG TTCCACTGCC TTGTCTGAGG AGCTAGAGAA TGACAGGGAA
    GGCAGAGCTA TGCATTCATT GCATTCACAG CTTCATGATC TCTCTCTTCA GGAACCCAAC
    AATGACAATT TGGAGCCTTC TCAAAATCAG CCACAGCAAC AGATGCCCTT GACACCCTTC
    TCGCCTCATA ATACCCCAGG CATTTTCTTG GCCCCTGGTG CAGGGCTTCT AGAAGGAGCT
    CCAGAAGGTA TCCAGGAAGT CAGAAATATG GGACCCAGAA ATACTTCTGC TCACTCCAGA
    CCCTCATATC GTTCTGCTTC TTGGTCTTCT GATTCTGGTA GGCCCAAGAA TATGGGCACA
    CATCCTTCAG TCCAAAAAGA AGAAGCCTTT GAAATAATTG TTGAGTTTCC AGAAACCAAC
    TGCGATGTCA AAGACAGGCA GGGGAAAGAG CAGGGAGAAG AAATTAGTGA AAGAGGCGCA
    GGCCCTACAT TTAAAGCTAG TCCCTCCTGG GTTGACCCAG AAGGAGAAAC AGCAGAAAGC
    ACTGAAGATG CACCCTTAGA CTTTCACAGG GTCCTGCACA ATTCTCTGGG AAACATTTCC
    ATGCTGCCAT GTAGCTCCTT CACCCCTAAT TGGCCTGTTC AAAATCCTGA CTCCAGAAAA
    AGTGGTGGCC CAGTCGCAGA GCAGGGCATC GACCCTGATG CCTCCACAGT GGATGAGGAG
    GGGCAACTGC TCGACAGCAT GGATGTTCCC TGCACAAATG GGCACGGCTC TCATAGACTG
    TGCATTCTGA GACAGCCGCC TGGTCAGAGG GCGGAGACCC CCAATTCCTC TGTAAGCGGT
    AACATCCTCT TCCCTGTCCT CAGCGAGGAC TGCACTACCA CAGAGGAAGG AAATCAGCCT
    GGAAACATGC TAAACTGCAG CCAGAACTCC AGCTCATCCT CAGTGTGGTG GCTGAAATCA
    CCTGCATTTT CCAGTGGTTC TTCTGAGGGG GACAGCCCTT GGTCCTATCT GAATTCCAGT
    GGGAGTTCTT GGGTTTCATT GCCGGGAAAG ATGAGGAAAG AGATCCTTGA GGCTCGCACC
    TTGCAACCTG ATGACTTTGA AAAGCTGTTG GCAGGAGTGA GGCATGATTG GCTGTTTCAG
    AGACTAGAGA ATACGGGGGT TTTTAAGCCC AGTCAACTCC ACCGAGCACA TAGTGCTCTT
    TTGTTAAAAT ATTCAAAAAA ATCTGAACTG TGGACGGCCC AGGAAACTAT TGTCTATTTG
    GGGGACTACT TGACTGTGAA GAAAAAAGGC AGACAAAGAA ATGCTTTTTG GGTTCATCAT
    CTTCATCAAG AAGAAATTCT GGGGAGGTAT GTTGGGAAAG ACTATAAGGA GCAGAAGGGG
    CTCTGGCACC ACTTCACTGA TGTGGAGCGA CAGATGACCG CACAGCACTA TGTGACAGAA
    TTTAACAAGA GACTCTATGA ACAAAACATT CCCACCCAGA TATTCTACAT CCCATCCACA
    ATACTACTGA TTTTAGAGGA CAAGACAATA AAGGGATGTA TCAGTGTGGA GCCTTACATA
    CTGGGAGAAT TTGTAAAATT GTCAAATAAC ACGAAAGTGG TGAAAACAGA ATACAAAGCC
    ACAGAATATG GCTTGGCCTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT
    GTGGTCGATT TACAAGGTTG GGTAACCGGT AATGGAAAAG GACTCATCTA CCTCACAGAT
    CCCCAGATTC ACTCCGTTGA TCAGAAAGTT TTCACTACCA ATTTTGGAAA GAGAGGAATT
    TTTTACTTCT TTAATAACCA GCATGTGGAA TGTAATGAAA TCTGCCATCG TCTTTCTTTG
    ACTAGACCTT CAATGGAGAA ACCATGTAAG TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95411
    Clone ID Related Accession (Same CDS sequence) XM_017008638.2 , XM_017008636.1 , XM_017008635.1 , XM_017008640.1 , XM_017008638.1 , XM_017008633.1 , XM_017008634.1 , XM_017008637.1 , XM_017008639.1 , XM_017008641.2 , XM_017008641.1
    Accession Version XM_017008634.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3756bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGCCTCC TGTTTTCTCA CAGAGCCTGC TGAAGGAGTT TGACCACCAT
    TTGCTGTCCG CTGCAGAAGC CTGCAAGCTG GCAGCTGCCT TCAGTGCCTA TACGCCGCTC
    TTCGTGCTCA CAGCTGTGAA TATCCGTGGC ACGTGTTTAT TGTCCTACAG TAGTTCAAAT
    GACTGTCCTC CAGAATTGAA AAACTTACAT CTGTGTGAAG CCAAAGAGGC CTTTGAGATT
    GGCCTCCTCA CCAAGAGAGA TGATGAGCCT GTTACTGGAA AACAGGAGCT TCACAGCTTT
    GTCAAAGCTG CTTTCGGTCT CACCACAGTG CACAGAAGGC TCCATGGGGA GACAGGGACG
    GTCCATGCAG CAAGTCAGCT CTGTAAGGAA GCAATGGGGA AGCTGTACAA TTTCAGCACT
    TCCTCCAGAA GTCAGGACAG AGAAGCTCTG TCTCAAGAAG TTATGTCTGT GATTGCCCAG
    GTGAAGGAAC ATTTACAAGT TCAAAGCTTC TCAAATGTAG ATGACAGATC TTATGTTCCC
    GAGAGTTTCG AGTGCAGGTT GGATAAACTT ATCTTGCATG GGCAAGGGGA TTTCCAAAAA
    ATCCTTGACA CCTATTCACA GCACCATACT TCGGTGTGTG AAGTATTTGA AAGTGATTGT
    GGAAACAACA AAAATGAACA GAAAGATGCA AAAACAGGAG TCTGCATCAC TGCTCTAAAA
    ACAGAAATAA AAAACATAGA TACTGTGAGT ACTACTCAAG AAAAGCCACA TTGTCAAAGA
    GACACAGGAA TATCTTCCTC CCTAATGGGT AAGAATGTTC AGAGGGAACT CAGAAGGGGA
    GGAAGGAGAA ACTGGACCCA TTCTGATGCA TTTCGAGTCT CCTTGGATCA AGATGTGGAG
    ACTGAGACTG AGCCATCGGA CTACAGCAAT GGTGAGGGAG CTGTTTTCAA CAAGTCTCTG
    AGTGGCAGCC AGACTTCCAG TGCTTGGAGC AACTTATCAG GGTTTAGTTC CTCTGCAAGC
    TGGGAGGAAG TGAATTATCA CGTTGACGAC AGGTCAGCCA GAAAAGAGCC TGGCAAAGAA
    CATCTGGTGG ACACTCAGTG TTCCACTGCC TTGTCTGAGG AGCTAGAGAA TGACAGGGAA
    GGCAGAGCTA TGCATTCATT GCATTCACAG CTTCATGATC TCTCTCTTCA GGAACCCAAC
    AATGACAATT TGGAGCCTTC TCAAAATCAG CCACAGCAAC AGATGCCCTT GACACCCTTC
    TCGCCTCATA ATACCCCAGG CATTTTCTTG GCCCCTGGTG CAGGGCTTCT AGAAGGAGCT
    CCAGAAGGTA TCCAGGAAGT CAGAAATATG GGACCCAGAA ATACTTCTGC TCACTCCAGA
    CCCTCATATC GTTCTGCTTC TTGGTCTTCT GATTCTGGTA GGCCCAAGAA TATGGGCACA
    CATCCTTCAG TCCAAAAAGA AGAAGCCTTT GAAATAATTG TTGAGTTTCC AGAAACCAAC
    TGCGATGTCA AAGACAGGCA GGGGAAAGAG CAGGGAGAAG AAATTAGTGA AAGAGGCGCA
    GGCCCTACAT TTAAAGCTAG TCCCTCCTGG GTTGACCCAG AAGGAGAAAC AGCAGAAAGC
    ACTGAAGATG CACCCTTAGA CTTTCACAGG GTCCTGCACA ATTCTCTGGG AAACATTTCC
    ATGCTGCCAT GTAGCTCCTT CACCCCTAAT TGGCCTGTTC AAAATCCTGA CTCCAGAAAA
    AGTGGTGGCC CAGTCGCAGA GCAGGGCATC GACCCTGATG CCTCCACAGT GGATGAGGAG
    GGGCAACTGC TCGACAGCAT GGATGTTCCC TGCACAAATG GGCACGGCTC TCATAGACTG
    TGCATTCTGA GACAGCCGCC TGGTCAGAGG GCGGAGACCC CCAATTCCTC TGTAAGCGGT
    AACATCCTCT TCCCTGTCCT CAGCGAGGAC TGCACTACCA CAGAGGAAGG AAATCAGCCT
    GGAAACATGC TAAACTGCAG CCAGAACTCC AGCTCATCCT CAGTGTGGTG GCTGAAATCA
    CCTGCATTTT CCAGTGGTTC TTCTGAGGGG GACAGCCCTT GGTCCTATCT GAATTCCAGT
    GGGAGTTCTT GGGTTTCATT GCCGGGAAAG ATGAGGAAAG AGATCCTTGA GGCTCGCACC
    TTGCAACCTG ATGACTTTGA AAAGCTGTTG GCAGGAGTGA GGCATGATTG GCTGTTTCAG
    AGACTAGAGA ATACGGGGGT TTTTAAGCCC AGTCAACTCC ACCGAGCACA TAGTGCTCTT
    TTGTTAAAAT ATTCAAAAAA ATCTGAACTG TGGACGGCCC AGGAAACTAT TGTCTATTTG
    GGGGACTACT TGACTGTGAA GAAAAAAGGC AGACAAAGAA ATGCTTTTTG GGTTCATCAT
    CTTCATCAAG AAGAAATTCT GGGGAGGTAT GTTGGGAAAG ACTATAAGGA GCAGAAGGGG
    CTCTGGCACC ACTTCACTGA TGTGGAGCGA CAGATGACCG CACAGCACTA TGTGACAGAA
    TTTAACAAGA GACTCTATGA ACAAAACATT CCCACCCAGA TATTCTACAT CCCATCCACA
    ATACTACTGA TTTTAGAGGA CAAGACAATA AAGGGATGTA TCAGTGTGGA GCCTTACATA
    CTGGGAGAAT TTGTAAAATT GTCAAATAAC ACGAAAGTGG TGAAAACAGA ATACAAAGCC
    ACAGAATATG GCTTGGCCTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT
    GTGGTCGATT TACAAGGTTG GGTAACCGGT AATGGAAAAG GACTCATCTA CCTCACAGAT
    CCCCAGATTC ACTCCGTTGA TCAGAAAGTT TTCACTACCA ATTTTGGAAA GAGAGGAATT
    TTTTACTTCT TTAATAACCA GCATGTGGAA TGTAATGAAA TCTGCCATCG TCTTTCTTTG
    ACTAGACCTT CAATGGAGAA ACCATGTAAG TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864123.1
    CDS248..4003
    Translation

    Target ORF information:

    RefSeq Version XM_017008634.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008634.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGCCTCC TGTTTTCTCA CAGAGCCTGC TGAAGGAGTT TGACCACCAT
    TTGCTGTCCG CTGCAGAAGC CTGCAAGCTG GCAGCTGCCT TCAGTGCCTA TACGCCGCTC
    TTCGTGCTCA CAGCTGTGAA TATCCGTGGC ACGTGTTTAT TGTCCTACAG TAGTTCAAAT
    GACTGTCCTC CAGAATTGAA AAACTTACAT CTGTGTGAAG CCAAAGAGGC CTTTGAGATT
    GGCCTCCTCA CCAAGAGAGA TGATGAGCCT GTTACTGGAA AACAGGAGCT TCACAGCTTT
    GTCAAAGCTG CTTTCGGTCT CACCACAGTG CACAGAAGGC TCCATGGGGA GACAGGGACG
    GTCCATGCAG CAAGTCAGCT CTGTAAGGAA GCAATGGGGA AGCTGTACAA TTTCAGCACT
    TCCTCCAGAA GTCAGGACAG AGAAGCTCTG TCTCAAGAAG TTATGTCTGT GATTGCCCAG
    GTGAAGGAAC ATTTACAAGT TCAAAGCTTC TCAAATGTAG ATGACAGATC TTATGTTCCC
    GAGAGTTTCG AGTGCAGGTT GGATAAACTT ATCTTGCATG GGCAAGGGGA TTTCCAAAAA
    ATCCTTGACA CCTATTCACA GCACCATACT TCGGTGTGTG AAGTATTTGA AAGTGATTGT
    GGAAACAACA AAAATGAACA GAAAGATGCA AAAACAGGAG TCTGCATCAC TGCTCTAAAA
    ACAGAAATAA AAAACATAGA TACTGTGAGT ACTACTCAAG AAAAGCCACA TTGTCAAAGA
    GACACAGGAA TATCTTCCTC CCTAATGGGT AAGAATGTTC AGAGGGAACT CAGAAGGGGA
    GGAAGGAGAA ACTGGACCCA TTCTGATGCA TTTCGAGTCT CCTTGGATCA AGATGTGGAG
    ACTGAGACTG AGCCATCGGA CTACAGCAAT GGTGAGGGAG CTGTTTTCAA CAAGTCTCTG
    AGTGGCAGCC AGACTTCCAG TGCTTGGAGC AACTTATCAG GGTTTAGTTC CTCTGCAAGC
    TGGGAGGAAG TGAATTATCA CGTTGACGAC AGGTCAGCCA GAAAAGAGCC TGGCAAAGAA
    CATCTGGTGG ACACTCAGTG TTCCACTGCC TTGTCTGAGG AGCTAGAGAA TGACAGGGAA
    GGCAGAGCTA TGCATTCATT GCATTCACAG CTTCATGATC TCTCTCTTCA GGAACCCAAC
    AATGACAATT TGGAGCCTTC TCAAAATCAG CCACAGCAAC AGATGCCCTT GACACCCTTC
    TCGCCTCATA ATACCCCAGG CATTTTCTTG GCCCCTGGTG CAGGGCTTCT AGAAGGAGCT
    CCAGAAGGTA TCCAGGAAGT CAGAAATATG GGACCCAGAA ATACTTCTGC TCACTCCAGA
    CCCTCATATC GTTCTGCTTC TTGGTCTTCT GATTCTGGTA GGCCCAAGAA TATGGGCACA
    CATCCTTCAG TCCAAAAAGA AGAAGCCTTT GAAATAATTG TTGAGTTTCC AGAAACCAAC
    TGCGATGTCA AAGACAGGCA GGGGAAAGAG CAGGGAGAAG AAATTAGTGA AAGAGGCGCA
    GGCCCTACAT TTAAAGCTAG TCCCTCCTGG GTTGACCCAG AAGGAGAAAC AGCAGAAAGC
    ACTGAAGATG CACCCTTAGA CTTTCACAGG GTCCTGCACA ATTCTCTGGG AAACATTTCC
    ATGCTGCCAT GTAGCTCCTT CACCCCTAAT TGGCCTGTTC AAAATCCTGA CTCCAGAAAA
    AGTGGTGGCC CAGTCGCAGA GCAGGGCATC GACCCTGATG CCTCCACAGT GGATGAGGAG
    GGGCAACTGC TCGACAGCAT GGATGTTCCC TGCACAAATG GGCACGGCTC TCATAGACTG
    TGCATTCTGA GACAGCCGCC TGGTCAGAGG GCGGAGACCC CCAATTCCTC TGTAAGCGGT
    AACATCCTCT TCCCTGTCCT CAGCGAGGAC TGCACTACCA CAGAGGAAGG AAATCAGCCT
    GGAAACATGC TAAACTGCAG CCAGAACTCC AGCTCATCCT CAGTGTGGTG GCTGAAATCA
    CCTGCATTTT CCAGTGGTTC TTCTGAGGGG GACAGCCCTT GGTCCTATCT GAATTCCAGT
    GGGAGTTCTT GGGTTTCATT GCCGGGAAAG ATGAGGAAAG AGATCCTTGA GGCTCGCACC
    TTGCAACCTG ATGACTTTGA AAAGCTGTTG GCAGGAGTGA GGCATGATTG GCTGTTTCAG
    AGACTAGAGA ATACGGGGGT TTTTAAGCCC AGTCAACTCC ACCGAGCACA TAGTGCTCTT
    TTGTTAAAAT ATTCAAAAAA ATCTGAACTG TGGACGGCCC AGGAAACTAT TGTCTATTTG
    GGGGACTACT TGACTGTGAA GAAAAAAGGC AGACAAAGAA ATGCTTTTTG GGTTCATCAT
    CTTCATCAAG AAGAAATTCT GGGGAGGTAT GTTGGGAAAG ACTATAAGGA GCAGAAGGGG
    CTCTGGCACC ACTTCACTGA TGTGGAGCGA CAGATGACCG CACAGCACTA TGTGACAGAA
    TTTAACAAGA GACTCTATGA ACAAAACATT CCCACCCAGA TATTCTACAT CCCATCCACA
    ATACTACTGA TTTTAGAGGA CAAGACAATA AAGGGATGTA TCAGTGTGGA GCCTTACATA
    CTGGGAGAAT TTGTAAAATT GTCAAATAAC ACGAAAGTGG TGAAAACAGA ATACAAAGCC
    ACAGAATATG GCTTGGCCTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT
    GTGGTCGATT TACAAGGTTG GGTAACCGGT AATGGAAAAG GACTCATCTA CCTCACAGAT
    CCCCAGATTC ACTCCGTTGA TCAGAAAGTT TTCACTACCA ATTTTGGAAA GAGAGGAATT
    TTTTACTTCT TTAATAACCA GCATGTGGAA TGTAATGAAA TCTGCCATCG TCTTTCTTTG
    ACTAGACCTT CAATGGAGAA ACCATGTAAG TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95411
    Clone ID Related Accession (Same CDS sequence) XM_017008638.2 , XM_017008636.1 , XM_017008635.1 , XM_017008640.1 , XM_017008638.1 , XM_017008633.1 , XM_017008634.1 , XM_017008637.1 , XM_017008639.1 , XM_017008641.2 , XM_017008641.1
    Accession Version XM_017008637.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3756bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGCCTCC TGTTTTCTCA CAGAGCCTGC TGAAGGAGTT TGACCACCAT
    TTGCTGTCCG CTGCAGAAGC CTGCAAGCTG GCAGCTGCCT TCAGTGCCTA TACGCCGCTC
    TTCGTGCTCA CAGCTGTGAA TATCCGTGGC ACGTGTTTAT TGTCCTACAG TAGTTCAAAT
    GACTGTCCTC CAGAATTGAA AAACTTACAT CTGTGTGAAG CCAAAGAGGC CTTTGAGATT
    GGCCTCCTCA CCAAGAGAGA TGATGAGCCT GTTACTGGAA AACAGGAGCT TCACAGCTTT
    GTCAAAGCTG CTTTCGGTCT CACCACAGTG CACAGAAGGC TCCATGGGGA GACAGGGACG
    GTCCATGCAG CAAGTCAGCT CTGTAAGGAA GCAATGGGGA AGCTGTACAA TTTCAGCACT
    TCCTCCAGAA GTCAGGACAG AGAAGCTCTG TCTCAAGAAG TTATGTCTGT GATTGCCCAG
    GTGAAGGAAC ATTTACAAGT TCAAAGCTTC TCAAATGTAG ATGACAGATC TTATGTTCCC
    GAGAGTTTCG AGTGCAGGTT GGATAAACTT ATCTTGCATG GGCAAGGGGA TTTCCAAAAA
    ATCCTTGACA CCTATTCACA GCACCATACT TCGGTGTGTG AAGTATTTGA AAGTGATTGT
    GGAAACAACA AAAATGAACA GAAAGATGCA AAAACAGGAG TCTGCATCAC TGCTCTAAAA
    ACAGAAATAA AAAACATAGA TACTGTGAGT ACTACTCAAG AAAAGCCACA TTGTCAAAGA
    GACACAGGAA TATCTTCCTC CCTAATGGGT AAGAATGTTC AGAGGGAACT CAGAAGGGGA
    GGAAGGAGAA ACTGGACCCA TTCTGATGCA TTTCGAGTCT CCTTGGATCA AGATGTGGAG
    ACTGAGACTG AGCCATCGGA CTACAGCAAT GGTGAGGGAG CTGTTTTCAA CAAGTCTCTG
    AGTGGCAGCC AGACTTCCAG TGCTTGGAGC AACTTATCAG GGTTTAGTTC CTCTGCAAGC
    TGGGAGGAAG TGAATTATCA CGTTGACGAC AGGTCAGCCA GAAAAGAGCC TGGCAAAGAA
    CATCTGGTGG ACACTCAGTG TTCCACTGCC TTGTCTGAGG AGCTAGAGAA TGACAGGGAA
    GGCAGAGCTA TGCATTCATT GCATTCACAG CTTCATGATC TCTCTCTTCA GGAACCCAAC
    AATGACAATT TGGAGCCTTC TCAAAATCAG CCACAGCAAC AGATGCCCTT GACACCCTTC
    TCGCCTCATA ATACCCCAGG CATTTTCTTG GCCCCTGGTG CAGGGCTTCT AGAAGGAGCT
    CCAGAAGGTA TCCAGGAAGT CAGAAATATG GGACCCAGAA ATACTTCTGC TCACTCCAGA
    CCCTCATATC GTTCTGCTTC TTGGTCTTCT GATTCTGGTA GGCCCAAGAA TATGGGCACA
    CATCCTTCAG TCCAAAAAGA AGAAGCCTTT GAAATAATTG TTGAGTTTCC AGAAACCAAC
    TGCGATGTCA AAGACAGGCA GGGGAAAGAG CAGGGAGAAG AAATTAGTGA AAGAGGCGCA
    GGCCCTACAT TTAAAGCTAG TCCCTCCTGG GTTGACCCAG AAGGAGAAAC AGCAGAAAGC
    ACTGAAGATG CACCCTTAGA CTTTCACAGG GTCCTGCACA ATTCTCTGGG AAACATTTCC
    ATGCTGCCAT GTAGCTCCTT CACCCCTAAT TGGCCTGTTC AAAATCCTGA CTCCAGAAAA
    AGTGGTGGCC CAGTCGCAGA GCAGGGCATC GACCCTGATG CCTCCACAGT GGATGAGGAG
    GGGCAACTGC TCGACAGCAT GGATGTTCCC TGCACAAATG GGCACGGCTC TCATAGACTG
    TGCATTCTGA GACAGCCGCC TGGTCAGAGG GCGGAGACCC CCAATTCCTC TGTAAGCGGT
    AACATCCTCT TCCCTGTCCT CAGCGAGGAC TGCACTACCA CAGAGGAAGG AAATCAGCCT
    GGAAACATGC TAAACTGCAG CCAGAACTCC AGCTCATCCT CAGTGTGGTG GCTGAAATCA
    CCTGCATTTT CCAGTGGTTC TTCTGAGGGG GACAGCCCTT GGTCCTATCT GAATTCCAGT
    GGGAGTTCTT GGGTTTCATT GCCGGGAAAG ATGAGGAAAG AGATCCTTGA GGCTCGCACC
    TTGCAACCTG ATGACTTTGA AAAGCTGTTG GCAGGAGTGA GGCATGATTG GCTGTTTCAG
    AGACTAGAGA ATACGGGGGT TTTTAAGCCC AGTCAACTCC ACCGAGCACA TAGTGCTCTT
    TTGTTAAAAT ATTCAAAAAA ATCTGAACTG TGGACGGCCC AGGAAACTAT TGTCTATTTG
    GGGGACTACT TGACTGTGAA GAAAAAAGGC AGACAAAGAA ATGCTTTTTG GGTTCATCAT
    CTTCATCAAG AAGAAATTCT GGGGAGGTAT GTTGGGAAAG ACTATAAGGA GCAGAAGGGG
    CTCTGGCACC ACTTCACTGA TGTGGAGCGA CAGATGACCG CACAGCACTA TGTGACAGAA
    TTTAACAAGA GACTCTATGA ACAAAACATT CCCACCCAGA TATTCTACAT CCCATCCACA
    ATACTACTGA TTTTAGAGGA CAAGACAATA AAGGGATGTA TCAGTGTGGA GCCTTACATA
    CTGGGAGAAT TTGTAAAATT GTCAAATAAC ACGAAAGTGG TGAAAACAGA ATACAAAGCC
    ACAGAATATG GCTTGGCCTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT
    GTGGTCGATT TACAAGGTTG GGTAACCGGT AATGGAAAAG GACTCATCTA CCTCACAGAT
    CCCCAGATTC ACTCCGTTGA TCAGAAAGTT TTCACTACCA ATTTTGGAAA GAGAGGAATT
    TTTTACTTCT TTAATAACCA GCATGTGGAA TGTAATGAAA TCTGCCATCG TCTTTCTTTG
    ACTAGACCTT CAATGGAGAA ACCATGTAAG TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864126.1
    CDS231..3986
    Translation

    Target ORF information:

    RefSeq Version XM_017008637.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008637.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGCCTCC TGTTTTCTCA CAGAGCCTGC TGAAGGAGTT TGACCACCAT
    TTGCTGTCCG CTGCAGAAGC CTGCAAGCTG GCAGCTGCCT TCAGTGCCTA TACGCCGCTC
    TTCGTGCTCA CAGCTGTGAA TATCCGTGGC ACGTGTTTAT TGTCCTACAG TAGTTCAAAT
    GACTGTCCTC CAGAATTGAA AAACTTACAT CTGTGTGAAG CCAAAGAGGC CTTTGAGATT
    GGCCTCCTCA CCAAGAGAGA TGATGAGCCT GTTACTGGAA AACAGGAGCT TCACAGCTTT
    GTCAAAGCTG CTTTCGGTCT CACCACAGTG CACAGAAGGC TCCATGGGGA GACAGGGACG
    GTCCATGCAG CAAGTCAGCT CTGTAAGGAA GCAATGGGGA AGCTGTACAA TTTCAGCACT
    TCCTCCAGAA GTCAGGACAG AGAAGCTCTG TCTCAAGAAG TTATGTCTGT GATTGCCCAG
    GTGAAGGAAC ATTTACAAGT TCAAAGCTTC TCAAATGTAG ATGACAGATC TTATGTTCCC
    GAGAGTTTCG AGTGCAGGTT GGATAAACTT ATCTTGCATG GGCAAGGGGA TTTCCAAAAA
    ATCCTTGACA CCTATTCACA GCACCATACT TCGGTGTGTG AAGTATTTGA AAGTGATTGT
    GGAAACAACA AAAATGAACA GAAAGATGCA AAAACAGGAG TCTGCATCAC TGCTCTAAAA
    ACAGAAATAA AAAACATAGA TACTGTGAGT ACTACTCAAG AAAAGCCACA TTGTCAAAGA
    GACACAGGAA TATCTTCCTC CCTAATGGGT AAGAATGTTC AGAGGGAACT CAGAAGGGGA
    GGAAGGAGAA ACTGGACCCA TTCTGATGCA TTTCGAGTCT CCTTGGATCA AGATGTGGAG
    ACTGAGACTG AGCCATCGGA CTACAGCAAT GGTGAGGGAG CTGTTTTCAA CAAGTCTCTG
    AGTGGCAGCC AGACTTCCAG TGCTTGGAGC AACTTATCAG GGTTTAGTTC CTCTGCAAGC
    TGGGAGGAAG TGAATTATCA CGTTGACGAC AGGTCAGCCA GAAAAGAGCC TGGCAAAGAA
    CATCTGGTGG ACACTCAGTG TTCCACTGCC TTGTCTGAGG AGCTAGAGAA TGACAGGGAA
    GGCAGAGCTA TGCATTCATT GCATTCACAG CTTCATGATC TCTCTCTTCA GGAACCCAAC
    AATGACAATT TGGAGCCTTC TCAAAATCAG CCACAGCAAC AGATGCCCTT GACACCCTTC
    TCGCCTCATA ATACCCCAGG CATTTTCTTG GCCCCTGGTG CAGGGCTTCT AGAAGGAGCT
    CCAGAAGGTA TCCAGGAAGT CAGAAATATG GGACCCAGAA ATACTTCTGC TCACTCCAGA
    CCCTCATATC GTTCTGCTTC TTGGTCTTCT GATTCTGGTA GGCCCAAGAA TATGGGCACA
    CATCCTTCAG TCCAAAAAGA AGAAGCCTTT GAAATAATTG TTGAGTTTCC AGAAACCAAC
    TGCGATGTCA AAGACAGGCA GGGGAAAGAG CAGGGAGAAG AAATTAGTGA AAGAGGCGCA
    GGCCCTACAT TTAAAGCTAG TCCCTCCTGG GTTGACCCAG AAGGAGAAAC AGCAGAAAGC
    ACTGAAGATG CACCCTTAGA CTTTCACAGG GTCCTGCACA ATTCTCTGGG AAACATTTCC
    ATGCTGCCAT GTAGCTCCTT CACCCCTAAT TGGCCTGTTC AAAATCCTGA CTCCAGAAAA
    AGTGGTGGCC CAGTCGCAGA GCAGGGCATC GACCCTGATG CCTCCACAGT GGATGAGGAG
    GGGCAACTGC TCGACAGCAT GGATGTTCCC TGCACAAATG GGCACGGCTC TCATAGACTG
    TGCATTCTGA GACAGCCGCC TGGTCAGAGG GCGGAGACCC CCAATTCCTC TGTAAGCGGT
    AACATCCTCT TCCCTGTCCT CAGCGAGGAC TGCACTACCA CAGAGGAAGG AAATCAGCCT
    GGAAACATGC TAAACTGCAG CCAGAACTCC AGCTCATCCT CAGTGTGGTG GCTGAAATCA
    CCTGCATTTT CCAGTGGTTC TTCTGAGGGG GACAGCCCTT GGTCCTATCT GAATTCCAGT
    GGGAGTTCTT GGGTTTCATT GCCGGGAAAG ATGAGGAAAG AGATCCTTGA GGCTCGCACC
    TTGCAACCTG ATGACTTTGA AAAGCTGTTG GCAGGAGTGA GGCATGATTG GCTGTTTCAG
    AGACTAGAGA ATACGGGGGT TTTTAAGCCC AGTCAACTCC ACCGAGCACA TAGTGCTCTT
    TTGTTAAAAT ATTCAAAAAA ATCTGAACTG TGGACGGCCC AGGAAACTAT TGTCTATTTG
    GGGGACTACT TGACTGTGAA GAAAAAAGGC AGACAAAGAA ATGCTTTTTG GGTTCATCAT
    CTTCATCAAG AAGAAATTCT GGGGAGGTAT GTTGGGAAAG ACTATAAGGA GCAGAAGGGG
    CTCTGGCACC ACTTCACTGA TGTGGAGCGA CAGATGACCG CACAGCACTA TGTGACAGAA
    TTTAACAAGA GACTCTATGA ACAAAACATT CCCACCCAGA TATTCTACAT CCCATCCACA
    ATACTACTGA TTTTAGAGGA CAAGACAATA AAGGGATGTA TCAGTGTGGA GCCTTACATA
    CTGGGAGAAT TTGTAAAATT GTCAAATAAC ACGAAAGTGG TGAAAACAGA ATACAAAGCC
    ACAGAATATG GCTTGGCCTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT
    GTGGTCGATT TACAAGGTTG GGTAACCGGT AATGGAAAAG GACTCATCTA CCTCACAGAT
    CCCCAGATTC ACTCCGTTGA TCAGAAAGTT TTCACTACCA ATTTTGGAAA GAGAGGAATT
    TTTTACTTCT TTAATAACCA GCATGTGGAA TGTAATGAAA TCTGCCATCG TCTTTCTTTG
    ACTAGACCTT CAATGGAGAA ACCATGTAAG TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95411
    Clone ID Related Accession (Same CDS sequence) XM_017008638.2 , XM_017008636.1 , XM_017008635.1 , XM_017008640.1 , XM_017008638.1 , XM_017008633.1 , XM_017008634.1 , XM_017008637.1 , XM_017008639.1 , XM_017008641.2 , XM_017008641.1
    Accession Version XM_017008639.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3756bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGCCTCC TGTTTTCTCA CAGAGCCTGC TGAAGGAGTT TGACCACCAT
    TTGCTGTCCG CTGCAGAAGC CTGCAAGCTG GCAGCTGCCT TCAGTGCCTA TACGCCGCTC
    TTCGTGCTCA CAGCTGTGAA TATCCGTGGC ACGTGTTTAT TGTCCTACAG TAGTTCAAAT
    GACTGTCCTC CAGAATTGAA AAACTTACAT CTGTGTGAAG CCAAAGAGGC CTTTGAGATT
    GGCCTCCTCA CCAAGAGAGA TGATGAGCCT GTTACTGGAA AACAGGAGCT TCACAGCTTT
    GTCAAAGCTG CTTTCGGTCT CACCACAGTG CACAGAAGGC TCCATGGGGA GACAGGGACG
    GTCCATGCAG CAAGTCAGCT CTGTAAGGAA GCAATGGGGA AGCTGTACAA TTTCAGCACT
    TCCTCCAGAA GTCAGGACAG AGAAGCTCTG TCTCAAGAAG TTATGTCTGT GATTGCCCAG
    GTGAAGGAAC ATTTACAAGT TCAAAGCTTC TCAAATGTAG ATGACAGATC TTATGTTCCC
    GAGAGTTTCG AGTGCAGGTT GGATAAACTT ATCTTGCATG GGCAAGGGGA TTTCCAAAAA
    ATCCTTGACA CCTATTCACA GCACCATACT TCGGTGTGTG AAGTATTTGA AAGTGATTGT
    GGAAACAACA AAAATGAACA GAAAGATGCA AAAACAGGAG TCTGCATCAC TGCTCTAAAA
    ACAGAAATAA AAAACATAGA TACTGTGAGT ACTACTCAAG AAAAGCCACA TTGTCAAAGA
    GACACAGGAA TATCTTCCTC CCTAATGGGT AAGAATGTTC AGAGGGAACT CAGAAGGGGA
    GGAAGGAGAA ACTGGACCCA TTCTGATGCA TTTCGAGTCT CCTTGGATCA AGATGTGGAG
    ACTGAGACTG AGCCATCGGA CTACAGCAAT GGTGAGGGAG CTGTTTTCAA CAAGTCTCTG
    AGTGGCAGCC AGACTTCCAG TGCTTGGAGC AACTTATCAG GGTTTAGTTC CTCTGCAAGC
    TGGGAGGAAG TGAATTATCA CGTTGACGAC AGGTCAGCCA GAAAAGAGCC TGGCAAAGAA
    CATCTGGTGG ACACTCAGTG TTCCACTGCC TTGTCTGAGG AGCTAGAGAA TGACAGGGAA
    GGCAGAGCTA TGCATTCATT GCATTCACAG CTTCATGATC TCTCTCTTCA GGAACCCAAC
    AATGACAATT TGGAGCCTTC TCAAAATCAG CCACAGCAAC AGATGCCCTT GACACCCTTC
    TCGCCTCATA ATACCCCAGG CATTTTCTTG GCCCCTGGTG CAGGGCTTCT AGAAGGAGCT
    CCAGAAGGTA TCCAGGAAGT CAGAAATATG GGACCCAGAA ATACTTCTGC TCACTCCAGA
    CCCTCATATC GTTCTGCTTC TTGGTCTTCT GATTCTGGTA GGCCCAAGAA TATGGGCACA
    CATCCTTCAG TCCAAAAAGA AGAAGCCTTT GAAATAATTG TTGAGTTTCC AGAAACCAAC
    TGCGATGTCA AAGACAGGCA GGGGAAAGAG CAGGGAGAAG AAATTAGTGA AAGAGGCGCA
    GGCCCTACAT TTAAAGCTAG TCCCTCCTGG GTTGACCCAG AAGGAGAAAC AGCAGAAAGC
    ACTGAAGATG CACCCTTAGA CTTTCACAGG GTCCTGCACA ATTCTCTGGG AAACATTTCC
    ATGCTGCCAT GTAGCTCCTT CACCCCTAAT TGGCCTGTTC AAAATCCTGA CTCCAGAAAA
    AGTGGTGGCC CAGTCGCAGA GCAGGGCATC GACCCTGATG CCTCCACAGT GGATGAGGAG
    GGGCAACTGC TCGACAGCAT GGATGTTCCC TGCACAAATG GGCACGGCTC TCATAGACTG
    TGCATTCTGA GACAGCCGCC TGGTCAGAGG GCGGAGACCC CCAATTCCTC TGTAAGCGGT
    AACATCCTCT TCCCTGTCCT CAGCGAGGAC TGCACTACCA CAGAGGAAGG AAATCAGCCT
    GGAAACATGC TAAACTGCAG CCAGAACTCC AGCTCATCCT CAGTGTGGTG GCTGAAATCA
    CCTGCATTTT CCAGTGGTTC TTCTGAGGGG GACAGCCCTT GGTCCTATCT GAATTCCAGT
    GGGAGTTCTT GGGTTTCATT GCCGGGAAAG ATGAGGAAAG AGATCCTTGA GGCTCGCACC
    TTGCAACCTG ATGACTTTGA AAAGCTGTTG GCAGGAGTGA GGCATGATTG GCTGTTTCAG
    AGACTAGAGA ATACGGGGGT TTTTAAGCCC AGTCAACTCC ACCGAGCACA TAGTGCTCTT
    TTGTTAAAAT ATTCAAAAAA ATCTGAACTG TGGACGGCCC AGGAAACTAT TGTCTATTTG
    GGGGACTACT TGACTGTGAA GAAAAAAGGC AGACAAAGAA ATGCTTTTTG GGTTCATCAT
    CTTCATCAAG AAGAAATTCT GGGGAGGTAT GTTGGGAAAG ACTATAAGGA GCAGAAGGGG
    CTCTGGCACC ACTTCACTGA TGTGGAGCGA CAGATGACCG CACAGCACTA TGTGACAGAA
    TTTAACAAGA GACTCTATGA ACAAAACATT CCCACCCAGA TATTCTACAT CCCATCCACA
    ATACTACTGA TTTTAGAGGA CAAGACAATA AAGGGATGTA TCAGTGTGGA GCCTTACATA
    CTGGGAGAAT TTGTAAAATT GTCAAATAAC ACGAAAGTGG TGAAAACAGA ATACAAAGCC
    ACAGAATATG GCTTGGCCTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT
    GTGGTCGATT TACAAGGTTG GGTAACCGGT AATGGAAAAG GACTCATCTA CCTCACAGAT
    CCCCAGATTC ACTCCGTTGA TCAGAAAGTT TTCACTACCA ATTTTGGAAA GAGAGGAATT
    TTTTACTTCT TTAATAACCA GCATGTGGAA TGTAATGAAA TCTGCCATCG TCTTTCTTTG
    ACTAGACCTT CAATGGAGAA ACCATGTAAG TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864128.1
    CDS304..4059
    Translation

    Target ORF information:

    RefSeq Version XM_017008639.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008639.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGCCTCC TGTTTTCTCA CAGAGCCTGC TGAAGGAGTT TGACCACCAT
    TTGCTGTCCG CTGCAGAAGC CTGCAAGCTG GCAGCTGCCT TCAGTGCCTA TACGCCGCTC
    TTCGTGCTCA CAGCTGTGAA TATCCGTGGC ACGTGTTTAT TGTCCTACAG TAGTTCAAAT
    GACTGTCCTC CAGAATTGAA AAACTTACAT CTGTGTGAAG CCAAAGAGGC CTTTGAGATT
    GGCCTCCTCA CCAAGAGAGA TGATGAGCCT GTTACTGGAA AACAGGAGCT TCACAGCTTT
    GTCAAAGCTG CTTTCGGTCT CACCACAGTG CACAGAAGGC TCCATGGGGA GACAGGGACG
    GTCCATGCAG CAAGTCAGCT CTGTAAGGAA GCAATGGGGA AGCTGTACAA TTTCAGCACT
    TCCTCCAGAA GTCAGGACAG AGAAGCTCTG TCTCAAGAAG TTATGTCTGT GATTGCCCAG
    GTGAAGGAAC ATTTACAAGT TCAAAGCTTC TCAAATGTAG ATGACAGATC TTATGTTCCC
    GAGAGTTTCG AGTGCAGGTT GGATAAACTT ATCTTGCATG GGCAAGGGGA TTTCCAAAAA
    ATCCTTGACA CCTATTCACA GCACCATACT TCGGTGTGTG AAGTATTTGA AAGTGATTGT
    GGAAACAACA AAAATGAACA GAAAGATGCA AAAACAGGAG TCTGCATCAC TGCTCTAAAA
    ACAGAAATAA AAAACATAGA TACTGTGAGT ACTACTCAAG AAAAGCCACA TTGTCAAAGA
    GACACAGGAA TATCTTCCTC CCTAATGGGT AAGAATGTTC AGAGGGAACT CAGAAGGGGA
    GGAAGGAGAA ACTGGACCCA TTCTGATGCA TTTCGAGTCT CCTTGGATCA AGATGTGGAG
    ACTGAGACTG AGCCATCGGA CTACAGCAAT GGTGAGGGAG CTGTTTTCAA CAAGTCTCTG
    AGTGGCAGCC AGACTTCCAG TGCTTGGAGC AACTTATCAG GGTTTAGTTC CTCTGCAAGC
    TGGGAGGAAG TGAATTATCA CGTTGACGAC AGGTCAGCCA GAAAAGAGCC TGGCAAAGAA
    CATCTGGTGG ACACTCAGTG TTCCACTGCC TTGTCTGAGG AGCTAGAGAA TGACAGGGAA
    GGCAGAGCTA TGCATTCATT GCATTCACAG CTTCATGATC TCTCTCTTCA GGAACCCAAC
    AATGACAATT TGGAGCCTTC TCAAAATCAG CCACAGCAAC AGATGCCCTT GACACCCTTC
    TCGCCTCATA ATACCCCAGG CATTTTCTTG GCCCCTGGTG CAGGGCTTCT AGAAGGAGCT
    CCAGAAGGTA TCCAGGAAGT CAGAAATATG GGACCCAGAA ATACTTCTGC TCACTCCAGA
    CCCTCATATC GTTCTGCTTC TTGGTCTTCT GATTCTGGTA GGCCCAAGAA TATGGGCACA
    CATCCTTCAG TCCAAAAAGA AGAAGCCTTT GAAATAATTG TTGAGTTTCC AGAAACCAAC
    TGCGATGTCA AAGACAGGCA GGGGAAAGAG CAGGGAGAAG AAATTAGTGA AAGAGGCGCA
    GGCCCTACAT TTAAAGCTAG TCCCTCCTGG GTTGACCCAG AAGGAGAAAC AGCAGAAAGC
    ACTGAAGATG CACCCTTAGA CTTTCACAGG GTCCTGCACA ATTCTCTGGG AAACATTTCC
    ATGCTGCCAT GTAGCTCCTT CACCCCTAAT TGGCCTGTTC AAAATCCTGA CTCCAGAAAA
    AGTGGTGGCC CAGTCGCAGA GCAGGGCATC GACCCTGATG CCTCCACAGT GGATGAGGAG
    GGGCAACTGC TCGACAGCAT GGATGTTCCC TGCACAAATG GGCACGGCTC TCATAGACTG
    TGCATTCTGA GACAGCCGCC TGGTCAGAGG GCGGAGACCC CCAATTCCTC TGTAAGCGGT
    AACATCCTCT TCCCTGTCCT CAGCGAGGAC TGCACTACCA CAGAGGAAGG AAATCAGCCT
    GGAAACATGC TAAACTGCAG CCAGAACTCC AGCTCATCCT CAGTGTGGTG GCTGAAATCA
    CCTGCATTTT CCAGTGGTTC TTCTGAGGGG GACAGCCCTT GGTCCTATCT GAATTCCAGT
    GGGAGTTCTT GGGTTTCATT GCCGGGAAAG ATGAGGAAAG AGATCCTTGA GGCTCGCACC
    TTGCAACCTG ATGACTTTGA AAAGCTGTTG GCAGGAGTGA GGCATGATTG GCTGTTTCAG
    AGACTAGAGA ATACGGGGGT TTTTAAGCCC AGTCAACTCC ACCGAGCACA TAGTGCTCTT
    TTGTTAAAAT ATTCAAAAAA ATCTGAACTG TGGACGGCCC AGGAAACTAT TGTCTATTTG
    GGGGACTACT TGACTGTGAA GAAAAAAGGC AGACAAAGAA ATGCTTTTTG GGTTCATCAT
    CTTCATCAAG AAGAAATTCT GGGGAGGTAT GTTGGGAAAG ACTATAAGGA GCAGAAGGGG
    CTCTGGCACC ACTTCACTGA TGTGGAGCGA CAGATGACCG CACAGCACTA TGTGACAGAA
    TTTAACAAGA GACTCTATGA ACAAAACATT CCCACCCAGA TATTCTACAT CCCATCCACA
    ATACTACTGA TTTTAGAGGA CAAGACAATA AAGGGATGTA TCAGTGTGGA GCCTTACATA
    CTGGGAGAAT TTGTAAAATT GTCAAATAAC ACGAAAGTGG TGAAAACAGA ATACAAAGCC
    ACAGAATATG GCTTGGCCTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT
    GTGGTCGATT TACAAGGTTG GGTAACCGGT AATGGAAAAG GACTCATCTA CCTCACAGAT
    CCCCAGATTC ACTCCGTTGA TCAGAAAGTT TTCACTACCA ATTTTGGAAA GAGAGGAATT
    TTTTACTTCT TTAATAACCA GCATGTGGAA TGTAATGAAA TCTGCCATCG TCTTTCTTTG
    ACTAGACCTT CAATGGAGAA ACCATGTAAG TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95411
    Clone ID Related Accession (Same CDS sequence) XM_017008638.2 , XM_017008636.1 , XM_017008635.1 , XM_017008640.1 , XM_017008638.1 , XM_017008633.1 , XM_017008634.1 , XM_017008637.1 , XM_017008639.1 , XM_017008641.2 , XM_017008641.1
    Accession Version XM_017008641.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3756bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017008641.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGCCTCC TGTTTTCTCA CAGAGCCTGC TGAAGGAGTT TGACCACCAT
    TTGCTGTCCG CTGCAGAAGC CTGCAAGCTG GCAGCTGCCT TCAGTGCCTA TACGCCGCTC
    TTCGTGCTCA CAGCTGTGAA TATCCGTGGC ACGTGTTTAT TGTCCTACAG TAGTTCAAAT
    GACTGTCCTC CAGAATTGAA AAACTTACAT CTGTGTGAAG CCAAAGAGGC CTTTGAGATT
    GGCCTCCTCA CCAAGAGAGA TGATGAGCCT GTTACTGGAA AACAGGAGCT TCACAGCTTT
    GTCAAAGCTG CTTTCGGTCT CACCACAGTG CACAGAAGGC TCCATGGGGA GACAGGGACG
    GTCCATGCAG CAAGTCAGCT CTGTAAGGAA GCAATGGGGA AGCTGTACAA TTTCAGCACT
    TCCTCCAGAA GTCAGGACAG AGAAGCTCTG TCTCAAGAAG TTATGTCTGT GATTGCCCAG
    GTGAAGGAAC ATTTACAAGT TCAAAGCTTC TCAAATGTAG ATGACAGATC TTATGTTCCC
    GAGAGTTTCG AGTGCAGGTT GGATAAACTT ATCTTGCATG GGCAAGGGGA TTTCCAAAAA
    ATCCTTGACA CCTATTCACA GCACCATACT TCGGTGTGTG AAGTATTTGA AAGTGATTGT
    GGAAACAACA AAAATGAACA GAAAGATGCA AAAACAGGAG TCTGCATCAC TGCTCTAAAA
    ACAGAAATAA AAAACATAGA TACTGTGAGT ACTACTCAAG AAAAGCCACA TTGTCAAAGA
    GACACAGGAA TATCTTCCTC CCTAATGGGT AAGAATGTTC AGAGGGAACT CAGAAGGGGA
    GGAAGGAGAA ACTGGACCCA TTCTGATGCA TTTCGAGTCT CCTTGGATCA AGATGTGGAG
    ACTGAGACTG AGCCATCGGA CTACAGCAAT GGTGAGGGAG CTGTTTTCAA CAAGTCTCTG
    AGTGGCAGCC AGACTTCCAG TGCTTGGAGC AACTTATCAG GGTTTAGTTC CTCTGCAAGC
    TGGGAGGAAG TGAATTATCA CGTTGACGAC AGGTCAGCCA GAAAAGAGCC TGGCAAAGAA
    CATCTGGTGG ACACTCAGTG TTCCACTGCC TTGTCTGAGG AGCTAGAGAA TGACAGGGAA
    GGCAGAGCTA TGCATTCATT GCATTCACAG CTTCATGATC TCTCTCTTCA GGAACCCAAC
    AATGACAATT TGGAGCCTTC TCAAAATCAG CCACAGCAAC AGATGCCCTT GACACCCTTC
    TCGCCTCATA ATACCCCAGG CATTTTCTTG GCCCCTGGTG CAGGGCTTCT AGAAGGAGCT
    CCAGAAGGTA TCCAGGAAGT CAGAAATATG GGACCCAGAA ATACTTCTGC TCACTCCAGA
    CCCTCATATC GTTCTGCTTC TTGGTCTTCT GATTCTGGTA GGCCCAAGAA TATGGGCACA
    CATCCTTCAG TCCAAAAAGA AGAAGCCTTT GAAATAATTG TTGAGTTTCC AGAAACCAAC
    TGCGATGTCA AAGACAGGCA GGGGAAAGAG CAGGGAGAAG AAATTAGTGA AAGAGGCGCA
    GGCCCTACAT TTAAAGCTAG TCCCTCCTGG GTTGACCCAG AAGGAGAAAC AGCAGAAAGC
    ACTGAAGATG CACCCTTAGA CTTTCACAGG GTCCTGCACA ATTCTCTGGG AAACATTTCC
    ATGCTGCCAT GTAGCTCCTT CACCCCTAAT TGGCCTGTTC AAAATCCTGA CTCCAGAAAA
    AGTGGTGGCC CAGTCGCAGA GCAGGGCATC GACCCTGATG CCTCCACAGT GGATGAGGAG
    GGGCAACTGC TCGACAGCAT GGATGTTCCC TGCACAAATG GGCACGGCTC TCATAGACTG
    TGCATTCTGA GACAGCCGCC TGGTCAGAGG GCGGAGACCC CCAATTCCTC TGTAAGCGGT
    AACATCCTCT TCCCTGTCCT CAGCGAGGAC TGCACTACCA CAGAGGAAGG AAATCAGCCT
    GGAAACATGC TAAACTGCAG CCAGAACTCC AGCTCATCCT CAGTGTGGTG GCTGAAATCA
    CCTGCATTTT CCAGTGGTTC TTCTGAGGGG GACAGCCCTT GGTCCTATCT GAATTCCAGT
    GGGAGTTCTT GGGTTTCATT GCCGGGAAAG ATGAGGAAAG AGATCCTTGA GGCTCGCACC
    TTGCAACCTG ATGACTTTGA AAAGCTGTTG GCAGGAGTGA GGCATGATTG GCTGTTTCAG
    AGACTAGAGA ATACGGGGGT TTTTAAGCCC AGTCAACTCC ACCGAGCACA TAGTGCTCTT
    TTGTTAAAAT ATTCAAAAAA ATCTGAACTG TGGACGGCCC AGGAAACTAT TGTCTATTTG
    GGGGACTACT TGACTGTGAA GAAAAAAGGC AGACAAAGAA ATGCTTTTTG GGTTCATCAT
    CTTCATCAAG AAGAAATTCT GGGGAGGTAT GTTGGGAAAG ACTATAAGGA GCAGAAGGGG
    CTCTGGCACC ACTTCACTGA TGTGGAGCGA CAGATGACCG CACAGCACTA TGTGACAGAA
    TTTAACAAGA GACTCTATGA ACAAAACATT CCCACCCAGA TATTCTACAT CCCATCCACA
    ATACTACTGA TTTTAGAGGA CAAGACAATA AAGGGATGTA TCAGTGTGGA GCCTTACATA
    CTGGGAGAAT TTGTAAAATT GTCAAATAAC ACGAAAGTGG TGAAAACAGA ATACAAAGCC
    ACAGAATATG GCTTGGCCTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT
    GTGGTCGATT TACAAGGTTG GGTAACCGGT AATGGAAAAG GACTCATCTA CCTCACAGAT
    CCCCAGATTC ACTCCGTTGA TCAGAAAGTT TTCACTACCA ATTTTGGAAA GAGAGGAATT
    TTTTACTTCT TTAATAACCA GCATGTGGAA TGTAATGAAA TCTGCCATCG TCTTTCTTTG
    ACTAGACCTT CAATGGAGAA ACCATGTAAG TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864130.1
    CDS742..4497
    Translation

    Target ORF information:

    RefSeq Version XM_017008641.2
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008641.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGCCTCC TGTTTTCTCA CAGAGCCTGC TGAAGGAGTT TGACCACCAT
    TTGCTGTCCG CTGCAGAAGC CTGCAAGCTG GCAGCTGCCT TCAGTGCCTA TACGCCGCTC
    TTCGTGCTCA CAGCTGTGAA TATCCGTGGC ACGTGTTTAT TGTCCTACAG TAGTTCAAAT
    GACTGTCCTC CAGAATTGAA AAACTTACAT CTGTGTGAAG CCAAAGAGGC CTTTGAGATT
    GGCCTCCTCA CCAAGAGAGA TGATGAGCCT GTTACTGGAA AACAGGAGCT TCACAGCTTT
    GTCAAAGCTG CTTTCGGTCT CACCACAGTG CACAGAAGGC TCCATGGGGA GACAGGGACG
    GTCCATGCAG CAAGTCAGCT CTGTAAGGAA GCAATGGGGA AGCTGTACAA TTTCAGCACT
    TCCTCCAGAA GTCAGGACAG AGAAGCTCTG TCTCAAGAAG TTATGTCTGT GATTGCCCAG
    GTGAAGGAAC ATTTACAAGT TCAAAGCTTC TCAAATGTAG ATGACAGATC TTATGTTCCC
    GAGAGTTTCG AGTGCAGGTT GGATAAACTT ATCTTGCATG GGCAAGGGGA TTTCCAAAAA
    ATCCTTGACA CCTATTCACA GCACCATACT TCGGTGTGTG AAGTATTTGA AAGTGATTGT
    GGAAACAACA AAAATGAACA GAAAGATGCA AAAACAGGAG TCTGCATCAC TGCTCTAAAA
    ACAGAAATAA AAAACATAGA TACTGTGAGT ACTACTCAAG AAAAGCCACA TTGTCAAAGA
    GACACAGGAA TATCTTCCTC CCTAATGGGT AAGAATGTTC AGAGGGAACT CAGAAGGGGA
    GGAAGGAGAA ACTGGACCCA TTCTGATGCA TTTCGAGTCT CCTTGGATCA AGATGTGGAG
    ACTGAGACTG AGCCATCGGA CTACAGCAAT GGTGAGGGAG CTGTTTTCAA CAAGTCTCTG
    AGTGGCAGCC AGACTTCCAG TGCTTGGAGC AACTTATCAG GGTTTAGTTC CTCTGCAAGC
    TGGGAGGAAG TGAATTATCA CGTTGACGAC AGGTCAGCCA GAAAAGAGCC TGGCAAAGAA
    CATCTGGTGG ACACTCAGTG TTCCACTGCC TTGTCTGAGG AGCTAGAGAA TGACAGGGAA
    GGCAGAGCTA TGCATTCATT GCATTCACAG CTTCATGATC TCTCTCTTCA GGAACCCAAC
    AATGACAATT TGGAGCCTTC TCAAAATCAG CCACAGCAAC AGATGCCCTT GACACCCTTC
    TCGCCTCATA ATACCCCAGG CATTTTCTTG GCCCCTGGTG CAGGGCTTCT AGAAGGAGCT
    CCAGAAGGTA TCCAGGAAGT CAGAAATATG GGACCCAGAA ATACTTCTGC TCACTCCAGA
    CCCTCATATC GTTCTGCTTC TTGGTCTTCT GATTCTGGTA GGCCCAAGAA TATGGGCACA
    CATCCTTCAG TCCAAAAAGA AGAAGCCTTT GAAATAATTG TTGAGTTTCC AGAAACCAAC
    TGCGATGTCA AAGACAGGCA GGGGAAAGAG CAGGGAGAAG AAATTAGTGA AAGAGGCGCA
    GGCCCTACAT TTAAAGCTAG TCCCTCCTGG GTTGACCCAG AAGGAGAAAC AGCAGAAAGC
    ACTGAAGATG CACCCTTAGA CTTTCACAGG GTCCTGCACA ATTCTCTGGG AAACATTTCC
    ATGCTGCCAT GTAGCTCCTT CACCCCTAAT TGGCCTGTTC AAAATCCTGA CTCCAGAAAA
    AGTGGTGGCC CAGTCGCAGA GCAGGGCATC GACCCTGATG CCTCCACAGT GGATGAGGAG
    GGGCAACTGC TCGACAGCAT GGATGTTCCC TGCACAAATG GGCACGGCTC TCATAGACTG
    TGCATTCTGA GACAGCCGCC TGGTCAGAGG GCGGAGACCC CCAATTCCTC TGTAAGCGGT
    AACATCCTCT TCCCTGTCCT CAGCGAGGAC TGCACTACCA CAGAGGAAGG AAATCAGCCT
    GGAAACATGC TAAACTGCAG CCAGAACTCC AGCTCATCCT CAGTGTGGTG GCTGAAATCA
    CCTGCATTTT CCAGTGGTTC TTCTGAGGGG GACAGCCCTT GGTCCTATCT GAATTCCAGT
    GGGAGTTCTT GGGTTTCATT GCCGGGAAAG ATGAGGAAAG AGATCCTTGA GGCTCGCACC
    TTGCAACCTG ATGACTTTGA AAAGCTGTTG GCAGGAGTGA GGCATGATTG GCTGTTTCAG
    AGACTAGAGA ATACGGGGGT TTTTAAGCCC AGTCAACTCC ACCGAGCACA TAGTGCTCTT
    TTGTTAAAAT ATTCAAAAAA ATCTGAACTG TGGACGGCCC AGGAAACTAT TGTCTATTTG
    GGGGACTACT TGACTGTGAA GAAAAAAGGC AGACAAAGAA ATGCTTTTTG GGTTCATCAT
    CTTCATCAAG AAGAAATTCT GGGGAGGTAT GTTGGGAAAG ACTATAAGGA GCAGAAGGGG
    CTCTGGCACC ACTTCACTGA TGTGGAGCGA CAGATGACCG CACAGCACTA TGTGACAGAA
    TTTAACAAGA GACTCTATGA ACAAAACATT CCCACCCAGA TATTCTACAT CCCATCCACA
    ATACTACTGA TTTTAGAGGA CAAGACAATA AAGGGATGTA TCAGTGTGGA GCCTTACATA
    CTGGGAGAAT TTGTAAAATT GTCAAATAAC ACGAAAGTGG TGAAAACAGA ATACAAAGCC
    ACAGAATATG GCTTGGCCTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT
    GTGGTCGATT TACAAGGTTG GGTAACCGGT AATGGAAAAG GACTCATCTA CCTCACAGAT
    CCCCAGATTC ACTCCGTTGA TCAGAAAGTT TTCACTACCA ATTTTGGAAA GAGAGGAATT
    TTTTACTTCT TTAATAACCA GCATGTGGAA TGTAATGAAA TCTGCCATCG TCTTTCTTTG
    ACTAGACCTT CAATGGAGAA ACCATGTAAG TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95411
    Clone ID Related Accession (Same CDS sequence) XM_017008638.2 , XM_017008636.1 , XM_017008635.1 , XM_017008640.1 , XM_017008638.1 , XM_017008633.1 , XM_017008634.1 , XM_017008637.1 , XM_017008639.1 , XM_017008641.2 , XM_017008641.1
    Accession Version XM_017008641.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3756bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGCCTCC TGTTTTCTCA CAGAGCCTGC TGAAGGAGTT TGACCACCAT
    TTGCTGTCCG CTGCAGAAGC CTGCAAGCTG GCAGCTGCCT TCAGTGCCTA TACGCCGCTC
    TTCGTGCTCA CAGCTGTGAA TATCCGTGGC ACGTGTTTAT TGTCCTACAG TAGTTCAAAT
    GACTGTCCTC CAGAATTGAA AAACTTACAT CTGTGTGAAG CCAAAGAGGC CTTTGAGATT
    GGCCTCCTCA CCAAGAGAGA TGATGAGCCT GTTACTGGAA AACAGGAGCT TCACAGCTTT
    GTCAAAGCTG CTTTCGGTCT CACCACAGTG CACAGAAGGC TCCATGGGGA GACAGGGACG
    GTCCATGCAG CAAGTCAGCT CTGTAAGGAA GCAATGGGGA AGCTGTACAA TTTCAGCACT
    TCCTCCAGAA GTCAGGACAG AGAAGCTCTG TCTCAAGAAG TTATGTCTGT GATTGCCCAG
    GTGAAGGAAC ATTTACAAGT TCAAAGCTTC TCAAATGTAG ATGACAGATC TTATGTTCCC
    GAGAGTTTCG AGTGCAGGTT GGATAAACTT ATCTTGCATG GGCAAGGGGA TTTCCAAAAA
    ATCCTTGACA CCTATTCACA GCACCATACT TCGGTGTGTG AAGTATTTGA AAGTGATTGT
    GGAAACAACA AAAATGAACA GAAAGATGCA AAAACAGGAG TCTGCATCAC TGCTCTAAAA
    ACAGAAATAA AAAACATAGA TACTGTGAGT ACTACTCAAG AAAAGCCACA TTGTCAAAGA
    GACACAGGAA TATCTTCCTC CCTAATGGGT AAGAATGTTC AGAGGGAACT CAGAAGGGGA
    GGAAGGAGAA ACTGGACCCA TTCTGATGCA TTTCGAGTCT CCTTGGATCA AGATGTGGAG
    ACTGAGACTG AGCCATCGGA CTACAGCAAT GGTGAGGGAG CTGTTTTCAA CAAGTCTCTG
    AGTGGCAGCC AGACTTCCAG TGCTTGGAGC AACTTATCAG GGTTTAGTTC CTCTGCAAGC
    TGGGAGGAAG TGAATTATCA CGTTGACGAC AGGTCAGCCA GAAAAGAGCC TGGCAAAGAA
    CATCTGGTGG ACACTCAGTG TTCCACTGCC TTGTCTGAGG AGCTAGAGAA TGACAGGGAA
    GGCAGAGCTA TGCATTCATT GCATTCACAG CTTCATGATC TCTCTCTTCA GGAACCCAAC
    AATGACAATT TGGAGCCTTC TCAAAATCAG CCACAGCAAC AGATGCCCTT GACACCCTTC
    TCGCCTCATA ATACCCCAGG CATTTTCTTG GCCCCTGGTG CAGGGCTTCT AGAAGGAGCT
    CCAGAAGGTA TCCAGGAAGT CAGAAATATG GGACCCAGAA ATACTTCTGC TCACTCCAGA
    CCCTCATATC GTTCTGCTTC TTGGTCTTCT GATTCTGGTA GGCCCAAGAA TATGGGCACA
    CATCCTTCAG TCCAAAAAGA AGAAGCCTTT GAAATAATTG TTGAGTTTCC AGAAACCAAC
    TGCGATGTCA AAGACAGGCA GGGGAAAGAG CAGGGAGAAG AAATTAGTGA AAGAGGCGCA
    GGCCCTACAT TTAAAGCTAG TCCCTCCTGG GTTGACCCAG AAGGAGAAAC AGCAGAAAGC
    ACTGAAGATG CACCCTTAGA CTTTCACAGG GTCCTGCACA ATTCTCTGGG AAACATTTCC
    ATGCTGCCAT GTAGCTCCTT CACCCCTAAT TGGCCTGTTC AAAATCCTGA CTCCAGAAAA
    AGTGGTGGCC CAGTCGCAGA GCAGGGCATC GACCCTGATG CCTCCACAGT GGATGAGGAG
    GGGCAACTGC TCGACAGCAT GGATGTTCCC TGCACAAATG GGCACGGCTC TCATAGACTG
    TGCATTCTGA GACAGCCGCC TGGTCAGAGG GCGGAGACCC CCAATTCCTC TGTAAGCGGT
    AACATCCTCT TCCCTGTCCT CAGCGAGGAC TGCACTACCA CAGAGGAAGG AAATCAGCCT
    GGAAACATGC TAAACTGCAG CCAGAACTCC AGCTCATCCT CAGTGTGGTG GCTGAAATCA
    CCTGCATTTT CCAGTGGTTC TTCTGAGGGG GACAGCCCTT GGTCCTATCT GAATTCCAGT
    GGGAGTTCTT GGGTTTCATT GCCGGGAAAG ATGAGGAAAG AGATCCTTGA GGCTCGCACC
    TTGCAACCTG ATGACTTTGA AAAGCTGTTG GCAGGAGTGA GGCATGATTG GCTGTTTCAG
    AGACTAGAGA ATACGGGGGT TTTTAAGCCC AGTCAACTCC ACCGAGCACA TAGTGCTCTT
    TTGTTAAAAT ATTCAAAAAA ATCTGAACTG TGGACGGCCC AGGAAACTAT TGTCTATTTG
    GGGGACTACT TGACTGTGAA GAAAAAAGGC AGACAAAGAA ATGCTTTTTG GGTTCATCAT
    CTTCATCAAG AAGAAATTCT GGGGAGGTAT GTTGGGAAAG ACTATAAGGA GCAGAAGGGG
    CTCTGGCACC ACTTCACTGA TGTGGAGCGA CAGATGACCG CACAGCACTA TGTGACAGAA
    TTTAACAAGA GACTCTATGA ACAAAACATT CCCACCCAGA TATTCTACAT CCCATCCACA
    ATACTACTGA TTTTAGAGGA CAAGACAATA AAGGGATGTA TCAGTGTGGA GCCTTACATA
    CTGGGAGAAT TTGTAAAATT GTCAAATAAC ACGAAAGTGG TGAAAACAGA ATACAAAGCC
    ACAGAATATG GCTTGGCCTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT
    GTGGTCGATT TACAAGGTTG GGTAACCGGT AATGGAAAAG GACTCATCTA CCTCACAGAT
    CCCCAGATTC ACTCCGTTGA TCAGAAAGTT TTCACTACCA ATTTTGGAAA GAGAGGAATT
    TTTTACTTCT TTAATAACCA GCATGTGGAA TGTAATGAAA TCTGCCATCG TCTTTCTTTG
    ACTAGACCTT CAATGGAGAA ACCATGTAAG TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864130.1
    CDS317..4072
    Translation

    Target ORF information:

    RefSeq Version XM_017008641.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008641.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGCCTCC TGTTTTCTCA CAGAGCCTGC TGAAGGAGTT TGACCACCAT
    TTGCTGTCCG CTGCAGAAGC CTGCAAGCTG GCAGCTGCCT TCAGTGCCTA TACGCCGCTC
    TTCGTGCTCA CAGCTGTGAA TATCCGTGGC ACGTGTTTAT TGTCCTACAG TAGTTCAAAT
    GACTGTCCTC CAGAATTGAA AAACTTACAT CTGTGTGAAG CCAAAGAGGC CTTTGAGATT
    GGCCTCCTCA CCAAGAGAGA TGATGAGCCT GTTACTGGAA AACAGGAGCT TCACAGCTTT
    GTCAAAGCTG CTTTCGGTCT CACCACAGTG CACAGAAGGC TCCATGGGGA GACAGGGACG
    GTCCATGCAG CAAGTCAGCT CTGTAAGGAA GCAATGGGGA AGCTGTACAA TTTCAGCACT
    TCCTCCAGAA GTCAGGACAG AGAAGCTCTG TCTCAAGAAG TTATGTCTGT GATTGCCCAG
    GTGAAGGAAC ATTTACAAGT TCAAAGCTTC TCAAATGTAG ATGACAGATC TTATGTTCCC
    GAGAGTTTCG AGTGCAGGTT GGATAAACTT ATCTTGCATG GGCAAGGGGA TTTCCAAAAA
    ATCCTTGACA CCTATTCACA GCACCATACT TCGGTGTGTG AAGTATTTGA AAGTGATTGT
    GGAAACAACA AAAATGAACA GAAAGATGCA AAAACAGGAG TCTGCATCAC TGCTCTAAAA
    ACAGAAATAA AAAACATAGA TACTGTGAGT ACTACTCAAG AAAAGCCACA TTGTCAAAGA
    GACACAGGAA TATCTTCCTC CCTAATGGGT AAGAATGTTC AGAGGGAACT CAGAAGGGGA
    GGAAGGAGAA ACTGGACCCA TTCTGATGCA TTTCGAGTCT CCTTGGATCA AGATGTGGAG
    ACTGAGACTG AGCCATCGGA CTACAGCAAT GGTGAGGGAG CTGTTTTCAA CAAGTCTCTG
    AGTGGCAGCC AGACTTCCAG TGCTTGGAGC AACTTATCAG GGTTTAGTTC CTCTGCAAGC
    TGGGAGGAAG TGAATTATCA CGTTGACGAC AGGTCAGCCA GAAAAGAGCC TGGCAAAGAA
    CATCTGGTGG ACACTCAGTG TTCCACTGCC TTGTCTGAGG AGCTAGAGAA TGACAGGGAA
    GGCAGAGCTA TGCATTCATT GCATTCACAG CTTCATGATC TCTCTCTTCA GGAACCCAAC
    AATGACAATT TGGAGCCTTC TCAAAATCAG CCACAGCAAC AGATGCCCTT GACACCCTTC
    TCGCCTCATA ATACCCCAGG CATTTTCTTG GCCCCTGGTG CAGGGCTTCT AGAAGGAGCT
    CCAGAAGGTA TCCAGGAAGT CAGAAATATG GGACCCAGAA ATACTTCTGC TCACTCCAGA
    CCCTCATATC GTTCTGCTTC TTGGTCTTCT GATTCTGGTA GGCCCAAGAA TATGGGCACA
    CATCCTTCAG TCCAAAAAGA AGAAGCCTTT GAAATAATTG TTGAGTTTCC AGAAACCAAC
    TGCGATGTCA AAGACAGGCA GGGGAAAGAG CAGGGAGAAG AAATTAGTGA AAGAGGCGCA
    GGCCCTACAT TTAAAGCTAG TCCCTCCTGG GTTGACCCAG AAGGAGAAAC AGCAGAAAGC
    ACTGAAGATG CACCCTTAGA CTTTCACAGG GTCCTGCACA ATTCTCTGGG AAACATTTCC
    ATGCTGCCAT GTAGCTCCTT CACCCCTAAT TGGCCTGTTC AAAATCCTGA CTCCAGAAAA
    AGTGGTGGCC CAGTCGCAGA GCAGGGCATC GACCCTGATG CCTCCACAGT GGATGAGGAG
    GGGCAACTGC TCGACAGCAT GGATGTTCCC TGCACAAATG GGCACGGCTC TCATAGACTG
    TGCATTCTGA GACAGCCGCC TGGTCAGAGG GCGGAGACCC CCAATTCCTC TGTAAGCGGT
    AACATCCTCT TCCCTGTCCT CAGCGAGGAC TGCACTACCA CAGAGGAAGG AAATCAGCCT
    GGAAACATGC TAAACTGCAG CCAGAACTCC AGCTCATCCT CAGTGTGGTG GCTGAAATCA
    CCTGCATTTT CCAGTGGTTC TTCTGAGGGG GACAGCCCTT GGTCCTATCT GAATTCCAGT
    GGGAGTTCTT GGGTTTCATT GCCGGGAAAG ATGAGGAAAG AGATCCTTGA GGCTCGCACC
    TTGCAACCTG ATGACTTTGA AAAGCTGTTG GCAGGAGTGA GGCATGATTG GCTGTTTCAG
    AGACTAGAGA ATACGGGGGT TTTTAAGCCC AGTCAACTCC ACCGAGCACA TAGTGCTCTT
    TTGTTAAAAT ATTCAAAAAA ATCTGAACTG TGGACGGCCC AGGAAACTAT TGTCTATTTG
    GGGGACTACT TGACTGTGAA GAAAAAAGGC AGACAAAGAA ATGCTTTTTG GGTTCATCAT
    CTTCATCAAG AAGAAATTCT GGGGAGGTAT GTTGGGAAAG ACTATAAGGA GCAGAAGGGG
    CTCTGGCACC ACTTCACTGA TGTGGAGCGA CAGATGACCG CACAGCACTA TGTGACAGAA
    TTTAACAAGA GACTCTATGA ACAAAACATT CCCACCCAGA TATTCTACAT CCCATCCACA
    ATACTACTGA TTTTAGAGGA CAAGACAATA AAGGGATGTA TCAGTGTGGA GCCTTACATA
    CTGGGAGAAT TTGTAAAATT GTCAAATAAC ACGAAAGTGG TGAAAACAGA ATACAAAGCC
    ACAGAATATG GCTTGGCCTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT
    GTGGTCGATT TACAAGGTTG GGTAACCGGT AATGGAAAAG GACTCATCTA CCTCACAGAT
    CCCCAGATTC ACTCCGTTGA TCAGAAAGTT TTCACTACCA ATTTTGGAAA GAGAGGAATT
    TTTTACTTCT TTAATAACCA GCATGTGGAA TGTAATGAAA TCTGCCATCG TCTTTCTTTG
    ACTAGACCTT CAATGGAGAA ACCATGTAAG TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95412
    Clone ID Related Accession (Same CDS sequence) XM_017008642.1
    Accession Version XM_017008642.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3753bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGCCTCC TGTTTTCTCA CAGAGCCTGC TGAAGGAGTT TGACCACCAT
    TTGCTGTCCG CTGCAGAAGC CTGCAAGCTG GCAGCTGCCT TCAGTGCCTA TACGCCGCTC
    TTCGTGCTCA CAGCTGTGAA TATCCGTGGC ACGTGTTTAT TGTCCTACAG TAGTTCAAAT
    GACTGTCCTC CAGAATTGAA AAACTTACAT CTGTGTGAAG CCAAAGAGGC CTTTGAGATT
    GGCCTCCTCA CCAAGAGAGA TGATGAGCCT GTTACTGGAA AACAGGAGCT TCACAGCTTT
    GTCAAAGCTG CTTTCGGTCT CACCACAGTG CACAGAAGGC TCCATGGGGA GACAGGGACG
    GTCCATGCAG CAAGTCAGCT CTGTAAGGAA GCAATGGGGA AGCTGTACAA TTTCAGCACT
    TCCTCCAGAA GTCAGGACAG AGAAGCTCTG TCTCAAGAAG TTATGTCTGT GATTGCCCAG
    GTGAAGGAAC ATTTACAAGT TCAAAGCTTC TCAAATGTAG ATGACAGATC TTATGTTCCC
    GAGAGTTTCG AGTGCAGGTT GGATAAACTT ATCTTGCATG GGCAAGGGGA TTTCCAAAAA
    ATCCTTGACA CCTATTCACA GCACCATACT TCGGTGTGTG AAGTATTTGA AAGTGATTGT
    GGAAACAACA AAAATGAACA GAAAGATGCA AAAACAGGAG TCTGCATCAC TGCTCTAAAA
    ACAGAAATAA AAAACATAGA TACTGTGAGT ACTACTCAAG AAAAGCCACA TTGTCAAAGA
    GACACAGGAA TATCTTCCTC CCTAATGGGT AAGAATGTTC AGAGGGAACT CAGAAGGGGA
    GGAAGGAGAA ACTGGACCCA TTCTGATGCA TTTCGAGTCT CCTTGGATCA AGATGTGGAG
    ACTGAGACTG AGCCATCGGA CTACAGCAAT GGTGAGGGAG CTGTTTTCAA CAAGTCTCTG
    AGTGGCAGCC AGACTTCCAG TGCTTGGAGC AACTTATCAG GGTTTAGTTC CTCTGCAAGC
    TGGGAGGAAG TGAATTATCA CGTTGACGAC AGGTCAGCCA GAAAAGAGCC TGGCAAAGAA
    CATCTGGTGG ACACTCAGTG TTCCACTGCC TTGTCTGAGG AGCTAGAGAA TGACAGGGAA
    GGCAGAGCTA TGCATTCATT GCATTCACAG CTTCATGATC TCTCTCTTCA GGAACCCAAC
    AATGACAATT TGGAGCCTTC TCAAAATCAG CCACAGCAAC AGATGCCCTT GACACCCTTC
    TCGCCTCATA ATACCCCAGG CATTTTCTTG GCCCCTGGTG CAGGGCTTCT AGAAGGAGCT
    CCAGAAGGTA TCCAGGAAGT CAGAAATATG GGACCCAGAA ATACTTCTGC TCACTCCAGA
    CCCTCATATC GTTCTGCTTC TTGGTCTTCT GATTCTGGTA GGCCCAAGAA TATGGGCACA
    CATCCTTCAG TCCAAAAAGA AGAAGCCTTT GAAATAATTG TTGAGTTTCC AGAAACCAAC
    TGCGATGTCA AAGACAGGCA GGGGAAAGAG CAGGGAGAAG AAATTAGTGA AAGAGGCGCA
    GGCCCTACAT TTAAAGCTAG TCCCTCCTGG GTTGACCCAG AAGGAGAAAC AGCAGAAAGC
    ACTGAAGATG CACCCTTAGA CTTTCACAGG GTCCTGCACA ATTCTCTGGG AAACATTTCC
    ATGCTGCCAT GTAGCTCCTT CACCCCTAAT TGGCCTGTTC AAAATCCTGA CTCCAGAAAA
    AGTGGTGGCC CAGTCGCAGA GCAGGGCATC GACCCTGATG CCTCCACAGT GGATGAGGAG
    GGGCAACTGC TCGACAGCAT GGATGTTCCC TGCACAAATG GGCACGGCTC TCATAGACTG
    TGCATTCTGA GACAGCCGCC TGGTCAGAGG GCGGAGACCC CCAATTCCTC TGTAAGCGGT
    AACATCCTCT TCCCTGTCCT CAGCGAGGAC TGCACTACCA CAGAGGAAGG AAATCAGCCT
    GGAAACATGC TAAACTGCAG CCAGAACTCC AGCTCATCCT CAGTGTGGTG GCTGAAATCA
    CCTGCATTTT CCAGTGGTTC TTCTGAGGGG GACAGCCCTT GGTCCTATCT GAATTCCAGT
    GGGAGTTCTT GGGTTTCATT GCCGGGAAAG ATGAGGAAAG AGATCCTTGA GGCTCGCACC
    TTGCAACCTG ATGACTTTGA AAAGCTGTTG GCAGGAGTGA GGCATGATTG GCTGTTTCAG
    AGACTAGAGA ATACGGGGGT TTTTAAGCCC AGTCAACTCC ACCGAGCACA TAGTGCTCTT
    TTGTTAAAAT ATTCAAAAAA ATCTGAACTG TGGACGGCCC AGGAAACTAT TGTCTATTTG
    GGGGACTACT TGACTGTGAA GAAAAAAGGC AGACAAAGAA ATGCTTTTTG GGTTCATCAT
    CTTCATCAAG AAGAAATTCT GGGGAGGTAT GTTGGGAAAG ACTATAAGGA GCAGAAGGGG
    CTCTGGCACC ACTTCACTGA TGTGGAGCGA CAGATGACCG CACAGCACTA TGTGACAGAA
    TTTAACAAGA GACTCTATGA ACAAAACATT CCCACCCAGA TATTCTACAT CCCATCCACA
    ATACTACTGA TTTTAGAGGA CAAGACAATA AAGGGATGTA TCAGTGTGGA GCCTTACATA
    CTGGGAGAAT TTGTAAAATT GTCAAATAAC ACGAAAGTGG TGAAAACAGA ATACAAAGCC
    ACAGAATATG GCTTGGCCTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT
    GTGGTCGATT TACAAGGTTG GGTAACCGGT AATGGAAAAG GACTCATCTA CCTCACAGAT
    CCCCAGATTC ACTCCGTTGA TCAGAAAGTT TTCACTACCA ATTTTGGAAA GAGAGGAATT
    TTTTACTTCT TTAATAACCA GCATGTGGAA TGTAATGAAA TCTGCCATCG TCTTTCTTTG
    ACTAGACCTT CAATGGAGAA ACCATGCACA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864131.1
    CDS280..4032
    Translation

    Target ORF information:

    RefSeq Version XM_017008642.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008642.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGCCTCC TGTTTTCTCA CAGAGCCTGC TGAAGGAGTT TGACCACCAT
    TTGCTGTCCG CTGCAGAAGC CTGCAAGCTG GCAGCTGCCT TCAGTGCCTA TACGCCGCTC
    TTCGTGCTCA CAGCTGTGAA TATCCGTGGC ACGTGTTTAT TGTCCTACAG TAGTTCAAAT
    GACTGTCCTC CAGAATTGAA AAACTTACAT CTGTGTGAAG CCAAAGAGGC CTTTGAGATT
    GGCCTCCTCA CCAAGAGAGA TGATGAGCCT GTTACTGGAA AACAGGAGCT TCACAGCTTT
    GTCAAAGCTG CTTTCGGTCT CACCACAGTG CACAGAAGGC TCCATGGGGA GACAGGGACG
    GTCCATGCAG CAAGTCAGCT CTGTAAGGAA GCAATGGGGA AGCTGTACAA TTTCAGCACT
    TCCTCCAGAA GTCAGGACAG AGAAGCTCTG TCTCAAGAAG TTATGTCTGT GATTGCCCAG
    GTGAAGGAAC ATTTACAAGT TCAAAGCTTC TCAAATGTAG ATGACAGATC TTATGTTCCC
    GAGAGTTTCG AGTGCAGGTT GGATAAACTT ATCTTGCATG GGCAAGGGGA TTTCCAAAAA
    ATCCTTGACA CCTATTCACA GCACCATACT TCGGTGTGTG AAGTATTTGA AAGTGATTGT
    GGAAACAACA AAAATGAACA GAAAGATGCA AAAACAGGAG TCTGCATCAC TGCTCTAAAA
    ACAGAAATAA AAAACATAGA TACTGTGAGT ACTACTCAAG AAAAGCCACA TTGTCAAAGA
    GACACAGGAA TATCTTCCTC CCTAATGGGT AAGAATGTTC AGAGGGAACT CAGAAGGGGA
    GGAAGGAGAA ACTGGACCCA TTCTGATGCA TTTCGAGTCT CCTTGGATCA AGATGTGGAG
    ACTGAGACTG AGCCATCGGA CTACAGCAAT GGTGAGGGAG CTGTTTTCAA CAAGTCTCTG
    AGTGGCAGCC AGACTTCCAG TGCTTGGAGC AACTTATCAG GGTTTAGTTC CTCTGCAAGC
    TGGGAGGAAG TGAATTATCA CGTTGACGAC AGGTCAGCCA GAAAAGAGCC TGGCAAAGAA
    CATCTGGTGG ACACTCAGTG TTCCACTGCC TTGTCTGAGG AGCTAGAGAA TGACAGGGAA
    GGCAGAGCTA TGCATTCATT GCATTCACAG CTTCATGATC TCTCTCTTCA GGAACCCAAC
    AATGACAATT TGGAGCCTTC TCAAAATCAG CCACAGCAAC AGATGCCCTT GACACCCTTC
    TCGCCTCATA ATACCCCAGG CATTTTCTTG GCCCCTGGTG CAGGGCTTCT AGAAGGAGCT
    CCAGAAGGTA TCCAGGAAGT CAGAAATATG GGACCCAGAA ATACTTCTGC TCACTCCAGA
    CCCTCATATC GTTCTGCTTC TTGGTCTTCT GATTCTGGTA GGCCCAAGAA TATGGGCACA
    CATCCTTCAG TCCAAAAAGA AGAAGCCTTT GAAATAATTG TTGAGTTTCC AGAAACCAAC
    TGCGATGTCA AAGACAGGCA GGGGAAAGAG CAGGGAGAAG AAATTAGTGA AAGAGGCGCA
    GGCCCTACAT TTAAAGCTAG TCCCTCCTGG GTTGACCCAG AAGGAGAAAC AGCAGAAAGC
    ACTGAAGATG CACCCTTAGA CTTTCACAGG GTCCTGCACA ATTCTCTGGG AAACATTTCC
    ATGCTGCCAT GTAGCTCCTT CACCCCTAAT TGGCCTGTTC AAAATCCTGA CTCCAGAAAA
    AGTGGTGGCC CAGTCGCAGA GCAGGGCATC GACCCTGATG CCTCCACAGT GGATGAGGAG
    GGGCAACTGC TCGACAGCAT GGATGTTCCC TGCACAAATG GGCACGGCTC TCATAGACTG
    TGCATTCTGA GACAGCCGCC TGGTCAGAGG GCGGAGACCC CCAATTCCTC TGTAAGCGGT
    AACATCCTCT TCCCTGTCCT CAGCGAGGAC TGCACTACCA CAGAGGAAGG AAATCAGCCT
    GGAAACATGC TAAACTGCAG CCAGAACTCC AGCTCATCCT CAGTGTGGTG GCTGAAATCA
    CCTGCATTTT CCAGTGGTTC TTCTGAGGGG GACAGCCCTT GGTCCTATCT GAATTCCAGT
    GGGAGTTCTT GGGTTTCATT GCCGGGAAAG ATGAGGAAAG AGATCCTTGA GGCTCGCACC
    TTGCAACCTG ATGACTTTGA AAAGCTGTTG GCAGGAGTGA GGCATGATTG GCTGTTTCAG
    AGACTAGAGA ATACGGGGGT TTTTAAGCCC AGTCAACTCC ACCGAGCACA TAGTGCTCTT
    TTGTTAAAAT ATTCAAAAAA ATCTGAACTG TGGACGGCCC AGGAAACTAT TGTCTATTTG
    GGGGACTACT TGACTGTGAA GAAAAAAGGC AGACAAAGAA ATGCTTTTTG GGTTCATCAT
    CTTCATCAAG AAGAAATTCT GGGGAGGTAT GTTGGGAAAG ACTATAAGGA GCAGAAGGGG
    CTCTGGCACC ACTTCACTGA TGTGGAGCGA CAGATGACCG CACAGCACTA TGTGACAGAA
    TTTAACAAGA GACTCTATGA ACAAAACATT CCCACCCAGA TATTCTACAT CCCATCCACA
    ATACTACTGA TTTTAGAGGA CAAGACAATA AAGGGATGTA TCAGTGTGGA GCCTTACATA
    CTGGGAGAAT TTGTAAAATT GTCAAATAAC ACGAAAGTGG TGAAAACAGA ATACAAAGCC
    ACAGAATATG GCTTGGCCTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT
    GTGGTCGATT TACAAGGTTG GGTAACCGGT AATGGAAAAG GACTCATCTA CCTCACAGAT
    CCCCAGATTC ACTCCGTTGA TCAGAAAGTT TTCACTACCA ATTTTGGAAA GAGAGGAATT
    TTTTACTTCT TTAATAACCA GCATGTGGAA TGTAATGAAA TCTGCCATCG TCTTTCTTTG
    ACTAGACCTT CAATGGAGAA ACCATGCACA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95413
    Clone ID Related Accession (Same CDS sequence) XM_017008643.1
    Accession Version XM_017008643.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3696bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGCCTCC TGTTTTCTCA CAGAGCCTGC TGAAGGAGTT TGACCACCAT
    TTGCTGTCCG CTGCAGAAGC CTGCAAGCTG GCAGCTGCCT TCAGTGCCTA TACGCCGCTC
    TTCGTGCTCA CAGCTGTGAA TATCCGTGGC ACGTGTTTAT TGTCCTACAG TAGTTCAAAT
    GACTGTCCTC CAGAATTGAA AAACTTACAT CTGTGTGAAG CCAAAGAGGC CTTTGAGATT
    GGCCTCCTCA CCAAGAGAGA TGATGAGCCT GTTACTGGAA AACAGGAGCT TCACAGCTTT
    GTCAAAGCTG CTTTCGGTCT CACCACAGTG CACAGAAGGC TCCATGGGGA GACAGGGACG
    GTCCATGCAG CAAGTCAGCT CTGTAAGGAA GCAATGGGGA AGCTGTACAA TTTCAGCACT
    TCCTCCAGAA GTCAGGACAG AGAAGCTCTG TCTCAAGAAG TTATGTCTGT GATTGCCCAG
    GTGAAGGAAC ATTTACAAGT TCAAAGCTTC TCAAATGTAG ATGACAGATC TTATGTTCCC
    GAGAGTTTCG AGTGCAGGTT GGATAAACTT ATCTTGCATG GGCAAGGGGA TTTCCAAAAA
    ATCCTTGACA CCTATTCACA GCACCATACT TCGGTGTGTG AAGTATTTGA AAGTGATTGT
    GGAAACAACA AAAATGAACA GAAAGATGCA AAAACAGGAG TCTGCATCAC TGCTCTAAAA
    ACAGAAATAA AAAACATAGA TACTGTGAGT ACTACTCAAG AAAAGCCACA TTGTCAAAGA
    GACACAGGAA TATCTTCCTC CCTAATGGGT AAGAATGTTC AGAGGGAACT CAGAAGGGGA
    GGAAGGAGAA ACTGGACCCA TTCTGATGCA TTTCGAGTCT CCTTGGATCA AGATGTGGAG
    ACTGAGACTG AGCCATCGGA CTACAGCAAT GGTGAGGGAG CTGTTTTCAA CAAGTCTCTG
    AGTGGCAGCC AGACTTCCAG TGCTTGGAGC AACTTATCAG GGTTTAGTTC CTCTGCAAGC
    TGGGAGGAAG TGAATTATCA CGTTGACGAC AGGTCAGCCA GAAAAGAGCC TGGCAAAGAA
    CATCTGGTGG ACACTCAGTG TTCCACTGCC TTGTCTGAGG AGCTAGAGAA TGACAGGGAA
    GGCAGAGCTA TGCATTCATT GCATTCACAG CTTCATGATC TCTCTCTTCA GGAACCCAAC
    AATGACAATT TGGAGCCTTC TCAAAATCAG CCACAGCAAC AGATGCCCTT GACACCCTTC
    TCGCCTCATA ATACCCCAGG CATTTTCTTG GCCCCTGGTG CAGGGCTTCT AGAAGGAGCT
    CCAGAAGGTA TCCAGGAAGT CAGAAATATG GGACCCAGAA ATACTTCTGC TCACTCCAGA
    CCCTCATATC GTTCTGCTTC TTGGTCTTCT GATTCTGGTA GGCCCAAGAA TATGGGCACA
    CATCCTTCAG TCCAAAAAGA AGAAGCCTTT GAAATAATTG TTGAGTTTCC AGAAACCAAC
    TGCGATGTCA AAGACAGGCA GGGGAAAGAG CAGGGAGAAG AAATTAGTGA AAGAGGCGCA
    GGCCCTACAT TTAAAGCTAG TCCCTCCTGG GTTGACCCAG AAGGAGAAAC AGCAGAAAGC
    ACTGAAGATG CACCCTTAGA CTTTCACAGG GTCCTGCACA ATTCTCTGGG AAACATTTCC
    ATGCTGCCAT GTAGCTCCTT CACCCCTAAT TGGCCTGTTC AAAATCCTGA CTCCAGAAAA
    AGTGGTGGCC CAGTCGCAGA GCAGGGCATC GACCCTGATG CCTCCACAGT GGATGAGGAG
    GGGCAACTGC TCGACAGCAT GGATGTTCCC TGCACAAATG GGCACGGCTC TCATAGACTG
    TGCATTCTGA GACAGCCGCC TGGTCAGAGG GCGGAGACCC CCAATTCCTC TGTAAGCGGT
    AACATCCTCT TCCCTGTCCT CAGCGAGGAC TGCACTACCA CAGAGGAAGG AAATCAGCCT
    GGAAACATGC TAAACTGCAG CCAGAACTCC AGCTCATCCT CAGTGTGGTG GCTGAAATCA
    CCTGCATTTT CCAGTGGTTC TTCTGAGGGG GACAGCCCTT GGTCCTATCT GAATTCCAGT
    GGGAGTTCTT GGGTTTCATT GCCGGGAAAG ATGAGGAAAG AGATCCTTGA GGCTCGCACC
    TTGCAACCTG ATGACTTTGA AAAGCTGTTG GCAGGAGTGA GGCATGATTG GCTGTTTCAG
    AGACTAGAGA ATACGGGGGT TTTTAAGCCC AGTCAACTCC ACCGAGCACA TAGTGCTCTT
    TTGTTAAAAT ATTCAAAAAA ATCTGAACTG TGGACGGCCC AGGAAACTAT TGTCTATTTG
    GGGGACTACT TGACTGTGAA GAAAAAAGGC AGACAAAGAA ATGCTTTTTG GGTTCATCAT
    CTTCATCAAG AAGAAATTCT GGGGAGGTAT GTTGGGAAAG ACTATAAGGA GCAGAAGGGG
    CTCTGGCACC ACTTCACTGA TGTGGAGCGA CAGATGACCG CACAGCACTA TGTGACAGAA
    TTTAACAAGA GACTCTATGA ACAAAACATT CCCACCCAGA TATTCTACAT CCCATCCACA
    ATACTACTGA TTTTAGAGGA CAAGACAATA AAGGGATGTA TCAGTGTGGA GCCTTACATA
    CTGGGAGAAT TTGTAAAATT GTCAAATAAC ACGAAAGTGG TGAAAACAGA ATACAAAGCC
    ACAGAATATG GCTTGGCCTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT
    GTGGTCGATT TACAAGGTTG GGTAACCGGT AATGGAAAAG GACTCATCTA CCTCACAGAT
    CCCCAGATTC ACTCCGTTGA TCAGAAAGTT TTCACTACCA ATTTTGGAAA GAGAGGAATT
    TTTTACTTCT TTAATAACCA GCATGTGGAA TGTAATGAAA TCTGCCATCG TCTTTCTTTG
    ACTAGACCTT CAATGGAGAA ACCATGTAAG TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864132.1
    CDS280..3975
    Translation

    Target ORF information:

    RefSeq Version XM_017008643.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008643.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGCCTCC TGTTTTCTCA CAGAGCCTGC TGAAGGAGTT TGACCACCAT
    TTGCTGTCCG CTGCAGAAGC CTGCAAGCTG GCAGCTGCCT TCAGTGCCTA TACGCCGCTC
    TTCGTGCTCA CAGCTGTGAA TATCCGTGGC ACGTGTTTAT TGTCCTACAG TAGTTCAAAT
    GACTGTCCTC CAGAATTGAA AAACTTACAT CTGTGTGAAG CCAAAGAGGC CTTTGAGATT
    GGCCTCCTCA CCAAGAGAGA TGATGAGCCT GTTACTGGAA AACAGGAGCT TCACAGCTTT
    GTCAAAGCTG CTTTCGGTCT CACCACAGTG CACAGAAGGC TCCATGGGGA GACAGGGACG
    GTCCATGCAG CAAGTCAGCT CTGTAAGGAA GCAATGGGGA AGCTGTACAA TTTCAGCACT
    TCCTCCAGAA GTCAGGACAG AGAAGCTCTG TCTCAAGAAG TTATGTCTGT GATTGCCCAG
    GTGAAGGAAC ATTTACAAGT TCAAAGCTTC TCAAATGTAG ATGACAGATC TTATGTTCCC
    GAGAGTTTCG AGTGCAGGTT GGATAAACTT ATCTTGCATG GGCAAGGGGA TTTCCAAAAA
    ATCCTTGACA CCTATTCACA GCACCATACT TCGGTGTGTG AAGTATTTGA AAGTGATTGT
    GGAAACAACA AAAATGAACA GAAAGATGCA AAAACAGGAG TCTGCATCAC TGCTCTAAAA
    ACAGAAATAA AAAACATAGA TACTGTGAGT ACTACTCAAG AAAAGCCACA TTGTCAAAGA
    GACACAGGAA TATCTTCCTC CCTAATGGGT AAGAATGTTC AGAGGGAACT CAGAAGGGGA
    GGAAGGAGAA ACTGGACCCA TTCTGATGCA TTTCGAGTCT CCTTGGATCA AGATGTGGAG
    ACTGAGACTG AGCCATCGGA CTACAGCAAT GGTGAGGGAG CTGTTTTCAA CAAGTCTCTG
    AGTGGCAGCC AGACTTCCAG TGCTTGGAGC AACTTATCAG GGTTTAGTTC CTCTGCAAGC
    TGGGAGGAAG TGAATTATCA CGTTGACGAC AGGTCAGCCA GAAAAGAGCC TGGCAAAGAA
    CATCTGGTGG ACACTCAGTG TTCCACTGCC TTGTCTGAGG AGCTAGAGAA TGACAGGGAA
    GGCAGAGCTA TGCATTCATT GCATTCACAG CTTCATGATC TCTCTCTTCA GGAACCCAAC
    AATGACAATT TGGAGCCTTC TCAAAATCAG CCACAGCAAC AGATGCCCTT GACACCCTTC
    TCGCCTCATA ATACCCCAGG CATTTTCTTG GCCCCTGGTG CAGGGCTTCT AGAAGGAGCT
    CCAGAAGGTA TCCAGGAAGT CAGAAATATG GGACCCAGAA ATACTTCTGC TCACTCCAGA
    CCCTCATATC GTTCTGCTTC TTGGTCTTCT GATTCTGGTA GGCCCAAGAA TATGGGCACA
    CATCCTTCAG TCCAAAAAGA AGAAGCCTTT GAAATAATTG TTGAGTTTCC AGAAACCAAC
    TGCGATGTCA AAGACAGGCA GGGGAAAGAG CAGGGAGAAG AAATTAGTGA AAGAGGCGCA
    GGCCCTACAT TTAAAGCTAG TCCCTCCTGG GTTGACCCAG AAGGAGAAAC AGCAGAAAGC
    ACTGAAGATG CACCCTTAGA CTTTCACAGG GTCCTGCACA ATTCTCTGGG AAACATTTCC
    ATGCTGCCAT GTAGCTCCTT CACCCCTAAT TGGCCTGTTC AAAATCCTGA CTCCAGAAAA
    AGTGGTGGCC CAGTCGCAGA GCAGGGCATC GACCCTGATG CCTCCACAGT GGATGAGGAG
    GGGCAACTGC TCGACAGCAT GGATGTTCCC TGCACAAATG GGCACGGCTC TCATAGACTG
    TGCATTCTGA GACAGCCGCC TGGTCAGAGG GCGGAGACCC CCAATTCCTC TGTAAGCGGT
    AACATCCTCT TCCCTGTCCT CAGCGAGGAC TGCACTACCA CAGAGGAAGG AAATCAGCCT
    GGAAACATGC TAAACTGCAG CCAGAACTCC AGCTCATCCT CAGTGTGGTG GCTGAAATCA
    CCTGCATTTT CCAGTGGTTC TTCTGAGGGG GACAGCCCTT GGTCCTATCT GAATTCCAGT
    GGGAGTTCTT GGGTTTCATT GCCGGGAAAG ATGAGGAAAG AGATCCTTGA GGCTCGCACC
    TTGCAACCTG ATGACTTTGA AAAGCTGTTG GCAGGAGTGA GGCATGATTG GCTGTTTCAG
    AGACTAGAGA ATACGGGGGT TTTTAAGCCC AGTCAACTCC ACCGAGCACA TAGTGCTCTT
    TTGTTAAAAT ATTCAAAAAA ATCTGAACTG TGGACGGCCC AGGAAACTAT TGTCTATTTG
    GGGGACTACT TGACTGTGAA GAAAAAAGGC AGACAAAGAA ATGCTTTTTG GGTTCATCAT
    CTTCATCAAG AAGAAATTCT GGGGAGGTAT GTTGGGAAAG ACTATAAGGA GCAGAAGGGG
    CTCTGGCACC ACTTCACTGA TGTGGAGCGA CAGATGACCG CACAGCACTA TGTGACAGAA
    TTTAACAAGA GACTCTATGA ACAAAACATT CCCACCCAGA TATTCTACAT CCCATCCACA
    ATACTACTGA TTTTAGAGGA CAAGACAATA AAGGGATGTA TCAGTGTGGA GCCTTACATA
    CTGGGAGAAT TTGTAAAATT GTCAAATAAC ACGAAAGTGG TGAAAACAGA ATACAAAGCC
    ACAGAATATG GCTTGGCCTA TGGCCATTTT TCTTATGAGT TTTCTAATCA TAGAGATGTT
    GTGGTCGATT TACAAGGTTG GGTAACCGGT AATGGAAAAG GACTCATCTA CCTCACAGAT
    CCCCAGATTC ACTCCGTTGA TCAGAAAGTT TTCACTACCA ATTTTGGAAA GAGAGGAATT
    TTTTACTTCT TTAATAACCA GCATGTGGAA TGTAATGAAA TCTGCCATCG TCTTTCTTTG
    ACTAGACCTT CAATGGAGAA ACCATGTAAG TCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95414
    Clone ID Related Accession (Same CDS sequence) XM_017008644.1
    Accession Version XM_017008644.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3687bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTGGAAGT TCTCCCAGAT GATGATGATT GAAAGAAGAA AACAGCAGAA CAATATGTTT 
    AGTGTGACGT CATTTACGTA TGAGAGAGAA AGAGATAGGA AGGCTGTGGG TTTTGTTGTA
    TTAATGAAAA TGCCTCCCAC GCTAAGCCTG GCCCTCAGCC TGCCAGCGTT TCCGCCTGGC
    TCACGATGTT CCACGCTGTT CCTCCAGGCG TCCCTGAGGG CCTCCATCCT CGCTCGGGAC
    TGTGCGGCTG CGGCGGCTAT TGTGTTCTTG GTGGACCGGT TCCTGTATGG GCTCGACGTC
    TCTGGAAAAC TTCTGCAGGT CGCCAAAGGT CTCCACAAGT TGCAGCCAGC CACGCCAATT
    GCCCCGCAGG TGGTTATTCG CCAAGCCCGA ATCTCCGTGA ACTCAGGAAA ACTTTTAAAA
    GCAGAGTATA TTCTGAGCAG TCTAATAAGC AACAATGGAG CAACGGGTAC CTGGCTGTAC
    AGAAATGAAA GTGACAAGGT CCTGGTGCAG TCGGTCTGTA TACAGATCAG AGGGCAGATT
    CTGCAAAAGC TGGGGATGTG GTACGAAGCA GCAGAGTTAA TATGGGCCTC CATTGTAGGA
    TATTTGGCAC TTCCTCAGCC GGATAAAAAG GGCCTCTCCA CGTCGCTAGG TATACTGGCA
    GACATCTTTG TTTCCATGAG CAAGAACGAT TATGAAAAGT TTAAAAACAA TCCACAAATT
    AATTTGCCTC CTGTTTTCTC ACAGAGCCTG CTGAAGGAGT TTGACCACCA TTTGCTGTCC
    GCTGCAGAAG CCTGCAAGCT GGCAGCTGCC TTCAGTGCCT ATACGCCGCT CTTCGTGCTC
    ACAGCTGTGA ATATCCGTGG CACGTGTTTA TTGTCCTACA GTAGTTCAAA TGACTGTCCT
    CCAGAATTGA AAAACTTACA TCTGTGTGAA GCCAAAGAGG CCTTTGAGAT TGGCCTCCTC
    ACCAAGAGAG ATGATGAGCC TGTTACTGGA AAACAGGAGC TTCACAGCTT TGTCAAAGCT
    GCTTTCGGTC TCACCACAGT GCACAGAAGG CTCCATGGGG AGACAGGGAC GGTCCATGCA
    GCAAGTCAGC TCTGTAAGGA AGCAATGGGG AAGCTGTACA ATTTCAGCAC TTCCTCCAGA
    AGTCAGGACA GAGAAGCTCT GTCTCAAGAA GTTATGTCTG TGATTGCCCA GGTGAAGGAA
    CATTTACAAG TTCAAAGCTT CTCAAATGTA GATGACAGAT CTTATGTTCC CGAGAGTTTC
    GAGTGCAGGT TGGATAAACT TATCTTGCAT GGGCAAGGGG ATTTCCAAAA AATCCTTGAC
    ACCTATTCAC AGCACCATAC TTCGGTGTGT GAAGTATTTG AAAGTGATTG TGGAAACAAC
    AAAAATGAAC AGAAAGATGC AAAAACAGGA GTCTGCATCA CTGCTCTAAA AACAGAAATA
    AAAAACATAG ATACTGTGAG TACTACTCAA GAAAAGCCAC ATTGTCAAAG AGACACAGGA
    ATATCTTCCT CCCTAATGGG TAAGAATGTT CAGAGGGAAC TCAGAAGGGG AGGAAGGAGA
    AACTGGACCC ATTCTGATGC ATTTCGAGTC TCCTTGGATC AAGATGTGGA GACTGAGACT
    GAGCCATCGG ACTACAGCAA TGGTGAGGGA GCTGTTTTCA ACAAGTCTCT GAGTGGCAGC
    CAGACTTCCA GTGCTTGGAG CAACTTATCA GGGTTTAGTT CCTCTGCAAG CTGGGAGGAA
    GTGAATTATC ACGTTGACGA CAGGTCAGCC AGAAAAGAGC CTGGCAAAGA ACATCTGGTG
    GACACTCAGT GTTCCACTGC CTTGTCTGAG GAGCTAGAGA ATGACAGGGA AGGCAGAGCT
    ATGCATTCAT TGCATTCACA GCTTCATGAT CTCTCTCTTC AGGAACCCAA CAATGACAAT
    TTGGAGCCTT CTCAAAATCA GCCACAGCAA CAGATGCCCT TGACACCCTT CTCGCCTCAT
    AATACCCCAG GCATTTTCTT GGCCCCTGGT GCAGGGCTTC TAGAAGGAGC TCCAGAAGGT
    ATCCAGGAAG TCAGAAATAT GGGACCCAGA AATACTTCTG CTCACTCCAG ACCCTCATAT
    CGTTCTGCTT CTTGGTCTTC TGATTCTGGT AGGCCCAAGA ATATGGGCAC ACATCCTTCA
    GTCCAAAAAG AAGAAGCCTT TGAAATAATT GTTGAGTTTC CAGAAACCAA CTGCGATGTC
    AAAGACAGGC AGGGGAAAGA GCAGGGAGAA GAAATTAGTG AAAGAGGCGC AGGCCCTACA
    TTTAAAGCTA GTCCCTCCTG GGTTGACCCA GAAGGAGAAA CAGCAGAAAG CACTGAAGAT
    GCACCCTTAG ACTTTCACAG GGTCCTGCAC AATTCTCTGG GAAACATTTC CATGCTGCCA
    TGTAGCTCCT TCACCCCTAA TTGGCCTGTT CAAAATCCTG ACTCCAGAAA AAGTGGTGGC
    CCAGTCGCAG AGCAGGGCAT CGACCCTGAT GCCTCCACAG TGGATGAGGA GGGGCAACTG
    CTCGACAGCA TGGATGTTCC CTGCACAAAT GGGCACGGCT CTCATAGACT GTGCATTCTG
    AGACAGCCGC CTGGTCAGAG GGCGGAGACC CCCAATTCCT CTGTAAGCGG TAACATCCTC
    TTCCCTGTCC TCAGCGAGGA CTGCACTACC ACAGAGGAAG GAAATCAGCC TGGAAACATG
    CTAAACTGCA GCCAGAACTC CAGCTCATCC TCAGTGTGGT GGCTGAAATC ACCTGCATTT
    TCCAGTGGTT CTTCTGAGGG GGACAGCCCT TGGTCCTATC TGAATTCCAG TGGGAGTTCT
    TGGGTTTCAT TGCCGGGAAA GATGAGGAAA GAGATCCTTG AGGCTCGCAC CTTGCAACCT
    GATGACTTTG AAAAGCTGTT GGCAGGAGTG AGGCATGATT GGCTGTTTCA GAGACTAGAG
    AATACGGGGG TTTTTAAGCC CAGTCAACTC CACCGAGCAC ATAGTGCTCT TTTGTTAAAA
    TATTCAAAAA AATCTGAACT GTGGACGGCC CAGGAAACTA TTGTCTATTT GGGGGACTAC
    TTGACTGTGA AGAAAAAAGG CAGACAAAGA AATGCTTTTT GGGTTCATCA TCTTCATCAA
    GAAGAAATTC TGGGGAGGTA TGTTGGGAAA GACTATAAGG AGCAGAAGGG GCTCTGGCAC
    CACTTCACTG ATGTGGAGCG ACAGATGACC GCACAGCACT ATGTGACAGA ATTTAACAAG
    AGACTCTATG AACAAAACAT TCCCACCCAG ATATTCTACA TCCCATCCAC AATACTACTG
    ATTTTAGAGG ACAAGACAAT AAAGGGATGT ATCAGTGTGG AGCCTTACAT ACTGGGAGAA
    TTTGTAAAAT TGTCAAATAA CACGAAAGTG GTGAAAACAG AATACAAAGC CACAGAATAT
    GGCTTGGCCT ATGGCCATTT TTCTTATGAG TTTTCTAATC ATAGAGATGT TGTGGTCGAT
    TTACAAGGTT GGGTAACCGG TAATGGAAAA GGACTCATCT ACCTCACAGA TCCCCAGATT
    CACTCCGTTG ATCAGAAAGT TTTCACTACC AATTTTGGAA AGAGAGGAAT TTTTTACTTC
    TTTAATAACC AGCATGTGGA ATGTAATGAA ATCTGCCATC GTCTTTCTTT GACTAGACCT
    TCAATGGAGA AACCATGTAA GTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864133.1
    CDS3482..7168
    Translation

    Target ORF information:

    RefSeq Version XM_017008644.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008644.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTGGAAGT TCTCCCAGAT GATGATGATT GAAAGAAGAA AACAGCAGAA CAATATGTTT 
    AGTGTGACGT CATTTACGTA TGAGAGAGAA AGAGATAGGA AGGCTGTGGG TTTTGTTGTA
    TTAATGAAAA TGCCTCCCAC GCTAAGCCTG GCCCTCAGCC TGCCAGCGTT TCCGCCTGGC
    TCACGATGTT CCACGCTGTT CCTCCAGGCG TCCCTGAGGG CCTCCATCCT CGCTCGGGAC
    TGTGCGGCTG CGGCGGCTAT TGTGTTCTTG GTGGACCGGT TCCTGTATGG GCTCGACGTC
    TCTGGAAAAC TTCTGCAGGT CGCCAAAGGT CTCCACAAGT TGCAGCCAGC CACGCCAATT
    GCCCCGCAGG TGGTTATTCG CCAAGCCCGA ATCTCCGTGA ACTCAGGAAA ACTTTTAAAA
    GCAGAGTATA TTCTGAGCAG TCTAATAAGC AACAATGGAG CAACGGGTAC CTGGCTGTAC
    AGAAATGAAA GTGACAAGGT CCTGGTGCAG TCGGTCTGTA TACAGATCAG AGGGCAGATT
    CTGCAAAAGC TGGGGATGTG GTACGAAGCA GCAGAGTTAA TATGGGCCTC CATTGTAGGA
    TATTTGGCAC TTCCTCAGCC GGATAAAAAG GGCCTCTCCA CGTCGCTAGG TATACTGGCA
    GACATCTTTG TTTCCATGAG CAAGAACGAT TATGAAAAGT TTAAAAACAA TCCACAAATT
    AATTTGCCTC CTGTTTTCTC ACAGAGCCTG CTGAAGGAGT TTGACCACCA TTTGCTGTCC
    GCTGCAGAAG CCTGCAAGCT GGCAGCTGCC TTCAGTGCCT ATACGCCGCT CTTCGTGCTC
    ACAGCTGTGA ATATCCGTGG CACGTGTTTA TTGTCCTACA GTAGTTCAAA TGACTGTCCT
    CCAGAATTGA AAAACTTACA TCTGTGTGAA GCCAAAGAGG CCTTTGAGAT TGGCCTCCTC
    ACCAAGAGAG ATGATGAGCC TGTTACTGGA AAACAGGAGC TTCACAGCTT TGTCAAAGCT
    GCTTTCGGTC TCACCACAGT GCACAGAAGG CTCCATGGGG AGACAGGGAC GGTCCATGCA
    GCAAGTCAGC TCTGTAAGGA AGCAATGGGG AAGCTGTACA ATTTCAGCAC TTCCTCCAGA
    AGTCAGGACA GAGAAGCTCT GTCTCAAGAA GTTATGTCTG TGATTGCCCA GGTGAAGGAA
    CATTTACAAG TTCAAAGCTT CTCAAATGTA GATGACAGAT CTTATGTTCC CGAGAGTTTC
    GAGTGCAGGT TGGATAAACT TATCTTGCAT GGGCAAGGGG ATTTCCAAAA AATCCTTGAC
    ACCTATTCAC AGCACCATAC TTCGGTGTGT GAAGTATTTG AAAGTGATTG TGGAAACAAC
    AAAAATGAAC AGAAAGATGC AAAAACAGGA GTCTGCATCA CTGCTCTAAA AACAGAAATA
    AAAAACATAG ATACTGTGAG TACTACTCAA GAAAAGCCAC ATTGTCAAAG AGACACAGGA
    ATATCTTCCT CCCTAATGGG TAAGAATGTT CAGAGGGAAC TCAGAAGGGG AGGAAGGAGA
    AACTGGACCC ATTCTGATGC ATTTCGAGTC TCCTTGGATC AAGATGTGGA GACTGAGACT
    GAGCCATCGG ACTACAGCAA TGGTGAGGGA GCTGTTTTCA ACAAGTCTCT GAGTGGCAGC
    CAGACTTCCA GTGCTTGGAG CAACTTATCA GGGTTTAGTT CCTCTGCAAG CTGGGAGGAA
    GTGAATTATC ACGTTGACGA CAGGTCAGCC AGAAAAGAGC CTGGCAAAGA ACATCTGGTG
    GACACTCAGT GTTCCACTGC CTTGTCTGAG GAGCTAGAGA ATGACAGGGA AGGCAGAGCT
    ATGCATTCAT TGCATTCACA GCTTCATGAT CTCTCTCTTC AGGAACCCAA CAATGACAAT
    TTGGAGCCTT CTCAAAATCA GCCACAGCAA CAGATGCCCT TGACACCCTT CTCGCCTCAT
    AATACCCCAG GCATTTTCTT GGCCCCTGGT GCAGGGCTTC TAGAAGGAGC TCCAGAAGGT
    ATCCAGGAAG TCAGAAATAT GGGACCCAGA AATACTTCTG CTCACTCCAG ACCCTCATAT
    CGTTCTGCTT CTTGGTCTTC TGATTCTGGT AGGCCCAAGA ATATGGGCAC ACATCCTTCA
    GTCCAAAAAG AAGAAGCCTT TGAAATAATT GTTGAGTTTC CAGAAACCAA CTGCGATGTC
    AAAGACAGGC AGGGGAAAGA GCAGGGAGAA GAAATTAGTG AAAGAGGCGC AGGCCCTACA
    TTTAAAGCTA GTCCCTCCTG GGTTGACCCA GAAGGAGAAA CAGCAGAAAG CACTGAAGAT
    GCACCCTTAG ACTTTCACAG GGTCCTGCAC AATTCTCTGG GAAACATTTC CATGCTGCCA
    TGTAGCTCCT TCACCCCTAA TTGGCCTGTT CAAAATCCTG ACTCCAGAAA AAGTGGTGGC
    CCAGTCGCAG AGCAGGGCAT CGACCCTGAT GCCTCCACAG TGGATGAGGA GGGGCAACTG
    CTCGACAGCA TGGATGTTCC CTGCACAAAT GGGCACGGCT CTCATAGACT GTGCATTCTG
    AGACAGCCGC CTGGTCAGAG GGCGGAGACC CCCAATTCCT CTGTAAGCGG TAACATCCTC
    TTCCCTGTCC TCAGCGAGGA CTGCACTACC ACAGAGGAAG GAAATCAGCC TGGAAACATG
    CTAAACTGCA GCCAGAACTC CAGCTCATCC TCAGTGTGGT GGCTGAAATC ACCTGCATTT
    TCCAGTGGTT CTTCTGAGGG GGACAGCCCT TGGTCCTATC TGAATTCCAG TGGGAGTTCT
    TGGGTTTCAT TGCCGGGAAA GATGAGGAAA GAGATCCTTG AGGCTCGCAC CTTGCAACCT
    GATGACTTTG AAAAGCTGTT GGCAGGAGTG AGGCATGATT GGCTGTTTCA GAGACTAGAG
    AATACGGGGG TTTTTAAGCC CAGTCAACTC CACCGAGCAC ATAGTGCTCT TTTGTTAAAA
    TATTCAAAAA AATCTGAACT GTGGACGGCC CAGGAAACTA TTGTCTATTT GGGGGACTAC
    TTGACTGTGA AGAAAAAAGG CAGACAAAGA AATGCTTTTT GGGTTCATCA TCTTCATCAA
    GAAGAAATTC TGGGGAGGTA TGTTGGGAAA GACTATAAGG AGCAGAAGGG GCTCTGGCAC
    CACTTCACTG ATGTGGAGCG ACAGATGACC GCACAGCACT ATGTGACAGA ATTTAACAAG
    AGACTCTATG AACAAAACAT TCCCACCCAG ATATTCTACA TCCCATCCAC AATACTACTG
    ATTTTAGAGG ACAAGACAAT AAAGGGATGT ATCAGTGTGG AGCCTTACAT ACTGGGAGAA
    TTTGTAAAAT TGTCAAATAA CACGAAAGTG GTGAAAACAG AATACAAAGC CACAGAATAT
    GGCTTGGCCT ATGGCCATTT TTCTTATGAG TTTTCTAATC ATAGAGATGT TGTGGTCGAT
    TTACAAGGTT GGGTAACCGG TAATGGAAAA GGACTCATCT ACCTCACAGA TCCCCAGATT
    CACTCCGTTG ATCAGAAAGT TTTCACTACC AATTTTGGAA AGAGAGGAAT TTTTTACTTC
    TTTAATAACC AGCATGTGGA ATGTAATGAA ATCTGCCATC GTCTTTCTTT GACTAGACCT
    TCAATGGAGA AACCATGTAA GTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95415
    Clone ID Related Accession (Same CDS sequence) XM_017008645.1
    Accession Version XM_017008645.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3675bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGAGCCT GCTGAAGGAG TTTGACCACC ATTTGCTGTC CGCTGCAGAA
    GCCTGCAAGC TGGCAGCTGC CTTCAGTGCC TATACGCCGC TCTTCGTGCT CACAGCTGTG
    AATATCCGTG GCACGTGTTT ATTGTCCTAC AGTAGTTCAA ATGACTGTCC TCCAGAATTG
    AAAAACTTAC ATCTGTGTGA AGCCAAAGAG GCCTTTGAGA TTGGCCTCCT CACCAAGAGA
    GATGATGAGC CTGTTACTGG AAAACAGGAG CTTCACAGCT TTGTCAAAGC TGCTTTCGGT
    CTCACCACAG TGCACAGAAG GCTCCATGGG GAGACAGGGA CGGTCCATGC AGCAAGTCAG
    CTCTGTAAGG AAGCAATGGG GAAGCTGTAC AATTTCAGCA CTTCCTCCAG AAGTCAGGAC
    AGAGAAGCTC TGTCTCAAGA AGTTATGTCT GTGATTGCCC AGGTGAAGGA ACATTTACAA
    GTTCAAAGCT TCTCAAATGT AGATGACAGA TCTTATGTTC CCGAGAGTTT CGAGTGCAGG
    TTGGATAAAC TTATCTTGCA TGGGCAAGGG GATTTCCAAA AAATCCTTGA CACCTATTCA
    CAGCACCATA CTTCGGTGTG TGAAGTATTT GAAAGTGATT GTGGAAACAA CAAAAATGAA
    CAGAAAGATG CAAAAACAGG AGTCTGCATC ACTGCTCTAA AAACAGAAAT AAAAAACATA
    GATACTGTGA GTACTACTCA AGAAAAGCCA CATTGTCAAA GAGACACAGG AATATCTTCC
    TCCCTAATGG GTAAGAATGT TCAGAGGGAA CTCAGAAGGG GAGGAAGGAG AAACTGGACC
    CATTCTGATG CATTTCGAGT CTCCTTGGAT CAAGATGTGG AGACTGAGAC TGAGCCATCG
    GACTACAGCA ATGGTGAGGG AGCTGTTTTC AACAAGTCTC TGAGTGGCAG CCAGACTTCC
    AGTGCTTGGA GCAACTTATC AGGGTTTAGT TCCTCTGCAA GCTGGGAGGA AGTGAATTAT
    CACGTTGACG ACAGGTCAGC CAGAAAAGAG CCTGGCAAAG AACATCTGGT GGACACTCAG
    TGTTCCACTG CCTTGTCTGA GGAGCTAGAG AATGACAGGG AAGGCAGAGC TATGCATTCA
    TTGCATTCAC AGCTTCATGA TCTCTCTCTT CAGGAACCCA ACAATGACAA TTTGGAGCCT
    TCTCAAAATC AGCCACAGCA ACAGATGCCC TTGACACCCT TCTCGCCTCA TAATACCCCA
    GGCATTTTCT TGGCCCCTGG TGCAGGGCTT CTAGAAGGAG CTCCAGAAGG TATCCAGGAA
    GTCAGAAATA TGGGACCCAG AAATACTTCT GCTCACTCCA GACCCTCATA TCGTTCTGCT
    TCTTGGTCTT CTGATTCTGG TAGGCCCAAG AATATGGGCA CACATCCTTC AGTCCAAAAA
    GAAGAAGCCT TTGAAATAAT TGTTGAGTTT CCAGAAACCA ACTGCGATGT CAAAGACAGG
    CAGGGGAAAG AGCAGGGAGA AGAAATTAGT GAAAGAGGCG CAGGCCCTAC ATTTAAAGCT
    AGTCCCTCCT GGGTTGACCC AGAAGGAGAA ACAGCAGAAA GCACTGAAGA TGCACCCTTA
    GACTTTCACA GGGTCCTGCA CAATTCTCTG GGAAACATTT CCATGCTGCC ATGTAGCTCC
    TTCACCCCTA ATTGGCCTGT TCAAAATCCT GACTCCAGAA AAAGTGGTGG CCCAGTCGCA
    GAGCAGGGCA TCGACCCTGA TGCCTCCACA GTGGATGAGG AGGGGCAACT GCTCGACAGC
    ATGGATGTTC CCTGCACAAA TGGGCACGGC TCTCATAGAC TGTGCATTCT GAGACAGCCG
    CCTGGTCAGA GGGCGGAGAC CCCCAATTCC TCTGTAAGCG GTAACATCCT CTTCCCTGTC
    CTCAGCGAGG ACTGCACTAC CACAGAGGAA GGAAATCAGC CTGGAAACAT GCTAAACTGC
    AGCCAGAACT CCAGCTCATC CTCAGTGTGG TGGCTGAAAT CACCTGCATT TTCCAGTGGT
    TCTTCTGAGG GGGACAGCCC TTGGTCCTAT CTGAATTCCA GTGGGAGTTC TTGGGTTTCA
    TTGCCGGGAA AGATGAGGAA AGAGATCCTT GAGGCTCGCA CCTTGCAACC TGATGACTTT
    GAAAAGCTGT TGGCAGGAGT GAGGCATGAT TGGCTGTTTC AGAGACTAGA GAATACGGGG
    GTTTTTAAGC CCAGTCAACT CCACCGAGCA CATAGTGCTC TTTTGTTAAA ATATTCAAAA
    AAATCTGAAC TGTGGACGGC CCAGGAAACT ATTGTCTATT TGGGGGACTA CTTGACTGTG
    AAGAAAAAAG GCAGACAAAG AAATGCTTTT TGGGTTCATC ATCTTCATCA AGAAGAAATT
    CTGGGGAGGT ATGTTGGGAA AGACTATAAG GAGCAGAAGG GGCTCTGGCA CCACTTCACT
    GATGTGGAGC GACAGATGAC CGCACAGCAC TATGTGACAG AATTTAACAA GAGACTCTAT
    GAACAAAACA TTCCCACCCA GATATTCTAC ATCCCATCCA CAATACTACT GATTTTAGAG
    GACAAGACAA TAAAGGGATG TATCAGTGTG GAGCCTTACA TACTGGGAGA ATTTGTAAAA
    TTGTCAAATA ACACGAAAGT GGTGAAAACA GAATACAAAG CCACAGAATA TGGCTTGGCC
    TATGGCCATT TTTCTTATGA GTTTTCTAAT CATAGAGATG TTGTGGTCGA TTTACAAGGT
    TGGGTAACCG GTAATGGAAA AGGACTCATC TACCTCACAG ATCCCCAGAT TCACTCCGTT
    GATCAGAAAG TTTTCACTAC CAATTTTGGA AAGAGAGGAA TTTTTTACTT CTTTAATAAC
    CAGCATGTGG AATGTAATGA AATCTGCCAT CGTCTTTCTT TGACTAGACC TTCAATGGAG
    AAACCATGCA CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864134.1
    CDS280..3954
    Translation

    Target ORF information:

    RefSeq Version XM_017008645.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008645.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGAGCCT GCTGAAGGAG TTTGACCACC ATTTGCTGTC CGCTGCAGAA
    GCCTGCAAGC TGGCAGCTGC CTTCAGTGCC TATACGCCGC TCTTCGTGCT CACAGCTGTG
    AATATCCGTG GCACGTGTTT ATTGTCCTAC AGTAGTTCAA ATGACTGTCC TCCAGAATTG
    AAAAACTTAC ATCTGTGTGA AGCCAAAGAG GCCTTTGAGA TTGGCCTCCT CACCAAGAGA
    GATGATGAGC CTGTTACTGG AAAACAGGAG CTTCACAGCT TTGTCAAAGC TGCTTTCGGT
    CTCACCACAG TGCACAGAAG GCTCCATGGG GAGACAGGGA CGGTCCATGC AGCAAGTCAG
    CTCTGTAAGG AAGCAATGGG GAAGCTGTAC AATTTCAGCA CTTCCTCCAG AAGTCAGGAC
    AGAGAAGCTC TGTCTCAAGA AGTTATGTCT GTGATTGCCC AGGTGAAGGA ACATTTACAA
    GTTCAAAGCT TCTCAAATGT AGATGACAGA TCTTATGTTC CCGAGAGTTT CGAGTGCAGG
    TTGGATAAAC TTATCTTGCA TGGGCAAGGG GATTTCCAAA AAATCCTTGA CACCTATTCA
    CAGCACCATA CTTCGGTGTG TGAAGTATTT GAAAGTGATT GTGGAAACAA CAAAAATGAA
    CAGAAAGATG CAAAAACAGG AGTCTGCATC ACTGCTCTAA AAACAGAAAT AAAAAACATA
    GATACTGTGA GTACTACTCA AGAAAAGCCA CATTGTCAAA GAGACACAGG AATATCTTCC
    TCCCTAATGG GTAAGAATGT TCAGAGGGAA CTCAGAAGGG GAGGAAGGAG AAACTGGACC
    CATTCTGATG CATTTCGAGT CTCCTTGGAT CAAGATGTGG AGACTGAGAC TGAGCCATCG
    GACTACAGCA ATGGTGAGGG AGCTGTTTTC AACAAGTCTC TGAGTGGCAG CCAGACTTCC
    AGTGCTTGGA GCAACTTATC AGGGTTTAGT TCCTCTGCAA GCTGGGAGGA AGTGAATTAT
    CACGTTGACG ACAGGTCAGC CAGAAAAGAG CCTGGCAAAG AACATCTGGT GGACACTCAG
    TGTTCCACTG CCTTGTCTGA GGAGCTAGAG AATGACAGGG AAGGCAGAGC TATGCATTCA
    TTGCATTCAC AGCTTCATGA TCTCTCTCTT CAGGAACCCA ACAATGACAA TTTGGAGCCT
    TCTCAAAATC AGCCACAGCA ACAGATGCCC TTGACACCCT TCTCGCCTCA TAATACCCCA
    GGCATTTTCT TGGCCCCTGG TGCAGGGCTT CTAGAAGGAG CTCCAGAAGG TATCCAGGAA
    GTCAGAAATA TGGGACCCAG AAATACTTCT GCTCACTCCA GACCCTCATA TCGTTCTGCT
    TCTTGGTCTT CTGATTCTGG TAGGCCCAAG AATATGGGCA CACATCCTTC AGTCCAAAAA
    GAAGAAGCCT TTGAAATAAT TGTTGAGTTT CCAGAAACCA ACTGCGATGT CAAAGACAGG
    CAGGGGAAAG AGCAGGGAGA AGAAATTAGT GAAAGAGGCG CAGGCCCTAC ATTTAAAGCT
    AGTCCCTCCT GGGTTGACCC AGAAGGAGAA ACAGCAGAAA GCACTGAAGA TGCACCCTTA
    GACTTTCACA GGGTCCTGCA CAATTCTCTG GGAAACATTT CCATGCTGCC ATGTAGCTCC
    TTCACCCCTA ATTGGCCTGT TCAAAATCCT GACTCCAGAA AAAGTGGTGG CCCAGTCGCA
    GAGCAGGGCA TCGACCCTGA TGCCTCCACA GTGGATGAGG AGGGGCAACT GCTCGACAGC
    ATGGATGTTC CCTGCACAAA TGGGCACGGC TCTCATAGAC TGTGCATTCT GAGACAGCCG
    CCTGGTCAGA GGGCGGAGAC CCCCAATTCC TCTGTAAGCG GTAACATCCT CTTCCCTGTC
    CTCAGCGAGG ACTGCACTAC CACAGAGGAA GGAAATCAGC CTGGAAACAT GCTAAACTGC
    AGCCAGAACT CCAGCTCATC CTCAGTGTGG TGGCTGAAAT CACCTGCATT TTCCAGTGGT
    TCTTCTGAGG GGGACAGCCC TTGGTCCTAT CTGAATTCCA GTGGGAGTTC TTGGGTTTCA
    TTGCCGGGAA AGATGAGGAA AGAGATCCTT GAGGCTCGCA CCTTGCAACC TGATGACTTT
    GAAAAGCTGT TGGCAGGAGT GAGGCATGAT TGGCTGTTTC AGAGACTAGA GAATACGGGG
    GTTTTTAAGC CCAGTCAACT CCACCGAGCA CATAGTGCTC TTTTGTTAAA ATATTCAAAA
    AAATCTGAAC TGTGGACGGC CCAGGAAACT ATTGTCTATT TGGGGGACTA CTTGACTGTG
    AAGAAAAAAG GCAGACAAAG AAATGCTTTT TGGGTTCATC ATCTTCATCA AGAAGAAATT
    CTGGGGAGGT ATGTTGGGAA AGACTATAAG GAGCAGAAGG GGCTCTGGCA CCACTTCACT
    GATGTGGAGC GACAGATGAC CGCACAGCAC TATGTGACAG AATTTAACAA GAGACTCTAT
    GAACAAAACA TTCCCACCCA GATATTCTAC ATCCCATCCA CAATACTACT GATTTTAGAG
    GACAAGACAA TAAAGGGATG TATCAGTGTG GAGCCTTACA TACTGGGAGA ATTTGTAAAA
    TTGTCAAATA ACACGAAAGT GGTGAAAACA GAATACAAAG CCACAGAATA TGGCTTGGCC
    TATGGCCATT TTTCTTATGA GTTTTCTAAT CATAGAGATG TTGTGGTCGA TTTACAAGGT
    TGGGTAACCG GTAATGGAAA AGGACTCATC TACCTCACAG ATCCCCAGAT TCACTCCGTT
    GATCAGAAAG TTTTCACTAC CAATTTTGGA AAGAGAGGAA TTTTTTACTT CTTTAATAAC
    CAGCATGTGG AATGTAATGA AATCTGCCAT CGTCTTTCTT TGACTAGACC TTCAATGGAG
    AAACCATGCA CATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95416
    Clone ID Related Accession (Same CDS sequence) XM_017008646.1
    Accession Version XM_017008646.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGAATAT CCGTGGCACG TGTTTATTGT CCTACAGTAG TTCAAATGAC
    TGTCCTCCAG AATTGAAAAA CTTACATCTG TGTGAAGCCA AAGAGGCCTT TGAGATTGGC
    CTCCTCACCA AGAGAGATGA TGAGCCTGTT ACTGGAAAAC AGGAGCTTCA CAGCTTTGTC
    AAAGCTGCTT TCGGTCTCAC CACAGTGCAC AGAAGGCTCC ATGGGGAGAC AGGGACGGTC
    CATGCAGCAA GTCAGCTCTG TAAGGAAGCA ATGGGGAAGC TGTACAATTT CAGCACTTCC
    TCCAGAAGTC AGGACAGAGA AGCTCTGTCT CAAGAAGTTA TGTCTGTGAT TGCCCAGGTG
    AAGGAACATT TACAAGTTCA AAGCTTCTCA AATGTAGATG ACAGATCTTA TGTTCCCGAG
    AGTTTCGAGT GCAGGTTGGA TAAACTTATC TTGCATGGGC AAGGGGATTT CCAAAAAATC
    CTTGACACCT ATTCACAGCA CCATACTTCG GTGTGTGAAG TATTTGAAAG TGATTGTGGA
    AACAACAAAA ATGAACAGAA AGATGCAAAA ACAGGAGTCT GCATCACTGC TCTAAAAACA
    GAAATAAAAA ACATAGATAC TGTGAGTACT ACTCAAGAAA AGCCACATTG TCAAAGAGAC
    ACAGGAATAT CTTCCTCCCT AATGGGTAAG AATGTTCAGA GGGAACTCAG AAGGGGAGGA
    AGGAGAAACT GGACCCATTC TGATGCATTT CGAGTCTCCT TGGATCAAGA TGTGGAGACT
    GAGACTGAGC CATCGGACTA CAGCAATGGT GAGGGAGCTG TTTTCAACAA GTCTCTGAGT
    GGCAGCCAGA CTTCCAGTGC TTGGAGCAAC TTATCAGGGT TTAGTTCCTC TGCAAGCTGG
    GAGGAAGTGA ATTATCACGT TGACGACAGG TCAGCCAGAA AAGAGCCTGG CAAAGAACAT
    CTGGTGGACA CTCAGTGTTC CACTGCCTTG TCTGAGGAGC TAGAGAATGA CAGGGAAGGC
    AGAGCTATGC ATTCATTGCA TTCACAGCTT CATGATCTCT CTCTTCAGGA ACCCAACAAT
    GACAATTTGG AGCCTTCTCA AAATCAGCCA CAGCAACAGA TGCCCTTGAC ACCCTTCTCG
    CCTCATAATA CCCCAGGCAT TTTCTTGGCC CCTGGTGCAG GGCTTCTAGA AGGAGCTCCA
    GAAGGTATCC AGGAAGTCAG AAATATGGGA CCCAGAAATA CTTCTGCTCA CTCCAGACCC
    TCATATCGTT CTGCTTCTTG GTCTTCTGAT TCTGGTAGGC CCAAGAATAT GGGCACACAT
    CCTTCAGTCC AAAAAGAAGA AGCCTTTGAA ATAATTGTTG AGTTTCCAGA AACCAACTGC
    GATGTCAAAG ACAGGCAGGG GAAAGAGCAG GGAGAAGAAA TTAGTGAAAG AGGCGCAGGC
    CCTACATTTA AAGCTAGTCC CTCCTGGGTT GACCCAGAAG GAGAAACAGC AGAAAGCACT
    GAAGATGCAC CCTTAGACTT TCACAGGGTC CTGCACAATT CTCTGGGAAA CATTTCCATG
    CTGCCATGTA GCTCCTTCAC CCCTAATTGG CCTGTTCAAA ATCCTGACTC CAGAAAAAGT
    GGTGGCCCAG TCGCAGAGCA GGGCATCGAC CCTGATGCCT CCACAGTGGA TGAGGAGGGG
    CAACTGCTCG ACAGCATGGA TGTTCCCTGC ACAAATGGGC ACGGCTCTCA TAGACTGTGC
    ATTCTGAGAC AGCCGCCTGG TCAGAGGGCG GAGACCCCCA ATTCCTCTGT AAGCGGTAAC
    ATCCTCTTCC CTGTCCTCAG CGAGGACTGC ACTACCACAG AGGAAGGAAA TCAGCCTGGA
    AACATGCTAA ACTGCAGCCA GAACTCCAGC TCATCCTCAG TGTGGTGGCT GAAATCACCT
    GCATTTTCCA GTGGTTCTTC TGAGGGGGAC AGCCCTTGGT CCTATCTGAA TTCCAGTGGG
    AGTTCTTGGG TTTCATTGCC GGGAAAGATG AGGAAAGAGA TCCTTGAGGC TCGCACCTTG
    CAACCTGATG ACTTTGAAAA GCTGTTGGCA GGAGTGAGGC ATGATTGGCT GTTTCAGAGA
    CTAGAGAATA CGGGGGTTTT TAAGCCCAGT CAACTCCACC GAGCACATAG TGCTCTTTTG
    TTAAAATATT CAAAAAAATC TGAACTGTGG ACGGCCCAGG AAACTATTGT CTATTTGGGG
    GACTACTTGA CTGTGAAGAA AAAAGGCAGA CAAAGAAATG CTTTTTGGGT TCATCATCTT
    CATCAAGAAG AAATTCTGGG GAGGTATGTT GGGAAAGACT ATAAGGAGCA GAAGGGGCTC
    TGGCACCACT TCACTGATGT GGAGCGACAG ATGACCGCAC AGCACTATGT GACAGAATTT
    AACAAGAGAC TCTATGAACA AAACATTCCC ACCCAGATAT TCTACATCCC ATCCACAATA
    CTACTGATTT TAGAGGACAA GACAATAAAG GGATGTATCA GTGTGGAGCC TTACATACTG
    GGAGAATTTG TAAAATTGTC AAATAACACG AAAGTGGTGA AAACAGAATA CAAAGCCACA
    GAATATGGCT TGGCCTATGG CCATTTTTCT TATGAGTTTT CTAATCATAG AGATGTTGTG
    GTCGATTTAC AAGGTTGGGT AACCGGTAAT GGAAAAGGAC TCATCTACCT CACAGATCCC
    CAGATTCACT CCGTTGATCA GAAAGTTTTC ACTACCAATT TTGGAAAGAG AGGAATTTTT
    TACTTCTTTA ATAACCAGCA TGTGGAATGT AATGAAATCT GCCATCGTCT TTCTTTGACT
    AGACCTTCAA TGGAGAAACC ATGTAAGTCA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864135.1
    CDS280..3912
    Translation

    Target ORF information:

    RefSeq Version XM_017008646.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008646.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGAATAT CCGTGGCACG TGTTTATTGT CCTACAGTAG TTCAAATGAC
    TGTCCTCCAG AATTGAAAAA CTTACATCTG TGTGAAGCCA AAGAGGCCTT TGAGATTGGC
    CTCCTCACCA AGAGAGATGA TGAGCCTGTT ACTGGAAAAC AGGAGCTTCA CAGCTTTGTC
    AAAGCTGCTT TCGGTCTCAC CACAGTGCAC AGAAGGCTCC ATGGGGAGAC AGGGACGGTC
    CATGCAGCAA GTCAGCTCTG TAAGGAAGCA ATGGGGAAGC TGTACAATTT CAGCACTTCC
    TCCAGAAGTC AGGACAGAGA AGCTCTGTCT CAAGAAGTTA TGTCTGTGAT TGCCCAGGTG
    AAGGAACATT TACAAGTTCA AAGCTTCTCA AATGTAGATG ACAGATCTTA TGTTCCCGAG
    AGTTTCGAGT GCAGGTTGGA TAAACTTATC TTGCATGGGC AAGGGGATTT CCAAAAAATC
    CTTGACACCT ATTCACAGCA CCATACTTCG GTGTGTGAAG TATTTGAAAG TGATTGTGGA
    AACAACAAAA ATGAACAGAA AGATGCAAAA ACAGGAGTCT GCATCACTGC TCTAAAAACA
    GAAATAAAAA ACATAGATAC TGTGAGTACT ACTCAAGAAA AGCCACATTG TCAAAGAGAC
    ACAGGAATAT CTTCCTCCCT AATGGGTAAG AATGTTCAGA GGGAACTCAG AAGGGGAGGA
    AGGAGAAACT GGACCCATTC TGATGCATTT CGAGTCTCCT TGGATCAAGA TGTGGAGACT
    GAGACTGAGC CATCGGACTA CAGCAATGGT GAGGGAGCTG TTTTCAACAA GTCTCTGAGT
    GGCAGCCAGA CTTCCAGTGC TTGGAGCAAC TTATCAGGGT TTAGTTCCTC TGCAAGCTGG
    GAGGAAGTGA ATTATCACGT TGACGACAGG TCAGCCAGAA AAGAGCCTGG CAAAGAACAT
    CTGGTGGACA CTCAGTGTTC CACTGCCTTG TCTGAGGAGC TAGAGAATGA CAGGGAAGGC
    AGAGCTATGC ATTCATTGCA TTCACAGCTT CATGATCTCT CTCTTCAGGA ACCCAACAAT
    GACAATTTGG AGCCTTCTCA AAATCAGCCA CAGCAACAGA TGCCCTTGAC ACCCTTCTCG
    CCTCATAATA CCCCAGGCAT TTTCTTGGCC CCTGGTGCAG GGCTTCTAGA AGGAGCTCCA
    GAAGGTATCC AGGAAGTCAG AAATATGGGA CCCAGAAATA CTTCTGCTCA CTCCAGACCC
    TCATATCGTT CTGCTTCTTG GTCTTCTGAT TCTGGTAGGC CCAAGAATAT GGGCACACAT
    CCTTCAGTCC AAAAAGAAGA AGCCTTTGAA ATAATTGTTG AGTTTCCAGA AACCAACTGC
    GATGTCAAAG ACAGGCAGGG GAAAGAGCAG GGAGAAGAAA TTAGTGAAAG AGGCGCAGGC
    CCTACATTTA AAGCTAGTCC CTCCTGGGTT GACCCAGAAG GAGAAACAGC AGAAAGCACT
    GAAGATGCAC CCTTAGACTT TCACAGGGTC CTGCACAATT CTCTGGGAAA CATTTCCATG
    CTGCCATGTA GCTCCTTCAC CCCTAATTGG CCTGTTCAAA ATCCTGACTC CAGAAAAAGT
    GGTGGCCCAG TCGCAGAGCA GGGCATCGAC CCTGATGCCT CCACAGTGGA TGAGGAGGGG
    CAACTGCTCG ACAGCATGGA TGTTCCCTGC ACAAATGGGC ACGGCTCTCA TAGACTGTGC
    ATTCTGAGAC AGCCGCCTGG TCAGAGGGCG GAGACCCCCA ATTCCTCTGT AAGCGGTAAC
    ATCCTCTTCC CTGTCCTCAG CGAGGACTGC ACTACCACAG AGGAAGGAAA TCAGCCTGGA
    AACATGCTAA ACTGCAGCCA GAACTCCAGC TCATCCTCAG TGTGGTGGCT GAAATCACCT
    GCATTTTCCA GTGGTTCTTC TGAGGGGGAC AGCCCTTGGT CCTATCTGAA TTCCAGTGGG
    AGTTCTTGGG TTTCATTGCC GGGAAAGATG AGGAAAGAGA TCCTTGAGGC TCGCACCTTG
    CAACCTGATG ACTTTGAAAA GCTGTTGGCA GGAGTGAGGC ATGATTGGCT GTTTCAGAGA
    CTAGAGAATA CGGGGGTTTT TAAGCCCAGT CAACTCCACC GAGCACATAG TGCTCTTTTG
    TTAAAATATT CAAAAAAATC TGAACTGTGG ACGGCCCAGG AAACTATTGT CTATTTGGGG
    GACTACTTGA CTGTGAAGAA AAAAGGCAGA CAAAGAAATG CTTTTTGGGT TCATCATCTT
    CATCAAGAAG AAATTCTGGG GAGGTATGTT GGGAAAGACT ATAAGGAGCA GAAGGGGCTC
    TGGCACCACT TCACTGATGT GGAGCGACAG ATGACCGCAC AGCACTATGT GACAGAATTT
    AACAAGAGAC TCTATGAACA AAACATTCCC ACCCAGATAT TCTACATCCC ATCCACAATA
    CTACTGATTT TAGAGGACAA GACAATAAAG GGATGTATCA GTGTGGAGCC TTACATACTG
    GGAGAATTTG TAAAATTGTC AAATAACACG AAAGTGGTGA AAACAGAATA CAAAGCCACA
    GAATATGGCT TGGCCTATGG CCATTTTTCT TATGAGTTTT CTAATCATAG AGATGTTGTG
    GTCGATTTAC AAGGTTGGGT AACCGGTAAT GGAAAAGGAC TCATCTACCT CACAGATCCC
    CAGATTCACT CCGTTGATCA GAAAGTTTTC ACTACCAATT TTGGAAAGAG AGGAATTTTT
    TACTTCTTTA ATAACCAGCA TGTGGAATGT AATGAAATCT GCCATCGTCT TTCTTTGACT
    AGACCTTCAA TGGAGAAACC ATGTAAGTCA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95417
    Clone ID Related Accession (Same CDS sequence) XM_017008647.1 , XM_017008648.1
    Accession Version XM_017008647.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3522bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTGTCGGA AGAGGACAAG AGCGAGGACC AGCGCTGCAG AGGCGTCCCT GAGGGCCTCC 
    ATCCTCGCTC GGGACTGTGC GGCTGCGGCG GCTATTGTGT TCTTGGTGGA CCGGTTCCTG
    TATGGGCTCG ACGTCTCTGG AAAACTTCTG CAGGTCGCCA AAGGTCTCCA CAAGTTGCAG
    CCAGCCACGC CAATTGCCCC GCAGGTGGTT ATTCGCCAAG CCCGAATCTC CGTGAACTCA
    GGAAAACTTT TAAAAGCAGA GTATATTCTG AGCAGTCTAA TAAGCAACAA TGGAGCAACG
    GGTACCTGGC TGTACAGAAA TGAAAGTGAC AAGGTCCTGG TGCAGTCGGT CTGTATACAG
    ATCAGAGGGC AGATTCTGCA AAAGCTGGGG ATGTGGTACG AAGCAGCAGA GTTAATATGG
    GCCTCCATTG TAGGATATTT GGCACTTCCT CAGCCGGATA AAAAGGGCCT CTCCACGTCG
    CTAGGTATAC TGGCAGACAT CTTTGTTTCC ATGAGCAAGA ACGATTATGA AAAGTTTAAA
    AACAATCCAC AAATTAATTT GCCTCCTGTT TTCTCACAGA GCCTGCTGAA GGAGTTTGAC
    CACCATTTGC TGTCCGCTGC AGAAGCCTGC AAGCTGGCAG CTGCCTTCAG TGCCTATACG
    CCGCTCTTCG TGCTCACAGC TGTGAATATC CGTGGCACGT GTTTATTGTC CTACAGTAGT
    TCAAATGACT GTCCTCCAGA ATTGAAAAAC TTACATCTGT GTGAAGCCAA AGAGGCCTTT
    GAGATTGGCC TCCTCACCAA GAGAGATGAT GAGCCTGTTA CTGGAAAACA GGAGCTTCAC
    AGCTTTGTCA AAGCTGCTTT CGGTCTCACC ACAGTGCACA GAAGGCTCCA TGGGGAGACA
    GGGACGGTCC ATGCAGCAAG TCAGCTCTGT AAGGAAGCAA TGGGGAAGCT GTACAATTTC
    AGCACTTCCT CCAGAAGTCA GGACAGAGAA GCTCTGTCTC AAGAAGTTAT GTCTGTGATT
    GCCCAGGTGA AGGAACATTT ACAAGTTCAA AGCTTCTCAA ATGTAGATGA CAGATCTTAT
    GTTCCCGAGA GTTTCGAGTG CAGGTTGGAT AAACTTATCT TGCATGGGCA AGGGGATTTC
    CAAAAAATCC TTGACACCTA TTCACAGCAC CATACTTCGG TGTGTGAAGT ATTTGAAAGT
    GATTGTGGAA ACAACAAAAA TGAACAGAAA GATGCAAAAA CAGGAGTCTG CATCACTGCT
    CTAAAAACAG AAATAAAAAA CATAGATACT GTGAGTACTA CTCAAGAAAA GCCACATTGT
    CAAAGAGACA CAGGAATATC TTCCTCCCTA ATGGGTAAGA ATGTTCAGAG GGAACTCAGA
    AGGGGAGGAA GGAGAAACTG GACCCATTCT GATGCATTTC GAGTCTCCTT GGATCAAGAT
    GTGGAGACTG AGACTGAGCC ATCGGACTAC AGCAATGGTG AGGGAGCTGT TTTCAACAAG
    TCTCTGAGTG GCAGCCAGAC TTCCAGTGCT TGGAGCAACT TATCAGGGTT TAGTTCCTCT
    GCAAGCTGGG AGGAAGTGAA TTATCACGTT GACGACAGGT CAGCCAGAAA AGAGCCTGGC
    AAAGAACATC TGGTGGACAC TCAGTGTTCC ACTGCCTTGT CTGAGGAGCT AGAGAATGAC
    AGGGAAGGCA GAGCTATGCA TTCATTGCAT TCACAGCTTC ATGATCTCTC TCTTCAGGAA
    CCCAACAATG ACAATTTGGA GCCTTCTCAA AATCAGCCAC AGCAACAGAT GCCCTTGACA
    CCCTTCTCGC CTCATAATAC CCCAGGCATT TTCTTGGCCC CTGGTGCAGG GCTTCTAGAA
    GGAGCTCCAG AAGGTATCCA GGAAGTCAGA AATATGGGAC CCAGAAATAC TTCTGCTCAC
    TCCAGACCCT CATATCGTTC TGCTTCTTGG TCTTCTGATT CTGGTAGGCC CAAGAATATG
    GGCACACATC CTTCAGTCCA AAAAGAAGAA GCCTTTGAAA TAATTGTTGA GTTTCCAGAA
    ACCAACTGCG ATGTCAAAGA CAGGCAGGGG AAAGAGCAGG GAGAAGAAAT TAGTGAAAGA
    GGCGCAGGCC CTACATTTAA AGCTAGTCCC TCCTGGGTTG ACCCAGAAGG AGAAACAGCA
    GAAAGCACTG AAGATGCACC CTTAGACTTT CACAGGGTCC TGCACAATTC TCTGGGAAAC
    ATTTCCATGC TGCCATGTAG CTCCTTCACC CCTAATTGGC CTGTTCAAAA TCCTGACTCC
    AGAAAAAGTG GTGGCCCAGT CGCAGAGCAG GGCATCGACC CTGATGCCTC CACAGTGGAT
    GAGGAGGGGC AACTGCTCGA CAGCATGGAT GTTCCCTGCA CAAATGGGCA CGGCTCTCAT
    AGACTGTGCA TTCTGAGACA GCCGCCTGGT CAGAGGGCGG AGACCCCCAA TTCCTCTGTA
    AGCGGTAACA TCCTCTTCCC TGTCCTCAGC GAGGACTGCA CTACCACAGA GGAAGGAAAT
    CAGCCTGGAA ACATGCTAAA CTGCAGCCAG AACTCCAGCT CATCCTCAGT GTGGTGGCTG
    AAATCACCTG CATTTTCCAG TGGTTCTTCT GAGGGGGACA GCCCTTGGTC CTATCTGAAT
    TCCAGTGGGA GTTCTTGGGT TTCATTGCCG GGAAAGATGA GGAAAGAGAT CCTTGAGGCT
    CGCACCTTGC AACCTGATGA CTTTGAAAAG CTGTTGGCAG GAGTGAGGCA TGATTGGCTG
    TTTCAGAGAC TAGAGAATAC GGGGGTTTTT AAGCCCAGTC AACTCCACCG AGCACATAGT
    GCTCTTTTGT TAAAATATTC AAAAAAATCT GAACTGTGGA CGGCCCAGGA AACTATTGTC
    TATTTGGGGG ACTACTTGAC TGTGAAGAAA AAAGGCAGAC AAAGAAATGC TTTTTGGGTT
    CATCATCTTC ATCAAGAAGA AATTCTGGGG AGGTATGTTG GGAAAGACTA TAAGGAGCAG
    AAGGGGCTCT GGCACCACTT CACTGATGTG GAGCGACAGA TGACCGCACA GCACTATGTG
    ACAGAATTTA ACAAGAGACT CTATGAACAA AACATTCCCA CCCAGATATT CTACATCCCA
    TCCACAATAC TACTGATTTT AGAGGACAAG ACAATAAAGG GATGTATCAG TGTGGAGCCT
    TACATACTGG GAGAATTTGT AAAATTGTCA AATAACACGA AAGTGGTGAA AACAGAATAC
    AAAGCCACAG AATATGGCTT GGCCTATGGC CATTTTTCTT ATGAGTTTTC TAATCATAGA
    GATGTTGTGG TCGATTTACA AGGTTGGGTA ACCGGTAATG GAAAAGGACT CATCTACCTC
    ACAGATCCCC AGATTCACTC CGTTGATCAG AAAGTTTTCA CTACCAATTT TGGAAAGAGA
    GGAATTTTTT ACTTCTTTAA TAACCAGCAT GTGGAATGTA ATGAAATCTG CCATCGTCTT
    TCTTTGACTA GACCTTCAAT GGAGAAACCA TGTAAGTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864136.1
    CDS365..3886
    Translation

    Target ORF information:

    RefSeq Version XM_017008647.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008647.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTGTCGGA AGAGGACAAG AGCGAGGACC AGCGCTGCAG AGGCGTCCCT GAGGGCCTCC 
    ATCCTCGCTC GGGACTGTGC GGCTGCGGCG GCTATTGTGT TCTTGGTGGA CCGGTTCCTG
    TATGGGCTCG ACGTCTCTGG AAAACTTCTG CAGGTCGCCA AAGGTCTCCA CAAGTTGCAG
    CCAGCCACGC CAATTGCCCC GCAGGTGGTT ATTCGCCAAG CCCGAATCTC CGTGAACTCA
    GGAAAACTTT TAAAAGCAGA GTATATTCTG AGCAGTCTAA TAAGCAACAA TGGAGCAACG
    GGTACCTGGC TGTACAGAAA TGAAAGTGAC AAGGTCCTGG TGCAGTCGGT CTGTATACAG
    ATCAGAGGGC AGATTCTGCA AAAGCTGGGG ATGTGGTACG AAGCAGCAGA GTTAATATGG
    GCCTCCATTG TAGGATATTT GGCACTTCCT CAGCCGGATA AAAAGGGCCT CTCCACGTCG
    CTAGGTATAC TGGCAGACAT CTTTGTTTCC ATGAGCAAGA ACGATTATGA AAAGTTTAAA
    AACAATCCAC AAATTAATTT GCCTCCTGTT TTCTCACAGA GCCTGCTGAA GGAGTTTGAC
    CACCATTTGC TGTCCGCTGC AGAAGCCTGC AAGCTGGCAG CTGCCTTCAG TGCCTATACG
    CCGCTCTTCG TGCTCACAGC TGTGAATATC CGTGGCACGT GTTTATTGTC CTACAGTAGT
    TCAAATGACT GTCCTCCAGA ATTGAAAAAC TTACATCTGT GTGAAGCCAA AGAGGCCTTT
    GAGATTGGCC TCCTCACCAA GAGAGATGAT GAGCCTGTTA CTGGAAAACA GGAGCTTCAC
    AGCTTTGTCA AAGCTGCTTT CGGTCTCACC ACAGTGCACA GAAGGCTCCA TGGGGAGACA
    GGGACGGTCC ATGCAGCAAG TCAGCTCTGT AAGGAAGCAA TGGGGAAGCT GTACAATTTC
    AGCACTTCCT CCAGAAGTCA GGACAGAGAA GCTCTGTCTC AAGAAGTTAT GTCTGTGATT
    GCCCAGGTGA AGGAACATTT ACAAGTTCAA AGCTTCTCAA ATGTAGATGA CAGATCTTAT
    GTTCCCGAGA GTTTCGAGTG CAGGTTGGAT AAACTTATCT TGCATGGGCA AGGGGATTTC
    CAAAAAATCC TTGACACCTA TTCACAGCAC CATACTTCGG TGTGTGAAGT ATTTGAAAGT
    GATTGTGGAA ACAACAAAAA TGAACAGAAA GATGCAAAAA CAGGAGTCTG CATCACTGCT
    CTAAAAACAG AAATAAAAAA CATAGATACT GTGAGTACTA CTCAAGAAAA GCCACATTGT
    CAAAGAGACA CAGGAATATC TTCCTCCCTA ATGGGTAAGA ATGTTCAGAG GGAACTCAGA
    AGGGGAGGAA GGAGAAACTG GACCCATTCT GATGCATTTC GAGTCTCCTT GGATCAAGAT
    GTGGAGACTG AGACTGAGCC ATCGGACTAC AGCAATGGTG AGGGAGCTGT TTTCAACAAG
    TCTCTGAGTG GCAGCCAGAC TTCCAGTGCT TGGAGCAACT TATCAGGGTT TAGTTCCTCT
    GCAAGCTGGG AGGAAGTGAA TTATCACGTT GACGACAGGT CAGCCAGAAA AGAGCCTGGC
    AAAGAACATC TGGTGGACAC TCAGTGTTCC ACTGCCTTGT CTGAGGAGCT AGAGAATGAC
    AGGGAAGGCA GAGCTATGCA TTCATTGCAT TCACAGCTTC ATGATCTCTC TCTTCAGGAA
    CCCAACAATG ACAATTTGGA GCCTTCTCAA AATCAGCCAC AGCAACAGAT GCCCTTGACA
    CCCTTCTCGC CTCATAATAC CCCAGGCATT TTCTTGGCCC CTGGTGCAGG GCTTCTAGAA
    GGAGCTCCAG AAGGTATCCA GGAAGTCAGA AATATGGGAC CCAGAAATAC TTCTGCTCAC
    TCCAGACCCT CATATCGTTC TGCTTCTTGG TCTTCTGATT CTGGTAGGCC CAAGAATATG
    GGCACACATC CTTCAGTCCA AAAAGAAGAA GCCTTTGAAA TAATTGTTGA GTTTCCAGAA
    ACCAACTGCG ATGTCAAAGA CAGGCAGGGG AAAGAGCAGG GAGAAGAAAT TAGTGAAAGA
    GGCGCAGGCC CTACATTTAA AGCTAGTCCC TCCTGGGTTG ACCCAGAAGG AGAAACAGCA
    GAAAGCACTG AAGATGCACC CTTAGACTTT CACAGGGTCC TGCACAATTC TCTGGGAAAC
    ATTTCCATGC TGCCATGTAG CTCCTTCACC CCTAATTGGC CTGTTCAAAA TCCTGACTCC
    AGAAAAAGTG GTGGCCCAGT CGCAGAGCAG GGCATCGACC CTGATGCCTC CACAGTGGAT
    GAGGAGGGGC AACTGCTCGA CAGCATGGAT GTTCCCTGCA CAAATGGGCA CGGCTCTCAT
    AGACTGTGCA TTCTGAGACA GCCGCCTGGT CAGAGGGCGG AGACCCCCAA TTCCTCTGTA
    AGCGGTAACA TCCTCTTCCC TGTCCTCAGC GAGGACTGCA CTACCACAGA GGAAGGAAAT
    CAGCCTGGAA ACATGCTAAA CTGCAGCCAG AACTCCAGCT CATCCTCAGT GTGGTGGCTG
    AAATCACCTG CATTTTCCAG TGGTTCTTCT GAGGGGGACA GCCCTTGGTC CTATCTGAAT
    TCCAGTGGGA GTTCTTGGGT TTCATTGCCG GGAAAGATGA GGAAAGAGAT CCTTGAGGCT
    CGCACCTTGC AACCTGATGA CTTTGAAAAG CTGTTGGCAG GAGTGAGGCA TGATTGGCTG
    TTTCAGAGAC TAGAGAATAC GGGGGTTTTT AAGCCCAGTC AACTCCACCG AGCACATAGT
    GCTCTTTTGT TAAAATATTC AAAAAAATCT GAACTGTGGA CGGCCCAGGA AACTATTGTC
    TATTTGGGGG ACTACTTGAC TGTGAAGAAA AAAGGCAGAC AAAGAAATGC TTTTTGGGTT
    CATCATCTTC ATCAAGAAGA AATTCTGGGG AGGTATGTTG GGAAAGACTA TAAGGAGCAG
    AAGGGGCTCT GGCACCACTT CACTGATGTG GAGCGACAGA TGACCGCACA GCACTATGTG
    ACAGAATTTA ACAAGAGACT CTATGAACAA AACATTCCCA CCCAGATATT CTACATCCCA
    TCCACAATAC TACTGATTTT AGAGGACAAG ACAATAAAGG GATGTATCAG TGTGGAGCCT
    TACATACTGG GAGAATTTGT AAAATTGTCA AATAACACGA AAGTGGTGAA AACAGAATAC
    AAAGCCACAG AATATGGCTT GGCCTATGGC CATTTTTCTT ATGAGTTTTC TAATCATAGA
    GATGTTGTGG TCGATTTACA AGGTTGGGTA ACCGGTAATG GAAAAGGACT CATCTACCTC
    ACAGATCCCC AGATTCACTC CGTTGATCAG AAAGTTTTCA CTACCAATTT TGGAAAGAGA
    GGAATTTTTT ACTTCTTTAA TAACCAGCAT GTGGAATGTA ATGAAATCTG CCATCGTCTT
    TCTTTGACTA GACCTTCAAT GGAGAAACCA TGTAAGTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95417
    Clone ID Related Accession (Same CDS sequence) XM_017008647.1 , XM_017008648.1
    Accession Version XM_017008648.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3522bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTGTCGGA AGAGGACAAG AGCGAGGACC AGCGCTGCAG AGGCGTCCCT GAGGGCCTCC 
    ATCCTCGCTC GGGACTGTGC GGCTGCGGCG GCTATTGTGT TCTTGGTGGA CCGGTTCCTG
    TATGGGCTCG ACGTCTCTGG AAAACTTCTG CAGGTCGCCA AAGGTCTCCA CAAGTTGCAG
    CCAGCCACGC CAATTGCCCC GCAGGTGGTT ATTCGCCAAG CCCGAATCTC CGTGAACTCA
    GGAAAACTTT TAAAAGCAGA GTATATTCTG AGCAGTCTAA TAAGCAACAA TGGAGCAACG
    GGTACCTGGC TGTACAGAAA TGAAAGTGAC AAGGTCCTGG TGCAGTCGGT CTGTATACAG
    ATCAGAGGGC AGATTCTGCA AAAGCTGGGG ATGTGGTACG AAGCAGCAGA GTTAATATGG
    GCCTCCATTG TAGGATATTT GGCACTTCCT CAGCCGGATA AAAAGGGCCT CTCCACGTCG
    CTAGGTATAC TGGCAGACAT CTTTGTTTCC ATGAGCAAGA ACGATTATGA AAAGTTTAAA
    AACAATCCAC AAATTAATTT GCCTCCTGTT TTCTCACAGA GCCTGCTGAA GGAGTTTGAC
    CACCATTTGC TGTCCGCTGC AGAAGCCTGC AAGCTGGCAG CTGCCTTCAG TGCCTATACG
    CCGCTCTTCG TGCTCACAGC TGTGAATATC CGTGGCACGT GTTTATTGTC CTACAGTAGT
    TCAAATGACT GTCCTCCAGA ATTGAAAAAC TTACATCTGT GTGAAGCCAA AGAGGCCTTT
    GAGATTGGCC TCCTCACCAA GAGAGATGAT GAGCCTGTTA CTGGAAAACA GGAGCTTCAC
    AGCTTTGTCA AAGCTGCTTT CGGTCTCACC ACAGTGCACA GAAGGCTCCA TGGGGAGACA
    GGGACGGTCC ATGCAGCAAG TCAGCTCTGT AAGGAAGCAA TGGGGAAGCT GTACAATTTC
    AGCACTTCCT CCAGAAGTCA GGACAGAGAA GCTCTGTCTC AAGAAGTTAT GTCTGTGATT
    GCCCAGGTGA AGGAACATTT ACAAGTTCAA AGCTTCTCAA ATGTAGATGA CAGATCTTAT
    GTTCCCGAGA GTTTCGAGTG CAGGTTGGAT AAACTTATCT TGCATGGGCA AGGGGATTTC
    CAAAAAATCC TTGACACCTA TTCACAGCAC CATACTTCGG TGTGTGAAGT ATTTGAAAGT
    GATTGTGGAA ACAACAAAAA TGAACAGAAA GATGCAAAAA CAGGAGTCTG CATCACTGCT
    CTAAAAACAG AAATAAAAAA CATAGATACT GTGAGTACTA CTCAAGAAAA GCCACATTGT
    CAAAGAGACA CAGGAATATC TTCCTCCCTA ATGGGTAAGA ATGTTCAGAG GGAACTCAGA
    AGGGGAGGAA GGAGAAACTG GACCCATTCT GATGCATTTC GAGTCTCCTT GGATCAAGAT
    GTGGAGACTG AGACTGAGCC ATCGGACTAC AGCAATGGTG AGGGAGCTGT TTTCAACAAG
    TCTCTGAGTG GCAGCCAGAC TTCCAGTGCT TGGAGCAACT TATCAGGGTT TAGTTCCTCT
    GCAAGCTGGG AGGAAGTGAA TTATCACGTT GACGACAGGT CAGCCAGAAA AGAGCCTGGC
    AAAGAACATC TGGTGGACAC TCAGTGTTCC ACTGCCTTGT CTGAGGAGCT AGAGAATGAC
    AGGGAAGGCA GAGCTATGCA TTCATTGCAT TCACAGCTTC ATGATCTCTC TCTTCAGGAA
    CCCAACAATG ACAATTTGGA GCCTTCTCAA AATCAGCCAC AGCAACAGAT GCCCTTGACA
    CCCTTCTCGC CTCATAATAC CCCAGGCATT TTCTTGGCCC CTGGTGCAGG GCTTCTAGAA
    GGAGCTCCAG AAGGTATCCA GGAAGTCAGA AATATGGGAC CCAGAAATAC TTCTGCTCAC
    TCCAGACCCT CATATCGTTC TGCTTCTTGG TCTTCTGATT CTGGTAGGCC CAAGAATATG
    GGCACACATC CTTCAGTCCA AAAAGAAGAA GCCTTTGAAA TAATTGTTGA GTTTCCAGAA
    ACCAACTGCG ATGTCAAAGA CAGGCAGGGG AAAGAGCAGG GAGAAGAAAT TAGTGAAAGA
    GGCGCAGGCC CTACATTTAA AGCTAGTCCC TCCTGGGTTG ACCCAGAAGG AGAAACAGCA
    GAAAGCACTG AAGATGCACC CTTAGACTTT CACAGGGTCC TGCACAATTC TCTGGGAAAC
    ATTTCCATGC TGCCATGTAG CTCCTTCACC CCTAATTGGC CTGTTCAAAA TCCTGACTCC
    AGAAAAAGTG GTGGCCCAGT CGCAGAGCAG GGCATCGACC CTGATGCCTC CACAGTGGAT
    GAGGAGGGGC AACTGCTCGA CAGCATGGAT GTTCCCTGCA CAAATGGGCA CGGCTCTCAT
    AGACTGTGCA TTCTGAGACA GCCGCCTGGT CAGAGGGCGG AGACCCCCAA TTCCTCTGTA
    AGCGGTAACA TCCTCTTCCC TGTCCTCAGC GAGGACTGCA CTACCACAGA GGAAGGAAAT
    CAGCCTGGAA ACATGCTAAA CTGCAGCCAG AACTCCAGCT CATCCTCAGT GTGGTGGCTG
    AAATCACCTG CATTTTCCAG TGGTTCTTCT GAGGGGGACA GCCCTTGGTC CTATCTGAAT
    TCCAGTGGGA GTTCTTGGGT TTCATTGCCG GGAAAGATGA GGAAAGAGAT CCTTGAGGCT
    CGCACCTTGC AACCTGATGA CTTTGAAAAG CTGTTGGCAG GAGTGAGGCA TGATTGGCTG
    TTTCAGAGAC TAGAGAATAC GGGGGTTTTT AAGCCCAGTC AACTCCACCG AGCACATAGT
    GCTCTTTTGT TAAAATATTC AAAAAAATCT GAACTGTGGA CGGCCCAGGA AACTATTGTC
    TATTTGGGGG ACTACTTGAC TGTGAAGAAA AAAGGCAGAC AAAGAAATGC TTTTTGGGTT
    CATCATCTTC ATCAAGAAGA AATTCTGGGG AGGTATGTTG GGAAAGACTA TAAGGAGCAG
    AAGGGGCTCT GGCACCACTT CACTGATGTG GAGCGACAGA TGACCGCACA GCACTATGTG
    ACAGAATTTA ACAAGAGACT CTATGAACAA AACATTCCCA CCCAGATATT CTACATCCCA
    TCCACAATAC TACTGATTTT AGAGGACAAG ACAATAAAGG GATGTATCAG TGTGGAGCCT
    TACATACTGG GAGAATTTGT AAAATTGTCA AATAACACGA AAGTGGTGAA AACAGAATAC
    AAAGCCACAG AATATGGCTT GGCCTATGGC CATTTTTCTT ATGAGTTTTC TAATCATAGA
    GATGTTGTGG TCGATTTACA AGGTTGGGTA ACCGGTAATG GAAAAGGACT CATCTACCTC
    ACAGATCCCC AGATTCACTC CGTTGATCAG AAAGTTTTCA CTACCAATTT TGGAAAGAGA
    GGAATTTTTT ACTTCTTTAA TAACCAGCAT GTGGAATGTA ATGAAATCTG CCATCGTCTT
    TCTTTGACTA GACCTTCAAT GGAGAAACCA TGTAAGTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864137.1
    CDS313..3834
    Translation

    Target ORF information:

    RefSeq Version XM_017008648.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008648.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTGTCGGA AGAGGACAAG AGCGAGGACC AGCGCTGCAG AGGCGTCCCT GAGGGCCTCC 
    ATCCTCGCTC GGGACTGTGC GGCTGCGGCG GCTATTGTGT TCTTGGTGGA CCGGTTCCTG
    TATGGGCTCG ACGTCTCTGG AAAACTTCTG CAGGTCGCCA AAGGTCTCCA CAAGTTGCAG
    CCAGCCACGC CAATTGCCCC GCAGGTGGTT ATTCGCCAAG CCCGAATCTC CGTGAACTCA
    GGAAAACTTT TAAAAGCAGA GTATATTCTG AGCAGTCTAA TAAGCAACAA TGGAGCAACG
    GGTACCTGGC TGTACAGAAA TGAAAGTGAC AAGGTCCTGG TGCAGTCGGT CTGTATACAG
    ATCAGAGGGC AGATTCTGCA AAAGCTGGGG ATGTGGTACG AAGCAGCAGA GTTAATATGG
    GCCTCCATTG TAGGATATTT GGCACTTCCT CAGCCGGATA AAAAGGGCCT CTCCACGTCG
    CTAGGTATAC TGGCAGACAT CTTTGTTTCC ATGAGCAAGA ACGATTATGA AAAGTTTAAA
    AACAATCCAC AAATTAATTT GCCTCCTGTT TTCTCACAGA GCCTGCTGAA GGAGTTTGAC
    CACCATTTGC TGTCCGCTGC AGAAGCCTGC AAGCTGGCAG CTGCCTTCAG TGCCTATACG
    CCGCTCTTCG TGCTCACAGC TGTGAATATC CGTGGCACGT GTTTATTGTC CTACAGTAGT
    TCAAATGACT GTCCTCCAGA ATTGAAAAAC TTACATCTGT GTGAAGCCAA AGAGGCCTTT
    GAGATTGGCC TCCTCACCAA GAGAGATGAT GAGCCTGTTA CTGGAAAACA GGAGCTTCAC
    AGCTTTGTCA AAGCTGCTTT CGGTCTCACC ACAGTGCACA GAAGGCTCCA TGGGGAGACA
    GGGACGGTCC ATGCAGCAAG TCAGCTCTGT AAGGAAGCAA TGGGGAAGCT GTACAATTTC
    AGCACTTCCT CCAGAAGTCA GGACAGAGAA GCTCTGTCTC AAGAAGTTAT GTCTGTGATT
    GCCCAGGTGA AGGAACATTT ACAAGTTCAA AGCTTCTCAA ATGTAGATGA CAGATCTTAT
    GTTCCCGAGA GTTTCGAGTG CAGGTTGGAT AAACTTATCT TGCATGGGCA AGGGGATTTC
    CAAAAAATCC TTGACACCTA TTCACAGCAC CATACTTCGG TGTGTGAAGT ATTTGAAAGT
    GATTGTGGAA ACAACAAAAA TGAACAGAAA GATGCAAAAA CAGGAGTCTG CATCACTGCT
    CTAAAAACAG AAATAAAAAA CATAGATACT GTGAGTACTA CTCAAGAAAA GCCACATTGT
    CAAAGAGACA CAGGAATATC TTCCTCCCTA ATGGGTAAGA ATGTTCAGAG GGAACTCAGA
    AGGGGAGGAA GGAGAAACTG GACCCATTCT GATGCATTTC GAGTCTCCTT GGATCAAGAT
    GTGGAGACTG AGACTGAGCC ATCGGACTAC AGCAATGGTG AGGGAGCTGT TTTCAACAAG
    TCTCTGAGTG GCAGCCAGAC TTCCAGTGCT TGGAGCAACT TATCAGGGTT TAGTTCCTCT
    GCAAGCTGGG AGGAAGTGAA TTATCACGTT GACGACAGGT CAGCCAGAAA AGAGCCTGGC
    AAAGAACATC TGGTGGACAC TCAGTGTTCC ACTGCCTTGT CTGAGGAGCT AGAGAATGAC
    AGGGAAGGCA GAGCTATGCA TTCATTGCAT TCACAGCTTC ATGATCTCTC TCTTCAGGAA
    CCCAACAATG ACAATTTGGA GCCTTCTCAA AATCAGCCAC AGCAACAGAT GCCCTTGACA
    CCCTTCTCGC CTCATAATAC CCCAGGCATT TTCTTGGCCC CTGGTGCAGG GCTTCTAGAA
    GGAGCTCCAG AAGGTATCCA GGAAGTCAGA AATATGGGAC CCAGAAATAC TTCTGCTCAC
    TCCAGACCCT CATATCGTTC TGCTTCTTGG TCTTCTGATT CTGGTAGGCC CAAGAATATG
    GGCACACATC CTTCAGTCCA AAAAGAAGAA GCCTTTGAAA TAATTGTTGA GTTTCCAGAA
    ACCAACTGCG ATGTCAAAGA CAGGCAGGGG AAAGAGCAGG GAGAAGAAAT TAGTGAAAGA
    GGCGCAGGCC CTACATTTAA AGCTAGTCCC TCCTGGGTTG ACCCAGAAGG AGAAACAGCA
    GAAAGCACTG AAGATGCACC CTTAGACTTT CACAGGGTCC TGCACAATTC TCTGGGAAAC
    ATTTCCATGC TGCCATGTAG CTCCTTCACC CCTAATTGGC CTGTTCAAAA TCCTGACTCC
    AGAAAAAGTG GTGGCCCAGT CGCAGAGCAG GGCATCGACC CTGATGCCTC CACAGTGGAT
    GAGGAGGGGC AACTGCTCGA CAGCATGGAT GTTCCCTGCA CAAATGGGCA CGGCTCTCAT
    AGACTGTGCA TTCTGAGACA GCCGCCTGGT CAGAGGGCGG AGACCCCCAA TTCCTCTGTA
    AGCGGTAACA TCCTCTTCCC TGTCCTCAGC GAGGACTGCA CTACCACAGA GGAAGGAAAT
    CAGCCTGGAA ACATGCTAAA CTGCAGCCAG AACTCCAGCT CATCCTCAGT GTGGTGGCTG
    AAATCACCTG CATTTTCCAG TGGTTCTTCT GAGGGGGACA GCCCTTGGTC CTATCTGAAT
    TCCAGTGGGA GTTCTTGGGT TTCATTGCCG GGAAAGATGA GGAAAGAGAT CCTTGAGGCT
    CGCACCTTGC AACCTGATGA CTTTGAAAAG CTGTTGGCAG GAGTGAGGCA TGATTGGCTG
    TTTCAGAGAC TAGAGAATAC GGGGGTTTTT AAGCCCAGTC AACTCCACCG AGCACATAGT
    GCTCTTTTGT TAAAATATTC AAAAAAATCT GAACTGTGGA CGGCCCAGGA AACTATTGTC
    TATTTGGGGG ACTACTTGAC TGTGAAGAAA AAAGGCAGAC AAAGAAATGC TTTTTGGGTT
    CATCATCTTC ATCAAGAAGA AATTCTGGGG AGGTATGTTG GGAAAGACTA TAAGGAGCAG
    AAGGGGCTCT GGCACCACTT CACTGATGTG GAGCGACAGA TGACCGCACA GCACTATGTG
    ACAGAATTTA ACAAGAGACT CTATGAACAA AACATTCCCA CCCAGATATT CTACATCCCA
    TCCACAATAC TACTGATTTT AGAGGACAAG ACAATAAAGG GATGTATCAG TGTGGAGCCT
    TACATACTGG GAGAATTTGT AAAATTGTCA AATAACACGA AAGTGGTGAA AACAGAATAC
    AAAGCCACAG AATATGGCTT GGCCTATGGC CATTTTTCTT ATGAGTTTTC TAATCATAGA
    GATGTTGTGG TCGATTTACA AGGTTGGGTA ACCGGTAATG GAAAAGGACT CATCTACCTC
    ACAGATCCCC AGATTCACTC CGTTGATCAG AAAGTTTTCA CTACCAATTT TGGAAAGAGA
    GGAATTTTTT ACTTCTTTAA TAACCAGCAT GTGGAATGTA ATGAAATCTG CCATCGTCTT
    TCTTTGACTA GACCTTCAAT GGAGAAACCA TGTAAGTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95418
    Clone ID Related Accession (Same CDS sequence) XM_017008649.1 , XM_017008649.2
    Accession Version XM_017008649.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3132bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTGGTACG AAGCAGCAGA GTTAATATGG GCCTCCATTG TAGGATATTT GGCACTTCCT 
    CAGCCGGATA AAAAGGGCCT CTCCACGTCG CTAGGTATAC TGGCAGACAT CTTTGTTTCC
    ATGAGCAAGA ACGATTATGA AAAGTTTAAA AACAATCCAC AAATTAATTT GCCTCCTGTT
    TTCTCACAGA GCCTGCTGAA GGAGTTTGAC CACCATTTGC TGTCCGCTGC AGAAGCCTGC
    AAGCTGGCAG CTGCCTTCAG TGCCTATACG CCGCTCTTCG TGCTCACAGC TGTGAATATC
    CGTGGCACGT GTTTATTGTC CTACAGTAGT TCAAATGACT GTCCTCCAGA ATTGAAAAAC
    TTACATCTGT GTGAAGCCAA AGAGGCCTTT GAGATTGGCC TCCTCACCAA GAGAGATGAT
    GAGCCTGTTA CTGGAAAACA GGAGCTTCAC AGCTTTGTCA AAGCTGCTTT CGGTCTCACC
    ACAGTGCACA GAAGGCTCCA TGGGGAGACA GGGACGGTCC ATGCAGCAAG TCAGCTCTGT
    AAGGAAGCAA TGGGGAAGCT GTACAATTTC AGCACTTCCT CCAGAAGTCA GGACAGAGAA
    GCTCTGTCTC AAGAAGTTAT GTCTGTGATT GCCCAGGTGA AGGAACATTT ACAAGTTCAA
    AGCTTCTCAA ATGTAGATGA CAGATCTTAT GTTCCCGAGA GTTTCGAGTG CAGGTTGGAT
    AAACTTATCT TGCATGGGCA AGGGGATTTC CAAAAAATCC TTGACACCTA TTCACAGCAC
    CATACTTCGG TGTGTGAAGT ATTTGAAAGT GATTGTGGAA ACAACAAAAA TGAACAGAAA
    GATGCAAAAA CAGGAGTCTG CATCACTGCT CTAAAAACAG AAATAAAAAA CATAGATACT
    GTGAGTACTA CTCAAGAAAA GCCACATTGT CAAAGAGACA CAGGAATATC TTCCTCCCTA
    ATGGGTAAGA ATGTTCAGAG GGAACTCAGA AGGGGAGGAA GGAGAAACTG GACCCATTCT
    GATGCATTTC GAGTCTCCTT GGATCAAGAT GTGGAGACTG AGACTGAGCC ATCGGACTAC
    AGCAATGGTG AGGGAGCTGT TTTCAACAAG TCTCTGAGTG GCAGCCAGAC TTCCAGTGCT
    TGGAGCAACT TATCAGGGTT TAGTTCCTCT GCAAGCTGGG AGGAAGTGAA TTATCACGTT
    GACGACAGGT CAGCCAGAAA AGAGCCTGGC AAAGAACATC TGGTGGACAC TCAGTGTTCC
    ACTGCCTTGT CTGAGGAGCT AGAGAATGAC AGGGAAGGCA GAGCTATGCA TTCATTGCAT
    TCACAGCTTC ATGATCTCTC TCTTCAGGAA CCCAACAATG ACAATTTGGA GCCTTCTCAA
    AATCAGCCAC AGCAACAGAT GCCCTTGACA CCCTTCTCGC CTCATAATAC CCCAGGCATT
    TTCTTGGCCC CTGGTGCAGG GCTTCTAGAA GGAGCTCCAG AAGGTATCCA GGAAGTCAGA
    AATATGGGAC CCAGAAATAC TTCTGCTCAC TCCAGACCCT CATATCGTTC TGCTTCTTGG
    TCTTCTGATT CTGGTAGGCC CAAGAATATG GGCACACATC CTTCAGTCCA AAAAGAAGAA
    GCCTTTGAAA TAATTGTTGA GTTTCCAGAA ACCAACTGCG ATGTCAAAGA CAGGCAGGGG
    AAAGAGCAGG GAGAAGAAAT TAGTGAAAGA GGCGCAGGCC CTACATTTAA AGCTAGTCCC
    TCCTGGGTTG ACCCAGAAGG AGAAACAGCA GAAAGCACTG AAGATGCACC CTTAGACTTT
    CACAGGGTCC TGCACAATTC TCTGGGAAAC ATTTCCATGC TGCCATGTAG CTCCTTCACC
    CCTAATTGGC CTGTTCAAAA TCCTGACTCC AGAAAAAGTG GTGGCCCAGT CGCAGAGCAG
    GGCATCGACC CTGATGCCTC CACAGTGGAT GAGGAGGGGC AACTGCTCGA CAGCATGGAT
    GTTCCCTGCA CAAATGGGCA CGGCTCTCAT AGACTGTGCA TTCTGAGACA GCCGCCTGGT
    CAGAGGGCGG AGACCCCCAA TTCCTCTGTA AGCGGTAACA TCCTCTTCCC TGTCCTCAGC
    GAGGACTGCA CTACCACAGA GGAAGGAAAT CAGCCTGGAA ACATGCTAAA CTGCAGCCAG
    AACTCCAGCT CATCCTCAGT GTGGTGGCTG AAATCACCTG CATTTTCCAG TGGTTCTTCT
    GAGGGGGACA GCCCTTGGTC CTATCTGAAT TCCAGTGGGA GTTCTTGGGT TTCATTGCCG
    GGAAAGATGA GGAAAGAGAT CCTTGAGGCT CGCACCTTGC AACCTGATGA CTTTGAAAAG
    CTGTTGGCAG GAGTGAGGCA TGATTGGCTG TTTCAGAGAC TAGAGAATAC GGGGGTTTTT
    AAGCCCAGTC AACTCCACCG AGCACATAGT GCTCTTTTGT TAAAATATTC AAAAAAATCT
    GAACTGTGGA CGGCCCAGGA AACTATTGTC TATTTGGGGG ACTACTTGAC TGTGAAGAAA
    AAAGGCAGAC AAAGAAATGC TTTTTGGGTT CATCATCTTC ATCAAGAAGA AATTCTGGGG
    AGGTATGTTG GGAAAGACTA TAAGGAGCAG AAGGGGCTCT GGCACCACTT CACTGATGTG
    GAGCGACAGA TGACCGCACA GCACTATGTG ACAGAATTTA ACAAGAGACT CTATGAACAA
    AACATTCCCA CCCAGATATT CTACATCCCA TCCACAATAC TACTGATTTT AGAGGACAAG
    ACAATAAAGG GATGTATCAG TGTGGAGCCT TACATACTGG GAGAATTTGT AAAATTGTCA
    AATAACACGA AAGTGGTGAA AACAGAATAC AAAGCCACAG AATATGGCTT GGCCTATGGC
    CATTTTTCTT ATGAGTTTTC TAATCATAGA GATGTTGTGG TCGATTTACA AGGTTGGGTA
    ACCGGTAATG GAAAAGGACT CATCTACCTC ACAGATCCCC AGATTCACTC CGTTGATCAG
    AAAGTTTTCA CTACCAATTT TGGAAAGAGA GGAATTTTTT ACTTCTTTAA TAACCAGCAT
    GTGGAATGTA ATGAAATCTG CCATCGTCTT TCTTTGACTA GACCTTCAAT GGAGAAACCA
    TGTAAGTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864138.1
    CDS79..3210
    Translation

    Target ORF information:

    RefSeq Version XM_017008649.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008649.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTGGTACG AAGCAGCAGA GTTAATATGG GCCTCCATTG TAGGATATTT GGCACTTCCT 
    CAGCCGGATA AAAAGGGCCT CTCCACGTCG CTAGGTATAC TGGCAGACAT CTTTGTTTCC
    ATGAGCAAGA ACGATTATGA AAAGTTTAAA AACAATCCAC AAATTAATTT GCCTCCTGTT
    TTCTCACAGA GCCTGCTGAA GGAGTTTGAC CACCATTTGC TGTCCGCTGC AGAAGCCTGC
    AAGCTGGCAG CTGCCTTCAG TGCCTATACG CCGCTCTTCG TGCTCACAGC TGTGAATATC
    CGTGGCACGT GTTTATTGTC CTACAGTAGT TCAAATGACT GTCCTCCAGA ATTGAAAAAC
    TTACATCTGT GTGAAGCCAA AGAGGCCTTT GAGATTGGCC TCCTCACCAA GAGAGATGAT
    GAGCCTGTTA CTGGAAAACA GGAGCTTCAC AGCTTTGTCA AAGCTGCTTT CGGTCTCACC
    ACAGTGCACA GAAGGCTCCA TGGGGAGACA GGGACGGTCC ATGCAGCAAG TCAGCTCTGT
    AAGGAAGCAA TGGGGAAGCT GTACAATTTC AGCACTTCCT CCAGAAGTCA GGACAGAGAA
    GCTCTGTCTC AAGAAGTTAT GTCTGTGATT GCCCAGGTGA AGGAACATTT ACAAGTTCAA
    AGCTTCTCAA ATGTAGATGA CAGATCTTAT GTTCCCGAGA GTTTCGAGTG CAGGTTGGAT
    AAACTTATCT TGCATGGGCA AGGGGATTTC CAAAAAATCC TTGACACCTA TTCACAGCAC
    CATACTTCGG TGTGTGAAGT ATTTGAAAGT GATTGTGGAA ACAACAAAAA TGAACAGAAA
    GATGCAAAAA CAGGAGTCTG CATCACTGCT CTAAAAACAG AAATAAAAAA CATAGATACT
    GTGAGTACTA CTCAAGAAAA GCCACATTGT CAAAGAGACA CAGGAATATC TTCCTCCCTA
    ATGGGTAAGA ATGTTCAGAG GGAACTCAGA AGGGGAGGAA GGAGAAACTG GACCCATTCT
    GATGCATTTC GAGTCTCCTT GGATCAAGAT GTGGAGACTG AGACTGAGCC ATCGGACTAC
    AGCAATGGTG AGGGAGCTGT TTTCAACAAG TCTCTGAGTG GCAGCCAGAC TTCCAGTGCT
    TGGAGCAACT TATCAGGGTT TAGTTCCTCT GCAAGCTGGG AGGAAGTGAA TTATCACGTT
    GACGACAGGT CAGCCAGAAA AGAGCCTGGC AAAGAACATC TGGTGGACAC TCAGTGTTCC
    ACTGCCTTGT CTGAGGAGCT AGAGAATGAC AGGGAAGGCA GAGCTATGCA TTCATTGCAT
    TCACAGCTTC ATGATCTCTC TCTTCAGGAA CCCAACAATG ACAATTTGGA GCCTTCTCAA
    AATCAGCCAC AGCAACAGAT GCCCTTGACA CCCTTCTCGC CTCATAATAC CCCAGGCATT
    TTCTTGGCCC CTGGTGCAGG GCTTCTAGAA GGAGCTCCAG AAGGTATCCA GGAAGTCAGA
    AATATGGGAC CCAGAAATAC TTCTGCTCAC TCCAGACCCT CATATCGTTC TGCTTCTTGG
    TCTTCTGATT CTGGTAGGCC CAAGAATATG GGCACACATC CTTCAGTCCA AAAAGAAGAA
    GCCTTTGAAA TAATTGTTGA GTTTCCAGAA ACCAACTGCG ATGTCAAAGA CAGGCAGGGG
    AAAGAGCAGG GAGAAGAAAT TAGTGAAAGA GGCGCAGGCC CTACATTTAA AGCTAGTCCC
    TCCTGGGTTG ACCCAGAAGG AGAAACAGCA GAAAGCACTG AAGATGCACC CTTAGACTTT
    CACAGGGTCC TGCACAATTC TCTGGGAAAC ATTTCCATGC TGCCATGTAG CTCCTTCACC
    CCTAATTGGC CTGTTCAAAA TCCTGACTCC AGAAAAAGTG GTGGCCCAGT CGCAGAGCAG
    GGCATCGACC CTGATGCCTC CACAGTGGAT GAGGAGGGGC AACTGCTCGA CAGCATGGAT
    GTTCCCTGCA CAAATGGGCA CGGCTCTCAT AGACTGTGCA TTCTGAGACA GCCGCCTGGT
    CAGAGGGCGG AGACCCCCAA TTCCTCTGTA AGCGGTAACA TCCTCTTCCC TGTCCTCAGC
    GAGGACTGCA CTACCACAGA GGAAGGAAAT CAGCCTGGAA ACATGCTAAA CTGCAGCCAG
    AACTCCAGCT CATCCTCAGT GTGGTGGCTG AAATCACCTG CATTTTCCAG TGGTTCTTCT
    GAGGGGGACA GCCCTTGGTC CTATCTGAAT TCCAGTGGGA GTTCTTGGGT TTCATTGCCG
    GGAAAGATGA GGAAAGAGAT CCTTGAGGCT CGCACCTTGC AACCTGATGA CTTTGAAAAG
    CTGTTGGCAG GAGTGAGGCA TGATTGGCTG TTTCAGAGAC TAGAGAATAC GGGGGTTTTT
    AAGCCCAGTC AACTCCACCG AGCACATAGT GCTCTTTTGT TAAAATATTC AAAAAAATCT
    GAACTGTGGA CGGCCCAGGA AACTATTGTC TATTTGGGGG ACTACTTGAC TGTGAAGAAA
    AAAGGCAGAC AAAGAAATGC TTTTTGGGTT CATCATCTTC ATCAAGAAGA AATTCTGGGG
    AGGTATGTTG GGAAAGACTA TAAGGAGCAG AAGGGGCTCT GGCACCACTT CACTGATGTG
    GAGCGACAGA TGACCGCACA GCACTATGTG ACAGAATTTA ACAAGAGACT CTATGAACAA
    AACATTCCCA CCCAGATATT CTACATCCCA TCCACAATAC TACTGATTTT AGAGGACAAG
    ACAATAAAGG GATGTATCAG TGTGGAGCCT TACATACTGG GAGAATTTGT AAAATTGTCA
    AATAACACGA AAGTGGTGAA AACAGAATAC AAAGCCACAG AATATGGCTT GGCCTATGGC
    CATTTTTCTT ATGAGTTTTC TAATCATAGA GATGTTGTGG TCGATTTACA AGGTTGGGTA
    ACCGGTAATG GAAAAGGACT CATCTACCTC ACAGATCCCC AGATTCACTC CGTTGATCAG
    AAAGTTTTCA CTACCAATTT TGGAAAGAGA GGAATTTTTT ACTTCTTTAA TAACCAGCAT
    GTGGAATGTA ATGAAATCTG CCATCGTCTT TCTTTGACTA GACCTTCAAT GGAGAAACCA
    TGTAAGTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95418
    Clone ID Related Accession (Same CDS sequence) XM_017008649.1 , XM_017008649.2
    Accession Version XM_017008649.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3132bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017008649.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTGGTACG AAGCAGCAGA GTTAATATGG GCCTCCATTG TAGGATATTT GGCACTTCCT 
    CAGCCGGATA AAAAGGGCCT CTCCACGTCG CTAGGTATAC TGGCAGACAT CTTTGTTTCC
    ATGAGCAAGA ACGATTATGA AAAGTTTAAA AACAATCCAC AAATTAATTT GCCTCCTGTT
    TTCTCACAGA GCCTGCTGAA GGAGTTTGAC CACCATTTGC TGTCCGCTGC AGAAGCCTGC
    AAGCTGGCAG CTGCCTTCAG TGCCTATACG CCGCTCTTCG TGCTCACAGC TGTGAATATC
    CGTGGCACGT GTTTATTGTC CTACAGTAGT TCAAATGACT GTCCTCCAGA ATTGAAAAAC
    TTACATCTGT GTGAAGCCAA AGAGGCCTTT GAGATTGGCC TCCTCACCAA GAGAGATGAT
    GAGCCTGTTA CTGGAAAACA GGAGCTTCAC AGCTTTGTCA AAGCTGCTTT CGGTCTCACC
    ACAGTGCACA GAAGGCTCCA TGGGGAGACA GGGACGGTCC ATGCAGCAAG TCAGCTCTGT
    AAGGAAGCAA TGGGGAAGCT GTACAATTTC AGCACTTCCT CCAGAAGTCA GGACAGAGAA
    GCTCTGTCTC AAGAAGTTAT GTCTGTGATT GCCCAGGTGA AGGAACATTT ACAAGTTCAA
    AGCTTCTCAA ATGTAGATGA CAGATCTTAT GTTCCCGAGA GTTTCGAGTG CAGGTTGGAT
    AAACTTATCT TGCATGGGCA AGGGGATTTC CAAAAAATCC TTGACACCTA TTCACAGCAC
    CATACTTCGG TGTGTGAAGT ATTTGAAAGT GATTGTGGAA ACAACAAAAA TGAACAGAAA
    GATGCAAAAA CAGGAGTCTG CATCACTGCT CTAAAAACAG AAATAAAAAA CATAGATACT
    GTGAGTACTA CTCAAGAAAA GCCACATTGT CAAAGAGACA CAGGAATATC TTCCTCCCTA
    ATGGGTAAGA ATGTTCAGAG GGAACTCAGA AGGGGAGGAA GGAGAAACTG GACCCATTCT
    GATGCATTTC GAGTCTCCTT GGATCAAGAT GTGGAGACTG AGACTGAGCC ATCGGACTAC
    AGCAATGGTG AGGGAGCTGT TTTCAACAAG TCTCTGAGTG GCAGCCAGAC TTCCAGTGCT
    TGGAGCAACT TATCAGGGTT TAGTTCCTCT GCAAGCTGGG AGGAAGTGAA TTATCACGTT
    GACGACAGGT CAGCCAGAAA AGAGCCTGGC AAAGAACATC TGGTGGACAC TCAGTGTTCC
    ACTGCCTTGT CTGAGGAGCT AGAGAATGAC AGGGAAGGCA GAGCTATGCA TTCATTGCAT
    TCACAGCTTC ATGATCTCTC TCTTCAGGAA CCCAACAATG ACAATTTGGA GCCTTCTCAA
    AATCAGCCAC AGCAACAGAT GCCCTTGACA CCCTTCTCGC CTCATAATAC CCCAGGCATT
    TTCTTGGCCC CTGGTGCAGG GCTTCTAGAA GGAGCTCCAG AAGGTATCCA GGAAGTCAGA
    AATATGGGAC CCAGAAATAC TTCTGCTCAC TCCAGACCCT CATATCGTTC TGCTTCTTGG
    TCTTCTGATT CTGGTAGGCC CAAGAATATG GGCACACATC CTTCAGTCCA AAAAGAAGAA
    GCCTTTGAAA TAATTGTTGA GTTTCCAGAA ACCAACTGCG ATGTCAAAGA CAGGCAGGGG
    AAAGAGCAGG GAGAAGAAAT TAGTGAAAGA GGCGCAGGCC CTACATTTAA AGCTAGTCCC
    TCCTGGGTTG ACCCAGAAGG AGAAACAGCA GAAAGCACTG AAGATGCACC CTTAGACTTT
    CACAGGGTCC TGCACAATTC TCTGGGAAAC ATTTCCATGC TGCCATGTAG CTCCTTCACC
    CCTAATTGGC CTGTTCAAAA TCCTGACTCC AGAAAAAGTG GTGGCCCAGT CGCAGAGCAG
    GGCATCGACC CTGATGCCTC CACAGTGGAT GAGGAGGGGC AACTGCTCGA CAGCATGGAT
    GTTCCCTGCA CAAATGGGCA CGGCTCTCAT AGACTGTGCA TTCTGAGACA GCCGCCTGGT
    CAGAGGGCGG AGACCCCCAA TTCCTCTGTA AGCGGTAACA TCCTCTTCCC TGTCCTCAGC
    GAGGACTGCA CTACCACAGA GGAAGGAAAT CAGCCTGGAA ACATGCTAAA CTGCAGCCAG
    AACTCCAGCT CATCCTCAGT GTGGTGGCTG AAATCACCTG CATTTTCCAG TGGTTCTTCT
    GAGGGGGACA GCCCTTGGTC CTATCTGAAT TCCAGTGGGA GTTCTTGGGT TTCATTGCCG
    GGAAAGATGA GGAAAGAGAT CCTTGAGGCT CGCACCTTGC AACCTGATGA CTTTGAAAAG
    CTGTTGGCAG GAGTGAGGCA TGATTGGCTG TTTCAGAGAC TAGAGAATAC GGGGGTTTTT
    AAGCCCAGTC AACTCCACCG AGCACATAGT GCTCTTTTGT TAAAATATTC AAAAAAATCT
    GAACTGTGGA CGGCCCAGGA AACTATTGTC TATTTGGGGG ACTACTTGAC TGTGAAGAAA
    AAAGGCAGAC AAAGAAATGC TTTTTGGGTT CATCATCTTC ATCAAGAAGA AATTCTGGGG
    AGGTATGTTG GGAAAGACTA TAAGGAGCAG AAGGGGCTCT GGCACCACTT CACTGATGTG
    GAGCGACAGA TGACCGCACA GCACTATGTG ACAGAATTTA ACAAGAGACT CTATGAACAA
    AACATTCCCA CCCAGATATT CTACATCCCA TCCACAATAC TACTGATTTT AGAGGACAAG
    ACAATAAAGG GATGTATCAG TGTGGAGCCT TACATACTGG GAGAATTTGT AAAATTGTCA
    AATAACACGA AAGTGGTGAA AACAGAATAC AAAGCCACAG AATATGGCTT GGCCTATGGC
    CATTTTTCTT ATGAGTTTTC TAATCATAGA GATGTTGTGG TCGATTTACA AGGTTGGGTA
    ACCGGTAATG GAAAAGGACT CATCTACCTC ACAGATCCCC AGATTCACTC CGTTGATCAG
    AAAGTTTTCA CTACCAATTT TGGAAAGAGA GGAATTTTTT ACTTCTTTAA TAACCAGCAT
    GTGGAATGTA ATGAAATCTG CCATCGTCTT TCTTTGACTA GACCTTCAAT GGAGAAACCA
    TGTAAGTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864138.1
    CDS80..3211
    Translation

    Target ORF information:

    RefSeq Version XM_017008649.2
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008649.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTGGTACG AAGCAGCAGA GTTAATATGG GCCTCCATTG TAGGATATTT GGCACTTCCT 
    CAGCCGGATA AAAAGGGCCT CTCCACGTCG CTAGGTATAC TGGCAGACAT CTTTGTTTCC
    ATGAGCAAGA ACGATTATGA AAAGTTTAAA AACAATCCAC AAATTAATTT GCCTCCTGTT
    TTCTCACAGA GCCTGCTGAA GGAGTTTGAC CACCATTTGC TGTCCGCTGC AGAAGCCTGC
    AAGCTGGCAG CTGCCTTCAG TGCCTATACG CCGCTCTTCG TGCTCACAGC TGTGAATATC
    CGTGGCACGT GTTTATTGTC CTACAGTAGT TCAAATGACT GTCCTCCAGA ATTGAAAAAC
    TTACATCTGT GTGAAGCCAA AGAGGCCTTT GAGATTGGCC TCCTCACCAA GAGAGATGAT
    GAGCCTGTTA CTGGAAAACA GGAGCTTCAC AGCTTTGTCA AAGCTGCTTT CGGTCTCACC
    ACAGTGCACA GAAGGCTCCA TGGGGAGACA GGGACGGTCC ATGCAGCAAG TCAGCTCTGT
    AAGGAAGCAA TGGGGAAGCT GTACAATTTC AGCACTTCCT CCAGAAGTCA GGACAGAGAA
    GCTCTGTCTC AAGAAGTTAT GTCTGTGATT GCCCAGGTGA AGGAACATTT ACAAGTTCAA
    AGCTTCTCAA ATGTAGATGA CAGATCTTAT GTTCCCGAGA GTTTCGAGTG CAGGTTGGAT
    AAACTTATCT TGCATGGGCA AGGGGATTTC CAAAAAATCC TTGACACCTA TTCACAGCAC
    CATACTTCGG TGTGTGAAGT ATTTGAAAGT GATTGTGGAA ACAACAAAAA TGAACAGAAA
    GATGCAAAAA CAGGAGTCTG CATCACTGCT CTAAAAACAG AAATAAAAAA CATAGATACT
    GTGAGTACTA CTCAAGAAAA GCCACATTGT CAAAGAGACA CAGGAATATC TTCCTCCCTA
    ATGGGTAAGA ATGTTCAGAG GGAACTCAGA AGGGGAGGAA GGAGAAACTG GACCCATTCT
    GATGCATTTC GAGTCTCCTT GGATCAAGAT GTGGAGACTG AGACTGAGCC ATCGGACTAC
    AGCAATGGTG AGGGAGCTGT TTTCAACAAG TCTCTGAGTG GCAGCCAGAC TTCCAGTGCT
    TGGAGCAACT TATCAGGGTT TAGTTCCTCT GCAAGCTGGG AGGAAGTGAA TTATCACGTT
    GACGACAGGT CAGCCAGAAA AGAGCCTGGC AAAGAACATC TGGTGGACAC TCAGTGTTCC
    ACTGCCTTGT CTGAGGAGCT AGAGAATGAC AGGGAAGGCA GAGCTATGCA TTCATTGCAT
    TCACAGCTTC ATGATCTCTC TCTTCAGGAA CCCAACAATG ACAATTTGGA GCCTTCTCAA
    AATCAGCCAC AGCAACAGAT GCCCTTGACA CCCTTCTCGC CTCATAATAC CCCAGGCATT
    TTCTTGGCCC CTGGTGCAGG GCTTCTAGAA GGAGCTCCAG AAGGTATCCA GGAAGTCAGA
    AATATGGGAC CCAGAAATAC TTCTGCTCAC TCCAGACCCT CATATCGTTC TGCTTCTTGG
    TCTTCTGATT CTGGTAGGCC CAAGAATATG GGCACACATC CTTCAGTCCA AAAAGAAGAA
    GCCTTTGAAA TAATTGTTGA GTTTCCAGAA ACCAACTGCG ATGTCAAAGA CAGGCAGGGG
    AAAGAGCAGG GAGAAGAAAT TAGTGAAAGA GGCGCAGGCC CTACATTTAA AGCTAGTCCC
    TCCTGGGTTG ACCCAGAAGG AGAAACAGCA GAAAGCACTG AAGATGCACC CTTAGACTTT
    CACAGGGTCC TGCACAATTC TCTGGGAAAC ATTTCCATGC TGCCATGTAG CTCCTTCACC
    CCTAATTGGC CTGTTCAAAA TCCTGACTCC AGAAAAAGTG GTGGCCCAGT CGCAGAGCAG
    GGCATCGACC CTGATGCCTC CACAGTGGAT GAGGAGGGGC AACTGCTCGA CAGCATGGAT
    GTTCCCTGCA CAAATGGGCA CGGCTCTCAT AGACTGTGCA TTCTGAGACA GCCGCCTGGT
    CAGAGGGCGG AGACCCCCAA TTCCTCTGTA AGCGGTAACA TCCTCTTCCC TGTCCTCAGC
    GAGGACTGCA CTACCACAGA GGAAGGAAAT CAGCCTGGAA ACATGCTAAA CTGCAGCCAG
    AACTCCAGCT CATCCTCAGT GTGGTGGCTG AAATCACCTG CATTTTCCAG TGGTTCTTCT
    GAGGGGGACA GCCCTTGGTC CTATCTGAAT TCCAGTGGGA GTTCTTGGGT TTCATTGCCG
    GGAAAGATGA GGAAAGAGAT CCTTGAGGCT CGCACCTTGC AACCTGATGA CTTTGAAAAG
    CTGTTGGCAG GAGTGAGGCA TGATTGGCTG TTTCAGAGAC TAGAGAATAC GGGGGTTTTT
    AAGCCCAGTC AACTCCACCG AGCACATAGT GCTCTTTTGT TAAAATATTC AAAAAAATCT
    GAACTGTGGA CGGCCCAGGA AACTATTGTC TATTTGGGGG ACTACTTGAC TGTGAAGAAA
    AAAGGCAGAC AAAGAAATGC TTTTTGGGTT CATCATCTTC ATCAAGAAGA AATTCTGGGG
    AGGTATGTTG GGAAAGACTA TAAGGAGCAG AAGGGGCTCT GGCACCACTT CACTGATGTG
    GAGCGACAGA TGACCGCACA GCACTATGTG ACAGAATTTA ACAAGAGACT CTATGAACAA
    AACATTCCCA CCCAGATATT CTACATCCCA TCCACAATAC TACTGATTTT AGAGGACAAG
    ACAATAAAGG GATGTATCAG TGTGGAGCCT TACATACTGG GAGAATTTGT AAAATTGTCA
    AATAACACGA AAGTGGTGAA AACAGAATAC AAAGCCACAG AATATGGCTT GGCCTATGGC
    CATTTTTCTT ATGAGTTTTC TAATCATAGA GATGTTGTGG TCGATTTACA AGGTTGGGTA
    ACCGGTAATG GAAAAGGACT CATCTACCTC ACAGATCCCC AGATTCACTC CGTTGATCAG
    AAAGTTTTCA CTACCAATTT TGGAAAGAGA GGAATTTTTT ACTTCTTTAA TAACCAGCAT
    GTGGAATGTA ATGAAATCTG CCATCGTCTT TCTTTGACTA GACCTTCAAT GGAGAAACCA
    TGTAAGTCAT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95419
    Clone ID Related Accession (Same CDS sequence) XM_017008650.1 , XM_017008651.1
    Accession Version XM_017008650.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3114bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTGGTACG AAGCAGCAGA GTTAATATGG GCCTCCATTG TAGGATATTT GGCACTTCCT 
    CAGCCGGATA AAAAGGGCCT CTCCACGTCG CTAGGTATAC TGGCAGACAT CTTTGTTTCC
    ATGAGCAAGA ACGATTATGA AAAGTTTAAA AACAATCCAC AAATTAATTT GAGCCTGCTG
    AAGGAGTTTG ACCACCATTT GCTGTCCGCT GCAGAAGCCT GCAAGCTGGC AGCTGCCTTC
    AGTGCCTATA CGCCGCTCTT CGTGCTCACA GCTGTGAATA TCCGTGGCAC GTGTTTATTG
    TCCTACAGTA GTTCAAATGA CTGTCCTCCA GAATTGAAAA ACTTACATCT GTGTGAAGCC
    AAAGAGGCCT TTGAGATTGG CCTCCTCACC AAGAGAGATG ATGAGCCTGT TACTGGAAAA
    CAGGAGCTTC ACAGCTTTGT CAAAGCTGCT TTCGGTCTCA CCACAGTGCA CAGAAGGCTC
    CATGGGGAGA CAGGGACGGT CCATGCAGCA AGTCAGCTCT GTAAGGAAGC AATGGGGAAG
    CTGTACAATT TCAGCACTTC CTCCAGAAGT CAGGACAGAG AAGCTCTGTC TCAAGAAGTT
    ATGTCTGTGA TTGCCCAGGT GAAGGAACAT TTACAAGTTC AAAGCTTCTC AAATGTAGAT
    GACAGATCTT ATGTTCCCGA GAGTTTCGAG TGCAGGTTGG ATAAACTTAT CTTGCATGGG
    CAAGGGGATT TCCAAAAAAT CCTTGACACC TATTCACAGC ACCATACTTC GGTGTGTGAA
    GTATTTGAAA GTGATTGTGG AAACAACAAA AATGAACAGA AAGATGCAAA AACAGGAGTC
    TGCATCACTG CTCTAAAAAC AGAAATAAAA AACATAGATA CTGTGAGTAC TACTCAAGAA
    AAGCCACATT GTCAAAGAGA CACAGGAATA TCTTCCTCCC TAATGGGTAA GAATGTTCAG
    AGGGAACTCA GAAGGGGAGG AAGGAGAAAC TGGACCCATT CTGATGCATT TCGAGTCTCC
    TTGGATCAAG ATGTGGAGAC TGAGACTGAG CCATCGGACT ACAGCAATGG TGAGGGAGCT
    GTTTTCAACA AGTCTCTGAG TGGCAGCCAG ACTTCCAGTG CTTGGAGCAA CTTATCAGGG
    TTTAGTTCCT CTGCAAGCTG GGAGGAAGTG AATTATCACG TTGACGACAG GTCAGCCAGA
    AAAGAGCCTG GCAAAGAACA TCTGGTGGAC ACTCAGTGTT CCACTGCCTT GTCTGAGGAG
    CTAGAGAATG ACAGGGAAGG CAGAGCTATG CATTCATTGC ATTCACAGCT TCATGATCTC
    TCTCTTCAGG AACCCAACAA TGACAATTTG GAGCCTTCTC AAAATCAGCC ACAGCAACAG
    ATGCCCTTGA CACCCTTCTC GCCTCATAAT ACCCCAGGCA TTTTCTTGGC CCCTGGTGCA
    GGGCTTCTAG AAGGAGCTCC AGAAGGTATC CAGGAAGTCA GAAATATGGG ACCCAGAAAT
    ACTTCTGCTC ACTCCAGACC CTCATATCGT TCTGCTTCTT GGTCTTCTGA TTCTGGTAGG
    CCCAAGAATA TGGGCACACA TCCTTCAGTC CAAAAAGAAG AAGCCTTTGA AATAATTGTT
    GAGTTTCCAG AAACCAACTG CGATGTCAAA GACAGGCAGG GGAAAGAGCA GGGAGAAGAA
    ATTAGTGAAA GAGGCGCAGG CCCTACATTT AAAGCTAGTC CCTCCTGGGT TGACCCAGAA
    GGAGAAACAG CAGAAAGCAC TGAAGATGCA CCCTTAGACT TTCACAGGGT CCTGCACAAT
    TCTCTGGGAA ACATTTCCAT GCTGCCATGT AGCTCCTTCA CCCCTAATTG GCCTGTTCAA
    AATCCTGACT CCAGAAAAAG TGGTGGCCCA GTCGCAGAGC AGGGCATCGA CCCTGATGCC
    TCCACAGTGG ATGAGGAGGG GCAACTGCTC GACAGCATGG ATGTTCCCTG CACAAATGGG
    CACGGCTCTC ATAGACTGTG CATTCTGAGA CAGCCGCCTG GTCAGAGGGC GGAGACCCCC
    AATTCCTCTG TAAGCGGTAA CATCCTCTTC CCTGTCCTCA GCGAGGACTG CACTACCACA
    GAGGAAGGAA ATCAGCCTGG AAACATGCTA AACTGCAGCC AGAACTCCAG CTCATCCTCA
    GTGTGGTGGC TGAAATCACC TGCATTTTCC AGTGGTTCTT CTGAGGGGGA CAGCCCTTGG
    TCCTATCTGA ATTCCAGTGG GAGTTCTTGG GTTTCATTGC CGGGAAAGAT GAGGAAAGAG
    ATCCTTGAGG CTCGCACCTT GCAACCTGAT GACTTTGAAA AGCTGTTGGC AGGAGTGAGG
    CATGATTGGC TGTTTCAGAG ACTAGAGAAT ACGGGGGTTT TTAAGCCCAG TCAACTCCAC
    CGAGCACATA GTGCTCTTTT GTTAAAATAT TCAAAAAAAT CTGAACTGTG GACGGCCCAG
    GAAACTATTG TCTATTTGGG GGACTACTTG ACTGTGAAGA AAAAAGGCAG ACAAAGAAAT
    GCTTTTTGGG TTCATCATCT TCATCAAGAA GAAATTCTGG GGAGGTATGT TGGGAAAGAC
    TATAAGGAGC AGAAGGGGCT CTGGCACCAC TTCACTGATG TGGAGCGACA GATGACCGCA
    CAGCACTATG TGACAGAATT TAACAAGAGA CTCTATGAAC AAAACATTCC CACCCAGATA
    TTCTACATCC CATCCACAAT ACTACTGATT TTAGAGGACA AGACAATAAA GGGATGTATC
    AGTGTGGAGC CTTACATACT GGGAGAATTT GTAAAATTGT CAAATAACAC GAAAGTGGTG
    AAAACAGAAT ACAAAGCCAC AGAATATGGC TTGGCCTATG GCCATTTTTC TTATGAGTTT
    TCTAATCATA GAGATGTTGT GGTCGATTTA CAAGGTTGGG TAACCGGTAA TGGAAAAGGA
    CTCATCTACC TCACAGATCC CCAGATTCAC TCCGTTGATC AGAAAGTTTT CACTACCAAT
    TTTGGAAAGA GAGGAATTTT TTACTTCTTT AATAACCAGC ATGTGGAATG TAATGAAATC
    TGCCATCGTC TTTCTTTGAC TAGACCTTCA ATGGAGAAAC CATGTAAGTC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864139.1
    CDS2507..5620
    Translation

    Target ORF information:

    RefSeq Version XM_017008650.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008650.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTGGTACG AAGCAGCAGA GTTAATATGG GCCTCCATTG TAGGATATTT GGCACTTCCT 
    CAGCCGGATA AAAAGGGCCT CTCCACGTCG CTAGGTATAC TGGCAGACAT CTTTGTTTCC
    ATGAGCAAGA ACGATTATGA AAAGTTTAAA AACAATCCAC AAATTAATTT GAGCCTGCTG
    AAGGAGTTTG ACCACCATTT GCTGTCCGCT GCAGAAGCCT GCAAGCTGGC AGCTGCCTTC
    AGTGCCTATA CGCCGCTCTT CGTGCTCACA GCTGTGAATA TCCGTGGCAC GTGTTTATTG
    TCCTACAGTA GTTCAAATGA CTGTCCTCCA GAATTGAAAA ACTTACATCT GTGTGAAGCC
    AAAGAGGCCT TTGAGATTGG CCTCCTCACC AAGAGAGATG ATGAGCCTGT TACTGGAAAA
    CAGGAGCTTC ACAGCTTTGT CAAAGCTGCT TTCGGTCTCA CCACAGTGCA CAGAAGGCTC
    CATGGGGAGA CAGGGACGGT CCATGCAGCA AGTCAGCTCT GTAAGGAAGC AATGGGGAAG
    CTGTACAATT TCAGCACTTC CTCCAGAAGT CAGGACAGAG AAGCTCTGTC TCAAGAAGTT
    ATGTCTGTGA TTGCCCAGGT GAAGGAACAT TTACAAGTTC AAAGCTTCTC AAATGTAGAT
    GACAGATCTT ATGTTCCCGA GAGTTTCGAG TGCAGGTTGG ATAAACTTAT CTTGCATGGG
    CAAGGGGATT TCCAAAAAAT CCTTGACACC TATTCACAGC ACCATACTTC GGTGTGTGAA
    GTATTTGAAA GTGATTGTGG AAACAACAAA AATGAACAGA AAGATGCAAA AACAGGAGTC
    TGCATCACTG CTCTAAAAAC AGAAATAAAA AACATAGATA CTGTGAGTAC TACTCAAGAA
    AAGCCACATT GTCAAAGAGA CACAGGAATA TCTTCCTCCC TAATGGGTAA GAATGTTCAG
    AGGGAACTCA GAAGGGGAGG AAGGAGAAAC TGGACCCATT CTGATGCATT TCGAGTCTCC
    TTGGATCAAG ATGTGGAGAC TGAGACTGAG CCATCGGACT ACAGCAATGG TGAGGGAGCT
    GTTTTCAACA AGTCTCTGAG TGGCAGCCAG ACTTCCAGTG CTTGGAGCAA CTTATCAGGG
    TTTAGTTCCT CTGCAAGCTG GGAGGAAGTG AATTATCACG TTGACGACAG GTCAGCCAGA
    AAAGAGCCTG GCAAAGAACA TCTGGTGGAC ACTCAGTGTT CCACTGCCTT GTCTGAGGAG
    CTAGAGAATG ACAGGGAAGG CAGAGCTATG CATTCATTGC ATTCACAGCT TCATGATCTC
    TCTCTTCAGG AACCCAACAA TGACAATTTG GAGCCTTCTC AAAATCAGCC ACAGCAACAG
    ATGCCCTTGA CACCCTTCTC GCCTCATAAT ACCCCAGGCA TTTTCTTGGC CCCTGGTGCA
    GGGCTTCTAG AAGGAGCTCC AGAAGGTATC CAGGAAGTCA GAAATATGGG ACCCAGAAAT
    ACTTCTGCTC ACTCCAGACC CTCATATCGT TCTGCTTCTT GGTCTTCTGA TTCTGGTAGG
    CCCAAGAATA TGGGCACACA TCCTTCAGTC CAAAAAGAAG AAGCCTTTGA AATAATTGTT
    GAGTTTCCAG AAACCAACTG CGATGTCAAA GACAGGCAGG GGAAAGAGCA GGGAGAAGAA
    ATTAGTGAAA GAGGCGCAGG CCCTACATTT AAAGCTAGTC CCTCCTGGGT TGACCCAGAA
    GGAGAAACAG CAGAAAGCAC TGAAGATGCA CCCTTAGACT TTCACAGGGT CCTGCACAAT
    TCTCTGGGAA ACATTTCCAT GCTGCCATGT AGCTCCTTCA CCCCTAATTG GCCTGTTCAA
    AATCCTGACT CCAGAAAAAG TGGTGGCCCA GTCGCAGAGC AGGGCATCGA CCCTGATGCC
    TCCACAGTGG ATGAGGAGGG GCAACTGCTC GACAGCATGG ATGTTCCCTG CACAAATGGG
    CACGGCTCTC ATAGACTGTG CATTCTGAGA CAGCCGCCTG GTCAGAGGGC GGAGACCCCC
    AATTCCTCTG TAAGCGGTAA CATCCTCTTC CCTGTCCTCA GCGAGGACTG CACTACCACA
    GAGGAAGGAA ATCAGCCTGG AAACATGCTA AACTGCAGCC AGAACTCCAG CTCATCCTCA
    GTGTGGTGGC TGAAATCACC TGCATTTTCC AGTGGTTCTT CTGAGGGGGA CAGCCCTTGG
    TCCTATCTGA ATTCCAGTGG GAGTTCTTGG GTTTCATTGC CGGGAAAGAT GAGGAAAGAG
    ATCCTTGAGG CTCGCACCTT GCAACCTGAT GACTTTGAAA AGCTGTTGGC AGGAGTGAGG
    CATGATTGGC TGTTTCAGAG ACTAGAGAAT ACGGGGGTTT TTAAGCCCAG TCAACTCCAC
    CGAGCACATA GTGCTCTTTT GTTAAAATAT TCAAAAAAAT CTGAACTGTG GACGGCCCAG
    GAAACTATTG TCTATTTGGG GGACTACTTG ACTGTGAAGA AAAAAGGCAG ACAAAGAAAT
    GCTTTTTGGG TTCATCATCT TCATCAAGAA GAAATTCTGG GGAGGTATGT TGGGAAAGAC
    TATAAGGAGC AGAAGGGGCT CTGGCACCAC TTCACTGATG TGGAGCGACA GATGACCGCA
    CAGCACTATG TGACAGAATT TAACAAGAGA CTCTATGAAC AAAACATTCC CACCCAGATA
    TTCTACATCC CATCCACAAT ACTACTGATT TTAGAGGACA AGACAATAAA GGGATGTATC
    AGTGTGGAGC CTTACATACT GGGAGAATTT GTAAAATTGT CAAATAACAC GAAAGTGGTG
    AAAACAGAAT ACAAAGCCAC AGAATATGGC TTGGCCTATG GCCATTTTTC TTATGAGTTT
    TCTAATCATA GAGATGTTGT GGTCGATTTA CAAGGTTGGG TAACCGGTAA TGGAAAAGGA
    CTCATCTACC TCACAGATCC CCAGATTCAC TCCGTTGATC AGAAAGTTTT CACTACCAAT
    TTTGGAAAGA GAGGAATTTT TTACTTCTTT AATAACCAGC ATGTGGAATG TAATGAAATC
    TGCCATCGTC TTTCTTTGAC TAGACCTTCA ATGGAGAAAC CATGTAAGTC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu95419
    Clone ID Related Accession (Same CDS sequence) XM_017008650.1 , XM_017008651.1
    Accession Version XM_017008651.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3114bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTGGTACG AAGCAGCAGA GTTAATATGG GCCTCCATTG TAGGATATTT GGCACTTCCT 
    CAGCCGGATA AAAAGGGCCT CTCCACGTCG CTAGGTATAC TGGCAGACAT CTTTGTTTCC
    ATGAGCAAGA ACGATTATGA AAAGTTTAAA AACAATCCAC AAATTAATTT GAGCCTGCTG
    AAGGAGTTTG ACCACCATTT GCTGTCCGCT GCAGAAGCCT GCAAGCTGGC AGCTGCCTTC
    AGTGCCTATA CGCCGCTCTT CGTGCTCACA GCTGTGAATA TCCGTGGCAC GTGTTTATTG
    TCCTACAGTA GTTCAAATGA CTGTCCTCCA GAATTGAAAA ACTTACATCT GTGTGAAGCC
    AAAGAGGCCT TTGAGATTGG CCTCCTCACC AAGAGAGATG ATGAGCCTGT TACTGGAAAA
    CAGGAGCTTC ACAGCTTTGT CAAAGCTGCT TTCGGTCTCA CCACAGTGCA CAGAAGGCTC
    CATGGGGAGA CAGGGACGGT CCATGCAGCA AGTCAGCTCT GTAAGGAAGC AATGGGGAAG
    CTGTACAATT TCAGCACTTC CTCCAGAAGT CAGGACAGAG AAGCTCTGTC TCAAGAAGTT
    ATGTCTGTGA TTGCCCAGGT GAAGGAACAT TTACAAGTTC AAAGCTTCTC AAATGTAGAT
    GACAGATCTT ATGTTCCCGA GAGTTTCGAG TGCAGGTTGG ATAAACTTAT CTTGCATGGG
    CAAGGGGATT TCCAAAAAAT CCTTGACACC TATTCACAGC ACCATACTTC GGTGTGTGAA
    GTATTTGAAA GTGATTGTGG AAACAACAAA AATGAACAGA AAGATGCAAA AACAGGAGTC
    TGCATCACTG CTCTAAAAAC AGAAATAAAA AACATAGATA CTGTGAGTAC TACTCAAGAA
    AAGCCACATT GTCAAAGAGA CACAGGAATA TCTTCCTCCC TAATGGGTAA GAATGTTCAG
    AGGGAACTCA GAAGGGGAGG AAGGAGAAAC TGGACCCATT CTGATGCATT TCGAGTCTCC
    TTGGATCAAG ATGTGGAGAC TGAGACTGAG CCATCGGACT ACAGCAATGG TGAGGGAGCT
    GTTTTCAACA AGTCTCTGAG TGGCAGCCAG ACTTCCAGTG CTTGGAGCAA CTTATCAGGG
    TTTAGTTCCT CTGCAAGCTG GGAGGAAGTG AATTATCACG TTGACGACAG GTCAGCCAGA
    AAAGAGCCTG GCAAAGAACA TCTGGTGGAC ACTCAGTGTT CCACTGCCTT GTCTGAGGAG
    CTAGAGAATG ACAGGGAAGG CAGAGCTATG CATTCATTGC ATTCACAGCT TCATGATCTC
    TCTCTTCAGG AACCCAACAA TGACAATTTG GAGCCTTCTC AAAATCAGCC ACAGCAACAG
    ATGCCCTTGA CACCCTTCTC GCCTCATAAT ACCCCAGGCA TTTTCTTGGC CCCTGGTGCA
    GGGCTTCTAG AAGGAGCTCC AGAAGGTATC CAGGAAGTCA GAAATATGGG ACCCAGAAAT
    ACTTCTGCTC ACTCCAGACC CTCATATCGT TCTGCTTCTT GGTCTTCTGA TTCTGGTAGG
    CCCAAGAATA TGGGCACACA TCCTTCAGTC CAAAAAGAAG AAGCCTTTGA AATAATTGTT
    GAGTTTCCAG AAACCAACTG CGATGTCAAA GACAGGCAGG GGAAAGAGCA GGGAGAAGAA
    ATTAGTGAAA GAGGCGCAGG CCCTACATTT AAAGCTAGTC CCTCCTGGGT TGACCCAGAA
    GGAGAAACAG CAGAAAGCAC TGAAGATGCA CCCTTAGACT TTCACAGGGT CCTGCACAAT
    TCTCTGGGAA ACATTTCCAT GCTGCCATGT AGCTCCTTCA CCCCTAATTG GCCTGTTCAA
    AATCCTGACT CCAGAAAAAG TGGTGGCCCA GTCGCAGAGC AGGGCATCGA CCCTGATGCC
    TCCACAGTGG ATGAGGAGGG GCAACTGCTC GACAGCATGG ATGTTCCCTG CACAAATGGG
    CACGGCTCTC ATAGACTGTG CATTCTGAGA CAGCCGCCTG GTCAGAGGGC GGAGACCCCC
    AATTCCTCTG TAAGCGGTAA CATCCTCTTC CCTGTCCTCA GCGAGGACTG CACTACCACA
    GAGGAAGGAA ATCAGCCTGG AAACATGCTA AACTGCAGCC AGAACTCCAG CTCATCCTCA
    GTGTGGTGGC TGAAATCACC TGCATTTTCC AGTGGTTCTT CTGAGGGGGA CAGCCCTTGG
    TCCTATCTGA ATTCCAGTGG GAGTTCTTGG GTTTCATTGC CGGGAAAGAT GAGGAAAGAG
    ATCCTTGAGG CTCGCACCTT GCAACCTGAT GACTTTGAAA AGCTGTTGGC AGGAGTGAGG
    CATGATTGGC TGTTTCAGAG ACTAGAGAAT ACGGGGGTTT TTAAGCCCAG TCAACTCCAC
    CGAGCACATA GTGCTCTTTT GTTAAAATAT TCAAAAAAAT CTGAACTGTG GACGGCCCAG
    GAAACTATTG TCTATTTGGG GGACTACTTG ACTGTGAAGA AAAAAGGCAG ACAAAGAAAT
    GCTTTTTGGG TTCATCATCT TCATCAAGAA GAAATTCTGG GGAGGTATGT TGGGAAAGAC
    TATAAGGAGC AGAAGGGGCT CTGGCACCAC TTCACTGATG TGGAGCGACA GATGACCGCA
    CAGCACTATG TGACAGAATT TAACAAGAGA CTCTATGAAC AAAACATTCC CACCCAGATA
    TTCTACATCC CATCCACAAT ACTACTGATT TTAGAGGACA AGACAATAAA GGGATGTATC
    AGTGTGGAGC CTTACATACT GGGAGAATTT GTAAAATTGT CAAATAACAC GAAAGTGGTG
    AAAACAGAAT ACAAAGCCAC AGAATATGGC TTGGCCTATG GCCATTTTTC TTATGAGTTT
    TCTAATCATA GAGATGTTGT GGTCGATTTA CAAGGTTGGG TAACCGGTAA TGGAAAAGGA
    CTCATCTACC TCACAGATCC CCAGATTCAC TCCGTTGATC AGAAAGTTTT CACTACCAAT
    TTTGGAAAGA GAGGAATTTT TTACTTCTTT AATAACCAGC ATGTGGAATG TAATGAAATC
    TGCCATCGTC TTTCTTTGAC TAGACCTTCA ATGGAGAAAC CATGTAAGTC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016864140.1
    CDS817..3930
    Translation

    Target ORF information:

    RefSeq Version XM_017008651.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017008651.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTGGTACG AAGCAGCAGA GTTAATATGG GCCTCCATTG TAGGATATTT GGCACTTCCT 
    CAGCCGGATA AAAAGGGCCT CTCCACGTCG CTAGGTATAC TGGCAGACAT CTTTGTTTCC
    ATGAGCAAGA ACGATTATGA AAAGTTTAAA AACAATCCAC AAATTAATTT GAGCCTGCTG
    AAGGAGTTTG ACCACCATTT GCTGTCCGCT GCAGAAGCCT GCAAGCTGGC AGCTGCCTTC
    AGTGCCTATA CGCCGCTCTT CGTGCTCACA GCTGTGAATA TCCGTGGCAC GTGTTTATTG
    TCCTACAGTA GTTCAAATGA CTGTCCTCCA GAATTGAAAA ACTTACATCT GTGTGAAGCC
    AAAGAGGCCT TTGAGATTGG CCTCCTCACC AAGAGAGATG ATGAGCCTGT TACTGGAAAA
    CAGGAGCTTC ACAGCTTTGT CAAAGCTGCT TTCGGTCTCA CCACAGTGCA CAGAAGGCTC
    CATGGGGAGA CAGGGACGGT CCATGCAGCA AGTCAGCTCT GTAAGGAAGC AATGGGGAAG
    CTGTACAATT TCAGCACTTC CTCCAGAAGT CAGGACAGAG AAGCTCTGTC TCAAGAAGTT
    ATGTCTGTGA TTGCCCAGGT GAAGGAACAT TTACAAGTTC AAAGCTTCTC AAATGTAGAT
    GACAGATCTT ATGTTCCCGA GAGTTTCGAG TGCAGGTTGG ATAAACTTAT CTTGCATGGG
    CAAGGGGATT TCCAAAAAAT CCTTGACACC TATTCACAGC ACCATACTTC GGTGTGTGAA
    GTATTTGAAA GTGATTGTGG AAACAACAAA AATGAACAGA AAGATGCAAA AACAGGAGTC
    TGCATCACTG CTCTAAAAAC AGAAATAAAA AACATAGATA CTGTGAGTAC TACTCAAGAA
    AAGCCACATT GTCAAAGAGA CACAGGAATA TCTTCCTCCC TAATGGGTAA GAATGTTCAG
    AGGGAACTCA GAAGGGGAGG AAGGAGAAAC TGGACCCATT CTGATGCATT TCGAGTCTCC
    TTGGATCAAG ATGTGGAGAC TGAGACTGAG CCATCGGACT ACAGCAATGG TGAGGGAGCT
    GTTTTCAACA AGTCTCTGAG TGGCAGCCAG ACTTCCAGTG CTTGGAGCAA CTTATCAGGG
    TTTAGTTCCT CTGCAAGCTG GGAGGAAGTG AATTATCACG TTGACGACAG GTCAGCCAGA
    AAAGAGCCTG GCAAAGAACA TCTGGTGGAC ACTCAGTGTT CCACTGCCTT GTCTGAGGAG
    CTAGAGAATG ACAGGGAAGG CAGAGCTATG CATTCATTGC ATTCACAGCT TCATGATCTC
    TCTCTTCAGG AACCCAACAA TGACAATTTG GAGCCTTCTC AAAATCAGCC ACAGCAACAG
    ATGCCCTTGA CACCCTTCTC GCCTCATAAT ACCCCAGGCA TTTTCTTGGC CCCTGGTGCA
    GGGCTTCTAG AAGGAGCTCC AGAAGGTATC CAGGAAGTCA GAAATATGGG ACCCAGAAAT
    ACTTCTGCTC ACTCCAGACC CTCATATCGT TCTGCTTCTT GGTCTTCTGA TTCTGGTAGG
    CCCAAGAATA TGGGCACACA TCCTTCAGTC CAAAAAGAAG AAGCCTTTGA AATAATTGTT
    GAGTTTCCAG AAACCAACTG CGATGTCAAA GACAGGCAGG GGAAAGAGCA GGGAGAAGAA
    ATTAGTGAAA GAGGCGCAGG CCCTACATTT AAAGCTAGTC CCTCCTGGGT TGACCCAGAA
    GGAGAAACAG CAGAAAGCAC TGAAGATGCA CCCTTAGACT TTCACAGGGT CCTGCACAAT
    TCTCTGGGAA ACATTTCCAT GCTGCCATGT AGCTCCTTCA CCCCTAATTG GCCTGTTCAA
    AATCCTGACT CCAGAAAAAG TGGTGGCCCA GTCGCAGAGC AGGGCATCGA CCCTGATGCC
    TCCACAGTGG ATGAGGAGGG GCAACTGCTC GACAGCATGG ATGTTCCCTG CACAAATGGG
    CACGGCTCTC ATAGACTGTG CATTCTGAGA CAGCCGCCTG GTCAGAGGGC GGAGACCCCC
    AATTCCTCTG TAAGCGGTAA CATCCTCTTC CCTGTCCTCA GCGAGGACTG CACTACCACA
    GAGGAAGGAA ATCAGCCTGG AAACATGCTA AACTGCAGCC AGAACTCCAG CTCATCCTCA
    GTGTGGTGGC TGAAATCACC TGCATTTTCC AGTGGTTCTT CTGAGGGGGA CAGCCCTTGG
    TCCTATCTGA ATTCCAGTGG GAGTTCTTGG GTTTCATTGC CGGGAAAGAT GAGGAAAGAG
    ATCCTTGAGG CTCGCACCTT GCAACCTGAT GACTTTGAAA AGCTGTTGGC AGGAGTGAGG
    CATGATTGGC TGTTTCAGAG ACTAGAGAAT ACGGGGGTTT TTAAGCCCAG TCAACTCCAC
    CGAGCACATA GTGCTCTTTT GTTAAAATAT TCAAAAAAAT CTGAACTGTG GACGGCCCAG
    GAAACTATTG TCTATTTGGG GGACTACTTG ACTGTGAAGA AAAAAGGCAG ACAAAGAAAT
    GCTTTTTGGG TTCATCATCT TCATCAAGAA GAAATTCTGG GGAGGTATGT TGGGAAAGAC
    TATAAGGAGC AGAAGGGGCT CTGGCACCAC TTCACTGATG TGGAGCGACA GATGACCGCA
    CAGCACTATG TGACAGAATT TAACAAGAGA CTCTATGAAC AAAACATTCC CACCCAGATA
    TTCTACATCC CATCCACAAT ACTACTGATT TTAGAGGACA AGACAATAAA GGGATGTATC
    AGTGTGGAGC CTTACATACT GGGAGAATTT GTAAAATTGT CAAATAACAC GAAAGTGGTG
    AAAACAGAAT ACAAAGCCAC AGAATATGGC TTGGCCTATG GCCATTTTTC TTATGAGTTT
    TCTAATCATA GAGATGTTGT GGTCGATTTA CAAGGTTGGG TAACCGGTAA TGGAAAAGGA
    CTCATCTACC TCACAGATCC CCAGATTCAC TCCGTTGATC AGAAAGTTTT CACTACCAAT
    TTTGGAAAGA GAGGAATTTT TTACTTCTTT AATAACCAGC ATGTGGAATG TAATGAAATC
    TGCCATCGTC TTTCTTTGAC TAGACCTTCA ATGGAGAAAC CATGTAAGTC ATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14756
    Clone ID Related Accession (Same CDS sequence) NM_001253884.1
    Accession Version NM_001253884.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3501bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-27
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC004049.1, AK304708.1 and AK026715.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The resulting protein (isoform 2) is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304708.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2144335 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTGTCGGA AGAGGACAAG AGCGAGGACC AGCGCTGCAG AGGCGTCCCT GAGGGCCTCC 
    ATCCTCGCTC GGGACTGTGC GGCTGCGGCG GCTATTGTGT TCTTGGTGGA CCGGTTCCTG
    TATGGGCTCG ACGTCTCTGG AAAACTTCTG CAGGTCGCCA AAGGTCTCCA CAAGTTGCAG
    CCAGCCACGC CAATTGCCCC GCAGGTGGTT ATTCGCCAAG CCCGAATCTC CGTGAACTCA
    GGAAAACTTT TAAAAGCAGA GTATATTCTG AGCAGTCTAA TAAGCAACAA TGGAGCAACG
    GGTACCTGGC TGTACAGAAA TGAAAGTGAC AAGGTCCTGG TGCAGTCGGT CTGTATACAG
    ATCAGAGGGC AGATTCTGCA AAAGCTGGGG ATGTGGTACG AAGCAGCAGA GTTAATATGG
    GCCTCCATTG TAGGATATTT GGCACTTCCT CAGCCGGATA AAAAGGGCCT CTCCACGTCG
    CTAGGTATAC TGGCAGACAT CTTTGTTTCC ATGAGCAAGA ACGATTATGA AAAGTTTAAA
    AACAATCCAC AAATTAATTT GAGCCTGCTG AAGGAGTTTG ACCACCATTT GCTGTCCGCT
    GCAGAAGCCT GCAAGCTGGC AGCTGCCTTC AGTGCCTATA CGCCGCTCTT CGTGCTCACA
    GCTGTGAATA TCCGTGGCAC GTGTTTATTG TCCTACAGTA GTTCAAATGA CTGTCCTCCA
    GAATTGAAAA ACTTACATCT GTGTGAAGCC AAAGAGGCCT TTGAGATTGG CCTCCTCACC
    AAGAGAGATG ATGAGCCTGT TACTGGAAAA CAGGAGCTTC ACAGCTTTGT CAAAGCTGCT
    TTCGGTCTCA CCACAGTGCA CAGAAGGCTC CATGGGGAGA CAGGGACGGT CCATGCAGCA
    AGTCAGCTCT GTAAGGAAGC AATGGGGAAG CTGTACAATT TCAGCACTTC CTCCAGAAGT
    CAGGACAGAG AAGCTCTGTC TCAAGAAGTT ATGTCTGTGA TTGCCCAGGT GAAGGAACAT
    TTACAAGTTC AAAGCTTCTC AAATGTAGAT GACAGATCTT ATGTTCCCGA GAGTTTCGAG
    TGCAGGTTGG ATAAACTTAT CTTGCATGGG CAAGGGGATT TCCAAAAAAT CCTTGACACC
    TATTCACAGC ACCATACTTC GGTGTGTGAA GTATTTGAAA GTGATTGTGG AAACAACAAA
    AATGAACAGA AAGATGCAAA AACAGGAGTC TGCATCACTG CTCTAAAAAC AGAAATAAAA
    AACATAGATA CTGTGAGTAC TACTCAAGAA AAGCCACATT GTCAAAGAGA CACAGGAATA
    TCTTCCTCCC TAATGGGTAA GAATGTTCAG AGGGAACTCA GAAGGGGAGG AAGGAGAAAC
    TGGACCCATT CTGATGCATT TCGAGTCTCC TTGGATCAAG ATGTGGAGAC TGAGACTGAG
    CCATCGGACT ACAGCAATGG TGAGGGAGCT GTTTTCAACA AGTCTCTGAG TGGCAGCCAG
    ACTTCCAGTG CTTGGAGCAA CTTATCAGGG TTTAGTTCCT CTGCAAGCTG GGAGGAAGTG
    AATTATCACG TTGACGACAG GTCAGCCAGA AAAGAGCCTG GCAAAGAACA TCTGGTGGAC
    ACTCAGTGTT CCACTGCCTT GTCTGAGGAG CTAGAGAATG ACAGGGAAGG CAGAGCTATG
    CATTCATTGC ATTCACAGCT TCATGATCTC TCTCTTCAGG AACCCAACAA TGACAATTTG
    GAGCCTTCTC AAAATCAGCC ACAGCAACAG ATGCCCTTGA CACCCTTCTC GCCTCATAAT
    ACCCCAGGCA TTTTCTTGGC CCCTGGTGCA GGGCTTCTAG AAGGAGCTCC AGAAGGTATC
    CAGGAAGTCA GAAATATGGG ACCCAGAAAT ACTTCTGCTC ACTCCAGACC CTCATATCGT
    TCTGCTTCTT GGTCTTCTGA TTCTGGTAGG CCCAAGAATA TGGGCACACA TCCTTCAGTC
    CAAAAAGAAG AAGCCTTTGA AATAATTGTT GAGTTTCCAG AAACCAACTG CGATGTCAAA
    GACAGGCAGG GGAAAGAGCA GGGAGAAGAA ATTAGTGAAA GAGGCGCAGG CCCTACATTT
    AAAGCTAGTC CCTCCTGGGT TGACCCAGAA GGAGAAACAG CAGAAAGCAC TGAAGATGCA
    CCCTTAGACT TTCACAGGGT CCTGCACAAT TCTCTGGGAA ACATTTCCAT GCTGCCATGT
    AGCTCCTTCA CCCCTAATTG GCCTGTTCAA AATCCTGACT CCAGAAAAAG TGGTGGCCCA
    GTCGCAGAGC AGGGCATCGA CCCTGATGCC TCCACAGTGG ATGAGGAGGG GCAACTGCTC
    GACAGCATGG ATGTTCCCTG CACAAATGGG CACGGCTCTC ATAGACTGTG CATTCTGAGA
    CAGCCGCCTG GTCAGAGGGC GGAGACCCCC AATTCCTCTG TAAGCGGTAA CATCCTCTTC
    CCTGTCCTCA GCGAGGACTG CACTACCACA GAGGAAGGAA ATCAGCCTGG AAACATGCTA
    AACTGCAGCC AGAACTCCAG CTCATCCTCA GTGTGGTGGC TGAAATCACC TGCATTTTCC
    AGTGGTTCTT CTGAGGGGGA CAGCCCTTGG TCCTATCTGA ATTCCAGTGG GAGTTCTTGG
    GTTTCATTGC CGGGAAAGAT GAGGAAAGAG ATCCTTGAGG CTCGCACCTT GCAACCTGAT
    GACTTTGAAA AGCTGTTGGC AGGAGTGAGG CATGATTGGC TGTTTCAGAG ACTAGAGAAT
    ACGGGGGTTT TTAAGCCCAG TCAACTCCAC CGAGCACATA GTGCTCTTTT GTTAAAATAT
    TCAAAAAAAT CTGAACTGTG GACGGCCCAG GAAACTATTG TCTATTTGGG GGACTACTTG
    ACTGTGAAGA AAAAAGGCAG ACAAAGAAAT GCTTTTTGGG TTCATCATCT TCATCAAGAA
    GAAATTCTGG GGAGGTATGT TGGGAAAGAC TATAAGGAGC AGAAGGGGCT CTGGCACCAC
    TTCACTGATG TGGAGCGACA GATGACCGCA CAGCACTATG TGACAGAATT TAACAAGAGA
    CTCTATGAAC AAAACATTCC CACCCAGATA TTCTACATCC CATCCACAAT ACTACTGATT
    TTAGAGGACA AGACAATAAA GGGATGTATC AGTGTGGAGC CTTACATACT GGGAGAATTT
    GTAAAATTGT CAAATAACAC GAAAGTGGTG AAAACAGAAT ACAAAGCCAC AGAATATGGC
    TTGGCCTATG GCCATTTTTC TTATGAGTTT TCTAATCATA GAGATGTTGT GGTCGATTTA
    CAAGGTTGGG TAACCGGTAA TGGAAAAGGA CTCATCTACC TCACAGATCC CCAGATTCAC
    TCCGTTGATC AGAAAGTTTT CACTACCAAT TTTGGAAAGA GAGGAATTTT TTACTTCTTT
    AATAACCAGC ATGTGGAATG TAATGAAATC TGCCATCGTC TTTCTTTGAC TAGACCTTCA
    ATGGAGAAAC CATGCACATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001240813.1
    CDS307..3807
    Translation

    Target ORF information:

    RefSeq Version NM_001253884.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001253884.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGTGTCGGA AGAGGACAAG AGCGAGGACC AGCGCTGCAG AGGCGTCCCT GAGGGCCTCC 
    ATCCTCGCTC GGGACTGTGC GGCTGCGGCG GCTATTGTGT TCTTGGTGGA CCGGTTCCTG
    TATGGGCTCG ACGTCTCTGG AAAACTTCTG CAGGTCGCCA AAGGTCTCCA CAAGTTGCAG
    CCAGCCACGC CAATTGCCCC GCAGGTGGTT ATTCGCCAAG CCCGAATCTC CGTGAACTCA
    GGAAAACTTT TAAAAGCAGA GTATATTCTG AGCAGTCTAA TAAGCAACAA TGGAGCAACG
    GGTACCTGGC TGTACAGAAA TGAAAGTGAC AAGGTCCTGG TGCAGTCGGT CTGTATACAG
    ATCAGAGGGC AGATTCTGCA AAAGCTGGGG ATGTGGTACG AAGCAGCAGA GTTAATATGG
    GCCTCCATTG TAGGATATTT GGCACTTCCT CAGCCGGATA AAAAGGGCCT CTCCACGTCG
    CTAGGTATAC TGGCAGACAT CTTTGTTTCC ATGAGCAAGA ACGATTATGA AAAGTTTAAA
    AACAATCCAC AAATTAATTT GAGCCTGCTG AAGGAGTTTG ACCACCATTT GCTGTCCGCT
    GCAGAAGCCT GCAAGCTGGC AGCTGCCTTC AGTGCCTATA CGCCGCTCTT CGTGCTCACA
    GCTGTGAATA TCCGTGGCAC GTGTTTATTG TCCTACAGTA GTTCAAATGA CTGTCCTCCA
    GAATTGAAAA ACTTACATCT GTGTGAAGCC AAAGAGGCCT TTGAGATTGG CCTCCTCACC
    AAGAGAGATG ATGAGCCTGT TACTGGAAAA CAGGAGCTTC ACAGCTTTGT CAAAGCTGCT
    TTCGGTCTCA CCACAGTGCA CAGAAGGCTC CATGGGGAGA CAGGGACGGT CCATGCAGCA
    AGTCAGCTCT GTAAGGAAGC AATGGGGAAG CTGTACAATT TCAGCACTTC CTCCAGAAGT
    CAGGACAGAG AAGCTCTGTC TCAAGAAGTT ATGTCTGTGA TTGCCCAGGT GAAGGAACAT
    TTACAAGTTC AAAGCTTCTC AAATGTAGAT GACAGATCTT ATGTTCCCGA GAGTTTCGAG
    TGCAGGTTGG ATAAACTTAT CTTGCATGGG CAAGGGGATT TCCAAAAAAT CCTTGACACC
    TATTCACAGC ACCATACTTC GGTGTGTGAA GTATTTGAAA GTGATTGTGG AAACAACAAA
    AATGAACAGA AAGATGCAAA AACAGGAGTC TGCATCACTG CTCTAAAAAC AGAAATAAAA
    AACATAGATA CTGTGAGTAC TACTCAAGAA AAGCCACATT GTCAAAGAGA CACAGGAATA
    TCTTCCTCCC TAATGGGTAA GAATGTTCAG AGGGAACTCA GAAGGGGAGG AAGGAGAAAC
    TGGACCCATT CTGATGCATT TCGAGTCTCC TTGGATCAAG ATGTGGAGAC TGAGACTGAG
    CCATCGGACT ACAGCAATGG TGAGGGAGCT GTTTTCAACA AGTCTCTGAG TGGCAGCCAG
    ACTTCCAGTG CTTGGAGCAA CTTATCAGGG TTTAGTTCCT CTGCAAGCTG GGAGGAAGTG
    AATTATCACG TTGACGACAG GTCAGCCAGA AAAGAGCCTG GCAAAGAACA TCTGGTGGAC
    ACTCAGTGTT CCACTGCCTT GTCTGAGGAG CTAGAGAATG ACAGGGAAGG CAGAGCTATG
    CATTCATTGC ATTCACAGCT TCATGATCTC TCTCTTCAGG AACCCAACAA TGACAATTTG
    GAGCCTTCTC AAAATCAGCC ACAGCAACAG ATGCCCTTGA CACCCTTCTC GCCTCATAAT
    ACCCCAGGCA TTTTCTTGGC CCCTGGTGCA GGGCTTCTAG AAGGAGCTCC AGAAGGTATC
    CAGGAAGTCA GAAATATGGG ACCCAGAAAT ACTTCTGCTC ACTCCAGACC CTCATATCGT
    TCTGCTTCTT GGTCTTCTGA TTCTGGTAGG CCCAAGAATA TGGGCACACA TCCTTCAGTC
    CAAAAAGAAG AAGCCTTTGA AATAATTGTT GAGTTTCCAG AAACCAACTG CGATGTCAAA
    GACAGGCAGG GGAAAGAGCA GGGAGAAGAA ATTAGTGAAA GAGGCGCAGG CCCTACATTT
    AAAGCTAGTC CCTCCTGGGT TGACCCAGAA GGAGAAACAG CAGAAAGCAC TGAAGATGCA
    CCCTTAGACT TTCACAGGGT CCTGCACAAT TCTCTGGGAA ACATTTCCAT GCTGCCATGT
    AGCTCCTTCA CCCCTAATTG GCCTGTTCAA AATCCTGACT CCAGAAAAAG TGGTGGCCCA
    GTCGCAGAGC AGGGCATCGA CCCTGATGCC TCCACAGTGG ATGAGGAGGG GCAACTGCTC
    GACAGCATGG ATGTTCCCTG CACAAATGGG CACGGCTCTC ATAGACTGTG CATTCTGAGA
    CAGCCGCCTG GTCAGAGGGC GGAGACCCCC AATTCCTCTG TAAGCGGTAA CATCCTCTTC
    CCTGTCCTCA GCGAGGACTG CACTACCACA GAGGAAGGAA ATCAGCCTGG AAACATGCTA
    AACTGCAGCC AGAACTCCAG CTCATCCTCA GTGTGGTGGC TGAAATCACC TGCATTTTCC
    AGTGGTTCTT CTGAGGGGGA CAGCCCTTGG TCCTATCTGA ATTCCAGTGG GAGTTCTTGG
    GTTTCATTGC CGGGAAAGAT GAGGAAAGAG ATCCTTGAGG CTCGCACCTT GCAACCTGAT
    GACTTTGAAA AGCTGTTGGC AGGAGTGAGG CATGATTGGC TGTTTCAGAG ACTAGAGAAT
    ACGGGGGTTT TTAAGCCCAG TCAACTCCAC CGAGCACATA GTGCTCTTTT GTTAAAATAT
    TCAAAAAAAT CTGAACTGTG GACGGCCCAG GAAACTATTG TCTATTTGGG GGACTACTTG
    ACTGTGAAGA AAAAAGGCAG ACAAAGAAAT GCTTTTTGGG TTCATCATCT TCATCAAGAA
    GAAATTCTGG GGAGGTATGT TGGGAAAGAC TATAAGGAGC AGAAGGGGCT CTGGCACCAC
    TTCACTGATG TGGAGCGACA GATGACCGCA CAGCACTATG TGACAGAATT TAACAAGAGA
    CTCTATGAAC AAAACATTCC CACCCAGATA TTCTACATCC CATCCACAAT ACTACTGATT
    TTAGAGGACA AGACAATAAA GGGATGTATC AGTGTGGAGC CTTACATACT GGGAGAATTT
    GTAAAATTGT CAAATAACAC GAAAGTGGTG AAAACAGAAT ACAAAGCCAC AGAATATGGC
    TTGGCCTATG GCCATTTTTC TTATGAGTTT TCTAATCATA GAGATGTTGT GGTCGATTTA
    CAAGGTTGGG TAACCGGTAA TGGAAAAGGA CTCATCTACC TCACAGATCC CCAGATTCAC
    TCCGTTGATC AGAAAGTTTT CACTACCAAT TTTGGAAAGA GAGGAATTTT TTACTTCTTT
    AATAACCAGC ATGTGGAATG TAATGAAATC TGCCATCGTC TTTCTTTGAC TAGACCTTCA
    ATGGAGAAAC CATGCACATA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39100
    Clone ID Related Accession (Same CDS sequence) XM_005263245.4 , XM_005263246.4
    Accession Version XM_005263245.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3738bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263245.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGAGCCT GCTGAAGGAG TTTGACCACC ATTTGCTGTC CGCTGCAGAA
    GCCTGCAAGC TGGCAGCTGC CTTCAGTGCC TATACGCCGC TCTTCGTGCT CACAGCTGTG
    AATATCCGTG GCACGTGTTT ATTGTCCTAC AGTAGTTCAA ATGACTGTCC TCCAGAATTG
    AAAAACTTAC ATCTGTGTGA AGCCAAAGAG GCCTTTGAGA TTGGCCTCCT CACCAAGAGA
    GATGATGAGC CTGTTACTGG AAAACAGGAG CTTCACAGCT TTGTCAAAGC TGCTTTCGGT
    CTCACCACAG TGCACAGAAG GCTCCATGGG GAGACAGGGA CGGTCCATGC AGCAAGTCAG
    CTCTGTAAGG AAGCAATGGG GAAGCTGTAC AATTTCAGCA CTTCCTCCAG AAGTCAGGAC
    AGAGAAGCTC TGTCTCAAGA AGTTATGTCT GTGATTGCCC AGGTGAAGGA ACATTTACAA
    GTTCAAAGCT TCTCAAATGT AGATGACAGA TCTTATGTTC CCGAGAGTTT CGAGTGCAGG
    TTGGATAAAC TTATCTTGCA TGGGCAAGGG GATTTCCAAA AAATCCTTGA CACCTATTCA
    CAGCACCATA CTTCGGTGTG TGAAGTATTT GAAAGTGATT GTGGAAACAA CAAAAATGAA
    CAGAAAGATG CAAAAACAGG AGTCTGCATC ACTGCTCTAA AAACAGAAAT AAAAAACATA
    GATACTGTGA GTACTACTCA AGAAAAGCCA CATTGTCAAA GAGACACAGG AATATCTTCC
    TCCCTAATGG GTAAGAATGT TCAGAGGGAA CTCAGAAGGG GAGGAAGGAG AAACTGGACC
    CATTCTGATG CATTTCGAGT CTCCTTGGAT CAAGATGTGG AGACTGAGAC TGAGCCATCG
    GACTACAGCA ATGGTGAGGG AGCTGTTTTC AACAAGTCTC TGAGTGGCAG CCAGACTTCC
    AGTGCTTGGA GCAACTTATC AGGGTTTAGT TCCTCTGCAA GCTGGGAGGA AGTGAATTAT
    CACGTTGACG ACAGGTCAGC CAGAAAAGAG CCTGGCAAAG AACATCTGGT GGACACTCAG
    TGTTCCACTG CCTTGTCTGA GGAGCTAGAG AATGACAGGG AAGGCAGAGC TATGCATTCA
    TTGCATTCAC AGCTTCATGA TCTCTCTCTT CAGGAACCCA ACAATGACAA TTTGGAGCCT
    TCTCAAAATC AGCCACAGCA ACAGATGCCC TTGACACCCT TCTCGCCTCA TAATACCCCA
    GGCATTTTCT TGGCCCCTGG TGCAGGGCTT CTAGAAGGAG CTCCAGAAGG TATCCAGGAA
    GTCAGAAATA TGGGACCCAG AAATACTTCT GCTCACTCCA GACCCTCATA TCGTTCTGCT
    TCTTGGTCTT CTGATTCTGG TAGGCCCAAG AATATGGGCA CACATCCTTC AGTCCAAAAA
    GAAGAAGCCT TTGAAATAAT TGTTGAGTTT CCAGAAACCA ACTGCGATGT CAAAGACAGG
    CAGGGGAAAG AGCAGGGAGA AGAAATTAGT GAAAGAGGCG CAGGCCCTAC ATTTAAAGCT
    AGTCCCTCCT GGGTTGACCC AGAAGGAGAA ACAGCAGAAA GCACTGAAGA TGCACCCTTA
    GACTTTCACA GGGTCCTGCA CAATTCTCTG GGAAACATTT CCATGCTGCC ATGTAGCTCC
    TTCACCCCTA ATTGGCCTGT TCAAAATCCT GACTCCAGAA AAAGTGGTGG CCCAGTCGCA
    GAGCAGGGCA TCGACCCTGA TGCCTCCACA GTGGATGAGG AGGGGCAACT GCTCGACAGC
    ATGGATGTTC CCTGCACAAA TGGGCACGGC TCTCATAGAC TGTGCATTCT GAGACAGCCG
    CCTGGTCAGA GGGCGGAGAC CCCCAATTCC TCTGTAAGCG GTAACATCCT CTTCCCTGTC
    CTCAGCGAGG ACTGCACTAC CACAGAGGAA GGAAATCAGC CTGGAAACAT GCTAAACTGC
    AGCCAGAACT CCAGCTCATC CTCAGTGTGG TGGCTGAAAT CACCTGCATT TTCCAGTGGT
    TCTTCTGAGG GGGACAGCCC TTGGTCCTAT CTGAATTCCA GTGGGAGTTC TTGGGTTTCA
    TTGCCGGGAA AGATGAGGAA AGAGATCCTT GAGGCTCGCA CCTTGCAACC TGATGACTTT
    GAAAAGCTGT TGGCAGGAGT GAGGCATGAT TGGCTGTTTC AGAGACTAGA GAATACGGGG
    GTTTTTAAGC CCAGTCAACT CCACCGAGCA CATAGTGCTC TTTTGTTAAA ATATTCAAAA
    AAATCTGAAC TGTGGACGGC CCAGGAAACT ATTGTCTATT TGGGGGACTA CTTGACTGTG
    AAGAAAAAAG GCAGACAAAG AAATGCTTTT TGGGTTCATC ATCTTCATCA AGAAGAAATT
    CTGGGGAGGT ATGTTGGGAA AGACTATAAG GAGCAGAAGG GGCTCTGGCA CCACTTCACT
    GATGTGGAGC GACAGATGAC CGCACAGCAC TATGTGACAG AATTTAACAA GAGACTCTAT
    GAACAAAACA TTCCCACCCA GATATTCTAC ATCCCATCCA CAATACTACT GATTTTAGAG
    GACAAGACAA TAAAGGGATG TATCAGTGTG GAGCCTTACA TACTGGGAGA ATTTGTAAAA
    TTGTCAAATA ACACGAAAGT GGTGAAAACA GAATACAAAG CCACAGAATA TGGCTTGGCC
    TATGGCCATT TTTCTTATGA GTTTTCTAAT CATAGAGATG TTGTGGTCGA TTTACAAGGT
    TGGGTAACCG GTAATGGAAA AGGACTCATC TACCTCACAG ATCCCCAGAT TCACTCCGTT
    GATCAGAAAG TTTTCACTAC CAATTTTGGA AAGAGAGGAA TTTTTTACTT CTTTAATAAC
    CAGCATGTGG AATGTAATGA AATCTGCCAT CGTCTTTCTT TGACTAGACC TTCAATGGAG
    AAACCATGTA AGTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005263302.1
    CDS280..4017
    Translation

    Target ORF information:

    RefSeq Version XM_005263245.4
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005263245.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGAGCCT GCTGAAGGAG TTTGACCACC ATTTGCTGTC CGCTGCAGAA
    GCCTGCAAGC TGGCAGCTGC CTTCAGTGCC TATACGCCGC TCTTCGTGCT CACAGCTGTG
    AATATCCGTG GCACGTGTTT ATTGTCCTAC AGTAGTTCAA ATGACTGTCC TCCAGAATTG
    AAAAACTTAC ATCTGTGTGA AGCCAAAGAG GCCTTTGAGA TTGGCCTCCT CACCAAGAGA
    GATGATGAGC CTGTTACTGG AAAACAGGAG CTTCACAGCT TTGTCAAAGC TGCTTTCGGT
    CTCACCACAG TGCACAGAAG GCTCCATGGG GAGACAGGGA CGGTCCATGC AGCAAGTCAG
    CTCTGTAAGG AAGCAATGGG GAAGCTGTAC AATTTCAGCA CTTCCTCCAG AAGTCAGGAC
    AGAGAAGCTC TGTCTCAAGA AGTTATGTCT GTGATTGCCC AGGTGAAGGA ACATTTACAA
    GTTCAAAGCT TCTCAAATGT AGATGACAGA TCTTATGTTC CCGAGAGTTT CGAGTGCAGG
    TTGGATAAAC TTATCTTGCA TGGGCAAGGG GATTTCCAAA AAATCCTTGA CACCTATTCA
    CAGCACCATA CTTCGGTGTG TGAAGTATTT GAAAGTGATT GTGGAAACAA CAAAAATGAA
    CAGAAAGATG CAAAAACAGG AGTCTGCATC ACTGCTCTAA AAACAGAAAT AAAAAACATA
    GATACTGTGA GTACTACTCA AGAAAAGCCA CATTGTCAAA GAGACACAGG AATATCTTCC
    TCCCTAATGG GTAAGAATGT TCAGAGGGAA CTCAGAAGGG GAGGAAGGAG AAACTGGACC
    CATTCTGATG CATTTCGAGT CTCCTTGGAT CAAGATGTGG AGACTGAGAC TGAGCCATCG
    GACTACAGCA ATGGTGAGGG AGCTGTTTTC AACAAGTCTC TGAGTGGCAG CCAGACTTCC
    AGTGCTTGGA GCAACTTATC AGGGTTTAGT TCCTCTGCAA GCTGGGAGGA AGTGAATTAT
    CACGTTGACG ACAGGTCAGC CAGAAAAGAG CCTGGCAAAG AACATCTGGT GGACACTCAG
    TGTTCCACTG CCTTGTCTGA GGAGCTAGAG AATGACAGGG AAGGCAGAGC TATGCATTCA
    TTGCATTCAC AGCTTCATGA TCTCTCTCTT CAGGAACCCA ACAATGACAA TTTGGAGCCT
    TCTCAAAATC AGCCACAGCA ACAGATGCCC TTGACACCCT TCTCGCCTCA TAATACCCCA
    GGCATTTTCT TGGCCCCTGG TGCAGGGCTT CTAGAAGGAG CTCCAGAAGG TATCCAGGAA
    GTCAGAAATA TGGGACCCAG AAATACTTCT GCTCACTCCA GACCCTCATA TCGTTCTGCT
    TCTTGGTCTT CTGATTCTGG TAGGCCCAAG AATATGGGCA CACATCCTTC AGTCCAAAAA
    GAAGAAGCCT TTGAAATAAT TGTTGAGTTT CCAGAAACCA ACTGCGATGT CAAAGACAGG
    CAGGGGAAAG AGCAGGGAGA AGAAATTAGT GAAAGAGGCG CAGGCCCTAC ATTTAAAGCT
    AGTCCCTCCT GGGTTGACCC AGAAGGAGAA ACAGCAGAAA GCACTGAAGA TGCACCCTTA
    GACTTTCACA GGGTCCTGCA CAATTCTCTG GGAAACATTT CCATGCTGCC ATGTAGCTCC
    TTCACCCCTA ATTGGCCTGT TCAAAATCCT GACTCCAGAA AAAGTGGTGG CCCAGTCGCA
    GAGCAGGGCA TCGACCCTGA TGCCTCCACA GTGGATGAGG AGGGGCAACT GCTCGACAGC
    ATGGATGTTC CCTGCACAAA TGGGCACGGC TCTCATAGAC TGTGCATTCT GAGACAGCCG
    CCTGGTCAGA GGGCGGAGAC CCCCAATTCC TCTGTAAGCG GTAACATCCT CTTCCCTGTC
    CTCAGCGAGG ACTGCACTAC CACAGAGGAA GGAAATCAGC CTGGAAACAT GCTAAACTGC
    AGCCAGAACT CCAGCTCATC CTCAGTGTGG TGGCTGAAAT CACCTGCATT TTCCAGTGGT
    TCTTCTGAGG GGGACAGCCC TTGGTCCTAT CTGAATTCCA GTGGGAGTTC TTGGGTTTCA
    TTGCCGGGAA AGATGAGGAA AGAGATCCTT GAGGCTCGCA CCTTGCAACC TGATGACTTT
    GAAAAGCTGT TGGCAGGAGT GAGGCATGAT TGGCTGTTTC AGAGACTAGA GAATACGGGG
    GTTTTTAAGC CCAGTCAACT CCACCGAGCA CATAGTGCTC TTTTGTTAAA ATATTCAAAA
    AAATCTGAAC TGTGGACGGC CCAGGAAACT ATTGTCTATT TGGGGGACTA CTTGACTGTG
    AAGAAAAAAG GCAGACAAAG AAATGCTTTT TGGGTTCATC ATCTTCATCA AGAAGAAATT
    CTGGGGAGGT ATGTTGGGAA AGACTATAAG GAGCAGAAGG GGCTCTGGCA CCACTTCACT
    GATGTGGAGC GACAGATGAC CGCACAGCAC TATGTGACAG AATTTAACAA GAGACTCTAT
    GAACAAAACA TTCCCACCCA GATATTCTAC ATCCCATCCA CAATACTACT GATTTTAGAG
    GACAAGACAA TAAAGGGATG TATCAGTGTG GAGCCTTACA TACTGGGAGA ATTTGTAAAA
    TTGTCAAATA ACACGAAAGT GGTGAAAACA GAATACAAAG CCACAGAATA TGGCTTGGCC
    TATGGCCATT TTTCTTATGA GTTTTCTAAT CATAGAGATG TTGTGGTCGA TTTACAAGGT
    TGGGTAACCG GTAATGGAAA AGGACTCATC TACCTCACAG ATCCCCAGAT TCACTCCGTT
    GATCAGAAAG TTTTCACTAC CAATTTTGGA AAGAGAGGAA TTTTTTACTT CTTTAATAAC
    CAGCATGTGG AATGTAATGA AATCTGCCAT CGTCTTTCTT TGACTAGACC TTCAATGGAG
    AAACCATGTA AGTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39100
    Clone ID Related Accession (Same CDS sequence) XM_005263245.4 , XM_005263246.4
    Accession Version XM_005263246.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3738bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005263246.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGAGCCT GCTGAAGGAG TTTGACCACC ATTTGCTGTC CGCTGCAGAA
    GCCTGCAAGC TGGCAGCTGC CTTCAGTGCC TATACGCCGC TCTTCGTGCT CACAGCTGTG
    AATATCCGTG GCACGTGTTT ATTGTCCTAC AGTAGTTCAA ATGACTGTCC TCCAGAATTG
    AAAAACTTAC ATCTGTGTGA AGCCAAAGAG GCCTTTGAGA TTGGCCTCCT CACCAAGAGA
    GATGATGAGC CTGTTACTGG AAAACAGGAG CTTCACAGCT TTGTCAAAGC TGCTTTCGGT
    CTCACCACAG TGCACAGAAG GCTCCATGGG GAGACAGGGA CGGTCCATGC AGCAAGTCAG
    CTCTGTAAGG AAGCAATGGG GAAGCTGTAC AATTTCAGCA CTTCCTCCAG AAGTCAGGAC
    AGAGAAGCTC TGTCTCAAGA AGTTATGTCT GTGATTGCCC AGGTGAAGGA ACATTTACAA
    GTTCAAAGCT TCTCAAATGT AGATGACAGA TCTTATGTTC CCGAGAGTTT CGAGTGCAGG
    TTGGATAAAC TTATCTTGCA TGGGCAAGGG GATTTCCAAA AAATCCTTGA CACCTATTCA
    CAGCACCATA CTTCGGTGTG TGAAGTATTT GAAAGTGATT GTGGAAACAA CAAAAATGAA
    CAGAAAGATG CAAAAACAGG AGTCTGCATC ACTGCTCTAA AAACAGAAAT AAAAAACATA
    GATACTGTGA GTACTACTCA AGAAAAGCCA CATTGTCAAA GAGACACAGG AATATCTTCC
    TCCCTAATGG GTAAGAATGT TCAGAGGGAA CTCAGAAGGG GAGGAAGGAG AAACTGGACC
    CATTCTGATG CATTTCGAGT CTCCTTGGAT CAAGATGTGG AGACTGAGAC TGAGCCATCG
    GACTACAGCA ATGGTGAGGG AGCTGTTTTC AACAAGTCTC TGAGTGGCAG CCAGACTTCC
    AGTGCTTGGA GCAACTTATC AGGGTTTAGT TCCTCTGCAA GCTGGGAGGA AGTGAATTAT
    CACGTTGACG ACAGGTCAGC CAGAAAAGAG CCTGGCAAAG AACATCTGGT GGACACTCAG
    TGTTCCACTG CCTTGTCTGA GGAGCTAGAG AATGACAGGG AAGGCAGAGC TATGCATTCA
    TTGCATTCAC AGCTTCATGA TCTCTCTCTT CAGGAACCCA ACAATGACAA TTTGGAGCCT
    TCTCAAAATC AGCCACAGCA ACAGATGCCC TTGACACCCT TCTCGCCTCA TAATACCCCA
    GGCATTTTCT TGGCCCCTGG TGCAGGGCTT CTAGAAGGAG CTCCAGAAGG TATCCAGGAA
    GTCAGAAATA TGGGACCCAG AAATACTTCT GCTCACTCCA GACCCTCATA TCGTTCTGCT
    TCTTGGTCTT CTGATTCTGG TAGGCCCAAG AATATGGGCA CACATCCTTC AGTCCAAAAA
    GAAGAAGCCT TTGAAATAAT TGTTGAGTTT CCAGAAACCA ACTGCGATGT CAAAGACAGG
    CAGGGGAAAG AGCAGGGAGA AGAAATTAGT GAAAGAGGCG CAGGCCCTAC ATTTAAAGCT
    AGTCCCTCCT GGGTTGACCC AGAAGGAGAA ACAGCAGAAA GCACTGAAGA TGCACCCTTA
    GACTTTCACA GGGTCCTGCA CAATTCTCTG GGAAACATTT CCATGCTGCC ATGTAGCTCC
    TTCACCCCTA ATTGGCCTGT TCAAAATCCT GACTCCAGAA AAAGTGGTGG CCCAGTCGCA
    GAGCAGGGCA TCGACCCTGA TGCCTCCACA GTGGATGAGG AGGGGCAACT GCTCGACAGC
    ATGGATGTTC CCTGCACAAA TGGGCACGGC TCTCATAGAC TGTGCATTCT GAGACAGCCG
    CCTGGTCAGA GGGCGGAGAC CCCCAATTCC TCTGTAAGCG GTAACATCCT CTTCCCTGTC
    CTCAGCGAGG ACTGCACTAC CACAGAGGAA GGAAATCAGC CTGGAAACAT GCTAAACTGC
    AGCCAGAACT CCAGCTCATC CTCAGTGTGG TGGCTGAAAT CACCTGCATT TTCCAGTGGT
    TCTTCTGAGG GGGACAGCCC TTGGTCCTAT CTGAATTCCA GTGGGAGTTC TTGGGTTTCA
    TTGCCGGGAA AGATGAGGAA AGAGATCCTT GAGGCTCGCA CCTTGCAACC TGATGACTTT
    GAAAAGCTGT TGGCAGGAGT GAGGCATGAT TGGCTGTTTC AGAGACTAGA GAATACGGGG
    GTTTTTAAGC CCAGTCAACT CCACCGAGCA CATAGTGCTC TTTTGTTAAA ATATTCAAAA
    AAATCTGAAC TGTGGACGGC CCAGGAAACT ATTGTCTATT TGGGGGACTA CTTGACTGTG
    AAGAAAAAAG GCAGACAAAG AAATGCTTTT TGGGTTCATC ATCTTCATCA AGAAGAAATT
    CTGGGGAGGT ATGTTGGGAA AGACTATAAG GAGCAGAAGG GGCTCTGGCA CCACTTCACT
    GATGTGGAGC GACAGATGAC CGCACAGCAC TATGTGACAG AATTTAACAA GAGACTCTAT
    GAACAAAACA TTCCCACCCA GATATTCTAC ATCCCATCCA CAATACTACT GATTTTAGAG
    GACAAGACAA TAAAGGGATG TATCAGTGTG GAGCCTTACA TACTGGGAGA ATTTGTAAAA
    TTGTCAAATA ACACGAAAGT GGTGAAAACA GAATACAAAG CCACAGAATA TGGCTTGGCC
    TATGGCCATT TTTCTTATGA GTTTTCTAAT CATAGAGATG TTGTGGTCGA TTTACAAGGT
    TGGGTAACCG GTAATGGAAA AGGACTCATC TACCTCACAG ATCCCCAGAT TCACTCCGTT
    GATCAGAAAG TTTTCACTAC CAATTTTGGA AAGAGAGGAA TTTTTTACTT CTTTAATAAC
    CAGCATGTGG AATGTAATGA AATCTGCCAT CGTCTTTCTT TGACTAGACC TTCAATGGAG
    AAACCATGTA AGTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005263303.1
    CDS203..3940
    Translation

    Target ORF information:

    RefSeq Version XM_005263246.4
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005263246.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGAAAA
    CTTTTAAAAG CAGAGTATAT TCTGAGCAGT CTAATAAGCA ACAATGGAGC AACGGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGAGCCT GCTGAAGGAG TTTGACCACC ATTTGCTGTC CGCTGCAGAA
    GCCTGCAAGC TGGCAGCTGC CTTCAGTGCC TATACGCCGC TCTTCGTGCT CACAGCTGTG
    AATATCCGTG GCACGTGTTT ATTGTCCTAC AGTAGTTCAA ATGACTGTCC TCCAGAATTG
    AAAAACTTAC ATCTGTGTGA AGCCAAAGAG GCCTTTGAGA TTGGCCTCCT CACCAAGAGA
    GATGATGAGC CTGTTACTGG AAAACAGGAG CTTCACAGCT TTGTCAAAGC TGCTTTCGGT
    CTCACCACAG TGCACAGAAG GCTCCATGGG GAGACAGGGA CGGTCCATGC AGCAAGTCAG
    CTCTGTAAGG AAGCAATGGG GAAGCTGTAC AATTTCAGCA CTTCCTCCAG AAGTCAGGAC
    AGAGAAGCTC TGTCTCAAGA AGTTATGTCT GTGATTGCCC AGGTGAAGGA ACATTTACAA
    GTTCAAAGCT TCTCAAATGT AGATGACAGA TCTTATGTTC CCGAGAGTTT CGAGTGCAGG
    TTGGATAAAC TTATCTTGCA TGGGCAAGGG GATTTCCAAA AAATCCTTGA CACCTATTCA
    CAGCACCATA CTTCGGTGTG TGAAGTATTT GAAAGTGATT GTGGAAACAA CAAAAATGAA
    CAGAAAGATG CAAAAACAGG AGTCTGCATC ACTGCTCTAA AAACAGAAAT AAAAAACATA
    GATACTGTGA GTACTACTCA AGAAAAGCCA CATTGTCAAA GAGACACAGG AATATCTTCC
    TCCCTAATGG GTAAGAATGT TCAGAGGGAA CTCAGAAGGG GAGGAAGGAG AAACTGGACC
    CATTCTGATG CATTTCGAGT CTCCTTGGAT CAAGATGTGG AGACTGAGAC TGAGCCATCG
    GACTACAGCA ATGGTGAGGG AGCTGTTTTC AACAAGTCTC TGAGTGGCAG CCAGACTTCC
    AGTGCTTGGA GCAACTTATC AGGGTTTAGT TCCTCTGCAA GCTGGGAGGA AGTGAATTAT
    CACGTTGACG ACAGGTCAGC CAGAAAAGAG CCTGGCAAAG AACATCTGGT GGACACTCAG
    TGTTCCACTG CCTTGTCTGA GGAGCTAGAG AATGACAGGG AAGGCAGAGC TATGCATTCA
    TTGCATTCAC AGCTTCATGA TCTCTCTCTT CAGGAACCCA ACAATGACAA TTTGGAGCCT
    TCTCAAAATC AGCCACAGCA ACAGATGCCC TTGACACCCT TCTCGCCTCA TAATACCCCA
    GGCATTTTCT TGGCCCCTGG TGCAGGGCTT CTAGAAGGAG CTCCAGAAGG TATCCAGGAA
    GTCAGAAATA TGGGACCCAG AAATACTTCT GCTCACTCCA GACCCTCATA TCGTTCTGCT
    TCTTGGTCTT CTGATTCTGG TAGGCCCAAG AATATGGGCA CACATCCTTC AGTCCAAAAA
    GAAGAAGCCT TTGAAATAAT TGTTGAGTTT CCAGAAACCA ACTGCGATGT CAAAGACAGG
    CAGGGGAAAG AGCAGGGAGA AGAAATTAGT GAAAGAGGCG CAGGCCCTAC ATTTAAAGCT
    AGTCCCTCCT GGGTTGACCC AGAAGGAGAA ACAGCAGAAA GCACTGAAGA TGCACCCTTA
    GACTTTCACA GGGTCCTGCA CAATTCTCTG GGAAACATTT CCATGCTGCC ATGTAGCTCC
    TTCACCCCTA ATTGGCCTGT TCAAAATCCT GACTCCAGAA AAAGTGGTGG CCCAGTCGCA
    GAGCAGGGCA TCGACCCTGA TGCCTCCACA GTGGATGAGG AGGGGCAACT GCTCGACAGC
    ATGGATGTTC CCTGCACAAA TGGGCACGGC TCTCATAGAC TGTGCATTCT GAGACAGCCG
    CCTGGTCAGA GGGCGGAGAC CCCCAATTCC TCTGTAAGCG GTAACATCCT CTTCCCTGTC
    CTCAGCGAGG ACTGCACTAC CACAGAGGAA GGAAATCAGC CTGGAAACAT GCTAAACTGC
    AGCCAGAACT CCAGCTCATC CTCAGTGTGG TGGCTGAAAT CACCTGCATT TTCCAGTGGT
    TCTTCTGAGG GGGACAGCCC TTGGTCCTAT CTGAATTCCA GTGGGAGTTC TTGGGTTTCA
    TTGCCGGGAA AGATGAGGAA AGAGATCCTT GAGGCTCGCA CCTTGCAACC TGATGACTTT
    GAAAAGCTGT TGGCAGGAGT GAGGCATGAT TGGCTGTTTC AGAGACTAGA GAATACGGGG
    GTTTTTAAGC CCAGTCAACT CCACCGAGCA CATAGTGCTC TTTTGTTAAA ATATTCAAAA
    AAATCTGAAC TGTGGACGGC CCAGGAAACT ATTGTCTATT TGGGGGACTA CTTGACTGTG
    AAGAAAAAAG GCAGACAAAG AAATGCTTTT TGGGTTCATC ATCTTCATCA AGAAGAAATT
    CTGGGGAGGT ATGTTGGGAA AGACTATAAG GAGCAGAAGG GGCTCTGGCA CCACTTCACT
    GATGTGGAGC GACAGATGAC CGCACAGCAC TATGTGACAG AATTTAACAA GAGACTCTAT
    GAACAAAACA TTCCCACCCA GATATTCTAC ATCCCATCCA CAATACTACT GATTTTAGAG
    GACAAGACAA TAAAGGGATG TATCAGTGTG GAGCCTTACA TACTGGGAGA ATTTGTAAAA
    TTGTCAAATA ACACGAAAGT GGTGAAAACA GAATACAAAG CCACAGAATA TGGCTTGGCC
    TATGGCCATT TTTCTTATGA GTTTTCTAAT CATAGAGATG TTGTGGTCGA TTTACAAGGT
    TGGGTAACCG GTAATGGAAA AGGACTCATC TACCTCACAG ATCCCCAGAT TCACTCCGTT
    GATCAGAAAG TTTTCACTAC CAATTTTGGA AAGAGAGGAA TTTTTTACTT CTTTAATAAC
    CAGCATGTGG AATGTAATGA AATCTGCCAT CGTCTTTCTT TGACTAGACC TTCAATGGAG
    AAACCATGTA AGTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu39101
    Clone ID Related Accession (Same CDS sequence) XM_006714326.3
    Accession Version XM_006714326.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3678bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product alpha-protein kinase 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714326.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGAGCCT GCTGAAGGAG TTTGACCACC ATTTGCTGTC CGCTGCAGAA
    GCCTGCAAGC TGGCAGCTGC CTTCAGTGCC TATACGCCGC TCTTCGTGCT CACAGCTGTG
    AATATCCGTG GCACGTGTTT ATTGTCCTAC AGTAGTTCAA ATGACTGTCC TCCAGAATTG
    AAAAACTTAC ATCTGTGTGA AGCCAAAGAG GCCTTTGAGA TTGGCCTCCT CACCAAGAGA
    GATGATGAGC CTGTTACTGG AAAACAGGAG CTTCACAGCT TTGTCAAAGC TGCTTTCGGT
    CTCACCACAG TGCACAGAAG GCTCCATGGG GAGACAGGGA CGGTCCATGC AGCAAGTCAG
    CTCTGTAAGG AAGCAATGGG GAAGCTGTAC AATTTCAGCA CTTCCTCCAG AAGTCAGGAC
    AGAGAAGCTC TGTCTCAAGA AGTTATGTCT GTGATTGCCC AGGTGAAGGA ACATTTACAA
    GTTCAAAGCT TCTCAAATGT AGATGACAGA TCTTATGTTC CCGAGAGTTT CGAGTGCAGG
    TTGGATAAAC TTATCTTGCA TGGGCAAGGG GATTTCCAAA AAATCCTTGA CACCTATTCA
    CAGCACCATA CTTCGGTGTG TGAAGTATTT GAAAGTGATT GTGGAAACAA CAAAAATGAA
    CAGAAAGATG CAAAAACAGG AGTCTGCATC ACTGCTCTAA AAACAGAAAT AAAAAACATA
    GATACTGTGA GTACTACTCA AGAAAAGCCA CATTGTCAAA GAGACACAGG AATATCTTCC
    TCCCTAATGG GTAAGAATGT TCAGAGGGAA CTCAGAAGGG GAGGAAGGAG AAACTGGACC
    CATTCTGATG CATTTCGAGT CTCCTTGGAT CAAGATGTGG AGACTGAGAC TGAGCCATCG
    GACTACAGCA ATGGTGAGGG AGCTGTTTTC AACAAGTCTC TGAGTGGCAG CCAGACTTCC
    AGTGCTTGGA GCAACTTATC AGGGTTTAGT TCCTCTGCAA GCTGGGAGGA AGTGAATTAT
    CACGTTGACG ACAGGTCAGC CAGAAAAGAG CCTGGCAAAG AACATCTGGT GGACACTCAG
    TGTTCCACTG CCTTGTCTGA GGAGCTAGAG AATGACAGGG AAGGCAGAGC TATGCATTCA
    TTGCATTCAC AGCTTCATGA TCTCTCTCTT CAGGAACCCA ACAATGACAA TTTGGAGCCT
    TCTCAAAATC AGCCACAGCA ACAGATGCCC TTGACACCCT TCTCGCCTCA TAATACCCCA
    GGCATTTTCT TGGCCCCTGG TGCAGGGCTT CTAGAAGGAG CTCCAGAAGG TATCCAGGAA
    GTCAGAAATA TGGGACCCAG AAATACTTCT GCTCACTCCA GACCCTCATA TCGTTCTGCT
    TCTTGGTCTT CTGATTCTGG TAGGCCCAAG AATATGGGCA CACATCCTTC AGTCCAAAAA
    GAAGAAGCCT TTGAAATAAT TGTTGAGTTT CCAGAAACCA ACTGCGATGT CAAAGACAGG
    CAGGGGAAAG AGCAGGGAGA AGAAATTAGT GAAAGAGGCG CAGGCCCTAC ATTTAAAGCT
    AGTCCCTCCT GGGTTGACCC AGAAGGAGAA ACAGCAGAAA GCACTGAAGA TGCACCCTTA
    GACTTTCACA GGGTCCTGCA CAATTCTCTG GGAAACATTT CCATGCTGCC ATGTAGCTCC
    TTCACCCCTA ATTGGCCTGT TCAAAATCCT GACTCCAGAA AAAGTGGTGG CCCAGTCGCA
    GAGCAGGGCA TCGACCCTGA TGCCTCCACA GTGGATGAGG AGGGGCAACT GCTCGACAGC
    ATGGATGTTC CCTGCACAAA TGGGCACGGC TCTCATAGAC TGTGCATTCT GAGACAGCCG
    CCTGGTCAGA GGGCGGAGAC CCCCAATTCC TCTGTAAGCG GTAACATCCT CTTCCCTGTC
    CTCAGCGAGG ACTGCACTAC CACAGAGGAA GGAAATCAGC CTGGAAACAT GCTAAACTGC
    AGCCAGAACT CCAGCTCATC CTCAGTGTGG TGGCTGAAAT CACCTGCATT TTCCAGTGGT
    TCTTCTGAGG GGGACAGCCC TTGGTCCTAT CTGAATTCCA GTGGGAGTTC TTGGGTTTCA
    TTGCCGGGAA AGATGAGGAA AGAGATCCTT GAGGCTCGCA CCTTGCAACC TGATGACTTT
    GAAAAGCTGT TGGCAGGAGT GAGGCATGAT TGGCTGTTTC AGAGACTAGA GAATACGGGG
    GTTTTTAAGC CCAGTCAACT CCACCGAGCA CATAGTGCTC TTTTGTTAAA ATATTCAAAA
    AAATCTGAAC TGTGGACGGC CCAGGAAACT ATTGTCTATT TGGGGGACTA CTTGACTGTG
    AAGAAAAAAG GCAGACAAAG AAATGCTTTT TGGGTTCATC ATCTTCATCA AGAAGAAATT
    CTGGGGAGGT ATGTTGGGAA AGACTATAAG GAGCAGAAGG GGCTCTGGCA CCACTTCACT
    GATGTGGAGC GACAGATGAC CGCACAGCAC TATGTGACAG AATTTAACAA GAGACTCTAT
    GAACAAAACA TTCCCACCCA GATATTCTAC ATCCCATCCA CAATACTACT GATTTTAGAG
    GACAAGACAA TAAAGGGATG TATCAGTGTG GAGCCTTACA TACTGGGAGA ATTTGTAAAA
    TTGTCAAATA ACACGAAAGT GGTGAAAACA GAATACAAAG CCACAGAATA TGGCTTGGCC
    TATGGCCATT TTTCTTATGA GTTTTCTAAT CATAGAGATG TTGTGGTCGA TTTACAAGGT
    TGGGTAACCG GTAATGGAAA AGGACTCATC TACCTCACAG ATCCCCAGAT TCACTCCGTT
    GATCAGAAAG TTTTCACTAC CAATTTTGGA AAGAGAGGAA TTTTTTACTT CTTTAATAAC
    CAGCATGTGG AATGTAATGA AATCTGCCAT CGTCTTTCTT TGACTAGACC TTCAATGGAG
    AAACCATGTA AGTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006714389.1
    CDS280..3957
    Translation

    Target ORF information:

    RefSeq Version XM_006714326.3
    Organism Homo sapiens(human)
    Definition Homo sapiens alpha kinase 1 (ALPK1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006714326.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGAATAATC AAAAAGTGGT AGCTGTGCTA CTGCAAGAGT GCAAGCAAGT GCTGGATCAG 
    CTCTTGTTGG AAGCGCCAGA TGTGTCGGAA GAGGACAAGA GCGAGGACCA GCGCTGCAGA
    GCTTTACTCC CCAGCGAGTT AAGGACCCTG ATCCAGGAGG CAAAGGAAAT GAAGTGGCCC
    TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCGTGGGCC CAGAGGACAA AACAAACCTG
    AAGGATGTGA TTGGCGCCGG GTTGCAGCAG TTACTGGCGT CCCTGAGGGC CTCCATCCTC
    GCTCGGGACT GTGCGGCTGC GGCGGCTATT GTGTTCTTGG TGGACCGGTT CCTGTATGGG
    CTCGACGTCT CTGGAAAACT TCTGCAGGTC GCCAAAGGTC TCCACAAGTT GCAGCCAGCC
    ACGCCAATTG CCCCGCAGGT GGTTATTCGC CAAGCCCGAA TCTCCGTGAA CTCAGGTACC
    TGGCTGTACA GAAATGAAAG TGACAAGGTC CTGGTGCAGT CGGTCTGTAT ACAGATCAGA
    GGGCAGATTC TGCAAAAGCT GGGGATGTGG TACGAAGCAG CAGAGTTAAT ATGGGCCTCC
    ATTGTAGGAT ATTTGGCACT TCCTCAGCCG GATAAAAAGG GCCTCTCCAC GTCGCTAGGT
    ATACTGGCAG ACATCTTTGT TTCCATGAGC AAGAACGATT ATGAAAAGTT TAAAAACAAT
    CCACAAATTA ATTTGAGCCT GCTGAAGGAG TTTGACCACC ATTTGCTGTC CGCTGCAGAA
    GCCTGCAAGC TGGCAGCTGC CTTCAGTGCC TATACGCCGC TCTTCGTGCT CACAGCTGTG
    AATATCCGTG GCACGTGTTT ATTGTCCTAC AGTAGTTCAA ATGACTGTCC TCCAGAATTG
    AAAAACTTAC ATCTGTGTGA AGCCAAAGAG GCCTTTGAGA TTGGCCTCCT CACCAAGAGA
    GATGATGAGC CTGTTACTGG AAAACAGGAG CTTCACAGCT TTGTCAAAGC TGCTTTCGGT
    CTCACCACAG TGCACAGAAG GCTCCATGGG GAGACAGGGA CGGTCCATGC AGCAAGTCAG
    CTCTGTAAGG AAGCAATGGG GAAGCTGTAC AATTTCAGCA CTTCCTCCAG AAGTCAGGAC
    AGAGAAGCTC TGTCTCAAGA AGTTATGTCT GTGATTGCCC AGGTGAAGGA ACATTTACAA
    GTTCAAAGCT TCTCAAATGT AGATGACAGA TCTTATGTTC CCGAGAGTTT CGAGTGCAGG
    TTGGATAAAC TTATCTTGCA TGGGCAAGGG GATTTCCAAA AAATCCTTGA CACCTATTCA
    CAGCACCATA CTTCGGTGTG TGAAGTATTT GAAAGTGATT GTGGAAACAA CAAAAATGAA
    CAGAAAGATG CAAAAACAGG AGTCTGCATC ACTGCTCTAA AAACAGAAAT AAAAAACATA
    GATACTGTGA GTACTACTCA AGAAAAGCCA CATTGTCAAA GAGACACAGG AATATCTTCC
    TCCCTAATGG GTAAGAATGT TCAGAGGGAA CTCAGAAGGG GAGGAAGGAG AAACTGGACC
    CATTCTGATG CATTTCGAGT CTCCTTGGAT CAAGATGTGG AGACTGAGAC TGAGCCATCG
    GACTACAGCA ATGGTGAGGG AGCTGTTTTC AACAAGTCTC TGAGTGGCAG CCAGACTTCC
    AGTGCTTGGA GCAACTTATC AGGGTTTAGT TCCTCTGCAA GCTGGGAGGA AGTGAATTAT
    CACGTTGACG ACAGGTCAGC CAGAAAAGAG CCTGGCAAAG AACATCTGGT GGACACTCAG
    TGTTCCACTG CCTTGTCTGA GGAGCTAGAG AATGACAGGG AAGGCAGAGC TATGCATTCA
    TTGCATTCAC AGCTTCATGA TCTCTCTCTT CAGGAACCCA ACAATGACAA TTTGGAGCCT
    TCTCAAAATC AGCCACAGCA ACAGATGCCC TTGACACCCT TCTCGCCTCA TAATACCCCA
    GGCATTTTCT TGGCCCCTGG TGCAGGGCTT CTAGAAGGAG CTCCAGAAGG TATCCAGGAA
    GTCAGAAATA TGGGACCCAG AAATACTTCT GCTCACTCCA GACCCTCATA TCGTTCTGCT
    TCTTGGTCTT CTGATTCTGG TAGGCCCAAG AATATGGGCA CACATCCTTC AGTCCAAAAA
    GAAGAAGCCT TTGAAATAAT TGTTGAGTTT CCAGAAACCA ACTGCGATGT CAAAGACAGG
    CAGGGGAAAG AGCAGGGAGA AGAAATTAGT GAAAGAGGCG CAGGCCCTAC ATTTAAAGCT
    AGTCCCTCCT GGGTTGACCC AGAAGGAGAA ACAGCAGAAA GCACTGAAGA TGCACCCTTA
    GACTTTCACA GGGTCCTGCA CAATTCTCTG GGAAACATTT CCATGCTGCC ATGTAGCTCC
    TTCACCCCTA ATTGGCCTGT TCAAAATCCT GACTCCAGAA AAAGTGGTGG CCCAGTCGCA
    GAGCAGGGCA TCGACCCTGA TGCCTCCACA GTGGATGAGG AGGGGCAACT GCTCGACAGC
    ATGGATGTTC CCTGCACAAA TGGGCACGGC TCTCATAGAC TGTGCATTCT GAGACAGCCG
    CCTGGTCAGA GGGCGGAGAC CCCCAATTCC TCTGTAAGCG GTAACATCCT CTTCCCTGTC
    CTCAGCGAGG ACTGCACTAC CACAGAGGAA GGAAATCAGC CTGGAAACAT GCTAAACTGC
    AGCCAGAACT CCAGCTCATC CTCAGTGTGG TGGCTGAAAT CACCTGCATT TTCCAGTGGT
    TCTTCTGAGG GGGACAGCCC TTGGTCCTAT CTGAATTCCA GTGGGAGTTC TTGGGTTTCA
    TTGCCGGGAA AGATGAGGAA AGAGATCCTT GAGGCTCGCA CCTTGCAACC TGATGACTTT
    GAAAAGCTGT TGGCAGGAGT GAGGCATGAT TGGCTGTTTC AGAGACTAGA GAATACGGGG
    GTTTTTAAGC CCAGTCAACT CCACCGAGCA CATAGTGCTC TTTTGTTAAA ATATTCAAAA
    AAATCTGAAC TGTGGACGGC CCAGGAAACT ATTGTCTATT TGGGGGACTA CTTGACTGTG
    AAGAAAAAAG GCAGACAAAG AAATGCTTTT TGGGTTCATC ATCTTCATCA AGAAGAAATT
    CTGGGGAGGT ATGTTGGGAA AGACTATAAG GAGCAGAAGG GGCTCTGGCA CCACTTCACT
    GATGTGGAGC GACAGATGAC CGCACAGCAC TATGTGACAG AATTTAACAA GAGACTCTAT
    GAACAAAACA TTCCCACCCA GATATTCTAC ATCCCATCCA CAATACTACT GATTTTAGAG
    GACAAGACAA TAAAGGGATG TATCAGTGTG GAGCCTTACA TACTGGGAGA ATTTGTAAAA
    TTGTCAAATA ACACGAAAGT GGTGAAAACA GAATACAAAG CCACAGAATA TGGCTTGGCC
    TATGGCCATT TTTCTTATGA GTTTTCTAAT CATAGAGATG TTGTGGTCGA TTTACAAGGT
    TGGGTAACCG GTAATGGAAA AGGACTCATC TACCTCACAG ATCCCCAGAT TCACTCCGTT
    GATCAGAAAG TTTTCACTAC CAATTTTGGA AAGAGAGGAA TTTTTTACTT CTTTAATAAC
    CAGCATGTGG AATGTAATGA AATCTGCCAT CGTCTTTCTT TGACTAGACC TTCAATGGAG
    AAACCATGTA AGTCATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.