Alpk1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Alpk1
Entrez Gene ID	310879
Full Name	alpha-kinase 1
Synonyms	RGD1304613
General protein information	Preferred Names alpha-kinase 1 Names alpha-protein kinase 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q42

mRNA and Protein(s)

mRNA	Protein	Name
XM_017596888.1	XP_017452377.1	alpha-protein kinase 1 isoform X1
XM_017591343.1	XP_017446832.1	alpha-protein kinase 1 isoform X1
XM_017596891.1	XP_017452380.1	alpha-protein kinase 1 isoform X1
XM_227715.8	XP_227715.5	alpha-protein kinase 1 isoform X2
XM_001076258.5	XP_001076258.2	alpha-protein kinase 1 isoform X2
XM_017591342.1	XP_017446831.1	alpha-protein kinase 1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ALPK1	XP_005639336.1
Bos taurus (cattle)	ALPK1	XP_005207724.1
Mus musculus (house mouse)	Alpk1	NP_082084.1
	ALPK1	XP_001093043.1
Gallus gallus (chicken)	ALPK1	XP_004936006.1
Xenopus tropicalis (tropical clawed frog)	alpk1	NP_001090743.1
Homo sapiens (human)	ALPK1	NP_001095876.1
Rattus norvegicus (Norway rat)	Alpk1	XP_227715.5
Pan troglodytes (chimpanzee)	ALPK1	XP_001142586.1

Gene Ontology

The following Alpk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Alpk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa54944	XM_017596891.1 Latest version!	Rattus norvegicus alpha-kinase 1 (Alpk1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa54944	XM_017591343.1 Latest version!	Rattus norvegicus alpha-kinase 1 (Alpk1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa54944	XM_017596888.1 Latest version!	Rattus norvegicus alpha-kinase 1 (Alpk1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa54944	XM_017591342.1 Latest version!	Rattus norvegicus alpha-kinase 1 (Alpk1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa15413	XM_001076258.5 Latest version!	Rattus norvegicus alpha-kinase 1 (Alpk1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa15413	XM_227715.8 Latest version!	Rattus norvegicus alpha-kinase 1 (Alpk1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Alpk1 ( XM_017596888.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452377.1 Rattus norvegicus alpha-kinase 1 (Alpk1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa54944
Clone ID Related Accession (Same CDS sequence)	XM_017596888.1 , XM_017591343.1 , XM_017596891.1 , XM_017591342.1
Accession Version	XM_017596888.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alpha-protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000070.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAATC AAGACGCTGT GGCTTCCATA CTGCACGAGT GCAAGCAAGT GCTGGACCGT 
CTCTTGTTGG AAACGCCAGA TGTGTCTACA GAGGACAAGG GTGAGGACCA GCGGTGCAGA 
GCCTCACTCT CCGGCGAGTT AAGGACCCTG ATCCAGGAGG CGAAGGAAAT GAAGTGGCCC 
TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCATGAGCC CAGAGGACAA AACAAACCTG 
CAGGACGTGA TTGGCGCCGG GCTGCAGCAG TTACTGAAAA TAAATGTTGC CACTAAGATG 
AGACCCACTA TGACTGGTGA AGAAAGAGCA GGGACCACAT CTGGAAAGTG GCCTGCCCCT 
AAAACATGGG TTTTGGGGGA CTACTTCAAC TGCCTGAAGC AAATGGACTT CAAAGAGAAG 
GGGCAGAACA TGTGCTGGAG TGCTATGTCA GAGAACATCA TCTCTAAGGC GGCCTTGCGA 
GCCTCCATCC TTGTCCAGGA CTGCGCTGCC GCCGCCGCTA TTGTCTTCTT GATGGACCGG 
TTCCTGTACG GACTCGACGT CTCGGGAAAA CTCCTGCAGG TCGCCAAAGG CTTGCACAAG 
CTGCAGCCCG CGACGCCCAT CGCCCCGCAG GTGGTCATCC GCCAAGCCCG GGTCTCTGTC 
AACTCAGGGA AACTGTTGAA GGCAGAGTAC ATCCTGAGCA GCCTGATATG CAACAGCGGT 
GCAACTGGTA CCTGGCTCTA TAGGAATGAG AGTGACAAGG TCCTGGTACA GTCGGTGTGC 
ATACAGATCA GAGGGCAAAT TCTGCAGAAG CTAGGCATGT GGTATGAAGC GGCAGAGTTG 
ATATGGGCCT CCGTCATAGG ATATTTGTCA CTCCCTCAGC CAGATAAGAA GGGCATTTCC 
ACGTCATTGG GCATACTGGC GGACATCTTT GTGTCTATGA GCAAAACTGA TTATGAGAAG 
TTTAAGAAGA GCCCAAAGGT TAACTTGGCC TTGCTCAAGG AGTTCGACCA TCGTTTATTG 
TCAGCTGCGG AAGCCTGCAA ACTGGCAGCT GCCTTCAGTG CCTACACGCC CCTGTTCGTG 
CTCACAGCTG TGAACATCCG TGGAACATGT CTATTGTCAT ACAGTTGTTC TGCTGACTGC 
CCTCCAGGAA TGAAAAGTGC GTATCTGCGT GAAGCCAAAG AAGCCTTTGA GATTGGCCTC 
CTCACCAAGA AAGACGGCGA GCTGGTTAGT GGAAAGCAGG AGCTACACAG CTTCATCAAA 
GCTGCCTTTG GCCTCGCCAC GGTGCACTGC AGGCTCCATG GGGAGACAGA TGCAGTCCGT 
GCAGCAAAGC AGCAGTGCAG CGCAGCTATG GGCAAGCTCT ACACCTTCAG CACTTCCCTC 
TCGAGCCAGG ACAGAGAAGT CCTGTCTCGG GAAATCATGT CCCTCATCAG CCAGGTGAAG 
GGACATCTAC ACGTTCAAAG CTTCCCAAAT GCAGATGATA GATCTTATGT CCCAGAGAGT 
TTCACGTGTG GTTTAGATAG GCCCATCTTG CATGGGCACG TGGATTTCCA ACAGCTTCTC 
GAAACCTACT CCCAGCACCA TACTTCAGTG TGCGAGGTAT TTGAAAGCAC GTGTAGGAAC 
AGCAGAGGCC ACCAGAGAGA CGCAAAGGCA GAGGTCTGTA TCACCACTCT GAAGACGGAA 
ACAAGCACTA TAGATACTGT GGGGGTTACT CTAGAGAAAG TGTGCTCCCA AGACAGCGGG 
AGTACAGCTT ATTCCAAGAT GTCTGAGAAA GATTGGGAGA AGCTCCAAAG AGTAAGGAGG 
AGAAACTGGA CCCATTCCGA GGCGTTTCGA GTCTCCCTGG ATCAAGATAC GGGGACGGAG 
ACTGAGCTAC CAAACCACAG CAATGGCAGG GCAGATGTCT CCAGCAAGCC TCTGAGAGAC 
AGCTCTAGCT CGAGTTCTTG GAGCAGACTG TCAGGGCTCA GCTCTTCCGC AAGCTGGGAG 
GAAGTGAACG GTGCTGTTCA GGATACAGAA AGGAAAGAGT CTAGCCAAGA GGAACATCTT 
GTGGACACTC AATGCTCCAC AGCAGTGTCT GAGGAACCCG AGGGGGACAG ACGTATATGT 
AGAGAGACGT ATTTGCTGTT TTCCAAGCTC CACGGTGTCT CTCTTCAGAC GACCGAAGAT 
CACAATGCAG AGTCTTCACA AAGTCAGTTA CACACACCCG CCCTTCCTCT CAACTCGACG 
ACCACTTGTT TGGCCTCAGA TGCAGGGCTC TTAGAACCCC CTGAAGGATC CAACACTACT 
TCTCTTCAGT CCAGTCCTTC ATGTGGTTCT GATGCCTGGT CCTTGGCTAG TTTGGGCAGG 
TTCACAGCTG TGACCACATA TCCTTCAGTT CAGGAAGAAG AAACTTCTGG AATAATTGGT 
GATTTCCCAG AATCCAAGTA TGACTTCAAA GACTGGAGTG GAGAAAAGAA TGAGGGAAAA 
CTCACTGAAA TATGCACGGG GCCTGAATTA ACGTTTGCCT CCTCCTCGGT AGACCCAGAA 
GGAGACACGG CAGAAAGCAC AGATGATGGC CCATCACACT CTCTGGTAGT CCCAGGAGGT 
TTACAAGGCA GCCATTCAAT GTCTACGCAT CGGACTTCCT TTTCAGATTG GTCTGTTCAA 
AATGCTGACT CAGCAAAGAT TGGCTGCTCC GTCAAAGACG AAGCTGTGGA CCCTGATGCT 
TCTACGGTGG ATGAGGAGGG CCAAATGCTT GACAGCACAG AGGTCTACTC CATTGGCCAA 
GATGGCGCTC ACAGACCACG TGCTTTAAGG TCCGGTCAGA GTGATGAGAG TCCAAAGTCC 
TTTGTAAATG GCCACGGTCC CTCCCCTATC TTCGATGAGG ACTTCAGCAC CACAGAGGAA 
GGAGAGGAAC TTGGGAGCAT GCTGAAGAGT AGCCAGAACT CCAGCTCATG CTCGCCGTGG 
TGGCTAAAAT CACCCGCCTT TTCCCGCAGT TCGTCTGAGG GGGAAAGCTC CTGGTCTCTG 
CTGAACTCTA GCCGAAGTTC CTTTGGCTCA CTGACAGGAC AGACCAGCCA AGAGATCCTT 
GAGGCTCGCA CCCTGCAGCC TGATGACCTT GAAAAGCTTC TAGCAGGGGT GAGGCATGAT 
TGGCTGCTTC AGAGACTGGA GAATACAGGA GTTTTGAAGT CCAATCAACT CCAACAAGCA 
CACAGTGCCC TCCTGCTAAA ATATTCCAAA AAGTCTGAGT TGTGGACGGC CCAGGAAACG 
GTGGTATATC TGGGAGACTA CCTGAAAGTG AAGAAGAAGG GCAAGCAGAG AAATGCATTC 
TGGGTCCACT ATCTCCATCA AGAGGAAACC CTGGGGAGAT ACGTTGGGAA AGAATATAAA 
GAGCGGAAGG GGCTCCGGTA CCACCTCACA GATGTGGAGC GGCAGATGAC AGCGCAGCAC 
TACGTGACAG AGTTTAACAA GAGACTCTAC GAGCAAAAGA TCCCAACGCA GATATTCTAC 
ATCCCGTCCA CAATATTGCT GATTTTGGAA GGCAGGACTA TAAAGGGGTG CATCAGTGTG 
GAGCCTTACA TCCTGGGAGA ATTCGTCAAG TTATCAAATA ACACCAAAGT CGTCAAGACC 
GAGTACAAAG CTACAGAATA CGGCTTGGCC TACGGCCATT TTTCTTACGA GTTTTCTAAC 
CACAGACATG TTGTGGTTGA TTTACAAGGT TGGGTGACTG GTAATGGAAA AGGCCTCATC 
TATCTCACAG ATCCTCAGAT TCACTCTGTA GATCAGAGAG ACGTCACTAC AAACTTTGGA 
AAGCGGGGCA TATTTTACTT CTTTAATAAC CAACATGCAA GCTGTAATGA AATATGCCAT 
CGTCTTTCTC TGACGAGACC TTCACTAGAG CAAACAAGTA AGATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452377.1
CDS	804..4691
Translation	MNNQDAVASILHECKQVLDRLLLETPDVSTEDKGEDQRCRASLSGELRTLIQEAKEMKWPFVPEKWQYKQAMSPEDKTNLQDVIGAGLQQLLKINVATKMRPTMTGEERAGTTSGKWPAPKTWVLGDYFNCLKQMDFKEKGQNMCWSAMSENIISKAALRASILVQDCAAAAAIVFLMDRFLYGLDVSGKLLQVAKGLHKLQPATPIAPQVVIRQARVSVNSGKLLKAEYILSSLICNSGATGTWLYRNESDKVLVQSVCIQIRGQILQKLGMWYEAAELIWASVIGYLSLPQPDKKGISTSLGILADIFVSMSKTDYEKFKKSPKVNLALLKEFDHRLLSAAEACKLAAAFSAYTPLFVLTAVNIRGTCLLSYSCSADCPPGMKSAYLREAKEAFEIGLLTKKDGELVSGKQELHSFIKAAFGLATVHCRLHGETDAVRAAKQQCSAAMGKLYTFSTSLSSQDREVLSREIMSLISQVKGHLHVQSFPNADDRSYVPESFTCGLDRPILHGHVDFQQLLETYSQHHTSVCEVFESTCRNSRGHQRDAKAEVCITTLKTETSTIDTVGVTLEKVCSQDSGSTAYSKMSEKDWEKLQRVRRRNWTHSEAFRVSLDQDTGTETELPNHSNGRADVSSKPLRDSSSSSSWSRLSGLSSSASWEEVNGAVQDTERKESSQEEHLVDTQCSTAVSEEPEGDRRICRETYLLFSKLHGVSLQTTEDHNAESSQSQLHTPALPLNSTTTCLASDAGLLEPPEGSNTTSLQSSPSCGSDAWSLASLGRFTAVTTYPSVQEEETSGIIGDFPESKYDFKDWSGEKNEGKLTEICTGPELTFASSSVDPEGDTAESTDDGPSHSLVVPGGLQGSHSMSTHRTSFSDWSVQNADSAKIGCSVKDEAVDPDASTVDEEGQMLDSTEVYSIGQDGAHRPRALRSGQSDESPKSFVNGHGPSPIFDEDFSTTEEGEELGSMLKSSQNSSSCSPWWLKSPAFSRSSSEGESSWSLLNSSRSSFGSLTGQTSQEILEARTLQPDDLEKLLAGVRHDWLLQRLENTGVLKSNQLQQAHSALLLKYSKKSELWTAQETVVYLGDYLKVKKKGKQRNAFWVHYLHQEETLGRYVGKEYKERKGLRYHLTDVERQMTAQHYVTEFNKRLYEQKIPTQIFYIPSTILLILEGRTIKGCISVEPYILGEFVKLSNNTKVVKTEYKATEYGLAYGHFSYEFSNHRHVVVDLQGWVTGNGKGLIYLTDPQIHSVDQRDVTTNFGKRGIFYFFNNQHASCNEICHRLSLTRPSLEQTSKI

Target ORF information:

RefSeq Version	XM_017596888.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alpha-kinase 1 (Alpk1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596888.1

ORF Insert Sequence:

ATGAATAATC AAGACGCTGT GGCTTCCATA CTGCACGAGT GCAAGCAAGT GCTGGACCGT 
CTCTTGTTGG AAACGCCAGA TGTGTCTACA GAGGACAAGG GTGAGGACCA GCGGTGCAGA 
GCCTCACTCT CCGGCGAGTT AAGGACCCTG ATCCAGGAGG CGAAGGAAAT GAAGTGGCCC 
TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCATGAGCC CAGAGGACAA AACAAACCTG 
CAGGACGTGA TTGGCGCCGG GCTGCAGCAG TTACTGAAAA TAAATGTTGC CACTAAGATG 
AGACCCACTA TGACTGGTGA AGAAAGAGCA GGGACCACAT CTGGAAAGTG GCCTGCCCCT 
AAAACATGGG TTTTGGGGGA CTACTTCAAC TGCCTGAAGC AAATGGACTT CAAAGAGAAG 
GGGCAGAACA TGTGCTGGAG TGCTATGTCA GAGAACATCA TCTCTAAGGC GGCCTTGCGA 
GCCTCCATCC TTGTCCAGGA CTGCGCTGCC GCCGCCGCTA TTGTCTTCTT GATGGACCGG 
TTCCTGTACG GACTCGACGT CTCGGGAAAA CTCCTGCAGG TCGCCAAAGG CTTGCACAAG 
CTGCAGCCCG CGACGCCCAT CGCCCCGCAG GTGGTCATCC GCCAAGCCCG GGTCTCTGTC 
AACTCAGGGA AACTGTTGAA GGCAGAGTAC ATCCTGAGCA GCCTGATATG CAACAGCGGT 
GCAACTGGTA CCTGGCTCTA TAGGAATGAG AGTGACAAGG TCCTGGTACA GTCGGTGTGC 
ATACAGATCA GAGGGCAAAT TCTGCAGAAG CTAGGCATGT GGTATGAAGC GGCAGAGTTG 
ATATGGGCCT CCGTCATAGG ATATTTGTCA CTCCCTCAGC CAGATAAGAA GGGCATTTCC 
ACGTCATTGG GCATACTGGC GGACATCTTT GTGTCTATGA GCAAAACTGA TTATGAGAAG 
TTTAAGAAGA GCCCAAAGGT TAACTTGGCC TTGCTCAAGG AGTTCGACCA TCGTTTATTG 
TCAGCTGCGG AAGCCTGCAA ACTGGCAGCT GCCTTCAGTG CCTACACGCC CCTGTTCGTG 
CTCACAGCTG TGAACATCCG TGGAACATGT CTATTGTCAT ACAGTTGTTC TGCTGACTGC 
CCTCCAGGAA TGAAAAGTGC GTATCTGCGT GAAGCCAAAG AAGCCTTTGA GATTGGCCTC 
CTCACCAAGA AAGACGGCGA GCTGGTTAGT GGAAAGCAGG AGCTACACAG CTTCATCAAA 
GCTGCCTTTG GCCTCGCCAC GGTGCACTGC AGGCTCCATG GGGAGACAGA TGCAGTCCGT 
GCAGCAAAGC AGCAGTGCAG CGCAGCTATG GGCAAGCTCT ACACCTTCAG CACTTCCCTC 
TCGAGCCAGG ACAGAGAAGT CCTGTCTCGG GAAATCATGT CCCTCATCAG CCAGGTGAAG 
GGACATCTAC ACGTTCAAAG CTTCCCAAAT GCAGATGATA GATCTTATGT CCCAGAGAGT 
TTCACGTGTG GTTTAGATAG GCCCATCTTG CATGGGCACG TGGATTTCCA ACAGCTTCTC 
GAAACCTACT CCCAGCACCA TACTTCAGTG TGCGAGGTAT TTGAAAGCAC GTGTAGGAAC 
AGCAGAGGCC ACCAGAGAGA CGCAAAGGCA GAGGTCTGTA TCACCACTCT GAAGACGGAA 
ACAAGCACTA TAGATACTGT GGGGGTTACT CTAGAGAAAG TGTGCTCCCA AGACAGCGGG 
AGTACAGCTT ATTCCAAGAT GTCTGAGAAA GATTGGGAGA AGCTCCAAAG AGTAAGGAGG 
AGAAACTGGA CCCATTCCGA GGCGTTTCGA GTCTCCCTGG ATCAAGATAC GGGGACGGAG 
ACTGAGCTAC CAAACCACAG CAATGGCAGG GCAGATGTCT CCAGCAAGCC TCTGAGAGAC 
AGCTCTAGCT CGAGTTCTTG GAGCAGACTG TCAGGGCTCA GCTCTTCCGC AAGCTGGGAG 
GAAGTGAACG GTGCTGTTCA GGATACAGAA AGGAAAGAGT CTAGCCAAGA GGAACATCTT 
GTGGACACTC AATGCTCCAC AGCAGTGTCT GAGGAACCCG AGGGGGACAG ACGTATATGT 
AGAGAGACGT ATTTGCTGTT TTCCAAGCTC CACGGTGTCT CTCTTCAGAC GACCGAAGAT 
CACAATGCAG AGTCTTCACA AAGTCAGTTA CACACACCCG CCCTTCCTCT CAACTCGACG 
ACCACTTGTT TGGCCTCAGA TGCAGGGCTC TTAGAACCCC CTGAAGGATC CAACACTACT 
TCTCTTCAGT CCAGTCCTTC ATGTGGTTCT GATGCCTGGT CCTTGGCTAG TTTGGGCAGG 
TTCACAGCTG TGACCACATA TCCTTCAGTT CAGGAAGAAG AAACTTCTGG AATAATTGGT 
GATTTCCCAG AATCCAAGTA TGACTTCAAA GACTGGAGTG GAGAAAAGAA TGAGGGAAAA 
CTCACTGAAA TATGCACGGG GCCTGAATTA ACGTTTGCCT CCTCCTCGGT AGACCCAGAA 
GGAGACACGG CAGAAAGCAC AGATGATGGC CCATCACACT CTCTGGTAGT CCCAGGAGGT 
TTACAAGGCA GCCATTCAAT GTCTACGCAT CGGACTTCCT TTTCAGATTG GTCTGTTCAA 
AATGCTGACT CAGCAAAGAT TGGCTGCTCC GTCAAAGACG AAGCTGTGGA CCCTGATGCT 
TCTACGGTGG ATGAGGAGGG CCAAATGCTT GACAGCACAG AGGTCTACTC CATTGGCCAA 
GATGGCGCTC ACAGACCACG TGCTTTAAGG TCCGGTCAGA GTGATGAGAG TCCAAAGTCC 
TTTGTAAATG GCCACGGTCC CTCCCCTATC TTCGATGAGG ACTTCAGCAC CACAGAGGAA 
GGAGAGGAAC TTGGGAGCAT GCTGAAGAGT AGCCAGAACT CCAGCTCATG CTCGCCGTGG 
TGGCTAAAAT CACCCGCCTT TTCCCGCAGT TCGTCTGAGG GGGAAAGCTC CTGGTCTCTG 
CTGAACTCTA GCCGAAGTTC CTTTGGCTCA CTGACAGGAC AGACCAGCCA AGAGATCCTT 
GAGGCTCGCA CCCTGCAGCC TGATGACCTT GAAAAGCTTC TAGCAGGGGT GAGGCATGAT 
TGGCTGCTTC AGAGACTGGA GAATACAGGA GTTTTGAAGT CCAATCAACT CCAACAAGCA 
CACAGTGCCC TCCTGCTAAA ATATTCCAAA AAGTCTGAGT TGTGGACGGC CCAGGAAACG 
GTGGTATATC TGGGAGACTA CCTGAAAGTG AAGAAGAAGG GCAAGCAGAG AAATGCATTC 
TGGGTCCACT ATCTCCATCA AGAGGAAACC CTGGGGAGAT ACGTTGGGAA AGAATATAAA 
GAGCGGAAGG GGCTCCGGTA CCACCTCACA GATGTGGAGC GGCAGATGAC AGCGCAGCAC 
TACGTGACAG AGTTTAACAA GAGACTCTAC GAGCAAAAGA TCCCAACGCA GATATTCTAC 
ATCCCGTCCA CAATATTGCT GATTTTGGAA GGCAGGACTA TAAAGGGGTG CATCAGTGTG 
GAGCCTTACA TCCTGGGAGA ATTCGTCAAG TTATCAAATA ACACCAAAGT CGTCAAGACC 
GAGTACAAAG CTACAGAATA CGGCTTGGCC TACGGCCATT TTTCTTACGA GTTTTCTAAC 
CACAGACATG TTGTGGTTGA TTTACAAGGT TGGGTGACTG GTAATGGAAA AGGCCTCATC 
TATCTCACAG ATCCTCAGAT TCACTCTGTA GATCAGAGAG ACGTCACTAC AAACTTTGGA 
AAGCGGGGCA TATTTTACTT CTTTAATAAC CAACATGCAA GCTGTAATGA AATATGCCAT 
CGTCTTTCTC TGACGAGACC TTCACTAGAG CAAACAAGTA AGATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alpk1 ( XM_017591343.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446832.1 Rattus norvegicus alpha-kinase 1 (Alpk1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa54944
Clone ID Related Accession (Same CDS sequence)	XM_017596888.1 , XM_017591343.1 , XM_017596891.1 , XM_017591342.1
Accession Version	XM_017591343.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alpha-protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAATC AAGACGCTGT GGCTTCCATA CTGCACGAGT GCAAGCAAGT GCTGGACCGT 
CTCTTGTTGG AAACGCCAGA TGTGTCTACA GAGGACAAGG GTGAGGACCA GCGGTGCAGA 
GCCTCACTCT CCGGCGAGTT AAGGACCCTG ATCCAGGAGG CGAAGGAAAT GAAGTGGCCC 
TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCATGAGCC CAGAGGACAA AACAAACCTG 
CAGGACGTGA TTGGCGCCGG GCTGCAGCAG TTACTGAAAA TAAATGTTGC CACTAAGATG 
AGACCCACTA TGACTGGTGA AGAAAGAGCA GGGACCACAT CTGGAAAGTG GCCTGCCCCT 
AAAACATGGG TTTTGGGGGA CTACTTCAAC TGCCTGAAGC AAATGGACTT CAAAGAGAAG 
GGGCAGAACA TGTGCTGGAG TGCTATGTCA GAGAACATCA TCTCTAAGGC GGCCTTGCGA 
GCCTCCATCC TTGTCCAGGA CTGCGCTGCC GCCGCCGCTA TTGTCTTCTT GATGGACCGG 
TTCCTGTACG GACTCGACGT CTCGGGAAAA CTCCTGCAGG TCGCCAAAGG CTTGCACAAG 
CTGCAGCCCG CGACGCCCAT CGCCCCGCAG GTGGTCATCC GCCAAGCCCG GGTCTCTGTC 
AACTCAGGGA AACTGTTGAA GGCAGAGTAC ATCCTGAGCA GCCTGATATG CAACAGCGGT 
GCAACTGGTA CCTGGCTCTA TAGGAATGAG AGTGACAAGG TCCTGGTACA GTCGGTGTGC 
ATACAGATCA GAGGGCAAAT TCTGCAGAAG CTAGGCATGT GGTATGAAGC GGCAGAGTTG 
ATATGGGCCT CCGTCATAGG ATATTTGTCA CTCCCTCAGC CAGATAAGAA GGGCATTTCC 
ACGTCATTGG GCATACTGGC GGACATCTTT GTGTCTATGA GCAAAACTGA TTATGAGAAG 
TTTAAGAAGA GCCCAAAGGT TAACTTGGCC TTGCTCAAGG AGTTCGACCA TCGTTTATTG 
TCAGCTGCGG AAGCCTGCAA ACTGGCAGCT GCCTTCAGTG CCTACACGCC CCTGTTCGTG 
CTCACAGCTG TGAACATCCG TGGAACATGT CTATTGTCAT ACAGTTGTTC TGCTGACTGC 
CCTCCAGGAA TGAAAAGTGC GTATCTGCGT GAAGCCAAAG AAGCCTTTGA GATTGGCCTC 
CTCACCAAGA AAGACGGCGA GCTGGTTAGT GGAAAGCAGG AGCTACACAG CTTCATCAAA 
GCTGCCTTTG GCCTCGCCAC GGTGCACTGC AGGCTCCATG GGGAGACAGA TGCAGTCCGT 
GCAGCAAAGC AGCAGTGCAG CGCAGCTATG GGCAAGCTCT ACACCTTCAG CACTTCCCTC 
TCGAGCCAGG ACAGAGAAGT CCTGTCTCGG GAAATCATGT CCCTCATCAG CCAGGTGAAG 
GGACATCTAC ACGTTCAAAG CTTCCCAAAT GCAGATGATA GATCTTATGT CCCAGAGAGT 
TTCACGTGTG GTTTAGATAG GCCCATCTTG CATGGGCACG TGGATTTCCA ACAGCTTCTC 
GAAACCTACT CCCAGCACCA TACTTCAGTG TGCGAGGTAT TTGAAAGCAC GTGTAGGAAC 
AGCAGAGGCC ACCAGAGAGA CGCAAAGGCA GAGGTCTGTA TCACCACTCT GAAGACGGAA 
ACAAGCACTA TAGATACTGT GGGGGTTACT CTAGAGAAAG TGTGCTCCCA AGACAGCGGG 
AGTACAGCTT ATTCCAAGAT GTCTGAGAAA GATTGGGAGA AGCTCCAAAG AGTAAGGAGG 
AGAAACTGGA CCCATTCCGA GGCGTTTCGA GTCTCCCTGG ATCAAGATAC GGGGACGGAG 
ACTGAGCTAC CAAACCACAG CAATGGCAGG GCAGATGTCT CCAGCAAGCC TCTGAGAGAC 
AGCTCTAGCT CGAGTTCTTG GAGCAGACTG TCAGGGCTCA GCTCTTCCGC AAGCTGGGAG 
GAAGTGAACG GTGCTGTTCA GGATACAGAA AGGAAAGAGT CTAGCCAAGA GGAACATCTT 
GTGGACACTC AATGCTCCAC AGCAGTGTCT GAGGAACCCG AGGGGGACAG ACGTATATGT 
AGAGAGACGT ATTTGCTGTT TTCCAAGCTC CACGGTGTCT CTCTTCAGAC GACCGAAGAT 
CACAATGCAG AGTCTTCACA AAGTCAGTTA CACACACCCG CCCTTCCTCT CAACTCGACG 
ACCACTTGTT TGGCCTCAGA TGCAGGGCTC TTAGAACCCC CTGAAGGATC CAACACTACT 
TCTCTTCAGT CCAGTCCTTC ATGTGGTTCT GATGCCTGGT CCTTGGCTAG TTTGGGCAGG 
TTCACAGCTG TGACCACATA TCCTTCAGTT CAGGAAGAAG AAACTTCTGG AATAATTGGT 
GATTTCCCAG AATCCAAGTA TGACTTCAAA GACTGGAGTG GAGAAAAGAA TGAGGGAAAA 
CTCACTGAAA TATGCACGGG GCCTGAATTA ACGTTTGCCT CCTCCTCGGT AGACCCAGAA 
GGAGACACGG CAGAAAGCAC AGATGATGGC CCATCACACT CTCTGGTAGT CCCAGGAGGT 
TTACAAGGCA GCCATTCAAT GTCTACGCAT CGGACTTCCT TTTCAGATTG GTCTGTTCAA 
AATGCTGACT CAGCAAAGAT TGGCTGCTCC GTCAAAGACG AAGCTGTGGA CCCTGATGCT 
TCTACGGTGG ATGAGGAGGG CCAAATGCTT GACAGCACAG AGGTCTACTC CATTGGCCAA 
GATGGCGCTC ACAGACCACG TGCTTTAAGG TCCGGTCAGA GTGATGAGAG TCCAAAGTCC 
TTTGTAAATG GCCACGGTCC CTCCCCTATC TTCGATGAGG ACTTCAGCAC CACAGAGGAA 
GGAGAGGAAC TTGGGAGCAT GCTGAAGAGT AGCCAGAACT CCAGCTCATG CTCGCCGTGG 
TGGCTAAAAT CACCCGCCTT TTCCCGCAGT TCGTCTGAGG GGGAAAGCTC CTGGTCTCTG 
CTGAACTCTA GCCGAAGTTC CTTTGGCTCA CTGACAGGAC AGACCAGCCA AGAGATCCTT 
GAGGCTCGCA CCCTGCAGCC TGATGACCTT GAAAAGCTTC TAGCAGGGGT GAGGCATGAT 
TGGCTGCTTC AGAGACTGGA GAATACAGGA GTTTTGAAGT CCAATCAACT CCAACAAGCA 
CACAGTGCCC TCCTGCTAAA ATATTCCAAA AAGTCTGAGT TGTGGACGGC CCAGGAAACG 
GTGGTATATC TGGGAGACTA CCTGAAAGTG AAGAAGAAGG GCAAGCAGAG AAATGCATTC 
TGGGTCCACT ATCTCCATCA AGAGGAAACC CTGGGGAGAT ACGTTGGGAA AGAATATAAA 
GAGCGGAAGG GGCTCCGGTA CCACCTCACA GATGTGGAGC GGCAGATGAC AGCGCAGCAC 
TACGTGACAG AGTTTAACAA GAGACTCTAC GAGCAAAAGA TCCCAACGCA GATATTCTAC 
ATCCCGTCCA CAATATTGCT GATTTTGGAA GGCAGGACTA TAAAGGGGTG CATCAGTGTG 
GAGCCTTACA TCCTGGGAGA ATTCGTCAAG TTATCAAATA ACACCAAAGT CGTCAAGACC 
GAGTACAAAG CTACAGAATA CGGCTTGGCC TACGGCCATT TTTCTTACGA GTTTTCTAAC 
CACAGACATG TTGTGGTTGA TTTACAAGGT TGGGTGACTG GTAATGGAAA AGGCCTCATC 
TATCTCACAG ATCCTCAGAT TCACTCTGTA GATCAGAGAG ACGTCACTAC AAACTTTGGA 
AAGCGGGGCA TATTTTACTT CTTTAATAAC CAACATGCAA GCTGTAATGA AATATGCCAT 
CGTCTTTCTC TGACGAGACC TTCACTAGAG CAAACAAGTA AGATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446832.1
CDS	697..4584
Translation	MNNQDAVASILHECKQVLDRLLLETPDVSTEDKGEDQRCRASLSGELRTLIQEAKEMKWPFVPEKWQYKQAMSPEDKTNLQDVIGAGLQQLLKINVATKMRPTMTGEERAGTTSGKWPAPKTWVLGDYFNCLKQMDFKEKGQNMCWSAMSENIISKAALRASILVQDCAAAAAIVFLMDRFLYGLDVSGKLLQVAKGLHKLQPATPIAPQVVIRQARVSVNSGKLLKAEYILSSLICNSGATGTWLYRNESDKVLVQSVCIQIRGQILQKLGMWYEAAELIWASVIGYLSLPQPDKKGISTSLGILADIFVSMSKTDYEKFKKSPKVNLALLKEFDHRLLSAAEACKLAAAFSAYTPLFVLTAVNIRGTCLLSYSCSADCPPGMKSAYLREAKEAFEIGLLTKKDGELVSGKQELHSFIKAAFGLATVHCRLHGETDAVRAAKQQCSAAMGKLYTFSTSLSSQDREVLSREIMSLISQVKGHLHVQSFPNADDRSYVPESFTCGLDRPILHGHVDFQQLLETYSQHHTSVCEVFESTCRNSRGHQRDAKAEVCITTLKTETSTIDTVGVTLEKVCSQDSGSTAYSKMSEKDWEKLQRVRRRNWTHSEAFRVSLDQDTGTETELPNHSNGRADVSSKPLRDSSSSSSWSRLSGLSSSASWEEVNGAVQDTERKESSQEEHLVDTQCSTAVSEEPEGDRRICRETYLLFSKLHGVSLQTTEDHNAESSQSQLHTPALPLNSTTTCLASDAGLLEPPEGSNTTSLQSSPSCGSDAWSLASLGRFTAVTTYPSVQEEETSGIIGDFPESKYDFKDWSGEKNEGKLTEICTGPELTFASSSVDPEGDTAESTDDGPSHSLVVPGGLQGSHSMSTHRTSFSDWSVQNADSAKIGCSVKDEAVDPDASTVDEEGQMLDSTEVYSIGQDGAHRPRALRSGQSDESPKSFVNGHGPSPIFDEDFSTTEEGEELGSMLKSSQNSSSCSPWWLKSPAFSRSSSEGESSWSLLNSSRSSFGSLTGQTSQEILEARTLQPDDLEKLLAGVRHDWLLQRLENTGVLKSNQLQQAHSALLLKYSKKSELWTAQETVVYLGDYLKVKKKGKQRNAFWVHYLHQEETLGRYVGKEYKERKGLRYHLTDVERQMTAQHYVTEFNKRLYEQKIPTQIFYIPSTILLILEGRTIKGCISVEPYILGEFVKLSNNTKVVKTEYKATEYGLAYGHFSYEFSNHRHVVVDLQGWVTGNGKGLIYLTDPQIHSVDQRDVTTNFGKRGIFYFFNNQHASCNEICHRLSLTRPSLEQTSKI

Target ORF information:

RefSeq Version	XM_017591343.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alpha-kinase 1 (Alpk1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591343.1

ORF Insert Sequence:

ATGAATAATC AAGACGCTGT GGCTTCCATA CTGCACGAGT GCAAGCAAGT GCTGGACCGT 
CTCTTGTTGG AAACGCCAGA TGTGTCTACA GAGGACAAGG GTGAGGACCA GCGGTGCAGA 
GCCTCACTCT CCGGCGAGTT AAGGACCCTG ATCCAGGAGG CGAAGGAAAT GAAGTGGCCC 
TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCATGAGCC CAGAGGACAA AACAAACCTG 
CAGGACGTGA TTGGCGCCGG GCTGCAGCAG TTACTGAAAA TAAATGTTGC CACTAAGATG 
AGACCCACTA TGACTGGTGA AGAAAGAGCA GGGACCACAT CTGGAAAGTG GCCTGCCCCT 
AAAACATGGG TTTTGGGGGA CTACTTCAAC TGCCTGAAGC AAATGGACTT CAAAGAGAAG 
GGGCAGAACA TGTGCTGGAG TGCTATGTCA GAGAACATCA TCTCTAAGGC GGCCTTGCGA 
GCCTCCATCC TTGTCCAGGA CTGCGCTGCC GCCGCCGCTA TTGTCTTCTT GATGGACCGG 
TTCCTGTACG GACTCGACGT CTCGGGAAAA CTCCTGCAGG TCGCCAAAGG CTTGCACAAG 
CTGCAGCCCG CGACGCCCAT CGCCCCGCAG GTGGTCATCC GCCAAGCCCG GGTCTCTGTC 
AACTCAGGGA AACTGTTGAA GGCAGAGTAC ATCCTGAGCA GCCTGATATG CAACAGCGGT 
GCAACTGGTA CCTGGCTCTA TAGGAATGAG AGTGACAAGG TCCTGGTACA GTCGGTGTGC 
ATACAGATCA GAGGGCAAAT TCTGCAGAAG CTAGGCATGT GGTATGAAGC GGCAGAGTTG 
ATATGGGCCT CCGTCATAGG ATATTTGTCA CTCCCTCAGC CAGATAAGAA GGGCATTTCC 
ACGTCATTGG GCATACTGGC GGACATCTTT GTGTCTATGA GCAAAACTGA TTATGAGAAG 
TTTAAGAAGA GCCCAAAGGT TAACTTGGCC TTGCTCAAGG AGTTCGACCA TCGTTTATTG 
TCAGCTGCGG AAGCCTGCAA ACTGGCAGCT GCCTTCAGTG CCTACACGCC CCTGTTCGTG 
CTCACAGCTG TGAACATCCG TGGAACATGT CTATTGTCAT ACAGTTGTTC TGCTGACTGC 
CCTCCAGGAA TGAAAAGTGC GTATCTGCGT GAAGCCAAAG AAGCCTTTGA GATTGGCCTC 
CTCACCAAGA AAGACGGCGA GCTGGTTAGT GGAAAGCAGG AGCTACACAG CTTCATCAAA 
GCTGCCTTTG GCCTCGCCAC GGTGCACTGC AGGCTCCATG GGGAGACAGA TGCAGTCCGT 
GCAGCAAAGC AGCAGTGCAG CGCAGCTATG GGCAAGCTCT ACACCTTCAG CACTTCCCTC 
TCGAGCCAGG ACAGAGAAGT CCTGTCTCGG GAAATCATGT CCCTCATCAG CCAGGTGAAG 
GGACATCTAC ACGTTCAAAG CTTCCCAAAT GCAGATGATA GATCTTATGT CCCAGAGAGT 
TTCACGTGTG GTTTAGATAG GCCCATCTTG CATGGGCACG TGGATTTCCA ACAGCTTCTC 
GAAACCTACT CCCAGCACCA TACTTCAGTG TGCGAGGTAT TTGAAAGCAC GTGTAGGAAC 
AGCAGAGGCC ACCAGAGAGA CGCAAAGGCA GAGGTCTGTA TCACCACTCT GAAGACGGAA 
ACAAGCACTA TAGATACTGT GGGGGTTACT CTAGAGAAAG TGTGCTCCCA AGACAGCGGG 
AGTACAGCTT ATTCCAAGAT GTCTGAGAAA GATTGGGAGA AGCTCCAAAG AGTAAGGAGG 
AGAAACTGGA CCCATTCCGA GGCGTTTCGA GTCTCCCTGG ATCAAGATAC GGGGACGGAG 
ACTGAGCTAC CAAACCACAG CAATGGCAGG GCAGATGTCT CCAGCAAGCC TCTGAGAGAC 
AGCTCTAGCT CGAGTTCTTG GAGCAGACTG TCAGGGCTCA GCTCTTCCGC AAGCTGGGAG 
GAAGTGAACG GTGCTGTTCA GGATACAGAA AGGAAAGAGT CTAGCCAAGA GGAACATCTT 
GTGGACACTC AATGCTCCAC AGCAGTGTCT GAGGAACCCG AGGGGGACAG ACGTATATGT 
AGAGAGACGT ATTTGCTGTT TTCCAAGCTC CACGGTGTCT CTCTTCAGAC GACCGAAGAT 
CACAATGCAG AGTCTTCACA AAGTCAGTTA CACACACCCG CCCTTCCTCT CAACTCGACG 
ACCACTTGTT TGGCCTCAGA TGCAGGGCTC TTAGAACCCC CTGAAGGATC CAACACTACT 
TCTCTTCAGT CCAGTCCTTC ATGTGGTTCT GATGCCTGGT CCTTGGCTAG TTTGGGCAGG 
TTCACAGCTG TGACCACATA TCCTTCAGTT CAGGAAGAAG AAACTTCTGG AATAATTGGT 
GATTTCCCAG AATCCAAGTA TGACTTCAAA GACTGGAGTG GAGAAAAGAA TGAGGGAAAA 
CTCACTGAAA TATGCACGGG GCCTGAATTA ACGTTTGCCT CCTCCTCGGT AGACCCAGAA 
GGAGACACGG CAGAAAGCAC AGATGATGGC CCATCACACT CTCTGGTAGT CCCAGGAGGT 
TTACAAGGCA GCCATTCAAT GTCTACGCAT CGGACTTCCT TTTCAGATTG GTCTGTTCAA 
AATGCTGACT CAGCAAAGAT TGGCTGCTCC GTCAAAGACG AAGCTGTGGA CCCTGATGCT 
TCTACGGTGG ATGAGGAGGG CCAAATGCTT GACAGCACAG AGGTCTACTC CATTGGCCAA 
GATGGCGCTC ACAGACCACG TGCTTTAAGG TCCGGTCAGA GTGATGAGAG TCCAAAGTCC 
TTTGTAAATG GCCACGGTCC CTCCCCTATC TTCGATGAGG ACTTCAGCAC CACAGAGGAA 
GGAGAGGAAC TTGGGAGCAT GCTGAAGAGT AGCCAGAACT CCAGCTCATG CTCGCCGTGG 
TGGCTAAAAT CACCCGCCTT TTCCCGCAGT TCGTCTGAGG GGGAAAGCTC CTGGTCTCTG 
CTGAACTCTA GCCGAAGTTC CTTTGGCTCA CTGACAGGAC AGACCAGCCA AGAGATCCTT 
GAGGCTCGCA CCCTGCAGCC TGATGACCTT GAAAAGCTTC TAGCAGGGGT GAGGCATGAT 
TGGCTGCTTC AGAGACTGGA GAATACAGGA GTTTTGAAGT CCAATCAACT CCAACAAGCA 
CACAGTGCCC TCCTGCTAAA ATATTCCAAA AAGTCTGAGT TGTGGACGGC CCAGGAAACG 
GTGGTATATC TGGGAGACTA CCTGAAAGTG AAGAAGAAGG GCAAGCAGAG AAATGCATTC 
TGGGTCCACT ATCTCCATCA AGAGGAAACC CTGGGGAGAT ACGTTGGGAA AGAATATAAA 
GAGCGGAAGG GGCTCCGGTA CCACCTCACA GATGTGGAGC GGCAGATGAC AGCGCAGCAC 
TACGTGACAG AGTTTAACAA GAGACTCTAC GAGCAAAAGA TCCCAACGCA GATATTCTAC 
ATCCCGTCCA CAATATTGCT GATTTTGGAA GGCAGGACTA TAAAGGGGTG CATCAGTGTG 
GAGCCTTACA TCCTGGGAGA ATTCGTCAAG TTATCAAATA ACACCAAAGT CGTCAAGACC 
GAGTACAAAG CTACAGAATA CGGCTTGGCC TACGGCCATT TTTCTTACGA GTTTTCTAAC 
CACAGACATG TTGTGGTTGA TTTACAAGGT TGGGTGACTG GTAATGGAAA AGGCCTCATC 
TATCTCACAG ATCCTCAGAT TCACTCTGTA GATCAGAGAG ACGTCACTAC AAACTTTGGA 
AAGCGGGGCA TATTTTACTT CTTTAATAAC CAACATGCAA GCTGTAATGA AATATGCCAT 
CGTCTTTCTC TGACGAGACC TTCACTAGAG CAAACAAGTA AGATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alpk1 ( XM_017596891.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452380.1 Rattus norvegicus alpha-kinase 1 (Alpk1), transcript variant X2, mRNA.

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CloneID	ORa54944
Clone ID Related Accession (Same CDS sequence)	XM_017596888.1 , XM_017591343.1 , XM_017596891.1 , XM_017591342.1
Accession Version	XM_017596891.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alpha-protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000070.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAATC AAGACGCTGT GGCTTCCATA CTGCACGAGT GCAAGCAAGT GCTGGACCGT 
CTCTTGTTGG AAACGCCAGA TGTGTCTACA GAGGACAAGG GTGAGGACCA GCGGTGCAGA 
GCCTCACTCT CCGGCGAGTT AAGGACCCTG ATCCAGGAGG CGAAGGAAAT GAAGTGGCCC 
TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCATGAGCC CAGAGGACAA AACAAACCTG 
CAGGACGTGA TTGGCGCCGG GCTGCAGCAG TTACTGAAAA TAAATGTTGC CACTAAGATG 
AGACCCACTA TGACTGGTGA AGAAAGAGCA GGGACCACAT CTGGAAAGTG GCCTGCCCCT 
AAAACATGGG TTTTGGGGGA CTACTTCAAC TGCCTGAAGC AAATGGACTT CAAAGAGAAG 
GGGCAGAACA TGTGCTGGAG TGCTATGTCA GAGAACATCA TCTCTAAGGC GGCCTTGCGA 
GCCTCCATCC TTGTCCAGGA CTGCGCTGCC GCCGCCGCTA TTGTCTTCTT GATGGACCGG 
TTCCTGTACG GACTCGACGT CTCGGGAAAA CTCCTGCAGG TCGCCAAAGG CTTGCACAAG 
CTGCAGCCCG CGACGCCCAT CGCCCCGCAG GTGGTCATCC GCCAAGCCCG GGTCTCTGTC 
AACTCAGGGA AACTGTTGAA GGCAGAGTAC ATCCTGAGCA GCCTGATATG CAACAGCGGT 
GCAACTGGTA CCTGGCTCTA TAGGAATGAG AGTGACAAGG TCCTGGTACA GTCGGTGTGC 
ATACAGATCA GAGGGCAAAT TCTGCAGAAG CTAGGCATGT GGTATGAAGC GGCAGAGTTG 
ATATGGGCCT CCGTCATAGG ATATTTGTCA CTCCCTCAGC CAGATAAGAA GGGCATTTCC 
ACGTCATTGG GCATACTGGC GGACATCTTT GTGTCTATGA GCAAAACTGA TTATGAGAAG 
TTTAAGAAGA GCCCAAAGGT TAACTTGGCC TTGCTCAAGG AGTTCGACCA TCGTTTATTG 
TCAGCTGCGG AAGCCTGCAA ACTGGCAGCT GCCTTCAGTG CCTACACGCC CCTGTTCGTG 
CTCACAGCTG TGAACATCCG TGGAACATGT CTATTGTCAT ACAGTTGTTC TGCTGACTGC 
CCTCCAGGAA TGAAAAGTGC GTATCTGCGT GAAGCCAAAG AAGCCTTTGA GATTGGCCTC 
CTCACCAAGA AAGACGGCGA GCTGGTTAGT GGAAAGCAGG AGCTACACAG CTTCATCAAA 
GCTGCCTTTG GCCTCGCCAC GGTGCACTGC AGGCTCCATG GGGAGACAGA TGCAGTCCGT 
GCAGCAAAGC AGCAGTGCAG CGCAGCTATG GGCAAGCTCT ACACCTTCAG CACTTCCCTC 
TCGAGCCAGG ACAGAGAAGT CCTGTCTCGG GAAATCATGT CCCTCATCAG CCAGGTGAAG 
GGACATCTAC ACGTTCAAAG CTTCCCAAAT GCAGATGATA GATCTTATGT CCCAGAGAGT 
TTCACGTGTG GTTTAGATAG GCCCATCTTG CATGGGCACG TGGATTTCCA ACAGCTTCTC 
GAAACCTACT CCCAGCACCA TACTTCAGTG TGCGAGGTAT TTGAAAGCAC GTGTAGGAAC 
AGCAGAGGCC ACCAGAGAGA CGCAAAGGCA GAGGTCTGTA TCACCACTCT GAAGACGGAA 
ACAAGCACTA TAGATACTGT GGGGGTTACT CTAGAGAAAG TGTGCTCCCA AGACAGCGGG 
AGTACAGCTT ATTCCAAGAT GTCTGAGAAA GATTGGGAGA AGCTCCAAAG AGTAAGGAGG 
AGAAACTGGA CCCATTCCGA GGCGTTTCGA GTCTCCCTGG ATCAAGATAC GGGGACGGAG 
ACTGAGCTAC CAAACCACAG CAATGGCAGG GCAGATGTCT CCAGCAAGCC TCTGAGAGAC 
AGCTCTAGCT CGAGTTCTTG GAGCAGACTG TCAGGGCTCA GCTCTTCCGC AAGCTGGGAG 
GAAGTGAACG GTGCTGTTCA GGATACAGAA AGGAAAGAGT CTAGCCAAGA GGAACATCTT 
GTGGACACTC AATGCTCCAC AGCAGTGTCT GAGGAACCCG AGGGGGACAG ACGTATATGT 
AGAGAGACGT ATTTGCTGTT TTCCAAGCTC CACGGTGTCT CTCTTCAGAC GACCGAAGAT 
CACAATGCAG AGTCTTCACA AAGTCAGTTA CACACACCCG CCCTTCCTCT CAACTCGACG 
ACCACTTGTT TGGCCTCAGA TGCAGGGCTC TTAGAACCCC CTGAAGGATC CAACACTACT 
TCTCTTCAGT CCAGTCCTTC ATGTGGTTCT GATGCCTGGT CCTTGGCTAG TTTGGGCAGG 
TTCACAGCTG TGACCACATA TCCTTCAGTT CAGGAAGAAG AAACTTCTGG AATAATTGGT 
GATTTCCCAG AATCCAAGTA TGACTTCAAA GACTGGAGTG GAGAAAAGAA TGAGGGAAAA 
CTCACTGAAA TATGCACGGG GCCTGAATTA ACGTTTGCCT CCTCCTCGGT AGACCCAGAA 
GGAGACACGG CAGAAAGCAC AGATGATGGC CCATCACACT CTCTGGTAGT CCCAGGAGGT 
TTACAAGGCA GCCATTCAAT GTCTACGCAT CGGACTTCCT TTTCAGATTG GTCTGTTCAA 
AATGCTGACT CAGCAAAGAT TGGCTGCTCC GTCAAAGACG AAGCTGTGGA CCCTGATGCT 
TCTACGGTGG ATGAGGAGGG CCAAATGCTT GACAGCACAG AGGTCTACTC CATTGGCCAA 
GATGGCGCTC ACAGACCACG TGCTTTAAGG TCCGGTCAGA GTGATGAGAG TCCAAAGTCC 
TTTGTAAATG GCCACGGTCC CTCCCCTATC TTCGATGAGG ACTTCAGCAC CACAGAGGAA 
GGAGAGGAAC TTGGGAGCAT GCTGAAGAGT AGCCAGAACT CCAGCTCATG CTCGCCGTGG 
TGGCTAAAAT CACCCGCCTT TTCCCGCAGT TCGTCTGAGG GGGAAAGCTC CTGGTCTCTG 
CTGAACTCTA GCCGAAGTTC CTTTGGCTCA CTGACAGGAC AGACCAGCCA AGAGATCCTT 
GAGGCTCGCA CCCTGCAGCC TGATGACCTT GAAAAGCTTC TAGCAGGGGT GAGGCATGAT 
TGGCTGCTTC AGAGACTGGA GAATACAGGA GTTTTGAAGT CCAATCAACT CCAACAAGCA 
CACAGTGCCC TCCTGCTAAA ATATTCCAAA AAGTCTGAGT TGTGGACGGC CCAGGAAACG 
GTGGTATATC TGGGAGACTA CCTGAAAGTG AAGAAGAAGG GCAAGCAGAG AAATGCATTC 
TGGGTCCACT ATCTCCATCA AGAGGAAACC CTGGGGAGAT ACGTTGGGAA AGAATATAAA 
GAGCGGAAGG GGCTCCGGTA CCACCTCACA GATGTGGAGC GGCAGATGAC AGCGCAGCAC 
TACGTGACAG AGTTTAACAA GAGACTCTAC GAGCAAAAGA TCCCAACGCA GATATTCTAC 
ATCCCGTCCA CAATATTGCT GATTTTGGAA GGCAGGACTA TAAAGGGGTG CATCAGTGTG 
GAGCCTTACA TCCTGGGAGA ATTCGTCAAG TTATCAAATA ACACCAAAGT CGTCAAGACC 
GAGTACAAAG CTACAGAATA CGGCTTGGCC TACGGCCATT TTTCTTACGA GTTTTCTAAC 
CACAGACATG TTGTGGTTGA TTTACAAGGT TGGGTGACTG GTAATGGAAA AGGCCTCATC 
TATCTCACAG ATCCTCAGAT TCACTCTGTA GATCAGAGAG ACGTCACTAC AAACTTTGGA 
AAGCGGGGCA TATTTTACTT CTTTAATAAC CAACATGCAA GCTGTAATGA AATATGCCAT 
CGTCTTTCTC TGACGAGACC TTCACTAGAG CAAACAAGTA AGATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452380.1
CDS	697..4584
Translation	MNNQDAVASILHECKQVLDRLLLETPDVSTEDKGEDQRCRASLSGELRTLIQEAKEMKWPFVPEKWQYKQAMSPEDKTNLQDVIGAGLQQLLKINVATKMRPTMTGEERAGTTSGKWPAPKTWVLGDYFNCLKQMDFKEKGQNMCWSAMSENIISKAALRASILVQDCAAAAAIVFLMDRFLYGLDVSGKLLQVAKGLHKLQPATPIAPQVVIRQARVSVNSGKLLKAEYILSSLICNSGATGTWLYRNESDKVLVQSVCIQIRGQILQKLGMWYEAAELIWASVIGYLSLPQPDKKGISTSLGILADIFVSMSKTDYEKFKKSPKVNLALLKEFDHRLLSAAEACKLAAAFSAYTPLFVLTAVNIRGTCLLSYSCSADCPPGMKSAYLREAKEAFEIGLLTKKDGELVSGKQELHSFIKAAFGLATVHCRLHGETDAVRAAKQQCSAAMGKLYTFSTSLSSQDREVLSREIMSLISQVKGHLHVQSFPNADDRSYVPESFTCGLDRPILHGHVDFQQLLETYSQHHTSVCEVFESTCRNSRGHQRDAKAEVCITTLKTETSTIDTVGVTLEKVCSQDSGSTAYSKMSEKDWEKLQRVRRRNWTHSEAFRVSLDQDTGTETELPNHSNGRADVSSKPLRDSSSSSSWSRLSGLSSSASWEEVNGAVQDTERKESSQEEHLVDTQCSTAVSEEPEGDRRICRETYLLFSKLHGVSLQTTEDHNAESSQSQLHTPALPLNSTTTCLASDAGLLEPPEGSNTTSLQSSPSCGSDAWSLASLGRFTAVTTYPSVQEEETSGIIGDFPESKYDFKDWSGEKNEGKLTEICTGPELTFASSSVDPEGDTAESTDDGPSHSLVVPGGLQGSHSMSTHRTSFSDWSVQNADSAKIGCSVKDEAVDPDASTVDEEGQMLDSTEVYSIGQDGAHRPRALRSGQSDESPKSFVNGHGPSPIFDEDFSTTEEGEELGSMLKSSQNSSSCSPWWLKSPAFSRSSSEGESSWSLLNSSRSSFGSLTGQTSQEILEARTLQPDDLEKLLAGVRHDWLLQRLENTGVLKSNQLQQAHSALLLKYSKKSELWTAQETVVYLGDYLKVKKKGKQRNAFWVHYLHQEETLGRYVGKEYKERKGLRYHLTDVERQMTAQHYVTEFNKRLYEQKIPTQIFYIPSTILLILEGRTIKGCISVEPYILGEFVKLSNNTKVVKTEYKATEYGLAYGHFSYEFSNHRHVVVDLQGWVTGNGKGLIYLTDPQIHSVDQRDVTTNFGKRGIFYFFNNQHASCNEICHRLSLTRPSLEQTSKI

Target ORF information:

RefSeq Version	XM_017596891.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alpha-kinase 1 (Alpk1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596891.1

ORF Insert Sequence:

ATGAATAATC AAGACGCTGT GGCTTCCATA CTGCACGAGT GCAAGCAAGT GCTGGACCGT 
CTCTTGTTGG AAACGCCAGA TGTGTCTACA GAGGACAAGG GTGAGGACCA GCGGTGCAGA 
GCCTCACTCT CCGGCGAGTT AAGGACCCTG ATCCAGGAGG CGAAGGAAAT GAAGTGGCCC 
TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCATGAGCC CAGAGGACAA AACAAACCTG 
CAGGACGTGA TTGGCGCCGG GCTGCAGCAG TTACTGAAAA TAAATGTTGC CACTAAGATG 
AGACCCACTA TGACTGGTGA AGAAAGAGCA GGGACCACAT CTGGAAAGTG GCCTGCCCCT 
AAAACATGGG TTTTGGGGGA CTACTTCAAC TGCCTGAAGC AAATGGACTT CAAAGAGAAG 
GGGCAGAACA TGTGCTGGAG TGCTATGTCA GAGAACATCA TCTCTAAGGC GGCCTTGCGA 
GCCTCCATCC TTGTCCAGGA CTGCGCTGCC GCCGCCGCTA TTGTCTTCTT GATGGACCGG 
TTCCTGTACG GACTCGACGT CTCGGGAAAA CTCCTGCAGG TCGCCAAAGG CTTGCACAAG 
CTGCAGCCCG CGACGCCCAT CGCCCCGCAG GTGGTCATCC GCCAAGCCCG GGTCTCTGTC 
AACTCAGGGA AACTGTTGAA GGCAGAGTAC ATCCTGAGCA GCCTGATATG CAACAGCGGT 
GCAACTGGTA CCTGGCTCTA TAGGAATGAG AGTGACAAGG TCCTGGTACA GTCGGTGTGC 
ATACAGATCA GAGGGCAAAT TCTGCAGAAG CTAGGCATGT GGTATGAAGC GGCAGAGTTG 
ATATGGGCCT CCGTCATAGG ATATTTGTCA CTCCCTCAGC CAGATAAGAA GGGCATTTCC 
ACGTCATTGG GCATACTGGC GGACATCTTT GTGTCTATGA GCAAAACTGA TTATGAGAAG 
TTTAAGAAGA GCCCAAAGGT TAACTTGGCC TTGCTCAAGG AGTTCGACCA TCGTTTATTG 
TCAGCTGCGG AAGCCTGCAA ACTGGCAGCT GCCTTCAGTG CCTACACGCC CCTGTTCGTG 
CTCACAGCTG TGAACATCCG TGGAACATGT CTATTGTCAT ACAGTTGTTC TGCTGACTGC 
CCTCCAGGAA TGAAAAGTGC GTATCTGCGT GAAGCCAAAG AAGCCTTTGA GATTGGCCTC 
CTCACCAAGA AAGACGGCGA GCTGGTTAGT GGAAAGCAGG AGCTACACAG CTTCATCAAA 
GCTGCCTTTG GCCTCGCCAC GGTGCACTGC AGGCTCCATG GGGAGACAGA TGCAGTCCGT 
GCAGCAAAGC AGCAGTGCAG CGCAGCTATG GGCAAGCTCT ACACCTTCAG CACTTCCCTC 
TCGAGCCAGG ACAGAGAAGT CCTGTCTCGG GAAATCATGT CCCTCATCAG CCAGGTGAAG 
GGACATCTAC ACGTTCAAAG CTTCCCAAAT GCAGATGATA GATCTTATGT CCCAGAGAGT 
TTCACGTGTG GTTTAGATAG GCCCATCTTG CATGGGCACG TGGATTTCCA ACAGCTTCTC 
GAAACCTACT CCCAGCACCA TACTTCAGTG TGCGAGGTAT TTGAAAGCAC GTGTAGGAAC 
AGCAGAGGCC ACCAGAGAGA CGCAAAGGCA GAGGTCTGTA TCACCACTCT GAAGACGGAA 
ACAAGCACTA TAGATACTGT GGGGGTTACT CTAGAGAAAG TGTGCTCCCA AGACAGCGGG 
AGTACAGCTT ATTCCAAGAT GTCTGAGAAA GATTGGGAGA AGCTCCAAAG AGTAAGGAGG 
AGAAACTGGA CCCATTCCGA GGCGTTTCGA GTCTCCCTGG ATCAAGATAC GGGGACGGAG 
ACTGAGCTAC CAAACCACAG CAATGGCAGG GCAGATGTCT CCAGCAAGCC TCTGAGAGAC 
AGCTCTAGCT CGAGTTCTTG GAGCAGACTG TCAGGGCTCA GCTCTTCCGC AAGCTGGGAG 
GAAGTGAACG GTGCTGTTCA GGATACAGAA AGGAAAGAGT CTAGCCAAGA GGAACATCTT 
GTGGACACTC AATGCTCCAC AGCAGTGTCT GAGGAACCCG AGGGGGACAG ACGTATATGT 
AGAGAGACGT ATTTGCTGTT TTCCAAGCTC CACGGTGTCT CTCTTCAGAC GACCGAAGAT 
CACAATGCAG AGTCTTCACA AAGTCAGTTA CACACACCCG CCCTTCCTCT CAACTCGACG 
ACCACTTGTT TGGCCTCAGA TGCAGGGCTC TTAGAACCCC CTGAAGGATC CAACACTACT 
TCTCTTCAGT CCAGTCCTTC ATGTGGTTCT GATGCCTGGT CCTTGGCTAG TTTGGGCAGG 
TTCACAGCTG TGACCACATA TCCTTCAGTT CAGGAAGAAG AAACTTCTGG AATAATTGGT 
GATTTCCCAG AATCCAAGTA TGACTTCAAA GACTGGAGTG GAGAAAAGAA TGAGGGAAAA 
CTCACTGAAA TATGCACGGG GCCTGAATTA ACGTTTGCCT CCTCCTCGGT AGACCCAGAA 
GGAGACACGG CAGAAAGCAC AGATGATGGC CCATCACACT CTCTGGTAGT CCCAGGAGGT 
TTACAAGGCA GCCATTCAAT GTCTACGCAT CGGACTTCCT TTTCAGATTG GTCTGTTCAA 
AATGCTGACT CAGCAAAGAT TGGCTGCTCC GTCAAAGACG AAGCTGTGGA CCCTGATGCT 
TCTACGGTGG ATGAGGAGGG CCAAATGCTT GACAGCACAG AGGTCTACTC CATTGGCCAA 
GATGGCGCTC ACAGACCACG TGCTTTAAGG TCCGGTCAGA GTGATGAGAG TCCAAAGTCC 
TTTGTAAATG GCCACGGTCC CTCCCCTATC TTCGATGAGG ACTTCAGCAC CACAGAGGAA 
GGAGAGGAAC TTGGGAGCAT GCTGAAGAGT AGCCAGAACT CCAGCTCATG CTCGCCGTGG 
TGGCTAAAAT CACCCGCCTT TTCCCGCAGT TCGTCTGAGG GGGAAAGCTC CTGGTCTCTG 
CTGAACTCTA GCCGAAGTTC CTTTGGCTCA CTGACAGGAC AGACCAGCCA AGAGATCCTT 
GAGGCTCGCA CCCTGCAGCC TGATGACCTT GAAAAGCTTC TAGCAGGGGT GAGGCATGAT 
TGGCTGCTTC AGAGACTGGA GAATACAGGA GTTTTGAAGT CCAATCAACT CCAACAAGCA 
CACAGTGCCC TCCTGCTAAA ATATTCCAAA AAGTCTGAGT TGTGGACGGC CCAGGAAACG 
GTGGTATATC TGGGAGACTA CCTGAAAGTG AAGAAGAAGG GCAAGCAGAG AAATGCATTC 
TGGGTCCACT ATCTCCATCA AGAGGAAACC CTGGGGAGAT ACGTTGGGAA AGAATATAAA 
GAGCGGAAGG GGCTCCGGTA CCACCTCACA GATGTGGAGC GGCAGATGAC AGCGCAGCAC 
TACGTGACAG AGTTTAACAA GAGACTCTAC GAGCAAAAGA TCCCAACGCA GATATTCTAC 
ATCCCGTCCA CAATATTGCT GATTTTGGAA GGCAGGACTA TAAAGGGGTG CATCAGTGTG 
GAGCCTTACA TCCTGGGAGA ATTCGTCAAG TTATCAAATA ACACCAAAGT CGTCAAGACC 
GAGTACAAAG CTACAGAATA CGGCTTGGCC TACGGCCATT TTTCTTACGA GTTTTCTAAC 
CACAGACATG TTGTGGTTGA TTTACAAGGT TGGGTGACTG GTAATGGAAA AGGCCTCATC 
TATCTCACAG ATCCTCAGAT TCACTCTGTA GATCAGAGAG ACGTCACTAC AAACTTTGGA 
AAGCGGGGCA TATTTTACTT CTTTAATAAC CAACATGCAA GCTGTAATGA AATATGCCAT 
CGTCTTTCTC TGACGAGACC TTCACTAGAG CAAACAAGTA AGATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alpk1 ( XM_017591342.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446831.1 Rattus norvegicus alpha-kinase 1 (Alpk1), transcript variant X1, mRNA.

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CloneID	ORa54944
Clone ID Related Accession (Same CDS sequence)	XM_017596888.1 , XM_017591343.1 , XM_017596891.1 , XM_017591342.1
Accession Version	XM_017591342.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alpha-protein kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAATC AAGACGCTGT GGCTTCCATA CTGCACGAGT GCAAGCAAGT GCTGGACCGT 
CTCTTGTTGG AAACGCCAGA TGTGTCTACA GAGGACAAGG GTGAGGACCA GCGGTGCAGA 
GCCTCACTCT CCGGCGAGTT AAGGACCCTG ATCCAGGAGG CGAAGGAAAT GAAGTGGCCC 
TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCATGAGCC CAGAGGACAA AACAAACCTG 
CAGGACGTGA TTGGCGCCGG GCTGCAGCAG TTACTGAAAA TAAATGTTGC CACTAAGATG 
AGACCCACTA TGACTGGTGA AGAAAGAGCA GGGACCACAT CTGGAAAGTG GCCTGCCCCT 
AAAACATGGG TTTTGGGGGA CTACTTCAAC TGCCTGAAGC AAATGGACTT CAAAGAGAAG 
GGGCAGAACA TGTGCTGGAG TGCTATGTCA GAGAACATCA TCTCTAAGGC GGCCTTGCGA 
GCCTCCATCC TTGTCCAGGA CTGCGCTGCC GCCGCCGCTA TTGTCTTCTT GATGGACCGG 
TTCCTGTACG GACTCGACGT CTCGGGAAAA CTCCTGCAGG TCGCCAAAGG CTTGCACAAG 
CTGCAGCCCG CGACGCCCAT CGCCCCGCAG GTGGTCATCC GCCAAGCCCG GGTCTCTGTC 
AACTCAGGGA AACTGTTGAA GGCAGAGTAC ATCCTGAGCA GCCTGATATG CAACAGCGGT 
GCAACTGGTA CCTGGCTCTA TAGGAATGAG AGTGACAAGG TCCTGGTACA GTCGGTGTGC 
ATACAGATCA GAGGGCAAAT TCTGCAGAAG CTAGGCATGT GGTATGAAGC GGCAGAGTTG 
ATATGGGCCT CCGTCATAGG ATATTTGTCA CTCCCTCAGC CAGATAAGAA GGGCATTTCC 
ACGTCATTGG GCATACTGGC GGACATCTTT GTGTCTATGA GCAAAACTGA TTATGAGAAG 
TTTAAGAAGA GCCCAAAGGT TAACTTGGCC TTGCTCAAGG AGTTCGACCA TCGTTTATTG 
TCAGCTGCGG AAGCCTGCAA ACTGGCAGCT GCCTTCAGTG CCTACACGCC CCTGTTCGTG 
CTCACAGCTG TGAACATCCG TGGAACATGT CTATTGTCAT ACAGTTGTTC TGCTGACTGC 
CCTCCAGGAA TGAAAAGTGC GTATCTGCGT GAAGCCAAAG AAGCCTTTGA GATTGGCCTC 
CTCACCAAGA AAGACGGCGA GCTGGTTAGT GGAAAGCAGG AGCTACACAG CTTCATCAAA 
GCTGCCTTTG GCCTCGCCAC GGTGCACTGC AGGCTCCATG GGGAGACAGA TGCAGTCCGT 
GCAGCAAAGC AGCAGTGCAG CGCAGCTATG GGCAAGCTCT ACACCTTCAG CACTTCCCTC 
TCGAGCCAGG ACAGAGAAGT CCTGTCTCGG GAAATCATGT CCCTCATCAG CCAGGTGAAG 
GGACATCTAC ACGTTCAAAG CTTCCCAAAT GCAGATGATA GATCTTATGT CCCAGAGAGT 
TTCACGTGTG GTTTAGATAG GCCCATCTTG CATGGGCACG TGGATTTCCA ACAGCTTCTC 
GAAACCTACT CCCAGCACCA TACTTCAGTG TGCGAGGTAT TTGAAAGCAC GTGTAGGAAC 
AGCAGAGGCC ACCAGAGAGA CGCAAAGGCA GAGGTCTGTA TCACCACTCT GAAGACGGAA 
ACAAGCACTA TAGATACTGT GGGGGTTACT CTAGAGAAAG TGTGCTCCCA AGACAGCGGG 
AGTACAGCTT ATTCCAAGAT GTCTGAGAAA GATTGGGAGA AGCTCCAAAG AGTAAGGAGG 
AGAAACTGGA CCCATTCCGA GGCGTTTCGA GTCTCCCTGG ATCAAGATAC GGGGACGGAG 
ACTGAGCTAC CAAACCACAG CAATGGCAGG GCAGATGTCT CCAGCAAGCC TCTGAGAGAC 
AGCTCTAGCT CGAGTTCTTG GAGCAGACTG TCAGGGCTCA GCTCTTCCGC AAGCTGGGAG 
GAAGTGAACG GTGCTGTTCA GGATACAGAA AGGAAAGAGT CTAGCCAAGA GGAACATCTT 
GTGGACACTC AATGCTCCAC AGCAGTGTCT GAGGAACCCG AGGGGGACAG ACGTATATGT 
AGAGAGACGT ATTTGCTGTT TTCCAAGCTC CACGGTGTCT CTCTTCAGAC GACCGAAGAT 
CACAATGCAG AGTCTTCACA AAGTCAGTTA CACACACCCG CCCTTCCTCT CAACTCGACG 
ACCACTTGTT TGGCCTCAGA TGCAGGGCTC TTAGAACCCC CTGAAGGATC CAACACTACT 
TCTCTTCAGT CCAGTCCTTC ATGTGGTTCT GATGCCTGGT CCTTGGCTAG TTTGGGCAGG 
TTCACAGCTG TGACCACATA TCCTTCAGTT CAGGAAGAAG AAACTTCTGG AATAATTGGT 
GATTTCCCAG AATCCAAGTA TGACTTCAAA GACTGGAGTG GAGAAAAGAA TGAGGGAAAA 
CTCACTGAAA TATGCACGGG GCCTGAATTA ACGTTTGCCT CCTCCTCGGT AGACCCAGAA 
GGAGACACGG CAGAAAGCAC AGATGATGGC CCATCACACT CTCTGGTAGT CCCAGGAGGT 
TTACAAGGCA GCCATTCAAT GTCTACGCAT CGGACTTCCT TTTCAGATTG GTCTGTTCAA 
AATGCTGACT CAGCAAAGAT TGGCTGCTCC GTCAAAGACG AAGCTGTGGA CCCTGATGCT 
TCTACGGTGG ATGAGGAGGG CCAAATGCTT GACAGCACAG AGGTCTACTC CATTGGCCAA 
GATGGCGCTC ACAGACCACG TGCTTTAAGG TCCGGTCAGA GTGATGAGAG TCCAAAGTCC 
TTTGTAAATG GCCACGGTCC CTCCCCTATC TTCGATGAGG ACTTCAGCAC CACAGAGGAA 
GGAGAGGAAC TTGGGAGCAT GCTGAAGAGT AGCCAGAACT CCAGCTCATG CTCGCCGTGG 
TGGCTAAAAT CACCCGCCTT TTCCCGCAGT TCGTCTGAGG GGGAAAGCTC CTGGTCTCTG 
CTGAACTCTA GCCGAAGTTC CTTTGGCTCA CTGACAGGAC AGACCAGCCA AGAGATCCTT 
GAGGCTCGCA CCCTGCAGCC TGATGACCTT GAAAAGCTTC TAGCAGGGGT GAGGCATGAT 
TGGCTGCTTC AGAGACTGGA GAATACAGGA GTTTTGAAGT CCAATCAACT CCAACAAGCA 
CACAGTGCCC TCCTGCTAAA ATATTCCAAA AAGTCTGAGT TGTGGACGGC CCAGGAAACG 
GTGGTATATC TGGGAGACTA CCTGAAAGTG AAGAAGAAGG GCAAGCAGAG AAATGCATTC 
TGGGTCCACT ATCTCCATCA AGAGGAAACC CTGGGGAGAT ACGTTGGGAA AGAATATAAA 
GAGCGGAAGG GGCTCCGGTA CCACCTCACA GATGTGGAGC GGCAGATGAC AGCGCAGCAC 
TACGTGACAG AGTTTAACAA GAGACTCTAC GAGCAAAAGA TCCCAACGCA GATATTCTAC 
ATCCCGTCCA CAATATTGCT GATTTTGGAA GGCAGGACTA TAAAGGGGTG CATCAGTGTG 
GAGCCTTACA TCCTGGGAGA ATTCGTCAAG TTATCAAATA ACACCAAAGT CGTCAAGACC 
GAGTACAAAG CTACAGAATA CGGCTTGGCC TACGGCCATT TTTCTTACGA GTTTTCTAAC 
CACAGACATG TTGTGGTTGA TTTACAAGGT TGGGTGACTG GTAATGGAAA AGGCCTCATC 
TATCTCACAG ATCCTCAGAT TCACTCTGTA GATCAGAGAG ACGTCACTAC AAACTTTGGA 
AAGCGGGGCA TATTTTACTT CTTTAATAAC CAACATGCAA GCTGTAATGA AATATGCCAT 
CGTCTTTCTC TGACGAGACC TTCACTAGAG CAAACAAGTA AGATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446831.1
CDS	804..4691
Translation	MNNQDAVASILHECKQVLDRLLLETPDVSTEDKGEDQRCRASLSGELRTLIQEAKEMKWPFVPEKWQYKQAMSPEDKTNLQDVIGAGLQQLLKINVATKMRPTMTGEERAGTTSGKWPAPKTWVLGDYFNCLKQMDFKEKGQNMCWSAMSENIISKAALRASILVQDCAAAAAIVFLMDRFLYGLDVSGKLLQVAKGLHKLQPATPIAPQVVIRQARVSVNSGKLLKAEYILSSLICNSGATGTWLYRNESDKVLVQSVCIQIRGQILQKLGMWYEAAELIWASVIGYLSLPQPDKKGISTSLGILADIFVSMSKTDYEKFKKSPKVNLALLKEFDHRLLSAAEACKLAAAFSAYTPLFVLTAVNIRGTCLLSYSCSADCPPGMKSAYLREAKEAFEIGLLTKKDGELVSGKQELHSFIKAAFGLATVHCRLHGETDAVRAAKQQCSAAMGKLYTFSTSLSSQDREVLSREIMSLISQVKGHLHVQSFPNADDRSYVPESFTCGLDRPILHGHVDFQQLLETYSQHHTSVCEVFESTCRNSRGHQRDAKAEVCITTLKTETSTIDTVGVTLEKVCSQDSGSTAYSKMSEKDWEKLQRVRRRNWTHSEAFRVSLDQDTGTETELPNHSNGRADVSSKPLRDSSSSSSWSRLSGLSSSASWEEVNGAVQDTERKESSQEEHLVDTQCSTAVSEEPEGDRRICRETYLLFSKLHGVSLQTTEDHNAESSQSQLHTPALPLNSTTTCLASDAGLLEPPEGSNTTSLQSSPSCGSDAWSLASLGRFTAVTTYPSVQEEETSGIIGDFPESKYDFKDWSGEKNEGKLTEICTGPELTFASSSVDPEGDTAESTDDGPSHSLVVPGGLQGSHSMSTHRTSFSDWSVQNADSAKIGCSVKDEAVDPDASTVDEEGQMLDSTEVYSIGQDGAHRPRALRSGQSDESPKSFVNGHGPSPIFDEDFSTTEEGEELGSMLKSSQNSSSCSPWWLKSPAFSRSSSEGESSWSLLNSSRSSFGSLTGQTSQEILEARTLQPDDLEKLLAGVRHDWLLQRLENTGVLKSNQLQQAHSALLLKYSKKSELWTAQETVVYLGDYLKVKKKGKQRNAFWVHYLHQEETLGRYVGKEYKERKGLRYHLTDVERQMTAQHYVTEFNKRLYEQKIPTQIFYIPSTILLILEGRTIKGCISVEPYILGEFVKLSNNTKVVKTEYKATEYGLAYGHFSYEFSNHRHVVVDLQGWVTGNGKGLIYLTDPQIHSVDQRDVTTNFGKRGIFYFFNNQHASCNEICHRLSLTRPSLEQTSKI

Target ORF information:

RefSeq Version	XM_017591342.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alpha-kinase 1 (Alpk1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591342.1

ORF Insert Sequence:

ATGAATAATC AAGACGCTGT GGCTTCCATA CTGCACGAGT GCAAGCAAGT GCTGGACCGT 
CTCTTGTTGG AAACGCCAGA TGTGTCTACA GAGGACAAGG GTGAGGACCA GCGGTGCAGA 
GCCTCACTCT CCGGCGAGTT AAGGACCCTG ATCCAGGAGG CGAAGGAAAT GAAGTGGCCC 
TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCATGAGCC CAGAGGACAA AACAAACCTG 
CAGGACGTGA TTGGCGCCGG GCTGCAGCAG TTACTGAAAA TAAATGTTGC CACTAAGATG 
AGACCCACTA TGACTGGTGA AGAAAGAGCA GGGACCACAT CTGGAAAGTG GCCTGCCCCT 
AAAACATGGG TTTTGGGGGA CTACTTCAAC TGCCTGAAGC AAATGGACTT CAAAGAGAAG 
GGGCAGAACA TGTGCTGGAG TGCTATGTCA GAGAACATCA TCTCTAAGGC GGCCTTGCGA 
GCCTCCATCC TTGTCCAGGA CTGCGCTGCC GCCGCCGCTA TTGTCTTCTT GATGGACCGG 
TTCCTGTACG GACTCGACGT CTCGGGAAAA CTCCTGCAGG TCGCCAAAGG CTTGCACAAG 
CTGCAGCCCG CGACGCCCAT CGCCCCGCAG GTGGTCATCC GCCAAGCCCG GGTCTCTGTC 
AACTCAGGGA AACTGTTGAA GGCAGAGTAC ATCCTGAGCA GCCTGATATG CAACAGCGGT 
GCAACTGGTA CCTGGCTCTA TAGGAATGAG AGTGACAAGG TCCTGGTACA GTCGGTGTGC 
ATACAGATCA GAGGGCAAAT TCTGCAGAAG CTAGGCATGT GGTATGAAGC GGCAGAGTTG 
ATATGGGCCT CCGTCATAGG ATATTTGTCA CTCCCTCAGC CAGATAAGAA GGGCATTTCC 
ACGTCATTGG GCATACTGGC GGACATCTTT GTGTCTATGA GCAAAACTGA TTATGAGAAG 
TTTAAGAAGA GCCCAAAGGT TAACTTGGCC TTGCTCAAGG AGTTCGACCA TCGTTTATTG 
TCAGCTGCGG AAGCCTGCAA ACTGGCAGCT GCCTTCAGTG CCTACACGCC CCTGTTCGTG 
CTCACAGCTG TGAACATCCG TGGAACATGT CTATTGTCAT ACAGTTGTTC TGCTGACTGC 
CCTCCAGGAA TGAAAAGTGC GTATCTGCGT GAAGCCAAAG AAGCCTTTGA GATTGGCCTC 
CTCACCAAGA AAGACGGCGA GCTGGTTAGT GGAAAGCAGG AGCTACACAG CTTCATCAAA 
GCTGCCTTTG GCCTCGCCAC GGTGCACTGC AGGCTCCATG GGGAGACAGA TGCAGTCCGT 
GCAGCAAAGC AGCAGTGCAG CGCAGCTATG GGCAAGCTCT ACACCTTCAG CACTTCCCTC 
TCGAGCCAGG ACAGAGAAGT CCTGTCTCGG GAAATCATGT CCCTCATCAG CCAGGTGAAG 
GGACATCTAC ACGTTCAAAG CTTCCCAAAT GCAGATGATA GATCTTATGT CCCAGAGAGT 
TTCACGTGTG GTTTAGATAG GCCCATCTTG CATGGGCACG TGGATTTCCA ACAGCTTCTC 
GAAACCTACT CCCAGCACCA TACTTCAGTG TGCGAGGTAT TTGAAAGCAC GTGTAGGAAC 
AGCAGAGGCC ACCAGAGAGA CGCAAAGGCA GAGGTCTGTA TCACCACTCT GAAGACGGAA 
ACAAGCACTA TAGATACTGT GGGGGTTACT CTAGAGAAAG TGTGCTCCCA AGACAGCGGG 
AGTACAGCTT ATTCCAAGAT GTCTGAGAAA GATTGGGAGA AGCTCCAAAG AGTAAGGAGG 
AGAAACTGGA CCCATTCCGA GGCGTTTCGA GTCTCCCTGG ATCAAGATAC GGGGACGGAG 
ACTGAGCTAC CAAACCACAG CAATGGCAGG GCAGATGTCT CCAGCAAGCC TCTGAGAGAC 
AGCTCTAGCT CGAGTTCTTG GAGCAGACTG TCAGGGCTCA GCTCTTCCGC AAGCTGGGAG 
GAAGTGAACG GTGCTGTTCA GGATACAGAA AGGAAAGAGT CTAGCCAAGA GGAACATCTT 
GTGGACACTC AATGCTCCAC AGCAGTGTCT GAGGAACCCG AGGGGGACAG ACGTATATGT 
AGAGAGACGT ATTTGCTGTT TTCCAAGCTC CACGGTGTCT CTCTTCAGAC GACCGAAGAT 
CACAATGCAG AGTCTTCACA AAGTCAGTTA CACACACCCG CCCTTCCTCT CAACTCGACG 
ACCACTTGTT TGGCCTCAGA TGCAGGGCTC TTAGAACCCC CTGAAGGATC CAACACTACT 
TCTCTTCAGT CCAGTCCTTC ATGTGGTTCT GATGCCTGGT CCTTGGCTAG TTTGGGCAGG 
TTCACAGCTG TGACCACATA TCCTTCAGTT CAGGAAGAAG AAACTTCTGG AATAATTGGT 
GATTTCCCAG AATCCAAGTA TGACTTCAAA GACTGGAGTG GAGAAAAGAA TGAGGGAAAA 
CTCACTGAAA TATGCACGGG GCCTGAATTA ACGTTTGCCT CCTCCTCGGT AGACCCAGAA 
GGAGACACGG CAGAAAGCAC AGATGATGGC CCATCACACT CTCTGGTAGT CCCAGGAGGT 
TTACAAGGCA GCCATTCAAT GTCTACGCAT CGGACTTCCT TTTCAGATTG GTCTGTTCAA 
AATGCTGACT CAGCAAAGAT TGGCTGCTCC GTCAAAGACG AAGCTGTGGA CCCTGATGCT 
TCTACGGTGG ATGAGGAGGG CCAAATGCTT GACAGCACAG AGGTCTACTC CATTGGCCAA 
GATGGCGCTC ACAGACCACG TGCTTTAAGG TCCGGTCAGA GTGATGAGAG TCCAAAGTCC 
TTTGTAAATG GCCACGGTCC CTCCCCTATC TTCGATGAGG ACTTCAGCAC CACAGAGGAA 
GGAGAGGAAC TTGGGAGCAT GCTGAAGAGT AGCCAGAACT CCAGCTCATG CTCGCCGTGG 
TGGCTAAAAT CACCCGCCTT TTCCCGCAGT TCGTCTGAGG GGGAAAGCTC CTGGTCTCTG 
CTGAACTCTA GCCGAAGTTC CTTTGGCTCA CTGACAGGAC AGACCAGCCA AGAGATCCTT 
GAGGCTCGCA CCCTGCAGCC TGATGACCTT GAAAAGCTTC TAGCAGGGGT GAGGCATGAT 
TGGCTGCTTC AGAGACTGGA GAATACAGGA GTTTTGAAGT CCAATCAACT CCAACAAGCA 
CACAGTGCCC TCCTGCTAAA ATATTCCAAA AAGTCTGAGT TGTGGACGGC CCAGGAAACG 
GTGGTATATC TGGGAGACTA CCTGAAAGTG AAGAAGAAGG GCAAGCAGAG AAATGCATTC 
TGGGTCCACT ATCTCCATCA AGAGGAAACC CTGGGGAGAT ACGTTGGGAA AGAATATAAA 
GAGCGGAAGG GGCTCCGGTA CCACCTCACA GATGTGGAGC GGCAGATGAC AGCGCAGCAC 
TACGTGACAG AGTTTAACAA GAGACTCTAC GAGCAAAAGA TCCCAACGCA GATATTCTAC 
ATCCCGTCCA CAATATTGCT GATTTTGGAA GGCAGGACTA TAAAGGGGTG CATCAGTGTG 
GAGCCTTACA TCCTGGGAGA ATTCGTCAAG TTATCAAATA ACACCAAAGT CGTCAAGACC 
GAGTACAAAG CTACAGAATA CGGCTTGGCC TACGGCCATT TTTCTTACGA GTTTTCTAAC 
CACAGACATG TTGTGGTTGA TTTACAAGGT TGGGTGACTG GTAATGGAAA AGGCCTCATC 
TATCTCACAG ATCCTCAGAT TCACTCTGTA GATCAGAGAG ACGTCACTAC AAACTTTGGA 
AAGCGGGGCA TATTTTACTT CTTTAATAAC CAACATGCAA GCTGTAATGA AATATGCCAT 
CGTCTTTCTC TGACGAGACC TTCACTAGAG CAAACAAGTA AGATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alpk1 ( XM_227715.8 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_227715.5 Rattus norvegicus alpha-kinase 1 (Alpk1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa15413
Clone ID Related Accession (Same CDS sequence)	XM_227715.8 , XM_001076258.5
Accession Version	XM_227715.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alpha-protein kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_227715.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAATC AAGACGCTGT GGCTTCCATA CTGCACGAGT GCAAGCAAGT GCTGGACCGT 
CTCTTGTTGG AAACGCCAGA TGTGTCTACA GAGGACAAGG GTGAGGACCA GCGGTGCAGA 
GCCTCACTCT CCGGCGAGTT AAGGACCCTG ATCCAGGAGG CGAAGGAAAT GAAGTGGCCC 
TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCATGAGCC CAGAGGACAA AACAAACCTG 
CAGGACGTGA TTGGCGCCGG GCTGCAGCAG TTACTGGCGG CCTTGCGAGC CTCCATCCTT 
GTCCAGGACT GCGCTGCCGC CGCCGCTATT GTCTTCTTGA TGGACCGGTT CCTGTACGGA 
CTCGACGTCT CGGGAAAACT CCTGCAGGTC GCCAAAGGCT TGCACAAGCT GCAGCCCGCG 
ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAAGCCCGGG TCTCTGTCAA CTCAGGGAAA 
CTGTTGAAGG CAGAGTACAT CCTGAGCAGC CTGATATGCA ACAGCGGTGC AACTGGTACC 
TGGCTCTATA GGAATGAGAG TGACAAGGTC CTGGTACAGT CGGTGTGCAT ACAGATCAGA 
GGGCAAATTC TGCAGAAGCT AGGCATGTGG TATGAAGCGG CAGAGTTGAT ATGGGCCTCC 
GTCATAGGAT ATTTGTCACT CCCTCAGCCA GATAAGAAGG GCATTTCCAC GTCATTGGGC 
ATACTGGCGG ACATCTTTGT GTCTATGAGC AAAACTGATT ATGAGAAGTT TAAGAAGAGC 
CCAAAGGTTA ACTTGGCCTT GCTCAAGGAG TTCGACCATC GTTTATTGTC AGCTGCGGAA 
GCCTGCAAAC TGGCAGCTGC CTTCAGTGCC TACACGCCCC TGTTCGTGCT CACAGCTGTG 
AACATCCGTG GAACATGTCT ATTGTCATAC AGTTGTTCTG CTGACTGCCC TCCAGGAATG 
AAAAGTGCGT ATCTGCGTGA AGCCAAAGAA GCCTTTGAGA TTGGCCTCCT CACCAAGAAA 
GACGGCGAGC TGGTTAGTGG AAAGCAGGAG CTACACAGCT TCATCAAAGC TGCCTTTGGC 
CTCGCCACGG TGCACTGCAG GCTCCATGGG GAGACAGATG CAGTCCGTGC AGCAAAGCAG 
CAGTGCAGCG CAGCTATGGG CAAGCTCTAC ACCTTCAGCA CTTCCCTCTC GAGCCAGGAC 
AGAGAAGTCC TGTCTCGGGA AATCATGTCC CTCATCAGCC AGGTGAAGGG ACATCTACAC 
GTTCAAAGCT TCCCAAATGC AGATGATAGA TCTTATGTCC CAGAGAGTTT CACGTGTGGT 
TTAGATAGGC CCATCTTGCA TGGGCACGTG GATTTCCAAC AGCTTCTCGA AACCTACTCC 
CAGCACCATA CTTCAGTGTG CGAGGTATTT GAAAGCACGT GTAGGAACAG CAGAGGCCAC 
CAGAGAGACG CAAAGGCAGA GGTCTGTATC ACCACTCTGA AGACGGAAAC AAGCACTATA 
GATACTGTGG GGGTTACTCT AGAGAAAGTG TGCTCCCAAG ACAGCGGGAG TACAGCTTAT 
TCCAAGATGT CTGAGAAAGA TTGGGAGAAG CTCCAAAGAG TAAGGAGGAG AAACTGGACC 
CATTCCGAGG CGTTTCGAGT CTCCCTGGAT CAAGATACGG GGACGGAGAC TGAGCTACCA 
AACCACAGCA ATGGCAGGGC AGATGTCTCC AGCAAGCCTC TGAGAGACAG CTCTAGCTCG 
AGTTCTTGGA GCAGACTGTC AGGGCTCAGC TCTTCCGCAA GCTGGGAGGA AGTGAACGGT 
GCTGTTCAGG ATACAGAAAG GAAAGAGTCT AGCCAAGAGG AACATCTTGT GGACACTCAA 
TGCTCCACAG CAGTGTCTGA GGAACCCGAG GGGGACAGAC GTATATGTAG AGAGACGTAT 
TTGCTGTTTT CCAAGCTCCA CGGTGTCTCT CTTCAGACGA CCGAAGATCA CAATGCAGAG 
TCTTCACAAA GTCAGTTACA CACACCCGCC CTTCCTCTCA ACTCGACGAC CACTTGTTTG 
GCCTCAGATG CAGGGCTCTT AGAACCCCCT GAAGGATCCA ACACTACTTC TCTTCAGTCC 
AGTCCTTCAT GTGGTTCTGA TGCCTGGTCC TTGGCTAGTT TGGGCAGGTT CACAGCTGTG 
ACCACATATC CTTCAGTTCA GGAAGAAGAA ACTTCTGGAA TAATTGGTGA TTTCCCAGAA 
TCCAAGTATG ACTTCAAAGA CTGGAGTGGA GAAAAGAATG AGGGAAAACT CACTGAAATA 
TGCACGGGGC CTGAATTAAC GTTTGCCTCC TCCTCGGTAG ACCCAGAAGG AGACACGGCA 
GAAAGCACAG ATGATGGCCC ATCACACTCT CTGGTAGTCC CAGGAGGTTT ACAAGGCAGC 
CATTCAATGT CTACGCATCG GACTTCCTTT TCAGATTGGT CTGTTCAAAA TGCTGACTCA 
GCAAAGATTG GCTGCTCCGT CAAAGACGAA GCTGTGGACC CTGATGCTTC TACGGTGGAT 
GAGGAGGGCC AAATGCTTGA CAGCACAGAG GTCTACTCCA TTGGCCAAGA TGGCGCTCAC 
AGACCACGTG CTTTAAGGTC CGGTCAGAGT GATGAGAGTC CAAAGTCCTT TGTAAATGGC 
CACGGTCCCT CCCCTATCTT CGATGAGGAC TTCAGCACCA CAGAGGAAGG AGAGGAACTT 
GGGAGCATGC TGAAGAGTAG CCAGAACTCC AGCTCATGCT CGCCGTGGTG GCTAAAATCA 
CCCGCCTTTT CCCGCAGTTC GTCTGAGGGG GAAAGCTCCT GGTCTCTGCT GAACTCTAGC 
CGAAGTTCCT TTGGCTCACT GACAGGACAG ACCAGCCAAG AGATCCTTGA GGCTCGCACC 
CTGCAGCCTG ATGACCTTGA AAAGCTTCTA GCAGGGGTGA GGCATGATTG GCTGCTTCAG 
AGACTGGAGA ATACAGGAGT TTTGAAGTCC AATCAACTCC AACAAGCACA CAGTGCCCTC 
CTGCTAAAAT ATTCCAAAAA GTCTGAGTTG TGGACGGCCC AGGAAACGGT GGTATATCTG 
GGAGACTACC TGAAAGTGAA GAAGAAGGGC AAGCAGAGAA ATGCATTCTG GGTCCACTAT 
CTCCATCAAG AGGAAACCCT GGGGAGATAC GTTGGGAAAG AATATAAAGA GCGGAAGGGG 
CTCCGGTACC ACCTCACAGA TGTGGAGCGG CAGATGACAG CGCAGCACTA CGTGACAGAG 
TTTAACAAGA GACTCTACGA GCAAAAGATC CCAACGCAGA TATTCTACAT CCCGTCCACA 
ATATTGCTGA TTTTGGAAGG CAGGACTATA AAGGGGTGCA TCAGTGTGGA GCCTTACATC 
CTGGGAGAAT TCGTCAAGTT ATCAAATAAC ACCAAAGTCG TCAAGACCGA GTACAAAGCT 
ACAGAATACG GCTTGGCCTA CGGCCATTTT TCTTACGAGT TTTCTAACCA CAGACATGTT 
GTGGTTGATT TACAAGGTTG GGTGACTGGT AATGGAAAAG GCCTCATCTA TCTCACAGAT 
CCTCAGATTC ACTCTGTAGA TCAGAGAGAC GTCACTACAA ACTTTGGAAA GCGGGGCATA 
TTTTACTTCT TTAATAACCA ACATGCAAGC TGTAATGAAA TATGCCATCG TCTTTCTCTG 
ACGAGACCTT CACTAGAGCA AACAAGTAAG ATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_227715.5
CDS	803..4498
Translation	MNNQDAVASILHECKQVLDRLLLETPDVSTEDKGEDQRCRASLSGELRTLIQEAKEMKWPFVPEKWQYKQAMSPEDKTNLQDVIGAGLQQLLAALRASILVQDCAAAAAIVFLMDRFLYGLDVSGKLLQVAKGLHKLQPATPIAPQVVIRQARVSVNSGKLLKAEYILSSLICNSGATGTWLYRNESDKVLVQSVCIQIRGQILQKLGMWYEAAELIWASVIGYLSLPQPDKKGISTSLGILADIFVSMSKTDYEKFKKSPKVNLALLKEFDHRLLSAAEACKLAAAFSAYTPLFVLTAVNIRGTCLLSYSCSADCPPGMKSAYLREAKEAFEIGLLTKKDGELVSGKQELHSFIKAAFGLATVHCRLHGETDAVRAAKQQCSAAMGKLYTFSTSLSSQDREVLSREIMSLISQVKGHLHVQSFPNADDRSYVPESFTCGLDRPILHGHVDFQQLLETYSQHHTSVCEVFESTCRNSRGHQRDAKAEVCITTLKTETSTIDTVGVTLEKVCSQDSGSTAYSKMSEKDWEKLQRVRRRNWTHSEAFRVSLDQDTGTETELPNHSNGRADVSSKPLRDSSSSSSWSRLSGLSSSASWEEVNGAVQDTERKESSQEEHLVDTQCSTAVSEEPEGDRRICRETYLLFSKLHGVSLQTTEDHNAESSQSQLHTPALPLNSTTTCLASDAGLLEPPEGSNTTSLQSSPSCGSDAWSLASLGRFTAVTTYPSVQEEETSGIIGDFPESKYDFKDWSGEKNEGKLTEICTGPELTFASSSVDPEGDTAESTDDGPSHSLVVPGGLQGSHSMSTHRTSFSDWSVQNADSAKIGCSVKDEAVDPDASTVDEEGQMLDSTEVYSIGQDGAHRPRALRSGQSDESPKSFVNGHGPSPIFDEDFSTTEEGEELGSMLKSSQNSSSCSPWWLKSPAFSRSSSEGESSWSLLNSSRSSFGSLTGQTSQEILEARTLQPDDLEKLLAGVRHDWLLQRLENTGVLKSNQLQQAHSALLLKYSKKSELWTAQETVVYLGDYLKVKKKGKQRNAFWVHYLHQEETLGRYVGKEYKERKGLRYHLTDVERQMTAQHYVTEFNKRLYEQKIPTQIFYIPSTILLILEGRTIKGCISVEPYILGEFVKLSNNTKVVKTEYKATEYGLAYGHFSYEFSNHRHVVVDLQGWVTGNGKGLIYLTDPQIHSVDQRDVTTNFGKRGIFYFFNNQHASCNEICHRLSLTRPSLEQTSKI

Target ORF information:

RefSeq Version	XM_227715.8
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alpha-kinase 1 (Alpk1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_227715.8

ORF Insert Sequence:

ATGAATAATC AAGACGCTGT GGCTTCCATA CTGCACGAGT GCAAGCAAGT GCTGGACCGT 
CTCTTGTTGG AAACGCCAGA TGTGTCTACA GAGGACAAGG GTGAGGACCA GCGGTGCAGA 
GCCTCACTCT CCGGCGAGTT AAGGACCCTG ATCCAGGAGG CGAAGGAAAT GAAGTGGCCC 
TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCATGAGCC CAGAGGACAA AACAAACCTG 
CAGGACGTGA TTGGCGCCGG GCTGCAGCAG TTACTGGCGG CCTTGCGAGC CTCCATCCTT 
GTCCAGGACT GCGCTGCCGC CGCCGCTATT GTCTTCTTGA TGGACCGGTT CCTGTACGGA 
CTCGACGTCT CGGGAAAACT CCTGCAGGTC GCCAAAGGCT TGCACAAGCT GCAGCCCGCG 
ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAAGCCCGGG TCTCTGTCAA CTCAGGGAAA 
CTGTTGAAGG CAGAGTACAT CCTGAGCAGC CTGATATGCA ACAGCGGTGC AACTGGTACC 
TGGCTCTATA GGAATGAGAG TGACAAGGTC CTGGTACAGT CGGTGTGCAT ACAGATCAGA 
GGGCAAATTC TGCAGAAGCT AGGCATGTGG TATGAAGCGG CAGAGTTGAT ATGGGCCTCC 
GTCATAGGAT ATTTGTCACT CCCTCAGCCA GATAAGAAGG GCATTTCCAC GTCATTGGGC 
ATACTGGCGG ACATCTTTGT GTCTATGAGC AAAACTGATT ATGAGAAGTT TAAGAAGAGC 
CCAAAGGTTA ACTTGGCCTT GCTCAAGGAG TTCGACCATC GTTTATTGTC AGCTGCGGAA 
GCCTGCAAAC TGGCAGCTGC CTTCAGTGCC TACACGCCCC TGTTCGTGCT CACAGCTGTG 
AACATCCGTG GAACATGTCT ATTGTCATAC AGTTGTTCTG CTGACTGCCC TCCAGGAATG 
AAAAGTGCGT ATCTGCGTGA AGCCAAAGAA GCCTTTGAGA TTGGCCTCCT CACCAAGAAA 
GACGGCGAGC TGGTTAGTGG AAAGCAGGAG CTACACAGCT TCATCAAAGC TGCCTTTGGC 
CTCGCCACGG TGCACTGCAG GCTCCATGGG GAGACAGATG CAGTCCGTGC AGCAAAGCAG 
CAGTGCAGCG CAGCTATGGG CAAGCTCTAC ACCTTCAGCA CTTCCCTCTC GAGCCAGGAC 
AGAGAAGTCC TGTCTCGGGA AATCATGTCC CTCATCAGCC AGGTGAAGGG ACATCTACAC 
GTTCAAAGCT TCCCAAATGC AGATGATAGA TCTTATGTCC CAGAGAGTTT CACGTGTGGT 
TTAGATAGGC CCATCTTGCA TGGGCACGTG GATTTCCAAC AGCTTCTCGA AACCTACTCC 
CAGCACCATA CTTCAGTGTG CGAGGTATTT GAAAGCACGT GTAGGAACAG CAGAGGCCAC 
CAGAGAGACG CAAAGGCAGA GGTCTGTATC ACCACTCTGA AGACGGAAAC AAGCACTATA 
GATACTGTGG GGGTTACTCT AGAGAAAGTG TGCTCCCAAG ACAGCGGGAG TACAGCTTAT 
TCCAAGATGT CTGAGAAAGA TTGGGAGAAG CTCCAAAGAG TAAGGAGGAG AAACTGGACC 
CATTCCGAGG CGTTTCGAGT CTCCCTGGAT CAAGATACGG GGACGGAGAC TGAGCTACCA 
AACCACAGCA ATGGCAGGGC AGATGTCTCC AGCAAGCCTC TGAGAGACAG CTCTAGCTCG 
AGTTCTTGGA GCAGACTGTC AGGGCTCAGC TCTTCCGCAA GCTGGGAGGA AGTGAACGGT 
GCTGTTCAGG ATACAGAAAG GAAAGAGTCT AGCCAAGAGG AACATCTTGT GGACACTCAA 
TGCTCCACAG CAGTGTCTGA GGAACCCGAG GGGGACAGAC GTATATGTAG AGAGACGTAT 
TTGCTGTTTT CCAAGCTCCA CGGTGTCTCT CTTCAGACGA CCGAAGATCA CAATGCAGAG 
TCTTCACAAA GTCAGTTACA CACACCCGCC CTTCCTCTCA ACTCGACGAC CACTTGTTTG 
GCCTCAGATG CAGGGCTCTT AGAACCCCCT GAAGGATCCA ACACTACTTC TCTTCAGTCC 
AGTCCTTCAT GTGGTTCTGA TGCCTGGTCC TTGGCTAGTT TGGGCAGGTT CACAGCTGTG 
ACCACATATC CTTCAGTTCA GGAAGAAGAA ACTTCTGGAA TAATTGGTGA TTTCCCAGAA 
TCCAAGTATG ACTTCAAAGA CTGGAGTGGA GAAAAGAATG AGGGAAAACT CACTGAAATA 
TGCACGGGGC CTGAATTAAC GTTTGCCTCC TCCTCGGTAG ACCCAGAAGG AGACACGGCA 
GAAAGCACAG ATGATGGCCC ATCACACTCT CTGGTAGTCC CAGGAGGTTT ACAAGGCAGC 
CATTCAATGT CTACGCATCG GACTTCCTTT TCAGATTGGT CTGTTCAAAA TGCTGACTCA 
GCAAAGATTG GCTGCTCCGT CAAAGACGAA GCTGTGGACC CTGATGCTTC TACGGTGGAT 
GAGGAGGGCC AAATGCTTGA CAGCACAGAG GTCTACTCCA TTGGCCAAGA TGGCGCTCAC 
AGACCACGTG CTTTAAGGTC CGGTCAGAGT GATGAGAGTC CAAAGTCCTT TGTAAATGGC 
CACGGTCCCT CCCCTATCTT CGATGAGGAC TTCAGCACCA CAGAGGAAGG AGAGGAACTT 
GGGAGCATGC TGAAGAGTAG CCAGAACTCC AGCTCATGCT CGCCGTGGTG GCTAAAATCA 
CCCGCCTTTT CCCGCAGTTC GTCTGAGGGG GAAAGCTCCT GGTCTCTGCT GAACTCTAGC 
CGAAGTTCCT TTGGCTCACT GACAGGACAG ACCAGCCAAG AGATCCTTGA GGCTCGCACC 
CTGCAGCCTG ATGACCTTGA AAAGCTTCTA GCAGGGGTGA GGCATGATTG GCTGCTTCAG 
AGACTGGAGA ATACAGGAGT TTTGAAGTCC AATCAACTCC AACAAGCACA CAGTGCCCTC 
CTGCTAAAAT ATTCCAAAAA GTCTGAGTTG TGGACGGCCC AGGAAACGGT GGTATATCTG 
GGAGACTACC TGAAAGTGAA GAAGAAGGGC AAGCAGAGAA ATGCATTCTG GGTCCACTAT 
CTCCATCAAG AGGAAACCCT GGGGAGATAC GTTGGGAAAG AATATAAAGA GCGGAAGGGG 
CTCCGGTACC ACCTCACAGA TGTGGAGCGG CAGATGACAG CGCAGCACTA CGTGACAGAG 
TTTAACAAGA GACTCTACGA GCAAAAGATC CCAACGCAGA TATTCTACAT CCCGTCCACA 
ATATTGCTGA TTTTGGAAGG CAGGACTATA AAGGGGTGCA TCAGTGTGGA GCCTTACATC 
CTGGGAGAAT TCGTCAAGTT ATCAAATAAC ACCAAAGTCG TCAAGACCGA GTACAAAGCT 
ACAGAATACG GCTTGGCCTA CGGCCATTTT TCTTACGAGT TTTCTAACCA CAGACATGTT 
GTGGTTGATT TACAAGGTTG GGTGACTGGT AATGGAAAAG GCCTCATCTA TCTCACAGAT 
CCTCAGATTC ACTCTGTAGA TCAGAGAGAC GTCACTACAA ACTTTGGAAA GCGGGGCATA 
TTTTACTTCT TTAATAACCA ACATGCAAGC TGTAATGAAA TATGCCATCG TCTTTCTCTG 
ACGAGACCTT CACTAGAGCA AACAAGTAAG ATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Alpk1 ( XM_001076258.5 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_001076258.2 Rattus norvegicus alpha-kinase 1 (Alpk1), transcript variant X3, mRNA.

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CloneID	ORa15413
Clone ID Related Accession (Same CDS sequence)	XM_227715.8 , XM_001076258.5
Accession Version	XM_001076258.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alpha-protein kinase 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000070.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_001076258.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATAATC AAGACGCTGT GGCTTCCATA CTGCACGAGT GCAAGCAAGT GCTGGACCGT 
CTCTTGTTGG AAACGCCAGA TGTGTCTACA GAGGACAAGG GTGAGGACCA GCGGTGCAGA 
GCCTCACTCT CCGGCGAGTT AAGGACCCTG ATCCAGGAGG CGAAGGAAAT GAAGTGGCCC 
TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCATGAGCC CAGAGGACAA AACAAACCTG 
CAGGACGTGA TTGGCGCCGG GCTGCAGCAG TTACTGGCGG CCTTGCGAGC CTCCATCCTT 
GTCCAGGACT GCGCTGCCGC CGCCGCTATT GTCTTCTTGA TGGACCGGTT CCTGTACGGA 
CTCGACGTCT CGGGAAAACT CCTGCAGGTC GCCAAAGGCT TGCACAAGCT GCAGCCCGCG 
ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAAGCCCGGG TCTCTGTCAA CTCAGGGAAA 
CTGTTGAAGG CAGAGTACAT CCTGAGCAGC CTGATATGCA ACAGCGGTGC AACTGGTACC 
TGGCTCTATA GGAATGAGAG TGACAAGGTC CTGGTACAGT CGGTGTGCAT ACAGATCAGA 
GGGCAAATTC TGCAGAAGCT AGGCATGTGG TATGAAGCGG CAGAGTTGAT ATGGGCCTCC 
GTCATAGGAT ATTTGTCACT CCCTCAGCCA GATAAGAAGG GCATTTCCAC GTCATTGGGC 
ATACTGGCGG ACATCTTTGT GTCTATGAGC AAAACTGATT ATGAGAAGTT TAAGAAGAGC 
CCAAAGGTTA ACTTGGCCTT GCTCAAGGAG TTCGACCATC GTTTATTGTC AGCTGCGGAA 
GCCTGCAAAC TGGCAGCTGC CTTCAGTGCC TACACGCCCC TGTTCGTGCT CACAGCTGTG 
AACATCCGTG GAACATGTCT ATTGTCATAC AGTTGTTCTG CTGACTGCCC TCCAGGAATG 
AAAAGTGCGT ATCTGCGTGA AGCCAAAGAA GCCTTTGAGA TTGGCCTCCT CACCAAGAAA 
GACGGCGAGC TGGTTAGTGG AAAGCAGGAG CTACACAGCT TCATCAAAGC TGCCTTTGGC 
CTCGCCACGG TGCACTGCAG GCTCCATGGG GAGACAGATG CAGTCCGTGC AGCAAAGCAG 
CAGTGCAGCG CAGCTATGGG CAAGCTCTAC ACCTTCAGCA CTTCCCTCTC GAGCCAGGAC 
AGAGAAGTCC TGTCTCGGGA AATCATGTCC CTCATCAGCC AGGTGAAGGG ACATCTACAC 
GTTCAAAGCT TCCCAAATGC AGATGATAGA TCTTATGTCC CAGAGAGTTT CACGTGTGGT 
TTAGATAGGC CCATCTTGCA TGGGCACGTG GATTTCCAAC AGCTTCTCGA AACCTACTCC 
CAGCACCATA CTTCAGTGTG CGAGGTATTT GAAAGCACGT GTAGGAACAG CAGAGGCCAC 
CAGAGAGACG CAAAGGCAGA GGTCTGTATC ACCACTCTGA AGACGGAAAC AAGCACTATA 
GATACTGTGG GGGTTACTCT AGAGAAAGTG TGCTCCCAAG ACAGCGGGAG TACAGCTTAT 
TCCAAGATGT CTGAGAAAGA TTGGGAGAAG CTCCAAAGAG TAAGGAGGAG AAACTGGACC 
CATTCCGAGG CGTTTCGAGT CTCCCTGGAT CAAGATACGG GGACGGAGAC TGAGCTACCA 
AACCACAGCA ATGGCAGGGC AGATGTCTCC AGCAAGCCTC TGAGAGACAG CTCTAGCTCG 
AGTTCTTGGA GCAGACTGTC AGGGCTCAGC TCTTCCGCAA GCTGGGAGGA AGTGAACGGT 
GCTGTTCAGG ATACAGAAAG GAAAGAGTCT AGCCAAGAGG AACATCTTGT GGACACTCAA 
TGCTCCACAG CAGTGTCTGA GGAACCCGAG GGGGACAGAC GTATATGTAG AGAGACGTAT 
TTGCTGTTTT CCAAGCTCCA CGGTGTCTCT CTTCAGACGA CCGAAGATCA CAATGCAGAG 
TCTTCACAAA GTCAGTTACA CACACCCGCC CTTCCTCTCA ACTCGACGAC CACTTGTTTG 
GCCTCAGATG CAGGGCTCTT AGAACCCCCT GAAGGATCCA ACACTACTTC TCTTCAGTCC 
AGTCCTTCAT GTGGTTCTGA TGCCTGGTCC TTGGCTAGTT TGGGCAGGTT CACAGCTGTG 
ACCACATATC CTTCAGTTCA GGAAGAAGAA ACTTCTGGAA TAATTGGTGA TTTCCCAGAA 
TCCAAGTATG ACTTCAAAGA CTGGAGTGGA GAAAAGAATG AGGGAAAACT CACTGAAATA 
TGCACGGGGC CTGAATTAAC GTTTGCCTCC TCCTCGGTAG ACCCAGAAGG AGACACGGCA 
GAAAGCACAG ATGATGGCCC ATCACACTCT CTGGTAGTCC CAGGAGGTTT ACAAGGCAGC 
CATTCAATGT CTACGCATCG GACTTCCTTT TCAGATTGGT CTGTTCAAAA TGCTGACTCA 
GCAAAGATTG GCTGCTCCGT CAAAGACGAA GCTGTGGACC CTGATGCTTC TACGGTGGAT 
GAGGAGGGCC AAATGCTTGA CAGCACAGAG GTCTACTCCA TTGGCCAAGA TGGCGCTCAC 
AGACCACGTG CTTTAAGGTC CGGTCAGAGT GATGAGAGTC CAAAGTCCTT TGTAAATGGC 
CACGGTCCCT CCCCTATCTT CGATGAGGAC TTCAGCACCA CAGAGGAAGG AGAGGAACTT 
GGGAGCATGC TGAAGAGTAG CCAGAACTCC AGCTCATGCT CGCCGTGGTG GCTAAAATCA 
CCCGCCTTTT CCCGCAGTTC GTCTGAGGGG GAAAGCTCCT GGTCTCTGCT GAACTCTAGC 
CGAAGTTCCT TTGGCTCACT GACAGGACAG ACCAGCCAAG AGATCCTTGA GGCTCGCACC 
CTGCAGCCTG ATGACCTTGA AAAGCTTCTA GCAGGGGTGA GGCATGATTG GCTGCTTCAG 
AGACTGGAGA ATACAGGAGT TTTGAAGTCC AATCAACTCC AACAAGCACA CAGTGCCCTC 
CTGCTAAAAT ATTCCAAAAA GTCTGAGTTG TGGACGGCCC AGGAAACGGT GGTATATCTG 
GGAGACTACC TGAAAGTGAA GAAGAAGGGC AAGCAGAGAA ATGCATTCTG GGTCCACTAT 
CTCCATCAAG AGGAAACCCT GGGGAGATAC GTTGGGAAAG AATATAAAGA GCGGAAGGGG 
CTCCGGTACC ACCTCACAGA TGTGGAGCGG CAGATGACAG CGCAGCACTA CGTGACAGAG 
TTTAACAAGA GACTCTACGA GCAAAAGATC CCAACGCAGA TATTCTACAT CCCGTCCACA 
ATATTGCTGA TTTTGGAAGG CAGGACTATA AAGGGGTGCA TCAGTGTGGA GCCTTACATC 
CTGGGAGAAT TCGTCAAGTT ATCAAATAAC ACCAAAGTCG TCAAGACCGA GTACAAAGCT 
ACAGAATACG GCTTGGCCTA CGGCCATTTT TCTTACGAGT TTTCTAACCA CAGACATGTT 
GTGGTTGATT TACAAGGTTG GGTGACTGGT AATGGAAAAG GCCTCATCTA TCTCACAGAT 
CCTCAGATTC ACTCTGTAGA TCAGAGAGAC GTCACTACAA ACTTTGGAAA GCGGGGCATA 
TTTTACTTCT TTAATAACCA ACATGCAAGC TGTAATGAAA TATGCCATCG TCTTTCTCTG 
ACGAGACCTT CACTAGAGCA AACAAGTAAG ATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001076258.2
CDS	803..4498
Translation	MNNQDAVASILHECKQVLDRLLLETPDVSTEDKGEDQRCRASLSGELRTLIQEAKEMKWPFVPEKWQYKQAMSPEDKTNLQDVIGAGLQQLLAALRASILVQDCAAAAAIVFLMDRFLYGLDVSGKLLQVAKGLHKLQPATPIAPQVVIRQARVSVNSGKLLKAEYILSSLICNSGATGTWLYRNESDKVLVQSVCIQIRGQILQKLGMWYEAAELIWASVIGYLSLPQPDKKGISTSLGILADIFVSMSKTDYEKFKKSPKVNLALLKEFDHRLLSAAEACKLAAAFSAYTPLFVLTAVNIRGTCLLSYSCSADCPPGMKSAYLREAKEAFEIGLLTKKDGELVSGKQELHSFIKAAFGLATVHCRLHGETDAVRAAKQQCSAAMGKLYTFSTSLSSQDREVLSREIMSLISQVKGHLHVQSFPNADDRSYVPESFTCGLDRPILHGHVDFQQLLETYSQHHTSVCEVFESTCRNSRGHQRDAKAEVCITTLKTETSTIDTVGVTLEKVCSQDSGSTAYSKMSEKDWEKLQRVRRRNWTHSEAFRVSLDQDTGTETELPNHSNGRADVSSKPLRDSSSSSSWSRLSGLSSSASWEEVNGAVQDTERKESSQEEHLVDTQCSTAVSEEPEGDRRICRETYLLFSKLHGVSLQTTEDHNAESSQSQLHTPALPLNSTTTCLASDAGLLEPPEGSNTTSLQSSPSCGSDAWSLASLGRFTAVTTYPSVQEEETSGIIGDFPESKYDFKDWSGEKNEGKLTEICTGPELTFASSSVDPEGDTAESTDDGPSHSLVVPGGLQGSHSMSTHRTSFSDWSVQNADSAKIGCSVKDEAVDPDASTVDEEGQMLDSTEVYSIGQDGAHRPRALRSGQSDESPKSFVNGHGPSPIFDEDFSTTEEGEELGSMLKSSQNSSSCSPWWLKSPAFSRSSSEGESSWSLLNSSRSSFGSLTGQTSQEILEARTLQPDDLEKLLAGVRHDWLLQRLENTGVLKSNQLQQAHSALLLKYSKKSELWTAQETVVYLGDYLKVKKKGKQRNAFWVHYLHQEETLGRYVGKEYKERKGLRYHLTDVERQMTAQHYVTEFNKRLYEQKIPTQIFYIPSTILLILEGRTIKGCISVEPYILGEFVKLSNNTKVVKTEYKATEYGLAYGHFSYEFSNHRHVVVDLQGWVTGNGKGLIYLTDPQIHSVDQRDVTTNFGKRGIFYFFNNQHASCNEICHRLSLTRPSLEQTSKI

Target ORF information:

RefSeq Version	XM_001076258.5
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus alpha-kinase 1 (Alpk1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001076258.5

ORF Insert Sequence:

ATGAATAATC AAGACGCTGT GGCTTCCATA CTGCACGAGT GCAAGCAAGT GCTGGACCGT 
CTCTTGTTGG AAACGCCAGA TGTGTCTACA GAGGACAAGG GTGAGGACCA GCGGTGCAGA 
GCCTCACTCT CCGGCGAGTT AAGGACCCTG ATCCAGGAGG CGAAGGAAAT GAAGTGGCCC 
TTCGTGCCTG AAAAGTGGCA GTACAAACAA GCCATGAGCC CAGAGGACAA AACAAACCTG 
CAGGACGTGA TTGGCGCCGG GCTGCAGCAG TTACTGGCGG CCTTGCGAGC CTCCATCCTT 
GTCCAGGACT GCGCTGCCGC CGCCGCTATT GTCTTCTTGA TGGACCGGTT CCTGTACGGA 
CTCGACGTCT CGGGAAAACT CCTGCAGGTC GCCAAAGGCT TGCACAAGCT GCAGCCCGCG 
ACGCCCATCG CCCCGCAGGT GGTCATCCGC CAAGCCCGGG TCTCTGTCAA CTCAGGGAAA 
CTGTTGAAGG CAGAGTACAT CCTGAGCAGC CTGATATGCA ACAGCGGTGC AACTGGTACC 
TGGCTCTATA GGAATGAGAG TGACAAGGTC CTGGTACAGT CGGTGTGCAT ACAGATCAGA 
GGGCAAATTC TGCAGAAGCT AGGCATGTGG TATGAAGCGG CAGAGTTGAT ATGGGCCTCC 
GTCATAGGAT ATTTGTCACT CCCTCAGCCA GATAAGAAGG GCATTTCCAC GTCATTGGGC 
ATACTGGCGG ACATCTTTGT GTCTATGAGC AAAACTGATT ATGAGAAGTT TAAGAAGAGC 
CCAAAGGTTA ACTTGGCCTT GCTCAAGGAG TTCGACCATC GTTTATTGTC AGCTGCGGAA 
GCCTGCAAAC TGGCAGCTGC CTTCAGTGCC TACACGCCCC TGTTCGTGCT CACAGCTGTG 
AACATCCGTG GAACATGTCT ATTGTCATAC AGTTGTTCTG CTGACTGCCC TCCAGGAATG 
AAAAGTGCGT ATCTGCGTGA AGCCAAAGAA GCCTTTGAGA TTGGCCTCCT CACCAAGAAA 
GACGGCGAGC TGGTTAGTGG AAAGCAGGAG CTACACAGCT TCATCAAAGC TGCCTTTGGC 
CTCGCCACGG TGCACTGCAG GCTCCATGGG GAGACAGATG CAGTCCGTGC AGCAAAGCAG 
CAGTGCAGCG CAGCTATGGG CAAGCTCTAC ACCTTCAGCA CTTCCCTCTC GAGCCAGGAC 
AGAGAAGTCC TGTCTCGGGA AATCATGTCC CTCATCAGCC AGGTGAAGGG ACATCTACAC 
GTTCAAAGCT TCCCAAATGC AGATGATAGA TCTTATGTCC CAGAGAGTTT CACGTGTGGT 
TTAGATAGGC CCATCTTGCA TGGGCACGTG GATTTCCAAC AGCTTCTCGA AACCTACTCC 
CAGCACCATA CTTCAGTGTG CGAGGTATTT GAAAGCACGT GTAGGAACAG CAGAGGCCAC 
CAGAGAGACG CAAAGGCAGA GGTCTGTATC ACCACTCTGA AGACGGAAAC AAGCACTATA 
GATACTGTGG GGGTTACTCT AGAGAAAGTG TGCTCCCAAG ACAGCGGGAG TACAGCTTAT 
TCCAAGATGT CTGAGAAAGA TTGGGAGAAG CTCCAAAGAG TAAGGAGGAG AAACTGGACC 
CATTCCGAGG CGTTTCGAGT CTCCCTGGAT CAAGATACGG GGACGGAGAC TGAGCTACCA 
AACCACAGCA ATGGCAGGGC AGATGTCTCC AGCAAGCCTC TGAGAGACAG CTCTAGCTCG 
AGTTCTTGGA GCAGACTGTC AGGGCTCAGC TCTTCCGCAA GCTGGGAGGA AGTGAACGGT 
GCTGTTCAGG ATACAGAAAG GAAAGAGTCT AGCCAAGAGG AACATCTTGT GGACACTCAA 
TGCTCCACAG CAGTGTCTGA GGAACCCGAG GGGGACAGAC GTATATGTAG AGAGACGTAT 
TTGCTGTTTT CCAAGCTCCA CGGTGTCTCT CTTCAGACGA CCGAAGATCA CAATGCAGAG 
TCTTCACAAA GTCAGTTACA CACACCCGCC CTTCCTCTCA ACTCGACGAC CACTTGTTTG 
GCCTCAGATG CAGGGCTCTT AGAACCCCCT GAAGGATCCA ACACTACTTC TCTTCAGTCC 
AGTCCTTCAT GTGGTTCTGA TGCCTGGTCC TTGGCTAGTT TGGGCAGGTT CACAGCTGTG 
ACCACATATC CTTCAGTTCA GGAAGAAGAA ACTTCTGGAA TAATTGGTGA TTTCCCAGAA 
TCCAAGTATG ACTTCAAAGA CTGGAGTGGA GAAAAGAATG AGGGAAAACT CACTGAAATA 
TGCACGGGGC CTGAATTAAC GTTTGCCTCC TCCTCGGTAG ACCCAGAAGG AGACACGGCA 
GAAAGCACAG ATGATGGCCC ATCACACTCT CTGGTAGTCC CAGGAGGTTT ACAAGGCAGC 
CATTCAATGT CTACGCATCG GACTTCCTTT TCAGATTGGT CTGTTCAAAA TGCTGACTCA 
GCAAAGATTG GCTGCTCCGT CAAAGACGAA GCTGTGGACC CTGATGCTTC TACGGTGGAT 
GAGGAGGGCC AAATGCTTGA CAGCACAGAG GTCTACTCCA TTGGCCAAGA TGGCGCTCAC 
AGACCACGTG CTTTAAGGTC CGGTCAGAGT GATGAGAGTC CAAAGTCCTT TGTAAATGGC 
CACGGTCCCT CCCCTATCTT CGATGAGGAC TTCAGCACCA CAGAGGAAGG AGAGGAACTT 
GGGAGCATGC TGAAGAGTAG CCAGAACTCC AGCTCATGCT CGCCGTGGTG GCTAAAATCA 
CCCGCCTTTT CCCGCAGTTC GTCTGAGGGG GAAAGCTCCT GGTCTCTGCT GAACTCTAGC 
CGAAGTTCCT TTGGCTCACT GACAGGACAG ACCAGCCAAG AGATCCTTGA GGCTCGCACC 
CTGCAGCCTG ATGACCTTGA AAAGCTTCTA GCAGGGGTGA GGCATGATTG GCTGCTTCAG 
AGACTGGAGA ATACAGGAGT TTTGAAGTCC AATCAACTCC AACAAGCACA CAGTGCCCTC 
CTGCTAAAAT ATTCCAAAAA GTCTGAGTTG TGGACGGCCC AGGAAACGGT GGTATATCTG 
GGAGACTACC TGAAAGTGAA GAAGAAGGGC AAGCAGAGAA ATGCATTCTG GGTCCACTAT 
CTCCATCAAG AGGAAACCCT GGGGAGATAC GTTGGGAAAG AATATAAAGA GCGGAAGGGG 
CTCCGGTACC ACCTCACAGA TGTGGAGCGG CAGATGACAG CGCAGCACTA CGTGACAGAG 
TTTAACAAGA GACTCTACGA GCAAAAGATC CCAACGCAGA TATTCTACAT CCCGTCCACA 
ATATTGCTGA TTTTGGAAGG CAGGACTATA AAGGGGTGCA TCAGTGTGGA GCCTTACATC 
CTGGGAGAAT TCGTCAAGTT ATCAAATAAC ACCAAAGTCG TCAAGACCGA GTACAAAGCT 
ACAGAATACG GCTTGGCCTA CGGCCATTTT TCTTACGAGT TTTCTAACCA CAGACATGTT 
GTGGTTGATT TACAAGGTTG GGTGACTGGT AATGGAAAAG GCCTCATCTA TCTCACAGAT 
CCTCAGATTC ACTCTGTAGA TCAGAGAGAC GTCACTACAA ACTTTGGAAA GCGGGGCATA 
TTTTACTTCT TTAATAACCA ACATGCAAGC TGTAATGAAA TATGCCATCG TCTTTCTCTG 
ACGAGACCTT CACTAGAGCA AACAAGTAAG ATATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Alpk1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム

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